LOCUS       NZ_CP141572            19463 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP35_L8_42.22_ST2096_OXA232 plasmid
            pK35_unnamed1, complete sequence.
ACCESSION   NZ_CP141572
VERSION     NZ_CP141572.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38771710
            Assembly: GCF_035598475.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 19463)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19463)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Assembly Name                     :: rrp8_bc10
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 11:39:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,344
            CDSs (total)                      :: 5,222
            Genes (coding)                    :: 5,024
            CDSs (with protein)               :: 5,024
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 118 of 198
            Pseudo Genes (incomplete)         :: 76 of 198
            Pseudo Genes (internal stop)      :: 26 of 198
            Pseudo Genes (multiple problems)  :: 20 of 198
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141572.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..19463
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP35_L8_42.22_ST2096_OXA232"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pK35_unnamed1"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-09-03"
                     /genotype="ST2096"
     gene            complement(join(19321..19463,1..123))
                     /locus_tag="VI604_RS00510"
                     /old_locus_tag="VI604_00510"
                     /pseudo=""
     CDS             complement(join(19321..19463,1..123))
                     /locus_tag="VI604_RS00510"
                     /old_locus_tag="VI604_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /name="plasmid stabilization protein"
     gene            500..1585
                     /locus_tag="VI604_RS00515"
                     /old_locus_tag="VI604_00515"
     CDS             500..1585
                     /locus_tag="VI604_RS00515"
                     /old_locus_tag="VI604_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            1582..3771
                     /locus_tag="VI604_RS00520"
                     /old_locus_tag="VI604_00520"
     CDS             1582..3771
                     /locus_tag="VI604_RS00520"
                     /old_locus_tag="VI604_00520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            5357..7318
                     /locus_tag="VI604_RS00525"
                     /old_locus_tag="VI604_00525"
     CDS             5357..7318
                     /locus_tag="VI604_RS00525"
                     /old_locus_tag="VI604_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            7318..8049
                     /gene="mobC"
                     /locus_tag="VI604_RS00530"
                     /old_locus_tag="VI604_00530"
     CDS             7318..8049
                     /gene="mobC"
                     /locus_tag="VI604_RS00530"
                     /old_locus_tag="VI604_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            8056..8586
                     /locus_tag="VI604_RS00535"
                     /old_locus_tag="VI604_00535"
     CDS             8056..8586
                     /locus_tag="VI604_RS00535"
                     /old_locus_tag="VI604_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8614..8793)
                     /locus_tag="VI604_RS00540"
                     /old_locus_tag="VI604_00540"
     CDS             complement(8614..8793)
                     /locus_tag="VI604_RS00540"
                     /old_locus_tag="VI604_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8862..9239
                     /locus_tag="VI604_RS00545"
                     /old_locus_tag="VI604_00545"
     CDS             8862..9239
                     /locus_tag="VI604_RS00545"
                     /old_locus_tag="VI604_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(9301..9600)
