LOCUS       NZ_CP142412             4018 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KC25-20925 plasmid pKC25-20925_4kb,
            complete sequence.
ACCESSION   NZ_CP142412
VERSION     NZ_CP142412.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39432888
            Assembly: GCF_035928105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Klebsiella pneumoniae strain KC25-20925
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2024) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.76x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/22/2024 23:22:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,890
            CDSs (total)                      :: 5,767
            Genes (coding)                    :: 5,577
            CDSs (with protein)               :: 5,577
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 84 of 190
            Pseudo Genes (incomplete)         :: 117 of 190
            Pseudo Genes (internal stop)      :: 39 of 190
            Pseudo Genes (multiple problems)  :: 38 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142412.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KC25-20925"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKC25-20925_4kb"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.09 N 113.28 E"
                     /collection_date="2023"
                     /collected_by="Mingxiao Chen"
     gene            1..987
                     /locus_tag="VSU10_RS29405"
                     /old_locus_tag="VSU10_29415"
     CDS             1..987
                     /locus_tag="VSU10_RS29405"
                     /old_locus_tag="VSU10_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_326913144.1"
                     /translation="MENPELQNLTDYSPSDAPWDAHRSASDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLSRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHARWVELWRECLRVDYDPSVDVRAVKSRKPKDGE
                     SLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLKL
                     DQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     gene            1397..1726
                     /locus_tag="VSU10_RS29410"
                     /old_locus_tag="VSU10_29420"
     CDS             1397..1726
                     /locus_tag="VSU10_RS29410"
                     /old_locus_tag="VSU10_29420"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024191011.1"
                     /translation="MVSFIAQGCAPNPANASRYQIKNLSAFTVPEFVKQKIAGSRAFGV
                     GSDFVIDDGTGKKHWGWWLVSRSEANHLQDGFVVHKISGKACECFVGKTLSGLLFLPPE
                     NQLRF"
                     /name="hypothetical protein"
     gene            2010..2996
                     /locus_tag="VSU10_RS29415"
                     /old_locus_tag="VSU10_29425"
     CDS             2010..2996
                     /locus_tag="VSU10_RS29415"
                     /old_locus_tag="VSU10_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_326913144.1"
                     /translation="MENPELQNLTDYSPSDAPWDAHRSASDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLSRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHARWVELWRECLRVDYDPSVDVRAVKSRKPKDGE
                     SLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLKL
                     DQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
                     /name="protein rep"
     gene            3406..3735
                     /locus_tag="VSU10_RS29420"
                     /old_locus_tag="VSU10_29430"
     CDS             3406..3735
                     /locus_tag="VSU10_RS29420"
                     /old_locus_tag="VSU10_29430"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_024191011.1"
                     /translation="MVSFIAQGCAPNPANASRYQIKNLSAFTVPEFVKQKIAGSRAFGV
                     GSDFVIDDGTGKKHWGWWLVSRSEANHLQDGFVVHKISGKACECFVGKTLSGLLFLPPE
                     NQLRF"
                     /name="hypothetical protein"
     Typing          990..1071
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.181"
                     /coverage="90.0"
                     /evalue="7.3e-29"
     Typing          2999..3080
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.181"
                     /coverage="90.0"
                     /evalue="7.3e-29"
     Typing          complement(714..905)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
     Typing          complement(2723..2914)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.964"
                     /coverage="100.0"
                     /evalue="6.14e-93"
ORIGIN
        1 atggaaaatc ctgaactcca gaacctcacg gactattccc cctcagatgc gccatgggac
       61 gctcacagaa gcgcttctga cgacgtggga gggatttacc tgcttgcagc tgaatatgag
      121 cgttacgggg cgcgtatggc ctcctgtggc ggtttactgc gttttggatg gtctaccctc
      181 aaggaaaccg gagaaacgcg actgaggctg cgtgaggcgc atttctgccg tgtccggcac
