LOCUS       NZ_CP141087             8274 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain KE48 plasmid unnamed3, complete sequence.
ACCESSION   NZ_CP141087
VERSION     NZ_CP141087.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38673177
            Assembly: GCF_034643255.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Hogins,J., Do,H.P., Dashti,M., Zimmern,P. and Reitzer,L.
  TITLE     Genome Sequencing of Correlated Pathogenic Escherichia coli and
            Enterococcus Associated with Recurrent Urinary Tract Infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8274)
  AUTHORS   Hogins,J. and Reitzer,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2023) Biological Sciences, The University of Texas
            at Dallas, 800 West Campbell Rd., Richardson, TX 75080, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: OCT-2023
            Assembly Method                   :: other; flye v. 2.9.2
            Assembly Name                     :: KE48
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 96x
            Sequencing Technology :: Illumina NextSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 01:14:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,872
            CDSs (total)                      :: 4,755
            Genes (coding)                    :: 4,543
            CDSs (with protein)               :: 4,543
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 212
            CDSs (without protein)            :: 212
            Pseudo Genes (ambiguous residues) :: 0 of 212
            Pseudo Genes (frameshifted)       :: 87 of 212
            Pseudo Genes (incomplete)         :: 129 of 212
            Pseudo Genes (internal stop)      :: 54 of 212
            Pseudo Genes (multiple problems)  :: 52 of 212
            Pseudo Genes (short protein)      :: 5 of 212
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141087.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="KE48"
                     /isolation_source="Infected Human Urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="USA: Dallas, Texas"
                     /lat_lon="32.77 N 96.79 W"
                     /collection_date="2022-12-19"
                     /collected_by="Jacob Hogins"
                     /subgroup="Group B2"
     gene            complement(join(8116..8274,1..222))
                     /gene="lsoB"
                     /locus_tag="U8P30_RS24315"
                     /old_locus_tag="U8P30_24315"
     CDS             complement(join(8116..8274,1..222))
                     /gene="lsoB"
                     /locus_tag="U8P30_RS24315"
                     /old_locus_tag="U8P30_24315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin LsoB"
                     /protein_id="WP_000710826.1"
                     /translation="MKKDKKYQIEAIKNKDKTLFIVYATDIYSPSEFFSKIESDLKKKK
                     SKGDVFFDLIIPNGGKKDRYVYTSFNGEKFSSYTLNKVTKTDEYNDLSELSASFFKKNF
                     DKINVNLLSKATSFALKKGIPI"
                     /name="lsoB"
     gene            complement(219..1259)
                     /gene="lsoA"
                     /locus_tag="U8P30_RS24320"
                     /old_locus_tag="U8P30_24320"
     CDS             complement(219..1259)
                     /gene="lsoA"
                     /locus_tag="U8P30_RS24320"
                     /old_locus_tag="U8P30_24320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA
                     endoribonuclease LsoA"
                     /protein_id="WP_000068433.1"
                     /translation="MAQNPFKALNINIDKIESALTQNGVTNYSSNVKNERETHISGTYK
