LOCUS       NZ_CP141577             9310 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP32_L7_41.22_ST86 plasmid unnamed,
            complete sequence.
ACCESSION   NZ_CP141577
VERSION     NZ_CP141577.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38771709
            Assembly: GCF_035599035.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9310)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9310)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: APR-2023
            Assembly Method :: flye v. 2.9.1-b1780; Medaka v. 1.7.2
            Assembly Name                     :: q20run12_bc17
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 132x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 12:03:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,326
            CDSs (total)                      :: 5,202
            Genes (coding)                    :: 5,063
            CDSs (with protein)               :: 5,063
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 75 of 139
            Pseudo Genes (incomplete)         :: 67 of 139
            Pseudo Genes (internal stop)      :: 25 of 139
            Pseudo Genes (multiple problems)  :: 26 of 139
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141577.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9310
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP32_L7_41.22_ST86"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-09-28"
                     /genotype="ST86"
     gene            complement(44..595)
                     /locus_tag="VI615_RS26545"
                     /old_locus_tag="VI615_26545"
     CDS             complement(44..595)
                     /locus_tag="VI615_RS26545"
                     /old_locus_tag="VI615_26545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_267668043.1"
                     /translation="MARIHAIKRSGLHIDIDYVVHKKIALDRAVILRYSTKNPDYHADF
                     VGLSEPEIEREKDLILKENDTTHCLLLLTAIEAIFRLDADQKYCNKKRDPLSRKIKEIY
                     DKTKGEKIKLDDDLINTRMNEDDARLKKDYNDLKTCFKFRHWLAHGRYWHPKRIKNFYF
                     DSILIVIDSILNSTKYNFEV"
                     /name="hypothetical protein"
     gene            complement(588..875)
                     /locus_tag="VI615_RS26550"
                     /old_locus_tag="VI615_26550"
     CDS             complement(588..875)
                     /locus_tag="VI615_RS26550"
                     /old_locus_tag="VI615_26550"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_099459482.1"
                     /translation="MLIGSKGRVDVTGPFGKEKFILIRKGVKSPSDLIKVRVNVVGAYK
                     PEKDKTDEDVKKPSIDDWEWKTMPSDSRWVKFDDVNSETITNIIMRMING"
                     /name="hypothetical protein"
     gene            complement(962..1153)
                     /locus_tag="VI615_RS26555"
                     /old_locus_tag="VI615_26555"
     CDS             complement(962..1153)
                     /locus_tag="VI615_RS26555"
                     /old_locus_tag="VI615_26555"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_324696315.1"
                     /translation="MNMKTLKDIIEKQKKEEEEQKFDPAEQIEEYISLVEKLYTITLES
                     IDELIKDGLIKQSRKKYQ"
                     /name="hypothetical protein"
     gene            complement(1420..1533)
                     /locus_tag="VI615_RS26560"
                     /old_locus_tag="VI615_26560"
     CDS             complement(1420..1533)
                     /locus_tag="VI615_RS26560"
                     /old_locus_tag="VI615_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072013440.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1949..2140
                     /locus_tag="VI615_RS26565"
