LOCUS       NZ_CP141559            10334 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP52_L5_46.22_ST3096_NDM5_OXA48 plasmid
            unnamed1, complete sequence.
ACCESSION   NZ_CP141559
VERSION     NZ_CP141559.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38771713
            Assembly: GCF_035596915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Assembly Name                     :: q20run4_BC09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 125x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 12:06:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,543
            CDSs (total)                      :: 5,420
            Genes (coding)                    :: 5,320
            CDSs (with protein)               :: 5,320
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 51 of 100
            Pseudo Genes (incomplete)         :: 54 of 100
            Pseudo Genes (internal stop)      :: 15 of 100
            Pseudo Genes (multiple problems)  :: 16 of 100
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141559.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP52_L5_46.22_ST3096_NDM5_OXA48"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed1"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-11-10"
                     /genotype="ST3096"
     gene            complement(join(10155..10334,1..354))
                     /locus_tag="VI613_RS00005"
                     /old_locus_tag="VI613_00005"
     CDS             complement(join(10155..10334,1..354))
                     /locus_tag="VI613_RS00005"
                     /old_locus_tag="VI613_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(357..1283)
                     /locus_tag="VI613_RS00010"
                     /old_locus_tag="VI613_00010"
     CDS             complement(357..1283)
                     /locus_tag="VI613_RS00010"
                     /old_locus_tag="VI613_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1460..1858
                     /locus_tag="VI613_RS00015"
                     /old_locus_tag="VI613_00015"
     CDS             1460..1858
                     /locus_tag="VI613_RS00015"
                     /old_locus_tag="VI613_00015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(2062..2976)
                     /locus_tag="VI613_RS00020"
                     /old_locus_tag="VI613_00020"
     CDS             complement(2062..2976)
                     /locus_tag="VI613_RS00020"
                     /old_locus_tag="VI613_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            4713..4922
                     /locus_tag="VI613_RS00025"
                     /old_locus_tag="VI613_00025"
     CDS             4713..4922
                     /locus_tag="VI613_RS00025"
                     /old_locus_tag="VI613_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(4988..5521)
                     /locus_tag="VI613_RS00030"
                     /old_locus_tag="VI613_00030"
