LOCUS       NZ_CP141741             3071 bp    DNA     circular CON 21-APR-2024
DEFINITION  Escherichia coli strain 2023CK-01509 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP141741
VERSION     NZ_CP141741.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38788848
            Assembly: GCF_034331185.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 3071)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_034331185.1-RS_2024_04_21
            Annotation Date                   :: 04/21/2024 14:22:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,029
            CDSs (total)                      :: 4,907
            Genes (coding)                    :: 4,698
            CDSs (with protein)               :: 4,698
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 209
            CDSs (without protein)            :: 209
            Pseudo Genes (ambiguous residues) :: 0 of 209
            Pseudo Genes (frameshifted)       :: 67 of 209
            Pseudo Genes (incomplete)         :: 129 of 209
            Pseudo Genes (internal stop)      :: 48 of 209
            Pseudo Genes (multiple problems)  :: 34 of 209
            Pseudo Genes (short protein)      :: 4 of 209
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141741.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2023CK-01509"
                     /isolation_source="Wound/Abscess"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed5"
                     /geo_loc_name="USA"
                     /collection_date="2023-11-02"
     gene            complement(772..1416)
                     /locus_tag="VLW43_RS25140"
                     /old_locus_tag="VLW43_25140"
     CDS             complement(772..1416)
                     /locus_tag="VLW43_RS25140"
                     /old_locus_tag="VLW43_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017900990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolone resistance pentapeptide repeat protein
                     QnrB19"
                     /protein_id="WP_012954666.1"
                     /translation="MTLALVGEKIDRNRFTGEKVENSTFFNCDFSGADLSGTEFIGCQF
                     YDRESQKGCNFSRAMLKDAIFKSCDLSMADFRNVSALGIEIRHCRAQGADFRGASFMNM
                     ITTRTWFCSAYITNTNLSYANFSKVVLEKCELWENRWMGTQVLGATFSGSDLSGGEFST
                     FDWRAANFTHCDLTNSELGDLDIRGVDLQGVKLDSYQASLLMERLGIAVIG"
                     /name="quinolone resistance pentapeptide repeat protein
                     QnrB19"
     gene            complement(1510..>1665)
                     /locus_tag="VLW43_RS25145"
                     /old_locus_tag="VLW43_25145"
                     /pseudo=""
     CDS             complement(1510..>1665)
                     /locus_tag="VLW43_RS25145"
                     /old_locus_tag="VLW43_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     ARG             complement(776..1416)
                     /name="qnrB19"
                     /gene="qnrB19"
                     /gene_name="quinolone resistance pentapeptide repeat
                     protein QnrB19"
                     /tool="amrfinderplus"
                     /drug_class="QUINOLONE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          2395..2568
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1925..2416)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.51"
                     /coverage="81.0"
                     /evalue="0.0"
ORIGIN
        1 ctccttcagt tcatacatca cttttacaac acttttacaa cactccttcg gcctgtacat
       61 cacttttaca acacttttgc atcactttta catcatttat acatcacttt tacatcattc
      121 cttcagctca tacgtcactt tcatcctctg tgccatttgt acatcacccc cttcctgaac
