LOCUS       NZ_CP141670            11970 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KPN1023 plasmid pK1023_4, complete
            sequence.
ACCESSION   NZ_CP141670
VERSION     NZ_CP141670.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38931008
            Assembly: GCF_035620095.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Zhou,L.
  TITLE     Genomic of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Zhou,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-DEC-2023) Anyida, Anyida, Furonglu, Heifeu 230601,
            China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 27-DEC-2021
            Assembly Method                   :: flye v. v2.9.1; flye v. v2.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 201.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore ONT
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/13/2024 23:16:08
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,948
            CDSs (total)                      :: 5,827
            Genes (coding)                    :: 5,638
            CDSs (with protein)               :: 5,638
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 189
            CDSs (without protein)            :: 189
            Pseudo Genes (ambiguous residues) :: 0 of 189
            Pseudo Genes (frameshifted)       :: 70 of 189
            Pseudo Genes (incomplete)         :: 126 of 189
            Pseudo Genes (internal stop)      :: 25 of 189
            Pseudo Genes (multiple problems)  :: 32 of 189
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141670.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN1023"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pK1023_4"
                     /geo_loc_name="China:Hefei"
                     /collection_date="2021-12-27"
     gene            join(10007..11970,1)
                     /locus_tag="U8229_RS29620"
                     /old_locus_tag="U8229_29620"
     CDS             join(10007..11970,1)
                     /locus_tag="U8229_RS29620"
                     /old_locus_tag="U8229_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1..732
                     /gene="mobC"
                     /locus_tag="U8229_RS29625"
                     /old_locus_tag="U8229_29625"
     CDS             1..732
                     /gene="mobC"
                     /locus_tag="U8229_RS29625"
                     /old_locus_tag="U8229_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            739..1269
                     /locus_tag="U8229_RS29630"
                     /old_locus_tag="U8229_29630"
     CDS             739..1269
                     /locus_tag="U8229_RS29630"
                     /old_locus_tag="U8229_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1296..1475)
                     /locus_tag="U8229_RS29635"
                     /old_locus_tag="U8229_29635"
     CDS             complement(1296..1475)
                     /locus_tag="U8229_RS29635"
                     /old_locus_tag="U8229_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1501..1929)
