LOCUS       NZ_CP142051             3133 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Escherichia coli strain L9 plasmid pL9-7, complete sequence.
ACCESSION   NZ_CP142051
VERSION     NZ_CP142051.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39307979
            Assembly: GCF_035666355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Zhang,L., Tao,M., Li,M. and Liu,J.
  TITLE     Effect of colistin on Escherichia coli biofilm
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Zhang,L., Tao,M., Li,M. and Liu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2024) College of Veterinary Medicine, Henan
            Agricultural University, Longzihu District, Zhengzhou, Henan 450046,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-JUN-2023
            Assembly Method :: A5-miseeq v. V20152522; CANU v. v1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina TruSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 07:58:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,001
            CDSs (total)                      :: 4,880
            Genes (coding)                    :: 4,623
            CDSs (with protein)               :: 4,623
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 70 of 257
            Pseudo Genes (incomplete)         :: 192 of 257
            Pseudo Genes (internal stop)      :: 51 of 257
            Pseudo Genes (multiple problems)  :: 51 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142051.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="L9"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pL9-7"
                     /geo_loc_name="China:Henan"
                     /collection_date="2023-06-13"
     gene            1..753
                     /locus_tag="VPJ62_RS24975"
                     /old_locus_tag="VPJ62_24975"
     CDS             1..753
                     /locus_tag="VPJ62_RS24975"
                     /old_locus_tag="VPJ62_24975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016249639.1"
                     /translation="MDTTSLISSISVPRLSFYETFLGCATDQEKVGAYVAYQDLSGDFF
                     CLVQMIEVALRNAINNTIKANHGPAWYDSIPQTKKSQDLVLNAKQKALDECGVRYTDDD
                     VICRLTFGFWVYMLDAPYRDTQRPCYIWTPENKAKTFPHAKNPLGGGDMKVKALFDEFH
                     KVLLFRNRLFHHEPIWKKHHCKSHSQAINNMLKEFNFLMNALSIVSNEKKELIEFIGHD
                     RRFYERCTIEYVMGIIGRIKCRELKVAG"
                     /name="hypothetical protein"
     gene            complement(778..921)
                     /locus_tag="VPJ62_RS24980"
                     /old_locus_tag="VPJ62_24980"
     CDS             complement(778..921)
                     /locus_tag="VPJ62_RS24980"
                     /old_locus_tag="VPJ62_24980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152918943.1"
                     /translation="MNTKVLFVKKALKSITCAILSLLPALSRAVTELRSLSAARLKAGK
                     SF"
                     /name="hypothetical protein"
     gene            complement(1283..1396)
                     /locus_tag="VPJ62_RS24985"
                     /old_locus_tag="VPJ62_24985"
     CDS             complement(1283..1396)
                     /locus_tag="VPJ62_RS24985"
                     /old_locus_tag="VPJ62_24985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071790583.1"
                     /translation="MHEPPVQSDYCALSGNYRLESNPERHDLSPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(1873..2067)
                     /locus_tag="VPJ62_RS24990"
                     /old_locus_tag="VPJ62_24990"
     CDS             complement(1873..2067)
                     /locus_tag="VPJ62_RS24990"
                     /old_locus_tag="VPJ62_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_025492124.1"
                     /translation="MNKQQQTALNMAKFIKSQSLTLLEKLDSLDADAQAAMCEKLHELA
                     EELQNSIQTRFESESGTGE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2352..2675
                     /gene="mobA"
                     /locus_tag="VPJ62_RS24995"
                     /old_locus_tag="VPJ62_24995"
     CDS             2352..2675
                     /gene="mobA"
                     /locus_tag="VPJ62_RS24995"
                     /old_locus_tag="VPJ62_24995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          2106..2343
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.167"
