LOCUS       NZ_CP141573            12283 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP35_L8_42.22_ST2096_OXA232 plasmid
            pK35_OXA232, complete sequence.
ACCESSION   NZ_CP141573
VERSION     NZ_CP141573.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38771710
            Assembly: GCF_035598475.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12283)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Assembly Name                     :: rrp8_bc10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 11:39:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,344
            CDSs (total)                      :: 5,222
            Genes (coding)                    :: 5,024
            CDSs (with protein)               :: 5,024
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 118 of 198
            Pseudo Genes (incomplete)         :: 76 of 198
            Pseudo Genes (internal stop)      :: 26 of 198
            Pseudo Genes (multiple problems)  :: 20 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141573.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP35_L8_42.22_ST2096_OXA232"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pK35_OXA232"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-09-03"
                     /genotype="ST2096"
     gene            join(<12249..12283,1..118)
                     /locus_tag="VI604_RS00600"
                     /old_locus_tag="VI604_00600"
                     /pseudo=""
     CDS             join(<12249..12283,1..118)
                     /locus_tag="VI604_RS00600"
                     /old_locus_tag="VI604_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(525..1322)
                     /locus_tag="VI604_RS00605"
                     /old_locus_tag="VI604_00605"
     CDS             complement(525..1322)
                     /locus_tag="VI604_RS00605"
                     /old_locus_tag="VI604_00605"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            1666..1947
                     /locus_tag="VI604_RS00610"
                     /old_locus_tag="VI604_00610"
     CDS             1666..1947
                     /locus_tag="VI604_RS00610"
                     /old_locus_tag="VI604_00610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(2229..3683)
                     /locus_tag="VI604_RS00615"
                     /old_locus_tag="VI604_00615"
     CDS             complement(2229..3683)
                     /locus_tag="VI604_RS00615"
                     /old_locus_tag="VI604_00615"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3664..4002)
                     /locus_tag="VI604_RS00620"
                     /old_locus_tag="VI604_00620"
     CDS             complement(3664..4002)
                     /locus_tag="VI604_RS00620"
                     /old_locus_tag="VI604_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(3984..4376)
                     /locus_tag="VI604_RS00625"
                     /old_locus_tag="VI604_00625"
     CDS             complement(3984..4376)
                     /locus_tag="VI604_RS00625"
