LOCUS       NZ_CP141630            12918 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KPN6327 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP141630
VERSION     NZ_CP141630.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36780008
            Assembly: GCF_035593365.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12918)
  AUTHORS   Chu,W. and Liu,Z.
  TITLE     Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12918)
  AUTHORS   Chu,W. and Liu,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2023) Department of Clinical Laboratory, the
            Second Affiliated Hospital of Anhui Medical University, FuRong
            Street, Hefei, Anhui 230601, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 31-DEC-2022
            Assembly Method                   :: Fyle v. V2.5
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 400.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 11:41:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,970
            CDSs (total)                      :: 5,847
            Genes (coding)                    :: 5,681
            CDSs (with protein)               :: 5,681
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 166
            CDSs (without protein)            :: 166
            Pseudo Genes (ambiguous residues) :: 0 of 166
            Pseudo Genes (frameshifted)       :: 53 of 166
            Pseudo Genes (incomplete)         :: 116 of 166
            Pseudo Genes (internal stop)      :: 19 of 166
            Pseudo Genes (multiple problems)  :: 20 of 166
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141630.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12918
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN6327"
                     /isolation_source="blood"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China:Anhui"
                     /collection_date="2021-05-20"
     gene            join(12653..12918,1..13)
                     /locus_tag="Q7W75_RS29720"
                     /old_locus_tag="Q7W75_29640"
     CDS             join(12653..12918,1..13)
                     /locus_tag="Q7W75_RS29720"
                     /old_locus_tag="Q7W75_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1..315
                     /locus_tag="Q7W75_RS29725"
                     /old_locus_tag="Q7W75_29645"
     CDS             1..315
                     /locus_tag="Q7W75_RS29725"
                     /old_locus_tag="Q7W75_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            479..907
                     /locus_tag="Q7W75_RS29730"
                     /old_locus_tag="Q7W75_29650"
     CDS             479..907
                     /locus_tag="Q7W75_RS29730"
                     /old_locus_tag="Q7W75_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            933..1112
                     /locus_tag="Q7W75_RS29735"
                     /old_locus_tag="Q7W75_29655"
     CDS             933..1112
                     /locus_tag="Q7W75_RS29735"
                     /old_locus_tag="Q7W75_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1139..1669)
                     /locus_tag="Q7W75_RS29740"
                     /old_locus_tag="Q7W75_29660"
     CDS             complement(1139..1669)
                     /locus_tag="Q7W75_RS29740"
