LOCUS       NZ_CP142823             1397 bp    DNA     circular CON 31-JAN-2024
DEFINITION  Enterococcus faecalis strain CF3A-343 plasmid pCF3A-343-4, complete
            sequence.
ACCESSION   NZ_CP142823
VERSION     NZ_CP142823.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38286517
            Assembly: GCF_036266595.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Complete Genome of Multi-drug Resistant Enterococcus faecalis ST620
            strain
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Chae,J.-C., Oh,J.Y., Jeong,J.H. and Kwak,S.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2023) Division of Biotechnology, Jeonbuk National
            University, Kobongro, Iksan 54596, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9b
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110x
            Sequencing Technology :: Illumina iSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/30/2024 11:12:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,890
            CDSs (total)                      :: 2,811
            Genes (coding)                    :: 2,757
            CDSs (with protein)               :: 2,757
            Genes (RNA)                       :: 79
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 63
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 54
            CDSs (without protein)            :: 54
            Pseudo Genes (ambiguous residues) :: 0 of 54
            Pseudo Genes (frameshifted)       :: 17 of 54
            Pseudo Genes (incomplete)         :: 27 of 54
            Pseudo Genes (internal stop)      :: 14 of 54
            Pseudo Genes (multiple problems)  :: 3 of 54
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142823.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="CF3A-343"
                     /host="Pig"
                     /db_xref="taxon:1351"
                     /plasmid="pCF3A-343-4"
                     /geo_loc_name="South Korea"
                     /collection_date="2020-09-14"
     gene            complement(join(1376..1397,1..743))
                     /locus_tag="VW921_RS14445"
                     /old_locus_tag="VW921_14445"
     CDS             complement(join(1376..1397,1..743))
                     /locus_tag="VW921_RS14445"
                     /old_locus_tag="VW921_14445"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="WP_329443149.1"
                     /translation="MYWQKRLDRTASTQIIEEEIQAIRKKHQHYGYRRMTQELKRRGFQ
                     VNKKKVQRLIQKLKLQVKAFTKKSRKYNSYKGTVGKIAKNLIRRRFKTSVPHQKITTDT
                     TEFKYFEADNVGIFRQKKLYLDPFMDMYNSEILSYSLSTQPNGKTVMEGLKEAISQTND
                     CPYRRTFHSDQGWAYQMNVYIQALKDNNIFQSMSRKGTCLDNSPMENFFSILKQEVYYG
                     KIYQSQNELREAIENYIYYYNHHRINEPTPKS"
                     /name="IS3 family transposase"
     gene            complement(785..1342)
                     /locus_tag="VW921_RS14450"
                     /old_locus_tag="VW921_14450"
     CDS             complement(785..1342)
                     /locus_tag="VW921_RS14450"
                     /old_locus_tag="VW921_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_002377881.1"
                     /translation="MAKYSFEFKLKLVHDYLSGQGGLRFLAKKYGFKDSSQISKWINAY
                     KELGEEGLLRSQKNKNYSVQFKLDAIELYLTTELSYQEVANLLKMNNPSLIANWLRTYQ
                     KFGIEGLSKQKGRPPTMPKKKQKESQPLPSERSQVEKLEKENRMLKIENAYLKELRRLR
                     LEDEQKMNESHESFTVSEDTFD"
                     /name="helix-turn-helix domain-containing protein"
ORIGIN
        1 ttgattcggt gatgattgta ataataaatg tagttctcaa ttgcttctct caattcattt
       61 tgactttgat agatttttcc atagtacact tcttgtttta ggatactgaa gaaattctcc
      121 ataggagaat tatctaaaca ggttcctttt cgagacatac tttgaaagat attgttgtcc
      181 tttaatgctt gtatatagac attcatttga taagcccagc cttgatcaga gtgaaaggtg
      241 cgccgatagg gacaatcatt tgtttgagaa attgcttctt tcagaccttc cataactgtt
      301 ttaccatttg gttgagtaga aaggctatag cttagaattt cactattata catatccata
      361 aatggatcta agtatagttt tttctgacga aagatgccta cgttatctgc ttcaaagtac
      421 ttaaattcag ttgtatcagt tgtaattttt tgatgaggaa cagaggtttt aaatcggcga
      481 cgaatcaaat ttttagcgat tttaccaacg gttcctttat aggaattata ttttctggat
      541 ttcttagtaa aagctttgac ttgtaacttt aacttttgta ttaatcgctg gactttcttt
      601 ttattcactt gaaatccacg tcgtttcaat tcttgggtca tccttctata gccataatgt
      661 tgatgttttt tacgaatcgc ttgaatttct tcttcaatga tttgtgttga agctgttctg
      721 tcgaggcgtt tttgccagta catgtaagta gattttggaa accttagggt ttccaaaata
      781 tcttttaatc gaaagtgtct tcggagactg tgaatgattc gtgcgattcg ttcatttttt
      841 gttcgtcctc taaacgcagt ctcctcaatt cttttaaata ggcattctca atttttaaca
      901 ttctattttc tttttctagt ttctctactt gagatcgttc acttggtaat ggttggcttt
      961 ccttttgttt cttcttaggc atggtaggtg gacgtccttt ctgtttagag agtccctcta
     1021 taccaaactt ctgatacgtt ctaagccaat tcgcaatcaa tgatggattg ttcattttta
     1081 gaagattagc tacttcttga taggacaact ctgttgttaa atataactct atcgcatcta
     1141 gcttaaattg aacagagtaa ttcttatttt tttgactgcg aagtaggcct tcttcaccaa
     1201 gttccttata ggcatttatc catttgctta tttgagaact atctttaaac ccatacttct
     1261 ttgcgagaaa ccttagacct ccttgaccag ataagtagtc atgaacaagt ttaagtttaa
     1321 attcaaaact gtattttgcc atacaaaaaa acctccaaaa gttagatttc taggtctaac
     1381 ttttgggggt cggctca
//