                     /old_locus_tag="VI604_00625"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(4784..5731)
                     /locus_tag="VI604_RS00630"
                     /old_locus_tag="VI604_00630"
     CDS             complement(4784..5731)
                     /locus_tag="VI604_RS00630"
                     /old_locus_tag="VI604_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            <6107..6259
                     /locus_tag="VI604_RS00635"
                     /old_locus_tag="VI604_00635"
                     /pseudo=""
     CDS             <6107..6259
                     /locus_tag="VI604_RS00635"
                     /old_locus_tag="VI604_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(6666..7463)
                     /locus_tag="VI604_RS00640"
                     /old_locus_tag="VI604_00640"
     CDS             complement(6666..7463)
                     /locus_tag="VI604_RS00640"
                     /old_locus_tag="VI604_00640"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            7807..8088
                     /locus_tag="VI604_RS00645"
                     /old_locus_tag="VI604_00645"
     CDS             7807..8088
                     /locus_tag="VI604_RS00645"
                     /old_locus_tag="VI604_00645"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(8370..9824)
                     /locus_tag="VI604_RS00650"
                     /old_locus_tag="VI604_00650"
     CDS             complement(8370..9824)
                     /locus_tag="VI604_RS00650"
                     /old_locus_tag="VI604_00650"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9805..10143)
                     /locus_tag="VI604_RS00655"
                     /old_locus_tag="VI604_00655"
     CDS             complement(9805..10143)
                     /locus_tag="VI604_RS00655"
                     /old_locus_tag="VI604_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(10125..10517)
                     /locus_tag="VI604_RS00660"
                     /old_locus_tag="VI604_00660"
     CDS             complement(10125..10517)
                     /locus_tag="VI604_RS00660"
                     /old_locus_tag="VI604_00660"
                     /inference="COORDINATES: protein motif:HMM:NF037437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(10926..11873)
                     /locus_tag="VI604_RS00665"
                     /old_locus_tag="VI604_00665"
     CDS             complement(10926..11873)
                     /locus_tag="VI604_RS00665"
                     /old_locus_tag="VI604_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     ARG             complement(529..1322)
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(6670..7463)
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(4798..5719)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(10940..11861)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(2233..3683)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8374..9824)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 catgaaagca aagcgaatgc ggtcccaaag cgcctctgta gaaacgaatt tgagcgaggc