     CDS             complement(4988..5521)
                     /locus_tag="VI613_RS00030"
                     /old_locus_tag="VI613_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(5524..6450)
                     /locus_tag="VI613_RS00035"
                     /old_locus_tag="VI613_00035"
     CDS             complement(5524..6450)
                     /locus_tag="VI613_RS00035"
                     /old_locus_tag="VI613_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            6627..7025
                     /locus_tag="VI613_RS00040"
                     /old_locus_tag="VI613_00040"
     CDS             6627..7025
                     /locus_tag="VI613_RS00040"
                     /old_locus_tag="VI613_00040"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(7229..8143)
                     /locus_tag="VI613_RS00045"
                     /old_locus_tag="VI613_00045"
     CDS             complement(7229..8143)
                     /locus_tag="VI613_RS00045"
                     /old_locus_tag="VI613_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            9880..10089
                     /locus_tag="VI613_RS00050"
                     /old_locus_tag="VI613_00050"
     CDS             9880..10089
                     /locus_tag="VI613_RS00050"
                     /old_locus_tag="VI613_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          complement(2202..2976)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(7369..8143)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(361..1283)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5528..6450)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctataccct ttaagggtgt tgtattcctg cacgatattg ctgtgaattg tcgcaatatc
       61 cctgttcagg gcatccgcgt ttgccttcat gtttgcgact gactcttcca gccgctgaaa
      121 atcctggctg atgcgctcac ctatcagtcc tgccacgtcc agcttctgca cctcatttct
      181 gacttcatcc ctgacctgtt ttgccacacc gtcagaaata tcccgatatc cgcgaagagt
      241 ttcccttgcc acagccagag aatcagcaac agctttctgt tgctccattg cggtatgttg
      301 cagatgctcc accgataaca ggagcgtaag taatttttct tctgtgctgg ccatgttcag
      361 aatcccatag aaaatccacg tgacggtgat ttaacccgtt caggtgcagt tcggttctgt
      421 ctgctcactc tgtctctcat gtcaaagcca tgctcccgcg caacatcatg ttgcagcctt
      481 ctgaccagtt catgttgctt aaccggagtg tccagccatt ccctgatgtc atgcccgacc
      541 gttcgcgcct gttcaagatg gcgggaatag tccggttcag accgtttttg catgatgtca
      601 tgacgctggg catcatcaag ccgtccggca gcttcaaaac ccagatgcac ttcgggacgt
      661 cgttcaatgc cctgttcttt cagcgtccgg cagtcaatga agcccctttc aaagccatgc
      721 tttctcatat gttcgttgtg cttcacctcc cagcgttttc gcaattcccg caactgctct
      781 ttttgctgag tgagagtcag gcttccggaa tcctttttcg cacctccccg ctcgggatac
      841 ctggcgttat agcgccggaa atactgttcc ggtgtacgtt caatgccatc attcacacgc
      901 tgactcatca tgatatgcgc atgaggctgt tcgccgccgt caatgctcgc tttcggattg
      961 tgaatggcaa aactccaggc gtgccgttcg ccagcttcct gacgtacaaa atcctgcacc
