LOCUS       NZ_CP141604            11970 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP3682 plasmid pKP3682-4, complete
            sequence.
ACCESSION   NZ_CP141604
VERSION     NZ_CP141604.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38937583
            Assembly: GCF_035584575.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Li,W., Zhang,Q. and Li,X.
  TITLE     Complete Genome Sequence of Klebsiella pneumoniae strain KP3682
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Li,W., Zhang,Q. and Li,X.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2023) College of Biological and Environmental
            Engineering, Binzhou University, Huanghe Road, Binzhou 256600, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Hifiasm v. v 0.13-r308; Canu v. v. 1.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38.11x
            Sequencing Technology             :: Illumina NovaSeq; PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 11:41:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,845
            Genes (coding)                    :: 5,661
            CDSs (with protein)               :: 5,661
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 184
            CDSs (without protein)            :: 184
            Pseudo Genes (ambiguous residues) :: 0 of 184
            Pseudo Genes (frameshifted)       :: 63 of 184
            Pseudo Genes (incomplete)         :: 127 of 184
            Pseudo Genes (internal stop)      :: 23 of 184
            Pseudo Genes (multiple problems)  :: 29 of 184
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141604.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP3682"
                     /isolation_source="Bronchoalveolar lavage fluid"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP3682-4"
                     /geo_loc_name="China:Zhuhai"
                     /lat_lon="22.28 N 113.58 E"
                     /collection_date="2023-01"
                     /collected_by="Qinghuan Zhang"
     gene            join(11558..11970,1..1273)
                     /locus_tag="U8529_RS29715"
                     /old_locus_tag="U8529_29715"
     CDS             join(11558..11970,1..1273)
                     /locus_tag="U8529_RS29715"
                     /old_locus_tag="U8529_29715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1283..1540
                     /locus_tag="U8529_RS29720"
                     /old_locus_tag="U8529_29720"
     CDS             1283..1540
                     /locus_tag="U8529_RS29720"
                     /old_locus_tag="U8529_29720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            1625..1774
                     /locus_tag="U8529_RS29725"
                     /old_locus_tag="U8529_29725"
     CDS             1625..1774
                     /locus_tag="U8529_RS29725"
                     /old_locus_tag="U8529_29725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3258..5222
                     /locus_tag="U8529_RS29730"
                     /old_locus_tag="U8529_29730"
     CDS             3258..5222
                     /locus_tag="U8529_RS29730"
