LOCUS       NZ_CP141553            10415 bp    DNA     circular CON 17-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP67_L13_49.22_ST437_NDM5_OXA232
            plasmid unnamed, complete sequence.
ACCESSION   NZ_CP141553
VERSION     NZ_CP141553.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38771715
            Assembly: GCF_035594725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10415)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Plasmid analysis using q20 nanopore sequencing
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10415)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Assembly Name                     :: rrp8_bc05
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 38x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/12/2024 12:04:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,362
            CDSs (total)                      :: 5,238
            Genes (coding)                    :: 5,063
            CDSs (with protein)               :: 5,063
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 175
            CDSs (without protein)            :: 175
            Pseudo Genes (ambiguous residues) :: 0 of 175
            Pseudo Genes (frameshifted)       :: 117 of 175
            Pseudo Genes (incomplete)         :: 66 of 175
            Pseudo Genes (internal stop)      :: 16 of 175
            Pseudo Genes (multiple problems)  :: 23 of 175
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141553.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10415
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP67_L13_49.22_ST437_NDM5_OXA232"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-12-02"
                     /genotype="ST437"
     gene            complement(<271..366)
                     /locus_tag="VI617_RS00680"
                     /old_locus_tag="VI617_00680"
                     /pseudo=""
     CDS             complement(<271..366)
                     /locus_tag="VI617_RS00680"
                     /old_locus_tag="VI617_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /name="MbeB family mobilization protein"
     gene            complement(<407..835)
                     /locus_tag="VI617_RS00685"
                     /old_locus_tag="VI617_00685"
                     /pseudo=""
     CDS             complement(<407..835)
                     /locus_tag="VI617_RS00685"
                     /old_locus_tag="VI617_00685"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1053..1394)
                     /locus_tag="VI617_RS00690"
                     /old_locus_tag="VI617_00690"
     CDS             complement(1053..1394)
                     /locus_tag="VI617_RS00690"
                     /old_locus_tag="VI617_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_324669560.1"
                     /translation="MADKRNKMLTMWVTEDEHRQLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNSLNRIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2010..2123
                     /locus_tag="VI617_RS00695"
                     /old_locus_tag="VI617_00695"
     CDS             2010..2123
                     /locus_tag="VI617_RS00695"
                     /old_locus_tag="VI617_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(2343..3152)
