LOCUS       NZ_CP141567            18587 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP44_NW4_44.22_ST307_OXA48 plasmid
            unnamed2, complete sequence.
ACCESSION   NZ_CP141567
VERSION     NZ_CP141567.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38771712
            Assembly: GCF_035597335.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 18587)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18587)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAR-2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Assembly Name                     :: q20run9_BC05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 53x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 11:58:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,574
            CDSs (total)                      :: 5,450
            Genes (coding)                    :: 5,290
            CDSs (with protein)               :: 5,290
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 160
            CDSs (without protein)            :: 160
            Pseudo Genes (ambiguous residues) :: 0 of 160
            Pseudo Genes (frameshifted)       :: 80 of 160
            Pseudo Genes (incomplete)         :: 81 of 160
            Pseudo Genes (internal stop)      :: 16 of 160
            Pseudo Genes (multiple problems)  :: 15 of 160
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141567.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..18587
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP44_NW4_44.22_ST307_OXA48"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-09-08"
                     /genotype="ST307"
     gene            complement(join(18284..18587,1..227))
                     /locus_tag="VI610_RS27765"
                     /old_locus_tag="VI610_27770"
     CDS             complement(join(18284..18587,1..227))
                     /locus_tag="VI610_RS27765"
                     /old_locus_tag="VI610_27770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_324670471.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKAAYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(234..965)
                     /locus_tag="VI610_RS27770"
                     /old_locus_tag="VI610_27775"
     CDS             complement(234..965)
                     /locus_tag="VI610_RS27770"
                     /old_locus_tag="VI610_27775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(965..2929)
                     /locus_tag="VI610_RS27775"
                     /old_locus_tag="VI610_27780"
     CDS             complement(965..2929)
                     /locus_tag="VI610_RS27775"
                     /old_locus_tag="VI610_27780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4411..4560)
                     /locus_tag="VI610_RS27780"
                     /old_locus_tag="VI610_27785"
     CDS             complement(4411..4560)
                     /locus_tag="VI610_RS27780"
                     /old_locus_tag="VI610_27785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4645..4902)
                     /locus_tag="VI610_RS27785"
                     /old_locus_tag="VI610_27790"
     CDS             complement(4645..4902)
                     /locus_tag="VI610_RS27785"
                     /old_locus_tag="VI610_27790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(4912..6597)
                     /locus_tag="VI610_RS27790"
                     /old_locus_tag="VI610_27795"
     CDS             complement(4912..6597)
                     /locus_tag="VI610_RS27790"
                     /old_locus_tag="VI610_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            6999..7418
                     /locus_tag="VI610_RS27795"
                     /old_locus_tag="VI610_27800"
     CDS             6999..7418
                     /locus_tag="VI610_RS27795"
                     /old_locus_tag="VI610_27800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            7724..7987
                     /locus_tag="VI610_RS27800"
                     /old_locus_tag="VI610_27805"
     CDS             7724..7987
                     /locus_tag="VI610_RS27800"
                     /old_locus_tag="VI610_27805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            7977..8276
                     /locus_tag="VI610_RS27805"
                     /old_locus_tag="VI610_27810"
     CDS             7977..8276
                     /locus_tag="VI610_RS27805"
