LOCUS       NZ_CP142696            10610 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Klebsiella pneumoniae strain hv-CRKP plasmid p5, complete sequence.
ACCESSION   NZ_CP142696
VERSION     NZ_CP142696.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39245697
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10610)
  AUTHORS   Zhang,W.
  TITLE     Blood infection caused by a strain of hv-CRKP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10610)
  AUTHORS   Zhang,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2024) Department of Clinical Laboratory, Shanghai
            University of Medicine and Health Sciences Affiliated Zhoupu
            Hospital, No.1500, Zhouyan Rd., PuDong New District, Shanghai
            201318, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 413.0x
            Sequencing Technology             :: Illumina NovaSeq 6000; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 02/12/2024 13:09:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 14
            CDSs (total)                      :: 14
            Genes (coding)                    :: 13
            CDSs (with protein)               :: 13
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142696.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10610
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="hv-CRKP"
                     /isolation_source="Shanghai"
                     /db_xref="taxon:573"
                     /plasmid="p5"
                     /geo_loc_name="China:Shanghai"
     gene            complement(join(9639..10610,1..1209))
                     /locus_tag="VK182_RS00005"
                     /old_locus_tag="VK182_29590"
     CDS             complement(join(9639..10610,1..1209))
                     /locus_tag="VK182_RS00005"
                     /old_locus_tag="VK182_29590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="WP_331764348.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRGD
                     ANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRREHA
                     QELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALSVID
                     RACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKPNSRH
                     MLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYATLAAV
                     VLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIGQALLEA
                     KESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRYTPALLEV
                     LELRYGERDVTRREHAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALE
                     SLRQKRRILPALSVIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSIT
                     WLTWLRQAPLKPNSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKD
                     LGKFEPQRRYATLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAIN
                     DKVRLYSRIGQALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVG
                     ENFATLRRYTPALLEVLELRAAPGPRQGVWQP"
                     /name="DUF4158 domain-containing protein"
     gene            complement(1213..1773)
                     /locus_tag="VK182_RS00010"
                     /old_locus_tag="VK182_29595"
     CDS             complement(1213..1773)
                     /locus_tag="VK182_RS00010"
                     /old_locus_tag="VK182_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            complement(2305..2850)