                     /old_locus_tag="Q7W75_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1676..2407)
                     /gene="mobC"
                     /locus_tag="Q7W75_RS29745"
                     /old_locus_tag="Q7W75_29665"
     CDS             complement(1676..2407)
                     /gene="mobC"
                     /locus_tag="Q7W75_RS29745"
                     /old_locus_tag="Q7W75_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2407..4290)
                     /locus_tag="Q7W75_RS29750"
                     /old_locus_tag="Q7W75_29670"
     CDS             complement(2407..4290)
                     /locus_tag="Q7W75_RS29750"
                     /old_locus_tag="Q7W75_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_305734531.1"
                     /translation="MLSYYSLAGFFLVIGLFFGLAELWAGRMRFVGLSHKARREYADRY
                     KRDNNRTVPFSVLASLDSPGRMVRARRHVWNYAYWLTGAAGLALLGGLVVAVFIESWGE
                     TVLRPDLYANADYYLLQPANLFGIAWCAGGLVAGVLLARLLAFLLLDGHFAAADEAVNA
                     KLNQESRRSSQRTGEMTDVRHLHFGEPVPVNALADFSTEQARKQQAVFLGKDEQGQPVL
                     VPRDTWRKTNIQILGLPGSGKSAMATNALIRCVRDFGDAVVYFDPKGDAWAPHVFRAHC
                     PDFTLLDLRPGKPAQLNLFRDLDQYALKNLLVAGFNLSETGDVADHYRISEQKAAKLIA
                     EQFPQGANIQQVLAAAYALPEALKKDVKGLITKLENVADLSVLQTDSGIDVAGIINGGG
                     CLYVIGSMDDEAVIRVQKMLFARCAQIIIARDEFRRWPHASIMLDEIKYLLSKYVLNAL
                     GTLRSRDCNLLLAHQSLGDFGQCGQDLPADFVKTTVLDNTPIRWFYRAASQESAQWAAG
                     QTGEIRVDVERRRASREAGNVEHISGDSFIQKEARPLFDVNTLQHLPDGFAVMTGLGVA
                     RLAFSSPLRVERREISLKTFPVLAKTDPLAEYLPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4314..5705)
                     /locus_tag="Q7W75_RS29755"
                     /old_locus_tag="Q7W75_29675"
     CDS             complement(4314..5705)
                     /locus_tag="Q7W75_RS29755"
                     /old_locus_tag="Q7W75_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493761.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4-like element ISKpn19 family transposase"
                     /protein_id="WP_001493761.1"
                     /translation="MQLTLQIVITDESGSSRTEELMTIQKSGETRNDIGLSVSESKLLL
                     NTVQQSVVQLQADEYTQHHIRCPHCLAARRIKGKQKIRYRTLFGVIPVSGLRVYRCRCE
                     ESDTKTVSLLSDWAGDYSHPALKYIETRWASMISYEMTTRLLKDILPVGHSLNASTVRN
                     HLCQVAQRLDAEAEAHSGFLSGCPRDWGNLPRPGKPLVVGIDGGYVRDRDDKKRNFEII
                     AGKSFSVGAPADTRRFGFVQKDDCHPERRLMTHLSAQGMQANQQIFFLSDGADNLRDLQ
                     FGMYPESTHVLDWFHITMRLKVLMQYARGLLVSDPEAGSKVLALLESIKRYLWHGNVVA
                     ALEHIDNCVMYCDDPELSYPSLKSLQKHLDEMYTYIRNNKMMIPNYGEMRRYGEPVSTA
                     FVESTINEVIARRMAKKQQMQWSRKGAHYLLQTRTAVLNNELQDKFVCWYPGFQSDGKG
                     PAMAA"
                     /name="ISKra4-like element ISKpn19 family transposase"
     gene            complement(5742..6314)
                     /locus_tag="Q7W75_RS29760"
                     /old_locus_tag="Q7W75_29680"
     CDS             complement(5742..6314)
                     /locus_tag="Q7W75_RS29760"
                     /old_locus_tag="Q7W75_29680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493762.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001493762.1"
