LOCUS       NZ_CP142411             6477 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KC25-20925 plasmid pKC25-20925_6.5kb,
            complete sequence.
ACCESSION   NZ_CP142411
VERSION     NZ_CP142411.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39432888
            Assembly: GCF_035928105.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 6477)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Klebsiella pneumoniae strain KC25-20925
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6477)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-JAN-2024) Zhuhai Hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 56.76x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/22/2024 23:22:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,890
            CDSs (total)                      :: 5,767
            Genes (coding)                    :: 5,577
            CDSs (with protein)               :: 5,577
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 190
            CDSs (without protein)            :: 190
            Pseudo Genes (ambiguous residues) :: 0 of 190
            Pseudo Genes (frameshifted)       :: 84 of 190
            Pseudo Genes (incomplete)         :: 117 of 190
            Pseudo Genes (internal stop)      :: 39 of 190
            Pseudo Genes (multiple problems)  :: 38 of 190
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142411.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6477
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KC25-20925"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKC25-20925_6.5kb"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.09 N 113.28 E"
                     /collection_date="2023"
                     /collected_by="Mingxiao Chen"
     gene            43..897
                     /gene="estX"
                     /locus_tag="VSU10_RS29360"
                     /old_locus_tag="VSU10_29370"
     CDS             43..897
                     /gene="estX"
                     /locus_tag="VSU10_RS29360"
                     /old_locus_tag="VSU10_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold putative hydrolase EstX"
                     /protein_id="WP_094315607.1"
                     /translation="MKEKVVVDKAISLHTESFGDPAHEPIILIMGAMASAVWWPDEFCS
                     QLAKMGRYVIRYDHRDTGKSTSYEPGQAPYSVEELADDVVRVIDGYGLEAAHLVGMSLG
                     GFLSQLVALKYPKRVKSLTLIASERLADADPDMPAFDPAIIEYHQRAESLDWSDRDAVV
                     AYQVGAWRIISGTAHAFDAEKIQNIAELDFDRTPNILTTFNHTTSGGGERSLERWLGRL
                     NEIAVPTLIIHGTEDPVLPYVHGLALKDAIRGSKMLTLEGTGHELHHEDWPRIIQAIKG
                     QTS"
                     /name="estX"
     gene            complement(1567..1851)
                     /locus_tag="VSU10_RS29365"
                     /old_locus_tag="VSU10_29375"
     CDS             complement(1567..1851)
                     /locus_tag="VSU10_RS29365"
                     /old_locus_tag="VSU10_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            2051..>2692
                     /locus_tag="VSU10_RS29370"
                     /old_locus_tag="VSU10_29380"
                     /pseudo=""
     CDS             2051..>2692
                     /locus_tag="VSU10_RS29370"
                     /old_locus_tag="VSU10_29380"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            2799..3212
                     /locus_tag="VSU10_RS29375"
