LOCUS       NZ_CP142050             3374 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Escherichia coli strain L9 plasmid pL9-6, complete sequence.
ACCESSION   NZ_CP142050
VERSION     NZ_CP142050.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39307979
            Assembly: GCF_035666355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Zhang,L., Tao,M., Li,M. and Liu,J.
  TITLE     Effect of colistin on Escherichia coli biofilm
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Zhang,L., Tao,M., Li,M. and Liu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2024) College of Veterinary Medicine, Henan
            Agricultural University, Longzihu District, Zhengzhou, Henan 450046,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-JUN-2023
            Assembly Method :: A5-miseeq v. V20152522; CANU v. v1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina TruSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 07:58:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,001
            CDSs (total)                      :: 4,880
            Genes (coding)                    :: 4,623
            CDSs (with protein)               :: 4,623
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 70 of 257
            Pseudo Genes (incomplete)         :: 192 of 257
            Pseudo Genes (internal stop)      :: 51 of 257
            Pseudo Genes (multiple problems)  :: 51 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142050.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="L9"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pL9-6"
                     /geo_loc_name="China:Henan"
                     /collection_date="2023-06-13"
     gene            complement(872..1105)
                     /locus_tag="VPJ62_RS24950"
                     /old_locus_tag="VPJ62_24950"
     CDS             complement(872..1105)
                     /locus_tag="VPJ62_RS24950"
                     /old_locus_tag="VPJ62_24950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137389.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1112..1672)
                     /locus_tag="VPJ62_RS24955"
                     /old_locus_tag="VPJ62_24955"
     CDS             complement(1112..1672)
                     /locus_tag="VPJ62_RS24955"
                     /old_locus_tag="VPJ62_24955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_149781275.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISGHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1704..2360)
                     /locus_tag="VPJ62_RS24960"
                     /old_locus_tag="VPJ62_24960"
                     /pseudo=""
     CDS             complement(<1704..2360)
                     /locus_tag="VPJ62_RS24960"
                     /old_locus_tag="VPJ62_24960"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2350..2673)
                     /locus_tag="VPJ62_RS24965"
                     /old_locus_tag="VPJ62_24965"
     CDS             complement(2350..2673)
                     /locus_tag="VPJ62_RS24965"
                     /old_locus_tag="VPJ62_24965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001696562.1"
                     /translation="MLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTGKLPTL
                     APPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVREQGVR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2737..2928
                     /locus_tag="VPJ62_RS24970"
                     /old_locus_tag="VPJ62_24970"
     CDS             2737..2928
                     /locus_tag="VPJ62_RS24970"
                     /old_locus_tag="VPJ62_24970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165979.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(3010..3090)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="93.0"
                     /evalue="4.04e-16"
     Typing          complement(790..2360)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.006"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggtggaag gtcggaggga acggcaacaa ctcacccctc ccagtgaagg gacgggtagt
       61 ttgccgctct cgatatgccg gacggagcca aggccatcga tcacggggcg cgtcagtgga