                     GIDFLIKLMPSGGNTTIGRASGQNNTYFDEIALIIKENCLYSDTKNFEYTIPKFSDDDR
                     ANLFEFLSEEGITITEDNNNDPNCKHQYIMTTSNGDRVRAKIYKRGSIQFQGKYLQIAS
                     LINDFMCSILNMKEIVEQKNKEFNVDIKKETIESELHSKLPKSIDKIHEDIKKQLSCSL
                     IMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYFTENKPKYIIREI
                     HQETINGEIAEVLCECYTYWHENRHGLFHMKPGIADTKTINKLESIAIIDTVCQLIDGG
                     VARLKL"
                     /name="lsoA"
     gene            complement(1524..1637)
                     /locus_tag="U8P30_RS24325"
                     /old_locus_tag="U8P30_24325"
     CDS             complement(1524..1637)
                     /locus_tag="U8P30_RS24325"
                     /old_locus_tag="U8P30_24325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001459398.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLSPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(2216..5221)
                     /locus_tag="U8P30_RS24330"
                     /old_locus_tag="U8P30_24330"
     CDS             complement(2216..5221)
                     /locus_tag="U8P30_RS24330"
                     /old_locus_tag="U8P30_24330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
                     /protein_id="WP_001143760.1"
                     /translation="MPVDFLTTEQTESYGRFTGEPDELQLARYFHLDEADKEFIGKSRG
                     DHNRLGIALQIGCVRFLGTFLTDMNHIPSGVRHFTARQLGIRDITVLAEYGQRENTRRE
                     HAALIRQHYQYREFAWPWTFRLTRLLYTRSWISNERPGLLFDLATGWLMQHRIILPGAT
                     TLTRLISEVREKATLRLWNKLALIPSAEQRSQLEMLLGPTDCSRLSLLESLKKGPVTIS
                     GPAFNEAIERWKTLNDFGLHAENLSTLPAVRLKNLARYAGMTSVFNIARMSPQKRMAVL
                     VAFVLAWETLALDDALDVLDAMLAVIIRDARKIGQKKRLRSLKDLDKSALALASACSYL
                     LKEETPDESIRAEVFSYIPRQKLAEIITLVREIARPSDDNFHEEMVEQYGRVRRFLPHL
                     LNTVKFSSAPAGVTTLNACDYLSREFSSRRQFFDDAPTEIISRSWKRLVINKEKHITRR
                     GYTLCFLSKLQDSLRRRDVYVTGSNRWGDPRARLLQGADWQANRIKVYRSLGHPTDPQE
                     AIKSLGHQLDSRYRQVAARLCENEAVELDVSGPKPRLTISPLASLDEPDSLKRLSKMIS
                     DLLPPVDLTELLLEINAHTGFADEFFHASEASARVDDLPVSISAVLMAEACNIGLEPLI
                     RSNVPALTRHRLNWTKANYLRAETITSANARLVDFQATLPLAQIWGGGEVASADGMRFV
                     TPVRTINAGPNRKYFGNNRGITWYNFVSDQYSGFHGIVIPGTLRDSIFVLEGLLEQETG
                     LNPTEIMTDTAGASELVFGLFWLLGYQFSPRLADAGASVFWRMDHDADYGVLNDIARGQ
                     SDPRKIVLQWDEMIRTAGSLKLGKVQVSVLVRSLLKSERPSGLTQAIIEVGRINKTLYL
                     LNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQEDQLGTLGLVTNA
                     VVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYSFTLAELVTKGHLR
                     PLKEASEAENVA"
                     /name="Tn3-like element Tn3 family transposase"
     gene            5385..5942
                     /locus_tag="U8P30_RS24335"
                     /old_locus_tag="U8P30_24335"
     CDS             5385..5942
                     /locus_tag="U8P30_RS24335"
                     /old_locus_tag="U8P30_24335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221001.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001235713.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTDR
                     EGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGDMG
                     QMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTGATE
                     IAHQLSIARSTVYKILEDERAS"
                     /name="recombinase family protein"
     gene            6125..6985
                     /locus_tag="U8P30_RS24340"
                     /old_locus_tag="U8P30_24340"
     CDS             6125..6985
