LOCUS       NZ_CP141631             5596 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KPN6327 plasmid unnamed5, complete
            sequence.
ACCESSION   NZ_CP141631
VERSION     NZ_CP141631.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36780008
            Assembly: GCF_035593365.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Chu,W. and Liu,Z.
  TITLE     Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Chu,W. and Liu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) Department of Clinical Laboratory, the
            Second Affiliated Hospital of Anhui Medical University, FuRong
            Street, Hefei, Anhui 230601, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 31-DEC-2022
            Assembly Method                   :: Fyle v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 11:41:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,970
            CDSs (total)                      :: 5,847
            Genes (coding)                    :: 5,681
            CDSs (with protein)               :: 5,681
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 53 of 166
            Pseudo Genes (incomplete)         :: 116 of 166
            Pseudo Genes (internal stop)      :: 19 of 166
            Pseudo Genes (multiple problems)  :: 20 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141631.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN6327"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="unnamed5"
                     /geo_loc_name="China:Anhui"
                     /collection_date="2021-05-20"
     gene            join(5389..5596,1..17)
                     /locus_tag="Q7W75_RS29800"
                     /old_locus_tag="Q7W75_29720"
     CDS             join(5389..5596,1..17)
                     /locus_tag="Q7W75_RS29800"
                     /old_locus_tag="Q7W75_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1..267
                     /locus_tag="Q7W75_RS29805"
                     /old_locus_tag="Q7W75_29725"
     CDS             1..267
                     /locus_tag="Q7W75_RS29805"
                     /old_locus_tag="Q7W75_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(337..687)
                     /locus_tag="Q7W75_RS29810"
                     /old_locus_tag="Q7W75_29730"
     CDS             complement(337..687)
                     /locus_tag="Q7W75_RS29810"
                     /old_locus_tag="Q7W75_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(753..1298)
                     /locus_tag="Q7W75_RS29815"
                     /old_locus_tag="Q7W75_29735"
     CDS             complement(753..1298)
                     /locus_tag="Q7W75_RS29815"
                     /old_locus_tag="Q7W75_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(1324..1650)
                     /locus_tag="Q7W75_RS29820"
                     /old_locus_tag="Q7W75_29740"
     CDS             complement(1324..1650)
                     /locus_tag="Q7W75_RS29820"
                     /old_locus_tag="Q7W75_29740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(2008..2166)
                     /locus_tag="Q7W75_RS29825"
     CDS             complement(2008..2166)
                     /locus_tag="Q7W75_RS29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2806..2982
                     /locus_tag="Q7W75_RS29830"
                     /old_locus_tag="Q7W75_29745"
     CDS             2806..2982
                     /locus_tag="Q7W75_RS29830"
                     /old_locus_tag="Q7W75_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2972..3316
                     /locus_tag="Q7W75_RS29835"
                     /old_locus_tag="Q7W75_29750"
     CDS             2972..3316
                     /locus_tag="Q7W75_RS29835"
                     /old_locus_tag="Q7W75_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3525..>3959
                     /locus_tag="Q7W75_RS29840"
                     /old_locus_tag="Q7W75_29755"
                     /pseudo=""
     CDS             3525..>3959
