LOCUS       NZ_CP142414             2232 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KC25-20925 plasmid pKC25-20925_2.2kb,
            complete sequence.
ACCESSION   NZ_CP142414
VERSION     NZ_CP142414.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39432888
            Assembly: GCF_035928105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Klebsiella pneumoniae strain KC25-20925
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2024) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.76x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/22/2024 23:22:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,890
            CDSs (total)                      :: 5,767
            Genes (coding)                    :: 5,577
            CDSs (with protein)               :: 5,577
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 84 of 190
            Pseudo Genes (incomplete)         :: 117 of 190
            Pseudo Genes (internal stop)      :: 39 of 190
            Pseudo Genes (multiple problems)  :: 38 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142414.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KC25-20925"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKC25-20925_2.2kb"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.09 N 113.28 E"
                     /collection_date="2023"
                     /collected_by="Mingxiao Chen"
     gene            49..1017
                     /gene="mobV"
                     /locus_tag="VSU10_RS29445"
                     /old_locus_tag="VSU10_29455"
     CDS             49..1017
                     /gene="mobV"
                     /locus_tag="VSU10_RS29445"
                     /old_locus_tag="VSU10_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024818.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_053292550.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNADAERTPENYCS
                     VSQSADEAMGKLRELLPEKRRKDAVLAVEYVMTASPEWWNEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="mobV"
     gene            complement(1081..1515)
                     /locus_tag="VSU10_RS29450"
                     /old_locus_tag="VSU10_29460"
     CDS             complement(1081..1515)
                     /locus_tag="VSU10_RS29450"
                     /old_locus_tag="VSU10_29460"
                     /inference="COORDINATES: protein motif:HMM:NF017542.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_004193480.1"
                     /translation="MSNRITQIDTATGEEVGSFVAVIRPKQKSAFERHFTMNQDALKIL
                     AKTLTGEQFKVLMMLLADLDYENYIQIAQADIADALGMQKTNVSRSVKALIDLSVILQG
                     PKVGRSYSYRLNPQFGWKGTVSNHKKALKNGLSVIQGGRT"
                     /name="helix-turn-helix domain-containing protein"
     Typing          2..1014
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.45"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgcgatccc ccgactggcc ggatggacgc cggaacgagg gggtaagcat ggcgaattac
       61 gcgataatgc gctgcaaaaa gctgacggga atgggcagtg tggccagcgc gttgcagcac
      121 tgctacaggg agcgtgagac gccgaatgct gacgctgaac ggacaccaga aaactactgc
      181 tctgtgtctc agtcggcaga tgaggcgatg ggaaagctgc gggagctgtt gccggaaaaa
      241 cgccgtaaag atgcggtact ggctgttgaa tacgttatga cggcaagccc tgagtggtgg
      301 aacgaggcca caccgcggca gcaggcggag tttttcgccc gctctgagca atggctggag
      361 aagaaatacg ggaaggatcg tgttgtggcg gcggtggttc accgggacga ggccaccccg
      421 cacctgtctg cgttcgtggt gccattgacg caggatggac ggctgtccgc gaaagagttt
      481 attggcgggc ggtcaaagat gcgtgaagat cagagcacct acgctgaatc agtaaaaaaa
      541 ctgggtcttg agcgcggcat cgagggaagc cgcgccacgc atcagacagt tcagcactac
      601 tacgaatcga taaaccgtgg cacccgtagc caggtatcga tctcgccgga ggcgctggag
      661 ccgcgtgtat tgcggaaggg cattttcacc aaagacgtgg aagatcaggc agccatagcc
      721 aaacgtctaa gccacgcagt gaacgatggc tttgcaggaa ccatcgctat ggcctctcag
      781 agcgcccaga acgcaaaaag agcgcgtgag ctacagaaga ctatggatgc gcagcagaaa
      841 cgcctacaga gcgttacaga gccttttaag gggctttcca gagagcagat gaccgaaatc
      901 ctgatgatgg ctcagcggtt taagcagcaa aaccaggaaa aggaaaaaca gcagcgtatt
      961 gaacgtgaaa aacagcgaca gacgcgatcg cgtggcatgg gtggtatgga gcgttagttt
     1021 tgatgcgatg caggataggg ctaacgccgc aaagtgacgt catttgcggc attccagaga
     1081 tcaggttctg ccgccctgta taacgctgag gccgttttta agggcttttt tgtggttgct
     1141 gacagtacct ttccagccaa actgagggtt cagacggtaa ctgtaactgc gccctacttt
     1201 tgggccttga agaatcacgg ataggtcaat tagcgcctta accgaacggc ttacattggt
     1261 tttctgcata cctaaagcgt cggcaatatc ggcttgggca atttggatgt aattttcata
     1321 atcgagatca gctaacagca tcatcaagac tttgaattgc tcccctgtga gggtcttagc
     1381 aaggatcttt aaagcgtctt ggttcatagt gaaatgcctc tcaaaagctg acttctgttt
     1441 gggtcggata actgcgacaa aactacctac ctcttcccct gtagcagtgt ctatctgagt
     1501 aattcggtta ctcatttaag aggctctccc aaaaaaatag ttatcaataa tgataacaac
     1561 gttatcaata atgataacta tcgtcaagcg agagccgcgc cccacaaggg ctgagagaag
     1621 gctctcttct gtatcttata gcgagcgtag cgagtgccat acctaaagta cgattagtac
     1681 ttgagatgag tacgattagt acttgaggtg agtacgatta gtacttgagg tgagtacgat
     1741 tagtacttga ggtgagtacg attcttctct ctagcccttg gtgtgcgcgg gttcccgtat
     1801 ctatatctat atattaaggg gggaaataaa ggctgaaaaa cgccattttt aaacatcgaa
     1861 aaacgagctt ttttgagtct ggagcgtacg gatttctatg tccggggagt ggaaattccg
     1921 cttcctcgat cggacagact cgcggcgttg ccgctcccta atccgatctg cggcgagcgg
     1981 ttcctgctct ttcaaaagcg atatccagat cctgatttcg tcaagggcga gcggagcgag
     2041 tgcatttcac ccttgacgac gcacaaataa aacaggcttg gcagtggtgg gaggtgacgc
     2101 ggagcgacgc cttccacctg ccagaccgcc gttgattttg accttctggc agcttgctgc
     2161 ccgaacgatt ttgcgaagca aagtctagtg agtacactat catcctgata gtggcccgcc
     2221 ggaggcatcg ca
//