                     /locus_tag="VI617_RS00700"
                     /old_locus_tag="VI617_00700"
     CDS             complement(2343..3152)
                     /locus_tag="VI617_RS00700"
                     /old_locus_tag="VI617_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(3145..4395)
                     /locus_tag="VI617_RS00705"
                     /old_locus_tag="VI617_00705"
     CDS             complement(3145..4395)
                     /locus_tag="VI617_RS00705"
                     /old_locus_tag="VI617_00705"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="WP_001584442.1"
                     /translation="MTRPIAVDLFAGAGGLSLGFEQAGFDIMASVEIDPIHCSAHKYNF
                     PMCKTICRSVVDVTGEYVREVSGIGEKEIDVVIGGAPCQGFSLIGKRALDDDRNKLVYH
                     YVRLVLELKPKYFVFENVKGLTVGKHKQFLVEIMDAFKDGGYDVVSDYKVLNAADYGVP
                     QDRRRLFLMGGRKGIALPSYPEPLSIRTTVGDAIGDIPDAEKYPELWGRDWVYAKFGEQ
                     SLYSSYLHGVGKDPSDFSYPRIFNKDKLTCSLLTDHSDISRGRFVETLPDTVEPISRFK
                     KLSMNGLCNTLRAGTASDRGAFTSPRPIHPIYPRVITVREAARLHSYPDWFRFHVTKWH
                     GFRQVGNSVPPMLARSVGLQIMKVLGVTPKKPTKKIKLGDESLVAISMSDAASMFGVSS
                     NVIAKRIKNSNKEVVCE"
                     /name="DNA cytosine methyltransferase"
     gene            complement(4847..5062)
                     /locus_tag="VI617_RS00710"
                     /old_locus_tag="VI617_00710"
     CDS             complement(4847..5062)
                     /locus_tag="VI617_RS00710"
                     /old_locus_tag="VI617_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(5069..5566)
                     /locus_tag="VI617_RS00715"
                     /old_locus_tag="VI617_00715"
     CDS             complement(5069..5566)
                     /locus_tag="VI617_RS00715"
                     /old_locus_tag="VI617_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_015328238.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLHMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5607..6305)
                     /locus_tag="VI617_RS00720"
                     /old_locus_tag="VI617_00720"
                     /pseudo=""
     CDS             complement(<5607..6305)
                     /locus_tag="VI617_RS00720"
                     /old_locus_tag="VI617_00720"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6244..6585)
                     /locus_tag="VI617_RS00725"
                     /old_locus_tag="VI617_00725"
     CDS             complement(6244..6585)
                     /locus_tag="VI617_RS00725"
                     /old_locus_tag="VI617_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7208..7339
                     /locus_tag="VI617_RS00730"
                     /old_locus_tag="VI617_00730"
     CDS             7208..7339
                     /locus_tag="VI617_RS00730"
                     /old_locus_tag="VI617_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072007956.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPKRHAKAPLAAATGNRIHGAE"
                     /name="replication initiation protein"
     gene            complement(7559..8368)
                     /locus_tag="VI617_RS00735"