                     /old_locus_tag="VI610_27810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8338..8715)
                     /locus_tag="VI610_RS27810"
                     /old_locus_tag="VI610_27815"
     CDS             complement(8338..8715)
                     /locus_tag="VI610_RS27810"
                     /old_locus_tag="VI610_27815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            8784..8963
                     /locus_tag="VI610_RS27815"
                     /old_locus_tag="VI610_27820"
     CDS             8784..8963
                     /locus_tag="VI610_RS27815"
                     /old_locus_tag="VI610_27820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8991..9521)
                     /locus_tag="VI610_RS27820"
                     /old_locus_tag="VI610_27825"
     CDS             complement(8991..9521)
                     /locus_tag="VI610_RS27820"
                     /old_locus_tag="VI610_27825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004153058.1"
                     /translation="MALYMVFIEDAEDRNGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERNKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIDVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(9528..10259)
                     /locus_tag="VI610_RS27825"
                     /old_locus_tag="VI610_27830"
     CDS             complement(9528..10259)
                     /locus_tag="VI610_RS27825"
                     /old_locus_tag="VI610_27830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_004152551.1"
                     /translation="MALELYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRTKYPVDGRQVSVWGLTPHGVAFSFDEDEPLADVIPFQP
                     SRVSAAHLPHRLAVQSLRLDMEARGATGWRILHRLSLKGMKVPDALAELDGKTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="MobC family replication-relaxation protein"
     gene            complement(10259..12223)
                     /locus_tag="VI610_RS27830"
                     /old_locus_tag="VI610_27835"
     CDS             complement(10259..12223)
                     /locus_tag="VI610_RS27830"
                     /old_locus_tag="VI610_27835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_004178196.1"
                     /translation="MFNTDSLAWKWHIPELARQIYPWVSWLYQEYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNKRPAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLA
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSSEQARQEQAVFLGKDEQGKPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLI
                     AGLNLGETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     RLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFREWPHVSVMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASLESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEF
                     RPPADGGGPKADDDFAGIY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(13704..13853)
                     /locus_tag="VI610_RS27835"
                     /old_locus_tag="VI610_27840"
     CDS             complement(13704..13853)
                     /locus_tag="VI610_RS27835"
                     /old_locus_tag="VI610_27840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_004152552.1"
                     /translation="MKKVKVVFLFILIFSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(13938..14195)
                     /locus_tag="VI610_RS27840"
                     /old_locus_tag="VI610_27845"
     CDS             complement(13938..14195)
                     /locus_tag="VI610_RS27840"
                     /old_locus_tag="VI610_27845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_004152553.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKSWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(14205..15890)
                     /locus_tag="VI610_RS27845"
                     /old_locus_tag="VI610_27850"
     CDS             complement(14205..15890)
                     /locus_tag="VI610_RS27845"
                     /old_locus_tag="VI610_27850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_004178188.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IVTSLPADTVTETPVSSLPLDQATVSVTKRVADIVKDERQHIAVVTGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPAAKAPPKGIIAEKGDSRPAGFTAGGNSREAVIR
                     FPKESGQKPVYVSVTDVLTPAQVKQRLEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNIATLNSRIASTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQTSLNEMNESLSRDKAALSGAMESRKQKEKKAKDAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            16292..16711