                     /locus_tag="VI604_RS00550"
                     /old_locus_tag="VI604_00550"
     CDS             complement(9301..9600)
                     /locus_tag="VI604_RS00550"
                     /old_locus_tag="VI604_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(9590..9853)
                     /locus_tag="VI604_RS00555"
                     /old_locus_tag="VI604_00555"
     CDS             complement(9590..9853)
                     /locus_tag="VI604_RS00555"
                     /old_locus_tag="VI604_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            10230..11315
                     /locus_tag="VI604_RS00560"
                     /old_locus_tag="VI604_00560"
     CDS             10230..11315
                     /locus_tag="VI604_RS00560"
                     /old_locus_tag="VI604_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            11312..13501
                     /locus_tag="VI604_RS00565"
                     /old_locus_tag="VI604_00565"
     CDS             11312..13501
                     /locus_tag="VI604_RS00565"
                     /old_locus_tag="VI604_00565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            15087..17048
                     /locus_tag="VI604_RS00570"
                     /old_locus_tag="VI604_00570"
     CDS             15087..17048
                     /locus_tag="VI604_RS00570"
                     /old_locus_tag="VI604_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            17048..17779
                     /gene="mobC"
                     /locus_tag="VI604_RS00575"
                     /old_locus_tag="VI604_00575"
     CDS             17048..17779
                     /gene="mobC"
                     /locus_tag="VI604_RS00575"
                     /old_locus_tag="VI604_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            17786..18316
                     /locus_tag="VI604_RS00580"
                     /old_locus_tag="VI604_00580"
     CDS             17786..18316
                     /locus_tag="VI604_RS00580"
                     /old_locus_tag="VI604_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(18344..18523)
                     /locus_tag="VI604_RS00585"
                     /old_locus_tag="VI604_00585"
     CDS             complement(18344..18523)
                     /locus_tag="VI604_RS00585"
                     /old_locus_tag="VI604_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            18592..18969
                     /locus_tag="VI604_RS00590"
                     /old_locus_tag="VI604_00590"
     CDS             18592..18969
                     /locus_tag="VI604_RS00590"
                     /old_locus_tag="VI604_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_324649010.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRRIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(19031..19331)
                     /locus_tag="VI604_RS00595"
                     /old_locus_tag="VI604_00595"
                     /pseudo=""
     CDS             complement(19031..19331)
                     /locus_tag="VI604_RS00595"
                     /old_locus_tag="VI604_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     Typing          complement(3977..4557)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(13707..14287)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7319..8046
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.93e-144"
     Typing          17049..17776
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.93e-144"
ORIGIN
        1 cttcgttagc caggcgctgc aacagtgcgg cttttccggg ccaccttcag gcagtatgcg
       61 gggcagtctc tcggccagtt cccgcaggaa attggcgttt tcatagtgtg tactgttgct
      121 cattgtggag cctcccgggc tgatacgtcg atatgaagga gtttagcagg gggcggtctg
      181 aaagaatatg ctcgtgtcag tcaggctgat gaagacgaaa gctggtaaaa aagggcttac
      241 gacacccatt ttacgtcaaa ctgcacgaca tgcagttgcg tgcatagcca tgcagcgggt
      301 taaaatttat caggcgcgtt tccggcggtt ttccgggtgg tttgttgccg gttttatccc
      361 gtagccgccg gaaatgccct cagaccgtct gagcggtgcg cgtaatgcga cgttatggta