                     /locus_tag="U8229_RS29640"
                     /old_locus_tag="U8229_29640"
     CDS             complement(1501..1929)
                     /locus_tag="U8229_RS29640"
                     /old_locus_tag="U8229_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(2093..2407)
                     /locus_tag="U8229_RS29645"
                     /old_locus_tag="U8229_29645"
     CDS             complement(2093..2407)
                     /locus_tag="U8229_RS29645"
                     /old_locus_tag="U8229_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2395..2673)
                     /locus_tag="U8229_RS29650"
                     /old_locus_tag="U8229_29650"
     CDS             complement(2395..2673)
                     /locus_tag="U8229_RS29650"
                     /old_locus_tag="U8229_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(2848..3213)
                     /locus_tag="U8229_RS29655"
                     /old_locus_tag="U8229_29655"
     CDS             complement(2848..3213)
                     /locus_tag="U8229_RS29655"
                     /old_locus_tag="U8229_29655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(3210..3581)
                     /gene="tolA"
                     /locus_tag="U8229_RS29660"
                     /old_locus_tag="U8229_29660"
     CDS             complement(3210..3581)
                     /gene="tolA"
                     /locus_tag="U8229_RS29660"
                     /old_locus_tag="U8229_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(3855..4100)
                     /locus_tag="U8229_RS29665"
                     /old_locus_tag="U8229_29665"
     CDS             complement(3855..4100)
                     /locus_tag="U8229_RS29665"
                     /old_locus_tag="U8229_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4745..6232
                     /gene="ltrA"
                     /locus_tag="U8229_RS29670"
                     /old_locus_tag="U8229_29670"
     CDS             4745..6232
                     /gene="ltrA"
                     /locus_tag="U8229_RS29670"
                     /old_locus_tag="U8229_29670"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            6337..8022
                     /locus_tag="U8229_RS29675"
                     /old_locus_tag="U8229_29675"
     CDS             6337..8022
                     /locus_tag="U8229_RS29675"
                     /old_locus_tag="U8229_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8032..8289
                     /locus_tag="U8229_RS29680"
                     /old_locus_tag="U8229_29680"
     CDS             8032..8289
                     /locus_tag="U8229_RS29680"
                     /old_locus_tag="U8229_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            8374..8523
                     /locus_tag="U8229_RS29685"
                     /old_locus_tag="U8229_29685"
     CDS             8374..8523
                     /locus_tag="U8229_RS29685"