                     /locus_tag="VI610_RS27850"
                     /old_locus_tag="VI610_27855"
     CDS             16292..16711
                     /locus_tag="VI610_RS27850"
                     /old_locus_tag="VI610_27855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254601.1"
                     /translation="MRRSGSKLCLIAMITATATLTACAPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLQGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            17017..17280
                     /locus_tag="VI610_RS27855"
                     /old_locus_tag="VI610_27860"
     CDS             17017..17280
                     /locus_tag="VI610_RS27855"
                     /old_locus_tag="VI610_27860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000072676.1"
                     /translation="MSNTTHYENANFLRELAESLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            17270..17569
                     /locus_tag="VI610_RS27860"
                     /old_locus_tag="VI610_27865"
     CDS             17270..17569
                     /locus_tag="VI610_RS27860"
                     /old_locus_tag="VI610_27865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146176.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_001146176.1"
                     /translation="MPYDVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHIVL"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(17631..18008)
                     /locus_tag="VI610_RS27865"
                     /old_locus_tag="VI610_27870"
     CDS             complement(17631..18008)
                     /locus_tag="VI610_RS27865"
                     /old_locus_tag="VI610_27870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000312627.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQDETHERDELRLSGETEKMASDALIAEIKALREEVAGLRQEMQSMRLLEH
                     KPETAETPAGTPRRWWPWGKR"
                     /name="hypothetical protein"
     gene            18077..18256
                     /locus_tag="VI610_RS27870"
                     /old_locus_tag="VI610_27875"
     CDS             18077..18256
                     /locus_tag="VI610_RS27870"
                     /old_locus_tag="VI610_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165971.1"
                     /translation="MTKQEKTALNMAKFIKEQSLLLLDKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             1..18587
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3730..4308
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          13023..13601
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.361"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(238..965)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.35e-150"
     Typing          complement(9532..10259)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.35e-150"
ORIGIN
        1 ccagtgaaaa agtacgcggc tttttcgtcg ctgttgagcc gctcgtcata catcccgatg
       61 gatttcatca tcgccagatg ctgggcccgg tcgacaaaac agaaaacata cgccccggtc
      121 tgccaggcga gcaccaccat atcttccaga tgctcgctgg tcggcatgat gcagcgctcg
      181 ccgttccggt cctccgcgtc ttcaataaat accatgtaca gcgccataat ccctcactcc
      241 gttgtcataa atttaaagca cgcaaacaac cgttcacgat ccagctctgc cgttttgcgg
      301 gcctcgccgg tctgtgggtt cacgatttca tccaccgaca ggattgcccg ctgcaccctg
      361 acctgcgtcg cccggtccgg gcagatgtac cagatttcat cgatgccgtt agctttgcgg
      421 ttgaacagat acccggacac cacttcctga taccggcgac ggctcttgac ggtgcgctcc
      481 acctcaaacg ccaccgtttt accgtccagc tccgccaggg cgtccggcac tttcatgcct
      541 ttaagactca gacggtgcag aatgcgccag ccggtggccc ctctggcttc catatcaagg
      601 cgcagggatt gcacggccag ccggtgcggc agatgtgctg ccgacaccct tgacggctga
      661 aacggaataa catccgccag cggttcgtcc tcgtcgaacg agaacgccac gccatgcggc
      721 gtcagccccc acacgctgac ctgacggccg tccaccggat atttggtccg gaccagcagc
      781 cctttcgcca ccaggctgtc gaggaagcgg tagaccggct gcaccgccgc aatccccagc
      841 cgccgcgcaa ccagctccgc cgtggaacac agctcttccc gcaaccagcg cagggtcagc
      901 cgggttttct ccgcgttccg cgcctgtcgt ttcgcgtggc tgacgttgta cagctccagc
      961 gccatcagta aatccccgca aaatcatcat ccgctttcgg cccgccgccg tcggccgggg
     1021 gccggaactc cgccagggga tcggttttcg ccagcaccgg gaaacgctgc aacgggattt
     1081 cccggcgctc aacccgcagc ggactgctga acgccatccg ggccacaccc agcccggtca