      421 aatcatgtga ttaatggtag tgataatcat tatcactttt gctatattct tgtttctttt
      481 attgtaagga aatgtaagta tggcaatcac gattaaatcc gccgttaatg tccgtagcag
      541 agtattaata tactctttac tactgtttac tggttcagcg tatgcggctg atatagctga
      601 atcaacattc ctgactcgtg atatcggcaa tggtctgtat gaaatgtctt ttgatggcaa
      661 aaataatagt ctctgggcag catcatcgtc ttcattcgac agagataagg gccacggaat
      721 tgtttatcag ttagaaccat ccactctgaa aacaaaatct gccgttaaaa tcgcacgtct
      781 tgggtttgcc acagcactgg atgaggaaca tgagcgactt tatatcggca atactctgga
      841 cggctcagtg acggtcattg atactctaac cggaaaagaa ctttcagtaa ttccgttaac
      901 tgagggacag gatgaggctc atttcagacc gaccagagaa atggtcattg acaaaaaaaa
      961 tcagcgtctt tatgtctcca gcgccgcaga taaaggggct ctgtgggtta ttgacatggc
     1021 tgtcgggaag aaaattaaaa ccattgatgg actggggcac gctgttactg gaattgcgtt
     1081 aaataaaaat ggggataata tttatctggt taacggggat ggtgagatta ttaacctgga
     1141 tgccagtagc tatgaaataa aaaaacggtt tgttgtagag cctgagaaaa aacacttttt
     1201 ccttaatgta agtctggatg aaaaaaacgg acgtgcattt attaccgacc cggatattcc
     1261 cggggtgctg gttgttgatg taaactcggg aaaaatacta cacagaattg atgttataaa
     1321 ttcactggcg attttattta atcctttacg gaacgaaatc tatatcacgc accgtaatgc
     1381 aaaacaggtc agcattgtgg acagtaattc ttattacgta aaaacaacga taaaaacccg
     1441 cctgatgcca aacagtctgt ctttatcaca tgacggtgaa aatctgtacg ttagtgtaaa
     1501 acaagaaaaa aaagagatga gtactatgaa ggattacata ctcaaaatcg aattatctaa
     1561 aattgctatg gagccagaag aatgaataaa tatttagggc tgagcatagt atatatgatg
     1621 tatacatcat ctgttatggc tgatgattct ggcgcagtta gtaaagataa aaatatttca
     1681 ccagaagaaa cattaattgt tcatgggacc gctgtttctg aaagcacaac agaaggaaca
     1741 gattcataca caacccagtc agccaataca gcatcaaaga tgaatctgtc attccgggat
     1801 acacctcagt caactacagt cattacgcga cgaagaatgg atgatgtagc agcaaaaaat
     1861 attattgatg tggttaaaac cgctcctgga ttatatgtca gtacagcaga aggttccgga
     1921 agaccgaatt ttatgtccag aggatttaat gctaatgtca tgtatgaagg atttagcctg
     1981 ccgtggaatt catacattcc aagttcacaa actagttcag ctatttttga ccgggtggag
     2041 atagtccgcg gggcaacagg tcttgttcag ggtgccggag acccttcagc cgcgataaat
     2101 atgatacaca agcgtccgac ccggcaatat caggccagca ctaacatcgg tgccggacgg
     2161 tggaatgact accggggtgg ggttgatgta tcaggaccac tgaatgacag cgcgacctta
     2221 cgcggaaggg cagttgtgga tggcgaaaac agtgagacct tcagggatag tgagaaacgc
     2281 aggaataacg tcatttacgg aattatggag gcagatctga ctgataatac cctacttacg
     2341 attgggggat atcagcagta tgaatataac aatcattggt ggtatgactt acctatttct
     2401 ggtaccggac accaccttaa tcttcctcgc tcgacgttta caggcaatga ctgggagttt
     2461 gaaagaagta aaagtaaaac tgcatttgca actcttgagc agaagttcag taatagctgg
     2521 aagctgaaat tttcaacgct gcaaaactgg agaaatgctg acctgttagg gacggcaaca
     2581 taccggcata aagatacccg acaaagtatg tatcagcaag tatggggatc actgagggaa
     2641 tataattcca aaagctatga cctgtcgctt aatggggact atgctttatt tggtcgtgtt
     2701 catcaattcg tggctggaac aactcagcaa aaatacaccg ataaagatac aggttataca
     2761 tattccccaa tatatattga gaatgttaat atatttgatc atggttcaca ttcttcgaaa
     2821 aaacccatga agatgtcagg tgatgcatcc tacgacgtca ctgaacaaaa aagcgcctac
     2881 gcagcaactc attttctact tagtgataac tggaaaatat tgctgggtgg ccgtcttgac
     2941 tggtatgaat atacaagtaa aggtacatcc attaatacag gttataaagt taccagaaat
     3001 atcaccaaat atgcaggttc aacatacaat cttgacgatc atcataccat ttatgggagc
     3061 tataccgata tattcaatcc acagtcaagc accgacctca atggtaaagt gatcaagcct
     3121 attgttggta agaattatga gacaggtatt aagggtgagt attttaacgg cttgcttaat
     3181 gcctctctgg cggtatttca ggtagaccag gaaaatcgag ccaggctgct tcccgatcag
     3241 acagggtgtg ttactaatgg caatcctcaa tgctatgagg ctagcggtaa agtacgcaca
     3301 aaagggatag atttcgaact tcagggcatg gttacgccac tctgggaaat tggggtggga
     3361 tatacatatg cagatacgcg atatataaaa gatggagatc gtagtaatga aggcaagcga
     3421 ttcaatactg ccgtgtcgcc acaaaattta tttaagattt cgagcatgta cacctttgac
     3481 ggaaaattaa caaactggcg agcaggagta aacacgtact ggcaaagtaa aatatatgct
     3541 aacataacag acacaaggga tggcaatccg gttattaata aacaaaaggc ttattcaaca