      181 gtgctgacag cgggttttcc ggctgcgccg gcaacgcttc acggccacgc aataaattgc
      241 gtccccgttc accgttttcg gatgggaaga acgccggaaa caggacttcc cctggctgaa
      301 tggtgtgaat ttccgcgcta tgcacttgcg cgcatactca tgcatgccgt aaaaacagag
      361 cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct gttttaccgg tttcccgtca
      421 gaaacgccct cagagcctct caggcggtgc acgcaaggga tgttatgttt aatcggtctg
      481 accgtcaggt acaaaaaaaa gcccttaggg ggcttttttt ttgctatgag ttagccatat
      541 tttcgttaag acatggcttc atgattcggg agtggttatt cgttgttctc gcgaaaatga
      601 attggttaga tattcatcaa attcctgtcc agaatgcagc attagatggc atgtcgttga
      661 tgcggtaggt caggatattc gtccactgga gaaaacgttt cacaatgctt gccaggagtc
      721 gtactggtta aaagtaaatc gtcaaacgca tctcccggtg taaacgctga actaaccaat
      781 cacagcgatg ccaagccgct ccatgagcaa cgatgcctgg tagctgtcta acttaacgcc
      841 ttgtaaatca acaccccgaa tatctaagtc acctaactcc gaattggtca gatcgcaatg
      901 tgtgaagttt gctgctcgcc agtcgaaagt cgaaaactcg ccgccggaga gatctgaacc
      961 actgaacgtc gcacccagta cctgagtccc catccagcgg ttttcccaca gctcacactt
     1021 ttccaacacg actttcgaaa aattggcgta gcttagatta gtattagtga tatatgcgct
     1081 gcaaaaccag gtgcgcgtgg tgatcatatt cataaagctt gcaccgcgga aatctgcgcc
     1141 ttgtgcgcgg cagtggcgaa tttcaatgcc caaggcactg acgttgcgga aatctgccat
     1201 tgataaatca cagcttttga aaatggcatc tttcagcatt gcgcgactaa aattgcaccc
     1261 tttctgactt tcgcgatcat agaactggca gccgataaat tcagtgccgc tcaggtcggc
     1321 acctgaaaaa tcgcagttaa aaaatgtact attttcaact ttctcaccgg tgaagcgatt
     1381 tctgtcaatt ttttcgccaa ctaatgccag agtcatatct atgcctgtgt ttttatacag
     1441 taatgacgcc atggtaaacc ttatgacgtt atgcgtcaaa cccgccagta tgacacctgc
     1501 aaatgcgtat tagatctggt gtttcttcag caaggcacgt agctgatgat aagtcagacc
     1561 cagtaattca gcggcttttt tctggttaaa tttagcctgc tgtaagctgg tttgtagaaa
     1621 gtctttctct tgctgctgct ggaattcacg cagatccagc gttataacga tgtgtatcct
     1681 atgtgctcgg attggataga tttccattac ggtgaaggat cttcttgaga tcctttctgt
     1741 ggcgtcgtaa tctcttgctc tgtaaacgaa aaaaccgccc tgcaaggcgg tttttcgaag
     1801 gttctcagag cagcaactct ttgaaccgtg gtaactggct tacgaggagc gcagtaacca
     1861 aatctgttct ttcagtttag ccttaacagg cgcataactt caagactaac tcctctaaat
     1921 cagttaccag tggctgctgc cagtggcgat aagtcgtgtc tttccgggtt ggactcaaga
     1981 tgatagttac cggataaggc gcagcagtcg gactgaacgg ggggttcgtg catacagtcc
     2041 atccaggagc gaactgcctt cgcggaactg agtgtcaggc gtggaatgag ataaacgcgg
     2101 ccataacagc ggaatgacac cggtaaaccg aaaggcagga atgcggggga gcacgaggga
     2161 gccaccaggg ggaaacgcct ggtatcttta agccgcatcg ggtttcgcca ccactgattt
     2221 gagcgtcaga tttcatgatg tttgtcaggg gggcggagcc tatggaaaaa cggcttcgcc
     2281 gcggccttat ctcttccctg ttaagctcct tcctggtatc tcccaggaaa tttccgcccc
     2341 gcctgtaagc cggcaccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2401 aggaagcgga atatatcctg tactacatat cctgctgacg cgctggtgcc gccttttttc
     2461 tcctgctaca tgaagcactt cactgatttc cacatccgtg ccaacatagt cagccagtat
     2521 acactccgct agcgctacgt gactggttca gggctgcgcc ccgaaacccg ctaacaacct
     2581 ctctgctact ggatatcacc ccatgccatc tgaatatgat gaacaacggg gttcgactga
     2641 tacgcagctt cccggcgaat atgacctttt tgatttaatg gcgaaggtta ctgaagagat
     2701 cctgcatgcc cctgtggatt ctgggccgcc tgtcggccgg gaagcgttat agctttttcg
     2761 cttgcccgcg tacgtggtaa tacgccggta ttacctttta tttctttatt ttcatgtaat
     2821 tacagtgggt ttttaatacc tgggtattac tcgtgaaaac gaaacattac ttttttttct
     2881 tttaattcat gcagttgcga tgaaaatcgc ggctgcgtta ggtgtatgac cattttgagg
     2941 ttgaaaaata atcaaccatg gcgggtgatt tacgcaggtt aaggggagat cgtgacaaaa
     3001 aaaagcgttc ctcactgtat cgttcatgcc tgcatgaacg ggttattctt cctgactaca
     3061 gaattacatc g
//