     1141 tgaccgcgaa cccgtccggc aggtgttgca gggtgttcac gtcaaacagc ggccgcgcct
     1201 ccttctggat gaagctgtcc ccgctgatat gctccacgtt ccccgcctcc cggctggcac
     1261 ggcggcgctc cacgtccacc ctgatttccc cggtctgccc cgccgcccac tgcgcggact
     1321 ccaggctggc cgcccggtaa aaccagcgga tcggcgtgtt atccagtacc gtggttttca
     1381 caaattcagc gggcagatcc tgcccgcact gcccgaaatc gcccagcgac tggtgcgcga
     1441 gcaacagatt gcagtcccgg ctgcgcagcg tccccagtgc gttcagtaca tacttcgaca
     1501 gcagatattt aatttcatcg agcatcacgc tgacgtgtgg ccactcccgg aactcgtccc
     1561 gcgcaatgat gatttgcgcg cagcgggcaa acagcatttt ttgcacacgg atcaccgcct
     1621 cgtcgtccat cgagccgata acgtacaggc agccgccgcc gttgataatc cccgccacat
     1681 cgataccact gtccgtctgc aggacgctca ggtcagcgac gttttccagc ctagtgatca
     1741 accccttcac gtcctttttc agggcctccg gcagcgcgta cgccgccgcc agcacctgct
     1801 gaatgtttgc cccctgcgga aactgctccg caatcagctt cgccgctttc tgctcgctga
     1861 tgcggtagtg gtccgccacg tcgccggtct cgcccaggtt aagcccggca atcagcaggt
     1921 ttttcagcgc gtactggtcg agatcgcgga acagattcag ctgcgccggt ttccccggcc
     1981 gcagatccag cagggtgaag tccggacagt gcgcccggaa gacgtgcggc gcccatgcgt
     2041 cccctttggg gtcgaagtac accaccgcgt ccccgaaatc gcgtacgcag cgaatgagcg
     2101 cgttggtggc catcacgctc ttgccgctcc cgggcaggcc gaggatctga atgttggtct
     2161 tgcgccaggt atcacgcgga accagcaccg gcttgccctg ctcatcctta cccagaaaca
     2221 ccgcctgctc ctgccgggcc tgctccgagg aaaaatcggc cagcgcatta accggtaccg
     2281 gctccccgaa atgcaggtgg cgcacgtcgg tcatctctcc tgtccgctgg ctgctgcgcc
     2341 ggctctcctg attcagtttc gcgttcaccg cctcgtcagc agccgcaaaa tgaccgtcaa
     2401 gcagcaggaa cgccagcagc cgcgccaaca caatcccgac agccagcccg cccgcgcacc
     2461 aggcaatccc gaacaggtta gccggctgca gcaggtaata atctgcgttt gcgtagaggt
     2521 ccggacgcag gacggtttct ccccagctct cgataaacac ggcgacaacc agccccccca
     2581 gaagggccag accggccgcg ccggtcagcc agtacgcgta gttccagaca tgtcgacgtg
     2641 ccctgaccat ccgcccgggc gaatccagcg aggccagcac cgaaaatggc gctggccgtt
     2701 tattatcgcg cttatagcga tcggcatact cccgtcgctc cttatgactc agccccacaa
     2761 aacgcatccg tccggcccaa agttcagcca gaccgaaaaa caggccgata actaggaaag
     2821 ccccggccag accgtaatac tcctggtaca gccagctgac ccacggataa atctgccggg
     2881 ccagctccgg aatgtgccac ttccaggcaa gcgaatccgt attaaacatg ttctctctcc
     2941 ttcagacgat ggtttgccgg cgtgatgtgc agcccccgct gcacaagccg cggacatgcc
     3001 ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg ttcagctttc accacggagg
     3061 ccagcctctt tccgccccat gcggaaaagg cgcagccccg gaggaacaac gagcacgccc
     3121 cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt ttccggtgtt ccggcccccg
     3181 tcagagttcg ccatagcgag cggctgacca cccgcaaaat caggagcagc tttgctgctt
     3241 cgccaggtgc gtccagcacc gtggattttt gtgggtggtc aggcgtgagc ctgacggggg
     3301 ccgggtgaat ttctggcgtg gccggcgtcc agccggacgg agccagaaag agggggagag
     3361 accgtgcgtc ggtggcgtcc agccagcgta agcaggggat atcccccctg aggtcagcca
     3421 cgccctcgcg gggatgacct gtatttctcc gtaaggagaa aaaagcaaca gagataggtt
     3481 aacgtaaaat gttcagcgaa aaaccgatat gattcaccta tcctggtaat ttattatcag
     3541 ggaatataaa gacttaatca ggggacaata tgccacatca gcgcgtccgg tgtccgtctt
     3601 gtaggtgccg gggaaattgc gcctcctcgc tcactcggtc gctacgctcc ggccgtgaga
     3661 ctgcggcggg cgctgcggac acatgcaaag ttacccacag attccgtgaa taagcagggg
     3721 actaacatgt gaggcaaaac ggcaaggcca cgcaggtggc gtttttccat aggctccgcc
     3781 ctcctgactg agttcacata aacagacgct ttttcggtgc atctgtggga gccggtggct
     3841 caaccatgaa tcagtcagga gtggcgaaac ccgacaggac ttaaagatca tcaccgtttc
     3901 cgatgaatcg ctccctcttg cgctctcctg ttccaaccct gccgtttccc ggatacctgt
     3961 tccgcctttc tcccttgcgg gaagtgtggc gctttctcat agctcacact ctggtatctc
     4021 ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc aagaaccccc cgttcagccc
     4081 gactgctgcg ccttatccgg taactgttca cttgagtcca acccggaaaa gcacgcaaaa
     4141 cgccactggc agcagccatt ggtaactggg agttcgcaga ggatttgttt agctaaacac
     4201 gcggttgctc ttgaagtgtg cgccaaagtc cggctacact ggaaggacag atttggttgc
     4261 tgtgctctgc gaaagccagt taccacggtt aagcagttcc ccaactgact taaccttcga
     4321 tcaaaccacc tccccaggtg gttttttcgt ttacagggca aaagattacg cgcagaaaaa
     4381 aaggatctca agaagatcct ttgatctttt ctactgaacc gcgatccccg tcagttcaga
     4441 ggaggacgat ggtgccaccg ttccaccctg aacatcccgg atatagtttg cctgacatgc
     4501 gaccagaaga aagcctgaaa aaatcaatat aaaaagaaaa acaaccttta cctttttcat
     4561 ataccttccc tgtatttaaa aaccccggca ttcatccctg aacaccggaa gtctgtattt