                     /old_locus_tag="U8529_29730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5222..5953
                     /gene="mobC"
                     /locus_tag="U8529_RS29735"
                     /old_locus_tag="U8529_29735"
     CDS             5222..5953
                     /gene="mobC"
                     /locus_tag="U8529_RS29735"
                     /old_locus_tag="U8529_29735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            5960..6490
                     /locus_tag="U8529_RS29740"
                     /old_locus_tag="U8529_29740"
     CDS             5960..6490
                     /locus_tag="U8529_RS29740"
                     /old_locus_tag="U8529_29740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6517..6696)
                     /locus_tag="U8529_RS29745"
                     /old_locus_tag="U8529_29745"
     CDS             complement(6517..6696)
                     /locus_tag="U8529_RS29745"
                     /old_locus_tag="U8529_29745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6722..7150)
                     /locus_tag="U8529_RS29750"
                     /old_locus_tag="U8529_29750"
     CDS             complement(6722..7150)
                     /locus_tag="U8529_RS29750"
                     /old_locus_tag="U8529_29750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(7314..7628)
                     /locus_tag="U8529_RS29755"
                     /old_locus_tag="U8529_29755"
     CDS             complement(7314..7628)
                     /locus_tag="U8529_RS29755"
                     /old_locus_tag="U8529_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7616..7894)
                     /locus_tag="U8529_RS29760"
                     /old_locus_tag="U8529_29760"
     CDS             complement(7616..7894)
                     /locus_tag="U8529_RS29760"
                     /old_locus_tag="U8529_29760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(8069..8434)
                     /locus_tag="U8529_RS29765"
                     /old_locus_tag="U8529_29765"
     CDS             complement(8069..8434)
                     /locus_tag="U8529_RS29765"
                     /old_locus_tag="U8529_29765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(8431..8802)
                     /gene="tolA"
                     /locus_tag="U8529_RS29770"
                     /old_locus_tag="U8529_29770"
     CDS             complement(8431..8802)
                     /gene="tolA"
                     /locus_tag="U8529_RS29770"
                     /old_locus_tag="U8529_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(9076..9321)
                     /locus_tag="U8529_RS29775"
                     /old_locus_tag="U8529_29775"
     CDS             complement(9076..9321)
                     /locus_tag="U8529_RS29775"
                     /old_locus_tag="U8529_29775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9966..11453
                     /gene="ltrA"
                     /locus_tag="U8529_RS29780"
                     /old_locus_tag="U8529_29780"