                     /old_locus_tag="VI617_00735"
     CDS             complement(7559..8368)
                     /locus_tag="VI617_RS00735"
                     /old_locus_tag="VI617_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010222407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032446875.1"
                     /translation="MSKDKKERSKNRYHQLVEKIFFDKYVDGDLEVLFERLDINSAAKD
                     LGIELPSNVGDVIYSIRFRTPMPESILLTQPEGKEWIIELAGRSKYRFRLVTISRILPN
                     PNMVAIKIPDNTPEIITGYSLDDEQALLAKVRYNRLIDIFLGITTYSLQNHLRTTVKGI
                     GQIEIDELYVGIDKYGCHYIIPVQAKGGTDQISMVQTSQDTAWCEQKYPNVRCRAISAQ
                     FVSSDLIALFELKIDEGQVKLIEERHYKLVPVKELDSSDISNYRMSV"
                     /name="hypothetical protein"
     gene            complement(8361..9612)
                     /locus_tag="VI617_RS00740"
                     /old_locus_tag="VI617_00740"
                     /pseudo=""
     CDS             complement(8361..9612)
                     /locus_tag="VI617_RS00740"
                     /old_locus_tag="VI617_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015160339.1"
                     /GO_function="GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO:0008168 - methyltransferase activity [Evidence IEA];
                     GO:1904047 - S-adenosyl-L-methionine binding [Evidence
                     IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /name="DNA cytosine methyltransferase"
     gene            complement(10060..10277)
                     /locus_tag="VI617_RS00745"
                     /old_locus_tag="VI617_00745"
                     /pseudo=""
     CDS             complement(10060..10277)
                     /locus_tag="VI617_RS00745"
                     /old_locus_tag="VI617_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          complement(1479..1654)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="88.268"
                     /coverage="81.0"
                     /evalue="2.69e-52"
     Typing          complement(6670..6845)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.449"
                     /coverage="81.0"
                     /evalue="5.74e-59"
     Typing          1713..2123
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.478"
                     /coverage="99.0"
                     /evalue="1.65e-171"
     Typing          6792..7317
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.434"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(3..1063)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.678"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1060..1373)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.623"
                     /coverage="99.0"
                     /evalue="8.250000000000003e-57"
     Typing          complement(4732..6254)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.803"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6251..6564)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.0500000000000001e-58"
ORIGIN
        1 catgtgcagt ttcgacagcg tatctctctg ctgcctgatt tccgccaggt tcgccgctat
       61 ccggttcccc tggtaccaca gcaccccgct caggctggcg aacagcagcc ctgacaccat
      121 ggtgatggtc agccacgtcc gcccgaccat tcgcagcacg ctcagccggt tcgactgcat