     3601 actgacatta ttattggcta tactccaatg ccacaactgg atttacagct caatataact
     3661 aatgtctttg ataaggttta ttatacatcc ataggtgaat cgattggata tggcacggaa
     3721 acatatggag aaccaagaaa tgttaaaatt agtgcaacct atagattcta accatgcgga
     3781 ttgggtcttc tcctcggatt tgcccctata aatccgtctt ttgtggttcc ggcgtacaac
     3841 cccctgtttg ccacttcagt tgatggttaa tcaaatcatg tagaggatct tcttgagatc
     3901 ctccatggtt cgtcgtaatc ttttgccctg taaacgaaaa aaccacctgg ggaggtggtt
     3961 tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct ttcgcagagc
     4021 acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt caagagcaac
     4081 cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct gctgccagtg
     4141 gcgtttttgc gtgtctttcc gggttggact caagtgaaca gttaccggat aaggcgcagc
     4201 agtcgggctg aacggggagt tcttgcttac agcccagctt ggagcgaacg acctacaccg
     4261 agccgagata ccagtgtgtg agctatgaga aagcgccaca cttcccgtaa gggagaaagg
     4321 cggaacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag ggagcgaccc
     4381 gccggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt cagattcatg
     4441 gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt gaattctggc
     4501 aggagggcga agcctatgga aaaacgccac cggcgcggcc ctgccgtttt gcctcacatg
     4561 ttagtcccct gcttatccac ggaatctgtg ggtaactttg cgagagttag cagcgcccgc
     4621 cgcagtctca cggccggagc gtagcgactg agtgagggag gaggcgcaat atcccccctc
     4681 ccgcagacgg ccaccggacg cgcggatgtg acatattgca ccgtgttacc gcctttccct
     4741 tccctgccga taaatcattc agataggtga agtctgtcgg ttttctgctg aatattttac
     4801 gttaacctat ctctgttgct tttttctcct tgcggagaaa tgcaggtcat ccccgctggg
     4861 gcgtggctga cttcaggagg aaatcccctg cttacgctgg ctggacgcca ccgacgcacg
     4921 gtctttcccc ctctttctgg ctcagctcgg ctggacgccg gccacgccag aaattcaccc
     4981 ggcccccgtc aggctcacgc ctgaccaccc acaaaaaccc acggtgctga acgcacctga
     5041 cgaagcagca aagctgctcc tgattttgcg ggtggtcagc cgctcgctat ggcgaactct
     5101 gacgggggcc ggaacaccgg aaaagaccgc actggggtgc cgccttttcc tctcacacgc
     5161 tggggcgtgc tcgttgttcc tccggggctg cgccttttcc gcttggggcg gaaagaggct
     5221 ggcctccgtg gtgaaagctg aatgcaatca ccacggacgt gaccgccgcc gccatgcctc
     5281 ggggcatgtc cgcggcttgt gcagcagggg ctgcacacca cgccggtaaa cccgtcgtct
     5341 gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc tggcagtggc atatcccgga
     5401 gctggcccgg cagatttacc cgtgggtcag ctggctgtac agggagtatt acggtctggc
     5461 cggggctttc ctggttatcg gcctgttttt cggtctggct gaactctggg ccggacggat
     5521 gcgttttgtg gggctgagtc ataaggagcg acgggagtat gccgatcgct ataagcgcga
     5581 taataaccgg actgcgccat tttcggtgct ggcttcgctg gattcgcccg ggcggatggt
     5641 cagggcacgg cggcatgtct ggaactacgc gtactggctg accggcgctg ccggtctggc
     5701 ccttctgggg gggctggttg tcgccgtgtt tatcgagagc tggggagaaa ccgtcctgcg
     5761 tccggacctc tacgcgaacg cagattatta cctgctgcag ccggctaacc tgttcgggat
     5821 tgtctggtgt gccggagggc ttgtcgccgg catccttctg gcgcggctgc tggcatttct
     5881 gctgcttgac ggtcattttg cggccgctga cgaggcggtg aatgcgaaac tgaaccagga
     5941 gagccggcgc agcagccagc ggaccggaga aatgaccgac gtccgccacc tgcatttcgg
     6001 ggagccggta ccggttaacg cgctggccga tttttccaca gagcaggccc ggaaacagca
     6061 ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg ctggtcccgc gtgatacctg
     6121 gcgcaagacc aatattcaga tcctcggcct gcccgggagc ggcaagagcg tgatgggcac
     6181 caacgcgctc attcgctgcg tacgcgattt cggggacgcg gtggtgtact tcgaccccaa
     6241 aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc ccggacttca ccctgctgga
     6301 tctgcggccg gggaaaccgg cgcagctgaa tctgtttcgc gatctcgacc agtacgccct
     6361 gaagaatctg ctggttgccg ggtttaacct gagtgagacc ggcgacgtgg cggaccacta
     6421 ccgcatcagc gagcagaaag cggcgaagct gattgcggag cagttcccgc agggggcaaa
     6481 cattcagcag gtgctggcgg cggcatacgc gctgccggag gccctgaaaa aggacgtgaa
     6541 ggggctgatc accaagctgg agaacgtggc cgacctgagc gtcctgcaga cggacagcgg
     6601 catcgacgtg gcggggataa tcaacggcgg cggctgcctg tacgtcatcg gctcgatgga
     6661 cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc cgctgcgcgc aaatcatcat