                     /old_locus_tag="U8229_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             1337..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(8627..9205)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2..729
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgacactgg agcagtacaa cgtcagccac gcgaagcggc aggcgcggaa cgcggagaaa
       61 acacggctga ccctgcgctg gctgcgggaa gagctgtgtt ccacggcgga gctggttgcg
      121 cgacggctgg ggattgcggc ggtacagccg gtttaccgct tcctggacag tctggtggcg
      181 aaagggctgc tggtccgggc gaaatacccg gttgacggcc gtcaggtcag cgtgtggggg
      241 ctgacgccgc acggggtggc gttctcgttc gacgaggacg agccgctgac ggacgttatt
      301 ccgtttcagc cgtcaagggt gtcggcggcg cagctgccgc accggctggc cgtgcagtcc
      361 ctgcgcctct ccatggaggg ccggggggcc accggctggc ggtacctgca ccgtatggcc
      421 ctgaaaggga tgaaagtgcc ggacgcgctg gcggagctgg acggcagaac ggtggcattt
      481 gaggtagagc gcaccgtcaa gagccgcagg cggtatcagg aagtggtgtc cgggtatctg
      541 ttcaaccgcc gggcgaacgg catcgatgaa atctggtaca tctgccctga ccggccgacg
      601 cttgtccggg tacagcgggc gattctggcg gtggatgaaa tcgtgaatcc acagaccggc
      661 gaggcccgca aaacggtgga gctggaccgg gagaggctgt tcgcgtgctt taaatttatg
      721 acaacggagt gagggattat ggcgctgtac atggtattta ttgaggacgt ggaagacaaa
      781 gacggcgagc gctgcattat gccaaccagc gagcatctgg aagacatggt ggtgctcgcc
      841 tggcagaccg gggcgtatgt tttctgtttt gtcgaccggg cccagcatct ggcgatgatg
      901 aaatccatcg ggatgtatga cgagcggctc aacagcgacg aaaaagccac gtactttttc
      961 accggtgaaa atccgcaatg ggaacgggac aaacccacag gaatgaagct gtgtgattac
     1021 ctctatcgaa aggtggagcg gttcgccatc gagaacggtg tgcaggtggt tgatgccggg
     1081 aagtgctcac cggagcagat taacgcgatg attgaggtgg tcagcgagcg gcacggggaa
     1141 agcgcggtga acgagaagct gaaagcggag ctgatgcggg aatataacag ccgctatttt
     1201 aacgtgccgg tgcctgaact gccggaggac aaacccgccc gccggtggtg gccgttcggc
     1261 aaaaaataac gccagcaccg gcatggccgg tgctgttatt cttccccgag cttatcccgc
     1321 atcacctgcc agagtgtttc cgcatcctcg tgaagccgtt cgcacatgtc ggccggggta
     1381 tccatgtcca gctcgttcag cttttccagc aacagcagcg actgatcctt aatgaattta
     1441 gccatattga gggcagtttt ttcctgcttc gtcatgttct ccattccctt cggtaagttc
     1501 tcagctaaac cagccggacc agaagcgttt tttcttctgt gtcagttctg ttgtcaatct
     1561 gtccacttcg gcctggagtt tccggcgctc atcatcgtgc tggcggcgtt cttcttcccg
     1621 ggatgtttta tcttccagca tcagggtgac agcctgccgg agatcggcca gttccttttg
     1681 cattgccaga agctgttcct gtgggaaatt tttttgtgta ttttcacggt gtgaaatgcc
     1741 gggaatttgt ttctcaggga tattcacatt tccgtataca cggatcagtt ctgacacatc
     1801 cacgacaggg ttgtttttcc cgtcacgtga cacggtaacc tttccctgtt ttacatgatt
     1861 gtatagcgtt cttctggtaa tgcccgcggc ttgtgctgcc tgtgaaaggt tgagcaatgt
     1921 tttcgccact gtatacccct gattttatcg ggtgtgaaag gtgtgtaatt tctcacggta
     1981 aatgtatcgc tgaattttac ccgtttttcc gttcaggatg tggttttctg acgcgctgcg
     2041 cttgtcaaac cggcgacggc cacgcaatga attgcgcccc cgtcatcggt tattagaggc
     2101 tgtctgcccc cggatacccg gattctttct gatgcctgac ggtgacgatc acaatctggt
     2161 ccatttcccg gtcgtggcgg tagagcatca catagccact gtcgccaaac ccgatcacca
     2221 gttccttata ttccagcggc agaaacggca ccgggcggcc aatgtctggc aatgttttca