                     /locus_tag="VK182_RS00015"
                     /old_locus_tag="VK182_29600"
     CDS             complement(2305..2850)
                     /locus_tag="VK182_RS00015"
                     /old_locus_tag="VK182_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2876..3202)
                     /locus_tag="VK182_RS00020"
                     /old_locus_tag="VK182_29605"
     CDS             complement(2876..3202)
                     /locus_tag="VK182_RS00020"
                     /old_locus_tag="VK182_29605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3560..3718)
                     /locus_tag="VK182_RS00025"
                     /old_locus_tag="VK182_29610"
     CDS             complement(3560..3718)
                     /locus_tag="VK182_RS00025"
                     /old_locus_tag="VK182_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4358..4534
                     /locus_tag="VK182_RS00030"
                     /old_locus_tag="VK182_29615"
     CDS             4358..4534
                     /locus_tag="VK182_RS00030"
                     /old_locus_tag="VK182_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4524..4868
                     /locus_tag="VK182_RS00035"
                     /old_locus_tag="VK182_29620"
     CDS             4524..4868
                     /locus_tag="VK182_RS00035"
                     /old_locus_tag="VK182_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5077..>5511
                     /locus_tag="VK182_RS00040"
                     /old_locus_tag="VK182_29625"
                     /pseudo=""
     CDS             5077..>5511
                     /locus_tag="VK182_RS00040"
                     /old_locus_tag="VK182_29625"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5546..6055
                     /locus_tag="VK182_RS00045"
                     /old_locus_tag="VK182_29630"
     CDS             5546..6055
                     /locus_tag="VK182_RS00045"
                     /old_locus_tag="VK182_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            6062..6274
                     /locus_tag="VK182_RS00050"
                     /old_locus_tag="VK182_29635"
     CDS             6062..6274
                     /locus_tag="VK182_RS00050"
                     /old_locus_tag="VK182_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6650..6778)
                     /locus_tag="VK182_RS00055"
     CDS             complement(6650..6778)
                     /locus_tag="VK182_RS00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            6941..7165
                     /locus_tag="VK182_RS00060"
                     /old_locus_tag="VK182_29640"
     CDS             6941..7165
                     /locus_tag="VK182_RS00060"
                     /old_locus_tag="VK182_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            7149..7415
                     /locus_tag="VK182_RS00065"
                     /old_locus_tag="VK182_29645"
     CDS             7149..7415
                     /locus_tag="VK182_RS00065"
                     /old_locus_tag="VK182_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7485..7706)
                     /locus_tag="VK182_RS00070"
     CDS             complement(7485..7706)
                     /locus_tag="VK182_RS00070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_331764346.1"
                     /translation="MVVGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPD
                     VNNMTLLVKNNGYIIVKTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          4193..4272
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3610..4007)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4859..6390
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gcgcagttcc agcacctcca gcaaggccgg cgtgtaacgg cgcagggtgg cgaagttctc
       61 gccgaccagg tgcaggtggt cgaagccttc cggccgggcc agcagctcgg cctcgctgac
      121 gctctcggtg aactcgtccc agggaatcac cgcctcgatg gcggcatagg ggtcgctgcc
      181 gctttccttc gcttccagca gcgcctggcc gatcctggag tacaggcgca ccttgtcgtt
      241 gatcgccttg ccctgcttct ggaactgctg ctgatgcttg tgcttcgcgc cgctgaacag
      301 cttgaccagg atgcggtcat gcagatcgac cagctcatcg atcacggtcg cggtgctctc
      361 cagcaccacg gcggccaggg tcgcgtagcg gcgctgcggc tcgaacttac cgaggtcttt
      421 gggcgtcatc tgcccaccct cgcgggccag cttgagcagg cggttctggt ggatgtgccg
      481 gcccaggcct tcgggcaagt ccaccaactg aaatgtcttc agccgctcga tgtgttccag
      541 catgtgccga gagttgggtt tcagcggtgc ctggcgcagc caggtcaacc aggtgatgct
      601 gctgccggcc ttgagcttca acagctcgtc cagcttggcc cgatgcgagt ccgtgagtgg
      661 ttcgaccagg gcgcggtaga cccgccgatt ggctcgcgca atggcttccg agcaggcccg
      721 gtcaatcacg ctcagcgccg gcaggatgcg tcgtttctgc cgtaggctct ccagggcctg
      781 accggccagc agcaagcctt tgtcggtctg ctgggccagc tcggttagct cgcgcaccag
      841 ggcgcggaag tcggacaggc cgaacggggc cagttgcagg taggtgcgca gttcctgggc
      901 atgctcgcga cgggtcacgt cgcgctcgcc gtactttgcc cagctcgccg gctcggcctg
      961 gacttgcttc gccacccaca ggatgaccgg ctcgggcagc tcgctgtcgg ttcccagcgc
     1021 gtaaccgggg tagcgcagca ggcagagctg cacggcgaag ccgaggcggt tggcgtcgcc
     1081 gcgtcgctgg cggatcagcg acaggtcgga gtcgttgaag gtgtagtagc ggatcaggtc
     1141 atcctggctt tccggcagcg caagcagggt gtcccgctcc gtggccgaga ggatcaagcg
     1201 acgcggcatg tgtcagtcgt ccgtgcggag gtactggtag agggtttccc ggctgatgtt
     1261 gaactcgcgg gcaagctgcg ccttgggctc gccggccgtc gctcgctgcc gcagggtagc
     1321 agcctgctca tcggacaggg ctttcttgcg gccccggtac gcgccacgct gcttggccaa
     1381 ggcgatgccc tcacgctgcc gctcgcggat cagggcgcgc tcgaactcag cgaaggcccc
     1441 catcaccgac agcatcaggt tggccatcgg cgagtcctcg ccagtgaaca ccaggccctc
     1501 cttcaggaac tcgatgcgca cgccgcgctg agtcagcttc tgtaccaagc gacgcaggtc
     1561 atcgaggttg cgggccagcc gatccatgct gtgcaccacc actgtatcgc cttcgcggac
     1621 gaagctcagc agcgcttcga gctgggggcg ctgggtgtcc ttgcccgatg ccttgtcggt
     1681 gaacaccttg ctcacctggg tttgttccag ctggcgttcc gggttctggt caaagctgct
     1741 gacccggacg tagccgatgc ggtgcccctg cacgatgtct ccttggttga aggcggctta
     1801 agtgcacttt ctgttccgtt gtgcctcaaa gcccatttct gtcaggctga aatctataac
     1861 cttcgcgggc atgtgtcaaa aaatgggaaa gcagactcta ttctgacgaa gcggcgcggc
     1921 cctgcctgac atcaagttag ggtatagcct agattgacat gcgcgatgca acccttaact
     1981 tgcttgcacc tatcgtttcc atgctagctt tatcgtaacg ctaaggaagc tctgaaaaag
     2041 cccatgtttc gcgaaatcac cgctctgtaa cccgcatggt tgctgggatg catttctcga
     2101 gcgggggctt tttcagacct tccttagctt agcgtacgat tttttccgaa ttctgcggtt
     2161 ccccctagta agcggtgtaa ccgtcagcaa aagatcgacc ggggaatata aatgctagga
     2221 acgcaattaa ccaatacata gttgagaatc ttggtattac acatttcact atcctctctt
     2281 tcatttttcg ttcaccatac tttgttatct gatttttaat ttttctattt tataaaccaa
     2341 gtctctacca aaaaggcgaa aattatcctc aaataataat aattgtttaa gaacatccat
     2401 gaaagcagct gcttcaccct cagttatggg agctactcta tataacatat aacctgagtt