                     /old_locus_tag="VI615_26565"
     CDS             1949..2140
                     /locus_tag="VI615_RS26565"
                     /old_locus_tag="VI615_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044525164.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2140..2541
                     /locus_tag="VI615_RS26570"
                     /old_locus_tag="VI615_26570"
     CDS             2140..2541
                     /locus_tag="VI615_RS26570"
                     /old_locus_tag="VI615_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3326..3401)
                     /locus_tag="VI615_RS26575"
                     /old_locus_tag="VI615_26575"
     tRNA            complement(3326..3401)
                     /locus_tag="VI615_RS26575"
                     /old_locus_tag="VI615_26575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3366..3368),aa:Arg,seq:tct)
                     /name="tRNA-Arg"
     gene            complement(3582..3824)
                     /locus_tag="VI615_RS26580"
                     /old_locus_tag="VI615_26580"
     CDS             complement(3582..3824)
                     /locus_tag="VI615_RS26580"
                     /old_locus_tag="VI615_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074196216.1"
                     /translation="MSKESELEFRTKELERQMKGVQRRIEVVNAKYDSQTKKQERRIRD
                     LEIKTAVQSGVTQREVANIYELSPGRVNQIIKKVG"
                     /name="hypothetical protein"
     gene            4214..4609
                     /locus_tag="VI615_RS26585"
                     /old_locus_tag="VI615_26585"
     CDS             4214..4609
                     /locus_tag="VI615_RS26585"
                     /old_locus_tag="VI615_26585"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126066683.1"
                     /translation="MNANEVKDILEHSNHDDWIVDDETGSFTYKNDLNLHIKRADYDNY
                     NSFSEPWAVSHPNPNACSVQYTVNYGSSFVDRKTLVSVDGHRATLPMPASANDLTVTSA
                     DVNFAKIVDTGERVDEYILRSGLKVQP"
                     /name="hypothetical protein"
     gene            complement(4699..5250)
                     /locus_tag="VI615_RS26590"
                     /old_locus_tag="VI615_26590"
     CDS             complement(4699..5250)
                     /locus_tag="VI615_RS26590"
                     /old_locus_tag="VI615_26590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_267668043.1"
                     /translation="MARIHAIKRSGLHIDIDYVVHKKIALDRAVILRYSTKNPDYHADF
                     VGLSEPEIEREKDLILKENDTTHCLLLLTAIEAIFRLDADQKYCNKKRDPLSRKIKEIY
                     DKTKGEKIKLDDDLINTRMNEDDARLKKDYNDLKTCFKFRHWLAHGRYWHPKRIKNFYF
                     DSILIVIDSILNSTKYNFEV"
                     /name="hypothetical protein"
     gene            complement(5243..5530)
                     /locus_tag="VI615_RS26595"
                     /old_locus_tag="VI615_26595"
     CDS             complement(5243..5530)
                     /locus_tag="VI615_RS26595"
                     /old_locus_tag="VI615_26595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_099459482.1"
                     /translation="MLIGSKGRVDVTGPFGKEKFILIRKGVKSPSDLIKVRVNVVGAYK
                     PEKDKTDEDVKKPSIDDWEWKTMPSDSRWVKFDDVNSETITNIIMRMING"
                     /name="hypothetical protein"
     gene            complement(5617..5808)
                     /locus_tag="VI615_RS26600"
                     /old_locus_tag="VI615_26600"
     CDS             complement(5617..5808)
                     /locus_tag="VI615_RS26600"
                     /old_locus_tag="VI615_26600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_324696315.1"
                     /translation="MNMKTLKDIIEKQKKEEEEQKFDPAEQIEEYISLVEKLYTITLES