     2281 gttgctggat agcccggacg atcacctcac ctccttttct ggctgccagc cgatttttgg
     2341 ttttgagaaa gtcctgaagg cgttgtaaat cgcgctgtgc cagcgcggaa atcgttacct
     2401 gtggcatggc ggagcatcct gctcgttctc agttccccag gtacttatcc aggcttccgc
     2461 ttcttccgca gtaaggtgca gacccgtttc ctgatagtgc tgccatgcgg cctgtccgtc
     2521 ccgcaggtac tggctgcgct tctcttcccg gtcgatgtat tcggtgattg cctccagcat
     2581 tagcgcgtgc gctgagcggt gccggtcatc ggccagcgtt ttcagccggt ccttaagttc
     2641 ttcatcgagc ctgatggatg ttgctgatga cataaaagcc cctttgtagt catatgtatt
     2701 acacatgact actttaatcc gtggcggcgc tatttgccag aaatacggct tttgctgccc
     2761 atttttcagc ttcgcttcca ctaatcaggg ctgctacagg ctcactcatc atccaggact
     2821 attgcagcct gcgggttaaa ggacctgtta ctggcggcaa ccatcatcat tgagcttttg
     2881 ggtggtgact ggtcgcggat cctgtaagcc gtcatttttc agactgaatt caaaatttat
     2941 aacctgtcgc attgccgggt gccctgcggg atatagccag tgcctcattg ccgtcagcgt
     3001 ttcccgaaaa aaaaggtgac agccggtaag cgacacgttg tccactcgtc catcgtccag
     3061 aatatcaaac gtggcggtca cctgaccttc gatgcgtttt gctgcggcct gtaccgggta
     3121 ggttggctgc agtgtggtta cgggttttag tggtatcgca tgtaccggag tgaggaacat
     3181 ggccgccagc actgtcagag taaaagcttt catggtctga aatccagagg ggcgtttttg
     3241 aatatttgcc aggtattttc atccggtgcc ggcggaattt tggcgtgggt aacggctgat
     3301 attgctgctt tacagagttt cggatctcct ccttcagcaa tggcgctgtt cagagtgcca
     3361 tcacgttgta tggatatccg cacggtgcac atttttcccg cataggcctc tggttcaaac
     3421 atctgcattt caacagcggt cttgatggcc gcagcgtagc tactgatttt agatgactta
     3481 tccagagtat aaattttcgg cggctgggtg gtggaatgct tacaggccgt caggagtaag
     3541 gggctgcaca atgccagcag gataaagggt attaaacgca tcgggttgat gacctgtcag
     3601 taaaaaatgt tacatgtata tttgtcactc tttacggcca cgttcattta cataaacacg
     3661 gcttatcgta tcgtttttac accaaacggc ccgccatgca gttgcgtgca taggcatgca
     3721 gcgggttaca atttaccggg cgcgtttccg gcggttttcc gggtggtttg ttgcctgttt
     3781 tatcccgtag ccgccggaaa cgccctcaga ccgtctgagc ggtgcgggca atgcgacgtt
     3841 atggtaaata ccccttaccg gcggcgctgt ggctgccagg tgaaggtggt ttccgtagcc
     3901 tcgtatttct cccgacgcat ttcctgcgcc cgcctggctt cacgttcgtc ctgaatggca
     3961 cgaagcaccc tttcccgcac ctgttgtttt accgcctttg gcagcgtttt gttccctttc
     4021 aggcccatgg ccagtgcctg agcgacaaag gcgtccgcca ggcgcttttc ttcagcggga
     4081 gtcagtttca ggtgcgtcat agtggcctcc gggcgtctca ggtttcccga acagtttaac
     4141 cgcaggtcct ctcctcatgc cagttagctt tcagatgtgt ctataccatc tgaaatgtaa
     4201 gtgtgatgaa tgtcttaaat aataaaattt gttgccatga gaaattttcg ggaaaagacg
     4261 cgatctgctt acaggtggct ccgggcgctt ttttgtgcct gaagatgaga actaatctca
     4321 tatattgaca cctgaaaacg ggaggagtaa ggtaataatt atactgtgta tatgtacagt
     4381 gtgcgccggg agaccggtta gagatcaagg ggtgaaagtc cccgaccatt gaaggaccag
     4441 caatccacaa ggtccccgag tcatgcgttg cataccgcga ggtatggggc gaagcgttga
     4501 caggggtgtt gacaggccag ccattgagcc acgaaatgta tattaaatta ccgggtgccg
     4561 acgttgtact gttaacggaa ggcaacatca tagggtgcga cactgcgagt gccacatgga
     4621 cccggcgggg tctgagaccc tggcatgtca atacgatctc tacgcgggaa ccgggagatc
     4681 tcccctctga ccatctgcca gtgtcggaga tggcccgcac cgggaagacg aggagtcata