     1021 agttcaaggc gctgttccgg tgtcagttca cggggtaatg caatttcaag ttccctatag
     1081 gtcgaaccgt tcgcacgttc aaactggtca gccgcctgcc agaaatggga aggattatcc
     1141 ctggcccatt ccggcatgtt tccgtgtgcg gaatattcca aatcctgacg gtcgcggtat
     1201 ttctctttgc gctcgatgta atcggcgtgg tttgcagcct gccccttgcc accaaattta
     1261 acgctgaggt gataggttgc cacggtttca gccctctgca ggagcgcact gtttctcgac
     1321 gaaggagaga aacatataag tgcgcttcct tctaatttta cgaattagca cggaagatac
     1381 cccctgcacc cgccgaccgc aagcgatata ctgcacgacc acaacacacc gcaaaaaaac
     1441 accagggagc atcacgaaca tgcgcatcag caacatcgaa tggctgaaga aacggatcgg
     1501 attcatcagg aagctcggag aacagaccgc acgacagaga cagattattg acctgatcga
     1561 caacgaagcc ggactcacag aacaggaacg caaacttctc cacgtactgg caacagcgga
     1621 aaaaaacgac ttacaggcac aggagagcga acgaaaacag gcggtccaaa aacgaatcga
     1681 gggaaaaaaa caacgacggg aacgcaatca ccggctcttt ctcgcggctg ggctactgat
     1741 cgaagcagga ctggtagaca caaaaacagg cgagctatgc tataaaaaag acaggatatt
     1801 acaagcactt aaggaactta agtacgatct ggagacatcc ccaaacccag acgcgtaagt
     1861 gcctccatcc gtgttccgat tccctggtgc ttcctcgctc attcgctcgc tacgctcggt
     1921 catgagactg cggcgagcac taccaccaca atcacgcgga aagccgcgcc attgcagggc
     1981 tgaacgggga aaaacggcaa aaaacctgct gctcaaaaag acgccagaaa ggcgctgtta
     2041 tctgatatag gcttatttgg tctactctac gagacctcgt tttttcagtt ggtaatacca
     2101 ggcgcgactg atccctaatg cctcccacgg tttcagcgtg cgtgcagatg tagctactgt
     2161 gctgcgctta gacttagaac cgcccttgcg accgcgtgca gcctgaattt ctggcgtgtg
     2221 cgtatcagcc acatactgtg cgaaagtttc cggcgtgaag ttcctgtgcg tgtatttcgc
     2281 aatgctcttg ccaatagccc gacattcagc cagtgaaagc ggaacgggaa gcgatgcatt
     2341 gtacatttcg acacgctgga tcaccgcttc aagccactgt gagaacgctg gccagccctg
     2401 acgaatcgcc ctgtaagccc atttgcgcgt catttcgaac aggtggcagt ttcgccccat
     2461 cccgtaatgt ttatcgatgc tacgacgctc tgaggcgctc aaatcgagat aatcagccag
     2521 ttcatcgagg gtataggctt cctcgcgcca ctccatcacc agccattcca ggtggaacgg
     2581 atttttgcaa atcaggccgc tgtagttcac atccgcacaa agtttctcac acagcgaacg
     2641 ttcaaccgct gcggcatatt tcagcgcctt aaccgaggca tcaggcgcgg ttctcacggc
     2701 gatgttgagt gcgtagagca ggtgagcgtg tccgttcaca ggatttttaa cggtgatgtt
     2761 cggcgcggga gcgttccggt cgctccagtc aatcgcagcc ccctcgcggt caacgtcaaa
     2821 aaccagccag aactgcgcat gaggctggtt ctgctgtatg taccgcgcaa gaagcgcacg
     2881 ccctttgccg gagatgcgaa gaccaaaagc gagatcatcc gtgtgatagg gacgatgggg
     2941 taaattttcg tcgaagtatt gaagcgcggc gctcatgtgt tacgattctc tccgggttat
     3001 gcgacctaca cgccctctgt atttcatgtt gaatcttgcc ggatctggac atgatttcag
     3061 gggcgtttgt ttttcagtcg cttacactac acccaacggc aaatcctgtc gaaccttccc
     3121 gaaaacccga tccatttacc ataactcccg ttgtacgccc cccttaaaac gccctcagtc
     3181 gctttctggc gcaaaaaacg caaaaagaca agaaaacacc aggccaaacc agaaaaacgc
     3241 gtcagaatcg ttttcagggc gttgcataac tatgcacgaa agcgatgtgc gatcggtttc
     3301 gtgcgttctg aacaccgaac ggctcaaaac gacaagtttt ctcccctggt gcgtggcgct