                     /locus_tag="Q7W75_RS29840"
                     /old_locus_tag="Q7W75_29755"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3994..4503
                     /locus_tag="Q7W75_RS29845"
                     /old_locus_tag="Q7W75_29760"
     CDS             3994..4503
                     /locus_tag="Q7W75_RS29845"
                     /old_locus_tag="Q7W75_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            4510..4722
                     /locus_tag="Q7W75_RS29850"
                     /old_locus_tag="Q7W75_29765"
     CDS             4510..4722
                     /locus_tag="Q7W75_RS29850"
                     /old_locus_tag="Q7W75_29765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2641..2720
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(2058..2455)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3307..4838
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttggtctgga cgattaacta ttccgatcgg gcgctcaaat cgttacgcaa gatggacaaa
       61 cagaacgcac gacggattgt ggattttatg agtttacgca ttgcagttgc tgccgatcct
      121 cgccagtcag ggaagccgct caaaggtgag ctgggcgagt tctggcgcta tcgggtggga
      181 gattatcgcg ttctgtgtga gatccgagat gacgagcttg ttatccttgc cgccacgatt
      241 ggacatcgcc gcgaagttta cgactgaagc ccgctatgcg ggcttttttg atgccgacaa
      301 caggtattat ggtaaatact cgactaacta cgtgttctac tgtttgtcgg ttttagttaa
      361 ccagcaggtt gtcttaacga taatgtagcc gttattttta actaataagg tcatattgtt
      421 aacgtcaggc ataataaaag acattatggc ctcagagtta tcatcagaag cagctctctg
      481 cattactact tcgattccct tttctttgaa tctataagta tccagttgcc tattaaactt
      541 agaaagacct acaaccatac ttagtttgtt atctttaaca gtaagagatg cttcctgagt
      601 accattacat cggtagtaag cggtgtaacc gtcagcaaaa gatcgaccgg ggaatataaa
      661 tgctaggaac gcaattaacc aatacatagt tgagaatctt ggtattacac atttcactat
      721 cctctctttc atttttcgtt caccatactt tgttatctga tttttaattt ttctatttta
      781 taaaccaagt ctctaccaaa aaggcgaaaa ttatcctcaa ataataataa ttgtttaaga
      841 acatccatga aagcagctgc ttcaccctca gttatgggag ctactctata taacatataa
      901 cctgagttaa gggtttcttt tatactttct tcaatgctca ttataaatat tagtggatta
      961 tcatttttct tcccccatgg gtttctaatt aatccttcga tgtactcagg tcctttgcgt
     1021 cctattgcct gcgcaatatt ttttatttca tctgcgtgag ggctttgttg tacagctttg
     1081 tttatgcctg cttgagtgat tttcagagac tctacaccgt gtatgttgta tgagataaaa
     1141 tgtaataaat taagatgcaa tgcaattaat ccatttttgt ttacatctga gtatttggat
     1201 aaaaaacgta atgaagtgtt aactctccct tgaagcctaa caattgggag tgctgccttg
     1261 tatatttcct tctccgttaa ataccgttta aaaaacataa ttctccgcct ccgtatagcg
     1321 tgcctatttt atttcgtcgt aaaacttagc tctgaaatat tcctttagaa ccatcatgtc
     1381 attaataatt gaatctatct catccatttt ttcttggtat tcttttaatg gtgaagggag
     1441 cggtaactca atatttgaaa agtgattggc gagataggga tcaaggtttc tcatccattc
     1501 aggcttttcg ccaatgcgtt cttcaactga aagtgaacct gataatctct ggttaataag
     1561 atcacttaga ctcattttgg ttttattcga aagccgttgt aaggattgct ttaatcctat
     1621 atctagcatg atatttgttc tttctctcat taccccccct tgctattgcg ttttttgttg
     1681 cgcacaaagc taccattgcg cattaagaaa cgcaataaaa aaggagggag ggagtggttt
     1741 gtacatgatg tgcagtgcgc tgagggcatc agtggcgggt gtttttccgt gggatggatg
     1801 gagctccggg gataacagaa ctgacgatca tttaaaggat cttcttgaga tccttttttt
     1861 ctgcgcgtaa tctcttgcac tgtaaacgaa aaaaccgcct ggggaggcgg tttgatcgaa
     1921 ggttaagtca gttggggaac tgcttaaccg ggtaactggg tatacgagac cgcagatacc
     1981 aaaactgttc tttcagtgta gccgcagtta ggcacccact tcaagacctc tcaatatctc
     2041 tctcgtatat atccagttac caatggctgc tgccagtggc gtttttgcgt gtccgtccgg
     2101 gttggactca agatgatagt taccggaaag ggcgcagcag tcgggctgaa cggggggttc
     2161 gtgcatacag cccagcttgg agcgaacgac ctaaaccgag ccgagatacc agcagcgtga
     2221 gctatgagaa agcgccacgc ttcccgaaag ggaaaaaggc ggaacaggta tccggtaaac
     2281 ggcagggtcg gaacaggaga gcgcacgagg gagccgccgg agagaaatca ccggcatctt
     2341 taagtcctgt cgggtttcgt cctcctctga cttgagcgtc catttctgtg atgctcgtca
     2401 ggggaggcgg agcctatgga aaaacggcaa cgccgcatcc ttttctccgg ccctttctga