      121 agaacccgcg cctgcgtgac gtatgcgtct tcgccgcacc atctcacccg tcacgccacc
      181 acataccggg aactgccgcc aaggtctaac tcaaccaatc tagccgcgct acatacggtt
      241 tttgtggcaa ctcatcaggt gtccgctgtc gatgaaactc tctgcgccgg tagcggaact
      301 ctcacgcgtg cggtcgccgt cgtcacagct cccccaaccg caaacgattc tcgcaaatac
      361 gcaagtatct gcaaaaacag gtttttttcg gggttcctgg gagactgtgc tgttgaaaaa
      421 ttcttgaaga atacagaagg ctcatgcaaa ctgaatgaac tggtttctgt tgtggatctc
      481 aacagtttcc acaggaacat tgaggaggtc agagacctca atgaacccga aacagctcgc
      541 cttcctggcg agctgttttc gttctacgcc cgccgacgaa agatcgcaag tgcaataacg
      601 agatttttca cgtctttacg ctcaaaaagt aaaaaaacca tgttttcccc tggctgaatc
      661 cccaaaaacg cgtgaaatgc gttttcagga tgttgcatag ctatgcacaa atacggcaca
      721 cgatttgttt cgctcgcccc agccgtcgaa cgggtcaaaa tggcaagatt tcgtcacttc
      781 cccggtcaca tctccattga tggcccactc cgaacccgtg ccgcgcgttc ctgtacctca
      841 tgtctgtacc gggcacgctc ctgttcatcc cttagctgtt gttcgcgata aataaccgac
      901 tcaagcgatc cacttgcccg gctaatctct gcacctgctg actcaatatc acgacgcgct
      961 cgttcagtat cgcgttgttc cgttgcgtaa cctcgtgcat cttctgcaat tccgcgaagg
     1021 cgctctccca ctcgttcagc cgccgcatat agtcctgttg caaatgctct aaggcgttca
     1081 gcaaatgccc ttccagttcc gtcatactcg tttactccct gaccagcctc acggcgttct
     1141 tgtcgcccac cgtccaggtg ttttcccctt tcaccccgtc cggcaacacc agaaatttcc
     1201 cgttctcgcc attgacgaac gtcacccccc aggtttttgc ctcgagtttc gccagcgtgt
     1261 tttcctgctc tctgatagtc aggatatttt tcgcaatctt gctttcctgc cactccatca
     1321 gcccccagca ccccaggaaa atcccgcccg tgacgctgag cgcggtcagc gccggaaaca
     1381 gtatccgccc tttgatcacc cgccagagca gttttttctg ctgccgggac agttcggcct
     1441 ctgtggtgct gaactgcatg ctcacggtgg tgctcagcgt atccagttgc tttttcacgg
     1501 atgttgtatg tccgctgata gcgtctctga ttttctgcct gtttaagctc agtgccttgt
     1561 ctacagactc ttcgagcttc ctgaactcgc tgttcagcat gttctcggta gaagcggctc
     1621 gctctttcag tttcttctcg aagtctgttc ccatttgtaa aagattgctc atacagcgcc
     1681 ccctttagcc tgagattccg cccaccctcc gggtcggcaa tactgatact gcttctggtt
     1741 gtcctcacca cctcaaaacc tgccgctgtg agcgcctcag tgacatcctg acgcgttttt
     1801 attgctccgg catggtaaag cgcctcaaga ccccgtgtaa tcgcttctgc ggcctcctgt
     1861 ttcgcttttg gcagattatt cggtgtgaca agcgtccgtc tgttctccgg tgcgttcggg
     1921 tcacgcagcc cgtaatgatg attaaccagc gtctgccagg catttatcct cggcctgtcc
     1981 gcacggtcgt agtacggctg gaggcgctta ccgctctgta gctccatgtt cgggatgaca
     2041 aaattcagtt cgagccgccc cttatcctgg tgttccaccc aaagaatgct gtactggtct
     2101 ttttcgagac cgggcataag aacacgctca aagctcgtca tcactctttc gcgttcaccc
     2161 ggcggtaact ccttctccgc gaacgacaga acgcctgacg tgtatttctt cgaaaatggc
     2221 gtggaatcaa tgagttcccg cacttcttcc gggttacccc ggagcaccgt tgcgccttcg
     2281 cggttacgct cccgccccag caggtaatcg accgggccac ttccaccgcc tttccccctg
     2341 gcatgaaatt taactatcat cccgtacccc ctgttccctg acagcctgcc gcaactggcg
     2401 caattcaccc tcgatggcca tcagtgcggc caccacatga acccggtcaa tcgaagacca
     2461 ctggccgctg ttcaccttcc gggctgtctg gttcaggttg ttcccgatgg cagccagatg
     2521 ccgcagcaat ggcggtgcca gtgtcggcag ttttcctgtg cgtgcaaccg gctcccccag
     2581 acagacccgc cgcatccatt ccgccagtcg tttaccctcg cagcgttcca gtaaccgggc
     2641 gtgctcctca tcggtaaccc gtatcgtgag catcttctcg cgtttcatcg gtatcattcc
     2701 ccctaacaaa tcccccatac aaggagcctt aagaccatga ccaaacagga aaaaacagcc
     2761 cttaacatgg ctcgctttat cagaagccag acgctaaccc tgctggaaaa actcaacgat
     2821 ctggacgcgg acgaacaggc agacatctgc gaatcacttc acgaccacgc cgacgaactc
     2881 taccgcagtt gcctcgcacg cttcggcgat gacggtgaaa gcaactgaaa catgcagctc
     2941 ccggagacgt tcacagcttg cctgtgagcg gatgccggga gcagacaagc ccgccagggc
     3001 gcgtcagtgc gggtgtcggg gcgcagccct gacccagttc acagagcgat agcgatgtgt
     3061 atactggctt tcctcatttt gcatcggtgc agagtggatg ggaagtgcac catgtcagga
     3121 gtaaaaagcc gcacagatgc gtaaaaacag cgcgcccgat aaacaacaca cactcggaca
     3181 ctggcacagg cgcgtaagtg cacaatgctt gcctctgtga ctcttccgct tcctcgccca
     3241 ctcgctcact acgttcggtc gctggggctg cggcgagcgg tgggatcact ctcgttgtga
     3301 tgcagatccg tttttttttt ttgcactgga gttatcagga gtagcggtgc acatgggatc
     3361 gtgcgatatg ctgg
//
