LOCUS       NZ_CP141803            11970 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain Kp2092 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP141803
VERSION     NZ_CP141803.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN35788525
            Assembly: GCF_035621575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Yang,Q., Wang,M. and Yu,Y.
  TITLE     the carbapenem resistant Klebsiella pneumoniae strain isloated from
            hospital samples
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Yang,Q., Wang,M. and Yu,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-DEC-2023) College of Resources and Environment, Fujian
            Agriculture and Forestry University, Shang Xia Dian Road, Fuzhou
            350007, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycle v. v.0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 413.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/15/2024 14:02:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,605
            CDSs (total)                      :: 5,481
            Genes (coding)                    :: 5,361
            CDSs (with protein)               :: 5,361
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 43 of 120
            Pseudo Genes (incomplete)         :: 81 of 120
            Pseudo Genes (internal stop)      :: 11 of 120
            Pseudo Genes (multiple problems)  :: 15 of 120
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141803.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp2092"
                     /isolation_source="sputamentum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="China"
                     /collection_date="2021-03-05"
     gene            join(11682..11970,1..77)
                     /locus_tag="VB123_RS27960"
                     /old_locus_tag="VB123_27960"
     CDS             join(11682..11970,1..77)
                     /locus_tag="VB123_RS27960"
                     /old_locus_tag="VB123_27960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            252..530
                     /locus_tag="VB123_RS27965"
                     /old_locus_tag="VB123_27965"
     CDS             252..530
                     /locus_tag="VB123_RS27965"
                     /old_locus_tag="VB123_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            518..832
                     /locus_tag="VB123_RS27970"
                     /old_locus_tag="VB123_27970"
     CDS             518..832
                     /locus_tag="VB123_RS27970"
                     /old_locus_tag="VB123_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            996..1424
                     /locus_tag="VB123_RS27975"
                     /old_locus_tag="VB123_27975"
     CDS             996..1424
                     /locus_tag="VB123_RS27975"
                     /old_locus_tag="VB123_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1450..1629
                     /locus_tag="VB123_RS27980"
                     /old_locus_tag="VB123_27980"
     CDS             1450..1629
                     /locus_tag="VB123_RS27980"
                     /old_locus_tag="VB123_27980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1656..2186)
                     /locus_tag="VB123_RS27985"
                     /old_locus_tag="VB123_27985"
     CDS             complement(1656..2186)
                     /locus_tag="VB123_RS27985"
                     /old_locus_tag="VB123_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2193..2924)
                     /gene="mobC"
                     /locus_tag="VB123_RS27990"
