LOCUS       NZ_CP141991             5098 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KC25-20651 plasmid pKC25-20651D,
            complete sequence.
ACCESSION   NZ_CP141991
VERSION     NZ_CP141991.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39278676
            Assembly: GCF_035658455.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5098)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Whole-genome sequencing of the Klebsiella pneumoniae strain
            KC25-20651
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5098)
  AUTHORS   Chen,M. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-JAN-2024) Zhuhai hospital, Jinan University, 79
            Kangning Road, Zhuhai, Guangdong 519000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. 0.13-r308; Canu v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 219.28x
            Sequencing Technology             :: Illumina; PacBio
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 23:06:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,557
            CDSs (total)                      :: 5,431
            Genes (coding)                    :: 5,259
            CDSs (with protein)               :: 5,259
            Genes (RNA)                       :: 126
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 75 of 172
            Pseudo Genes (incomplete)         :: 92 of 172
            Pseudo Genes (internal stop)      :: 32 of 172
            Pseudo Genes (multiple problems)  :: 25 of 172
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141991.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5098
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KC25-20651"
                     /isolation_source="mid-stream urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKC25-20651D"
                     /geo_loc_name="China: Guangzhou"
                     /lat_lon="23.09 N 113.28 E"
                     /collection_date="2023"
                     /collected_by="Mingxiao Chen"
     gene            1..249
                     /locus_tag="VN981_RS27740"
                     /old_locus_tag="VN981_27740"
     CDS             1..249
                     /locus_tag="VN981_RS27740"
                     /old_locus_tag="VN981_27740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064183951.1"
                     /translation="MAWYIDSKNVKENKVETANEKYKKIWKPGEKPSFAGIYRCQSCGY
                     EDLINRQCTKLPPCSNCEEKGHKNNTWKLLVRAVDAE"
                     /name="hypothetical protein"
     gene            complement(996..1397)
                     /locus_tag="VN981_RS27745"
                     /old_locus_tag="VN981_27745"
     CDS             complement(996..1397)
                     /locus_tag="VN981_RS27745"
                     /old_locus_tag="VN981_27745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1397..1588)
                     /locus_tag="VN981_RS27750"
                     /old_locus_tag="VN981_27750"
     CDS             complement(1397..1588)
                     /locus_tag="VN981_RS27750"
                     /old_locus_tag="VN981_27750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2004..2154
                     /locus_tag="VN981_RS27755"
                     /old_locus_tag="VN981_27755"
                     /pseudo=""
     CDS             2004..2154
                     /locus_tag="VN981_RS27755"
