LOCUS       NZ_CP141605             5596 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP3682 plasmid pKP3682-5, complete
            sequence.
ACCESSION   NZ_CP141605
VERSION     NZ_CP141605.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38937583
            Assembly: GCF_035584575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Li,W., Zhang,Q. and Li,X.
  TITLE     Complete Genome Sequence of Klebsiella pneumoniae strain KP3682
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Li,W., Zhang,Q. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) College of Biological and Environmental
            Engineering, Binzhou University, Huanghe Road, Binzhou 256600, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. v 0.13-r308; Canu v. v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.11x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 11:41:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,845
            Genes (coding)                    :: 5,661
            CDSs (with protein)               :: 5,661
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 63 of 184
            Pseudo Genes (incomplete)         :: 127 of 184
            Pseudo Genes (internal stop)      :: 23 of 184
            Pseudo Genes (multiple problems)  :: 29 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141605.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP3682"
                     /isolation_source="Bronchoalveolar lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP3682-5"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2023-01"
                     /collected_by="Qinghuan Zhang"
     gene            complement(join(5276..5596,1..189))
                     /locus_tag="U8529_RS29785"
                     /old_locus_tag="U8529_29785"
     CDS             complement(join(5276..5596,1..189))
                     /locus_tag="U8529_RS29785"
                     /old_locus_tag="U8529_29785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<224..928)
                     /locus_tag="U8529_RS29790"
                     /old_locus_tag="U8529_29790"
                     /pseudo=""
     CDS             complement(<224..928)
                     /locus_tag="U8529_RS29790"
                     /old_locus_tag="U8529_29790"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(867..1211)
                     /locus_tag="U8529_RS29795"
                     /old_locus_tag="U8529_29795"
     CDS             complement(867..1211)
                     /locus_tag="U8529_RS29795"
                     /old_locus_tag="U8529_29795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1201..1377)
                     /locus_tag="U8529_RS29800"
                     /old_locus_tag="U8529_29800"
     CDS             complement(1201..1377)
                     /locus_tag="U8529_RS29800"
                     /old_locus_tag="U8529_29800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2017..2175
                     /locus_tag="U8529_RS29805"
                     /old_locus_tag="U8529_29805"
     CDS             2017..2175
                     /locus_tag="U8529_RS29805"
                     /old_locus_tag="U8529_29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2533..2859
                     /locus_tag="U8529_RS29810"
                     /old_locus_tag="U8529_29810"
     CDS             2533..2859
                     /locus_tag="U8529_RS29810"
                     /old_locus_tag="U8529_29810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            2885..3430
                     /locus_tag="U8529_RS29815"
                     /old_locus_tag="U8529_29815"
     CDS             2885..3430
                     /locus_tag="U8529_RS29815"
                     /old_locus_tag="U8529_29815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            3496..3846
                     /locus_tag="U8529_RS29820"
                     /old_locus_tag="U8529_29820"
     CDS             3496..3846
                     /locus_tag="U8529_RS29820"
                     /old_locus_tag="U8529_29820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(3916..4182)
                     /locus_tag="U8529_RS29825"