     CDS             9966..11453
                     /gene="ltrA"
                     /locus_tag="U8529_RS29780"
                     /old_locus_tag="U8529_29780"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             1..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1878..2456)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5223..5950
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tggtctgtgg gggattgcga tctacggtgt gctgccttct gagattgcaa aagatgatcc
       61 gaaaatgatg tcaaaaatta tgacgtcatt accggccgat gcggtgacgg agactccggc
      121 aagtacttta ccactggacc aggcgacggt tcgtgtcaga caacgggttg tggatgtggt
      181 gaaggatgag cggcagcata ttgcggttgt cgcaggtcgg ccaatgagtg tccctgtggt
      241 ggatgcgaaa ccgacaaaac gtccgggggt attcagtgtg tcgattccgg gtctcccgtc
      301 tctgcaggtg agcgtaccga aaggtgttcc gacagcgaaa gccccgccaa aaggcattgt
      361 tgctgaaaaa ggtgattcac gtccggctgg ttttacagcc ggtggtaact cccgtgaggc
      421 cgttattcgt ttcccgaaag agaccggaca gaagccggtt tatgtgtcgg tgacagatgt
      481 tcttaccccg gcacaggtaa aacagcgtca ggaggaagaa aagcgtcgcc agcaggcatg
      541 ggacgccgct catccggaag aggggctgaa aagagactat gataaagcga aagctgagct
      601 ggatgccgaa gataaaaata ttgcgacctt aaacagccgc attgcatcga cagagaaggc
      661 gctccccggt gcaagggctg ctgttcagga agccgataaa aaggtaaaag aggcagaggc
      721 aaataaagat gattttgtga cttataaccc gcctcatgaa tatggctccg ggtggcagga
      781 tcaggttcgc tatcttgata aggatattca gaatcagaat gagaaattaa aagcggctca
      841 ggcatcttta aacgcaatga atgaatcctt atccagagat aaggctgcac ttaccggggc
      901 gatggagagc cggaaacaaa aggagaaaaa agcgaaggat gcagaaaata agttaaatga
      961 ggaaaagaac aagcctcgca aggggactaa agactacggc catgattatc atccagtacc
     1021 taaaacagaa gacataaagg gacttggaga gttaaagcga ggtgatccta aaacacctaa
     1081 acaaggtggt ggtggtaagc gggcccgatg gtatggcgat aaaaaacgta aaatttatga
     1141 atgggattcc cagcacggtg agcttgaagg gtatcgtgcc agtgatggcg aacacctagg
     1201 cgcatttgat cccaaaacgg gtaagcaggt taaagggcca gatccaaaac ggaacattaa
     1261 aaaatatctt tgagaggtaa atatggggct taaattacgt ttgacatggt ttgataagaa
     1321 aactgaagag tttaaagggg aagagtattc taaagacttt ggtgacgatg gttctgtcat
     1381 tgaaagcttg ggaatgcctt taaaggataa tattaataat ggtgagtttg atttgaagaa
     1441 agaatgggtt cctttactac agccattttt taaaaatcaa ataaaacctg atgtggataa
     1501 ttgttatata gcttttgatt atcgggacgg taactggtaa caaaaaatta tcaaggatga
     1561 tttgaaatac tgacttccgg tgttcaggga tgaatgccgg agtctttaaa tacagggaaa
     1621 gtatatgaaa aaggtaaaga ctatttttct ttttatattg attgttgctg gctttcttct
     1681 ggccgcatgt caggcaaatt atatccggga tgttcagggc ggaacggtgg caccatcgtc
     1741 ctcctctgaa ctgacgggga tcgcggttca gtagaaaaga tcaaaggatc ttcttgagat
     1801 cctttttttc tgcgcgtaat cttttgccct gtaaacgaaa aaaccacctg gggaggtggt
     1861 ttgatcgaag gttaagtcag ttggggaact gcttaaccgt ggtaactggc tttcgcagag
     1921 cacagcaacc aaatctgtcc ttccagtgta gccggacttt ggcgcacact tcaagagcaa
     1981 ccgcgtgttt agctaaacaa atcctctgcg aactcccagt taccaatggc tgctgccagt
     2041 ggcgttttgc gtgctttccc gggttggact caagtgaaca gttaccggat aaggcgcagc
     2101 agtcgggctg aacggggagt tcttgcttac agcccagctt ggagcgaacg acctacaccg