     2461 tttgagcgcc tttgctttat cccgttatcc gtgagtcggt cacgcccgcc gcagccgaac
     2521 ggcaggagcg cagctaccga gtgagcgagg aggcgtcatt tcatctgtcc gtgcatggta
     2581 ttgcggacgc tcctgagcca catatcacac ctgccacatg aggcactttc atgaatcgtg
     2641 ctctcatgcc aacatagtaa gccagtatac acagcgtaaa cgctgtgact ggttcagggc
     2701 tgcgccccga aacccgctaa caccactgac gcgctggcgc ttgtccaacg ccggagcgac
     2761 ccggaaaact tttccgtgcc gtccggcgtt tgcgaaggag ggataatgca gaagccgaag
     2821 aagaagccgc tcgtcgtaaa aaaaagatcc tggcatccgg cagtgatggc aaatctgatg
     2881 cgtcaggcac aggaggaaga ttaccgccgg gagcagatgg cagagcttga gcgttcagcc
     2941 cggtcacatt ttgcggatat cggaggagag gatggcggtt aagcgtaccc gttttttagg
     3001 tatccgggtg accgatgggg agtaccagca gctgctggag cgctgcaatg gaaggcaact
     3061 ggcggtgtgg atgcgggaaa cctgtctcga tacgcgcccg gcgcggtcgt tgcggctgcc
     3121 gtccatcgat ccggttttgc tgcgccagct tgccgggatg gggaacaacc tcaaccagat
     3181 cgcccggaaa attaacggcg gccagtggtc aggcgctgac cgggttcagg tggtggccgc
     3241 gctgatggcc attgatgccg aactcgagcg gctgcggcat gccgtgctgg aaaagggggc
     3301 agacgatgat cgttaagttt catccgcgag ggcgaggcgg cggtgccggg ccggtggatt
     3361 atctgctggg gaaagaccgc cagcgcgacg gggccatcgt cctgcaggga aagccggagg
     3421 aggtccggga gcttatcgac gcctcgccct acgccaaaaa gtacacctcc ggcgtcctgt
     3481 cttttgctga acaggattta ccgcccggcc agcgtgaaaa gctgatggcg agcttcgagc
     3541 gggttctgat gcccggactc gataaagacc agtacagcgt gctgtgggtt gaacaccggg
     3601 acaaggggcg gctggaactg aattttctgg taccgaacac ggaactgctg accggcaggc
     3661 gtctccagcc gtactacgac cgggcagacc gtccgcgcat cgatgcgtgg cagaccgtgg
     3721 tgaacgggcg actggggctg cacgacccga acgcaccgga gaaccggcgc gcgctggtga
     3781 cgccatcttc gttacccaaa acgaagcagg aagccgcaga ggctattacg cggggcttac
     3841 tggccctcgc ctcgtcaggg gagcttaaaa cgcgtcagga cgtcactgag gcgctggaaa
     3901 gcgcaggttt tgaggtcgtg cgcaccacaa agagcagcat cagcattgcc gacccggacg
     3961 gagggcgaaa catccgactt aagggagcca tctatgaaca gtcttttaac gctggcgaag
     4021 gacttagagc agaaatcgaa agcgcagcag cagagtaccg gcgaaatgct gaaagccgca
     4081 ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgggaa gagaatcagc
     4141 gccgccatcc gcgcccacga gcagggtatg agcgcggcca tgcagtcgaa ccgcctgagc
     4201 gtgatgcgta tggtcgggcg gacatggctg accatcacga tggtgtcagg actgctgttc
     4261 gccagcctga gcggggtgtt gtggtatcag gggagcttga tagcgtcaaa tctggccgaa
     4321 atcgaccggc agaacgcagc gctgtcgaag ctgaacgcga agacctgggg cgtgacgtac
     4381 ctggaagaca gcaacggacg ttttctggtg ctgccgaagg ggacggccgt agacaggacg
     4441 cagagctgga cggtcgggaa cgggcggtcg aagcagaacg cgctgagact ggtgaaggag
     4501 taagtgccga tgaccgagct ggaaaaacag ttgctgagcg cattagagca gctacagcag
     4561 gactactcga aaaggctgga cgagtgggag agcgccttcg gggaatggcg gaaaatgtca
     4621 gggcttatgc aacgggagaa cgcggcgctg agcgagcgcg tgacgcgctt gagtcagcag
     4681 gtggaacgct tgagcgggca actgcagcgt ttgagccggt agtgcagcgt catgagatgg
     4741 ccgtggccgc agaacgggcg cacgagcaac ggcagcacga aaaggaactg acggaagcgc
     4801 gttcccggca gcgaagctat gacggtccgt cgctgggctg aaatgcagga aaacagggtt
     4861 ctcatcgggc atttacgctg aaaccggatg tcattcacct ggctgcatgg ctatgcagcc
     4921 aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg tggtttgttg ccggttttac
     4981 ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg tgcgggcaac gggtgttatg
     5041 tttaatcagc gtgaacggca gatacaaaaa agccccggag ggcttttaac tactgactta
     5101 tttttttgaa gatttgctgg gtttcaattt gtcagtattt cccggcgttt ttttattgtc
     5161 acgctggcgt ttatcaggtt cccctgttga atcggcaatt cgtttcggac ccggcttaag
     5221 acccataatc gttatccttt caatagttaa gaggaaagcc ctcaagctag atatattgac
     5281 cgcgttacgg attatcaaga ggggctaaca agattatttt ttgttctttt aggttgattg
     5341 tggtactgtt gatatacatg tatagcaaat ggtgttcggg aggtttatat gcttgctatc
     5401 cggttatctg atgagattga gtcccgtctg gactcgctgg cgaagcaaac cggcagaaca
     5461 aagacgtttt atgcgcggga agcaatactg gcgcatctgg aagacctgga ggattattat
     5521 ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg aagcagtgca ttcgtctgaa
     5581 gacgtgagga agtcac
//