     4621 caaatcatcc ttgataactt ttgcttacca gttaccatcc cggtaaacaa aggatacaaa
     4681 gtaatcaaat ttactaatat cgattacatt tttaaagtgt ggctgtaata tcgaaaccca
     4741 tgatttttca acatcaaacc aaccattatt aatattatcc tttaaaggca tccccagact
     4801 ttcaatgaca gaaccatcat caccgaagtc ttttgagtat tcaccacctt taaactcttc
     4861 ggttttctta tcaaaccaat gaatatgtaa tttaagcccc atatttacct cttaaagata
     4921 ttttttaatg tttcgttttg gatccggccc tttaacctgc ttacccgttt ttggatcgaa
     4981 tgccccgagg tgttcgccat cactggcgcg gtacccttca agctcaccgt gctgggaatc
     5041 ccattcataa attttacgct ttttatcacc ataccatcgg gcccgcttac caccaccacc
     5101 ttgtttaggg gttttaggtt taccctcttt caactctccc aaccccttaa tatcttcagt
     5161 cttgggatca ggaaaataat catggccgta atctttagtt cccttacgag gttttttctt
     5221 ttcctcattt aatttatttt ctgcatcctt cgcttttttc tccttttgtt tccggctctc
     5281 catcgccccg gaaagcgcag ccttatccct ggataaggat tcattcattt cgtttaaaga
     5341 tgtctgggca gcttttaatt tctcattctg attctgaata tccttatcaa gatagcgaac
     5401 ctgatcctgc cacccggagc catattcatg aggcgggtta taagtcacaa aatcatcttt
     5461 atttgcctct gcctctttta cctttttatc ggcttcctga acagcagccc ttgcaccggg
     5521 gatcgccttc tctgtcgatg caatgcgact gtttaaggtc gcaatatttt tatcttcagc
     5581 atccagctca gctttcgctt tatcatactc tcttttcagc ccctcttccg ggtgagcagc
     5641 gtcccatgcc tgctggcgac gcttttcttc ctccagacgc tgttttacct gtgccggagt
     5701 aagaacatcc gtcaccgaca catagaccgg tttctgtccg ctctctttcg ggaaacgaat
     5761 aacggcctca cgggagttac caccggccgt aaaaccagcc ggacgtgaat cacctttttc
     5821 agcaataatg ccttttggcg gggctttcgc ggccggaaca cctttaggta cgctcacctg
     5881 cagagacggg agacccggaa tcgacacact gaataccccc ggacgttttg tcggtttcgc
     5941 atccaccaca ggaacactca ttggccggcc ggtgacaacc gcaatatgct gccgctcgtc
     6001 cttcacaata tccgccacac gtttagtgac gctgaccgtc gcctggtcca gcggcaggga
     6061 gctgaccggc gtctctgtta ccgtgtctgc cggcaatgac gtcacgattt ttgacatcat
     6121 tttcggatca tcttttgcta tctcagaagg cagcactccg taaatcgcaa tcccccacag
     6181 accaaactta aacggacctt tcagggcagc cagcacatca gcaacagcgg ccgacaacgc
     6241 atcgccggaa acggataaag ccagtccttc agcccccgga gtggccaggg caggcagacc
     6301 gaaggccatc gcggtggccg aagactgtcc gccaccagag ctcccgctat gattgccgct
     6361 gttaccgcca ttaccaccgg gtttactccc gccgttacca aactcccccg ggttatagtt
     6421 atgaatatcc ccgttaggtg tatgcgtcgt accccagccc ggcccgctgt ttggatccgt
     6481 accaccacct gatgaagggc cacctttacc tgatgtaccg ttcacattcc cactggcctg
     6541 accaccattg tgtcccagac ctgaattacc cggacctcgt ccgtctccgc cactcatata
     6601 cttttcctct ttgactttta aaacaataaa ctaaaataaa atactgtaca tataaccact
     6661 ggttttatat acagtataaa aattacgctg ccttgttttt catgtcaagt ctgtaaattt
     6721 gcgtttttgt gaaaatgatc aaaaaaacag ccactcacac catgcgcaat cccctccaga
     6781 tgcaacaaaa accagcacaa acggcgccgt tctccggcct cagtatgttt tactgaaatc
     6841 attatatgag ataccagaaa gccccctccg ggacaggaaa agaaacgggg gtctggttcg
     6901 ggttgttcgg tcactgaaca cggtcagaaa ctgatgataa gatagcctgc ggatatataa
     6961 ctcaatgaga aaaaagaatt aaaggtaagg agacgctcat gagacgttcc ggcagcaaac
     7021 tgtgtcttat cgccatgata acagcgacag caactctcac tgcctgtgct ccaaagggca
     7081 gcgtggaaca gcatacccgg cattacgtat atgcttcaga tgacggtttt gatcccaatt
     7141 tttccaccca gaaagctgac acaacacgaa tgatggtgcc ttttttccgg cagttctggg
     7201 atatgggcgc aaaggataaa gcgaccggaa aatcacggag tgatgtgcag caacgcattc
     7261 agcagtttca cagtcaggaa tttttaaact cactccaagg cacaacacaa tttgcgggta
     7321 ccgattaccg cagcaaagac ctcacaccga aaaaatcaag gctactggct gacaccattt
     7381 cagctgttta tctcgatggc tatgaaggca gacagtaagt ggatttacca taacgcgtca
     7441 ttacgcgcac cgctcagacg ggctcagggc gtttccggcg gctacgggat aaaacaggca
     7501 acaacccccc cggaaaactg ccggaaacgc gcccgataaa ttttatccta atgcatgcct
     7561 atgcacgcaa ctgcatggca tatgttttga cgtaaaatgg atgccgtaag ccctttttta
     7621 ccgactttct gcttcatcag cctgactgac acgaccatat tctttcagac cgccccctgc
     7681 taaacttcct tcatattgcc gcattaaccc gggaggctcc gcaatgagca acactacaca
     7741 ctacgaaaac gccaactttc ttcgggaact ggctgaaagc ctgccccgca tactgccgga
     7801 aggtggcccg gacaaagctg cgctgttgca gcgactggct aacgaagaac tggcccaggc
     7861 agagtatgaa gatcaggttc gcgccaaagt gaccgccgcc cgtgctgaca cgcgtccggg
     7921 tatgaccacg gaacaactcc gccagcgcct gcatggccgt tatcaggaac tgcgcgatgc
     7981 cgtatgacgt ggaatggaag cagggagcca tcgaggatgt aacgacgctg tttgactata
     8041 tcgcggaaaa ttcatccctg tgggatgcgc agaacgttac cgaccgggtt ctcgctgcgg