                     IDELIKDGLIKQSRKKYQ"
                     /name="hypothetical protein"
     gene            complement(6075..6188)
                     /locus_tag="VI615_RS26605"
                     /old_locus_tag="VI615_26605"
     CDS             complement(6075..6188)
                     /locus_tag="VI615_RS26605"
                     /old_locus_tag="VI615_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072013440.1"
                     /translation="MHEPPVQSDCCALSGNYHLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            6604..6795
                     /locus_tag="VI615_RS26610"
                     /old_locus_tag="VI615_26610"
     CDS             6604..6795
                     /locus_tag="VI615_RS26610"
                     /old_locus_tag="VI615_26610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044525164.1"
                     /translation="MSKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            6795..7196
                     /locus_tag="VI615_RS26615"
                     /old_locus_tag="VI615_26615"
     CDS             6795..7196
                     /locus_tag="VI615_RS26615"
                     /old_locus_tag="VI615_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7981..8056)
                     /locus_tag="VI615_RS26620"
                     /old_locus_tag="VI615_26620"
     tRNA            complement(7981..8056)
                     /locus_tag="VI615_RS26620"
                     /old_locus_tag="VI615_26620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8021..8023),aa:Arg,seq:tct)
                     /name="tRNA-Arg"
     gene            complement(8237..8479)
                     /locus_tag="VI615_RS26625"
                     /old_locus_tag="VI615_26625"
     CDS             complement(8237..8479)
                     /locus_tag="VI615_RS26625"
                     /old_locus_tag="VI615_26625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017446859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_074196216.1"
                     /translation="MSKESELEFRTKELERQMKGVQRRIEVVNAKYDSQTKKQERRIRD
                     LEIKTAVQSGVTQREVANIYELSPGRVNQIIKKVG"
                     /name="hypothetical protein"
     gene            8869..9264
                     /locus_tag="VI615_RS26630"
                     /old_locus_tag="VI615_26630"
     CDS             8869..9264
                     /locus_tag="VI615_RS26630"
                     /old_locus_tag="VI615_26630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_126066683.1"
                     /translation="MNANEVKDILEHSNHDDWIVDDETGSFTYKNDLNLHIKRADYDNY
                     NSFSEPWAVSHPNPNACSVQYTVNYGSSFVDRKTLVSVDGHRATLPMPASANDLTVTSA
                     DVNFAKIVDTGERVDEYILRSGLKVQP"
                     /name="hypothetical protein"
     Typing          complement(1421..1828)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6076..6483)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.355"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gtgcttaagg gacgttatgg tgactttttt taatgtcact gacctaaact tcaaaattgt
       61 atttggttga gtttaaaatg gaatctatca ctattagtat agagtcgaaa taaaaattct
      121 ttattctctt tggatgccag tatcttccat gagcaagcca atgcctgaat ttaaaacatg
      181 tttttagatc gttgtagtct ttttttaatc tagcatcatc ttcattcatt ctggtattta
      241 ttaaatcatc atctaatttt attttttctc cttttgtttt atcatatatc tcttttattt
      301 ttcttgataa tgggtctctt tttttattgc agtacttctg atctgcatct aatcgaaata
      361 ttgcttcaat agctgttaac aataaaaggc aatgtgttgt gtcattttct tttagtatta
      421 agtctttttc tctttcaatc tccggctcac ttaagccgac gaaatcggca tggtaatctg
      481 ggttctttgt tgaataccgc agaattacag cacgatcgag tgctatcttt ttatgtacga