                     /old_locus_tag="VN981_27755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396189.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2401..3270)
                     /locus_tag="VN981_RS27760"
                     /old_locus_tag="VN981_27760"
     CDS             complement(2401..3270)
                     /locus_tag="VN981_RS27760"
                     /old_locus_tag="VN981_27760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015387122.1"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="WP_064183947.1"
                     /translation="MKSIRNQSFIEERLWYIEWLLMMRGWLSRIDLMDKFGIKGAAASR
                     DIKRYKEQSDENLYLDHRVKRYVADLDKFKPVYETSEELIFSKLTDLDESKSLGAGSPL
                     IETIPRLAEINPKDITGITRAILNMNVLKIAYCSMTSGTSEKKIVPHSMFHNDLKTYVR
                     CYDLGRNRFLDLVVGRILEILGEDGAAPAECLREFDDDWNTYISLELVVHPRIQHKKAI
                     ESDMQMTDGVKVIEVRKSLAQYWLRRWCVDCSATAKMTDKAFQLHLRNSSVINGVNGAF
                     PGVNNHID"
                     /name="WYL domain-containing protein"
     gene            3374..3550
                     /locus_tag="VN981_RS27765"
                     /old_locus_tag="VN981_27765"
     CDS             3374..3550
                     /locus_tag="VN981_RS27765"
                     /old_locus_tag="VN981_27765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162919229.1"
                     /translation="MKMATNKPYGDGHRIGAVKQRSQFQTPTGLWAKRDSKTGRIMDVK
                     QDGTPFKGVRKEK"
                     /name="hypothetical protein"
     gene            3898..4128
                     /locus_tag="VN981_RS27770"
                     /old_locus_tag="VN981_27770"
     CDS             3898..4128
                     /locus_tag="VN981_RS27770"
                     /old_locus_tag="VN981_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003539161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074377679.1"
                     /translation="MALYKNGNALQHSNHQAFDAELGPGTQAPNPGIYRCVNCGDEIGI
                     AKGHTLPPQNHHQHSNGLPIKWKLLVCAIQK"
                     /name="hypothetical protein"
     gene            complement(4219..4428)
                     /locus_tag="VN981_RS27775"
                     /old_locus_tag="VN981_27775"
     CDS             complement(4219..4428)
                     /locus_tag="VN981_RS27775"
                     /old_locus_tag="VN981_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004196825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074377680.1"
                     /translation="MEWVIGGFVVLIVLGAIFKPSRCDVCGTGFKKKYYTWTIEGKKQH
                     LCPNCNSKMSRRVSNQRFTDRFGR"
                     /name="hypothetical protein"
     gene            complement(4466..4732)
                     /locus_tag="VN981_RS27780"
                     /old_locus_tag="VN981_27780"
     CDS             complement(4466..4732)
                     /locus_tag="VN981_RS27780"
                     /old_locus_tag="VN981_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_064183949.1"
                     /translation="MVWTINYSERALKSLRKMDKQTARRILDFMDLRIAVAEDPRQSGK
                     PLKGELGEFWRYRVGDYRILCEILDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4716..4940)
                     /locus_tag="VN981_RS27785"
                     /old_locus_tag="VN981_27785"
     CDS             complement(4716..4940)
                     /locus_tag="VN981_RS27785"
                     /old_locus_tag="VN981_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraY domain-containing protein"