     8101 cggacaaact ggccgacttc ccgcgactct atgaagtgga ctcacgctat ggcgaggacg
     8161 ttcgccggat cagtctgatg ggacagcatg tcctctatga tgttgatgac cagaccagaa
     8221 aagtccgggt tctggccgtg gtcggccagc gccagaatcc ccatattgtt ctctaataac
     8281 cggtgacggg gacgcaattc attgcgtggc cgtcgccggt tggacaagca cagcgcgtca
     8341 gcgttttccc cacggccacc accggcgcgg tgttccggcc ggggtctctg ctgtctccgg
     8401 tttgtgttcc agcagtctca tggactgcat ttcctgccgt agcccggcca cctcctccct
     8461 gagtgctttt atctccgcga tcaacgcgtc ggaggccatt ttttcagtct cacccgacag
     8521 tctcaactcg tcccgctcgt gagtctcatc ctgtcttaac tcaccgtaca cccggataag
     8581 ctcgctggta tccaccaccc ggccgccatc agcttccgta cggtatgata cgcggccttt
     8641 cgccatatcc cgatacaggg aacttcgggc tttacccgtc agttccgtcg cctgtctcac
     8701 cgtcacccac gccattgtct caccccgcta tttttgagac agtatacctg acaggcgcac
     8761 accatgcccg gaaggaaaga atcatgacaa aacaggaaaa aacagccctc aatatggcta
     8821 aatttattaa ggagcagtcg ctgctattgc tcgataaact gaacgagctg gatatggata
     8881 ccccggccga catgtgcgag cggctacacg aggatgcgga aacactctgg caggtgatgc
     8941 gggataagct cggggaggaa taacagcacc ggcatggccg gtgctggcat ttattttttg
     9001 ccgaacggcc accaccggcg ggcaggtttg tcctccggca gttcaggtac cggcacgtta
     9061 aaatagcggc tgttatattc ccgcatcagc tccgctttca gcttctcgtt caccgcgctt
     9121 tccccgtgcc gctcactcac cacgtcaatc atcgcgttaa tctgctccgg tgagcacttc
     9181 ccggcatcaa ccacctgcac accgttctcg atggcgaacc gctccacctt gcgatagagg
     9241 taatcacaca gcttcattcc tgtgggtttg ttccgttccc attgcggatt tttaccagtg
     9301 aaaaagtacg tggctttttc gtcgctgttg agccgctcgt catacatccc gatggatttc
     9361 atcatcgcca gatgctgggc ccggtcgaca aaacagaaaa catacgcccc ggtctgccag
     9421 gcgagcacca ccatatcttc cagatgctcg ctggtcggca tgatgcagcg ctcgccgttc
     9481 cggtcctccg cgtcttcaat aaataccatg tacagcgcca taatccctca ctccgttgtc
     9541 ataaatttaa agcacgcaaa caaccgttca cgatccagct ctgccgtttt gcgggcctcg
     9601 ccggtctgtg ggttcacgat ttcatccacc gacaggattg cccgctgcac cctgacctgc
     9661 gtcgcccggt ccgggcagat gtaccagatt tcatcgatgc cgttagcttt gcggttgaac
     9721 agatacccgg acaccacttc ctgataccgg cgacggctct tgacggtgcg ctccacctca
     9781 aacgccaccg ttttaccgtc cagctccgcc agggcgtccg gcactttcat gcctttaaga
     9841 ctcagacggt gcagaatgcg ccagccggtg gcccctctgg cttccatatc aaggcgcagg
     9901 gattgcacgg ccagccggtg cggcagatgt gctgccgaca cccttgacgg ctgaaacgga
     9961 ataacatccg ccagcggttc gtcctcgtcg aacgagaacg ccacgccatg cggcgtcagc
    10021 ccccacacgc tgacctgacg gccgtccacc ggatatttgg tccggaccag cagccctttc
    10081 gccaccaggc tgtcgaggaa gcggtagacc ggctgcaccg ccgcaatccc cagccgccgc
    10141 gcaaccagct ccgccgtgga acacagctct tcccgcaacc agcgcagggt cagccgggtt
    10201 ttctccgcgt tccgcgcctg tcgtttcgcg tggctgacgt tgtacagctc cagcgccatc
    10261 agtaaatccc cgcaaaatca tcatccgctt tcggcccgcc gccgtcggcc gggggccgga
    10321 actccgccag gggatcggtt ttcgccagca ccgggaaacg ctgcaacggg atttcccggc
    10381 gctcaacccg cagcggactg ctgaacgcca tccgggccac acccagcccg gtcatgaccg
    10441 cgaacccgtc cggcaggtgt tgcagggtgt tcacgtcaaa cagcggccgc gcctccttct
    10501 ggatgaagct gtccccgctg atatgctcca cgttccccgc ctcccggctg gcacggcggc
    10561 gctccacgtc caccctgatt tccccggtct gccccgccgc ccactgcgcg gactccaggc
    10621 tggccgcccg gtaaaaccag cggatcggcg tgttatccag taccgtggtt ttcacaaatt
    10681 cagcgggcag atcctgcccg cactgcccga aatcgcccag cgactggtgc gcgagcaaca
    10741 gattgcagtc ccggctgcgc agcgtcccca gtgcgttcag tacatacttc gacagcagat
    10801 atttaatttc atcgagcatc acgctgacgt gtggccactc ccggaactcg tcccgcgcaa
    10861 tgatgatttg cgcgcagcgg gcaaacagca ttttttgcac acggatcacc gcctcgtcgt
    10921 ccatcgagcc gataacgtac aggcagccgc cgccgttgat aatccccgcc acatcgatac
    10981 cactgtccgt ctgcaggacg ctcaggtcag cgacgttttc cagcctagtg atcaacccct
    11041 tcacgtcctt tttcagggcc tccggcagcg cgtacgccgc cgccagcacc tgctgaatgt
    11101 ttgccccctg cggaaactgc tccgcaatca gcttcgccgc tttctgctcg ctgatgcggt
    11161 agtggtccgc cacgtcgccg gtctcgccca ggttaagccc ggcaatcagc aggtttttca
    11221 gcgcgtactg gtcgagatcg cggaacagat tcagctgcgc cggtttcccc ggccgcagat
    11281 ccagcagggt gaagtccgga cagtgcgccc ggaagacgtg cggcgcccat gcgtcccctt
    11341 tggggtcgaa gtacaccacc gcgtccccga aatcgcgtac gcagcgaatg agcgcgttgg
    11401 tggccatcac gctcttgccg ctcccgggca ggccgaggat ctgaatgttg gtcttgcgcc
    11461 aggtatcacg cggaaccagc accggcttgc cctgctcatc cttacccaga aacaccgcct
    11521 gctcctgccg ggcctgctcc gaggaaaaat cggccagcgc attaaccggt accggctccc