       61 atttgatgcc atcgtctgcg tcccaagcgc cggcaaggac ggcctggttg acctatagta
      121 aaccggtaga tgaaaatgat ggagaaccaa tatcagcgcc ttcttggact ttttcgtcaa
      181 acgctttagc agccaagaaa gcctacaatc cttgatgggc taggcacttt atcgcagcgg
      241 cattcacctt tactgacatc gccactcctt tattcacacc cccatgttga gtgaccatgg
      301 ccattgagat aacaaaaaag cctagcaaat tggctaggct ttttacgttt aggaatgtga
      361 tgctaatgct caggctgaaa gttaacgcgc ggagcttaag tcgattaggc ctgaccgatt
      421 aagtatgagc cattaactac aagcgcatcg agcatcagca ttttgtccat atatctttac
      481 agcttatatg acggctattt cacacaaata cgcgctaacc acttctaggg aataattttc
      541 tcctgtttga gcacttcttt tgtgatggct tggcgcagcc ctaaaccatc cgatgtgggc
      601 atatccatat tcatcgcaaa aaaccacaca ttatcatcaa gttcaaccca accaacccac
      661 cagccaatct taggttcgat actagtcgag tatcccgttt tagcccgaat aatatagtcg
      721 ccattggctt cggtcagcat ggcttgtttc acgatgcgct gactacgctc agaaacgtgc
      781 agcttgttgt gatacagctt gcgtaaaaaa gcgatttgct gggtagccga aatgcgaata
      841 ccaccatcga gccaaaaact gtctacattg cccgagatat cctcattgcc ataatcgaag
      901 gcgtgcagca ttttactcat acgtgcctca ccaatttggc gggcaaattc ttgataaaca
      961 ggcacaactg agtacttcat cgcggtaatt aagtcatggt cacgattcca agcggcgata
     1021 tcacgcgtct gtccatccca cttaaagact tggtgttcat ccttaaccac gcccaaatcg
     1081 agggcgatca agctattggg aattttaaag gtagatgcgg gtaaaaatgc ttggttcgcc
     1141 cgtttaagat tattggtaaa tccttgctgc ttattctcat tccagagcac aactacgccc
     1201 tgtgatttat gttcagtaaa gtgagcattc caacttttgt tttcttgcca ttcctttgct
     1261 accgctggca ttccgataat cgatgccacc aaaaacacag ccgataaggc taatacacgc
     1321 ataacgtccc cttgcttaat gcaatatatc catacaaatt aagtgctaag cttatcattc
     1381 tctgccctct cattccctag caaactgctg accaattgtg gcaacaatca ggcacaagct
     1441 ttaactatta cctaaattcc acgtgtgttt tttattagct tcaaaaatca ctatttcacg
     1501 aagaatttag actgcttctc acacattgta acattattta caaccacctt tcaatcattt
     1561 ttgataaatc attgatttca tctttgctgc aatgatactt aataaactct gcaagttatc
     1621 cacagagcaa cactcaattt tattgatgat attcttaggg ggagtatggg attttttaac
     1681 ctatctaaag cgcttgagga ggtgtctgat gcttttgggg ctaaagaaac cgcaatcgct
     1741 agtgccaagt tgttaggtaa gggagttgtt aacctagctc attttgcagc aacaacaggt
     1801 ttggattcgg tggtcaaaca aacgagcaac aaagttctca acagcgagtc ttcaaccgaa
     1861 gaacaacgag aacaggctaa aaagtctgcc gcatgggcag acgcgagaat cgccgctaga
     1921 aataaagcag aaaacaccga agactagctg aacgcacatt tcgacaatga tgtggactac
     1981 atcgttgatc ataacttcac ggtgcgctag cttccataga agccaactaa taacaaggag
     2041 ttagcatgtc tgattcagat ttcaaaaata gccctgagta tgatgactgg attgaatctg
     2101 gtggtagaga tgaagattac agttactact atgaaaaatg gcatagaaga actcaacgat
     2161 aattcccgcc gataaaagga aagtgatagc actttccttt ttttataact gacggccgtt
     2221 ttgttccgtc aacgctcaaa gcctcgcgat ctgtctccac gctcccagcc tcgatccagt
     2281 gccttcagga gcctgtgttc cctttctttt gcaacttgct gactagcccg gtcaagccct
     2341 ccagctgctt gctcaagccg tccacttgct gcgttaagcc gttcacctgc ctcgtcaatc
     2401 gctctgctgc tgccctgctg atctcggcct cttgtttggt gctctcgtac atgctcgcca
     2461 agttcgcgta gctttcctgc catgccttgt gctgctgctc gtatgcgctc tgcaaagcct