                     /translation="MQYAYIRVSSADQNTARQEEALSKAGFQPDKICVEHASAKDTNRP
                     GLQELLGQLRPGDTLLVHSIDRLCRNMSDMCAVTTRLRDQGVTLIFLKEQLTFSAGTNN
                     PMQELQLHMMSAFSQFERALLKERQADGIAAKKARGEKTGRPGADIKKIQEIDALRSRG
                     VRLRIACDHAGLGVSTYYKLRHQMNNQ"
                     /name="recombinase family protein"
     gene            complement(6451..6996)
                     /locus_tag="Q7W75_RS29765"
                     /old_locus_tag="Q7W75_29685"
     CDS             complement(6451..6996)
                     /locus_tag="Q7W75_RS29765"
                     /old_locus_tag="Q7W75_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="WP_001493763.1"
                     /translation="MVWRRLRIAADTSLAALHFIFQIVQGWGDDHLHQFHIYGKDYGIS
                     YEGGIGFVDNPFRVVIDDFAFDAGDRFTYEYNFFEHWLHDIRVEAIYENSTLKAPFCIS
                     GHGMPGATAADEFDKTLAFLEAIVNADDETTVGEIRPFADDLDAVRFNRHKINRQLSRL
                     DLASPVLEPEVIWLGRRR"
                     /name="plasmid pRiA4b ORF-3 family protein"
     gene            complement(8713..8862)
                     /locus_tag="Q7W75_RS29770"
                     /old_locus_tag="Q7W75_29690"
     CDS             complement(8713..8862)
                     /locus_tag="Q7W75_RS29770"
                     /old_locus_tag="Q7W75_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8947..9204)
                     /locus_tag="Q7W75_RS29775"
                     /old_locus_tag="Q7W75_29695"
     CDS             complement(8947..9204)
                     /locus_tag="Q7W75_RS29775"
                     /old_locus_tag="Q7W75_29695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(9214..10899)
                     /locus_tag="Q7W75_RS29780"
                     /old_locus_tag="Q7W75_29700"
     CDS             complement(9214..10899)
                     /locus_tag="Q7W75_RS29780"
                     /old_locus_tag="Q7W75_29700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            11226..11471
                     /locus_tag="Q7W75_RS29785"
                     /old_locus_tag="Q7W75_29705"
     CDS             11226..11471
                     /locus_tag="Q7W75_RS29785"
                     /old_locus_tag="Q7W75_29705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            11745..12116
                     /gene="tolA"
                     /locus_tag="Q7W75_RS29790"
                     /old_locus_tag="Q7W75_29710"
     CDS             11745..12116
                     /gene="tolA"
                     /locus_tag="Q7W75_RS29790"
                     /old_locus_tag="Q7W75_29710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            12113..12478
                     /locus_tag="Q7W75_RS29795"
                     /old_locus_tag="Q7W75_29715"
     CDS             12113..12478
                     /locus_tag="Q7W75_RS29795"
                     /old_locus_tag="Q7W75_29715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     BGC             4214..12918
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          8032..8610
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1680..2407)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 atgccacagg taacgatttc cgcgctggca cagcgcgatt tacaacgcct tcaggacttt