                     /coverage="99.0"
                     /evalue="2.15e-118"
     Typing          complement(1126..1575)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.494"
                     /coverage="90.0"
                     /evalue="1.02e-154"
     Typing          2353..2672
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.32e-62"
ORIGIN
        1 atggatacaa caagtttaat tagctcaatt tctgtaccaa gattatcttt ctatgaaaca
       61 tttcttgggt gcgctactga tcaagaaaag gttggggcat acgttgccta ccaagatctg
      121 tctggcgatt ttttttgtct tgtccagatg attgaggttg ctctgcgtaa tgcgataaac
      181 aatacaatca aagctaatca tgggcctgct tggtacgact ctatacctca aacaaagaaa
      241 tctcaggatc ttgttttaaa tgcgaaacaa aaagcgctag atgagtgtgg cgtaaggtat
      301 acggatgatg atgttatttg ccgtctaacc tttggctttt gggtttatat gttagatgcc
      361 ccatataggg atacgcaaag gccgtgctac atctggactc cagagaacaa ggcaaaaaca
      421 tttcctcatg cgaagaatcc tttgggtggg ggggatatga aggttaaagc gttgtttgac
      481 gagtttcata aagttctttt gtttaggaat cgactttttc accatgagcc tatttggaaa
      541 aaacaccact gcaagtcgca tagtcaagcc ataaataata tgttgaaaga gttcaatttt
      601 ttaatgaatg ccttgagtat cgtctctaac gagaaaaagg aacttattga gtttattggg
      661 cacgataggc gcttttatga acgctgcacg atagagtatg ttatggggat tatagggagg
      721 attaagtgca gggagcttaa ggtagccggt tgacttttaa ggaaaaatgt gatacaatca
      781 aaagctctta ccggctttaa gtcgcgcagc cgaaaggcta cgtaactctg ttactgcgcg
      841 acttaaagcc ggtaagagcg aaagaattgc gcaagtaatt gatttcaagg cttttttcac
      901 aaaaagaact ttagtattca ttttcataat cctctatttc ttatgccgtt ctaatggcag
      961 cgttgacttt ttattccatc tatttgaatg gttaattata ccatatctga catcaaatct
     1021 taaggataaa tagctgattc acatcagttt ttccattaat ccacaaactt ttataattta
     1081 attgcggatt tccttgagtt catatcgcgc ccatcgtaat cttaaaaacg aaaaaaccac
     1141 ccggcaaggt ggtttttcga aggttcagca accggcaagt ttctgaacct cggtaactgg
     1201 cttaagagga gcgcagtcac caaaacttgt tcttccagtt tagccttaac tggcacgtaa
     1261 cttcaagact aactcctcta aatcagttac cagtggctgc tgccaatggc gataagtcgt
     1321 gcctttccgg gttggactca agacgatagt taccggataa ggcgcagtag tcggactgaa
     1381 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     1441 ggcgtggaat gagataacgc ggccataaca gcggaatgac accggtaaac cgaaaggcag
     1501 gaacaggaga gcgcacgagg gagccatcag ggggaaacgc ctggtatctt tatagtcctg
     1561 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1621 gcctatggaa aaacgccacc ggcgcggcct ttttcgggtt cttgtttttt gctcacatgt
     1681 ttcttttggc gttatcccct gattctgtgg ataacctcat taccgtcttt gagtgagctg
     1741 ataccgctcg ccgcagccga acgaccgagc gtagcgagtt agtgagcgag gaagcggaag
     1801 agcgccttat gtggtatttt ctccttacgc accggtatat cgttctgcat cctgtctggt
     1861 gtaggaacac tgttattccc cggtgccact ttccgattca aagcgtgtct gtatgctgtt
     1921 ctggagttct tctgcgagtt cgtgcagttt ctcacacatg gcggcctgtg catcagcatc
     1981 gagtgagtcc agtttttcga gcagcgtcag gctctggctt tttatgaatt ttgccatgtt
     2041 aagtgcggtt tgctgctgct tgttcatctt tctgttttct ccgttctgtc tgtcatctgc
     2101 gtcgtgtgat tatatcgcgt accacttttc gacagttttg ccgccggtat tctgccgtct
     2161 gactctttga cgggcatttc tgcctgacaa tactgtcact gccaaaaaac tgccgtgcct
     2221 ttgtcggtaa ttcgaacttg ctgacaggac aggatgtgta attgttatac cgcgcataca
     2281 tgcgcgctat tacaattacc ctggtcaggg cttcgccccg acaccccatg tcagatacgg
     2341 agccatgttt tatgacaaaa cgaagtggaa gtaatacgcg caggcgggct atcagtcgcc
     2401 ctgttcgtct gacggcagaa gaagaccagg aaatcagaaa aagggctgct gaatgcggca
     2461 agaccgtttc cggtttttta cgggcggcag ctctcggtaa gaaagttaac tcactgactg
     2521 atgatcgggt actgaaagaa gttatgagac tgggggcgtt acagaaaaaa ctctttatcg
     2581 acggcaagcg tgtcggggac agagagtatg cggaggtgct gatcgctatt acggagtatc
     2641 accgggccct gttatccagg cttatggcag attagcttcc cggagagaaa ctgtcgaaaa
     2701 cagacggtat gaacgccgta aggccccaaa ccgatcgcca ttcactttca tgcatagcta
     2761 tgcagtgagc tgaaaacggt cctgacgcat ttttccggtt ttcactgggt aaaacatctc
     2821 tttttgcggt gtttgcggca gaatcgcgtt cagcgcgttt tagcggtgcg cgtaagggga
     2881 gttatggtaa attggcagca aagtattttc tgatgttgtg gattgtgaag agtattcaat
     2941 tcttattatg aaacaataac tataactggt tgtttcatat agataaatat aacaaaaaag
     3001 cccactctgt agtgggcttt tttgttatat tttacgctct agaggtacac agaaaaacgg
     3061 tagttcgcct gctcctcgaa tgttgtaatg gtagtctgaa aaatattcat agtaaagaga
     3121 taagtgagtt ttc
//