      541 catagtcaat gtctatgtgt agaccagatc ttttaattgc atgaatccta gccatttatc
      601 attctcataa taatattcgt aatggtctcg gaattcacat catcaaattt aacccatcga
      661 ctatcgctag gcattgtttt ccattcccaa tcatcaattg acggcttttt cacatcttca
      721 tcggtcttat ctttttctgg tttatatgcc ccgactacgt taactcttac ttttattaaa
      781 tcgctggggc ttttgactcc ttttctgatc agaatgaatt tttcttttcc aaatggccct
      841 gtgacgtcaa cccttccttt tgaacctatt agcatggttc ctactggctc aaattttatt
      901 ttacttgagt ttatagtgag tattagagag tcgatttcat aatcacctag agactcttca
      961 tttattgata tttttttctt gattgtttta tcaatccatc tttgattagt tcgtcaatag
     1021 attctaatgt aatagtatat aatttttcaa caaggcttat gtattcttct atttgttccg
     1081 ctgggtcgaa tttttgttct tcttcttctt ttttttgttt ttcaattata tctttcagcg
     1141 ttttcatgtt cattttttgt aacctctaga tcggaattat tttacgattc tagcctttgc
     1201 tttggtgtcg agcaatgtgt tgttcttgac gcttaacttg ctcggaggta ttgtcgcttc
     1261 tgaaacgaaa aaaccgcctt ggcgggcggt tttttcgaag gttcatagag ttggcgctcg
     1321 ttgaaccgag gtaactggct tggaggagcg cagtcaccaa aacttgttct ttcagtttag
     1381 ccttaactgg cgcataactt caagacaaac tcctctaaat cagttaccag tggctgctgc
     1441 cagtggcgct tttgcatgtc ttaccgggtt ggactcaaga tgatagttac cggataaggc
     1501 gcagcagtcg gactgaacgg ggggttcgtg catacagtcc agcttggagc gaactgccta
     1561 cccggaactg agtgtcaggc gtggaatgag ataaacgcgg ccataacagc ggattgacac
     1621 tggtaaaccg aaaggcagga acaggagagc gcacgaggga gccatcaggg ggaaacgcct
     1681 ggtatcttta tagtcctgtc gggtttcgcc accactgatt tgagcgtcaa attctgtgat
     1741 gcttgtcagg ggggcggagc ctatggaaaa acggccgccg tgcggcattt ccttttcccc
     1801 gtcacgctac cgctcggttt tctcgagctc ttgtacgcat actcctgccg ctcgccgcag
     1861 ccgaacggca ggagcgtagc gactgagtga gcgaggaagc ggaaaagaga acatacctac
     1921 actgagcact gacgctctga ggcctcttat gtctaaacaa cagaaaacag cgctgaacat
     1981 ggctaaattc atccagaatc agtctctgct cctgctggag aaacttaacg agcttgatct
     2041 cgatgtcgaa gccgaccttt gtgagaaact ccacgatgat gcggagcatc ttttccgtac
     2101 actgtcctcg agactggatg cattacagga tggtaactga tggccagagt gtctattagt
     2161 gaggctgccc gactgacggg taagagtaga acaacactgc acaggttaat taaagcaggt
     2221 gacctgtcca cctgtagcgg tgaacgtaat gcaaaaatgg tagatacttc agagctttta
     2281 cgtgtctttg gtccctttga gcaaccgaag ggtgaacacc attctggaca ggtctctgga
     2341 cagcgtgata cagcctctgc tgaccagagt gaacaggtga ttcgtcatct gaaacaggag
     2401 attgaacacc tgaacacctt acttttgtcc aaggattcgc acattgacag cctgaaacag
     2461 gccatgctcc ttcttgaaaa tcaccaggca aaattaactg aatcaccggc gccatggtgg
     2521 aaattctgga aaaaatcctg accccgtcat gcttcagttt tcctctccgg ccatcccggg
     2581 gagttcctga accttcccaa aaatcgtcag atctctgaac gctcctcacc actaaaaata
     2641 cccacacccc acgcaaaatt aaaattttga agataactca ttaatatcag ttagttgtgg
     2701 gttattaaat tacgcagtat tactttctgt aaggtgaatc caagtcgtcg gcgattcgtg
     2761 tacgacttgc cgacgactcg tcatagagtc atcgacgact cacataagac tatcagtaac
     2821 ttatgacgac ttgactctag agtcgaatcg tcattttggt gtttttttga ccttgacgac
     2881 tctagagtca agtcacgagt cgaatcgccg gtgaatcgtg tggtgagtcg tccgtcgact
     2941 tgccataacg tgattgccgg ccgtcgacga acccgattcc tgatcgtgct gaaaaccggt
     3001 tttccggctg cgccggcaac gcttcacggc cacgcaataa attgcgtccc cgttcaccgt
     3061 tttcggatgg gaagaacgcc ggaaacagga cttaccctgg ctgaatggcg tgaattttcg
     3121 cgctatgcac ttgcatgcat actcatgcat gccgtaaaaa cagagcctgc gcgtttctgg
     3181 cgggttttcg ggtggtttgt tgcctgtttt accggtttcc cgtcagaaac gccctgaggc
     3241 cgttttagcg gtgcgcgtaa tgcgacgtta tggtaaatgc tgtggccaga atagttgcct
     3301 cccccctttt agggggggag gcttttggtg gagacaatgg gatttgaacc cacaaccatc
     3361 agattagaag tccgctgctc taccgttgaa gctatatccc catctggacc caacaaagtc
     3421 gggaccgtct ttgatttaac atcactgcat gctttttgtg aagtttttta tgtgtttacc