                     /protein_id="WP_064183950.1"
                     /translation="MLAIRLSAEIESRLDLLAKKTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEAVRKSLGLDD"
                     /name="TraY domain-containing protein"
     Typing          1823..2330
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.205"
                     /coverage="99.0"
                     /evalue="2.75e-180"
ORIGIN
        1 atggcatggt atatcgatag caaaaacgtc aaagaaaaca aagttgaaac cgcaaatgaa
       61 aaatataaaa aaatctggaa accaggggaa aaaccatctt ttgcaggaat ctatcgctgt
      121 cagtcatgtg gatatgaaga tttaatcaac cgccagtgca ccaagctccc accatgcagc
      181 aattgcgagg aaaaagggca taaaaacaac acatggaaac tgctggtaag agcggttgat
      241 gcggaatagt caaaccggat tgaacataac accttaattg tacgcaccgc taaaacggcc
      301 tcaggacgtt tctgacggga aaccggtaaa acaggcaaca aaccacccga aaacccgcca
      361 gaaacgcgca ggctctgttt ttacggcatg catgagtatg cgcgcaagtg catagcgcgg
      421 aaattcacgc cattcagcca gggtaagtcc tgtttccggc gttcttctga tccgaaaacg
      481 gtgaacgggg acgcaattta ttgcgtggcc gtgaggcgtt gccggcgcag ccggaaaacc
      541 ggttttcagc acgttcagga atcgggttcg tcgacggccg gcaatcacgt tatggcaagt
      601 cgccggacga ctcaccacac gattcaccgg cgattcgaat cgtgaattga atcaagagtc
      661 accatgaaca aaaaacgccc aaattgatga attgaatcaa gagtcgattc atcataacaa
      721 catgataata aagcgtgatt cacatatgaa tcgtgggcaa ttcgtaaacg attcgccata
      781 gagtcgtcca cgacttggat ctgcactttg taacacatca ctgcacatta atttttcatt
      841 aaccaattgt tttaaataag gaatctgcaa aattttaatt ttgcgtagtg tgtgggtatt
      901 tttagtcgtg aggagcgttc agagatctgg cgatttttgg gaaggttcag gaactccctg
      961 ggatggccgg agaggaaaac tcaagcatgg caggatcagg attttttcca gaatttccac
     1021 catggcgccg gagattcagt taattttgcc tggtgatttt caagaaggag catggcctgt
     1081 ttcaggctgt caatgtgcga atccttggac aaaagtaagg tgttcaggtg ttcaatctcc
     1141 tgtttcagat gacgaatcac ctgttcactc tggtcagcag aggctgtatc acgctgttca
     1201 gagacctgtc cagaatggtg ttcacccttc ggttgttcaa agggaccaaa gacacgtaaa
     1261 agctctgaag tatctaccat ttttgcattg cgttcaccgc tacaggtgga caggtcacct
     1321 gctttaatta acctgtgcag tgtcgttcta ctcttacccg tcagtcgggc agcctcacta
     1381 atagacactc tggccatcag ttaccatcct gtaatgcatc cagtctcgag gacagtgtac
     1441 ggaaaagatg ctccgcatca tcgtggagtt tctcacaaag gtcggcttcg acatcgagat
     1501 caagctcgtt aagcttctcc agcaggagca gagactgatt ctggatgaat ttagccatgt
     1561 tcagcgctgt tttctgttgt ttagtcatag aggcctcaga gcgtcagtac tcagtgtagg
     1621 gatgttctct tttccgcttc ctcgctcact cagtcgctat gctcctgccg ttcggctgcg
     1681 gcgagcggca ggagcatgcg tataaaagct cgagaaaacc gagcggtcgc gtaacgggga
     1741 aaaggaaagg ccgcaaggcg gccctttttt ccataggctc cgcccccctg acaagcatca
     1801 cagaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccagga
     1861 gtttccccct gatggctccc tcgtgcgctc tcctgttcct gcctttcggt ttgccggtgt
     1921 cattccgctg ttatggccgc gtttatctca ttccacgcct gacactcagt tccgggtagg
     1981 cagttcgctc caagctggac tgtatgcacg aaccccccgt tcagtccgac tgctgcgcct
     2041 tgatccggta actatcaact tgagtccaac ccggaaagac acgacaaagc gccactggca
     2101 gcagccactg gtaaacagga tgtgcgagag atgtatttag agttcttgaa gtgaagacct
     2161 aactgaggct acactggaag gacggatttg gtgacacgtc tcgcacaaga ctgttaccac
     2221 ggttcagaaa cttgccggtt aatgaacctt cgaaaaacca ccttgccggg tggttttttc
     2281 gtttacagag caagagatta cgcgcagaaa aaaaggatct caagaagatc cttttcagaa
     2341 cgacgaatac catcatttca tcgacagcag ttcttcatca tccacgagca aacaacacaa
