LOCUS       NZ_CP142687             5594 bp    DNA     circular CON 12-FEB-2024
DEFINITION  Bacteroides fragilis strain ATCC 43858 plasmid pBFP53, complete
            sequence.
ACCESSION   NZ_CP142687
VERSION     NZ_CP142687.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39465054
KEYWORDS    RefSeq.
SOURCE      Bacteroides fragilis
  ORGANISM  Bacteroides fragilis
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales; Bacteroidaceae;
            Bacteroides.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Myers,L.L., Shoop,D.S., Stackhouse,L.L., Newman,F.S., Flaherty,R.J.,
            Letson,G.W. and Sack,R.B.
  TITLE     Isolation of enterotoxigenic Bacteroides fragilis from humans with
            diarrhea
  JOURNAL   J Clin Microbiol 25 (12), 2330-2333 (1987)
   PUBMED   3429625
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Lee,E. and Yeom,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2024) Microbiology and Immunology, Seoul National
            University, College of Medicine, 103, Daegak-ro, Jongro-Gu, Seoul
            03080, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                :: JAN-2024
            Assembly Method              :: FLYE v. 2.9
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 626.0x
            Sequencing Technology        :: Oxford Nanopore; NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 02/12/2024 13:09:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 7
            CDSs (total)                 :: 7
            Genes (coding)               :: 7
            CDSs (with protein)          :: 7
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142687.1.
            Bacteria and source DNA available from SNU_yeomlab_ATCC43858.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Bacteroides fragilis"
                     /mol_type="genomic DNA"
                     /strain="ATCC 43858"
                     /isolation_source="infant with diarrhea"
                     /host="Homo sapiens"
                     /db_xref="taxon:817"
                     /plasmid="pBFP53"
                     /geo_loc_name="USA: Arizona"
                     /collected_by="LL Myers"
     gene            complement(join(4599..5594,1..54))
                     /locus_tag="VVD37_RS00005"
                     /old_locus_tag="VVD37_21310"
     CDS             complement(join(4599..5594,1..54))
                     /locus_tag="VVD37_RS00005"
                     /old_locus_tag="VVD37_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_005799258.1"
                     /translation="MCKDRTNITNCKESPLKNGSDSLKKKAKVKIISHNFTRKLSEKNP
                     DSKLMKAYMDAHVCSNVYTVQGGKAHSHYCHRAFCPICQRIQTAQNVKKFLPVMKYLAS
                     EGREFYFVTLTLQNCVTDDARVLRDFMRRCNRMWADGIRTKHKFRSLGISGVIKKECTY
                     HVVKKDLFSFHYHFHIIVDSLEAAKYVVAQWKKLHGSTVADARFQKYKKIEDFENAAIE
                     VFKYASKASVSKSKGRDGKKKIQINYKALDMIYTAMHGMQRMSSFGQFRTMIGDEALND
                     FRDEDLVLNIEGLNVEDGCYTWYMSVKDKVADWYNMETGEALCCYKVTRKDELLQDIYL
                     DEDIFPNSG"
                     /name="protein rep"
     gene            complement(549..947)
                     /locus_tag="VVD37_RS00010"
                     /old_locus_tag="VVD37_21280"
     CDS             complement(549..947)
                     /locus_tag="VVD37_RS00010"
                     /old_locus_tag="VVD37_21280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799260.1"
                     /translation="MKPTDYIEWDNLKDIPFFLCQVVEDREKQDLDIYYLGKRVLHDYD
                     HVGHYLRTAVILFRRVKSRTADWVNLRNLWTLRNCVRENYNHGIGMNDLIFGENFDGDN
                     LDTLTPLTKKRFDFLCKRIKELDPYATI"
                     /name="hypothetical protein"
     gene            complement(984..1304)
                     /locus_tag="VVD37_RS00015"