     3481 ggtcgcatca gaacagggta tgtaatgaga ccttatggta aaaagcacgt cagtttgtat
     3541 tgcgagaaaa aaccgccttt tggcggtttt ttctctagag attagccgac ttttttaatg
     3601 atctgattaa cgcgacccgg cgacagttcg taaatgtttg caacctcacg ttgcgtaacg
     3661 cctgactgga ctgctgtttt gatttccaaa tcacgaatgc gacgttcttg cttcttggtt
     3721 tggctgtcgt atttggcgtt aaccacctca atacgtcgct ggacaccttt catttgacgt
     3781 tcgagttctt ttgttctgaa ctctagttct gattctttac tcatatgata tactcgctaa
     3841 gtaaggttat tgttgggtgt cattcgaagg tttcttcttg ccggaaggtc ttaacctatg
     3901 tttgacaaat caacaatagc ctttttttac gatcacagca acttctataa tcagtgtttc
     3961 cccttatttt tctttgtttt tattttccaa taaaaacaat aagttgcgat tggtttaatt
     4021 ctgatattaa aaaggctaaa cacacctatt ttatgtatat gatttttaag tgaaaaaaca
     4081 aatcgatgtt tagtatttat tatttgattc tttactggaa aatttgattg tacatagaga
     4141 acttttcgga gcagtgtggt gtagaactca atcgatcttt acattctaac cattgattta
     4201 tatgggggta tgcatgaatg ctaatgaagt aaaagacatt cttgaacatt ctaatcatga
     4261 tgattggatt gtggatgatg agactgggtc gtttacatat aaaaacgact taaatttgca
     4321 cattaagaga gcggactatg acaactacaa ctcctttagt gaaccctggg ctgttagtca
     4381 tccgaatccg aatgcctgtt cagtacagta caccgtaaac tatggttcat catttgttga
     4441 tagaaagact ttggtttctg tcgatggtca ccgcgcaacc ttgcctatgc ctgcatccgc
     4501 aaatgattta accgtgacat cagctgacgt gaactttgcc aaaattgtag atactgggga
     4561 gcgtgttgat gaatacattc tccgctctgg tcttaaagtt caaccatgat ttaaatgtga
     4621 gccagcaatc taaaattgct ggctcataat gtgctgtgct taagggacgt tatggtgact
     4681 ttttttaatg tcactgacct aaacttcaaa attgtatttg gttgagttta aaatggaatc
     4741 tatcactatt agtatagagt cgaaataaaa attctttatt ctctttggat gccagtatct
     4801 tccatgagca agccaatgcc tgaatttaaa acatgttttt agatcgttgt agtctttttt
     4861 taatctagca tcatcttcat tcattctggt atttattaaa tcatcatcta attttatttt
     4921 ttctcctttt gttttatcat atatctcttt tatttttctt gataatgggt ctcttttttt
     4981 attgcagtac ttctgatctg catctaatcg aaatattgct tcaatagctg ttaacaataa
     5041 aaggcaatgt gttgtgtcat tttcttttag tattaagtct ttttctcttt caatctccgg
     5101 ctcacttaag ccgacgaaat cggcatggta atctgggttc tttgttgaat accgcagaat
     5161 tacagcacga tcgagtgcta tctttttatg tacgacatag tcaatgtcta tgtgtagacc
     5221 agatctttta attgcatgaa tcctagccat ttatcattct cataataata ttcgtaatgg
     5281 tctcggaatt cacatcatca aatttaaccc atcgactatc gctaggcatt gttttccatt
     5341 cccaatcatc aattgacggc tttttcacat cttcatcggt cttatctttt tctggtttat
     5401 atgccccgac tacgttaact cttactttta ttaaatcgct ggggcttttg actccttttc
     5461 tgatcagaat gaatttttct tttccaaatg gccctgtgac gtcaaccctt ccttttgaac
     5521 ctattagcat ggttcctact ggctcaaatt ttattttact tgagtttata gtgagtatta
     5581 gagagtcgat ttcataatca cctagagact cttcatttat tgatattttt ttcttgattg
     5641 ttttatcaat ccatctttga ttagttcgtc aatagattct aatgtaatag tatataattt
     5701 ttcaacaagg cttatgtatt cttctatttg ttccgctggg tcgaattttt gttcttcttc
     5761 ttcttttttt tgtttttcaa ttatatcttt cagcgttttc atgttcattt tttgtaacct
     5821 ctagatcgga attattttac gattctagcc tttgctttgg tgtcgagcaa tgtgttgttc
     5881 ttgacgctta acttgctcgg aggtattgtc gcttctgaaa cgaaaaaacc gccttggcgg
     5941 gcggtttttt cgaaggttca tagagttggc gctcgttgaa ccgaggtaac tggcttggag
     6001 gagcgcagtc accaaaactt gttctttcag tttagcctta actggcgcat aacttcaaga
     6061 caaactcctc taaatcagtt accagtggct gctgccagtg gcgcttttgc atgtcttacc
     6121 gggttggact caagatgata gttaccggat aaggcgcagc agtcggactg aacggggggt
     6181 tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt caggcgtgga
     6241 atgagataaa cgcggccata acagcggatt gacactggta aaccgaaagg caggaacagg
     6301 agagcgcacg agggagccat cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6361 tcgccaccac tgatttgagc gtcaaattct gtgatgcttg tcaggggggc ggagcctatg