                     /old_locus_tag="VSU10_29385"
     CDS             2799..3212
                     /locus_tag="VSU10_RS29375"
                     /old_locus_tag="VSU10_29385"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            3311..4180
                     /locus_tag="VSU10_RS29380"
                     /old_locus_tag="VSU10_29390"
     CDS             3311..4180
                     /locus_tag="VSU10_RS29380"
                     /old_locus_tag="VSU10_29390"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            4241..4453
                     /locus_tag="VSU10_RS29385"
                     /old_locus_tag="VSU10_29395"
     CDS             4241..4453
                     /locus_tag="VSU10_RS29385"
                     /old_locus_tag="VSU10_29395"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            4455..4661
                     /gene="urfF"
                     /locus_tag="VSU10_RS29390"
                     /old_locus_tag="VSU10_29400"
     CDS             4455..4661
                     /gene="urfF"
                     /locus_tag="VSU10_RS29390"
                     /old_locus_tag="VSU10_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            4691..5530
                     /locus_tag="VSU10_RS29395"
                     /old_locus_tag="VSU10_29405"
     CDS             4691..5530
                     /locus_tag="VSU10_RS29395"
                     /old_locus_tag="VSU10_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            5517..6368
                     /gene="repC"
                     /locus_tag="VSU10_RS29400"
                     /old_locus_tag="VSU10_29410"
     CDS             5517..6368
                     /gene="repC"
                     /locus_tag="VSU10_RS29400"
                     /old_locus_tag="VSU10_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     Typing          1806..1962
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.38e-77"
     Typing          4709..5530
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2052..4177
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaataaaa tgttgtacgt aaaactcgaa ggcaatttta gtatgaaaga aaaggtagtt
       61 gttgataaag cgatttcact ccataccgaa tcattcggcg acccggccca tgaacccatt
      121 atcctgatca tgggggcaat ggcgtctgcg gtgtggtggc ctgatgagtt ttgctcccaa
      181 ctagccaaaa tgggtcgcta tgtgatccgg tacgaccacc gtgataccgg gaaatcaaca
      241 agctatgagc caggtcaagc tccatattcc gttgaagaat tagcagatga tgtggttcgc
      301 gtcattgatg gttatggtct ggaagctgct catttagtcg gcatgtcttt ggggggattt
      361 ctttcccagc ttgtagctct caagtatccg aaacgtgtga agagcttgac gctgattgct
      421 tcagaacggc ttgcagatgc agatcctgat atgcccgctt ttgatcctgc catcattgag
      481 tatcaccaac gggcggaatc gctggattgg tctgatagag atgctgtcgt cgcgtatcag
      541 gtcggagcgt ggcgaatcat ctcaggtact gcgcatgctt ttgacgctga gaagatacaa
      601 aatatcgctg agttagattt tgatcgcact ccgaatatcc tgacaacatt caaccacact
      661 acttcaggtg gtggcgagag atcgctcgag agatggctcg ggagattaaa tgagatagct
      721 gtaccaactt tgatcattca cggcacggag gatcctgtac ttccttatgt gcatgggttg
      781 gcactgaaag atgcgattcg tggttcaaaa atgctgacac tcgaaggcac gggacatgag
      841 ttgcatcatg aagactggcc gaggattatc caggcgatta aggggcaaac gtcatagcta
      901 tcggttgcac cgtacaacaa gttgctgcac cgtaaaaatt actcgctgcg ctcgcaattt
      961 tccggtgagc aaggcgttaa acatcatgag ggaagcggtg atcgctgagg catgttccgc
     1021 gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc ttttaccctc