                     /locus_tag="U8P30_RS24340"
                     /old_locus_tag="U8P30_24340"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            complement(7124..7273)
                     /locus_tag="U8P30_RS24345"
                     /old_locus_tag="U8P30_24345"
     CDS             complement(7124..7273)
                     /locus_tag="U8P30_RS24345"
                     /old_locus_tag="U8P30_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001677812.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EEGV"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7558..7881
                     /gene="mobA"
                     /locus_tag="U8P30_RS24350"
                     /old_locus_tag="U8P30_24350"
     CDS             7558..7881
                     /gene="mobA"
                     /locus_tag="U8P30_RS24350"
                     /old_locus_tag="U8P30_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_000166966.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     ARG             6126..6982
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7309..7549
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.12e-123"
     Typing          complement(1566..2095)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.972"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          7559..7878
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="2.19e-62"
ORIGIN
        1 cttctcgcca ttaaatgacg tatagacgta acgatctttt tttccaccgt taggaattat
       61 taaatcaaaa aaaacatcac ccttgctttt ctttttcttc aagtcggatt cgatttttga
      121 gaaaaattcg ctcgggctat aaatatcagt agcatagaca ataaataaag ttttatcttt
      181 attttttatt gcttctattt gatatttttt atcttttttc ataatttcaa cctagctacg
      241 ccaccatcta ttaattggca aacggtatcg atgattgcga ttgattctaa tttgttaatt
      301 gtcttcgtgt cagctattcc tggtttcata tgaaacaaac catgcctgtt ctcatgccag
      361 taagtgtagc attcacacaa aacttccgct atttcaccat ttatagtttc ttggtgtatt
      421 tctctgatta tatatttggg tttgttttca gtgaagtatt cgccaaggtt ctttgatgat
      481 gatggattgc aaacatcatt aagtatttga tatataaagc cttctatggc tcttaatgca
      541 gagaagcagt atgttgagta atcttccatt tcgacatcta tttttttcat tattagcgag
      601 catgatagct gttttttgat atcttcatgg attttatcga tgctttttgg tagtttgcta
      661 tgcaactcgg actcaatagt ttcttttttt atgtcaacat taaattcttt atttttttgt
      721 tcgacaatct ctttcatgtt tagtattgag cacatgaaat cgttaatcaa actcgcgatt
      781 tgaaggtatt ttccttggaa ttgaatagag ccgcgcttgt aaatttttgc cctgaccctg
      841 tcaccattgc tggtggtcat aatatattgg tgtttacaat taggatcgtt attattatct
      901 tctgttattg ttatcccctc ttcagaaaga aattcaaata gatttgccct gtcatcatca
      961 ctgaattttg gaatggtgta ttcaaagttc tttgtgtctg aatacaaaca gttttctttt
     1021 ataatcaagg cgatttcatc aaagtaagtg ttattttgcc cagacgctct tccgatagtg
     1081 gtatttccgc ctgatggcat tagttttatt aagaagtcta ttcctttata tgtgccagat
     1141 atgtgagttt ctctttcgtt ttttacatta gaggaatagt ttgtgacgcc attctgcgtc
     1201 agagcagact caatcttgtc aatattgata tttagtgctt taaacgggtt ctgtgccatt
     1261 gggtcaatcc gttgtttttt ttgaatatgt acagatcttg tttttttgtc aacggaatag
     1321 ctgttcgttg acttgataga ccgattgatt catcatctca taaataaaga aaaaccaccg
     1381 ctaccaacgg tggttttctc aaggttcgct gagctaccaa ctctttgaac caaggtaact
     1441 ggcttggagg agcgcagtca ccaaaatctg ttctttcagt ttagccttaa caggtgcata
     1501 acttcaagac aaactcctct aaatcagtta ccaatggctg ctgccagtgg cgataagtcg
     1561 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     1621 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     1681 caacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     1741 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     1801 tggtatcttt atagtcctgt cgggtttcgc cacctctggc ttgagcgtcg atttttgtga