     2521 cgaacgcgca gagcaattcc cgctccaatc ccgtcattta gtaccccttc ggcacgatcc
     2581 aataggtatc cttcccccaa acggttggat tggggtcgga gatctgcacg caaaggtgct
     2641 ccaatttctt cccgcactgg gtcagcttta gcttgccgcc cttctctgcc aaggcttgga
     2701 gagtggcttg ccgctccgct atcaattccc cctgatacca gaggatcccg ctcgttgcgc
     2761 tcaagaacag gacgatgctg accagcatcc agatccacgt tttgagcaac agccagccca
     2821 ttttctggct gtgctcggca atagcggcgc tcattttctc ctcgcttgat ttcaacgccc
     2881 cggagatagc tttctcgtgc ctcgtgaatt cttcggtcag acgcttctcg atagcgttgg
     2941 cctgctgctt cgagctctgc tcgaagtcct tcgccatgtc taaaatcttg ctcatacagc
     3001 acccccttta atcggatgtt ccgccccccg tccgggtcgg caatgctcag gctgtttttg
     3061 gtttcgcgta ccacgctgaa acccgcaccc tgcaaggctt tgcgcacatc atcgcggctc
     3121 tggatctccc ctgctccggc catgcgtaaa agcccgtctg taatggcctg agcggcctcc
     3181 ttgtgccccc tcggcaaatc ctttggcgtg ataacggcct gacggtttaa cggatcgtcc
     3241 gggtcgtgca atttcagctc cccgtttacc acggttcgcc acgcatcgag gcgcggattg
     3301 tcggccttgt ggaagtaggg ttgcagccgc ttcccgctct gcaattcgat gttcgggact
     3361 acaaaattca gctccagccg ccccttgtcg cggtgctcta cccacaaaac agcgtactgg
     3421 ttgccctcca gtccgggcag gagcgccttt tcaaagtcgc tcatcagctt gtccttggtc
     3481 gccctgggta aatcagcctc ggcaaacgac agaacgccag aggtgtattt tttggcgtag
     3541 ggactggaat caatcagggc ttcaatcacc gcagggttgc cccggtcgag cgttgcgcct
     3601 tcccggttcc cgtcacggcc caacaagtag ccaacaggcc cggagccccg gcctacccca
     3661 cggttatgga atttgatgat catggtgcag ttcctccagc tcgcggccta tagcggacag
     3721 ctccgctatg atgcgcagcc gatccaacgg cccccactcc ccggaattaa cgcgccgggc
     3781 gatctggttc aggttgttgc ccattccggc caactgccgc aagagcgccg gatctacggt
     3841 aggcgccggc gttcggcgct ggggttgcgc aacaccgaga cattgctccc gcatccactc
     3901 cgccagttgg gccttggggc agagctcccg caggcgctca agctccgcat ctgagacccg
     3961 gatcttgata gagcggaggc gctttaacgg ctggccagcc atgcagaggc ctccgctaag
     4021 gcgtcctcat agaggctatc gagtagcgtc tggagcgcct tttcatcccg aaaatcgcag
     4081 tgctccggca aggggaaaac acccccgccg acctcttcgc cgttctccag caaacgcaga
     4141 ttccagcccc cgccaagacc gagagtgcgg ggggtgattt cgtagtcgta ttccgttgtt
     4201 gtgggttcgt cacttaccat agctaacacc tcatcggtgg aaatttttcc attgatataa
     4261 tcttcaagta gaattttgaa ttcatcggtt atggcctccc cctctagttg gaaactagaa
     4321 ataacgccat tgactgcctt ccttctcgaa tctttctcta tctgtgttgt tgacataggg
     4381 cctcgtccat tctccagagc caagaagcca ccgcgcagac cgtcccgaag gggcggatcg
     4441 agcgggggcc acagggggtt tcgggggggc gtagcacccc tgaacaaggc cacagaggcc
     4501 cgagaccgcg aagcaggtag aggggcctct gtgtagcctt gcttagtaca ttattcaacc
     4561 atgggcggga tactgcaagc cctagagcaa gatcaaggga aagagcggct ccgtggataa
     4621 ctgctgccgc ccacaccccc cgaaaaaaca ggggggcgat ggacggacag gcgcagttgt
     4681 ccacctcgcc aaagatccgc gctaaaagga ttttttaagg ctaaaagaca agagcgcagg
     4741 aaccccccta aagggggcta ttatctgata aggcttgttt cgatcacgga cgatcacgct
     4801 ttaggtaaac gctcaccgta ctgggggaga tttgaagatc gtccgctata tcgcggttgg
     4861 tgtacccctg agccttaagc cgcagaacct ccggcaatag atcggacttg tccttaccgc