      181 cgccgcgttc atcccctgtt cgtgggcgct gatggcgtcg ttgattctct tcgcgcttgc
      241 gttcagttcc gatttgacaa cgttctcgtg cttgctgaat gcggctttca gcctctcttc
      301 ggtaagctgc tgctgtgctt tcgatttctc ctcgaagtcc ctcgaggccg ttaaaagact
      361 gctcataaat ggctcccctc agtcggatat ttcgcccccc gtccgggtcg gcaatgctga
      421 tactgttttt tgtggttctg accacctcaa aaccggccgc tgtgagcgcc cctgtgacgt
      481 catcacgcgt tttcagctct cctgatgagg ctaacatcag caaaccccgt gtaatcgtct
      541 ctgcggcttc ctgcttcgtt tctggcagtc ctgaaggcgt gaccagcgcc cgccggttct
      601 ccgggtcgtt cggatcatgt agccccagcc tgccgttgac cactgtctgc cacgcatcga
      661 tgcgcggccg gtcggcacga tcgtaatacg gctggaggcg tttccccgtc agtaattcgg
      721 tattggggat cagaaaattc agctcaagac gacccttgtc tgcgtgttcc acccacagaa
      781 tgctgtactg gtctttatcg agtccgggca tcagaacccg ctcaaagctg gccatcagtt
      841 tttcacgctg acccggcggc aggtccgcct ccgcgaatga cagcaccccg gaggtatatt
      901 ttttcacata cggcgacgaa tcctcggatc agctcccgga cttcttccgg cttcccctgc
      961 aggacgctgg caccttcacg ctgccggtct ttacccagac atacttgata atcaaagtac
     1021 aggcgtctcg cccgcgaccc cggggatgga atttagcaat catcgtccgg ccctttttcc
     1081 agtacggcat gccgcaaccg ttccagaccg gcatcgatcg ccatcagcgc tgccacgatc
     1141 tgcacccggt catgaccggc accaccggcg ttcactttcc gggcgattcg gttcaggcta
     1201 ttgcccatgc cggcaagctg acgcagcagc gcgggcgaaa tcgagggaag cctgccggcg
     1261 cgtgtcggtt tctcatcaag gcaggtctgg cgcatccacg ccgccagttg ttttccgtcg
     1321 caccgctcaa gcaactgccg gtgttcgtct tccgtcaccc acatcgtgag catcttgttg
     1381 cgtttatctg ccagaatcgc ctcctgataa cgccgtgacg gacgggaaag cctttcccgg
     1441 tcggcgcggg gttggacaag ccgcaggcgc gtcagtgggg tttcggggcg cagccctgaa
     1501 ccagtcacgt agcgctagcg gagtgaatac tggcttagta tgttacggcg agacggatat
     1561 tcggggaagt gcttcatgtg gcaaggggaa aaatacggct gcggggcgta agtgcaaaat
     1621 gcaggtaacg atgaaatcac gcttcctcgc tcactcagtc gctacgctcc tgccgttcgg
     1681 ctgcggcgag cggcaggagc tcgcgataaa agctcgagaa aaccgagcga tcgcgctgaa
     1741 gtgggagaga ggagaggccg cacagcggcc gtttttccat aggctccgcc cccctgacaa
     1801 gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata
     1861 ccaggcgttt ccccctggtg gctccctcgt gcgctctcct gttcctgcct ttcggtttac
     1921 cggtgtcatt ccgctgttat ggccgcgttt atctcattcc acgcctgaca ctcagttccg
     1981 ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgactgct
     2041 gcgccttttc cggtaactat caacttgagt ccaacccgga aagacacgac gaagcgccac
     2101 tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg tgccagttaa
     2161 ggctaaactg aaagaacgat tttggttact gcgctcctct aactagccag ttaccttcgg
     2221 aagaaagagt tggtagctct aaattccggc aaacgaacca ccgttggtag cggtggtttt
     2281 tttgtttacg gcttaggaga ttacggctag aacgaaagga tctcaagaaa tcctctcaga
     2341 aattatacag acatgcggta gtttgagatg tcagagctat ctaactcttt taccggaacc
     2401 agtttgtaat gcctttcctc aatcaatttc acttgtcctt cgtctatctt tagctcaaac
     2461 agtgcaatca aatcgctcga aacaaactga gcggaaatag ctctacatct aacatttggg
     2521 tatttttgtt cacaccacgc tgtatcttgc gatgtttgca ccattgatat ttgatctgtc
     2581 ccaccttttg cttgaactgg tattatataa tggcaaccgt atttatctat acctacataa
     2641 agttcatcga tctctatttg accaattccc ttcaccgtag ttcttaaatg attctgtaaa
     2701 gaatatgtgg ttatacctag aaatatatct attagccggt tatatctcac cttcgccagc