     6721 tgcgcgggat gagttccgga ggtggccgca cgcgagcatc atgctcgatg agattaaata
     6781 cctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg cgcagtcggg actgcaacct
     6841 gctgctcgcg caccagtcgc tgggcgactt cgggcagtgc ggccaggatc tgcccgctga
     6901 gtttgtgaaa accacggtgc tggataacac gccgatccgc tggttctacc gggcggccag
     6961 ccaggagtcc gcgcagtggg cggcgggcca gaccggggaa atccgggtgg acgtggagcg
     7021 ccgccgggcc agccgggagg cggggaacgt ggagcatatc agcggggaca gcttcattca
     7081 gaaggaggcg cggccgctgt ttgacgtgaa caccctgcag cacctgcctg acgggtttgc
     7141 ggtgatgacc gggctgggcg tggcccggat ggcgttcagc agcccgctgc gggttgaacg
     7201 ccgggaaatc ccgctgcagc gcttcccggt gctggcgaaa acggacccgc tggcggagta
     7261 ccagccggag gccgggcggc agcggccggg tgatgatgat tttgcggggc tgtactgatg
     7321 gcactggagc gatacaacgt cagccacgcg aagcggcagg cgcggaacgc ggagaaaacc
     7381 cggctgaccc tgcgctggct gcgggaagag ctgtgttcca cggcggagct ggttgcgcga
     7441 cggctgggga ttgcggcggt gcagccggtt taccgcttcc tggacagcct ggtggcgaaa
     7501 gggctgctgg tccgggcgaa atacccggtt gacggccgtc aggtcagcgt gtggggactg
     7561 acgccgcacg gggtggcgtt ctcgttcgac gaggacgaac cgctgacgga cgttattccg
     7621 tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc ggctggccgt gcagtccctg
     7681 cgtctcgcca tggaggcccg gggggcctcc ggctggcggt acctgcaccg tatggccctg
     7741 aaggggatga aggtgccgga cgcgctggcg gagctggacg gcagaacggt ggcgtttgag
     7801 gtggagcgca ccgtgaagag ccgcaggcgc tatcaggagg tggtgtccgg gtatctgttc
     7861 aatcgcaaag cgaacggcat cgatgaaatc tggtacatct gcccggaccg ggcgacgcag
     7921 gtcagggtgc agcgggcgat cctgtcggtg gatgaaatcg tgaatccaca gaccggcgag
     7981 gcccgcaaaa cggcggagct ggaccgggag cggctgtttg cgtgctttaa atttatgaca
     8041 acggagtgag ggattatggc gctgtacatg gtatttattg aagacgcgga ggaccgggac
     8101 ggcgagcgct gcatcatgcc gaccagcgag catcttgaag atatggtggt gctcgcctgg
     8161 cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc agcatctggc gatgatgaaa
     8221 tccatcggga tgtatgacga gcggctcaac agcgacgaaa aagccacgta ctttttcacc
     8281 ggtaaaaatc cgcaatggga acgggacaaa cccgcaggaa tgaagctgtg tgattacctc
     8341 tatcgcaagg tggagcggtt cgccatcgag aacggtgtgc aggtggttga tgccgggaag
     8401 tgctcaccgg agcagattag cgcgatgatt gaggtggtca gcgagcggca cggggaaagc
     8461 gcggtgaacg agaagctgaa agcggagctg atgcgggaat ataacagccg ctattttaac
     8521 gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc gttcggcaaa
     8581 aaataaacgg cagcaccggc catgccggtg ctgttattcc ttcccgagct tatcccgcat
     8641 cacctgccag agtgtttccg catcctcgtg cagccgctcg cacatgtcgg ccggggtatc
     8701 catatccagc tcgttcagtt tctcgagtaa cagcagcgac tggtccttaa taaatttagc
     8761 catattgagg gcagtttttt cctgcttagt catgttttct gtccttccgg gtgtggtgtg
     8821 cttcctgtcg ggtatactgt ctcaaaaccg gaggtgagac aatggcgtgg gtgacggtga
     8881 gacaggcgac ggaactgacg gggaaagccc ggagttccct gtatagggat atggcgaaag
     8941 gccgcgtatc ataccgtact gaagctgatg gtggtcgggt ggtggatacc agcgaactta
     9001 tccgggtgta cggtgaatta agacagattg agactcacga gcgggacggg ttgagactgt
     9061 cggatgagac tgaaaaaatg gcctccgacg cgttgatcgc ggagataaaa gcactcaggg
     9121 aggaggtggc cggactgcgg caggaaatgc aggccatgag actgctggaa cacaaaccag
     9181 agacagcaga gacaccggcc ggaacatcgc gccgatggtg gcagtgggga aaacgctgac
     9241 gcgctgtgct tgtccaaccg gcgacggcca cgcaatgaat tgcgccccca tcaccggtta
     9301 tcagcgaacg gtatggggat tctggcgctg gccaaccacg gccagaaccc ggactttcct
     9361 ggtctggtca tcaacatcat agaggacatg ctgtcccatc agactgatcc ggcgaacgtc
     9421 ctcgccatag cgtgagtcca cttcatatag gcgcgggaag tcggccagtt tgtccgctgc
     9481 agcgagaact cgatcggtaa cgttctgcgc atcccacagg gatgaatttt ccgcaatgta
     9541 gtcaaacaat gctgtcacat cctcgatggc tccctgcttc cattccacct catacggcat
     9601 cgcgtagttc ctgatagcgg ccatgcagtc tctggcggag ttgttccgtg gtcatgcccg
     9661 ggcgcgtgtc agcgcgggcg gcggtcactt tagcgcgaac ctgatcttca tattctgcct
     9721 gggccagttc ttcgttagcc aggcgctgca acagtgcagc tttgtccggg ccaccttcag
     9781 gcagtatgcg gggcagtctc tcggccagtt cccgcaggaa attggcgttt tcatagtgtg
     9841 tactgttgct cattgtggag cctcccgggc tgatacgtcg atatgaagga gtttagcagg