     2461 aagggtttct tttatacttt cttcaatgct cattataaat attagtggat tatcattttt
     2521 cttcccccat gggtttctaa ttaatccttc gatgtactca ggtcctttgc gtcctattgc
     2581 ctgcgcaata ttttttattt catctgcgtg agggctttgt tgtacagctt tgtttatgcc
     2641 tgcttgagtg attttcagag actctacacc gtgtatgttg tatgagataa aatgtaataa
     2701 attaagatgc aatgcaatta atccattttt gtttacatct gagtatttgg ataaaaaacg
     2761 taatgaagtg ttaactctcc cttgaagcct aacaattggg agtgctgcct tgtatatttc
     2821 cttctccgtt aaataccgtt taaaaaacat aattctccgc ctccgtatag cgtgcctatt
     2881 ttatttcgtc gtaaaactta gctctgaaat attcctttag aaccatcatg tcattaataa
     2941 ttgaatctat ctcatccatt ttttcttggt attcttttaa tggtgaaggg agcggtaact
     3001 caatatttga aaagtgattg gcgagatagg gatcaaggtt tctcatccat tcaggctttt
     3061 cgccaatgcg ttcttcaact gaaagtgaac ctgataatct ctggttaata agatcactta
     3121 gactcatttt ggttttattc gaaagccgtt gtaaggattg ctttaatcct atatctagca
     3181 tgatatttgt tctttctctc attacccccc cttgctattg cgttttttgt tgcgcacaaa
     3241 gctaccattg cgcattaaga aacgcaataa aaaaggaggg agggagtggt ttgtacatga
     3301 tgtgcagtgc gctgagggca tcagtggcgg gtgtttttcc gtgggatgga tggagctccg
     3361 gggataacag aactgacgat catttaaagg atcttcttga gatccttttt ttctgcgcgt
     3421 aatctcttgc actgtaaacg aaaaaaccgc ctggggaggc ggtttgatcg aaggttaagt
     3481 cagttgggga actgcttaac cgggtaactg ggtatacgag accgcagata ccaaaactgt
     3541 tctttcagtg tagccgcagt taggcaccca cttcaagacc tctcaatatc tctctcgtat
     3601 atatccagtt accaatggct gctgccagtg gcgtttttgc gtgtccgtcc gggttggact
     3661 caagatgata gttaccggaa agggcgcagc agtcgggctg aacggggggt tcgtgcatac
     3721 agcccagctt ggagcgaacg acctaaaccg agccgagata ccagcagcgt gagctatgag
     3781 aaagcgccac gcttcccgaa agggaaaaag gcggaacagg tatccggtaa acggcagggt
     3841 cggaacagga gagcgcacga gggagccgcc ggagagaaat caccggcatc tttaagtcct
     3901 gtcgggtttc gtcctcctct gacttgagcg tccatttctg tgatgctcgt caggggaggc
     3961 ggagcctatg gaaaaacggc aacgccgcat ccttttctcc ggccctttct gatttgagcg
     4021 cctttgcttt atcccgttat ccgtgagtcg gtcacgcccg ccgcagccga acggcaggag
     4081 cgcagctacc gagtgagcga ggaggcgtca tttcatctgt ccgtgcatgg tattgcggac
     4141 gctcctgagc cacatatcac acctgccaca tgaggcactt tcatgaatcg tgctctcatg
     4201 ccaacatagt aagccagtat acacagcgta aacgctgtga ctggttcagg gctgcgcccc
     4261 gaaacccgct aacaccactg acgcgctggc gcttgtccaa cgccggagcg acccggaaaa
     4321 cttttccgtg ccgtccggcg tttgcgaagg agggataatg cagaagccga agaagaagcc
     4381 gctcgtcgta aaaaaaagat cctggcatcc ggcagtgatg gcaaatctga tgcgtcaggc
     4441 acaggaggaa gattaccgcc gggagcagat ggcagagctt gagcgttcag cccggtcaca
     4501 ttttgcggat atcggaggag aggatggcgg ttaagcgtac ccgtttttta ggtatccggg
     4561 tgaccgatgg ggagtaccag cagctgctgg agcgctgcaa tggaaggcaa ctggcggtgt
     4621 ggatgcggga aacctgtctc gatacgcgcc cggcgcggtc gttgcggctg ccgtccatcg
     4681 atccggtttt gctgcgccag cttgccggga tggggaacaa cctcaaccag atcgcccgga
     4741 aaattaacgg cggccagtgg tcaggcgctg accgggttca ggtggtggcc gcgctgatgg
     4801 ccattgatgc cgaactcgag cggctgcggc atgccgtgct ggaaaagggg gcagacgatg
     4861 atcgttaagt ttcatccgcg agggcgaggc ggcggtgccg ggccggtgga ttatctgctg
     4921 gggaaagacc gccagcgcga cggggccatc gtcctgcagg gaaagccgga ggaggtccgg
     4981 gagcttatcg acgcctcgcc ctacgccaaa aagtacacct ccggcgtcct gtcttttgct
     5041 gaacaggatt taccgcccgg ccagcgtgaa aagctgatgg cgagcttcga gcgggttctg
     5101 atgcccggac tcgataaaga ccagtacagc gtgctgtggg ttgaacaccg ggacaagggg