                     /old_locus_tag="U8529_29825"
     CDS             complement(3916..4182)
                     /locus_tag="U8529_RS29825"
                     /old_locus_tag="U8529_29825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4166..4390)
                     /locus_tag="U8529_RS29830"
                     /old_locus_tag="U8529_29830"
     CDS             complement(4166..4390)
                     /locus_tag="U8529_RS29830"
                     /old_locus_tag="U8529_29830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(5057..5269)
                     /locus_tag="U8529_RS29835"
                     /old_locus_tag="U8529_29835"
     CDS             complement(5057..5269)
                     /locus_tag="U8529_RS29835"
                     /old_locus_tag="U8529_29835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1464..1540)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          1729..2126
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3..877)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(4942..5594)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.541"
                     /coverage="99.0"
                     /evalue="1.81e-114"
ORIGIN
        1 catggccgcg ctcataccct gctcgtgggc gcggatggcg gcgctgattc tcttcccgct
       61 ttcgctcagt tccgctctga cagacttctc gtgctcgctg aatgcggctt tcagcatttc
      121 gccggtactc tgctgctgcg ctttcgattt ctgctctaag tccttcgcca gcgttaaaag
      181 actgttcata gatggctccc ttaagtcgga tgtttcgccc tccgtccggg tcggcaatgc
      241 tgatgctgct ctttgtggtg cgcacgacct caaaacctgc gctttccagc gcctcagtga
      301 cgtcctgacg cgttttaagc tcccctgacg aggcgagggc cagtaagccc cgcgtaatag
      361 cctctgcggc ttcctgcttc gttttgggta acgaagatgg cgtcaccagc gcgcgccggt
      421 tctccggtgc gttcgggtcg tgcagcccca gtcgcccgtt caccacggtc tgccacgcat
      481 cgatgcgcgg acggtctgcc cggtcgtagt acggctggag acgcctgccg gtcagcagtt
      541 ccgtgttcgg taccagaaaa ttcagttcca gccgcccctt gtcccggtgt tcaacccaca
      601 gcacgctgta ctggtcttta tcgagtccgg gcatcagaac ccgctcgaag ctcgccatca
      661 gcttttcacg ctggccgggc ggtaaatcct gttcagcaaa agacaggacg ccggaggtgt
      721 actttttggc gtagggcgag gcgtcgataa gctcccggac ctcctccggc tttccctgca
      781 ggacgatggc cccgtcgcgc tggcggtctt tccccagcag ataatccacc ggcccggcac
      841 cgccgcctcg ccctcgcgga tgaaacttaa cgatcatcgt ctgccccctt ttccagcacg
      901 gcatgccgca gccgctcgag ttcggcatca atggccatca gcgcggccac cacctgaacc
      961 cggtcagcgc ctgaccactg gccgccgtta attttccggg cgatctggtt gaggttgttc
     1021 cccatcccgg caagctggcg cagcaaaacc ggatcgatgg acggcagccg caacgaccgc
     1081 gccgggcgcg tatcgagaca ggtttcccgc atccacaccg ccagttgcct tccattgcag
     1141 cgctccagca gctgctggta ctccccatcg gtcacccgga tacctaaaaa acgggtacgc
     1201 ttaaccgcca tcctctcctc cgatatccgc aaaatgtgac cgggctgaac gctcaagctc
     1261 tgccatctgc tcccggcggt aatcttcctc ctgtgcctga cgcatcagat ttgccatcac
     1321 tgccggatgc caggatcttt tttttacgac gagcggcttc ttcttcggct tctgcattat
     1381 ccctccttcg caaacgccgg acggcacgga aaagttttcc gggtcgctcc ggcgttggac
     1441 aagcgccagc gcgtcagtgg tgttagcggg tttcggggcg cagccctgaa ccagtcacag
     1501 cgtttacgct gtgtatactg gcttactatg ttggcatgag agcacgattc atgaaagtgc
     1561 ctcatgtggc aggtgtgata tgtggctcag gagcgtccgc aataccatgc acggacagat
     1621 gaaatgacgc ctcctcgctc actcggtagc tgcgctcctg ccgttcggct gcggcgggcg
     1681 tgaccgactc acggataacg ggataaagca aaggcgctca aatcagaaag ggccggagaa
     1741 aaggatgcgg cgttgccgtt tttccatagg ctccgcctcc cctgacgagc atcacagaaa
     1801 tggacgctca agtcagagga ggacgaaacc cgacaggact taaagatgcc ggtgatttct
     1861 ctccggcggc tccctcgtgc gctctcctgt tccgaccctg ccgtttaccg gatacctgtt
     1921 ccgccttttt ccctttcggg aagcgtggcg ctttctcata gctcacgctg ctggtatctc
     1981 ggctcggttt aggtcgttcg ctccaagctg ggctgtatgc acgaaccccc cgttcagccc
     2041 gactgctgcg ccctttccgg taactatcat cttgagtcca acccggacgg acacgcaaaa
     2101 acgccactgg cagcagccat tggtaactgg atatatacga gagagatatt gagaggtctt
     2161 gaagtgggtg cctaactgcg gctacactga aagaacagtt ttggtatctg cggtctcgta
     2221 tacccagtta cccggttaag cagttcccca actgacttaa ccttcgatca aaccgcctcc
     2281 ccaggcggtt ttttcgttta cagtgcaaga gattacgcgc agaaaaaaag gatctcaaga