       61 ctcaaaacca aaaatcggct ggcagccaga aaaggaggtg aggtgatcgt ccgggctatc
      121 cagcaactga aaacattgcc agacattggc cgcccggtgc cgtttctgcc gctggaatat
      181 aaggaactgg tgatcgggtt tggcgacagt ggctatgtga tgctctaccg ccacgaccgg
      241 gaaatggacc agattgtgat cgtcaccgtc aggcatcaga aagaatccgg gtatccgggg
      301 gcagacagcc tctaataacc gatgacgggg gcgcaattca ttgcgtggcc gtcgccggtt
      361 tgacaagcgc agcgcgtcag aaaaccacat cctgaacgga aaaacgggta aaattcagcg
      421 atacatttac cgtgagaaat tacacacctt tcacacccga taaaatcagg ggtatacagt
      481 ggcgaaaaca ttgctcaacc tttcacaggc agcacaagcc gcgggcatta ccagaagaac
      541 gctatacaat catgtaaaac agggaaaggt taccgtgtca cgtgacggga aaaacaaccc
      601 tgtcgtggat gtgtcagaac tgatccgtgt atacggaaat gtgaatatcc ctgagaaaca
      661 aattcccggc atttcacacc gtgaaaatac acaaaaaaat ttcccacagg aacagcttct
      721 ggcaatgcaa aaggaactgg ccgatctccg gcaggctgtc accctgatgc tggaagataa
      781 aacatcccgg gaagaagaac gccgccagca cgatgatgag cgccggaaac tccaggccga
      841 agtggacaga ttgacaacag aactgacaca gaagaaaaaa cgcttctggt ccggctggtt
      901 tagctgagaa cttaccgaag ggaatggaga acatgacgaa gcaggaaaaa actgccctca
      961 atatggctaa attcattaag gatcagtcgc tgctgttgct ggaaaagctg aacgagctgg
     1021 acatggatac cccggccgac atgtgcgaac ggcttcacga ggatgcggaa acactctggc
     1081 aggtgatgcg ggataagctc ggggaagaat aacagcaccg gccatgccgg tgctggcgtt
     1141 attttttgcc gaacggccac caccggcggg cgggtttgtc ctccggcagt tcaggcaccg
     1201 gcacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc ttctcgttca
     1261 ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat cgcgttaatc tgctccggtg
     1321 agcacttccc ggcatcaacc acctgcacac cgttctcgat ggcgaaccgc tccacctttc
     1381 gatagaggta atcacacagc ttcattcctg tgggtttgtc ccgttcccat tgcggatttt
     1441 caccggtgaa aaagtacgtg gctttttcgt cgctgttgag ccgctcgtca tacatcccga
     1501 tggatttcat catcgccaga tgctgggccc ggtcgacaaa acagaaaaca tacgccccgg
     1561 tctgccaggc gagcaccacc atgtcttcca gatgctcgct ggttggcata atgcagcgct
     1621 cgccgtcttt gtcttccacg tcctcaataa ataccatgta cagcgccata atccctcact
     1681 ccgttgtcat aaatttaaag cacgcgaaca gcctctcccg gtccagctcc accgttttgc
     1741 gggcctcgcc ggtctgtgga ttcacgattt catccaccgc cagaatcgcc cgctgtaccc
     1801 ggacaagcgt cggccggtca gggcagatgt accagatttc atcgatgccg ttcgcccggc
     1861 ggttgaacag atacccggac accacttcct gataccgcct gcggctcttg acggtgcgct
     1921 ctacctcaaa tgccaccgtt ctgccgtcca gctccgccag cgcgtccggc actttcatcc
     1981 ctttcagggc catacggtgc aggtaccgcc agccggtggc cccccggccc tccatggaga
     2041 ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc cttgacggct
     2101 gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa cgagaacgcc accccgtgcg
     2161 gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg gtatttcgcc cggaccagca
     2221 gccctttcgc caccagactg tccaggaagc ggtaaaccgg ctgtaccgcc gcaatcccca
     2281 gccgtcgcgc aaccagctcc gccgtggaac acagctcttc ccgcagccag cgcagggtca
     2341 gccgtgtttt ctccgcgttc cgcgcctgcc gcttcgcgtg gctgacgttg tactgctcca
     2401 gtgtcatcag taaagccccg caaaatcatc atccgctttt ggtccgccgc cgtcggccgg
     2461 aggcaggtac tccgccagcg ggtccgtttt cgccagcacc gggaaggttt tcagcgaaat
     2521 ttcccggcgc tcaacccgca gcgggctgct gaacgccagc cgcgccacgc ccagcccggt
     2581 catcaccgcg aacccgtcag gcaggtgttg cagggtgttc acgtcaaaca gcggccgcgc