                     /old_locus_tag="VB123_27990"
     CDS             complement(2193..2924)
                     /gene="mobC"
                     /locus_tag="VB123_RS27990"
                     /old_locus_tag="VB123_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2924..4888)
                     /locus_tag="VB123_RS27995"
                     /old_locus_tag="VB123_27995"
     CDS             complement(2924..4888)
                     /locus_tag="VB123_RS27995"
                     /old_locus_tag="VB123_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6372..6521)
                     /locus_tag="VB123_RS28000"
                     /old_locus_tag="VB123_28000"
     CDS             complement(6372..6521)
                     /locus_tag="VB123_RS28000"
                     /old_locus_tag="VB123_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(6606..6863)
                     /locus_tag="VB123_RS28005"
                     /old_locus_tag="VB123_28005"
     CDS             complement(6606..6863)
                     /locus_tag="VB123_RS28005"
                     /old_locus_tag="VB123_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(6873..8558)
                     /locus_tag="VB123_RS28010"
                     /old_locus_tag="VB123_28010"
     CDS             complement(6873..8558)
                     /locus_tag="VB123_RS28010"
                     /old_locus_tag="VB123_28010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(8663..10150)
                     /gene="ltrA"
                     /locus_tag="VB123_RS28015"
                     /old_locus_tag="VB123_28015"
     CDS             complement(8663..10150)
                     /gene="ltrA"
                     /locus_tag="VB123_RS28015"
                     /old_locus_tag="VB123_28015"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            10795..11040
                     /locus_tag="VB123_RS28020"
                     /old_locus_tag="VB123_28020"
     CDS             10795..11040
                     /locus_tag="VB123_RS28020"
                     /old_locus_tag="VB123_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            11314..11685
                     /gene="tolA"
                     /locus_tag="VB123_RS28025"
                     /old_locus_tag="VB123_28025"
     CDS             11314..11685
                     /gene="tolA"
                     /locus_tag="VB123_RS28025"
                     /old_locus_tag="VB123_28025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     BGC             1873..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5691..6269
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2197..2924)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gtctgaaaaa tgacggctta caggatccgc gaccagtcac cacccaaaag ctcaatgatg
       61 atggttgccg ccagtaacag gtcctttaac ccgcaggctg caatagtcct ggatgatgag
      121 tgagcctgta gcagccctga ttagtggaag cgaagctgaa aaatgggcag caaaagccgt
      181 atttctggca aatagcgccg ccacggatta aagtagtcat gtgtaataca tatgactaca
      241 aaggggcttt tatgtcatca gcaacatcca tcaggctcga tgaagaactt aaggaccggc
      301 tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc gctaatgctg gaggcaatca
      361 ccgaatacat cgaccgggaa gagaagcgca gccagtacct gcgggacgga caggccgcat
      421 ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga agaagcggaa gcctggataa
      481 gtacctgggg aactgagaac gagcaggatg ctccgccatg ccacaggtaa cgatttccgc
      541 gctggcacag cgcgatttac aacgccttca ggactttctc aaaaccaaaa atcggctggc
      601 agccagaaaa ggaggtgagg tgatcgtccg ggctatccag caactgaaaa cattgccaga
      661 cattggccgc ccggtgccgt ttctgccgct ggaatataag gaactggtga tcgggtttgg
