LOCUS       NZ_CP141851             3223 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP111_WM3_19.23_ST846_KPC2 plasmid
            pKP111_unnamed2, complete sequence.
ACCESSION   NZ_CP141851
VERSION     NZ_CP141851.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN39181636
            Assembly: GCF_035658195.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: JUN-2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Assembly Name                     :: q20run15_BC09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 46x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 23:07:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,429
            CDSs (total)                      :: 5,307
            Genes (coding)                    :: 5,155
            CDSs (with protein)               :: 5,155
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 152
            CDSs (without protein)            :: 152
            Pseudo Genes (ambiguous residues) :: 0 of 152
            Pseudo Genes (frameshifted)       :: 73 of 152
            Pseudo Genes (incomplete)         :: 78 of 152
            Pseudo Genes (internal stop)      :: 23 of 152
            Pseudo Genes (multiple problems)  :: 20 of 152
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141851.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP111_WM3_19.23_ST846_KPC2"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP111_unnamed2"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2023-05-02"
                     /genotype="ST846"
     gene            complement(20..352)
                     /locus_tag="VI618_RS01115"
                     /old_locus_tag="VI618_01115"
     CDS             complement(20..352)
                     /locus_tag="VI618_RS01115"
                     /old_locus_tag="VI618_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrE family multidrug efflux SMR transporter"
                     /protein_id="WP_001099531.1"
                     /translation="MNTYIFLGGAIVAEVIGTTFMKYSEGFTRLWPSVGTAISYCAAFY
                     LLSQTLENIPTGIAYAIWSGAGIVLISLAGWLVSGQKLDWPAIAGMFLICVGVLVINIF
                     SKSSAH"
                     /name="EmrE family multidrug efflux SMR transporter"
     gene            complement(520..735)
                     /locus_tag="VI618_RS01120"
                     /old_locus_tag="VI618_01120"
     CDS             complement(520..735)
                     /locus_tag="VI618_RS01120"
                     /old_locus_tag="VI618_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_024195375.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLIQRENA
                     ALNERVTVLSRQVQSLSEQLRRLSKG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(739..1224)
                     /locus_tag="VI618_RS01125"
                     /old_locus_tag="VI618_01125"
     CDS             complement(739..1224)
                     /locus_tag="VI618_RS01125"
                     /old_locus_tag="VI618_01125"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_025367738.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     ENYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVRVNPEAGQFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1259..1963)
                     /locus_tag="VI618_RS01130"
                     /old_locus_tag="VI618_01130"
                     /pseudo=""
     CDS             complement(<1259..1963)
                     /locus_tag="VI618_RS01130"
                     /old_locus_tag="VI618_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1902..2225)
                     /locus_tag="VI618_RS01135"
                     /old_locus_tag="VI618_01135"
     CDS             complement(1902..2225)
                     /locus_tag="VI618_RS01135"
                     /old_locus_tag="VI618_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_004193914.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQTCLDEKPARAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGSGHDRVQIVAALMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             complement(21..352)
                     /name="qacG"
                     /gene="qacG"
                     /gene_name="small multidrug resistance (SMR) antibiotic
                     efflux pump"
                     /tool="rgi"
                     /drug_class="disinfecting agents and antiseptics"
                     /identity="41.35"
                     /coverage="102.8"
     Typing          complement(2322..2513)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="92.308"