     2641 ctccttctgg atgaagctgt cgccgctgat gtgctccacg ttccccgcct cccggctggc
     2701 ccggcggcgc tccacgtcca cccggatctc cccggtctgg cccgccgccc actgcgcgga
     2761 ctcctggctg gccgcgcgat agaaccagcg gatcggcgtg ttatccagta ccgtggtttt
     2821 cacaaaatca gcgggcagat cctgcccgca ctgaccgaag tcgcccagcg actggtgcgc
     2881 gagcagcagg ttgcagtccc ggctgcgcag cgtccccagc gcgttcagca cgtacttcga
     2941 cagcagatat ttaatctcat cgagcatgat gctcgcgtgc ggccacctcc ggaactcatc
     3001 ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc ttctgcaccc ggatcaccgc
     3061 ctcatcgtcc atcgagccga tgacgtacag gcagccgccg ccgttgatta tccccgccac
     3121 atcgataccg ctgtccgtct gcaggacgct caggtcggcc acgttctcca gcttggtgat
     3181 cagccccttc acgtcctttt tcagggcctc cggcaacgcg tacgccgccg ccagcacctg
     3241 ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc ttcgccgctt tctgctcgct
     3301 gatgcggtag tggtccgcca cgtcgccggt ctcactcagg ttaaacccgg caaccagcag
     3361 attcttcagg gcgtactggt cgagatcgcg gaacagattc agctgcgccg gtttccccgg
     3421 ccgcagatcc agcagggtga agtccgggca gtgcgcccgg aagacgtgcg gcgcccacgc
     3481 gtcccctttg gggtcgaagt acaccacagc gtccccgaaa tcgcgtacgc agcgaatgag
     3541 cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg ccgaggatct gaatattggt
     3601 cttgcgccag gtatcacgag ggaccagcac cggctggccc tgctcatcct tacccagaaa
     3661 caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg gccagcgcgt taaccggtac
     3721 cggctccccg aaatgcaggt ggcggacgtc tgtcatttct ccggtccgct ggctgctgcg
     3781 ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca gcggccgcaa aatgaccatc
     3841 aagcagcagg aacgccagca gccgcgccag aaggacgccg gcgacaagcc ctccggcaca
     3901 ccaggctatc ccgaacaggt tagccggctg cagcaggtaa taatctgcgt tcgcatagag
     3961 gtccgggcgc aggacggttt ctccccagct ctcgataaac acggcgacaa ccagcccccc
     4021 cagaagggcc agaccggcag cgccggtcag ccagtacgcg tagttccaga catgtcgccg
     4081 tgccctgacc atccgcccgg gtgaatccag cgaggccagc accgaaaatg gcacagtccg
     4141 gttattatcg cgcttatagc gatcggcata ctcccgccgc gccttatggc tcagccccac
     4201 aaaacgcatt cggccggccc agagttcagc cagaccgaaa aacaggccga taaccagaaa
     4261 aaaaccggcc agactgtaat aggagagcat aaaaaatatt ggggcagcgt gagctatgct
     4321 gccatcgctg gtcccttccc gtcactttga aaacccggat accagcagac aaatttatcc
     4381 tgcagttcgt tattcaaaac ggcggtccgg gtctgtaaca aataatgagc cccttttctg
     4441 ctccactgca tctgctgctt tttggccatt cgtctggcga tcacttcatt gatcgtggat
     4501 tccacaaacg cggttgatac cggctctccg taccgacgca tttcgccgta gtttgggatc
     4561 atcatcttat tattccggat gtaggtatac atttcatcca gatgtttttg cagggatttc
     4621 aggctaggat agctgagttc agggtcatcg caatacataa cgcaattatc aatatgttcc
     4681 agcgcagcga caacattgcc atgccagaga tatcgtttaa tgctttccag cagtgccaga
     4741 actttactcc ccgcctctgg atctgatacc agcagtcccc gggcatattg catgagcacc
     4801 ttcagcctca tggtgatatg aaaccagtcc agcacatgcg ttgactcagg gtacataccg
     4861 aactggaggt ccctgagatt atccgcgccg tcggacagaa aaaatatctg ctggttggcc
     4921 tgcattccct gcgctgaaag gtgggtcatg agcctgcgtt ccggatgaca gtcatccttc
     4981 tgaacaaaac cgaagcggcg ggtgtcagca ggcgcaccaa cggaaaaaga cttcccggca
     5041 ataatttcaa aattacgctt tttgtcatcc cgatcgcgaa cataaccacc atctataccc
     5101 accacaagcg gttttcctgg cctgggcagg ttcccccagt cgcgggggca gccggaaaga