     3361 ctgacggggt ttcctgtttc caaacttcac atctcgccag gacaaaaaaa aaccgacctg
     3421 gggtcggtgg caagaatcag agtctatggg aaatatttta gttatcggat gctcaatatt
     3481 cacaaacgca aaaaatgcct cagtgatgcc ctaaaacaac ctttccagaa ggagtccgcg
     3541 ctttacgatg cacggaagag ataaaaaacc ccacaaaagt ggggaaccaa gagaaacaac
     3601 aattacaggt tgttggttgt gttcgaacaa cgatgaagta gctacgctaa ataataatta
     3661 ccgtacaggc cctacatatt caacactaaa tttaagatcc tgacgaagga tttataaatg
     3721 cacgaaaaca catagacaga caaattcact aaattcatac acaaaaacaa gtgtagtttt
     3781 ctaagtaaat tagattcgac aaccaattag tttatccaca tcgcattcca ctcttgctac
     3841 accaatcaca tttcttgata tgtaatagca gaaaaaataa aaccagacac caatagacca
     3901 aaaaacaaac attcaacaag aaacattttg aacaatatca caaaactgtc acaaccatca
     3961 tcagactatt gaaaaaaact ggttgctgtt ttataaaagc gactgtaaca acctccactc
     4021 tatagttcca ttagtttaaa gtgtcacaca aactgagcat ccatttgttt tccttacttt
     4081 tgcccctgta ttccaagggg tttttttata acttaaagat tagcacacca cacaaaaaca
     4141 ataaaaaaaa acgacaagtt atacaataaa accagtacta tccatacagc taattacata
     4201 taaatactat taaaccatct ggacattaaa aataaactct tataacataa aattaatgcg
     4261 gttccggtac catatgtcag ggataatgtg caaatggagg tatcggaact gcatttcagt
     4321 tcattattaa ttaatgacaa aaaaaacctc tgcgagcgca gagggggtaa ggaatgttcc
     4381 cggtcataat atgtcgatag tgtttatata catacagaac aagaacaaca accagccaac
     4441 taataaatat tttatttttt agagctgaaa ccttaaagct cacttccatt ccatacattg
     4501 ttataaacct tatgttgaaa actgaaagta agtacggcag acttattaag ttgcgtgcag
     4561 tcttttcacg ttatacaaac aagaacacct gcgaagttat ttctccttac atatccccct
     4621 atgccaacgg gggatttttt ctggttcact aacgcataaa taatagtgac accctatcat
     4681 tcacaaaccg tgataatata aggattatca tcatggcaaa attaggtgaa aacaccccac
     4741 atattatcga tgctactgta gattttatgg catcaagcca ggcatttcgt gagtatctga
     4801 aaaaacttcc ccctgataac acaataccgc cagctcttac tgatgagcac gtaccgttat
     4861 atatacgaag gctggaatac tatcgtctgc tttatcgacc aaagcagaca gaatcatatt
     4921 gaaaaaaacg gtgaacggtt cgcgaaattc tttcgcgggt cgtgaggcgt tggacaagcg
     4981 aagcgcgtca gtttttccgg ctcttccccg tgacaggttt ctccaccagt ttatcttcca
     5041 gtgtcacaac tttctgatac accgccgctg tggcgttgta gttcctgtcg agtattgttt
     5101 tcattcccca tccgagcaca cagaacataa ccagaaaagc cactgacacc ccacccagcc
     5161 atttccagct atacccttta agggtgttgt attcctgcac gatattgctg tgaattgtcg
     5221 caatatccct gttcagggca tccgcgtttg ccttcatgtt tgcgactgac tcttccagcc
     5281 gctgaaaatc ctggctgatg cgctcaccta tcagtcctgc cacgtccagc ttctgcacct
     5341 catttctgac ttcatccctg acctgttttg ccacaccgtc agaaatatcc cgatatccgc
     5401 gaagagtttc ccttgccaca gccagagaat cagcaacagc tttctgttgc tccattgcgg
     5461 tatgttgcag atgctccacc gataacagga gcgtaagtaa tttttcttct gtgctggcca
     5521 tgttcagaat cccatagaaa atccacgtga cggtgattta acccgttcag gtgcagttcg
     5581 gttctgtctg ctcactctgt ctctcatgtc aaagccatgc tcccgcgcaa catcatgttg
     5641 cagccttctg accagttcat gttgcttaac cggagtgtcc agccattccc tgatgtcatg