     5161 cggctggaac tgaattttct ggtaccgaac acggaactgc tgaccggcag gcgtctccag
     5221 ccgtactacg accgggcaga ccgtccgcgc atcgatgcgt ggcagaccgt ggtgaacggg
     5281 cgactggggc tgcacgaccc gaacgcaccg gagaaccggc gcgcgctggt gacgccatct
     5341 tcgttaccca aaacgaagca ggaagccgca gaggctatta cgcggggctt actggccctc
     5401 gcctcgtcag gggagcttaa aacgcgtcag gacgtcactg aggcgctgga aagcgcaggt
     5461 tttgaggtcg tgcgcaccac aaagagcagc atcagcattg ccgacccgga cggagggcga
     5521 aacatccgac ttaagggagc catctatgaa cagtctttta acgctggcga aggacttaga
     5581 gcagaaatcg aaagcgcagc agcagagtac cggcgaaatg ctgaaagccg cattcagcga
     5641 gcacgagaag tctgtcagag cggaactgag cgaaagcggg aagagaatca gcgccgccat
     5701 ccgcgcccac gagcagggta tgagcgcggc catgcagtcg aaccgcctga gcgtgatgcg
     5761 tatggtcggg cggacatggc tgaccatcac gatggtgtca ggactgctgt tcgccagcct
     5821 gagcggggtg ttgtggtatc aggggagctt gatagcgtca aatctggccg aaatcgaccg
     5881 gcagaacgca gcgctgtcga agctgaacgc gaagacctgg ggcgtgacgt acctggaaga
     5941 cagcaacgga cgttttctgg tgctgccgaa ggggacggcc gtagacagga cgcagagctg
     6001 gacggtcggg aacgggcggt cgaagcagaa cgcgctgaga ctggtgaagg agtaagtgcc
     6061 gatgaccgag ctggaaaaac agttgctgag cgcattagag cagctacagc aggactactc
     6121 gaaaaggctg gacgagtggg agagcgcctt cggggaatgg cggaaaatgt cagggcttat
     6181 gcaacgggag aacgcggcgc tgagcgagcg cgtgacgcgc ttgagtcagc aggtggaacg
     6241 cttgagcggg caactgcagc gtttgagccg gtagtgcagc gtcatgagat ggccgtggcc
     6301 gcagaacggg cgcacgagca acggcagcac gaaaaggaac tgacggaagc gcgttcccgg
     6361 cagcgaagct atgacggtcc gtcgctgggc tgaaatgcag gaaaacaggg ttctcatcgg
     6421 gcatttacgc tgaaaccgga tgtcattcac ctggctgcat ggctatgcag ccaggtaaaa
     6481 tttatctgtc gcgaacgggg cggtttatcg ggtggtttgt tgccggtttt acctgactgc
     6541 caccccgttc gcggcgaacc cgtccggggc ggtgcgggca acgggtgtta tgtttaatca
     6601 gcgtgaacgg cagatacaaa aaagccccgg agggctttta actactgact tatttttttg
     6661 aagatttgct gggtttcaat ttgtcagtat ttcccggcgt ttttttattg tcacgctggc
     6721 gtttatcagg ttcccctgtt gaatcggcaa ttcgtttcgg acccggctta agacccataa
     6781 tcgttatcct ttcaatagtt aagaggaaag ccctcaagct agatatattg accgcgttac
     6841 ggattatcaa gaggggctaa caagattatt ttttgttctt ttaggttgat tgtggtactg
     6901 ttgatataca tgtatagcaa atggtgttcg ggaggtttat atgcttgcta tccggttatc
     6961 tgatgagatt gagtcccgtc tggactcgct ggcgaagcaa accggcagaa caaagacgtt
     7021 ttatgcgcgg gaagcaatac tggcgcatct ggaagacctg gaggattatt atctttcagc
     7081 agaaactgct gcacgcgttc gccgtggtga tgaagcagtg cattcgtctg aagacgtgag
     7141 gaagtcactt ggtctggacg attaactatt ccgatcgggc gctcaaatcg ttacgcaaga
     7201 tggacaaaca gaacgcacga cggattgtgg attttatgag tttacgcatt gcagttgctg
     7261 ccgatcctcg ccagtcaggg aagccgctca aaggtgagct gggcgagttc tggcgctatc
     7321 gggtgggaga ttatcgcgtt ctgtgtgaga tccgagatga cgagcttgtt atccttgccg
     7381 ccacgattgg acatcgccgc gaagtttacg actgaagccc gctatgcggg cttttttgat
     7441 gccgacaaca ggtattatgg taaatactcg actaactacg tgttctactg tttgtcggtt
     7501 ttagttaacc agcaggttgt cttaacgata atgtagccgt tatttttaac taataaggtc
     7561 atattgttaa cgtcaggcat aataaaagac attatggcct cagagttatc atcagaagca
     7621 gctctctgca ttactacttc gattcccttt tctttgaatc tataagtatc cagttgccta
     7681 ttaaacttag aaagacctac aaccatactt agtttgttat ctttaacagt aagagatgct
     7741 tcctgagtac cattacatcg gtagtagggg agcccgcaga attcggaaaa aatcgtacgc
     7801 taaggttttc cgggcatccg taagggccga aacttcccgt cttccagtct gcggctctgc
     7861 cgccagacgt aatccgcacg gttaggtttg atgtgcctcc ccagcccagc ggcgacagga