     5161 aaacctgaat gagcttcagc ctcagcgtca agacgctgcg ccacctggca taaatgattc
     5221 ctcaccgttg aggcattcag gctgtggccc accggcagaa tatctttcag cagacgagtc
     5281 gtcatttcat aggagatcat cgaggcccag cgggtttcaa tatatttcag tgccgggtga
     5341 gaataatcgc cggcccagtc gctgagcaaa ctgactgtct ttgtatcact ctcttcacaa
     5401 cggcaccggt acacccgaag cccggatacc ggaataacgc caaacagcgt ccggtaccgt
     5461 attttctgtt tgcctttgat tctgcgcgca gcaaggcagt gagggcaccg gatatggtgt
     5521 tgagtatatt cgtctgcctg cagctggacc accgactgct ggaccgtatt cagcaacagc
     5581 ttggattctg acaccgataa tccgatgtcg ttccgggtct cccctgattt ctggattgtc
     5641 atcagttcct ccgttcgact ggagcctgat tcatcggtga tgactatctg gagcgtcagt
     5701 tgcatcattg cctcctgctg tggtggatga ctatctcccg gctactggtt gttcatctgg
     5761 tgacgaagct tataatatgt ggaaacgcca aggcccgcat ggtcgcaggc gatcctgagc
     5821 ctgacccctc tgcttctgag tgcgtcaatt tcctggatct ttttgatatc ggcgccgggg
     5881 cgccctgttt tttcaccccg tgcttttttc gcggcgatgc cgtcggcctg cctttccttc
     5941 agtaaggctc gttcaaactg gctgaatgcc gacatcatgt gcagctgcag ttcctgcatc
     6001 ggattgtttg tgccggcgct gaacgtcagc tgctctttca gaaaaataag ggtaacgccc
     6061 tggtcacgaa gccgggtggt gacagcgcac atatccgaca tattccggca aaggcgatcg
     6121 atggaatgaa ccagaagcgt atcaccaggg cgtaattgcc ccaggagttc ctgtaacccc
     6181 gggcggtttg tgtccttcgc gctggcatgc tcaacacaaa ttttatcagg ctgaaaaccc
     6241 gcctttgaca atgcctcttc ctgacgggcc gtattctgat cggccgagct taccctgatg
     6301 taggcatatt gcacgcaaaa cctccggaag gtctttaaac atcatagaga tttaaagaat
     6361 aatccataaa cactgattta gggaggttta tagacatttt ttcggggatc tgagaggcat
     6421 gctgaaaacg tataccttta tagacatcag tcagcgacga cggcccagcc agataacttc
     6481 aggctccagt accggagatg caaggtcgag cctgctcagt tgccggttga ttttgtggcg
     6541 gttgaacctg acagcatcca ggtcgtcagc aaaagggcgg atctcgccga ccgttgtttc
     6601 atcatccgca ttaacgatag cttcaagaaa cgccagggtt ttatcgaact catccgcagc
     6661 tgtggctccg ggcataccat ggccgcttat acaaaacggc gctttcagcg tagagttttc
     6721 ataaatggcc tcaacacgaa tgtcatgaag ccagtgctca aagaaattgt attcataggt
     6781 aaagcgatcg ccagcatcaa aagcaaaatc atcaatcacg acccggaacg gattatcgac
     6841 aaaaccgatg cctccttcgt aggagatacc gtaatctttg ccataaatgt gaaattgatg
     6901 gagatggtcg tccccccagc cctgcactat ctggaaaatg aagtgaagcg cggccagtga
     6961 cgtgtcggca gcaattctca gccgacgcca gaccatcgga ctgactccat gaactgctat
     7021 tttgatgacg tagatcttca tagtcagcag gtattgttat gaaatgatgg tggttatttt
     7081 aacccctgta gatggcatca ttcgatggct gccccgaggt ttttcatgct ctcctaatac
     7141 tcccggtaca gccagctgac ccacgggtaa atctgccggg ccagctccgg gatatgccac
     7201 tgccaggcaa gcgaatccgt attaaacatg caatctctct ccttcagacg acgggtttac
     7261 cggcgtgatg cgcagccccc gctgcacaag ccgcggacat gccccaaggc atggcggcgg
     7321 cggtcacgtc cgtggtgatt gcgttcagct ttcaccgcgg aggccagcct ctttccgccc
     7381 catgcggaaa aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa
     7441 aggcggcacc ccagtgcggt cttttccggt gttccggccc ccgtcagagt tcgccatagc
     7501 gagcggctga ccacccgcaa aatcaggagc agctttgctg cttcgtcagg tgcgtccagc
     7561 accgtggatt tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg aatttctggc
     7621 gtggccggcg tccagccgga cagagccaga aagaggggaa aagaccgtgc gtcggtggcg
     7681 tccagccagc gtaagcaagg tcttttcccc tgaagtcagc cacgccccag cggggatgac
