LOCUS       NZ_CP142413             3597 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KC25-20925 plasmid pKC25-20925_3.6kb,
            complete sequence.
ACCESSION   NZ_CP142413
VERSION     NZ_CP142413.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39432888
            Assembly: GCF_035928105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Klebsiella pneumoniae strain KC25-20925
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2024) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.76x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/22/2024 23:22:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,890
            CDSs (total)                      :: 5,767
            Genes (coding)                    :: 5,577
            CDSs (with protein)               :: 5,577
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 84 of 190
            Pseudo Genes (incomplete)         :: 117 of 190
            Pseudo Genes (internal stop)      :: 39 of 190
            Pseudo Genes (multiple problems)  :: 38 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142413.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KC25-20925"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKC25-20925_3.6kb"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.09 N 113.28 E"
                     /collection_date="2023"
                     /collected_by="Mingxiao Chen"
     gene            1..195
                     /locus_tag="VSU10_RS29425"
                     /old_locus_tag="VSU10_29435"
     CDS             1..195
                     /locus_tag="VSU10_RS29425"
                     /old_locus_tag="VSU10_29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041642.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAESGTGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            691..849
                     /locus_tag="VSU10_RS29430"
                     /old_locus_tag="VSU10_29440"
     CDS             691..849
                     /locus_tag="VSU10_RS29430"
                     /old_locus_tag="VSU10_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071532985.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPKRHDLSPLAAATGNWNSRAMIM
                     RSVLQSS"
                     /name="replication initiation protein"
     gene            complement(1054..2571)
                     /locus_tag="VSU10_RS29435"
                     /old_locus_tag="VSU10_29445"
     CDS             complement(1054..2571)
                     /locus_tag="VSU10_RS29435"
                     /old_locus_tag="VSU10_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="WP_326913145.1"
                     /translation="MKKNIVRQVLESYGPCISSELAERIKRQNPSMSSDAIRKMISRST
                     DIGKLPFLRFSHNRRFIYLKDDFGSFKFWRALKKCMREANSAYSHAILSVINNGGYLKV
                     KDFGIVSGSPIKQAKHLSYESVLKNLLSAKILRSVYIDGVGDCVLINNNIANDISIFNM
                     ANCESFFDKPIFELIKAWLRNLGIVAFNQIKTKYDGENNPVVGSFEWDITAPSYVSPLA
                     EYSSDGLIPGFVVCDFALGFNRNEITTDAADTFIRKVQMTKSSRTNQRIMFVLFARRFE
                     KNAFNKLRSEGVLAITIANAFGNKVDESLARLSKVIQGTLSIERHPDELLQMVKDLESI
                     SGENGNLRGYIFELFVSSQICNFYGYGNIRINKEYKINGKHAEADVVLETNDDIYIVEC
                     KNVKTLPSMEVSLWMKTRVPIINSYYKSNNPDNKNIHHRLWVTGNIYPKDINRLDEFKC
                     NNKKIDVDYLFGQSLDDFFYQKKQVFNLYRKVISPDYKKGQIPDFDL"
                     /name="NERD domain-containing protein"
     gene            complement(3003..3326)
                     /gene="mobA"
                     /locus_tag="VSU10_RS29440"
                     /old_locus_tag="VSU10_29450"
     CDS             complement(3003..3326)
                     /gene="mobA"
                     /locus_tag="VSU10_RS29440"
                     /old_locus_tag="VSU10_29450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          complement(3336..3560)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.167"
                     /coverage="100.0"
                     /evalue="4.85e-75"
     Typing          239..759
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.714"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(3007..3326)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="6.09e-63"
ORIGIN
        1 atgaacaagc agcagcaaac cgtactcaac atggcgggat tcataaaaag ccagagcctg
       61 acgctgctcg aaaagctgga cgcactcgat gccgacgaac aggccgccat gtgtgagaag