     2161 agccgagata ccagtgtgtg agctatgaga aagcgccaca cttcccgcaa gggagaacgg
     2221 cggacaggta tccggtaaac ggcagggtcg gaacaggaga gcgcaagagg gagcgaccta
     2281 ccggaaacgg tggggatctt taagtcctgt cgggtttcgc ccgtactgtc agattcatgg
     2341 ttgagcctca cggctcccac agatgcaccg aaaaagcgtc tgtttatgtg aactctggca
     2401 ggagggcgga gcctatggaa aaacgccacc ggcgcggccc tgccgttttg cctcacatgt
     2461 tagtcccctg cttatccacg gaatctgtgg gtaactttgt atgtgtccgc agcgcccgcc
     2521 gcagtctcac gcccggagcg tagcgaccga gtgagcgagg aggcgcaatt tccccggcac
     2581 ctgcaagacg gtcaccggac gcgctgatgt gggatattgt cccctgatta agtctttcta
     2641 ttccctgacg atcaattatc aggataggtg aaccatgtcg gtttttcgct gaacatttta
     2701 cgttaaccta tctctgttgc ttttttctcc ttgcggagaa atgcaagtca tccccgctgg
     2761 ggcgtggctg acttcagggg aaaagacctt gcttacgctg gctggacgcc accgacgcac
     2821 ggtcttttcc cctctttctg gctctgtccg gctggacgcc ggccacgcca gaaattcacc
     2881 cggcccccgt caggctcacg cctgaccacc cacaaaaatc cacggtgctg gacgcacctg
     2941 acgaagcagc aaagctgctc ctgattttgc gggtggtcag ccgctcgcta tggcgaactc
     3001 tgacgggggc cggaacaccg gaaaagaccg cactggggtg ccgccttttc ctctcacacg
     3061 ctggggcgtg ctcgttgttc ctccggggct gcgccttttc cgcatggggc ggaaagaggc
     3121 tggcctccgc ggtgaaagct gaacgcaatc accacggacg tgaccgccgc cgccatgcct
     3181 tggggcatgt ccgcggcttg tgcagcgggg gctgcgcatc acgccggtaa acccgtcgtc
     3241 tgaaggagag agattgcatg tttaatacgg attcgcttgc ctggcagtgg catatcccgg
     3301 agctggcccg gcagatttac ccgtgggtca gctggctgta ccgggagtat tacagtctgg
     3361 ccggtttttt tctggttatc ggcctgtttt tcggtctggc tgaactctgg gccggccgaa
     3421 tgcgttttgt ggggctgagc cataaggcgc ggcgggagta tgccgatcgc tataagcgcg
     3481 ataataaccg gactgtgcca ttttcggtgc tggcctcgct ggattcaccc gggcggatgg
     3541 tcagggcacg gcgacatgtc tggaactacg cgtactggct gaccggcgct gccggtctgg
     3601 cccttctggg ggggctggtt gtcgccgtgt ttatcgagag ctggggagaa accgtcctgc
     3661 gcccggacct ctatgcgaac gcagattatt acctgctgca gccggctaac ctgttcggga
     3721 tagcctggtg tgccggaggg cttgtcgccg gcgtccttct ggcgcggctg ctggcgttcc
     3781 tgctgcttga tggtcatttt gcggccgctg acgaggcggt gaacgcgaaa ctgaatcagg
     3841 agagccggcg cagcagccag cggaccggag aaatgacaga cgtccgccac ctgcatttcg
     3901 gggagccggt accggttaac gcgctggccg atttttccac ggagcaggcc cggaaacagc
     3961 aggcggtgtt tctgggtaag gatgagcagg gccagccggt gctggtccct cgtgatacct
     4021 ggcgcaagac caatattcag atcctcggcc tgcccgggag cggcaagagc gcgatggcca
     4081 ccaacgcgct cattcgctgc gtacgcgatt tcggggacgc tgtggtgtac ttcgacccca
     4141 aaggggacgc gtgggcgccg cacgtcttcc gggcgcactg cccggacttc accctgctgg
     4201 atctgcggcc ggggaaaccg gcgcagctga atctgttccg cgatctcgac cagtacgccc
     4261 tgaagaatct gctggttgcc gggtttaacc tgagtgagac cggcgacgtg gcggaccact
     4321 accgcatcag cgagcagaaa gcggcgaagc tgattgcgga gcagttcccg cagggggcaa
     4381 acattcagca ggtgctggcg gcggcgtacg cgttgccgga ggccctgaaa aaggacgtga
     4441 aggggctgat caccaagctg gagaacgtgg ccgacctgag cgtcctgcag acggacagcg
     4501 gtatcgatgt ggcggggata atcaacggcg gcggctgcct gtacgtcatc ggctcgatgg
     4561 acgatgaggc ggtgatccgg gtgcagaaga tgctgtttgc ccgctgcgcg caaatcatca