     9901 gggcggtctg aaagaatatg ctcgtgtcag tcaggctgat gaagacgaaa gctggtaaaa
     9961 aagggcttac gacacccatt ttacgtcaaa ctgcacgaca tgcagttgcg tgcatagcca
    10021 tgcagcgggt taaaatttat caggcgcgtt tccggcggtt ttccgggtgg tttgttgccg
    10081 gttttatccc gtagccgccg gaaatgccct cagaccgtct gagcggtgcg cgtaatgcga
    10141 cgttatggta aatcatgtga ttaatggtag tgataatcat tatcactttt gctatattct
    10201 tgtttctttt attgtaagga aatgtaagta tggcaatcac gattaaatcc gccgttaatg
    10261 tccgtagcag agtattaata tactctttac tactgtttac tggttcagcg tatgcggctg
    10321 atatagctga atcaacattc ctgactcgtg atatcggcaa tggtctgtat gaaatgtctt
    10381 ttgatggcaa aaataatagt ctctgggcag catcatcgtc ttcattcgac agagataagg
    10441 gccacggaat tgtttatcag ttagaaccat ccactctgaa aacaaaatct gccgttaaaa
    10501 tcgcacgtct tgggtttgcc acagcactgg atgaggaaca tgagcgactt tatatcggca
    10561 atactctgga cggctcagtg acggtcattg atactctaac cggaaaagaa ctttcagtaa
    10621 ttccgttaac tgagggacag gatgaggctc atttcagacc gaccagagaa atggtcattg
    10681 acaaaaaaaa tcagcgtctt tatgtctcca gcgccgcaga taaaggggct ctgtgggtta
    10741 ttgacatggc tgtcgggaag aaaattaaaa ccattgatgg actggggcac gctgttactg
    10801 gaattgcgtt aaataaaaat ggggataata tttatctggt taacggggat ggtgagatta
    10861 ttaacctgga tgccagtagc tatgaaataa aaaaacggtt tgttgtagag cctgagaaaa
    10921 aacacttttt ccttaatgta agtctggatg aaaaaaacgg acgtgcattt attaccgacc
    10981 cggatattcc cggggtgctg gttgttgatg taaactcggg aaaaatacta cacagaattg
    11041 atgttataaa ttcactggcg attttattta atcctttacg gaacgaaatc tatatcacgc
    11101 accgtaatgc aaaacaggtc agcattgtgg acagtaattc ttattacgta aaaacaacga
    11161 taaaaacccg cctgatgcca aacagtctgt ctttatcaca tgacggtgaa aatctgtacg
    11221 ttagtgtaaa acaagaaaaa aaagagatga gtactatgaa ggattacata ctcaaaatcg
    11281 aattatctaa aattgctatg gagccagaag aatgaataaa tatttagggc tgagcatagt
    11341 atatatgatg tatacatcat ctgttatggc tgatgattct ggcgcagtta gtaaagataa
    11401 aaatatttca ccagaagaaa cattaattgt tcatgggacc gctgtttctg aaagcacaac
    11461 agaaggaaca gattcataca caacccagtc agccaataca gcatcaaaga tgaatctgtc
    11521 attccgggat acacctcagt caactacagt cattacgcga cgaagaatgg atgatgtagc
    11581 agcaaaaaat attattgatg tggttaaaac cgctcctgga ttatatgtca gtacagcaga
    11641 aggttccgga agaccgaatt ttatgtccag aggatttaat gctaatgtca tgtatgaagg
    11701 atttagcctg ccgtggaatt catacattcc aagttcacaa actagttcag ctatttttga
    11761 ccgggtggag atagtccgcg gggcaacagg tcttgttcag ggtgccggag acccttcagc
    11821 cgcgataaat atgatacaca agcgtccgac ccggcaatat caggccagca ctaacatcgg
    11881 tgccggacgg tggaatgact accggggtgg ggttgatgta tcaggaccac tgaatgacag
    11941 cgcgacctta cgcggaaggg cagttgtgga tggcgaaaac agtgagacct tcagggatag
    12001 tgagaaacgc aggaataacg tcatttacgg aattatggag gcagatctga ctgataatac
    12061 cctacttacg attgggggat atcagcagta tgaatataac aatcattggt ggtatgactt
    12121 acctatttct ggtaccggac accaccttaa tcttcctcgc tcgacgttta caggcaatga
    12181 ctgggagttt gaaagaagta aaagtaaaac tgcatttgca actcttgagc agaagttcag
    12241 taatagctgg aagctgaaat tttcaacgct gcaaaactgg agaaatgctg acctgttagg
    12301 gacggcaaca taccggcata aagatacccg acaaagtatg tatcagcaag tatggggatc
    12361 actgagggaa tataattcca aaagctatga cctgtcgctt aatggggact atgctttatt
    12421 tggtcgtgtt catcaattcg tggctggaac aactcagcaa aaatacaccg ataaagatac
    12481 aggttataca tattccccaa tatatattga gaatgttaat atatttgatc atggttcaca
    12541 ttcttcgaaa aaacccatga agatgtcagg tgatgcatcc tacgacgtca ctgaacaaaa
    12601 aagcgcctac gcagcaactc attttctact tagtgataac tggaaaatat tgctgggtgg
    12661 ccgtcttgac tggtatgaat atacaagtaa aggtacatcc attaatacag gttataaagt
    12721 taccagaaat atcaccaaat atgcaggttc aacatacaat cttgacgatc atcataccat
    12781 ttatgggagc tataccgata tattcaatcc acagtcaagc accgacctca atggtaaagt
    12841 gatcaagcct attgttggta agaattatga gacaggtatt aagggtgagt attttaacgg
    12901 cttgcttaat gcctctctgg cggtatttca ggtagaccag gaaaatcgag ccaggctgct
    12961 tcccgatcag acagggtgtg ttactaatgg caatcctcaa tgctatgagg ctagcggtaa
    13021 agtacgcaca aaagggatag atttcgaact tcagggcatg gttacgccac tctgggaaat