      721 cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa atggaccaga ttgtgatcgt
      781 caccgtcagg catcagaaag aatccgggta tccgggggca gacagcctct aataaccgat
      841 gacgggggcg caattcattg cgtggccgtc gccggtttga caagcgcagc gcgtcagaaa
      901 accacatcct gaacggaaaa acgggtaaaa ttcagcgata catttaccgt gagaaattac
      961 acacctttca cacccgataa aatcaggggt atacagtggc gaaaacattg ctcaaccttt
     1021 cacaggcagc acaagccgcg ggcattacca gaagaacgct atacaatcat gtaaaacagg
     1081 gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt cgtggatgtg tcagaactga
     1141 tccgtgtata cggaaatgtg aatatccctg agaaacaaat tcccggcatt tcacaccgtg
     1201 aaaatacaca aaaaaatttc ccacaggaac agcttctggc aatgcaaaag gaactggccg
     1261 atctccggca ggctgtcacc ctgatgctgg aagataaaac atcccgggaa gaagaacgcc
     1321 gccagcacga tgatgagcgc cggaaactcc aggccgaagt ggacagattg acaacagaac
     1381 tgacacagaa gaaaaaacgc ttctggtccg gctggtttag ctgagaactt accgaaggga
     1441 atggagaaca tgacgaagca ggaaaaaact gccctcaata tggctaaatt cattaaggat
     1501 cagtcgctgc tgttgctgga aaagctgaac gagctggaca tggatacccc ggccgacatg
     1561 tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg tgatgcggga taagctcggg
     1621 gaagaataac agcaccggcc atgccggtgc tggcgttatt ttttgccgaa cggccaccac
     1681 cggcgggcgg gtttgtcctc cggcagttca ggcaccggca cgttaaaata gcggctgtta
     1741 tattcccgca tcagctccgc tttcagcttc tcgttcaccg cgctttcccc gtgccgctcg
     1801 ctgaccacct caatcatcgc gttaatctgc tccggtgagc acttcccggc atcaaccacc
     1861 tgcacaccgt tctcgatggc gaaccgctcc acctttcgat agaggtaatc acacagcttc
     1921 attcctgtgg gtttgtcccg ttcccattgc ggattttcac cggtgaaaaa gtacgtggct
     1981 ttttcgtcgc tgttgagccg ctcgtcatac atcccgatgg atttcatcat cgccagatgc
     2041 tgggcccggt cgacaaaaca gaaaacatac gccccggtct gccaggcgag caccaccatg
     2101 tcttccagat gctcgctggt tggcataatg cagcgctcgc cgtctttgtc ttccacgtcc
     2161 tcaataaata ccatgtacag cgccataatc cctcactccg ttgtcataaa tttaaagcac
     2221 gcgaacagcc tctcccggtc cagctccacc gttttgcggg cctcgccggt ctgtggattc
     2281 acgatttcat ccaccgccag aatcgcccgc tgtacccgga caagcgtcgg ccggtcaggg
     2341 cagatgtacc agatttcatc gatgccgttc gcccggcggt tgaacagata cccggacacc
     2401 acttcctgat accgcctgcg gctcttgacg gtgcgctcta cctcaaatgc caccgttctg
     2461 ccgtccagct ccgccagcgc gtccggcact ttcatccctt tcagggccat acggtgcagg
     2521 taccgccagc cggtggcccc ccggccctcc atggagaggc gcagggactg cacggccagc
     2581 cggtgcggca gctgcgccgc cgacaccctt gacggctgaa acggaataac gtccgtcagc
     2641 ggctcgtcct cgtcgaacga gaacgccacc ccgtgcggcg tcagccccca cacgctgacc
     2701 tgacggccgt caaccgggta tttcgcccgg accagcagcc ctttcgccac cagactgtcc
     2761 aggaagcggt aaaccggctg taccgccgca atccccagcc gtcgcgcaac cagctccgcc
     2821 gtggaacaca gctcttcccg cagccagcgc agggtcagcc gtgttttctc cgcgttccgc
     2881 gcctgccgct tcgcgtggct gacgttgtac tgctccagtg tcatcagtaa agccccgcaa
     2941 aatcatcatc cgcttttggt ccgccgccgt cggccggagg caggtactcc gccagcgggt
     3001 ccgttttcgc cagcaccggg aaggttttca gcgaaatttc ccggcgctca acccgcagcg
     3061 ggctgctgaa cgccagccgc gccacgccca gcccggtcat caccgcgaac ccgtcaggca
     3121 ggtgttgcag ggtgttcacg tcaaacagcg gccgcgcctc cttctggatg aagctgtcgc
     3181 cgctgatgtg ctccacgttc cccgcctccc ggctggcccg gcggcgctcc acgtccaccc
     3241 ggatctcccc ggtctggccc gccgcccact gcgcggactc ctggctggcc gcgcgataga
     3301 accagcggat cggcgtgtta tccagtaccg tggttttcac aaaatcagcg ggcagatcct
     3361 gcccgcactg accgaagtcg cccagcgact ggtgcgcgag cagcaggttg cagtcccggc
     3421 tgcgcagcgt ccccagcgcg ttcagcacgt acttcgacag cagatattta atctcatcga
     3481 gcatgatgct cgcgtgcggc cacctccgga actcatcccg cgcaatgatg atttgcgcgc