     4621 ttgcgcggga tgagttccgg aggtggccgc acgcgagcat catgctcgat gagattaaat
     4681 atctgctgtc gaagtacgtg ctgaacgcgc tggggacgct gcgcagccgg gactgcaacc
     4741 tgctgctcgc gcaccagtcg ctgggcgact tcggtcagtg cgggcaggat ctgcccgctg
     4801 attttgtgaa aaccacggta ctggataaca cgccgatccg ctggttctat cgcgcggcca
     4861 gccaggagtc cgcgcagtgg gcggcgggcc agaccgggga gatccgggtg gacgtggagc
     4921 gccgccgggc cagccgggag gcggggaacg tggagcacat cagcggcgac agcttcatcc
     4981 agaaggaggc gcggccgctg tttgacgtga acaccctgca acacctgcct gacgggttcg
     5041 cggtgatgac cgggctgggc gtggcgcggc tggcgttcag cagcccgctg cgggttgagc
     5101 gccgggaaat ttcgctgaaa accttcccgg tgctggcgaa aacggacccg ctggcggagt
     5161 acctgcctcc ggccgacggc ggcggaccaa aagcggatga tgattttgcg gggctttact
     5221 gatgacactg gagcagtaca acgtcagcca cgcgaagcgg caggcgcgga acgcggagaa
     5281 aacacggctg accctgcgct ggctgcggga agagctgtgt tccacggcgg agctggttgc
     5341 gcgacggctg gggattgcgg cggtacagcc ggtttaccgc ttcctggaca gtctggtggc
     5401 gaaagggctg ctggtccggg cgaaataccc ggttgacggc cgtcaggtca gcgtgtgggg
     5461 gctgacgccg cacggggtgg cgttctcgtt cgacgaggac gagccgctga cggacgttat
     5521 tccgtttcag ccgtcaaggg tgtcggcggc gcagctgccg caccggctgg ccgtgcagtc
     5581 cctgcgcctc tccatggagg gccggggggc caccggctgg cggtacctgc accgtatggc
     5641 cctgaaaggg atgaaagtgc cggacgcgct ggcggagctg gacggcagaa cggtggcatt
     5701 tgaggtagag cgcaccgtca agagccgcag gcggtatcag gaagtggtgt ccgggtatct
     5761 gttcaaccgc cgggcgaacg gcatcgatga aatctggtac atctgccctg accggccgac
     5821 gcttgtccgg gtacagcggg cgattctggc ggtggatgaa atcgtgaatc cacagaccgg
     5881 cgaggcccgc aaaacggtgg agctggaccg ggagaggctg ttcgcgtgct ttaaatttat
     5941 gacaacggag tgagggatta tggcgctgta catggtattt attgaggacg tggaagacaa
     6001 agacggcgag cgctgcatta tgccaaccag cgagcatctg gaagacatgg tggtgctcgc
     6061 ctggcagacc ggggcgtatg ttttctgttt tgtcgaccgg gcccagcatc tggcgatgat
     6121 gaaatccatc gggatgtatg acgagcggct caacagcgac gaaaaagcca cgtacttttt
     6181 caccggtgaa aatccgcaat gggaacggga caaacccaca ggaatgaagc tgtgtgatta
     6241 cctctatcga aaggtggagc ggttcgccat cgagaacggt gtgcaggtgg ttgatgccgg
     6301 gaagtgctca ccggagcaga ttaacgcgat gattgaggtg gtcagcgagc ggcacgggga
     6361 aagcgcggtg aacgagaagc tgaaagcgga gctgatgcgg gaatataaca gccgctattt
     6421 taacgtgccg gtgcctgaac tgccggagga caaacccgcc cgccggtggt ggccgttcgg
     6481 caaaaaataa cgccagcacc ggcatggccg gtgctgttat tcttccccga gcttatcccg
     6541 catcacctgc cagagtgttt ccgcatcctc gtgaagccgt tcgcacatgt cggccggggt
     6601 atccatgtcc agctcgttca gcttttccag caacagcagc gactgatcct taatgaattt
     6661 agccatattg agggcagttt tttcctgctt cgtcatgttc tccattccct tcggtaagtt
     6721 ctcagctaaa ccagccggac cagaagcgtt ttttcttctg tgtcagttct gttgtcaatc
     6781 tgtccacttc ggcctggagt ttccggcgct catcatcgtg ctggcggcgt tcttcttccc
     6841 gggatgtttt atcttccagc atcagggtga cagcctgccg gagatcggcc agttcctttt
     6901 gcattgccag aagctgttcc tgtgggaaat ttttttgtgt attttcacgg tgtgaaatgc
     6961 cgggaatttg tttctcaggg atattcacat ttccgtatac acggatcagt tctgacacat
     7021 ccacgacagg gttgtttttc ccgtcacgtg acacggtaac ctttccctgt tttacatgat