     4921 tattgctcgg ccttccccct cccttggata ccttgccgcc cttcgcacct ctccgggcct
     4981 gctcctctct gaaccctgcg gggcttaggt gctggtgcgt ccatttggca atggacttgg
     5041 ccgtgtgccg gacttcgttt gcggggagcg gctgctcgaa ctgggcgtta tatcctgatg
     5101 cccggtcaac acaagcggcc aaccattcgg ggtaatccgg ccagccctgc cggatcgctt
     5161 tataggccca caggcgcgtt ttctcgaaca gggtacagtt tcgcccaagc ccgtactcag
     5221 gaagcgattt gcggccattg taggagctca gatcgaggta atcggatagc caggctaaat
     5281 cgtagagccg gggttcccat acctgcacca gccagtgctc atgcaggggg ttcttgcaga
     5341 tcagcccgga gtagcccata tccgccccta atttctcccg gagcgcggcc tcgatggcgg
     5401 cggcataccg cagaggggct gcatttccat ccggggcagt gcggatcggc gtatccagtc
     5461 cgtagatcag atgcgcgtgg ccgttggcct tgttcgtgac ggtaatgtta ggggctggag
     5521 cgcctacgtc atcccaagat aacgtagcgg ctgcatggtc tacgtcaaaa acgatccagt
     5581 ggcggtgcgt tggcccgttc ggctggatgt aacgcgcacc aagggcgcgg gcaacatccg
     5641 caatccgtag gcccgttgta agctcgtcgg aatagtaggg tttccggggc aggcgctccc
     5701 gaaacagctc aagctgtgcg cactttctca agcaccaccg cgcttattgg cggcgtgggg
     5761 ttgtttggca tgatccatgt gatagacttc tcttgtgatt cgtcacggcc cccgtttttc
     5821 gcagctccgc caagatgcaa gcgataacgg ggggttgtct tttctggaca atacgttaca
     5881 gcaggcacat cctagagagc aacgttagtt gcatgtaacc tttcaccctg gaacatattt
     5941 ccacctcctt ctgcttctgc gctgttttga attgcttgat ctacgcctct aactaggtcg
     6001 atccttggga ttgagtggca ccattgtcag tgcaagaata ctgatccata taagtctctc
     6061 tagtcggaca acaccaagtg ttgaagagat caaaaatcaa cctatatcgg aggattcatg
     6121 tctgacattg acagatgccc ccatgaaagc aaagcgaatg cggtcccaaa gcgcctctgt
     6181 agaaacgaat ttgagcgagg catttgatgc catcgtctgc gtcccaagcg ccggcaagga
     6241 cggcctggtt gacctatagt aaaccggtag atgaaaatga tggagaacca atatcagcgc
     6301 cttcttggac tttttcgtca aacgctttag cagccaagaa agcctacaat ccttgatggg
     6361 ctaggcactt tatcgcagcg gcattcacct ttactgacat cgccactcct ttattcacac
     6421 ccccatgttg agtgaccatg gccattgaga taacaaaaaa gcctagcaaa ttggctaggc
     6481 tttttacgtt taggaatgtg atgctaatgc tcaggctgaa agttaacgcg cggagcttaa
     6541 gtcgattagg cctgaccgat taagtatgag ccattaacta caagcgcatc gagcatcagc
     6601 attttgtcca tatatcttta cagcttatat gacggctatt tcacacaaat acgcgctaac
     6661 cacttctagg gaataatttt ctcctgtttg agcacttctt ttgtgatggc ttggcgcagc
     6721 cctaaaccat ccgatgtggg catatccata ttcatcgcaa aaaaccacac attatcatca
     6781 agttcaaccc aaccaaccca ccagccaatc ttaggttcga tactagtcga gtatcccgtt
     6841 ttagcccgaa taatatagtc gccattggct tcggtcagca tggcttgttt cacgatgcgc
     6901 tgactacgct cagaaacgtg cagcttgttg tgatacagct tgcgtaaaaa agcgatttgc
     6961 tgggtagccg aaatgcgaat accaccatcg agccaaaaac tgtctacatt gcccgagata
     7021 tcctcattgc cataatcgaa ggcgtgcagc attttactca tacgtgcctc accaatttgg
     7081 cgggcaaatt cttgataaac aggcacaact gagtacttca tcgcggtaat taagtcatgg
     7141 tcacgattcc aagcggcgat atcacgcgtc tgtccatccc acttaaagac ttggtgttca
     7201 tccttaacca cgcccaaatc gagggcgatc aagctattgg gaattttaaa ggtagatgcg
     7261 ggtaaaaatg cttggttcgc ccgtttaaga ttattggtaa atccttgctg cttattctca
     7321 ttccagagca caactacgcc ctgtgattta tgttcagtaa agtgagcatt ccaacttttg