     3541 agcgggcaaa cagcatcttc tgcacccgga tcaccgcctc atcgtccatc gagccgatga
     3601 cgtacaggca gccgccgccg ttgattatcc ccgccacatc gataccgctg tccgtctgca
     3661 ggacgctcag gtcggccacg ttctccagct tggtgatcag ccccttcacg tcctttttca
     3721 gggcctccgg caacgcgtac gccgccgcca gcacctgctg aatgtttgcc ccctgcggga
     3781 actgctccgc aatcagcttc gccgctttct gctcgctgat gcggtagtgg tccgccacgt
     3841 cgccggtctc actcaggtta aacccggcaa ccagcagatt cttcagggcg tactggtcga
     3901 gatcgcggaa cagattcagc tgcgccggtt tccccggccg cagatccagc agggtgaagt
     3961 ccgggcagtg cgcccggaag acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca
     4021 ccacagcgtc cccgaaatcg cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct
     4081 tgccgctccc gggcaggccg aggatctgaa tattggtctt gcgccaggta tcacgaggga
     4141 ccagcaccgg ctggccctgc tcatccttac ccagaaacac cgcctgctgt ttccgggcct
     4201 gctccgtgga aaaatcggcc agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc
     4261 ggacgtctgt catttctccg gtccgctggc tgctgcgccg gctctcctga ttcagtttcg
     4321 cgttcaccgc ctcgtcagcg gccgcaaaat gaccatcaag cagcaggaac gccagcagcc
     4381 gcgccagaag gacgccggcg acaagccctc cggcacacca ggctatcccg aacaggttag
     4441 ccggctgcag caggtaataa tctgcgttcg catagaggtc cgggcgcagg acggtttctc
     4501 cccagctctc gataaacacg gcgacaacca gcccccccag aagggccaga ccggcagcgc
     4561 cggtcagcca gtacgcgtag ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg
     4621 aatccagcga ggccagcacc gaaaatggca cagtccggtt attatcgcgc ttatagcgat
     4681 cggcatactc ccgccgcgcc ttatggctca gccccacaaa acgcattcgg ccggcccaga
     4741 gttcagccag accgaaaaac aggccgataa ccagaaaaaa accggccaga ctgtaatact
     4801 cccggtacag ccagctgacc cacgggtaaa tctgccgggc cagctccggg atatgccact
     4861 gccaggcaag cgaatccgta ttaaacatgc aatctctctc cttcagacga cgggtttacc
     4921 ggcgtgatgc gcagcccccg ctgcacaagc cgcggacatg ccccaaggca tggcggcggc
     4981 ggtcacgtcc gtggtgattg cgttcagctt tcaccgcgga ggccagcctc tttccgcccc
     5041 atgcggaaaa ggcgcagccc cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa
     5101 ggcggcaccc cagtgcggtc ttttccggtg ttccggcccc cgtcagagtt cgccatagcg
     5161 agcggctgac cacccgcaaa atcaggagca gctttgctgc ttcgtcaggt gcgtccagca
     5221 ccgtggattt ttgtgggtgg tcaggcgtga gcctgacggg ggccgggtga atttctggcg
     5281 tggccggcgt ccagccggac agagccagaa agaggggaaa agaccgtgcg tcggtggcgt
     5341 ccagccagcg taagcaaggt cttttcccct gaagtcagcc acgccccagc ggggatgact
     5401 tgcatttctc cgcaaggaga aaaaagcaac agagataggt taacgtaaaa tgttcagcga
     5461 aaaaccgaca tggttcacct atcctgataa ttgatcgtca gggaatagaa agacttaatc
     5521 aggggacaat atcccacatc agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg
     5581 cgcctcctcg ctcactcggt cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga
     5641 cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg tgaggcaaaa
     5701 cggcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca gagttcacat
     5761 aaacagacgc tttttcggtg catctgtggg agccgtgagg ctcaaccatg aatctgacag
     5821 tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggtaggt cgctccctct
     5881 tgcgctctcc tgttccgacc ctgccgttta ccggatacct gtccgccgtt ctcccttgcg
     5941 ggaagtgtgg cgctttctca tagctcacac actggtatct cggctcggtg taggtcgttc
     6001 gctccaagct gggctgtaag caagaactcc ccgttcagcc cgactgctgc gccttatccg
     6061 gtaactgttc acttgagtcc aacccgggaa agcacgcaaa acgccactgg cagcagccat
     6121 tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct cttgaagtgt
     6181 gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg cgaaagccag
     6241 ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac ctccccaggt