     7921 attgcaggca gctcgccgtc cacccgcttg ccgtccacga ccaccccctg ggtggcgcag
     7981 cgttccaggg tacaccgtgt tcccgcagca cgatagccgc cgttcaccag gttgaggccg
     8041 ctggcccggt agcgcctggc tgctcacgac gagcctccga tccctgattt ccccaaggcg
     8101 gggttgaaga acaccgccct ggccagcgag ttgcgcgcct cacctttgtt tcagagccga
     8161 gcatgcacgc ggcggcgcag ttcaacactt tgcagccagt ccaggaaaga acatcggcgc
     8221 tacgaatccg gcccagctcg cgcagggcca cggccagtcc gttctggcgc gggtagctgc
     8281 cagagcttgc tcagcatcag cgaggcggtg acggtgcagc tgcttgatcg agctggccag
     8341 gcgcaaggat gtcgtcccag tgtgcacgca ccgtggcttg atgttcaggg tggccgccga
     8401 tcaagtcagg cgcaacgtcg ggtacggctt gcacgccctg cggcacgtac agcttggttt
     8461 cgagccgagg tcgcggatgc ccgcggcggc gcgaacgtga acctagccca aagttgcatc
     8521 agggcaaaga cgtgattcgt gaaggccgga gccgtggtcg gtgtagtgct cctcgaatcc
     8581 gcaggtcccg gacacgtggt acagcaggcc gatcgagcac gataggtgga atcagcggga
     8641 cgccgacatt tcaccacgcg ggtgctgaac ggcgcgtacc tggtcgagat atcgggtata
     8701 gaacaagccg acccggctcg cctaacctgt acttcgggtt gacgtgcccg gtggctcatc
     8761 agccccgggg tgggccaccc gcgcggaagc gctggccatc ggagatggat gtcttgcgtg
     8821 cgccccagtg ggcggcaaag gcgtggcgat acggtggttg accagtctcg tgccataggc
     8881 cgccgaatag gtttcgtcgt cgcggatgat gccaaaggct tgcagccagg acagcttggc
     8941 gtaggtcagg ccgggctcga ctcggccatc cttgtatcag cctcaggttg aacgcatcac
     9001 cgatgaattg cggacagcac taagcagcaa cgtctggtct ttggcctcgc cccgtccctt
     9061 caagtgggtg aagtggcgga ctgaagcccc gtcccatcgt cacgtccgat cagcagtttc
     9121 ggtgatcttg atgcgcggcg agtaactggc tggtttgtcc cgatcagcgc ctgcgcaccg
     9181 aaaccggcac cgccgcatcc agcgggtgat tttcatgccc tgactcggtg aggatggcat
     9241 cggccagctc gttgtcccct tggccaggcg ggtgagacgg tggccaactc tcgtccagca
     9301 gctgcaaacg cacttgcact ttccaggtac tgggtccgca gtttcgggtt gatcgccagg
     9361 ggcagggcct gctcgcgctt gagtgcggcg aactctcgcc ggcagcaggt cgtcgtcgaa
     9421 gtctcgcgac tgccgcgagc cacttttgac ccagatgtcg ccggagcgca ggcgatcttc
     9481 tgcagctcgg acaagggcgc agattgatcg taagaatttc cggtcgaggc cttccggggt
     9541 gattcaccag gcgctttcca gcgcggctat gatgtaaggc cgtgggtgca tcggccggca
     9601 cctgcagcag ggttgtcgcg ttcatctcac gcagggtctc acggctgcca cacgccttgg
     9661 cgcggccccg gcgcggcgcg cagttccagc acctccagca aggccggcgt gtaacggcgc
     9721 agggtggcga agttctcgcc gaccaggtgc aggtggtcga agccttccgg ccgggccagc
     9781 agctcggcct cgctgacgct ctcggtgaac tcgtcccagg gaatcaccgc ctcgatggcg
     9841 gcataggggt cgctgccgct ttccttcgct tccagcagcg cctggccgat cctggagtac
     9901 aggcgcacct tgtcgttgat cgccttgccc tgcttctgga actgctgctg atgcttgtgc
     9961 ttcgcgccgc tgaacagctt gaccaggatg cggtcatgca gatcgaccag ctcatcgatc
    10021 acggtcgcgg tgctctccag caccacggcg gccagggtcg cgtagcggcg ctgcggctcg
    10081 aacttaccga ggtctttggg cgtcatctgc ccaccctcgc gggccagctt gagcaggcgg
    10141 ttctggtgga tgtgccggcc caggccttcg ggcaagtcca ccaactgaaa tgtcttcagc
    10201 cgctcgatgt gttccagcat gtgccgagag ttgggtttca gcggtgcctg gcgcagccag
    10261 gtcaaccagg tgatgctgct gccggccttg agcttcaaca gctcgtccag cttggcccga
    10321 tgcgagtccg tgagtggttc gaccagggcg cggtagaccc gccgattggc tcgcgcaatg
    10381 gcttccgagc aggcccggtc aatcacgctc agcgccggca ggatgcgtcg tttctgccgt
    10441 aggctctcca gggcctgacc ggccagcagc aagcctttgt cggtctgctg ggccagctcg
    10501 gttagctcgc gcaccagggc gcggaagtcg gacaggccga acggggccag ttgcaggtag
    10561 gtgcgcagtt cctgggcatg ctcgcgacgg gtcacgtcgc gctcgccgta
//