                     /old_locus_tag="VVD37_21285"
     CDS             complement(984..1304)
                     /locus_tag="VVD37_RS00015"
                     /old_locus_tag="VVD37_21285"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799262.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003723 - RNA binding [Evidence IEA]; GO:0004521 - RNA
                     endonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="WP_005799262.1"
                     /translation="MVEQYEVYWVELDPTRGGEMAKTRPCVVVTPSDLNMYLTTVVIVP
                     ITSTIRNYPYRVLCSVAGREGEIATDQIRTVDKSRLKRKIGDLNFSEIKRLREVFQQMF
                     CE"
                     /name="type II toxin-antitoxin system PemK/MazF family
                     toxin"
     gene            complement(1298..1546)
                     /locus_tag="VVD37_RS00020"
                     /old_locus_tag="VVD37_21290"
     CDS             complement(1298..1546)
                     /locus_tag="VVD37_RS00020"
                     /old_locus_tag="VVD37_21290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771457.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="WP_005812783.1"
                     /translation="MVTNIIQIGNSKGIILPSEVLKQLRLSLKSAVSISLDGNNIVIKA
                     QPRQGWAEAAKRAHENGDDELLIPDVFEDEKFEDWTW"
                     /name="AbrB/MazE/SpoVT family DNA-binding domain-containing
                     protein"
     gene            1952..3202
                     /locus_tag="VVD37_RS00025"
                     /old_locus_tag="VVD37_21295"
     CDS             1952..3202
                     /locus_tag="VVD37_RS00025"
                     /old_locus_tag="VVD37_21295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBU/pMV158-type mobilization protein"
                     /protein_id="WP_005799237.1"
                     /translation="MAKTCIRVEACNIGSSERHNLRSKELDYIRPELTHRNEQWVECSI
                     AEVHRDITEKYKEATGQGLQKKATPIREGVIVISEETTIQQLQDLAEKLEERFGVHAFQ
                     IYTHKDEGANVWDGKEEAWKPNYHAHMIFDWTDGHTGKTVKLNRHDMAEMQTITAECLN
                     MERGVSSDKKHLSAMQYKNKMETEKAEQLQKDIEQLNRAYTAGTEKITTVQKELSTAQK
                     ELNSMKTDIHINEAKEAAAKAGKTVFNAVTSVFNVGEVKRQAKEIETLKKENYNLSFKN
                     QNLEGQLRTNNIEMRRVQETTDRQIKAEASKLKPITNLFPEMENAQENIEELQTMGIQN
                     KDIRQLLIGKEIYYTGNLYDKDKRKSYQVKNVKIDISKSQNGITTIWLNNIHFKNFLKE
                     LWNTLQKTLDNGNWLHR"
                     /name="NBU/pMV158-type mobilization protein"
     gene            complement(3272..4042)
                     /locus_tag="VVD37_RS00030"
                     /old_locus_tag="VVD37_21300"
     CDS             complement(3272..4042)
                     /locus_tag="VVD37_RS00030"
                     /old_locus_tag="VVD37_21300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016274503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005799241.1"
                     /translation="MKKVMFMFISLLTVLGVCSCNDNEVIEPVVSDSVSDMEVLSRFVD
                     VNEITNEYYFNENKKTRALSYVTGSDWQDLEKVSPLSIEKYKNNLQVLNAQVASAISNP
                     NTAYVVFSVNGKTLVKKVKEDANFDFSVFRDVVTETRAVLPSLSINGGSQSTTGVFYDS
                     SRTLKMQVDLNASIQNNYYFFEVLNPNAKPSPDDNITTPESVAFSGTGPLWSNTFTWTS
                     YWDANVPGQGFKWEFKGKGTTPSFGFIANCTFSR"
                     /name="hypothetical protein"
     gene            4134..4595
                     /locus_tag="VVD37_RS00035"
                     /old_locus_tag="VVD37_21305"
     CDS             4134..4595
                     /locus_tag="VVD37_RS00035"
                     /old_locus_tag="VVD37_21305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005812785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005812785.1"
                     /translation="MNRIIHISLLISTVSLFLISNTGCVEKQGYYNHGEESIISLICDI