    11581 cgaaatgcag gtggcgcacg tcggtcatct ctcctgtccg ctggctgctg cgccggctct
    11641 cctgattcag tttcgcgttc accgcctcgt cagcagccgc aaaatgaccg tcaagcagca
    11701 ggaacgccag cagccgcgcc aacacaatcc cgacagccag cccgcccgcg caccaggcaa
    11761 tcccgaacag gttagccggc tgcagcaggt aataatctgc gtttgcgtag aggtccggac
    11821 gcaggacggt ttctccccag ctctcgataa acacggcgac aaccagcccc cccagaaggg
    11881 ccagaccggc cgcgccggtc agccagtacg cgtagttcca gacatgtcga cgtgccctga
    11941 ccatccgccc gggcgaatcc agcgaggcca gcaccgaaaa tggcgctggc cgtttattat
    12001 cgcgcttata gcgatcggca tactcccgtc gctccttatg actcagcccc acaaaacgca
    12061 tccgtccggc ccaaagttca gccagaccga aaaacaggcc gataactagg aaagccccgg
    12121 ccagaccgta atactcctgg tacagccagc tgacccacgg ataaatctgc cgggccagct
    12181 ccggaatgtg ccacttccag gcaagcgaat ccgtattaaa catgttctct ctccttcaga
    12241 cgatggtttg ccggcgtgat gtgcagcccc cgctgcacaa gccgcggaca tgccccaagg
    12301 catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccacg gaggccagcc
    12361 tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac gccccagcgt
    12421 gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc cccgtcagag
    12481 ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct gcttcgccag
    12541 gtgcgtccag caccgtggat ttttgtgggt ggtcaggcgt gagcctgacg ggggccgggt
    12601 gaatttctgg cgtggccggc gtccagccgg acggagccag aaagaggggg agagaccgtg
    12661 cgtcggtggc gtccagccag cgtaagcagg ggatatcccc ctgaggtcag ccacgccctc
    12721 gcggggatga cctgtatttc tccgtaagga gaaaaaagca acagagatag gttaacgtaa
    12781 aatgttcagc gaaaaaccga tatgattcac ctatcctggt aatttattat cagggaatat
    12841 aaagacttaa tcaggggaca atatgccaca tcagcgcgtc cggtgtccgt cttgtaggtg
    12901 ccggggaaat tgcgcctcct cgctcactcg gtcgctacgc tccggccgtg agactgcggc
    12961 gggcgctgcg gacacatgca aagttaccca cagattccgt gaataagcag gggactaaca
    13021 tgtgaggcaa aacggcaagg ccacgcaggt ggcgtttttc cataggctcc gccctcctga
    13081 ctgagttcac ataaacagac gctttttcgg tgcatctgtg ggagccggtg gctcaaccat
    13141 gaatcagtca ggagtggcga aacccgacag gacttaaaga tcatcaccgt ttccgatgaa
    13201 tcgctccctc ttgcgctctc ctgttccaac cctgccgttt cccggatacc tgttccgcct
    13261 ttctcccttg cgggaagtgt ggcgctttct catagctcac actctggtat ctcggctcgg
    13321 tgtaggtcgt tcgctccaag ctgggctgta agcaagaacc ccccgttcag cccgactgct
    13381 gcgccttatc cggtaactgt tcacttgagt ccaacccgga aaagcacgca aaacgccact
    13441 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
    13501 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
    13561 tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc
    13621 acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
    13681 tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc agaggaggac
    13741 gatggtgcca ccgttccacc ctgaacatcc cggatatagt ttgcctgaca tgcgaccaga
    13801 agaaagcctg aaaaaatcaa tataaaaaga aaaacaacct ttaccttttt catatacctt
    13861 ccctgtattt aaaaaccccg gcattcatcc ctgaacaccg gaagtctgta tttcaaatca
    13921 tccttgataa cttttgctta ccagttacca tcccggtaaa caaaggatac aaagtaatca
    13981 aatttactaa tatcgattac atttttaaag tgtggctgta atatcgaaac ccatgatttt
    14041 tcaacatcaa accaaccatt attaatatta tcctttaaag gcatccccag actttcaatg
    14101 acagaaccat catcaccgaa gtcttttgag tattcaccac ctttaaactc ttcggttttc
    14161 ttatcaaacc aatgaatatg taatttaagc cccatattta cctcttaaag atatttttta
    14221 atgtttcgtt ttggatccgg ccctttaacc tgcttacccg tttttggatc gaatgccccg
    14281 aggtgttcgc catcactggc gcggtaccct tcaagctcac cgtgctggga atcccattca
    14341 taaattttac gctttttatc accataccat cgggcccgct taccaccacc accttgttta
    14401 ggggttttag gtttaccctc tttcaactct cccaacccct taatatcttc agtcttggga
    14461 tcaggaaaat aatcatggcc gtaatcttta gttcccttac gaggtttttt cttttcctca
    14521 tttaatttat tttctgcatc cttcgctttt ttctcctttt gtttccggct ctccatcgcc
    14581 ccggaaagcg cagccttatc cctggataag gattcattca tttcgtttaa agatgtctgg
    14641 gcagctttta atttctcatt ctgattctga atatccttat caagatagcg aacctgatcc
    14701 tgccacccgg agccatattc atgaggcggg ttataagtca caaaatcatc tttatttgcc
    14761 tctgcctctt ttaccttttt atcggcttcc tgaacagcag cccttgcacc ggggatcgcc
    14821 ttctctgtcg atgcaatgcg actgtttaag gtcgcaatat ttttatcttc agcatccagc
    14881 tcagctttcg ctttatcata ctctcttttc agcccctctt ccgggtgagc agcgtcccat
    14941 gcctgctggc gacgcttttc ttcctccaga cgctgtttta cctgtgccgg agtaagaaca
    15001 tccgtcaccg acacatagac cggtttctgt ccgctctctt tcgggaaacg aataacggcc
    15061 tcacgggagt taccaccggc cgtaaaacca gccggacgtg aatcaccttt ttcagcaata