     2341 agatccttta aatgatcgtc agttctgtta tccccggagc tccatccatc ccacggaaaa
     2401 acacccgcca ctgatgccct cagcgcactg cacatcatgt acaaaccact ccctccctcc
     2461 ttttttattg cgtttcttaa tgcgcaatgg tagctttgtg cgcaacaaaa aacgcaatag
     2521 caaggggggg taatgagaga aagaacaaat atcatgctag atataggatt aaagcaatcc
     2581 ttacaacggc tttcgaataa aaccaaaatg agtctaagtg atcttattaa ccagagatta
     2641 tcaggttcac tttcagttga agaacgcatt ggcgaaaagc ctgaatggat gagaaacctt
     2701 gatccctatc tcgccaatca cttttcaaat attgagttac cgctcccttc accattaaaa
     2761 gaataccaag aaaaaatgga tgagatagat tcaattatta atgacatgat ggttctaaag
     2821 gaatatttca gagctaagtt ttacgacgaa ataaaatagg cacgctatac ggaggcggag
     2881 aattatgttt tttaaacggt atttaacgga gaaggaaata tacaaggcag cactcccaat
     2941 tgttaggctt caagggagag ttaacacttc attacgtttt ttatccaaat actcagatgt
     3001 aaacaaaaat ggattaattg cattgcatct taatttatta cattttatct catacaacat
     3061 acacggtgta gagtctctga aaatcactca agcaggcata aacaaagctg tacaacaaag
     3121 ccctcacgca gatgaaataa aaaatattgc gcaggcaata ggacgcaaag gacctgagta
     3181 catcgaagga ttaattagaa acccatgggg gaagaaaaat gataatccac taatatttat
     3241 aatgagcatt gaagaaagta taaaagaaac ccttaactca ggttatatgt tatatagagt
     3301 agctcccata actgagggtg aagcagctgc tttcatggat gttcttaaac aattattatt
     3361 atttgaggat aattttcgcc tttttggtag agacttggtt tataaaatag aaaaattaaa
     3421 aatcagataa caaagtatgg tgaacgaaaa atgaaagaga ggatagtgaa atgtgtaata
     3481 ccaagattct caactatgta ttggttaatt gcgttcctag catttatatt ccccggtcga
     3541 tcttttgctg acggttacac cgcttactac cgatgtaatg gtactcagga agcatctctt
     3601 actgttaaag ataacaaact aagtatggtt gtaggtcttt ctaagtttaa taggcaactg
     3661 gatacttata gattcaaaga aaagggaatc gaagtagtaa tgcagagagc tgcttctgat
     3721 gataactctg aggccataat gtcttttatt atgcctgacg ttaacaatat gaccttatta
     3781 gttaaaaata acggctacat tatcgttaag acaacctgct ggttaactaa aaccgacaaa
     3841 cagtagaaca cgtagttagt cgagtattta ccataatacc tgttgtcggc atcaaaaaag
     3901 cccgcatagc gggcttcagt cgtaaacttc gcggcgatgt ccaatcgtgg cggcaaggat
     3961 aacaagctcg tcatctcgga tctcacacag aacgcgataa tctcccaccc gatagcgcca
     4021 gaactcgccc agctcacctt tgagcggctt ccctgactgg cgaggatcgg cagcaactgc
     4081 aatgcgtaaa ctcataaaat ccacaatccg tcgtgcgttc tgtttgtcca tcttgcgtaa
     4141 cgatttgagc gcccgatcgg aatagttaat cgtccagacc aagtgacttc ctcacgtctt
     4201 cagacgaatg cactgcttca tcaccacggc gaacgcgtgc agcagtttct gctgaaagat
     4261 aataatcctc caggtcttcc agatgcgcca gtattgcttc ccgcgcataa aacgtctttg
     4321 ttctgccggt ttgcttcgcc agcgagtcca gacgggactc aatctcatca gataaccgga
     4381 tagcaagcat ataaacctcc cgaacaccat ttgctataca tgtatatcaa cagtaccaca
     4441 atcaacctaa aagaacaaaa aataatcttg ttagcccctc ttgataatcc gtaacgcggt
     4501 caatatatct agcttgaggg ctttcctctt aactattgaa aggataacga ttatgggtct
     4561 taagccgggt ccgaaacgaa ttgccgattc aacaggggaa cctgataaac gccagcgtga
     4621 caataaaaaa acgccgggaa atactgacaa attgaaaccc agcaaatctt caaaaaaata
     4681 agtcagtagt taaaagccct ccggggcttt tttgtatctg ccgttcacgc tgattaaaca
     4741 taacacccgt tgcccgcacc gccccggacg ggttcgccgc gaacggggtg gcagtcaggt
     4801 aaaaccggca acaaaccacc cgataaaccg ccccgttcgc gacagataaa ttttacctgg
     4861 ctgcatagcc atgcagccag gtgaatgaca tccggtttca gcgtaaatgc ccgatgagaa
     4921 ccctgttttc ctgcatttca gcccagcgac ggaccgtcat agcttcgctg ccgggaacgc
     4981 gcttccgtca gttccttttc gtgctgccgt tgctcgtgcg cccgttctgc ggccacggcc
     5041 atctcatgac gctgcactac cggctcaaac gctgcagttg cccgctcaag cgttccacct
     5101 gctgactcaa gcgcgtcacg cgctcgctca gcgccgcgtt ctcccgttgc ataagccctg
     5161 acattttccg ccattccccg aaggcgctct cccactcgtc cagccttttc gagtagtcct
     5221 gctgtagctg ctctaatgcg ctcagcaact gtttttccag ctcggtcatc ggcacttact
     5281 ccttcaccag tctcagcgcg ttctgcttcg accgcccgtt cccgaccgtc cagctctgcg
     5341 tcctgtctac ggccgtcccc ttcggcagca ccagaaaacg tccgttgctg tcttccaggt
     5401 acgtcacgcc ccaggtcttc gcgttcagct tcgacagcgc tgcgttctgc cggtcgattt
     5461 cggccagatt tgacgctatc aagctcccct gataccacaa caccccgctc aggctggcga
     5521 acagcagtcc tgacaccatc gtgatggtca gccatgtccg cccgaccata cgcatcacgc
     5581 tcaggcggtt cgactg
//