    13081 tggggtggga tatacatatg cagatacgcg atatataaaa gatggagatc gtagtaatga
    13141 aggcaagcga ttcaatactg ccgtgtcgcc acaaaattta tttaagattt cgagcatgta
    13201 cacctttgac ggaaaattaa caaactggcg agcaggagta aacacgtact ggcaaagtaa
    13261 aatatatgct aacataacag acacaaggga tggcaatccg gttattaata aacaaaaggc
    13321 ttattcaaca actgacatta ttattggcta tactccaatg ccacaactgg atttacagct
    13381 caatataact aatgtctttg ataaggttta ttatacatcc ataggtgaat cgattggata
    13441 tggcacggaa acatatggag aaccaagaaa tgttaaaatt agtgcaacct atagattcta
    13501 accatgcgga ttgggtcttc tcctcggatt tgcccctata aatccgtctt ttgtggttcc
    13561 ggcgtacaac cccctgtttg ccacttcagt tgatggttaa tcaaatcatg tagaggatct
    13621 tcttgagatc ctccatggtt cgtcgtaatc ttttgccctg taaacgaaaa aaccacctgg
    13681 ggaggtggtt tgatcgaagg ttaagtcagt tggggaactg cttaaccgtg gtaactggct
    13741 ttcgcagagc acagcaacca aatctgtcct tccagtgtag ccggactttg gcgcacactt
    13801 caagagcaac cgcgtgttta gctaaacaaa tcctctgcga actcccagtt accaatggct
    13861 gctgccagtg gcgtttttgc gtgtctttcc gggttggact caagtgaaca gttaccggat
    13921 aaggcgcagc agtcgggctg aacggggagt tcttgcttac agcccagctt ggagcgaacg
    13981 acctacaccg agccgagata ccagtgtgtg agctatgaga aagcgccaca cttcccgtaa
    14041 gggagaaagg cggaacaggt atccggtaaa cggcagggtc ggaacaggag agcgcaagag
    14101 ggagcgaccc gccggaaacg gtggggatct ttaagtcctg tcgggtttcg cccgtactgt
    14161 cagattcatg gttgagcctc acggctccca cagatgcacc gaaaaagcgt ctgtttatgt
    14221 gaattctggc aggagggcga agcctatgga aaaacgccac cggcgcggcc ctgccgtttt
    14281 gcctcacatg ttagtcccct gcttatccac ggaatctgtg ggtaactttg cgagagttag
    14341 cagcgcccgc cgcagtctca cggccggagc gtagcgactg agtgagggag gaggcgcaat
    14401 atcccccctc ccgcagacgg ccaccggacg cgcggatgtg acatattgca ccgtgttacc
    14461 gcctttccct tccctgccga taaatcattc agataggtga agtctgtcgg ttttctgctg
    14521 aatattttac gttaacctat ctctgttgct tttttctcct tgcggagaaa tgcaggtcat
    14581 ccccgctggg gcgtggctga cttcaggagg aaatcccctg cttacgctgg ctggacgcca
    14641 ccgacgcacg gtctttcccc ctctttctgg ctcagctcgg ctggacgccg gccacgccag
    14701 aaattcaccc ggcccccgtc aggctcacgc ctgaccaccc acaaaaaccc acggtgctga
    14761 acgcacctga cgaagcagca aagctgctcc tgattttgcg ggtggtcagc cgctcgctat
    14821 ggcgaactct gacgggggcc ggaacaccgg aaaagaccgc actggggtgc cgccttttcc
    14881 tctcacacgc tggggcgtgc tcgttgttcc tccggggctg cgccttttcc gcttggggcg
    14941 gaaagaggct ggcctccgtg gtgaaagctg aatgcaatca ccacggacgt gaccgccgcc
    15001 gccatgcctc ggggcatgtc cgcggcttgt gcagcagggg ctgcacacca cgccggtaaa
    15061 cccgtcgtct gaaggagaga gattgcatgt ttaatacgga ttcgcttgcc tggcagtggc
    15121 atatcccgga gctggcccgg cagatttacc cgtgggtcag ctggctgtac agggagtatt
    15181 acggtctggc cggggctttc ctggttatcg gcctgttttt cggtctggct gaactctggg
    15241 ccggacggat gcgttttgtg gggctgagtc ataaggagcg acgggagtat gccgatcgct
    15301 ataagcgcga taataaccgg actgcgccat tttcggtgct ggcttcgctg gattcgcccg
    15361 ggcggatggt cagggcacgg cggcatgtct ggaactacgc gtactggctg accggcgctg
    15421 ccggtctggc ccttctgggg gggctggttg tcgccgtgtt tatcgagagc tggggagaaa
    15481 ccgtcctgcg tccggacctc tacgcgaacg cagattatta cctgctgcag ccggctaacc
    15541 tgttcgggat tgtctggtgt gccggagggc ttgtcgccgg catccttctg gcgcggctgc
    15601 tggcatttct gctgcttgac ggtcattttg cggccgctga cgaggcggtg aatgcgaaac
    15661 tgaaccagga gagccggcgc agcagccagc ggaccggaga aatgaccgac gtccgccacc
    15721 tgcatttcgg ggagccggta ccggttaacg cgctggccga tttttccaca gagcaggccc
    15781 ggaaacagca ggcggtgttt ctgggtaagg atgagcaggg ccagccggtg ctggtcccgc
    15841 gtgatacctg gcgcaagacc aatattcaga tcctcggcct gcccgggagc ggcaagagcg
    15901 tgatgggcac caacgcgctc attcgctgcg tacgcgattt cggggacgcg gtggtgtact
    15961 tcgaccccaa aggggacgcg tgggcgccgc acgtcttccg ggcgcactgc ccggacttca
    16021 ccctgctgga tctgcggccg gggaaaccgg cgcagctgaa tctgtttcgc gatctcgacc
    16081 agtacgccct gaagaatctg ctggttgccg ggtttaacct gagtgagacc ggcgacgtgg
    16141 cggaccacta ccgcatcagc gagcagaaag cggcgaagct gattgcggag cagttcccgc
    16201 agggggcaaa cattcagcag gtgctggcgg cggcatacgc gctgccggag gccctgaaaa