     4741 gccggtgatg tacggagagg agaagtcgga ctcgctcata gtagcggcga agcaggcgaa
     4801 caacccgaaa ggagcggagt cagtggagcg aaggagcggg gccaagggga acgcggaaca
     4861 gccacacatg cgccggacac agagccggga aagcatgtca cagaggctgt cacgcgtgcg
     4921 ggaagctgcg aagcagcgga agaaagaacg gtttacagca ttgttccacc tgctgacagt
     4981 cgaagcactg gaagccgcat tcctctccct gagcaggaaa gcggccgccg gagtggatgg
     5041 catcaggtgg atggactacg ccggaaacat gaagaacaac ataacagatc tgcaccggag
     5101 gctacatcag ggcagctaca gggcgcagcc cggcaggcgt cactacatcc caaaagcgga
     5161 tggaaaacaa cgcccgctcg gcatcgcctc gctggaggac aagatcgtcc agtatgcgct
     5221 ggtgaaaatc ctgaacgcag tctatgaaaa cgactttatg gggttctcat acgggttcag
     5281 acccgggcga agccagcacg atgcactgga cgcactggcc acagggctgg tacgcactaa
     5341 cgtaaactgg gtactggatg ccgacatcag tcagttcttc gacagggtga gccacgaatg
     5401 gctgatcagg ttcacagagc atcggatcgg cgaccggagg gtaatcaggc tcatacgtaa
     5461 gtggctcaca gccgggacgt cggaggaggg tcaatggcga gcaacggagg aaggcacccc
     5521 acagggtgcg gtcatctcac cgctgctggc aaacatatac ctccactacg tcttcgatct
     5581 gtgggcgcat cagtggcgac gtcgctatgc cacaggcaat gtggtaatgg tcagatacgc
     5641 cgatgacatc gtcatcgggt tcgacaaacg atacgatgcc cggcgcttcc gtatagccat
     5701 gcagcgcaga ctgagggagt tcggactcac ggttcacccg gagaaaaccc gtctgatgga
     5761 gttcggccgc ttcgctgccg aaaaccgtgc catcagggga aaaggcaaac cagaaacgtt
     5821 caacttcctc gggttcacgc acatcagcgg gaaagatcgc aacggcaggt tcatgctgat
     5881 acgaaagacc cgccgggatc ggatgacggc aactctgaaa gccatcaaag acggtctgcg
     5941 aaggcgctgg cattactcaa tccccgaaca gggaaaatgg ctcaggagag tggttcaggg
     6001 atacctgaac tatcactcgg taccgggcaa cttccccacc atgcagaagt tcaggacaca
     6061 cgtaacaaac ctctggcgcc gggcgctcag gcgcaggagc cagaaggatg atacgacctg
     6121 gacgaaagca aacaaactgg cagccgcatg gctaccaagg gttcgggttc ttcatccatg
     6181 gcctgtggag cggttcaccg ccagacaccc gaggcaggag cccggtgcgt aaatcgcgca
     6241 cgccgggatc tgtgcggggg gtatccggta acgggtatcc ctaccgcgac atgttggctt
     6301 taatttattg ttttaaatgt caaagaggaa aacggtatga gtggtggaga cggtcgaggt
     6361 ccgggtaatt caggtctggg acataatggt ggtcaggcca gtgggaatgt gaacggtacg
     6421 tctggtaaag gtggcccttc atcaggtggt ggtacggatc caaacagcgg gccgggctgg
     6481 ggtacgacgc atacgccgaa tggagatatt cataactata atccggggga gtttggtaac
     6541 ggcgggagta aacccggtgg taatggtggt aacagcggca atcatagcgg tagctctggt
     6601 ggcggacagt cttcggccac tgcgatggcc ttcggtctgc ctgccctggc tactccgggg
     6661 gctgaaggac tggctttatc agtttccggc gatgcgttgt cggccgccgt tgctgatgtg
     6721 ctggctgccc tgaaggggcc gtttaagttt ggtctgtggg ggattgcgat ctacggtgtg
     6781 ctgccttctg agattgcaaa agatgatccg aaaatgatgt caaaaattat gacgtcatta
     6841 ccggccgatg cggtgacgga gactccggca agtactttac cactggacca ggcgacggtt
     6901 cgtgtcagac aacgggttgt ggatgtggtg aaggatgagc ggcagcatat tgcggttgtc
     6961 gcaggtcggc caatgagtgt ccctgtggtg gatgcgaaac cgacaaaacg tccgggggta
     7021 ttcagtgtgt cgattccggg tctcccgtct ctgcaggtga gcgtaccgaa aggtgttccg
     7081 acagcgaaag ccccgccaaa aggcattgtt gctgaaaaag gtgattcacg tccggctggt