     6301 ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc aagaagatcc
     6361 tttgatcttt tctactgaac cgcgatcccc gtcagttcag aggaggacga tggtgccacc
     6421 gttccgccct gaacatcccg gatataattt gcctgacatg cggccagaag aaagccagca
     6481 acaatcaata taaaaagaaa aatagtcttt acctttttca tatactttcc ctgtatttaa
     6541 agactccggc attcatccct gaacaccgga agtcagtatt tcaaatcatc cttgataatt
     6601 ttttgttacc agttaccgtc ccgataatca aaagctatat aacaattatc cacatcaggt
     6661 tttatttgat ttttaaaaaa tggctgtagt aaaggaaccc attctttctt caaatcaaac
     6721 tcaccattat taatattatc ctttaaaggc attcccaagc tttcaatgac agaaccatcg
     6781 tcaccaaagt ctttagaata ctcttcccct ttaaactctt cagttttctt atcaaaccat
     6841 gtcaaacgta atttaagccc catatttacc tctcaaagat attttttaat gttccgtttt
     6901 ggatctggcc ctttaacctg cttacccgtt ttgggatcaa atgcgcctag gtgttcgcca
     6961 tcactggcac gatacccttc aagctcaccg tgctgggaat cccattcata aattttacgt
     7021 tttttatcgc cataccatcg ggcccgctta ccaccaccac cttgtttagg tgttttagga
     7081 tcacctcgct ttaactctcc aagtcccttt atgtcttctg ttttaggtac tggatgataa
     7141 tcatggccgt agtctttagt ccccttgcga ggcttgttct tttcctcatt taacttattt
     7201 tctgcatcct tcgctttttt ctccttttgt ttccggctct ccatcgcccc ggtaagtgca
     7261 gccttatctc tggataagga ttcattcatt gcgtttaaag atgcctgagc cgcttttaat
     7321 ttctcattct gattctgaat atccttatca agatagcgaa cctgatcctg ccacccggag
     7381 ccatattcat gaggcgggtt ataagtcaca aaatcatctt tatttgcctc tgcctctttt
     7441 acctttttat cggcttcctg aacagcagcc cttgcaccgg ggagcgcctt ctctgtcgat
     7501 gcaatgcggc tgtttaaggt cgcaatattt ttatcttcgg catccagctc agctttcgct
     7561 ttatcatagt ctcttttcag cccctcttcc ggatgagcgg cgtcccatgc ctgctggcga
     7621 cgcttttctt cctcctgacg ctgttttacc tgtgccgggg taagaacatc tgtcaccgac
     7681 acataaaccg gcttctgtcc ggtctctttc gggaaacgaa taacggcctc acgggagtta
     7741 ccaccggctg taaaaccagc cggacgtgaa tcaccttttt cagcaacaat gccttttggc
     7801 ggggctttcg ctgtcggaac acctttcggt acgctcacct gcagagacgg gagacccgga
     7861 atcgacacac tgaatacccc cggacgtttt gtcggtttcg catccaccac agggacactc
     7921 attggccgac ctgcgacaac cgcaatatgc tgccgctcat ccttcaccac atccacaacc
     7981 cgttgtctga cacgaaccgt cgcctggtcc agtggtaaag tacttgccgg agtctccgtc
     8041 accgcatcgg ccggtaatga cgtcataatt tttgacatca ttttcggatc atcttttgca
     8101 atctcagaag gcagcacacc gtagatcgca atcccccaca gaccaaactt aaacggcccc
     8161 ttcagggcag ccagcacatc agcaacggcg gccgacaacg catcgccgga aactgataaa
     8221 gccagtcctt cagcccccgg agtagccagg gcaggcagac cgaaggccat cgcagtggcc
     8281 gaagactgtc cgccaccaga gctaccgcta tgattgccgc tgttaccacc attaccaccg
     8341 ggtttactcc cgccgttacc aaactccccc ggattatagt tatgaatatc tccattcggc
     8401 gtatgcgtcg taccccagcc cggcccgctg tttggatccg taccaccacc tgatgaaggg
     8461 ccacctttac cagacgtacc gttcacattc ccactggcct gaccaccatt atgtcccaga
     8521 cctgaattac ccggacctcg accgtctcca ccactcatac cgttttcctc tttgacattt
     8581 aaaacaataa attaaagcca acatgtcgcg gtagggatac ccgttaccgg ataccccccg
     8641 cacagatccc ggcgtgcgcg atttacgcac cgggctcctg cctcgggtgt ctggcggtga
     8701 accgctccac aggccatgga tgaagaaccc gaacccttgg tagccatgcg gctgccagtt
     8761 tgtttgcttt cgtccaggtc gtatcatcct tctggctcct gcgcctgagc gcccggcgcc
     8821 agaggtttgt tacgtgtgtc ctgaacttct gcatggtggg gaagttgccc ggtaccgagt
     8881 gatagttcag gtatccctga accactctcc tgagccattt tccctgttcg gggattgagt
     8941 aatgccagcg ccttcgcaga ccgtctttga tggctttcag agttgccgtc atccgatccc
     9001 ggcgggtctt tcgtatcagc atgaacctgc cgttgcgatc tttcccgctg atgtgcgtga
     9061 acccgaggaa gttgaacgtt tctggtttgc cttttcccct gatggcacgg ttttcggcag