     7741 ttgcatttct ccgcaaggag aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg
     7801 aaaaaccgac atggttcacc tatcctgata attgatcgtc agggaataga aagacttaat
     7861 caggggacaa tatcccacat cagcgcgtcc ggtgaccgtc ttgcaggtgc cggggaaatt
     7921 gcgcctcctc gctcactcgg tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg
     7981 acacatacaa agttacccac agattccgtg gataagcagg ggactaacat gtgaggcaaa
     8041 acggcagggc cgcgccggtg gcgtttttcc ataggctccg ccctcctgcc agagttcaca
     8101 taaacagacg ctttttcggt gcatctgtgg gagccgtgag gctcaaccat gaatctgaca
     8161 gtacgggcga aacccgacag gacttaaaga tccccaccgt ttccggtagg tcgctccctc
     8221 ttgcgctctc ctgttccgac cctgccgttt accggatacc tgtccgccgt tctcccttgc
     8281 gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt gtaggtcgtt
     8341 cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg cgccttatcc
     8401 ggtaactgtt cacttgagtc caacccggga aagcacgcaa aacgccactg gcagcagcca
     8461 ttggtaactg ggagttcgca gaggatttgt ttagctaaac acgcggttgc tcttgaagtg
     8521 tgcgccaaag tccggctaca ctggaaggac agatttggtt gctgtgctct gcgaaagcca
     8581 gttaccacgg ttaagcagtt ccccaactga cttaaccttc gatcaaacca cctccccagg
     8641 tggttttttc gtttacaggg caaaagatta cgcgcagaaa aaaaggatct caagaagatc
     8701 ctttgatctt ttctactgaa ccgcgatccc cgtcagttca gaggaggacg atggtgccac
     8761 cgttccgccc tgaacatccc ggatataatt tgcctgacat gcggccagaa gaaagccagc
     8821 aacaatcaat ataaaaagaa aaatagtctt tacctttttc atatactttc cctgtattta
     8881 aagactccgg cattcatccc tgaacaccgg aagtcagtat ttcaaatcat ccttgataat
     8941 tttttgttac cagttaccgt cccgataatc aaaagctata taacaattat ccacatcagg
     9001 ttttatttga tttttaaaaa atggctgtag taaaggaacc cattctttct tcaaatcaaa
     9061 ctcaccatta ttaatattat cctttaaagg cattcccaag ctttcaatga cagaaccatc
     9121 gtcaccaaag tctttagaat actcttcccc tttaaactct tcagttttct tatcaaacca
     9181 tgtcaaacgt aatttaagcc ccatatttac ctctcaaaga tattttttaa tgttccgttt
     9241 tggatctggc cctttaacct gcttacccgt tttgggatca aatgcgccta ggtgttcgcc
     9301 atcactggca cgataccctt caagctcacc gtgctgggaa tcccattcat aaattttacg
     9361 ttttttatcg ccataccatc gggcccgctt accaccacca ccttgtttag gtgttttagg
     9421 atcacctcgc tttaactctc caagtccctt tatgtcttct gttttaggta ctggatgata
     9481 atcatggccg tagtctttag tccccttgcg aggcttgttc ttttcctcat ttaacttatt
     9541 ttctgcatcc ttcgcttttt tctccttttg tttccggctc tccatcgccc cggtaagtgc
     9601 agccttatct ctggataagg attcattcat tgcgtttaaa gatgcctgag ccgcttttaa
     9661 tttctcattc tgattctgaa tatccttatc aagatagcga acctgatcct gccacccgga
     9721 gccatattca tgaggcgggt tataagtcac aaaatcatct ttatttgcct ctgcctcttt
     9781 taccttttta tcggcttcct gaacagcagc ccttgcaccg gggagcgcct tctctgtcga
     9841 tgcaatgcgg ctgtttaagg tcgcaatatt tttatcttcg gcatccagct cagctttcgc
     9901 tttatcatag tctcttttca gcccctcttc cggatgagcg gcgtcccatg cctgctggcg
     9961 acgcttttct tcctcctgac gctgttttac ctgtgccggg gtaagaacat ctgtcaccga
    10021 cacataaacc ggcttctgtc cggtctcttt cgggaaacga ataacggcct cacgggagtt
    10081 accaccggct gtaaaaccag ccggacgtga atcacctttt tcagcaacaa tgccttttgg
    10141 cggggctttc gctgtcggaa cacctttcgg tacgctcacc tgcagagacg ggagacccgg
    10201 aatcgacaca ctgaataccc ccggacgttt tgtcggtttc gcatccacca cagggacact
    10261 cattggccga cctgcgacaa ccgcaatatg ctgccgctca tccttcacca catccacaac