                     /coverage="89.0"
                     /evalue="3.38e-71"
     Typing          2493..2976
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.756"
                     /coverage="93.0"
                     /evalue="1.73e-132"
     Typing          complement(423..1912)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.784"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aatatgcttc ggtaaaaagt taatgcgcag aagattttga aaaaatatta ataaccaaaa
       61 ctccaacgca aataaggaac attcctgcaa ttgcgggcca atcaagtttt tgaccgctaa
      121 ccaaccaacc agctaggctt attagtacaa ttcccgcacc agaccaaatt gcgtaagcaa
      181 taccggtagg tatattttcc aatgtttggg aaagcagata aaaggcagcg caataactta
      241 ttgcagttcc tacagacggc cacaggcgtg taaatccttc tgaatatttc ataaaggtgg
      301 tgcctatgac ttcagccaca atagcccctc ccaggaaaat atatgtattc ataaaaatac
      361 ctcatgttga aaaatttaaa tatctttaca taacaactaa ttaatagaac ccggcttatc
      421 atagcgaagg accatgatgc tgccactgcg atcgttccag ctccagcgct ttctggtgct
      481 gcaccgcccg ctcctgctgc cggtgagttt ctatcgcgtt cagcctttcg acaaccggcg
      541 cagctgctcg ctcaaactct gcacctgcct gctcaagacc gtgacgcgct cgttcagcgc
      601 cgcgttctcc cgttgaataa gaccagacat cgtccgccat tccgcgaagg cgttctccca
      661 ctcgtccagc cttttcgagt agtcctgctg tagctgctct aatgcgctca gcaactgttt
      721 ttccagctcc gtcatgtgct atttccccgc cagtatcatc cagctcgagt cctctccgaa
      781 ctgtcccgct tccgggttca ccctcacgca gcggcgtctc tgctcgccgc aggtcgagag
      841 ctgtacgccg ctgttcctct ctgacagcat ggcctgcgtg ctcttctgct cccggatggt
      901 cgtatagttc tcgagtatct gctgcccctg ccaccacaga atgcccgcgc tcgacgctat
      961 cagcagcgcg gagaccagaa cgatggtcag ccacgtctgg ctgaccatgc gcagcatccc
     1021 tttcgtgcgc tggctcatgg ctgaggacag cttccggtcg tggtcgagga tggcggcgct
     1081 gattctcttc tcgctttcgc tcagttccgc tctgacagac ttctcgtgct cgctgaatgc
     1141 ggctttcagc atctcgccgg tagtctgctg ctgcgctttc gatttctgct ctaagtcctt
     1201 cgccagcgtt aaaagactgt tcatagatgg ctcccttcag tcggatgttt cgccccccgt
     1261 ccgggtcggc aatgctgatg ctgctttttg tggtgcgcac cacctcaaaa cctgcgcttt
     1321 ccagcgcctc agtgacgtcc tgacgcgttt tcagctcccc ggatgaggca agggcaagca
     1381 ggccgctcgt aatcgcctgg gcggcttcct gcttcgcttc cggcagcgcg gaaggcgaga
     1441 ccagcacccg ccggttctcc ggcgcgttcg ggtcatgcag ccccagcctg ccgttcacta
     1501 tggtctgcca ggcatcgatg cgcggacggt cggcgcggtc gtaatacggc tgtagccgct
     1561 tgccggtcag aagctccgtg ttcgggatca ggaagttcag ctccagccgc cccttgtccc
     1621 ggtgctcaac ccacagcacg ctgtactggt ctttatcgag tccgggcatc agaacccgct
     1681 cgaaactcgc catcagcttt tcgcgctggc cgggcggtaa atcctgttcg gcaaaagaca
     1741 gaacgccgga agtgtacttt ttggcgtagg gcgaggcgtc gataagctcc cggacttcat
     1801 ccggcttccc ctgcagaacg ctggcgccgt cgcgctggcg atctttaccc agcagataat
     1861 cgaccggccc gccgccgccg ccgcgccccc gaggatgaaa cttaacgatc atcatcagca
     1921 cccttttcca gtacggcatg ccgcaaccgc tcgagtccgg catcgatggc catcagcgcg
     1981 gcgacaatct gcacgcggtc gtgtccgctt ccaccaccgg cgttaacctg gcgggcaatc
     2041 tggttaaggt tgttccccat gccggcaagc tgacgaagca gcgccggcga gatcgacgga
     2101 agtttgccgg cgcgtgcggg cttctcgtcc aggcacgtct gccgcatcca ggccgcgagt
     2161 tgcttaccct cgcagcgctc cagcagacgc cggtgctcgt cctcagtcac ccacatggtg
     2221 agcattttgc tgcgtttgtc tgccaatccg gcctcctgat aacgccggga cggacgggaa
     2281 atcttttccc ggtcggcacg gtgttggaca agccgcaggc gcggcagtgg ttttagcggg
     2341 tttcggggcg cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggctaaaca
     2401 tgttatgacg gagtggcaat ttatgaaagt gcttcatgtg acaggggaaa aatgcggctg
     2461 ccgggcgtaa gtgcaaaatg caggtggtga tgaaatgacg cctcctcgct cactcggtcg
     2521 ctacgctcct gccgtgagac tgcggcgggc gttaccagct cacagattac gggataagca
     2581 aatgccgctc gaagcaggaa aggccaagaa aaggatgcgg agttgccgtt tttccatagg
     2641 ctccgccccc ctgacgagta tcacgaaaat gacgctcaaa tcagcggtgg cgaaacccga
     2701 tgcggcttaa agataccagg cgtttccccc tggtggctcc ctcgtgctcc cccgcattcc
     2761 tgcctttcgg tttaccggtg tcattccgct gttatggccg cggtttctca ttccacgcct
     2821 gaaactcggt tccgggtagg cagttcgctc caggatggac tgtatgcacg aaccccccgt
     2881 tcagtccgac taccacgccc gttccggtaa ctatcaactt gagtccaacc cggaaagaca
     2941 cgacaaatcg ccagtggcgg tagccattgg taactgggtt cacggagcag agattgtaga
     3001 gaatgcctac agagttcttg aagtgaaagc ctgagtgcgg ctacactgaa aggacagttt
     3061 aggtatctgc gctccgtgaa agccagttac catcggaaca aagagttggt agctctgaat
     3121 tccggcaaac gaaccaccgt tggtagcggt ggtttttttg tttacaggga aaaagattac
     3181 gcgcagaaag aaaggatctc aaggaaatcc ttttgctttg gcc
//