     7081 tgtatagcgt tcttctggta atgcccgcgg cttgtgctgc ctgtgaaagg ttgagcaatg
     7141 ttttcgccac tgtatacccc tgattttatc gggtgtgaaa ggtgtgtaat ttctcacggt
     7201 aaatgtatcg ctgaatttta cccgtttttc cgttcaggat gtggttttct gacgcgctgc
     7261 gcttgtcaaa ccggcgacgg ccacgcaatg aattgcgccc ccgtcatcgg ttattagagg
     7321 ctgtctgccc ccggataccc ggattctttc tgatgcctga cggtgacgat cacaatctgg
     7381 tccatttccc ggtcgtggcg gtagagcatc acatagccac tgtcgccaaa cccgatcacc
     7441 agttccttat attccagcgg cagaaacggc accgggcggc caatgtctgg caatgttttc
     7501 agttgctgga tagcccggac gatcacctca cctccttttc tggctgccag ccgatttttg
     7561 gttttgagaa agtcctgaag gcgttgtaaa tcgcgctgtg ccagcgcgga aatcgttacc
     7621 tgtggcatgg cggagcatcc tgctcgttct cagttcccca ggtacttatc caggcttccg
     7681 cttcttccgc agtaaggtgc agacccgttt cctgatagtg ctgccatgcg gcctgtccgt
     7741 cccgcaggta ctggctgcgc ttctcttccc ggtcgatgta ttcggtgatt gcctccagca
     7801 ttagcgcgtg cgctgagcgg tgccggtcat cggccagcgt tttcagccgg tccttaagtt
     7861 cttcatcgag cctgatggat gttgctgatg acataaaagc ccctttgtag tcatatgtat
     7921 tacacatgac tactttaatc cgtggcggcg ctatttgcca gaaatacggc ttttgctgcc
     7981 catttttcag cttcgcttcc actaatcagg gctgctacag gctcactcat catccaggac
     8041 tattgcagcc tgcgggttaa aggacctgtt actggcggca accatcatca ttgagctttt
     8101 gggtggtgac tggtcgcgga tcctgtaagc cgtcattttt cagactgaat tcaaaattta
     8161 taacctgtcg cattgccggg tgccctgcgg gatatagcca gtgcctcatt gccgtcagcg
     8221 tttcccgaaa aaaaaggtga cagccggtaa gcgacacgtt gtccactcgt ccatcgtcca
     8281 gaatatcaaa cgtggcggtc acctgacctt cgatgcgttt tgctgcggcc tgtaccgggt
     8341 aggttggctg cagtgtggtt acgggtttta gtggtatcgc atgtaccgga gtgaggaaca
     8401 tggccgccag cactgtcaga gtaaaagctt tcatggtctg aaatccagag gggcgttttt
     8461 gaatatttgc caggtatttt catccggtgc cggcggaatt ttggcgtggg taacggctga
     8521 tattgctgct ttacagagtt tcggatctcc tccttcagca atggcgctgt tcagagtgcc
     8581 atcacgttgt atggatatcc gcacggtgca catttttccc gcataggcct ctggttcaaa
     8641 catctgcatt tcaacagcgg tcttgatggc cgcagcgtag ctactgattt tagatgactt
     8701 atccagagta taaattttcg gcggctgggt ggtggaatgc ttacaggccg tcaggagtaa
     8761 ggggctgcac aatgccagca ggataaaggg tattaaacgc atcgggttga tgacctgtca
     8821 gtaaaaaatg ttacatgtat atttgtcact ctttacggcc acgttcattt acataaacac
     8881 ggcttatcgt atcgttttta caccaaacgg cccgccatgc agttgcgtgc ataggcatgc
     8941 agcgggttac aatttaccgg gcgcgtttcc ggcggttttc cgggtggttt gttgcctgtt
     9001 ttatcccgta gccgccggaa acgccctcag accgtctgag cggtgcgggc aatgcgacgt
     9061 tatggtaaat accccttacc ggcggcgctg tggctgccag gtgaaggtgg tttccgtagc
     9121 ctcgtatttc tcccgacgca tttcctgcgc ccgcctggct tcacgttcgt cctgaatggc
     9181 acgaagcacc ctttcccgca cctgttgttt taccgccttt ggcagcgttt tgttcccttt
     9241 caggcccatg gccagtgcct gagcgacaaa ggcgtccgcc aggcgctttt cttcagcggg
     9301 agtcagtttc aggtgcgtca tagtggcctc cgggcgtctc aggtttcccg aacagtttaa
     9361 ccgcaggtcc tctcctcatg ccagttagct ttcagatgtg tctataccat ctgaaatgta
     9421 agtgtgatga atgtcttaaa taataaaatt tgttgccatg agaaattttc gggaaaagac
     9481 gcgatctgct tacaggtggc tccgggcgct tttttgtgcc tgaagatgag aactaatctc