     1081 agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc ccgtactaac
     1141 tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac gaacccctgc
     1201 aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg cggggtgttg
     1261 gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc agcgcccttg
     1321 tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct ggtctattcg
     1381 ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg ggcaaagccc
     1441 gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc gctttagcgg
     1501 cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc tgtctcggtc
     1561 gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg cgcggtcgtg
     1621 gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc actcgctgct
     1681 gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt cgttcttgcg
     1741 ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc gcgctcgttg
     1801 ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca tcttgacacc
     1861 ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc aatcccgacc
     1921 ctactttgta gggggagggc gcacttaccg gtttctcttc gagaaactgg cctaacggcc
     1981 acccttcggg cggtgcgctc tccgagggcc attgcatgga gccgaaaagc aaaagcaaca
     2041 gcgaggcagc atggcgattt atcaccttac ggcgaaaacc ggcagcaggt cgggcggcca
     2101 atcggccagg gccaaggccg actacatcca gcgcgaaggc aagtatgccc gcgacatgga
     2161 tgaagtcttg cacgccgaat ccgggcacat gccggagttc gtcgagcggc ccgccgacta
     2221 ctgggatgct gccgacctgt atgaacgcgc caatgggcgg ctgttcaagg aggtcgaatt
     2281 tgccctgccg gtcgagctga ccctcgacca gcagaaggcg ctggcgtccg agttcgccca
     2341 gcacctgact ggtgccgagc gcctgccgta tacgctggcc atccatgccg gtggcggcga
     2401 gaacccgcac tgccacctga tgatctccga gcggatcaat gacggcatcg agcggcccgc
     2461 cgctcagtgg ttcaagcggt acaacggcaa gaccccggag aagggcgggg cacagaagac
     2521 cgaagcgctc aagcccaagg catggcttga gcagacccgc gaggcatggg ccgaccatgc
     2581 caaccgggca ttagagcggg ctggccacga cgcccgcatt gaccacagaa cacttgaggc
     2641 gcagggcatc gagcgcctgc ccggtgttca cctggggccg aacgtggtgg agatggaagg
     2701 ccggggcatc cgcaccgacc gggcagacgt ggccctgaac atcgacaccg ccaacgccca
     2761 gatcatcgac ttacaggaat accgggaggc aatagaccat gaacgcaatc gacagagtga
     2821 agaaatccag aggcatcaac gagttagcgg agcagatcga accgctggcc cagagcatgg
     2881 cgacactggc cgacgaagcc cggcaggtca tgagccagac ccagcaggcc agcgaggcgc
     2941 aggcggcgga gtggctgaaa gcccagcgcc agacaggggc ggcatgggtg gagctggcca
     3001 aagagttgcg ggaggtagcc gccgaggtga gcagcgccgc gcagagcgcc cggagcgcgt
     3061 cgcgggggtg gcactggaag ctatggctaa ccgtgatgct ggcttccatg atgcctacgg
     3121 tggtgctgct gatcgcatcg ttgctcttgc tcgacctgac gccactgaca accgaggacg
     3181 gctcgatctg gctgcgcttg gtggcccgat gaagaacgac aggactttgc aggccatagg
     3241 ccgacagctc aaggccatgg gctgtgagcg cttcgatatc ggcgtcaggg acgccaccac
     3301 cggccagatg atgaaccggg aatggtcagc cgccgaagtg ctccagaaca cgccatggct
     3361 caagcggatg aatgcccagg gcaatgacgt gtatatcagg cccgccgagc aggagcggca
     3421 tggtctggtg ctggtggacg acctcagcga gtttgacctg gatgacatga aagccgaggg
     3481 ccgggagcct gccctggtag tggaaaccag cccgaagaac tatcaggcat gggtcaaggt
     3541 ggccgacgcc gcaggcggtg aacttcgggg gcagattgcc cggacgctgg ccagcgagta
     3601 cgacgccgac ccggccagcg ccgacagccg ccactatggc cgcttggcgg gcttcaccaa
     3661 ccgcaaggac aagcacacca cccgcgccgg ttatcagccg tgggtgctgc tgcgtgaatc
     3721 caagggcaag accgccaccg ctggcccggc gctggtgcag caggctggcc agcagatcga