     6421 gaaaaacggc cgccgtgcgg catttccttt tccccgtcac gctaccgctc ggttttctcg
     6481 agctcttgta cgcatactcc tgccgctcgc cgcagccgaa cggcaggagc gtagcgactg
     6541 agtgagcgag gaagcggaaa agagaacata cctacactga gcactgacgc tctgaggcct
     6601 cttatgtcta aacaacagaa aacagcgctg aacatggcta aattcatcca gaatcagtct
     6661 ctgctcctgc tggagaaact taacgagctt gatctcgatg tcgaagccga cctttgtgag
     6721 aaactccacg atgatgcgga gcatcttttc cgtacactgt cctcgagact ggatgcatta
     6781 caggatggta actgatggcc agagtgtcta ttagtgaggc tgcccgactg acgggtaaga
     6841 gtagaacaac actgcacagg ttaattaaag caggtgacct gtccacctgt agcggtgaac
     6901 gtaatgcaaa aatggtagat acttcagagc ttttacgtgt ctttggtccc tttgagcaac
     6961 cgaagggtga acaccattct ggacaggtct ctggacagcg tgatacagcc tctgctgacc
     7021 agagtgaaca ggtgattcgt catctgaaac aggagattga acacctgaac accttacttt
     7081 tgtccaagga ttcgcacatt gacagcctga aacaggccat gctccttctt gaaaatcacc
     7141 aggcaaaatt aactgaatca ccggcgccat ggtggaaatt ctggaaaaaa tcctgacccc
     7201 gtcatgcttc agttttcctc tccggccatc ccggggagtt cctgaacctt cccaaaaatc
     7261 gtcagatctc tgaacgctcc tcaccactaa aaatacccac accccacgca aaattaaaat
     7321 tttgaagata actcattaat atcagttagt tgtgggttat taaattacgc agtattactt
     7381 tctgtaaggt gaatccaagt cgtcggcgat tcgtgtacga cttgccgacg actcgtcata
     7441 gagtcatcga cgactcacat aagactatca gtaacttatg acgacttgac tctagagtcg
     7501 aatcgtcatt ttggtgtttt tttgaccttg acgactctag agtcaagtca cgagtcgaat
     7561 cgccggtgaa tcgtgtggtg agtcgtccgt cgacttgcca taacgtgatt gccggccgtc
     7621 gacgaacccg attcctgatc gtgctgaaaa ccggttttcc ggctgcgccg gcaacgcttc
     7681 acggccacgc aataaattgc gtccccgttc accgttttcg gatgggaaga acgccggaaa
     7741 caggacttac cctggctgaa tggcgtgaat tttcgcgcta tgcacttgca tgcatactca
     7801 tgcatgccgt aaaaacagag cctgcgcgtt tctggcgggt tttcgggtgg tttgttgcct
     7861 gttttaccgg tttcccgtca gaaacgccct gaggccgttt tagcggtgcg cgtaatgcga
     7921 cgttatggta aatgctgtgg ccagaatagt tgcctccccc cttttagggg gggaggcttt
     7981 tggtggagac aatgggattt gaacccacaa ccatcagatt agaagtccgc tgctctaccg
     8041 ttgaagctat atccccatct ggacccaaca aagtcgggac cgtctttgat ttaacatcac
     8101 tgcatgcttt ttgtgaagtt ttttatgtgt ttaccggtcg catcagaaca gggtatgtaa
     8161 tgagacctta tggtaaaaag cacgtcagtt tgtattgcga gaaaaaaccg ccttttggcg
     8221 gttttttctc tagagattag ccgacttttt taatgatctg attaacgcga cccggcgaca
     8281 gttcgtaaat gtttgcaacc tcacgttgcg taacgcctga ctggactgct gttttgattt
     8341 ccaaatcacg aatgcgacgt tcttgcttct tggtttggct gtcgtatttg gcgttaacca
     8401 cctcaatacg tcgctggaca cctttcattt gacgttcgag ttcttttgtt ctgaactcta
     8461 gttctgattc tttactcata tgatatactc gctaagtaag gttattgttg ggtgtcattc
     8521 gaaggtttct tcttgccgga aggtcttaac ctatgtttga caaatcaaca atagcctttt
     8581 tttacgatca cagcaacttc tataatcagt gtttcccctt atttttcttt gtttttattt
     8641 tccaataaaa acaataagtt gcgattggtt taattctgat attaaaaagg ctaaacacac
     8701 ctattttatg tatatgattt ttaagtgaaa aaacaaatcg atgtttagta tttattattt
     8761 gattctttac tggaaaattt gattgtacat agagaacttt tcggagcagt gtggtgtaga
     8821 actcaatcga tctttacatt ctaaccattg atttatatgg gggtatgcat gaatgctaat
     8881 gaagtaaaag acattcttga acattctaat catgatgatt ggattgtgga tgatgagact
     8941 gggtcgttta catataaaaa cgacttaaat ttgcacatta agagagcgga ctatgacaac
     9001 tacaactcct ttagtgaacc ctgggctgtt agtcatccga atccgaatgc ctgttcagta
     9061 cagtacaccg taaactatgg ttcatcattt gttgatagaa agactttggt ttctgtcgat
     9121 ggtcaccgcg caaccttgcc tatgcctgca tccgcaaatg atttaaccgt gacatcagct
     9181 gacgtgaact ttgccaaaat tgtagatact ggggagcgtg ttgatgaata cattctccgc
     9241 tctggtctta aagttcaacc atgatttaaa tgtgagccag caatctaaaa ttgctggctc
     9301 ataatgtgct
//
