LOCUS       NZ_CP142049             3406 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Escherichia coli strain L9 plasmid pL9-5, complete sequence.
ACCESSION   NZ_CP142049
VERSION     NZ_CP142049.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39307979
            Assembly: GCF_035666355.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Zhang,L., Tao,M., Li,M. and Liu,J.
  TITLE     Effect of colistin on Escherichia coli biofilm
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3406)
  AUTHORS   Zhang,L., Tao,M., Li,M. and Liu,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-2024) College of Veterinary Medicine, Henan
            Agricultural University, Longzihu District, Zhengzhou, Henan 450046,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-JUN-2023
            Assembly Method :: A5-miseeq v. V20152522; CANU v. v1.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 432.0x
            Sequencing Technology             :: Illumina TruSeq; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/17/2024 07:58:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,001
            CDSs (total)                      :: 4,880
            Genes (coding)                    :: 4,623
            CDSs (with protein)               :: 4,623
            Genes (RNA)                       :: 121
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 257
            CDSs (without protein)            :: 257
            Pseudo Genes (ambiguous residues) :: 0 of 257
            Pseudo Genes (frameshifted)       :: 70 of 257
            Pseudo Genes (incomplete)         :: 192 of 257
            Pseudo Genes (internal stop)      :: 51 of 257
            Pseudo Genes (multiple problems)  :: 51 of 257
            Pseudo Genes (short protein)      :: 4 of 257
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP142049.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="L9"
                     /isolation_source="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pL9-5"
                     /geo_loc_name="China:Henan"
                     /collection_date="2023-06-13"
     gene            1..273
                     /locus_tag="VPJ62_RS24930"
                     /old_locus_tag="VPJ62_24930"
     CDS             1..273
                     /locus_tag="VPJ62_RS24930"
                     /old_locus_tag="VPJ62_24930"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_324752158.1"
                     /translation="MKILAAMEIADCDFEHMDMDVLVGALLKLKLWDSHHHNLNMQGKR
                     SFLDDLRADGIEAIKKYEAEIESENESTYRKLKNQSIPLSTPYAT"
                     /name="hypothetical protein"
     gene            complement(388..1587)
                     /locus_tag="VPJ62_RS24935"
                     /old_locus_tag="VPJ62_24935"
     CDS             complement(388..1587)
                     /locus_tag="VPJ62_RS24935"
                     /old_locus_tag="VPJ62_24935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="WP_072648941.1"
                     /translation="MLSGFTPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKMLACGT
                     RILGVKEFGCDNPDCQHVKYLTNSCGSRACPSCGKKATDLWTATQLNRLPDCDWVHLVF
                     TLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLNWHPHVH
                     VSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGMAMPESLSHITTESQW
                     RSLVLKSGGKYWHVYMSKKTAGGRNTARYQGRYLKKPPIAASRLAHYNGGASLSFRYLD
                     HKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKP
                     EPVAKVCYAQMVKQFLSRDPFECVLCGCRMVYRRAIAGLNVSGLKKNARDISLLRYMPA
                     "
                     /name="IS91 family transposase"
     gene            complement(1597..1785)
                     /locus_tag="VPJ62_RS24940"
                     /old_locus_tag="VPJ62_24940"
     CDS             complement(1597..1785)
                     /locus_tag="VPJ62_RS24940"
                     /old_locus_tag="VPJ62_24940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000957857.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVTTSIL
                     KRRGDTAALTLLQDTGV"
                     /name="hypothetical protein"
     gene            2957..3193
                     /locus_tag="VPJ62_RS24945"
                     /old_locus_tag="VPJ62_24945"
     CDS             2957..3193
                     /locus_tag="VPJ62_RS24945"
                     /old_locus_tag="VPJ62_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016230891.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          2716..2800