    10321 ccgttgtctg acacgaaccg tcgcctggtc cagtggtaaa gtacttgccg gagtctccgt
    10381 caccgcatcg gccggtaatg acgtcataat ttttgacatc attttcggat catcttttgc
    10441 aatctcagaa ggcagcacac cgtagatcgc aatcccccac agaccaaact taaacggccc
    10501 cttcagggca gccagcacat cagcaacggc ggccgacaac gcatcgccgg aaactgataa
    10561 agccagtcct tcagcccccg gagtagccag ggcaggcaga ccgaaggcca tcgcagtggc
    10621 cgaagactgt ccgccaccag agctaccgct atgattgccg ctgttaccac cattaccacc
    10681 gggtttactc ccgccgttac caaactcccc cggattatag ttatgaatat ctccattcgg
    10741 cgtatgcgtc gtaccccagc ccggcccgct gtttggatcc gtaccaccac ctgatgaagg
    10801 gccaccttta ccagacgtac cgttcacatt cccactggcc tgaccaccat tatgtcccag
    10861 acctgaatta cccggacctc gaccgtctcc accactcata ccgttttcct ctttgacatt
    10921 taaaacaata aattaaagcc aacatactgt acatatacac agtataatta ttaccttact
    10981 cctcccgttt tcaggtgtca atatatgaga ttagttctca tcttcaggca caaaaaagcg
    11041 cccggagcca cctgtaagca gatcgcgtct tttcccgaaa atttctcatg gcaacaaatt
    11101 ttattattta agacattcat cacacttaca tttcagatgg tatagacaca tctgaaagct
    11161 aactggcatg aggagaggac ctgcggttaa actgttcggg aaacctgaga cgcccggagg
    11221 ccactatgac gcacctgaaa ctgactcccg ctgaagaaaa gcgcctggcg gacgcctttg
    11281 tcgctcaggc actggccatg ggcctgaaag ggaacaaaac gctgccaaag gcggtaaaac
    11341 aacaggtgcg ggaaagggtg cttcgtgcca ttcaggacga acgtgaagcc aggcgggcgc
    11401 aggaaatgcg tcgggagaaa tacgaggcta cggaaaccac cttcacctgg cagccacagc
    11461 gccgccggta aggggtattt accataacgt cgcattgccc gcaccgctca gacggtctga
    11521 gggcgtttcc ggcggctacg ggataaaaca ggcaacaaac cacccggaaa accgccggaa
    11581 acgcgcccgg taaattgtaa cccgctgcat gcctatgcac gcaactgcat ggcgggccgt
    11641 ttggtgtaaa aacgatacga taagccgtgt ttatgtaaat gaacgtggcc gtaaagagtg
    11701 acaaatatac atgtaacatt ttttactgac aggtcatcaa cccgatgcgt ttaataccct
    11761 ttatcctgct ggcattgtgc agccccttac tcctgacggc ctgtaagcat tccaccaccc
    11821 agccgccgaa aatttatact ctggataagt catctaaaat cagtagctac gctgcggcca
    11881 tcaagaccgc tgttgaaatg cagatgtttg aaccagaggc ctatgcggga aaaatgtgca
    11941 ccgtgcggat atccatacaa cgtgatggca ctctgaacag cgccattgct gaaggaggag
    12001 atccgaaact ctgtaaagca gcaatatcag ccgttaccca cgccaaaatt ccgccggcac
    12061 cggatgaaaa tacctggcaa atattcaaaa acgcccctct ggatttcaga ccatgaaagc
    12121 ttttactctg acagtgctgg cggccatgtt cctcactccg gtacatgcga taccactaaa
    12181 acccgtaacc acactgcagc caacctaccc ggtacaggcc gcagcaaaac gcatcgaagg
    12241 tcaggtgacc gccacgtttg atattctgga cgatggacga gtggacaacg tgtcgcttac
    12301 cggctgtcac cttttttttc gggaaacgct gacggcaatg aggcactggc tatatcccgc
    12361 agggcacccg gcaatgcgac aggttataaa ttttgaattc agtctgaaaa atgacggctt
    12421 acaggatccg cgaccagtca ccacccaaaa gctcaatgat gatggttgcc gccagtaaca
    12481 ggtcctttaa cccgcaggct gcaatagtcc tggatgatga gtgagcctgt agcagccctg
    12541 attagtggaa gcgaagctga aaaatgggca gcaaaagccg tatttctggc aaatagcgcc
    12601 gccacggatt aaagtagtca tgtgtaatac atatgactac aaaggggctt ttatgtcatc
    12661 agcaacatcc atcaggctcg atgaagaact taaggaccgg ctgaaaacgc tggccgatga
    12721 ccggcaccgc tcagcgcacg cgctaatgct ggaggcaatc accgaataca tcgaccggga
    12781 agagaagcgc agccagtacc tgcgggacgg acaggccgca tggcagcact atcaggaaac
    12841 gggtctgcac cttactgcgg aagaagcgga agcctggata agtacctggg gaactgagaa
    12901 cgagcaggat gctccgcc
//