     7381 ttttcttgcc attcctttgc taccgctggc attccgataa tcgatgccac caaaaacaca
     7441 gccgataagg ctaatacacg cataacgtcc ccttgcttaa tgcaatatat ccatacaaat
     7501 taagtgctaa gcttatcatt ctctgccctc tcattcccta gcaaactgct gaccaattgt
     7561 ggcaacaatc aggcacaagc tttaactatt acctaaattc cacgtgtgtt ttttattagc
     7621 ttcaaaaatc actatttcac gaagaattta gactgcttct cacacattgt aacattattt
     7681 acaaccacct ttcaatcatt tttgataaat cattgatttc atctttgctg caatgatact
     7741 taataaactc tgcaagttat ccacagagca acactcaatt ttattgatga tattcttagg
     7801 gggagtatgg gattttttaa cctatctaaa gcgcttgagg aggtgtctga tgcttttggg
     7861 gctaaagaaa ccgcaatcgc tagtgccaag ttgttaggta agggagttgt taacctagct
     7921 cattttgcag caacaacagg tttggattcg gtggtcaaac aaacgagcaa caaagttctc
     7981 aacagcgagt cttcaaccga agaacaacga gaacaggcta aaaagtctgc cgcatgggca
     8041 gacgcgagaa tcgccgctag aaataaagca gaaaacaccg aagactagct gaacgcacat
     8101 ttcgacaatg atgtggacta catcgttgat cataacttca cggtgcgcta gcttccatag
     8161 aagccaacta ataacaagga gttagcatgt ctgattcaga tttcaaaaat agccctgagt
     8221 atgatgactg gattgaatct ggtggtagag atgaagatta cagttactac tatgaaaaat
     8281 ggcatagaag aactcaacga taattcccgc cgataaaagg aaagtgatag cactttcctt
     8341 tttttataac tgacggccgt tttgttccgt caacgctcaa agcctcgcga tctgtctcca
     8401 cgctcccagc ctcgatccag tgccttcagg agcctgtgtt ccctttcttt tgcaacttgc
     8461 tgactagccc ggtcaagccc tccagctgct tgctcaagcc gtccacttgc tgcgttaagc
     8521 cgttcacctg cctcgtcaat cgctctgctg ctgccctgct gatctcggcc tcttgtttgg
     8581 tgctctcgta catgctcgcc aagttcgcgt agctttcctg ccatgccttg tgctgctgct
     8641 cgtatgcgct ctgcaaagcc tcgaacgcgc agagcaattc ccgctccaat cccgtcattt
     8701 agtacccctt cggcacgatc caataggtat ccttccccca aacggttgga ttggggtcgg
     8761 agatctgcac gcaaaggtgc tccaatttct tcccgcactg ggtcagcttt agcttgccgc
     8821 ccttctctgc caaggcttgg agagtggctt gccgctccgc tatcaattcc ccctgatacc
     8881 agaggatccc gctcgttgcg ctcaagaaca ggacgatgct gaccagcatc cagatccacg
     8941 ttttgagcaa cagccagccc attttctggc tgtgctcggc aatagcggcg ctcattttct
     9001 cctcgcttga tttcaacgcc ccggagatag ctttctcgtg cctcgtgaat tcttcggtca
     9061 gacgcttctc gatagcgttg gcctgctgct tcgagctctg ctcgaagtcc ttcgccatgt
     9121 ctaaaatctt gctcatacag cacccccttt aatcggatgt tccgcccccc gtccgggtcg
     9181 gcaatgctca ggctgttttt ggtttcgcgt accacgctga aacccgcacc ctgcaaggct
     9241 ttgcgcacat catcgcggct ctggatctcc cctgctccgg ccatgcgtaa aagcccgtct
     9301 gtaatggcct gagcggcctc cttgtgcccc ctcggcaaat cctttggcgt gataacggcc
     9361 tgacggttta acggatcgtc cgggtcgtgc aatttcagct ccccgtttac cacggttcgc
     9421 cacgcatcga ggcgcggatt gtcggccttg tggaagtagg gttgcagccg cttcccgctc
     9481 tgcaattcga tgttcgggac tacaaaattc agctccagcc gccccttgtc gcggtgctct
     9541 acccacaaaa cagcgtactg gttgccctcc agtccgggca ggagcgcctt ttcaaagtcg
     9601 ctcatcagct tgtccttggt cgccctgggt aaatcagcct cggcaaacga cagaacgcca
     9661 gaggtgtatt ttttggcgta gggactggaa tcaatcaggg cttcaatcac cgcagggttg
     9721 ccccggtcga gcgttgcgcc ttcccggttc ccgtcacggc ccaacaagta gccaacaggc
     9781 ccggagcccc ggcctacccc acggttatgg aatttgatga tcatggtgca gttcctccag