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.512"
                     /coverage="100.0"
                     /evalue="3.06e-32"
ORIGIN
        1 atgaagattt tggctgcaat ggaaatagct gattgtgatt ttgaacatat ggatatggat
       61 gtcttagtag gggctttact gaaattaaaa ttgtgggata gccaccatca caacttgaac
      121 atgcaaggta agaggagctt tctcgatgat ttaagagcgg atggtattga ggctattaag
      181 aaatacgagg ctgaaattga atctgagaat gagtccacgt ataggaaatt gaaaaaccag
      241 tcgataccat tgtccacgcc ttatgccacg tgagcctttc tgcttttccc accactccgg
      301 tggtttccct ctctgtatgg tcgattttta taccgtaaca ggctgttttt tatactttca
      361 ccgattcccg ggcacactta gcctgtgtca ggccggcata tacctcagta gactgatatc
      421 ccgcgcgttt ttcttcagcc ctgatacatt cagccccgca atggcccggc ggtataccat
      481 ccggcagcca cacagcacgc attcgaacgg gtcacgactc aggaactgtt tcaccatttg
      541 tgcatagcac actttcgcca ctggttccgg tttatccatc cccagtgcac ggtacacctg
      601 cggcagcttc tctccacaca cacggttggc aaggaacccg aagtacctca ccatcttaaa
      661 aaacttctcc gggatgtgct gtttcagcct cgcgaccagc tcacgctgtg tcagcgtttc
      721 cgtcgccgtt tctcccgttt tgtggtccag gtaacggaag ctcaggcttg cccctccgtt
      781 gtaatgagcc agtcgggaag ccgctattgg tggcttcttc agataacgac cctggtagcg
      841 tgccgtattc cgccctccgg ctgtcttctt cgacatgtac acatgccagt attttccgcc
      901 ggatttcagc accaggcttc tccactgtga ttccgtcgtg atatgtgaca acgactccgg
      961 cattgccatc ccctctgacc acgctttcag aagcagctgc cgcatattcc acatccaccg
     1021 tgaacgcatc gcgtctttca ggaagctcag ctttttccac tgaccatgct tattcagacc
     1081 tccacaggtt acagacacat gtacatgcgg atgccagttg agacgacggc catacgtgtg
     1141 gatggcgcag aagataccgg gttccagccc ccgttttcgg gcggcataca gcagattctc
     1201 caccgccaga cggcacacgt cattcagcag ccagcggttg ctttcgaaca ccggccacag
     1261 cgtgtccggc agggtgaaga ccagatgtac ccagtcgcag tcaggaagac gattcagctg
     1321 tgttgctgtc cacaggtctg tggccttctt tccgcaggac gggcaggcac ggctgccgca
     1381 tgagttggtc aggtacttta cgtgctgaca gtccgggtta tcacacccga actcttttac
     1441 acccagtatc cgtgtgccgc aggccagcat tttggtgacg gcttcaacct cgatatcgcg
     1501 cagaccgccc gcatccagga aggacgtcca gcactggttg gctgtgaaca gacgtttcag
     1561 agggcgggga gtaaaaccgg acaacatgag gatggattaa accccggtat cctgtagcag
     1621 ggtaagggct gctgtatcgc cacggcgctt caggatggag gtcgtcacca tcgcaggtgg
     1681 tgggtccatg ggtttgagca ttttcatcca gtcccattct cgttgtgaga gcttctcagc
     1741 accaacagag agcagaactg tatcgatact tttgcgggtc aacataaggg gatgagtcct
     1801 taataaaaca ggtcctcaga gcatacatat tctgcggggc gtgcggcaac aacaggacga
     1861 accggggcgg ttttcaattt gcagccgcca ggctgccgtg gttctttgcg gaggagcgat
     1921 taatactgag gatctatcag aacgagcttg gcttatggtt agggaggctc ttttgtggtg
     1981 aggctgagat cggtcagtgt ggtgtttatg gatcggctga tgtggttcga acagctgcgc
     2041 gtcaaagcgg tattttgtgc gatagcatcg aaatttttca aaaggatgcc taatgccccc
     2101 gtggtgggta caccagaaaa cggggagcgg agcgaccagc gtcaggtcgt tctatgttgt
     2161 tctgattgtg tgggtgttgt gccagactgg gcaatcgcat ttcaccgcaa ctcctgcctt
     2221 gaatctcact ttatcaggtg cctgttatcg tacagcgaga cggggtagag cttaactctt
     2281 tcgcggttaa gttacgtttc caccgtttgc ctgtttatcc tgccggagtc cggggcgtag
     2341 acctgagttt caagggtgca gatatgattt taatgtcgtt gaatggtatt aagctggtag
     2401 tgacaaggct agccagccct gctaggatag gattgctatg tctgatcctg gcaggtatct
     2461 gcgaagaatt agagtaccga agccctgtga ttctgtcacg gggcttttgt acatctgtag
     2521 cctgatcttt caacaagatc cccgtcacac ctcacttcat gtatgtgaaa aaagttgtag
     2581 tgcccgccgc agtctcacgt ccggagcgta gcgaccgagt gagcgaggag gcatcatttc
     2641 actattcggg catggtattg cggacgcttc tgagccacat atcacacctg ccacatgacg
     2701 cactttcctg ataaccgcac ctatgccaac atattaagcc agtatacact ccgctagcgc
     2761 tacgtgactg gttcagggct gcgccccgaa acccgctaaa tccactgacg cgcctgcggc
     2821 ttgtccaacg cctcacggca ccggattaaa atccgctccc gttcaccgtt tctgactgcc
     2881 tttcaccgtc gcttacgctt catcgttcct actgtgtctg gcagactgca ggggctattc
     2941 tcatacggag ataaacatga cgagactgac aaacctgacc cccgctgaga aaaagtttat
     3001 cgacgacgct atagccgctg ccgaacgtgc ggccgggaag aagctcaatc agcctaaccg
     3061 gcacatcgtc ctgaacagag cgcgtgcgca gattgagtcg cagcgttacg ccgaccgcca
     3121 gcgtgcatta cgtgaggatg agcggcagca gtccgatttt gcctggtcac gtccgcgcgc
     3181 gccgcgccgc tgattgcgga ttttggcggt ttagtggtgc tgatgctgcg aaatcacctg
     3241 cggttcaccc gcgtgcatag ctatgcagtc gcgcagaaat catcaggggc gtttttccgg
     3301 gatgtgggtg aggaaatgtg ccggaaacgg ctctgatgcg tcagaattgc gctgagggcc
     3361 tgtcaggggg tgctgataac ggatgttatg gtaaatcgga gggtgt
//