    15121 atgccttttg gcggggcttt cgcggccgga acacctttag gtacgctcac ctgcagagac
    15181 gggagacccg gaatcgacac actgaatacc cccggacgtt ttgtcggttt cgcatccacc
    15241 acaggaacac tcattggccg gccggtgaca accgcaatat gctgccgctc gtccttcaca
    15301 atatccgcca cacgtttagt gacgctgacc gtcgcctggt ccagcggcag ggagctgacc
    15361 ggcgtctctg ttaccgtgtc tgccggcaat gacgtcacga tttttgacat cattttcgga
    15421 tcatcttttg ctatctcaga aggcagcact ccgtaaatcg caatccccca cagaccaaac
    15481 ttaaacggac ctttcagggc agccagcaca tcagcaacag cggccgacaa cgcatcgccg
    15541 gaaacggata aagccagtcc ttcagccccc ggagtggcca gggcaggcag accgaaggcc
    15601 atcgcggtgg ccgaagactg tccgccacca gagctcccgc tatgattgcc gctgttaccg
    15661 ccattaccac cgggtttact cccgccgtta ccaaactccc ccgggttata gttatgaata
    15721 tccccgttag gtgtatgcgt cgtaccccag cccggcccgc tgtttggatc cgtaccacca
    15781 cctgatgaag ggccaccttt acctgatgta ccgttcacat tcccactggc ctgaccacca
    15841 ttgtgtccca gacctgaatt acccggacct cgtccgtctc cgccactcat atacttttcc
    15901 tctttgactt ttaaaacaat aaactaaaat aaaatactgt acatataacc actggtttta
    15961 tatacagtat aaaaattacg ctgccttgtt tttcatgtca agtctgtaaa tttgcgtttt
    16021 tgtgaaaatg atcaaaaaaa cagccactca caccatgcgc aatcccctcc agatgcaaca
    16081 aaaaccagca caaacggcgc cgttctccgg cctcagtatg ttttactgaa atcattatat
    16141 gagataccag aaagccccct ccgggacagg aaaagaaacg ggggtctggt tcgggttgtt
    16201 cggtcactga acacggtcag aaactgatga taagatagcc tgcggatata taactcaatg
    16261 agaaaaaaga attaaaggta aggagacgct catgagacgt tccggcagca aactgtgtct
    16321 tatcgccatg ataacagcga cagcaactct cactgcctgt gctccaaagg gcagcgtgga
    16381 acagcatacc cggcattacg tatatgcttc agatgacggt tttgatccca atttttccac
    16441 ccagaaagct gacacaacac gaatgatggt gccttttttc cggcagttct gggatatggg
    16501 cgcaaaggat aaagcgaccg gaaaatcacg gagtgatgtg cagcaacgca ttcagcagtt
    16561 tcacagtcag gaatttttaa actcactcca aggcacaaca caatttgcgg gtaccgatta
    16621 ccgcagcaaa gacctcacac cgaaaaaatc aaggctactg gctgacacca tttcagctgt
    16681 ttatctcgat ggctatgaag gcagacagta agtggattta ccataacgcg tcattacgcg
    16741 caccgctcag acgggctcag ggcgtttccg gcggctacgg gataaaacag gcaacaaccc
    16801 ccccggaaaa ctgccggaaa cgcgcccgat aaattttatc ctaatgcatg cctatgcacg
    16861 caactgcatg gcatatgttt tgacgtaaaa tggatgccgt aagccctttt ttaccgactt
    16921 tctgcttcat cagcctgact gacacgacca tattctttca gaccgccccc tgctaaactt
    16981 ccttcatatt gccgcattaa cccgggaggc tccgcaatga gcaacactac acactacgaa
    17041 aacgccaact ttcttcggga actggctgaa agcctgcccc gcatactgcc ggaaggtggc
    17101 ccggacaaag ctgcgctgtt gcagcgactg gctaacgaag aactggccca ggcagagtat
    17161 gaagatcagg ttcgcgccaa agtgaccgcc gcccgtgctg acacgcgtcc gggtatgacc
    17221 acggaacaac tccgccagcg cctgcatggc cgttatcagg aactgcgcga tgccgtatga
    17281 cgtggaatgg aagcagggag ccatcgagga tgtaacgacg ctgtttgact atatcgcgga
    17341 aaattcatcc ctgtgggatg cgcagaacgt taccgaccgg gttctcgctg cggcggacaa
    17401 actggccgac ttcccgcgac tctatgaagt ggactcacgc tatggcgagg acgttcgccg
    17461 gatcagtctg atgggacagc atgtcctcta tgatgttgat gaccagacca gaaaagtccg
    17521 ggttctggcc gtggtcggcc agcgccagaa tccccatatt gttctctaat aaccggtgac
    17581 ggggacgcaa ttcattgcgt ggccgtcgcc ggttggacaa gcacagcgcg tcagcgtttt
    17641 ccccacggcc accaccggcg cggtgttccg gccggggtct ctgctgtctc cggtttgtgt
    17701 tccagcagtc tcatggactg catttcctgc cgtagcccgg ccacctcctc cctgagtgct
    17761 tttatctccg cgatcaacgc gtcggaggcc attttttcag tctcacccga cagtctcaac
    17821 tcgtcccgct cgtgagtctc atcctgtctt aactcaccgt acacccggat aagctcgctg
    17881 gtatccacca cccggccgcc atcagcttcc gtacggtatg atacgcggcc tttcgccata
    17941 tcccgataca gggaacttcg ggctttaccc gtcagttccg tcgcctgtct caccgtcacc
    18001 cacgccattg tctcaccccg ctatttttga gacagtatac ctgacaggcg cacaccatgc
    18061 ccggaaggaa agaatcatga caaaacagga aaaaacagcc ctcaatatgg ctaaatttat
    18121 taaggagcag tcgctgctat tgctcgataa actgaacgag ctggatatgg ataccccggc
    18181 cgacatgtgc gagcggctac acgaggatgc ggaaacactc tggcaggtga tgcgggataa
    18241 gctcggggag gaataacagc accggcatgg ccggtgctgg cgtttatttt ttgccgaacg
    18301 gccaccaccg gcgggcaggt ttgtcctccg gcagttcagg taccggcacg ttaaaatagc
    18361 ggctgttata ttcccgcatc agctccgctt tcagcttctc gttcaccgcg ctttccccgt
    18421 gccgctcact caccacgtca atcatcgcgt taatctgctc cggtgagcac ttcccggcat
    18481 caaccacctg cacaccgttc tcgatggcga accgctccac cttgcgatag aggtaatcac
    18541 acagcttcat tcctgtgggt ttgttccgtt cccattgcgg attttta
//