     2761 aatgcctgct cgtcgtctaa agaataacct gtaattatct ccggggtatt gtctggaatt
     2821 tttattgcca ccatattagg atttggcaaa atcctactaa tagttactaa tctaaatcga
     2881 tactttgacc taccagccag ctcaataatc cactccttac cttctggctg agtcaacaga
     2941 atactttctg gcataggcgt tctaaatctg attgaatata taacatcacc aacattagat
     3001 gggagttcta tacctaaatc ttttgcagca ctgtttatat ctaacctttc aaacaaaacc
     3061 tctaagtcac catcaacata tttatcgaaa aatatctttt ctaccaactg atggtatcta
     3121 tttttggacc tttctttttt atccttactc acaaacaacc tccttgttac tattttttat
     3181 tctcttagca attacatttg atgacacacc aaacatagat gcagcatcgg acatagaaat
     3241 agcaaccaaa ctttcatcac cgagtttgat tttcttcgtt ggtttttttg gcgtaacacc
     3301 aagaaccttc ataatctgca agccaacact tctagctagc ataggaggaa cactattacc
     3361 gacctgtcta aatccatgcc acttagtcac atgaaatctg aaccaatcag gatatgaatg
     3421 cagtcttgct gcctctctga ccgtgatgac cctaggatag atcggatgta taggacgagg
     3481 agatgtaaaa gcacctctgt cacttgctgt tcctgccctt aaagtattac atagcccatt
     3541 catcgagagc tttttaaacc tgctgattgg ttctaccgtg tcaggcaaag tttcaacaaa
     3601 ccgccctcta gaaatgtcag agtgatctgt cagtaaagaa cacgttaatt tgtctttatt
     3661 aaaaatcctt gggtagctaa aatcactagg atcttttcct acgccatgca aataggatga
     3721 gtatagtgat tgctcaccaa atttagcata aacccaatct ctaccccata gctcaggata
     3781 tttttccgca tcaggaatat ccccaatagc gtccccaacc gttgttctaa tggataatgg
     3841 ctcagggtat gatggcagag caattccttt ccttcccccc attaagaaaa gacgacggcg
     3901 atcttgagga actccataat cagctgcgtt tagcacttta taatcgctta caacgtcgta
     3961 cccgccatct ttaaatgcat ccattatctc tactaaaaac tgcttatgct taccaaccgt
     4021 caaccctttt acattttcaa aaacaaagta tttaggcttt agctcaagaa ccagccgtac
     4081 atagtgatag accagtttgt ttctatcatc atcaagcgcg cgttttccta ttaatgaaaa
     4141 gccttggcag ggagcgcctc cgatcactac atcaatctct ttctcaccaa tacctgaaac
     4201 ttccctaacg tattctccgg taacatcaac aacacttcta catatcgttt tgcacatagg
     4261 aaagttatat ttgtgagcag agcaatgtat tggatctatt tcaactgatg ccattatatc
     4321 aaagccagct tgctcgaaac cgagactcag cccacccgct ccagcaaata aatcaacagc
     4381 aatcggtcta gtcattcttt acacccacgt tatataaaat caacatgatg ctatcagaaa
     4441 aacgatcgct ttaccgatcg tttttctgat ttcgattgaa cataacatct cttacccgca
     4501 ccgcgccgga ggcactcagg gcgtttctga tggctgacgg gcaaaaggtg gtggcaagta
     4561 caccgaaaac caccagaaac gcacctgatt tattttacgc atatgcatca cttattcaca
     4621 tcactgcata gccggtgatt tcggttaaaa aagactctct gtgtgctgaa atcgcagaaa
     4681 atggccatga tccccgtcac gctcaggcca cctgagacgg gcgtttatca gtctccatac
     4741 tccctgtcac catgagataa tgtgcgttgc cgttccggta ccagatctgc tgtcttctct
     4801 ggttcctttt ccgttctttg cccgtatcgc tgttgctcaa tctcattcag cctgtcgata
     4861 accggcgcag ctgctcgctc aaactctgca cctgcctgct caagcccgac acgcgctcgc
     4921 tcagcgccgc gttctcccgt tgcataagac cagacatctt ccgccattcc tcgaaggcgc
     4981 tctcccactc gtccagcctt ctcgagtagt cctgctgtag ctgctctaac gcgctcagca
     5041 attgtttttc cagttccgtc attctgctct actcccgtac cagcttcacc gcgttccgcg
     5101 tcttgttgtc caccgtccgg cccgtttcgg ctttcatccc ctccggcagc accagaaacc
     5161 gtccgttgcg gttctccaga taggtcacgc cccacgtctg catgtgcagt ttcgacagcg
     5221 tatctctctg ctgcctgatt tccgccaggt tcgccgctat ccggttcccc tggtaccaca