     9841 ctcgcggcct atagcggaca gctccgctat gatgcgcagc cgatccaacg gcccccactc
     9901 cccggaatta acgcgccggg cgatctggtt caggttgttg cccattccgg ccaactgccg
     9961 caagagcgcc ggatctacgg taggcgccgg cgttcggcgc tggggttgcg caacaccgag
    10021 acattgctcc cgcatccact ccgccagttg ggccttgggg cagagctccc gcaggcgctc
    10081 aagctccgca tctgagaccc ggatcttgat agagcggagg cgctttaacg gctggccagc
    10141 catgcagagg cctccgctaa ggcgtcctca tagaggctat cgagtagcgt ctggagcgcc
    10201 ttttcatccc gaaaatcgca gtgctccggc aaggggaaaa cacccccgcc gacctcttcg
    10261 ccgttctcca gcaaacgcag attccagccc ccgccaagac cgagagtgcg gggggtgatt
    10321 tcgtagtcgt attccgttgt tgtgggttcg tcacttacca tagctaacac ctcatcggtg
    10381 gaaatttttc cattgatata atcttcaagt agaattttga attcatcggt tatggcctcc
    10441 ccctctagtt ggaaactaga aataacgcca ttgactgcct tccttctcga atctttctct
    10501 atctgtgttg ttgacatagg gcctcgtcca ttctccagag ccaagaagcc accgcgcaga
    10561 ccgtcccgaa ggggcggatc gagcgggggc cacagggggt ttcggggggg cgtagcaccc
    10621 ctgaacaagg ccacagaggc ccgagaccgc gaagcaggta gaggggcctc tgtgtagcct
    10681 tgcttagtac attattcaac catgggcggg atactgcaag ccctagagca agatcaaggg
    10741 aaagagcggc tccgtggata actgctgccg cccacacccc ccggaaaaaa caggggggcg
    10801 atggacggac aggcgcagtt gtccacctcg ccaaagatcc gcgctaaaag gattttttaa
    10861 ggctaaaaga caagagcgca ggaacccccc taaagggggc tattatctga taaggcttgt
    10921 ttcgatcacg gacgatcacg ctttaggtaa acgctcaccg tactggggga gatttgaaga
    10981 tcgtccgcta tatcgcggtt ggtgtacccc tgagccttaa gccgcagaac ctccggcaat
    11041 agatcggact tgtccttacc gctattgctc ggccttcccc ctcccttgga taccttgccg
    11101 cccttcgcac ctctccgggc ctgctcctct ctgaaccctg cggggcttag gtgctggtgc
    11161 gtccatttgg caatggactt ggccgtgtgc cggacttcgt ttgcggggag cggctgctcg
    11221 aactgggcgt tatatcctga tgcccggtca acacaagcgg ccaaccattc ggggtaatcc
    11281 ggccagccct gccggatcgc tttataggcc cacaggcgcg ttttctcgaa cagggtacag
    11341 tttcgcccaa gcccgtactc aggaagcgat ttgcggccat tgtaggagct cagatcgagg
    11401 taatcggata gccaggctaa atcgtagagc cggggttccc atacctgcac cagccagtgc
    11461 tcatgcaggg ggttcttgca gatcagcccg gagtagccca tatccgcccc taatttctcc
    11521 cggagcgcgg cctcgatggc ggcggcatac cgcagagggg ctgcatttcc atccggggca
    11581 gtgcggatcg gcgtatccag tccgtagatc agatgcgcgt ggccgttggc cttgttcgtg
    11641 acggtaatgt taggggctgg agcgcctacg tcatcccaag ataacgtagc ggctgcatgg
    11701 tctacgtcaa aaacgatcca gtggcggtgc gttggcccgt tcggctggat gtaacgcgca
    11761 ccaagggcgc gggcaacatc cgcaatccgt aggcccgttg taagctcgtc ggaatagtag
    11821 ggtttccggg gcaggcgctc ccgaaacagc tcaagctgtg cgcactttct caagcaccac
    11881 cgcgcttatt ggcggcgtgg ggttgtttgg catgatccat gtgatagact tctcttgtga
    11941 ttcgtcacgg cccccgtttt tcgcagctcc gccaagatgc aagcgataac ggggggttgt
    12001 cttttctgga caatacgtta cagcaggcac atcctagaga gcaacgttag ttgcatgtaa
    12061 cctttcaccc tggaacatat ttccacctcc ttctgcttct gcgctgtttt gaattgcttg
    12121 atctacgcct ctaactaggt cgatccttgg gattgagtgg caccattgtc agtgcaagaa
    12181 tactgatcca tataagtctc tctagtcgga caacaccaag tgttgaagag atcaaaaatc
    12241 aacctatatc ggaggattca tgtctgacat tgacagatgc ccc
//