                     TWAGKKTTDENGSVWQGTYKFNKNGTYTRTNIEIDKQGNKKEANIYGQWSFGDPSFSTI
                     YFGGEHYWDIDELTKNKFSFYDRSGKFGDPFMNREYIELTPYQENNTTN"
                     /name="hypothetical protein"
     Typing          complement(4600..5594)
                     /name="rep_cluster_870"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.799"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1953..3199
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gttttttaac ggactttctt tgcagttggt tatatttgtt ctatctttgc acatatgaaa
       61 ttttgatgtg gctttcgttt ggtatccgcc ccgccaagaa cgaacacaaa cgattgctaa
      121 ttcttaaaat acaaagcccg ttggttactg ccttcgggct ttcgtttttt ctcagcccaa
      181 aagtaggtaa ttcattccaa accgccaaac atcattcaaa aaacctacat ataacaagtc
      241 aaaaagcatt taccacgaaa ctttctagtt tatctagtat gggggctcgc ccccattccc
      301 ccggctgcgc aattctgaca caaatacact actaaatcca caacagacaa agatacttcc
      361 ggaaaaaaaa ctatctttgc cgtacacaaa gacaaccctt gccgagcgat acgggagagt
      421 tgcaaaaaaa acaccggagt gaattgacgg atatccgaca tctccggtgg tatttttttt
      481 cttatccccc tcccgtcccc caaaggggga gcacacaaag acaagccctg ccttcactca
      541 atggagtgtt aaatagttgc atatgggtca agttccttaa tccgcttaca caaaaaatca
      601 aagcgtttct ttgtaagtgg tgtaagcgta tcgagattat ctccatcgaa attctcccca
      661 aaaataaggt cgttcattcc tatgccgtga ttgtagttct cacgcacaca gttgcgcagc
      721 gtccaaaggt tacgcaggtt tacccagtct gctgtgcggc tctttaccct gcggaacaag
      781 ataacggcag tccgcaaata gtgccccaca tggtcgtaat cgtgcaagac acgtttgccc
      841 aaatagtaaa tatccaaatc ttgtttttct ctgtcctcga ctacttgaca gaggaagaat
      901 ggaatatcct tcaggttatc ccattcgatg tagtcagttg gtttcataat gtcttatttg
      961 catttttctt tccgtacttt ccattattca cagaacattt gctgaaatac ttcacgtaac
     1021 ctttttatct cactgaaatt caaatctcca atctttcttt tcagccggct tttatccacc
     1081 gtgcgaattt gatcagtggc tatctctcct tcacgcccgg ctacagaaca cagaacccgg
     1141 taaggataat ttcttatcgt tgaggttatc ggaacaatga ctactgttgt aaggtacata
     1201 ttcaaatctg aaggagtgac aactacacat ggccgtgtct ttgccatttc acccccacga
     1261 gtagggtcta actctaccca atacacttca tactgttcta ccatgtccaa tcctcaaact
     1321 tttcatcctc aaacacatca gggataagca actcatcatc cccattctca tgggcacgtt
     1381 tggcagcttc cgcccatccc tgccgtggtt gtgcctttat cacaatattg ttcccgtcca
     1441 aagaaatgct cacagccgat ttcaaggaca aacgcaactg tttcaatact tcggaaggaa
     1501 gtattatccc cttgctgttt ccaatctgaa taatattcgt taccataatg ttataacttt
     1561 ataattgatt atcacgatac aaatatatgt aaagttataa caataacaaa aggaaaaaca
     1621 acttttttcc attttcaccg agaaccctgt caatggctgc actcgtcggg agtgttggtg
     1681 cacccccttg ccgtgcagcg ctccccgacc gatgattttt tattgtgcct tgtgtccttt
     1741 tcccttttgt ccggcttctg ttcccaagca catccggaac acatggcaga gcgcttgcaa
     1801 atcccgtccg gaaggcttac ctttgcaaaa ggttatagca cactataacc cctaaagggg
     1861 ttgtgtgcga ggggtgtccc ctcgaccccg tgcgatgtga cgtttcacgc cacatcttct
     1921 aaacagacat acacacctaa aacctaaaga tatggcaaag acatgtatca gagtggaagc
     1981 ctgcaatata ggcagctcgg aacgtcacaa cttaagaagc aaggagctcg actacatccg
     2041 tccggaactc acacaccgca acgaacagtg ggtagaatgt tccattgccg aagtacatcg
     2101 ggacataacg gaaaaataca aggaagccac cggacaagga ttacagaaga aagctacccc
     2161 aatccgggaa ggggtaatag taatcagcga ggaaaccaca atccagcagc ttcaagactt
     2221 ggccgaaaaa ctggaagaac gctttggagt gcacgccttc caaatctaca cccataagga
     2281 tgaaggcgca aacgtatggg acggaaagga agaagcatgg aaaccgaact atcatgcgca
     2341 catgatcttt gactggacgg acggccatac aggaaaaact gtaaagctga acagacacga
     2401 catggcggag atgcagacta taacagcaga gtgtctcaac atggaacgtg gagtttcttc
     2461 agacaagaaa cacttgtcgg ccatgcaata caaaaacaaa atggaaacag agaaagctga
     2521 acaacttcaa aaagacatcg agcaactaaa cagggcatac actgccggaa ccgagaaaat
     2581 caccacagtc caaaaggaac ttagtacggc acaaaaagag ctaaacagca tgaaaaccga