     5701 cccgaccgtt cgcgcctgtt caagatggcg ggaatagtcc ggttcagacc gtttttgcat
     5761 gatgtcatga cgctgggcat catcaagccg tccggcagct tcaaaaccca gatgcacttc
     5821 gggacgtcgt tcaatgccct gttctttcag cgtccggcag tcaatgaagc ccctttcaaa
     5881 gccatgcttt ctcatatgtt cgttgtgctt cacctcccag cgttttcgca attcccgcaa
     5941 ctgctctttt tgctgagtga gagtcaggct tccggaatcc tttttcgcac ctccccgctc
     6001 gggatacctg gcgttatagc gccggaaata ctgttccggt gtacgttcaa tgccatcatt
     6061 cacacgctga ctcatcatga tatgcgcatg aggctgttcg ccgccgtcaa tgctcgcttt
     6121 cggattgtga atggcaaaac tccaggcgtg ccgttcgcca gcttcctgac gtacaaaatc
     6181 ctgcaccagt tcaaggcgct gttccggtgt cagttcacgg ggtaatgcaa tttcaagttc
     6241 cctataggtc gaaccgttcg cacgttcaaa ctggtcagcc gcctgccaga aatgggaagg
     6301 attatccctg gcccattccg gcatgtttcc gtgtgcggaa tattccaaat cctgacggtc
     6361 gcggtatttc tctttgcgct cgatgtaatc ggcgtggttt gcagcctgcc ccttgccacc
     6421 aaatttaacg ctgaggtgat aggttgccac ggtttcagcc ctctgcagga gcgcactgtt
     6481 tctcgacgaa ggagagaaac atataagtgc gcttccttct aattttacga attagcacgg
     6541 aagatacccc ctgcacccgc cgaccgcaag cgatatactg cacgaccaca acacaccgca
     6601 aaaaaacacc agggagcatc acgaacatgc gcatcagcaa catcgaatgg ctgaagaaac
     6661 ggatcggatt catcaggaag ctcggagaac agaccgcacg acagagacag attattgacc
     6721 tgatcgacaa cgaagccgga ctcacagaac aggaacgcaa acttctccac gtactggcaa
     6781 cagcggaaaa aaacgactta caggcacagg agagcgaacg aaaacaggcg gtccaaaaac
     6841 gaatcgaggg aaaaaaacaa cgacgggaac gcaatcaccg gctctttctc gcggctgggc
     6901 tactgatcga agcaggactg gtagacacaa aaacaggcga gctatgctat aaaaaagaca
     6961 ggatattaca agcacttaag gaacttaagt acgatctgga gacatcccca aacccagacg
     7021 cgtaagtgcc tccatccgtg ttccgattcc ctggtgcttc ctcgctcatt cgctcgctac
     7081 gctcggtcat gagactgcgg cgagcactac caccacaatc acgcggaaag ccgcgccatt
     7141 gcagggctga acggggaaaa acggcaaaaa acctgctgct caaaaagacg ccagaaaggc
     7201 gctgttatct gatataggct tatttggtct actctacgag acctcgtttt ttcagttggt
     7261 aataccaggc gcgactgatc cctaatgcct cccacggttt cagcgtgcgt gcagatgtag
     7321 ctactgtgct gcgcttagac ttagaaccgc ccttgcgacc gcgtgcagcc tgaatttctg
     7381 gcgtgtgcgt atcagccaca tactgtgcga aagtttccgg cgtgaagttc ctgtgcgtgt
     7441 atttcgcaat gctcttgcca atagcccgac attcagccag tgaaagcgga acgggaagcg
     7501 atgcattgta catttcgaca cgctggatca ccgcttcaag ccactgtgag aacgctggcc
     7561 agccctgacg aatcgccctg taagcccatt tgcgcgtcat ttcgaacagg tggcagtttc
     7621 gccccatccc gtaatgttta tcgatgctac gacgctctga ggcgctcaaa tcgagataat
     7681 cagccagttc atcgagggta taggcttcct cgcgccactc catcaccagc cattccaggt
     7741 ggaacggatt tttgcaaatc aggccgctgt agttcacatc cgcacaaagt ttctcacaca
     7801 gcgaacgttc aaccgctgcg gcatatttca gcgccttaac cgaggcatca ggcgcggttc
     7861 tcacggcgat gttgagtgcg tagagcaggt gagcgtgtcc gttcacagga tttttaacgg
     7921 tgatgttcgg cgcgggagcg ttccggtcgc tccagtcaat cgcagccccc tcgcggtcaa
     7981 cgtcaaaaac cagccagaac tgcgcatgag gctggttctg ctgtatgtac cgcgcaagaa