     9121 cgaagcggcc gaactccatc agacgggttt tctccgggtg aaccgtgagt ccgaactccc
     9181 tcagtctgcg ctgcatggct atacggaagc gccgggcatc gtatcgtttg tcgaacccga
     9241 tgacgatgtc atcggcgtat ctgaccatta ccacattgcc tgtggcatag cgacgtcgcc
     9301 actgatgcgc ccacagatcg aagacgtagt ggaggtatat gtttgccagc agcggtgaga
     9361 tgaccgcacc ctgtggggtg ccttcctccg ttgctcgcca ttgaccctcc tccgacgtcc
     9421 cggctgtgag ccacttacgt atgagcctga ttaccctccg gtcgccgatc cgatgctctg
     9481 tgaacctgat cagccattcg tggctcaccc tgtcgaagaa ctgactgatg tcggcatcca
     9541 gtacccagtt tacgttagtg cgtaccagcc ctgtggccag tgcgtccagt gcatcgtgct
     9601 ggcttcgccc gggtctgaac ccgtatgaga accccataaa gtcgttttca tagactgcgt
     9661 tcaggatttt caccagcgca tactggacga tcttgtcctc cagcgaggcg atgccgagcg
     9721 ggcgttgttt tccatccgct tttgggatgt agtgacgcct gccgggctgc gccctgtagc
     9781 tgccctgatg tagcctccgg tgcagatctg ttatgttgtt cttcatgttt ccggcgtagt
     9841 ccatccacct gatgccatcc actccggcgg ccgctttcct gctcagggag aggaatgcgg
     9901 cttccagtgc ttcgactgtc agcaggtgga acaatgctgt aaaccgttct ttcttccgct
     9961 gcttcgcagc ttcccgcacg cgtgacagcc tctgtgacat gctttcccgg ctctgtgtcc
    10021 ggcgcatgtg tggctgttcc gcgttcccct tggccccgct ccttcgctcc actgactccg
    10081 ctcctttcgg gttgttcgcc tgcttcgccg ctactatgag cgagtccgac ttctcctctc
    10141 cgtacatcac cggctatgac tcctcgtctt cccggtgcgg gccatctccg acactggcag
    10201 atggtcagag gggagatctc ccggttcccg cgtagagatc gtattgacat gccagggtct
    10261 cagaccccgc cgggtccatg tggcactcgc agtatcgcac cctatgatgt tgccttccgt
    10321 taacagtaca acgtcggcac ccggtaattt aatatacatt tcgtggctca atggctggcc
    10381 tgtcaacacc cctgtcaacg cttcgcccca tacctcgcgg tatgcaacgc atgactcggg
    10441 gaccttgtgg attgctggtc cttcaatggt cggggacttt caccccttga tctctaaccg
    10501 gtctcccggc gcacactgta catatacaca gtataattat taccttactc ctcccgtttt
    10561 caggtgtcaa tatatgagat tagttctcat cttcaggcac aaaaaagcgc ccggagccac
    10621 ctgtaagcag atcgcgtctt ttcccgaaaa tttctcatgg caacaaattt tattatttaa
    10681 gacattcatc acacttacat ttcagatggt atagacacat ctgaaagcta actggcatga
    10741 ggagaggacc tgcggttaaa ctgttcggga aacctgagac gcccggaggc cactatgacg
    10801 cacctgaaac tgactcccgc tgaagaaaag cgcctggcgg acgcctttgt cgctcaggca
    10861 ctggccatgg gcctgaaagg gaacaaaacg ctgccaaagg cggtaaaaca acaggtgcgg
    10921 gaaagggtgc ttcgtgccat tcaggacgaa cgtgaagcca ggcgggcgca ggaaatgcgt
    10981 cgggagaaat acgaggctac ggaaaccacc ttcacctggc agccacagcg ccgccggtaa
    11041 ggggtattta ccataacgtc gcattgcccg caccgctcag acggtctgag ggcgtttccg
    11101 gcggctacgg gataaaacag gcaacaaacc acccggaaaa ccgccggaaa cgcgcccggt
    11161 aaattgtaac ccgctgcatg cctatgcacg caactgcatg gcgggccgtt tggtgtaaaa
    11221 acgatacgat aagccgtgtt tatgtaaatg aacgtggccg taaagagtga caaatataca
    11281 tgtaacattt tttactgaca ggtcatcaac ccgatgcgtt taataccctt tatcctgctg
    11341 gcattgtgca gccccttact cctgacggcc tgtaagcatt ccaccaccca gccgccgaaa
    11401 atttatactc tggataagtc atctaaaatc agtagctacg ctgcggccat caagaccgct
    11461 gttgaaatgc agatgtttga accagaggcc tatgcgggaa aaatgtgcac cgtgcggata
    11521 tccatacaac gtgatggcac tctgaacagc gccattgctg aaggaggaga tccgaaactc
    11581 tgtaaagcag caatatcagc cgttacccac gccaaaattc cgccggcacc ggatgaaaat
    11641 acctggcaaa tattcaaaaa cgcccctctg gatttcagac catgaaagct tttactctga
    11701 cagtgctggc ggccatgttc ctcactccgg tacatgcgat accactaaaa cccgtaacca
    11761 cactgcagcc aacctacccg gtacaggccg cagcaaaacg catcgaaggt caggtgaccg
    11821 ccacgtttga tattctggac gatggacgag tggacaacgt gtcgcttacc ggctgtcacc
    11881 ttttttttcg ggaaacgctg acggcaatga ggcactggct atatcccgca gggcacccgg
    11941 caatgcgaca ggttataaat tttgaattca
//