     2401 tcaatcgata tggttgttta ccccagggaa agcaccattg actccattaa taacgctgga
     2461 atttctcagg tgaagctgaa aagctttatc ggtcatcttt gccgtcgcag aacaatctac
     2521 acaccatcga cgtagccagt actgagccag tgactttctg acttcaatca ctttcacacc
     2581 gtcagtcatt tgcatgtcac tctcaatagc tttcttatgc tgaatcctag gatgaacgac
     2641 caactccaaa gaaatatatg tgttccaatc atcgtcaaac tctctcaaac attccgcagg
     2701 agctgcccca tcctcaccca gaatctcaag aattcgccca acaacgagat ctaaaaaccg
     2761 atttcgcccg agatcatagc aacgaacgta tgttttaaga tcattatgaa acattgaatg
     2821 gggaacgatt ttcttctctg atgttcctga agtcattgag caataagcta ttttaagaac
     2881 attcatattt agaatcgccc gagtaatccc cgttatatcc ttcgggttta tttcagccaa
     2941 gcgagggata gtttcaatca gtggggaccc cgcacctaaa gacttcgact catctagatc
     3001 cgtaagcttt gaaaagatga gttcctcgga ggtttcgtaa actggcttaa atttatcaag
     3061 atcagcaaca tatctcttaa ccctatgatc taaatataaa ttctcatcac tctgttcttt
     3121 atatctttta atgtcacgac tggccgcagc tcccttaatc ccaaatttat ccattaagtc
     3181 tatgcgggat aaccatcctc gcatcatcaa aagccattct atataccata gtctctcttc
     3241 tataaaactc tgatttctaa tgcttttcat aagataacaa tcctagaaat tattgtaccg
     3301 atcaaaagtt gaactaatct tgaagatcac ttatcattaa gataacagta gcatacttca
     3361 cgataggaga aggatgaaaa tggcaacaaa caaaccatat ggcgatggcc atcgcattgg
     3421 cgcagtaaag caacgttctc aattccagac ccccacaggg ttatgggcaa agcgagattc
     3481 aaaaacaggg aggattatgg atgtgaaaca ggatggaacc ccattcaaag gggtaaggaa
     3541 ggaaaagtga ttgggttagc ctgtaccacc agaactaacc ctaacaaacc gtacgcaaaa
     3601 gcggcgcttg agtaccacct caagtgcttt tataagccag ccactagatt tgttgagtgc
     3661 tacagtacaa ttctgagcgc aaaaatcaac attcaagtgg cgaaatttgg gaaatgcgac
     3721 atgcagacag acgagtctca ggcatacatt tttcgccctt acataacggt aaagggtaaa
     3781 cggatcaccc gtcctaacgg gggaatgttc aagatcccga ttaatcggga aaagaaaaaa
     3841 tagcaatctt cgcagccgtc aggctgcgac ccaatacgtt tatgcaagga aaacagaatg
     3901 gcactctata aaaacggcaa tgctttacaa cacagcaatc atcaggcttt tgatgctgaa
     3961 ttagggcctg gtacccaagc ccctaatcct ggaatctacc ggtgcgttaa ctgtggagat
     4021 gaaatcggga tagctaaagg gcatacctta cctccacaaa accatcacca acattcaaac
     4081 gggcttccaa tcaaatggaa attgctcgtc tgtgctatcc agaagtgatg tttgaacaaa
     4141 aagccctgcg gggctttttt tacgccttct gccggtaact atttaccata accccctaat
     4201 tatcagcatc gagacagact atcgtccaaa cctgtcagta aatcgttggt tactgaccct
     4261 tcggctcatt ttgctattgc aattagggca caaatgctgc tttttgcctt caatagtcca
     4321 ggtgtaatat tttttcttaa aacctgtacc acaaacatca cagcgacttg gtttaaaaat
     4381 cgcaccaaga acaataagta caacaaaacc accaataacc cattccataa aatccctctc
     4441 aatgaaaaag cccgctatgc gggcttcagt cgtaaacttc gcgtcggtgg ccgattgtag
     4501 ccgcaaggat gaccagctca tcatctagga tttcacacag aatacgataa tcgcctaccc
     4561 gataacgcca gaattcacct aactcacctt tgagcggttt ccctgactga cggggatctt
     4621 ctgcaacagc aatgcgtaag tccataaaat ccaaaattcg tcgtgcagtc tgcttgtcca
     4681 tcttacgtaa cgattttaac gcccgttcgg aatagttaat cgtccagacc aagtgacttc
     4741 ctcacggctt cagacgaatg cacagcctca tcaccacgcc gaacgcgtgc ggcagtttct
     4801 gctgaaagat agtaatcttc cagatcttcc aagtgtgcaa gtatcgcttc ccgcgcatag
     4861 aacgtctttg ttctgccggt ttttttcgcc agtaaatcaa gacgagactc aatttcagca
     4921 gacagccgga tagccaacat gcagacctcc tgatctcaaa atgctataca tgtatatcaa
     4981 gtataaaaca gttagatcga aaggcaaaaa aataatctgc acagagttga gaaatgcacc
     5041 ataagaccac aataattaag tgagtaaatg tccttcaaac ttatacggaa acgacata
//