     1861 tgctcgtcag gggggcggag cctatggaaa aacgcctgcg gcgttggctt cttccggtgc
     1921 tttgcttttt gctcacatgt tctttccggc tttatcccct gattctgtgg ataaccgtat
     1981 taccgctttt gagtgagctg acaccgctcg ccgcagtcga acgaccgagc gtagcgagtc
     2041 agtgagcgag gaagcggaag agcgccttat gtgacatttt ctccttacgc tctgttgtgc
     2101 cgttcggcat cctgtcctga gagttatctc tctgtgctat tttctacttc aaagcgtgtc
     2161 tggatgctgt tctggagttc ttggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2221 caacgttttc tgcctctgac gcctctttta atggtctcag atgtcctttg gtcaccagtt
     2281 ctgccagcgt gaaggaataa tggccgagca tattgatatg tccgtggcaa agcggggaga
     2341 ggcgtgcgat atcttcatca ttcagtgttt caccctgcgc ccggagatga tccagggctg
     2401 cctgcatata aatagtgttc cataacacga cggcgttagt gaccagcccc agtgtgccca
     2461 gttgatcttc ctgaccgtcg gtatatcgtt ttcttatctc acctttttga ccgtgacaga
     2521 tggctctggc aacggcatgg cgactttctc cccgattaag ctgggtcaga atgcgccggc
     2581 ggtaatcttc atcatcaata taattaagca gatacagcgt tttgttgatg cgccccactt
     2641 caatgattgc ctgagtcagt ccggaaggac gttcactttt cagcaatgaa cggaccagca
     2701 ctgaaacctg tactttgccc agcttcaggg agccagcggt ccggatcatt tcgtcccact
     2761 gaaggactat ttttcgggga tctgattgcc ctctggcaat atcattcagc acgccatagt
     2821 cggcatcatg gtccattcgc cagaaaaccg aagcaccggc atcagccagg cgtggagaaa
     2881 actggtatcc cagcagccag aaaaggccaa agacaagttc gctggcacct gctgtatcgg
     2941 tcataatttc ggttggattc agcccggtct cctgttccag aagaccttcc agcacaaaga
     3001 tagagtccct cagcgtcccc ggtataacga tgccatgaaa gccggaatac tgatcggaca
     3061 caaagttgta ccaggtgatc cctctgttat taccaaagta tttgcggttc ggtccggcat
     3121 tgattgttct gactggcgta acaaagcgca ttccatctgc agatgccact tctcctccac
     3181 cccatatctg tgccagtggc agcgttgcct gaaaatcaac cagtctggca ttagcgctgg
     3241 tgatagtttc agcccgcaga tagttcgctt ttgtccagtt cagccggtgt cgggtcagtg
     3301 caggaacatt tgatctgatc agtggttcca gaccgatatt gcaggcttca gccatcagca
     3361 cggcgctgat gctgacgggc agatcatcaa ctctggcact ggcttcacta gcatggaaaa
     3421 actcatcagc aaatccggta tgggcgttaa tttcgagcag caactccgtt aaatccaccg
     3481 gagggagtag atcactgatc attttgctca gtcgtttcag actgtccggc tcatcaagac
     3541 tggcgagggg agaaattgtc aaccggggct tcgggccaga aacatcgagt tcgacagcct
     3601 cattttcgca aagacgtgca gcaacctgtc tgtaacgact atcaagctga tgacccagag
     3661 attttattgc ttcctgcggg tctgtcgggt gccccaaaga acgataaacc ttaatccggt
     3721 ttgcctgcca gtcagcaccc tgtagtaatc ttgcacgagg atctccccac cggttactgc
     3781 cggtaacgta gacatccctc cgcctcagac tatcctgcag tttactgaga aagcagagcg
     3841 tgtatcccct gcgggtgata tgtttttcct tgttaatcac cagccgtttc catgaccgac
     3901 tgataatttc cgttggtgcg tcgtcaaaaa actgccgccg tgagctgaac tcccggctga
     3961 ggtagtcaca ggcattcaga gtggtaaccc cggcaggtgc ggatgaaaat ttaacggtat
     4021 tcagcagatg gggcaggaaa cgacgaacgc gcccgtactg ctccaccatt tcttcatgaa
     4081 aattatcgtc tgagggccgg gcaatttcac ggacaagcgt gatgatttca gccagctttt
     4141 gccttgggat gtagctgaac acctcagcac gaatcgattc gtccggtgtt tcttctttca
     4201 gcaggtacga acatgcgctg gcgagcgcca atgcagattt atccagatcc ttcagcgagc
     4261 ggagccgttt tttctgccca atctttctgg cgtcacggat gataacggcc agcatggcgt
     4321 ccagaacgtc caatgcatca tccagcgcca gcgtttccca tgcaaggaca aaggcaacca
     4381 gaaccgccat ccttttctgc ggtgacatcc tggcaatatt gaacaccgaa gtcataccag
     4441 cataacgtgc gagatttttc aggcgcacag ccgggagtgt actcaggttt tcagcatgca
     4501 ggccaaaatc gttcagagtt ttccagcgtt caattgcttc attaaacgcc ggaccactga
     4561 tggtcacagg gccctttttc agtgattcca gtaaagacag gcggctgcaa tcagttggcc
     4621 ccagcagcat ctccagctgt gaacgctgtt cggctgacgg tatcagtgcc agtttgttcc