      241 tgtcctgtct gccagtggag gcgctccctg atgtggcaag cccgttttta tcagtctttg
      301 ccgcgtattg tggctgacta tccagacgca cgatggatgt ttctgaccct gaccgtgcgc
      361 aactgtgcga taggggagct gggcgagaca ctgagccgaa tgaacatggc cttccagcga
      421 ctgaaggatc gcaaagagtt tcgacctgtt cagggctgga tccgcacgac ggaggttact
      481 cgaggcagcg acggtagcgc acacccgcac ttccataccc tgatgatggt gccgccctcg
      541 atgttcacgc gtgactatgt caaacatgcg cgctgggttg aattgtggcg ggagtgtctg
      601 cgggtagatt acgacccgag tgttgatgtc cgggcagtga aatcccgcaa gccgaaggac
      661 ggcgaatcgc tggcctgcgc gaccgccgaa ctggtgcgcg gtgcggtggc cgaaaccctg
      721 aaatattcca cgaagccggc tgacatggtg gccgatcctg aatggttcct cgaactgact
      781 cgccagacgc acaaacgccg ctttgtggcg accggcggcg cactcaaaga cgtgctgaaa
      841 ctcgatcagg aaaccgacgc cgacatggtg atcggggatg atatttccga gggcgacgat
      901 gacggttcac gcatcgcgtt cgagtggaaa accgaggtca gaaagtaccg tcgctcaccg
      961 acgaaagata aaactgaatc aggctagcgg ggtgtcgggg tgcacccctg accagatggc
     1021 aattgtgata gcgtcgcgtg tgacggtatt acaattgcac atcctgtccc gaaaattctc
     1081 gccgtatctc ggttttttga gcgtagcagg ccgcaggaca aacgaaacca atgcaggaca
     1141 aacgaaacca atgcaggata gacaatcatg gtcagaacgt taacacgttg cccagattgt
     1201 ccgcctgccg caggctctgt ttttaatttt cggtgaaaac aaaaattaaa attctggatt
     1261 ttggaaagag tgtgttttta aaaacacgcg tttttgattt ttgaaaagag cctgcggcag
     1321 gcacaggttg ataatccgat gacaaatgcc caatttgaaa attggtaaca attgacatcg
     1381 gtttgtctat cctgcattgg tttcgttcat cgctcagggc tgcgccccga accccgccaa
     1441 cgcctcacgc tatcagatta aaaatctgtc agcgttcacc gttcccgaat tcgtcaaaca
     1501 gaaaatcgcg gggtcccgcg cctttggcgt gggttccgat tttgtgattg acgacggcac
     1561 gggaaaaaaa cactggggat ggtggttggt gtcacgcagt gaggccaacc acctgcaaga
     1621 cggttttgtt gttcacaaaa tcagtggaaa agcctgtgag tgttttgtcg ggaaaacgct
     1681 ttcggggttg ttgtttttgc cgccagaaaa tcagctcaga ttttgagcaa atctcgcgcg
     1741 cgatccttgt atttatactt aagggataac tctggggata aaaaatagct acaggccttg
     1801 tgtggcaagg ctttgcggct tatttgcgtt gtagaaggtt ttagaagtat tctgtcgaaa
     1861 gtggtcgcat taaaagcgac ccccgcaact gcgctaacag ttcgggggtg tgaccacctg
     1921 taggaattcg aattggtgac acgacaacac aggtgatatg gcaatgataa cacgtcagat
     1981 gagccatcgc ccacaatcgg gtggtgtgca tggaaaatcc tgaactccag aacctcacgg
     2041 actattcccc ctcagatgcg ccatgggacg ctcacagaag cgcttctgac gacgtgggag
     2101 ggatttacct gcttgcagct gaatatgagc gttacggggc gcgtatggcc tcctgtggcg
     2161 gtttactgcg ttttggatgg tctaccctca aggaaaccgg agaaacgcga ctgaggctgc
     2221 gtgaggcgca tttctgccgt gtccggcact gtcctgtctg ccagtggagg cgctccctga
     2281 tgtggcaagc ccgtttttat cagtctttgc cgcgtattgt ggctgactat ccagacgcac
     2341 gatggatgtt tctgaccctg accgtgcgca actgtgcgat aggggagctg ggcgagacac
     2401 tgagccgaat gaacatggcc ttccagcgac tgaaggatcg caaagagttt cgacctgttc
     2461 agggctggat ccgcacgacg gaggttactc gaggcagcga cggtagcgca cacccgcact
     2521 tccataccct gatgatggtg ccgccctcga tgttcacgcg tgactatgtc aaacatgcgc
     2581 gctgggttga attgtggcgg gagtgtctgc gggtagatta cgacccgagt gttgatgtcc
     2641 gggcagtgaa atcccgcaag ccgaaggacg gcgaatcgct ggcctgcgcg accgccgaac
     2701 tggtgcgcgg tgcggtggcc gaaaccctga aatattccac gaagccggct gacatggtgg
     2761 ccgatcctga atggttcctc gaactgactc gccagacgca caaacgccgc tttgtggcga
     2821 ccggcggcgc actcaaagac gtgctgaaac tcgatcagga aaccgacgcc gacatggtga
     2881 tcggggatga tatttccgag ggcgacgatg acggttcacg catcgcgttc gagtggaaaa
     2941 ccgaggtcag aaagtaccgt cgctcaccga cgaaagataa aactgaatca ggctagcggg
     3001 gtgtcggggt gcacccctga ccagatggca attgtgatag cgtcgcgtgt gacggtatta
     3061 caattgcaca tcctgtcccg aaaattctcg ccgtatctcg gttttttgag cgtagcaggc
     3121 cgcaggacaa acgaaaccaa tgcaggacaa acgaaaccaa tgcaggatag acaatcatgg
     3181 tcagaacgtt aacacgttgc ccagattgtc cgcctgccgc aggctctgtt tttaattttc
     3241 ggtgaaaaca aaaattaaaa ttctggattt tggaaagagt gtgtttttaa aaacacgcgt
     3301 ttttgatttt tgaaaagagc ctgcggcagg cacaggttga taatccgatg acaaatgccc
     3361 aatttgaaaa ttggtaacaa ttgacatcgg tttgtctatc ctgcattggt ttcgttcatc
     3421 gctcagggct gcgccccgaa ccccgccaac gcctcacgct atcagattaa aaatctgtca
     3481 gcgttcaccg ttcccgaatt cgtcaaacag aaaatcgcgg ggtcccgcgc ctttggcgtg
     3541 ggttccgatt ttgtgattga cgacggcacg ggaaaaaaac actggggatg gtggttggtg
     3601 tcacgcagtg aggccaacca cctgcaagac ggttttgttg ttcacaaaat cagtggaaaa
     3661 gcctgtgagt gttttgtcgg gaaaacgctt tcggggttgt tgtttttgcc gccagaaaat
     3721 cagctcagat tttgagcaaa tctcgcgcgc gatccttgta tttatactta agggataact
     3781 ctggggataa aaaatagcta caggccttgt gtggcaaggc tttgcggctt atttgcgttg
     3841 tagaaggttt tagaagtatt ctgtcgaaag tggtcgcatt aaaagcgacc cccgcaactg
     3901 cgctaacagt tcgggggtgt gaccacctgt aggaattcga attggtgaca cgacaacaca
     3961 ggtgatatgg caatgataac acgtcagatg agccatcgcc cacaatcggg tggtgtgc
//