     5281 gcaccccgct caggctggcg aacagcagcc ctgacaccat ggtgatggtc agccacgtcc
     5341 gcccgaccat tcgcagcacg ctcagccggt tcgactgcat cgccgcgttc atcccctgtt
     5401 cgtgggcgct gatggcgtcg ttgattctct tcgcgcttgc gttcagttcc gatttgacaa
     5461 cgttctcgtg cttgctgaat gcggctttca gcctctcttc ggtaagctgc tgctgtgctt
     5521 tcgatttctc ctcgaagtcc ctcgaggccg ttaaaagact gctcataaat ggctcccctc
     5581 agtcggatat ttcgcccccc gtccgggtcg gcaatgctga tactgttttt tgtggttctg
     5641 accacctcaa aaccggccgc tgtgagcgcc cctgtgacgt catcacgcgt tttcagctct
     5701 cctgatgagg ctaacatcag caaaccccgt gtaatcgtct ctgcggcttc ctgcttcgtt
     5761 tctggcagtc ctgaaggcgt gaccagcgcc cgccggttct ccgggtcgtt cggatcatgt
     5821 agccccagcc tgccgttgac cactgtctgc cacgcatcga tgcgcggccg gtcggcacga
     5881 tcgtaatacg gctggaggcg tttccccgtc agtaattcgg tattggggat cagaaaattc
     5941 agctcaagac gacccttgtc tgcgtgttcc acccacagaa tgctgtactg gtctttatcg
     6001 agtccgggca tcagaacccg ctcaaagctg gccatcagtt tttcacgctg acccggcggc
     6061 aggtccgcct ccgcgaatga cagcaccccg gaggtatatt ttttcacata cggcgacgca
     6121 tcaatcagct cccggacttc ttccggcttc ccctgcagga cgctggcacc ttcacgctgc
     6181 cggtctttac ccagaagata atccacagga ccggcgcccc cgccgcgacc ccggggatgg
     6241 aatttaacaa tcatcgtccg gccctttttc cagtacggca tgccgcaacc gttccagacc
     6301 ggcatcgatc gccatcagcg ctgccacgat ctgcacccgg tcatgaccgg caccaccggc
     6361 gttcactttc cgggcgatct ggttcaggtt attgcccatg ccggcaagct gacgcagcag
     6421 cgcgggcgaa atcgagggaa gcctgccggc gcgtgtcggt ttctcatcaa ggcaggtctg
     6481 gcgcatccac gccgccagtt gttttccgtc gcaccgctca agcaaccgcc ggtgttcgtc
     6541 ttccgtcacc cacatcgtga gcatcttgtt gcgtttatct gccagaatcg cctcctgata
     6601 acgccgtgac ggacgggaaa gcctttcccg gtcggcgcgg ggttggacaa gccgcaggcg
     6661 cgtcagtggg gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata
     6721 ctggcttagt atgttacggc gagatggaga ttcggggaag tgcttcatgt ggcaaggggg
     6781 aaaatacggc tgcggggcgt aagtgcaaaa tgcaggtaac gatgaaatga cgcctcctcg
     6841 ctcactcggt cgctgcgctc ctgccgtgag actgcggcgg gcgttaccag ctcacgaagg
     6901 acgagataac gagatcgcgc tcaagtcagg aaagtcagga aagtcaggaa aaaggatgcg
     6961 gcgttgccgt ttttccatag gctccgcccc cctgacaagc atcacaaaat ctgacgctca
     7021 aatcagtggt ggcgaaaccc gacaggacta taaagatccc aggcgtttcc ccctggtagc
     7081 tccctcgtgc gctctcctgt tcctgccttt cggtttaccg gtgtcattcc gctgttatgg
     7141 ccgcgtttgt ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     7201 ggactgtatg cacgaacccc ccgttcagtc cgactgctgc gccttgtccg gtaactatcg
     7261 tcttgagtcc aacccgaaaa gacatgcaaa agcgccactg gcagcagcca ctggtaatcg
     7321 gattcacgga gcggagtaga gttatctaca gagttcttga agtggtggcc tgactacggc
     7381 tacactgaaa ggacagtttt ggtatctgcg ctccgtgaaa gccagttacc ttcggaagaa
     7441 agagttggta gctctaaatt ccggcaaacg aaccaccgtt ggtagcggtg gtttttttgt
     7501 ttacggctta ggagattacg gctagaacga aaggatctca agaagatcct ctcagaaatt
     7561 atacagacat gcggtagttt gagatgtcag agctatctaa ctcttttacc ggaaccagtt
     7621 tgtaatgcct ttcctcaatc aatttcactt gtccttcgtc tatctttagc tcaaacagtg
     7681 caatcaaatc gctcgaaaca aactgagcgg aaatagctct acatctaaca tttgggtatt
     7741 tttgttcaca ccacgctgta tcttgcgatg tttgcaccat tgatatttga tctgtcccac
     7801 cttttgcttg aactggtatt atataatggc aaccgtattt atctatacct acataaagtt