     8041 gcgcacgccc tttgccggag atgcgaagac caaaagcgag atcatccgtg tgatagggac
     8101 gatggggtaa attttcgtcg aagtattgaa gcgcggcgct catgtgttac gattctctcc
     8161 gggttatgcg acctacacgc cctctgtatt tcatgttgaa tcttgccgga tctggacatg
     8221 atttcagggg cgtttgtttt tcagtcgctt acactacacc caacggcaaa tcctgtcgaa
     8281 ccttcccgaa aacccgatcc atttaccata actcccgttg tacgcccccc ttaaaacgcc
     8341 ctcagtcgct ttctggcgca aaaaacgcaa aaagacaaga aaacaccagg ccaaaccaga
     8401 aaaacgcgtc agaatcgttt tcagggcgtt gcataactat gcacgaaagc gatgtgcgat
     8461 cggtttcgtg cgttctgaac accgaacggc tcaaaacgac aagttttctc ccctggtgcg
     8521 tggcgctctg acggggtttc ctgtttccaa acttcacatc tcgccaggac aaaaaaaaac
     8581 cgacctgggg tcggtggcaa gaatcagagt ctatgggaaa tattttagtt atcggatgct
     8641 caatattcac aaacgcaaaa aatgcctcag tgatgcccta aaacaacctt tccagaagga
     8701 gtccgcgctt tacgatgcac ggaagagata aaaaacccca caaaagtggg gaaccaagag
     8761 aaacaacaat tacaggttgt tggttgtgtt cgaacaacga tgaagtagct acgctaaata
     8821 ataattaccg tacaggccct acatattcaa cactaaattt aagatcctga cgaaggattt
     8881 ataaatgcac gaaaacacat agacagacaa attcactaaa ttcatacaca aaaacaagtg
     8941 tagttttcta agtaaattag attcgacaac caattagttt atccacatcg cattccactc
     9001 ttgctacacc aatcacattt cttgatatgt aatagcagaa aaaataaaac cagacaccaa
     9061 tagaccaaaa aacaaacatt caacaagaaa cattttgaac aatatcacaa aactgtcaca
     9121 accatcatca gactattgaa aaaaactggt tgctgtttta taaaagcgac tgtaacaacc
     9181 tccactctat agttccatta gtttaaagtg tcacacaaac tgagcatcca tttgttttcc
     9241 ttacttttgc ccctgtattc caaggggttt ttttataact taaagattag cacaccacac
     9301 aaaaacaata aaaaaaaacg acaagttata caataaaacc agtactatcc atacagctaa
     9361 ttacatataa atactattaa accatctgga cattaaaaat aaactcttat aacataaaat
     9421 taatgcggtt ccggtaccat atgtcaggga taatgtgcaa atggaggtat cggaactgca
     9481 tttcagttca ttattaatta atgacaaaaa aaacctctgc gagcgcagag ggggtaagga
     9541 atgttcccgg tcataatatg tcgatagtgt ttatatacat acagaacaag aacaacaacc
     9601 agccaactaa taaatatttt attttttaga gctgaaacct taaagctcac ttccattcca
     9661 tacattgtta taaaccttat gttgaaaact gaaagtaagt acggcagact tattaagttg
     9721 cgtgcagtct tttcacgtta tacaaacaag aacacctgcg aagttatttc tccttacata
     9781 tccccctatg ccaacggggg attttttctg gttcactaac gcataaataa tagtgacacc
     9841 ctatcattca caaaccgtga taatataagg attatcatca tggcaaaatt aggtgaaaac
     9901 accccacata ttatcgatgc tactgtagat tttatggcat caagccaggc atttcgtgag
     9961 tatctgaaaa aacttccccc tgataacaca ataccgccag ctcttactga tgagcacgta
    10021 ccgttatata tacgaaggct ggaatactat cgtctgcttt atcgaccaaa gcagacagaa
    10081 tcatattgaa aaaaacggtg aacggttcgc gaaattcttt cgcgggtcgt gaggcgttgg
    10141 acaagcgaag cgcgtcagtt tttccggctc ttccccgtga caggtttctc caccagttta
    10201 tcttccagtg tcacaacttt ctgatacacc gccgctgtgg cgttgtagtt cctgtcgagt
    10261 attgttttca ttccccatcc gagcacacag aacataacca gaaaagccac tgacacccca
    10321 cccagccatt tcca
//