     7141 tttacagccg gtggtaactc ccgtgaggcc gttattcgtt tcccgaaaga gaccggacag
     7201 aagccggttt atgtgtcggt gacagatgtt cttaccccgg cacaggtaaa acagcgtcag
     7261 gaggaagaaa agcgtcgcca gcaggcatgg gacgccgctc atccggaaga ggggctgaaa
     7321 agagactatg ataaagcgaa agctgagctg gatgccgaag ataaaaatat tgcgacctta
     7381 aacagccgca ttgcatcgac agagaaggcg ctccccggtg caagggctgc tgttcaggaa
     7441 gccgataaaa aggtaaaaga ggcagaggca aataaagatg attttgtgac ttataacccg
     7501 cctcatgaat atggctccgg gtggcaggat caggttcgct atcttgataa ggatattcag
     7561 aatcagaatg agaaattaaa agcggctcag gcatctttaa acgcaatgaa tgaatcctta
     7621 tccagagata aggctgcact taccggggcg atggagagcc ggaaacaaaa ggagaaaaaa
     7681 gcgaaggatg cagaaaataa gttaaatgag gaaaagaaca agcctcgcaa ggggactaaa
     7741 gactacggcc atgattatca tccagtacct aaaacagaag acataaaggg acttggagag
     7801 ttaaagcgag gtgatcctaa aacacctaaa caaggtggtg gtggtaagcg ggcccgatgg
     7861 tatggcgata aaaaacgtaa aatttatgaa tgggattccc agcacggtga gcttgaaggg
     7921 tatcgtgcca gtgatggcga acacctaggc gcatttgatc ccaaaacggg taagcaggtt
     7981 aaagggccag atccaaaacg gaacattaaa aaatatcttt gagaggtaaa tatggggctt
     8041 aaattacgtt tgacatggtt tgataagaaa actgaagagt ttaaagggga agagtattct
     8101 aaagactttg gtgacgatgg ttctgtcatt gaaagcttgg gaatgccttt aaaggataat
     8161 attaataatg gtgagtttga tttgaagaaa gaatgggttc ctttactaca gccatttttt
     8221 aaaaatcaaa taaaacctga tgtggataat tgttatatag cttttgatta tcgggacggt
     8281 aactggtaac aaaaaattat caaggatgat ttgaaatact gacttccggt gttcagggat
     8341 gaatgccgga gtctttaaat acagggaaag tatatgaaaa aggtaaagac tatttttctt
     8401 tttatattga ttgttgctgg ctttcttctg gccgcatgtc aggcaaatta tatccgggat
     8461 gttcagggcg gaacggtggc accatcgtcc tcctctgaac tgacggggat cgcggttcag
     8521 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc ttttgccctg
     8581 taaacgaaaa aaccacctgg ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg
     8641 cttaaccgtg gtaactggct ttcgcagagc acagcaacca aatctgtcct tccagtgtag
     8701 ccggactttg gcgcacactt caagagcaac cgcgtgttta gctaaacaaa tcctctgcga
     8761 actcccagtt accaatggct gctgccagtg gcgttttgcg tgctttcccg ggttggactc
     8821 aagtgaacag ttaccggata aggcgcagca gtcgggctga acggggagtt cttgcttaca
     8881 gcccagcttg gagcgaacga cctacaccga gccgagatac cagtgtgtga gctatgagaa
     8941 agcgccacac ttcccgcaag ggagaacggc ggacaggtat ccggtaaacg gcagggtcgg
     9001 aacaggagag cgcaagaggg agcgacctac cggaaacggt ggggatcttt aagtcctgtc
     9061 gggtttcgcc cgtactgtca gattcatggt tgagcctcac ggctcccaca gatgcaccga
     9121 aaaagcgtct gtttatgtga actctggcag gagggcggag cctatggaaa aacgccaccg
     9181 gcgcggccct gccgttttgc ctcacatgtt agtcccctgc ttatccacgg aatctgtggg
     9241 taactttgta tgtgtccgca gcgcccgccg cagtctcacg cccggagcgt agcgaccgag
     9301 tgagcgagga ggcgcaattt ccccggcacc tgcaagacgg tcaccggacg cgctgatgtg
     9361 ggatattgtc ccctgattaa gtctttctat tccctgacga tcaattatca ggataggtga
     9421 accatgtcgg tttttcgctg aacattttac gttaacctat ctctgttgct tttttctcct
     9481 tgcggagaaa tgcaagtcat ccccgctggg gcgtggctga cttcagggga aaagaccttg
     9541 cttacgctgg ctggacgcca ccgacgcacg gtcttttccc ctctttctgg ctctgtccgg