      121 ctgcacgaac tcgcggaaga actccagaac agcatacaga cacgctttga agcggaaagt
      181 ggcaccgggg tataacagtg tatcctgctc cagacaggat gcagaacggt ataccggtgc
      241 gtaaggagaa aatgccacat aaggcgctct tccgcttcct cgctcactaa ctcgctacgc
      301 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa agacggtaat gcggttatcc
      361 acagaatcag gggataaagc caaaagaaac atgtgagcaa aaaacaagaa cccgaaaaag
      421 gccgcgccgg tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
      481 cgctcaagcc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      541 ggaagctccc tcgtgcgctc tcctgttccg accctgccgt ttaccggata cctgtccgcc
      601 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgttggta tctcggcccg
      661 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      721 tgcgccttat ccggtaacta tcgtcttgag tccaacccga aaagacacga cttatcgcca
      781 ctggcagcag ccactggtaa ctggaatagc agagcgatga taatgcggtc cgtactacag
      841 agttcttgaa gtgatgcccc aactacggct acacttaaag gacaggattt ggtatctgcg
      901 ctctgctgaa gccagttacc taggttaggt aatctgccag gattaactaa ccttcgaaaa
      961 accaccttgc cgggtggttt tttcgtttca tggaggaaga tacgcttttc aagccgtcat
     1021 cgtcaagaaa tgcagagact agaaaacgtc attctataag tcaaaatcgg gtatttgccc
     1081 ttttttataa tctggcgaaa tgactttcct atataaatta aacacttgtt ttttttggta
     1141 gaaaaaatca tccaaagact gaccaaataa ataatcaaca tcaatcttct tattattaca
     1201 tttaaactcg tccaatcggt ttatatcttt aggatatata ttccccgtaa cccacaaacg
     1261 atgatgtata tttttgttat cagggttatt tgatttgtag taggaattta taattggcac
     1321 tcttgttttc atccaaagac taacctccat cgaaggtaaa gtcttaacat ttttgcactc
     1381 aacgatataa atatcatcgt tagtttccaa tacaacatca gcttcggcat gtttaccgtt
     1441 tattttatat tctttattaa tacgaatatt tccataacca tagaaattac aaatttgaga
     1501 ggaaacaaaa agctcaaaaa tataccccct taaatttcca ttctctccag agatagactc
     1561 cagatctttt accatttgaa gtaactcatc aggatgtctt tcaatagata aagttccctg
     1621 aatcaccttc gatagtctgg ccaagctctc atcgacctta ttaccaaacg cattagctat
     1681 agttatagcc agaacccctt cactccttag cttgttgaag gcattcttct caaaacgtct
     1741 agcaaaaaga acaaacataa tcctctggtt tgtcctagag gacttggtca tctgaacttt
     1801 tcttatgaag gtatcagcag catcggtggt aatttcattt cgattaaacc ctagagcaaa
     1861 atcacagaca acaaaacctg ggatgagtcc atcgctggaa tattcagcaa gaggggacac
     1921 ataacttggc gctgttatat cccactcgaa agaaccaacc acaggattgt tctctccatc
     1981 atattttgtt tttatttgat taaaagccac aatacctaaa tttcgcaacc atgccttaat
     2041 cagttcaaat attggcttat caaaaaaaga ctcgcaattt gccatattaa agatgctgat
     2101 atcattagct atattattat ttatcaaaac acaatcacca acaccatcaa tatacacaga
     2161 tctaagtatt ttagctgaaa gcagattttt tagaacagat tcataactta aatgcttggc
     2221 ctgctttatc ggagagccag acacaatccc gaaatccttt accttcaagt atccgccatt
     2281 attaattacg gaaagtatag cgtgagaata cgccgaattt gcctcacgca tacatttttt
     2341 gagcgcccgc caaaatttga agctaccaaa gtcatctttt agataaatga atctcctgtt
     2401 atgactaaat cttaaaaatg gcaacttacc aatatcagtg cttcgagaga tcattttacg
     2461 tattgcatca gatgacatag aaggattctg acgcttaatc ctctcagcaa gttcagaaga
     2521 tatgcacgga ccataactct caagcacttg tctaacaatg ttttttttca taaattcatc
     2581 ttatttttgt cacactacgc tatttataaa attacactac gtgtaatatc tatagatccc
     2641 aaaaaaaaca caaagaaaaa aaatcataaa aatcaaatat ttaacaaaag caacatatca
     2701 caaaaaacac acctgcgaca tttatcctta aaaatcaaga tgttacgttt gtgacatttc
     2761 atcatcatat atcaaccgcc ctatttacca taatccctta attgtacgca ccgcagaaac
     2821 gcgctgaacg ggattctgcc gcagacaccg caaaaagaga tgtgttacca agcgaaaacc
     2881 ggaaaaatgc gtcaggatcg ttttcagctc gctgcatagc tatgcatgaa agcgaatggt
     2941 gatcactttg ggagcttacg gtgttcatac cgtctgtttt cgacagtttt ctctccggga
     3001 agctaatctg ccataagcct ggataacagg gcacggtgat actccgtaat agcgatcagc
     3061 acctccgcat actccctgtc cccgacacgc ttgccgtcga taaagagttt tttctgtaac
     3121 gcccccagtc tcataacttc tttcagtacc cgatcatcag tcagtgagtt aactttctta
     3181 ccgagagctg ccgcccgtaa aaaaccggaa acagtcttgc cgcattcagc agcccttttt
     3241 ctgatttcct ggtcttcttc tgccgtcaga cgaacagggc gactgatagc ccgcctgcgc
     3301 gtattacttc cacttcgttt tgtcataaaa catggctccg tatctgacac ggggtgtcgg
     3361 ggcgaagccc tgaccagggt aattgtaata gcgtgcatgc atgcgcggta taacaattgc
     3421 acatcctgtc ctgtctgcaa gttcgaatta ccaacaaagc actgctgttt tttggcagtg
     3481 acagtgttgt ctgactgaaa tgtccgtcgg cagaataccg gcggtaagac ggtcgaaaag
     3541 tggtacgcga tataatcaca cgacgcagag gacagacaga acggagaaaa cagaaag
//