    16261 aggacgtgaa ggggctgatc accaagctgg agaacgtggc cgacctgagc gtcctgcaga
    16321 cggacagcgg catcgacgtg gcggggataa tcaacggcgg cggctgcctg tacgtcatcg
    16381 gctcgatgga cgatgaggcg gtgatccggg tgcagaagat gctgtttgcc cgctgcgcgc
    16441 aaatcatcat tgcgcgggat gagttccgga ggtggccgca cgcgagcatc atgctcgatg
    16501 agattaaata cctgctgtcg aagtacgtgc tgaacgcgct ggggacgctg cgcagtcggg
    16561 actgcaacct gctgctcgcg caccagtcgc tgggcgactt cgggcagtgc ggccaggatc
    16621 tgcccgctga gtttgtgaaa accacggtgc tggataacac gccgatccgc tggttctacc
    16681 gggcggccag ccaggagtcc gcgcagtggg cggcgggcca gaccggggaa atccgggtgg
    16741 acgtggagcg ccgccgggcc agccgggagg cggggaacgt ggagcatatc agcggggaca
    16801 gcttcattca gaaggaggcg cggccgctgt ttgacgtgaa caccctgcag cacctgcctg
    16861 acgggtttgc ggtgatgacc gggctgggcg tggcccggat ggcgttcagc agcccgctgc
    16921 gggttgaacg ccgggaaatc ccgctgcagc gcttcccggt gctggcgaaa acggacccgc
    16981 tggcggagta ccagccggag gccgggcggc agcggccggg tgatgatgat tttgcggggc
    17041 tgtactgatg gcactggagc gatacaacgt cagccacgcg aagcggcagg cgcggaacgc
    17101 ggagaaaacc cggctgaccc tgcgctggct gcgggaagag ctgtgttcca cggcggagct
    17161 ggttgcgcga cggctgggga ttgcggcggt gcagccggtt taccgcttcc tggacagcct
    17221 ggtggcgaaa gggctgctgg tccgggcgaa atacccggtt gacggccgtc aggtcagcgt
    17281 gtggggactg acgccgcacg gggtggcgtt ctcgttcgac gaggacgaac cgctgacgga
    17341 cgttattccg tttcagccgt caagggtgtc ggcggcgcag ctgccgcacc ggctggccgt
    17401 gcagtccctg cgtctcgcca tggaggcccg gggggcctcc ggctggcggt acctgcaccg
    17461 tatggccctg aaggggatga aggtgccgga cgcgctggcg gagctggacg gcagaacggt
    17521 ggcgtttgag gtggagcgca ccgtgaagag ccgcaggcgc tatcaggagg tggtgtccgg
    17581 gtatctgttc aatcgcaaag cgaacggcat cgatgaaatc tggtacatct gcccggaccg
    17641 ggcgacgcag gtcagggtgc agcgggcgat cctgtcggtg gatgaaatcg tgaatccaca
    17701 gaccggcgag gcccgcaaaa cggcggagct ggaccgggag cggctgtttg cgtgctttaa
    17761 atttatgaca acggagtgag ggattatggc gctgtacatg gtatttattg aagacgcgga
    17821 ggaccgggac ggcgagcgct gcatcatgcc gaccagcgag catcttgaag atatggtggt
    17881 gctcgcctgg cagaccgggg cgtatgtttt ctgttttgtc gaccgggccc agcatctggc
    17941 gatgatgaaa tccatcggga tgtatgacga gcggctcaac agcgacgaaa aagccacgta
    18001 ctttttcacc ggtaaaaatc cgcaatggga acgggacaaa cccgcaggaa tgaagctgtg
    18061 tgattacctc tatcgcaagg tggagcggtt cgccatcgag aacggtgtgc aggtggttga
    18121 tgccgggaag tgctcaccgg agcagattag cgcgatgatt gaggtggtca gcgagcggca
    18181 cggggaaagc gcggtgaacg agaagctgaa agcggagctg atgcgggaat ataacagccg
    18241 ctattttaac gtgccggtgc ctgaactgcc ggaggacaaa cccgcccgcc ggtggtggcc
    18301 gttcggcaaa aaataaacgg cagcaccggc catgccggtg ctgttattcc ttcccgagct
    18361 tatcccgcat cacctgccag agtgtttccg catcctcgtg cagccgctcg cacatgtcgg
    18421 ccggggtatc catatccagc tcgttcagtt tctcgagtaa cagcagcgac tggtccttaa
    18481 taaatttagc catattgagg gcagtttttt cctgcttagt catgttttct gtccttccgg
    18541 gtgtggtgtg cttcctgtcg ggtatactgt ctcaaaaccg gaggtgagac aatggcgtgg
    18601 gtgacggtga gacaggcgac ggaactgacg gggaaagccc ggagttccct gtatagggat
    18661 atggcgaaag gccgcgtatc ataccgtact gaagctgatg gtggtcgggt ggtggatacc
    18721 agcgaactta tccgggtgta cggtgaatta agacggattg agactcacga gcgggacggg
    18781 ttgagactgt cggatgagac tgaaaaaatg gcctccgacg cgttgatcgc ggagataaaa
    18841 gcactcaggg aggaggtggc cggactgcgg caggaaatgc aggccatgag actgctggaa
    18901 cacaaaccag agacagcaga gacaccggcc ggaacatcgc gccgatggtg gcagtgggga
    18961 aaacgctgac gcgctgtgct tgtccaaccg gcgacggcca cgcaatgaat tgcgccccca
    19021 tcaccggtta tcagcgaacg gtatggggat tctggcgctg gccaaccacg gccagaaccc
    19081 ggactttcct ggtctggtca tcaacatcat agaggacatg ctgtcccatc agactgatcc
    19141 ggcgaacgtc ctcgccatag cgtgagtcca cttcatatag gcgcgggaag tcggccagtt
    19201 tgtccgctgc agcgagaact cgatcggtaa cgttctgcgc atcccacagg gatgaatttt
    19261 ccgcaatgtg gtcagacgat tgctgtcaca tcctcgatgg ctccctgctt ccattccacc
    19321 tcatacggca tcacgtagtt cctgatagcg gccgtgccgt ctctttggcg gagttgttcc
    19381 gtggtcatac ccgggcgcgt gtcagcgcgg gcggcgggtc actttagcgc aaacctgatc
    19441 ttcatattct gcctgggcca gtt
//