     2641 catacacatc aacgaagcca aagaagctgc cgcaaaggcc ggaaaaaccg tatttaatgc
     2701 cgttacaagc gtctttaatg taggggaagt aaaacgacaa gccaaagaaa ttgaaaccct
     2761 caaaaaagaa aattataacc tctcattcaa gaatcaaaat ttagaaggac aattaagaac
     2821 caacaacatc gagatgcgaa gggtacagga aacaaccgac agacagataa aggcagaagc
     2881 atccaaacta aaacccataa ccaacctttt tccggaaatg gaaaatgcac aggaaaatat
     2941 agaggaactc caaactatgg gaatacaaaa taaagatatc cgacaacttc ttattggaaa
     3001 ggaaatatac tacactggaa acctttacga taaagacaag agaaaatcat accaagttaa
     3061 aaacgtgaaa atagatatct ccaaaagtca gaatggtatt actacaattt ggctaaataa
     3121 catccacttt aaaaactttc ttaaagaact ttggaataca ctacaaaaga ctttagataa
     3181 cggtaactgg ctacaccgat agaaagacac taaaaagaat tgcacttctc ttttttgaga
     3241 agtgcaattc aaattaaatc ccgttcattt tttaacgact aaaggtgcaa ttagctataa
     3301 aaccaaaaga aggagtagta ccttttcctt taaactccca tttaaaacct tgaccaggta
     3361 catttgcatc ccaatacgaa gtccaagtaa aagtgttact ccataacggt ccagttccac
     3421 taaaagcaac actttcaggt gtcgttatat tatcatccgg acttggttta gcattaggat
     3481 ttaatacttc aaagaaataa taattattct gtattgaagc attcaaatca acttgcatct
     3541 ttaaagtacg actggaatcg taaaaaactc cggtagtgct ttgagaacct ccattaatac
     3601 tcaaagaagg gagaacagcc ctcgtttccg ttacaacatc cctaaatact gaaaaatcaa
     3661 aattagcatc ctccttcacc tttttaacca aagtctttcc atttacagaa aaaacaacat
     3721 acgcggtatt aggatttgaa atagcacttg ccacttgagc atttaaaacc tgcaggttat
     3781 tcttatattt ctcaatactc aaaggactaa ctttttctaa atcctgccaa tccgaacctg
     3841 ttacataaga caacgccctc gttttcttat tctcattgaa atagtattca tttgtaatct
     3901 catttacatc aacaaaacga gataagacct ccatatcaga tacagaatca ctaaccacag
     3961 gttctattac ttcattatca ttacacgaac aaacacccaa aacagtcagt agactgatga
     4021 acataaacat tacttttttc ataaatcctc cttttttaaa gttaataaat aaaggtttcg
     4081 cccaaaaata taaaatttct cctatttttg cattgaatat taaatattta actatgaata
     4141 gaataataca tatctcttta cttatttcaa cagtatcact ttttcttatt tcaaacaccg
     4201 gatgtgttga aaaacaagga tactacaacc atggagaaga aagcataatc tcattaattt
     4261 gtgatattac atgggctggt aaaaaaacaa cagacgaaaa cggcagtgtc tggcaaggta
     4321 catacaaatt caataaaaac ggaacatata cccgtacaaa tatagaaatc gacaaacaag
     4381 gaaataaaaa agaagccaat atctacggac aatggtcatt cggtgatcca agtttcagta
     4441 ccatttattt tggtggagaa cattactggg acatagacga gctaactaaa aacaaatttt
     4501 ccttctatga cagaagtgga aaatttggcg atcctttcat gaatagagaa tacatagaac
     4561 tcacaccata ccaagaaaac aatacaacaa actaacaact acccactatt aggaaaaata
     4621 tcctcatcta aataaatatc ctgcaaaagt tcatccttcc gggtgacctt ataacaacac
     4681 aatgcctccc cagtttccat attataccaa tcagccacct tatctttcac agacatatac
     4741 caagtataac aaccatcttc aacattcaat ccctcaatat taagaaccaa atcctcatct
     4801 ctaaaatcat tcaacgcttc atctccgatc atggtgcgga actgtccgaa actactcatg
     4861 cgctgcatcc catgcatggc agtataaatc atatccaaag ccttataatt tatctgaatc
     4921 ttttttttac cgtcacgacc tttcgatttt gagacagaag cctttgaagc atacttaaaa
     4981 acctctatcg ctgcattttc aaaatcctct atcttcttat atttctgaaa acgggcatca
     5041 gcaaccgtac taccatgcaa tttcttccat tgggctacta catattttgc agcttccaat
     5101 gaatcaacaa taatatgaaa atgataatgg aacgaaaaca aatccttttt tactacatga
     5161 taggtacact ccttcttaat cacccccgaa ataccaagtg aacgaaactt gtgtttcgtc
     5221 cttataccat cagcccacat ccggttacat ctacgcatga aatctcgcaa tacccttgca
     5281 tcatcggtta cacaattttg caaggtaagc gtcacaaaat aaaactctct gccttccgaa
     5341 gccaaatact tcataaccgg aaggaacttc tttacatttt gtgccgtttg aatacgctga
     5401 cagataggac aaaaagcccg atgacaataa tgggaatgcg ccttacctcc ctgcacagta
     5461 taaacatttg aacacacatg ggcatccata tatgctttca tcagcttact atccggattc
     5521 ttttccgaaa gttttcgagt aaaattatgg gatatgattt ttactttagc ctttttttta
     5581 agactatcag aacc
//