     9601 ctggacgccg gccacgccag aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc
     9661 acaaaaatcc acggtgctgg acgcacctga cgaagcagca aagctgctcc tgattttgcg
     9721 ggtggtcagc cgctcgctat ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc
     9781 actggggtgc cgccttttcc tctcacacgc tggggcgtgc tcgttgttcc tccggggctg
     9841 cgccttttcc gcatggggcg gaaagaggct ggcctccgcg gtgaaagctg aacgcaatca
     9901 ccacggacgt gaccgccgcc gccatgcctt ggggcatgtc cgcggcttgt gcagcggggg
     9961 ctgcgcatca cgccggtaaa cccgtcgtct gaaggagaga gattgcatgt ttaatacgga
    10021 ttcgcttgcc tggcagtggc atatcccgga gctggcccgg cagatttacc cgtgggtcag
    10081 ctggctgtac cgggagtatt acagtctggc cggttttttt ctggttatcg gcctgttttt
    10141 cggtctggct gaactctggg ccggccgaat gcgttttgtg gggctgagcc ataaggcgcg
    10201 gcgggagtat gccgatcgct ataagcgcga taataaccgg actgtgccat tttcggtgct
    10261 ggcctcgctg gattcacccg ggcggatggt cagggcacgg cgacatgtct ggaactacgc
    10321 gtactggctg accggcgctg ccggtctggc ccttctgggg gggctggttg tcgccgtgtt
    10381 tatcgagagc tggggagaaa ccgtcctgcg cccggacctc tatgcgaacg cagattatta
    10441 cctgctgcag ccggctaacc tgttcgggat agcctggtgt gccggagggc ttgtcgccgg
    10501 cgtccttctg gcgcggctgc tggcgttcct gctgcttgat ggtcattttg cggccgctga
    10561 cgaggcggtg aacgcgaaac tgaatcagga gagccggcgc agcagccagc ggaccggaga
    10621 aatgacagac gtccgccacc tgcatttcgg ggagccggta ccggttaacg cgctggccga
    10681 tttttccacg gagcaggccc ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg
    10741 ccagccggtg ctggtccctc gtgatacctg gcgcaagacc aatattcaga tcctcggcct
    10801 gcccgggagc ggcaagagcg cgatggccac caacgcgctc attcgctgcg tacgcgattt
    10861 cggggacgct gtggtgtact tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg
    10921 ggcgcactgc ccggacttca ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa
    10981 tctgttccgc gatctcgacc agtacgccct gaagaatctg ctggttgccg ggtttaacct
    11041 gagtgagacc ggcgacgtgg cggaccacta ccgcatcagc gagcagaaag cggcgaagct
    11101 gattgcggag cagttcccgc agggggcaaa cattcagcag gtgctggcgg cggcgtacgc
    11161 gttgccggag gccctgaaaa aggacgtgaa ggggctgatc accaagctgg agaacgtggc
    11221 cgacctgagc gtcctgcaga cggacagcgg tatcgatgtg gcggggataa tcaacggcgg
    11281 cggctgcctg tacgtcatcg gctcgatgga cgatgaggcg gtgatccggg tgcagaagat
    11341 gctgtttgcc cgctgcgcgc aaatcatcat tgcgcgggat gagttccgga ggtggccgca
    11401 cgcgagcatc atgctcgatg agattaaata tctgctgtcg aagtacgtgc tgaacgcgct
    11461 ggggacgctg cgcagccggg actgcaacct gctgctcgcg caccagtcgc tgggcgactt
    11521 cggtcagtgc gggcaggatc tgcccgctga ttttgtgaaa accacggtac tggataacac
    11581 gccgatccgc tggttctatc gcgcggccag ccaggagtcc gcgcagtggg cggcgggcca
    11641 gaccggggag atccgggtgg acgtggagcg ccgccgggcc agccgggagg cggggaacgt
    11701 ggagcacatc agcggcgaca gcttcatcca gaaggaggcg cggccgctgt ttgacgtgaa
    11761 caccctgcaa cacctgcctg acgggttcgc ggtgatgacc gggctgggcg tggcgcggct
    11821 ggcgttcagc agcccgctgc gggttgagcg ccgggaaatt tcgctgaaaa ccttcccggt
    11881 gctggcgaaa acggacccgc tggcggagta cctgcctccg gccgacggcg gcggaccaaa
    11941 agcggatgat gattttgcgg ggctttactg
//