     3781 gcaggcccag cggcagcagg agaaggcccg caggctggcc agcctcgaac tgcccgagcg
     3841 gcagcttagc cgccaccggc gcacggcgct ggacgagtac cgcagcgaga tggccgggct
     3901 ggtcaagcgc ttcggtgatg acctcagcaa gtgcgacttt atcgccgcgc agaagctggc
     3961 cagccggggc cgcagtgccg aggaaatcgg caaggccatg gccgaggcca gcccagcgct
     4021 ggcagagcgc aagcccggcc acgaagcgga ttacatcgag cgcaccgtca gcaaggtcat
     4081 gggtctgccc agcgtccagc ttgcgcgggc cgagctggca cgggcaccgg caccccgcca
     4141 gcgaggcatg gacaggggcg ggccagattt cagcatgtag tgcttgcgtt ggtactcacg
     4201 cctgttatac tatgagtact cacgcacaga agggggtttt atggaatacg aaaaaagcgc
     4261 ttcagggtcg gtctacctga tcaaaagtga caagggctat tggttgcccg gtggctttgg
     4321 ttatacgtca aacaaggccg aggctggccg cttttcagtc gctgatatgg ccagccttaa
     4381 ccttgacggc tgcaccttgt ccttgttccg cgaagacaag cctttcggcc ccggcaagtt
     4441 tctcggtgac tgatatgaaa gaccaaaagg acaagcagac cggcgacctg ctggccagcc
     4501 ctgacgctgt acgccaagcg cgatatgccg agcgcatgaa ggccaaaggg atgcgtcagc
     4561 gcaagttctg gctgaccgac gacgaatacg aggcgctgcg cgagtgcctg gaagaactca
     4621 gagcggcgca gggcgggggt agtgaccccg ccagcgccta accaccaact gcctgcaaag
     4681 gaggcaatca atggctaccc ataagcctat caatattctg gaggcgttcg cagcagcgcc
     4741 gccaccgctg gactacgttt tgcccaacat ggtggccggt acggtcgggg cgctggtgtc
     4801 gcccggtggt gccggtaaat ccatgctggc cctgcaactg gccgcacaga ttgcaggcgg
     4861 gccggatctg ctggaggtgg gcgaactgcc caccggcccg gtgatctacc tgcccgccga
     4921 agacccgccc accgccattc atcaccgcct gcacgccctt ggggcgcacc tcagcgccga
     4981 ggaacggcaa gccgtggctg acggcctgct gatccagccg ctgatcggca gcctgcccaa
     5041 catcatggcc ccggagtggt tcgacggcct caagcgcgcc gccgagggcc gccgcctgat
     5101 ggtgctggac acgctgcgcc ggttccacat cgaggaagaa aacgccagcg gccccatggc
     5161 ccaggtcatc ggtcgcatgg aggccatcgc cgccgatacc gggtgctcta tcgtgttcct
     5221 gcaccatgcc agcaagggcg cggccatgat gggcgcaggc gaccagcagc aggccagccg
     5281 gggcagctcg gtactggtcg ataacatccg ctggcagtcc tacctgtcga gcatgaccag
     5341 cgccgaggcc gaggaatggg gtgtggacga cgaccagcgc cggttcttcg tccgcttcgg
     5401 tgtgagcaag gccaactatg gcgcaccgtt cgctgatcgg tggttcaggc ggcatgacgg
     5461 cggggtgctc aagcccgccg tgctggagag gcagcgcaag agcaaggggg tgccccgtgg
     5521 tgaagcctaa gaacaagcac agcctcagcc acgtccggca cgacccggcg cactgtctgg
     5581 cccccggcct gttccgtgcc ctcaagcggg gcgagcgcaa gcgcagcaag ctggacgtga
     5641 cgtatgacta cggcgacggc aagcggatcg agttcagcgg cccggagccg ctgggcgctg
     5701 atgatctgcg catcctgcaa gggctggtgg ccatggctgg gcctaatggc ctagtgcttg
     5761 gcccggaacc caagaccgaa ggcggacggc agctccggct gttcctggaa cccaagtggg
     5821 aggccgtcac cgctgatgcc atggtggtca aaggtagcta tcgggcgctg gcaaaggaaa
     5881 tcggggcaga ggtcgatagt ggtggggcgc tcaagcacat acaggactgc atcgagcgcc
     5941 tttggaaggt atccatcatc gcccagaatg gccgcaagcg gcaggggttt cggctgctgt
     6001 cggagtacgc cagcgacgag gcggacgggc gcctgtacgt ggccctgaac cccttgatcg
     6061 cgcaggccgt catgggtggc ggccagcatg tgcgcatcag catggacgag gtgcgggcgc
     6121 tggacagcga aaccgcccgc ctgctgcacc agcggctgtg tggctggatc gaccccggca
     6181 aaaccggcaa ggcttccata gataccttgt gcggctatgt ctggccgtca gaggccagtg
     6241 gttcgaccat gcgcaagcgc cgccagcggg tgcgcgaggc gttgccggag ctggtcgcgc
     6301 tgggctggac ggtaaccgag ttcgcggcgg gcaagtacga catcacccgg cccaaggcgg
     6361 caggctgacc ccccccactc tattgtaaac aagacatttt tatctttata aaacaattgg
     6421 ttggggatgt ttgagcgcaa ttaacaggca gccaaaactg gcaaaatgag catgcct
//