     7861 catcgatctc tatttgacca attcccttca ccgtagttct taaatgattc tgtaaagaat
     7921 atgtggttat acctagaaat atatctatta gccggttata tctcaccttc gccagcaatg
     7981 cctgctcgtc gtctaaagaa taacctgtaa ttatctccgg ggtattgtct ggaattttta
     8041 ttgccaccat attaggattt ggcaaaatcc tactaatagt tactaatcta aatcgatact
     8101 ttgacctacc agccagctca ataatccact ccttaccttc tggctgagtc aacagaatac
     8161 tttctggcat aggcgttcta aatctgattg aatatataac atcaccaaca ttagatggga
     8221 gttctatacc taaatctttt gcagcactgt ttatatctaa cctttcaaac aaaacctcta
     8281 agtcaccatc aacatattta tcgaaaaata tcttttctac caactgatgg tatctatttt
     8341 tggacctttc ttttttatcc ttactcacaa acaacctcct tgttactatt ttttattctc
     8401 ttagcaatta catttgatga cacaccaaac atagatgcag catcggacat agaaatagca
     8461 accaaacttt catcaccgag tttgattttc ttcgttggtt tttttggcgt aacaccaaga
     8521 accttcataa tctgcaagcc aacacttcta gctagcatag gaggaacact attaccgacc
     8581 tgtctaaatc catgccactt agtcacatga aatctgaacc aatcaggata tgaatgcagt
     8641 cttgctgcct ctctgaccgt gatgacccta ggatagattg gatgtatagg acgaggagat
     8701 gtaaaagcac ctctgtcact tgctgttcct gcccttaaag tattacatag cccattcatc
     8761 gagagctttt taaacctgct gattggttct accgtgtcag gcaaagtttc aacaaaccgc
     8821 cctctagaaa tgtcagagtg attctgtcag taaagaacac gttaatttgt ctttattaaa
     8881 aatccttggg tagctaaaat cactaggatc ttttcctacg ccatgcaaat aggatgagta
     8941 tagtgattgc tcaccaaatt tagcataaac ccaatctcta ccccatagct caggatattt
     9001 ttccgcatca ggaatatccc caatagcgtc cccaaccgtt gttctaatgg ataatggctc
     9061 agggtatgat ggcagagcaa ttcctttcct tccccccatt aagaaaagac gacggcgatc
     9121 ttgaggaact ccataatcag ctgcgtttag cactttataa tcgcttacaa cgtcgtaccc
     9181 gccatcttta aatgcatcca ttatctctac taaaaactgc ttatgcttac caaccgtcaa
     9241 cccttttaca ttttcaaaaa caaagtattt aggctttagc tcaagaacca gccgtacata
     9301 gtgatagacc agtttgtttc tatcatcatc aagcgcgcgt tttcctatta atgaaaagcc
     9361 ttggcaggga gcgcctccga tcactacatc aatctctttc tcaccaatac ctgaaacttc
     9421 cctaacgtat tctccggtaa catcaacaac acttctacat atcgttttgc acataggaaa
     9481 gttatatttg tgagcagagc aatgtattgg atctatttca actgatgcca ttatatcaaa
     9541 gccagcttgc tcgaaaccga gactcagccc acccgctcca gcaaataaat caacagcaat
     9601 cggtctagtc attctttaca cccacgttat ataaaatcaa catgatgcta tcagaaaaac
     9661 gatcgcttta ccgatcgttt ttctgatttc gattgaacat aacatctctt acccgcaccg
     9721 cgccggaggc actcagggcg tttctgatgg ctgacgggca aaaggtggtg gcaagtacac
     9781 cgaaaaccac cagaaacgca cctgatttat tttacgcata tgcatcactt attcacatca
     9841 ctgcatagcc ggtgatttcg gttaaaaaag actctctgtg tgctgaaatc gcagaaaatc
     9901 gccatgatcc ccgtcacgct caggccacct gagacgggcg tttatcagtc tccatactcc
     9961 ctgtcaccat gagataatgt gcgttgccgt tccggtaccg aatctgctgt cttctctggt
    10021 tccttttccg tactttgccc gtatcgctgt tgctcaatct cattcagcct gtcgataacc
    10081 gagcagctgc tcgctcaaac tctgcacctg cctgctcaag cccgacacgc gctcgctcag
    10141 cgccgcgttc tcccgttcca taagcccgga catcttccgc cattcctcga aggcgctctc
    10201 ccactcgtcc agccttctca gtagtcctgc tgtagctgct ctaacgcgct cagcaattgt
    10261 ttttccagtt ccgtcattct gctctactcc cgtcccagct tcaccgcgtt ccgcgtcttg
    10321 ttgtccaccg tccggcccgt ttcggctttc atcccctccg gcagcaccag aaaccgtccg
    10381 ttgcggttct ccagataggt cacgccccac gtctg
//