     9541 atatattgac acctgaaaac gggaggagta aggtaataat tatactgtgt atatgtacag
     9601 tgtgcgccgg gagaccggtt agagatcaag gggtgaaagt ccccgaccat tgaaggacca
     9661 gcaatccaca aggtccccga gtcatgcgtt gcataccgcg aggtatgggg cgaagcgttg
     9721 acaggggtgt tgacaggcca gccattgagc cacgaaatgt atattaaatt accgggtgcc
     9781 gacgttgtac tgttaacgga aggcaacatc atagggtgcg acactgcgag tgccacatgg
     9841 acccggcggg gtctgagacc ctggcatgtc aatacgatct ctacgcggga accgggagat
     9901 ctcccctctg accatctgcc agtgtcggag atggcccgca ccgggaagac gaggagtcat
     9961 agccggtgat gtacggagag gagaagtcgg actcgctcat agtagcggcg aagcaggcga
    10021 acaacccgaa aggagcggag tcagtggagc gaaggagcgg ggccaagggg aacgcggaac
    10081 agccacacat gcgccggaca cagagccggg aaagcatgtc acagaggctg tcacgcgtgc
    10141 gggaagctgc gaagcagcgg aagaaagaac ggtttacagc attgttccac ctgctgacag
    10201 tcgaagcact ggaagccgca ttcctctccc tgagcaggaa agcggccgcc ggagtggatg
    10261 gcatcaggtg gatggactac gccggaaaca tgaagaacaa cataacagat ctgcaccgga
    10321 ggctacatca gggcagctac agggcgcagc ccggcaggcg tcactacatc ccaaaagcgg
    10381 atggaaaaca acgcccgctc ggcatcgcct cgctggagga caagatcgtc cagtatgcgc
    10441 tggtgaaaat cctgaacgca gtctatgaaa acgactttat ggggttctca tacgggttca
    10501 gacccgggcg aagccagcac gatgcactgg acgcactggc cacagggctg gtacgcacta
    10561 acgtaaactg ggtactggat gccgacatca gtcagttctt cgacagggtg agccacgaat
    10621 ggctgatcag gttcacagag catcggatcg gcgaccggag ggtaatcagg ctcatacgta
    10681 agtggctcac agccgggacg tcggaggagg gtcaatggcg agcaacggag gaaggcaccc
    10741 cacagggtgc ggtcatctca ccgctgctgg caaacatata cctccactac gtcttcgatc
    10801 tgtgggcgca tcagtggcga cgtcgctatg ccacaggcaa tgtggtaatg gtcagatacg
    10861 ccgatgacat cgtcatcggg ttcgacaaac gatacgatgc ccggcgcttc cgtatagcca
    10921 tgcagcgcag actgagggag ttcggactca cggttcaccc ggagaaaacc cgtctgatgg
    10981 agttcggccg cttcgctgcc gaaaaccgtg ccatcagggg aaaaggcaaa ccagaaacgt
    11041 tcaacttcct cgggttcacg cacatcagcg ggaaagatcg caacggcagg ttcatgctga
    11101 tacgaaagac ccgccgggat cggatgacgg caactctgaa agccatcaaa gacggtctgc
    11161 gaaggcgctg gcattactca atccccgaac agggaaaatg gctcaggaga gtggttcagg
    11221 gatacctgaa ctatcactcg gtaccgggca acttccccac catgcagaag ttcaggacac
    11281 acgtaacaaa cctctggcgc cgggcgctca ggcgcaggag ccagaaggat gatacgacct
    11341 ggacgaaagc aaacaaactg gcagccgcat ggctaccaag ggttcgggtt cttcatccat
    11401 ggcctgtgga gcggttcacc gccagacacc cgaggcagga gcccggtgcg taaatcgcgc
    11461 acgccgggat ctgtgcgggg ggtatccggt aacgggtatc cctaccgcga catgttggct
    11521 ttaatttatt gttttaaatg tcaaagagga aaacggtatg agtggtggag acggtcgagg
    11581 tccgggtaat tcaggtctgg gacataatgg tggtcaggcc agtgggaatg tgaacggtac
    11641 gtctggtaaa ggtggccctt catcaggtgg tggtacggat ccaaacagcg ggccgggctg
    11701 gggtacgacg catacgccga atggagatat tcataactat aatccggggg agtttggtaa
    11761 cggcgggagt aaacccggtg gtaatggtgg taacagcggc aatcatagcg gtagctctgg
    11821 tggcggacag tcttcggcca ctgcgatggc cttcggtctg cctgccctgg ctactccggg
    11881 ggctgaagga ctggctttat cagtttccgg cgatgcgttg tcggccgccg ttgctgatgt
    11941 gctggctgcc ctgaaggggc cgtttaagtt
//