     4681 acaggcgcaa cgtcgccttt tcccttacct ctgaaatcaa ccgggtcagc gtagtggctc
     4741 cggggagaat aatacgatgt tgcataagcc accctgtcgc cagatcgaaa agcaggccag
     4801 gacgttcgtt gcttatccag ctccgggtat ataaaagacg ggtaaggcga aatgtccagg
     4861 gccaggcaaa ttcacgatac tgatagtgct gacgtatcag cgctgcatgc tcacggcggg
     4921 tattttccct ctgaccgtat tctgcaagaa cggtgatatc acgaatcccg agctgtctgg
     4981 cggtaaaatg ccggacgccg gaaggaatat gattcatatc ggtgaggaag gtgcccagaa
     5041 aacggacaca tccaatttgc agggcaatgc ccagacggtt gtgatcacct ctgctttttc
     5101 cgataaattc cttgtctgct tcatcaaggt gaaaatatcg tgccagctga agctcatccg
     5161 gttcaccggt gaatctgcca tagctttcag tctgctcagt ggtcagaaag tcaacgggca
     5221 tatcggcctc cctgcctgac gggcatttag taacattttt ccaaccgtac gaaatgttat
     5281 aaattatcgg acatcgtaaa actgttacat taatatgtct attaaatcgt aaatttgtaa
     5341 taatagacat gagttgtccg atattcgatt taaggtacat ttttatgcga ctttttggtt
     5401 acgctcgggt ctcaaccagt cagcagtctc ttgatcttca ggtcagagca ctcaaagacg
     5461 caggtgtgaa agcaaaccgt atatttaccg ataaggcatc cggcagttca acagaccggg
     5521 aagggctgga tttgctgagg atgaaggtgg aggaaggtga tgtcattctg gttaagaagc
     5581 tcgaccgtct tggccgcgac actgccgata tgatccaact gataaaggaa tttgacgctc
     5641 agggcgtggc agtccggttc attgatgacg ggatcagtac cgacggtgat atggggcaaa
     5701 tggtggtcac catcctgtcg gctgtggcac aggctgaacg ccggaggatc ctagaacgca
     5761 cgaatgaggg ccgacaggaa gcaaagctga aaggaatcaa atttggccgc aggcgtaccg
     5821 tggacaggaa cgtcgtgctg acgcttcatc agaagggcac tggtgcaacg gaaattgctc
     5881 atcagctcag tattgcccgc tccacggttt ataaaattct tgaagacgaa agggcctcgt
     5941 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     6001 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     6061 tatgtatccg ctcatgagac aataaccctg gtaaatgctt caataatatt gaaaaaggaa
     6121 gagtatgagt attcaacatt ttcgtgtcgc ccttattccc ttttttgcgg cattttgcct
     6181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     6241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     6301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gtgcggtatt
     6361 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     6421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     6481 attatgcagt gctgccataa ccatgagtga taacactgct gccaacttac ttctgacaac
     6541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     6601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     6661 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     6721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     6781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     6841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     6901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     6961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     7021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     7081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccttcttctg
     7141 cgagttcgtg cagcttctca cacatggcag cctgttcgtc agcatcgagt gcgtccagct
     7201 tttcgagcag cgtcaggctc tggcttttta tgaatcctgc catgttgagt acagcttgct
     7261 gctgcttgtt catcttttcg ttttctccgt tctgtctgtc atctgcgttg tgtgattata
     7321 tcgcgtacca cttttcgact gttttaccgc cggtattctg ccgtctgacg ctttgacggg
     7381 tatttctgcc tgacaatact gtcactgcca aaaaactgcc gtgcctttgt cggtaattcg
     7441 agcttgctga caggacagga tgtgcaattg ttataccgcg catacatgca cgctattaca
     7501 attaccctgg tcagggcttc gccccgacac cccatgtcag atacggagcc atgttttatg
     7561 acaaaacgaa gtggaagtaa tacgcgcagg cgggctatca gtcgccctgt tcgtctgacg
     7621 gcagaagaag accaggaaat cagaaaaagg gctgctgaat gcggcaagac cgtttctggt
     7681 tttttacggg cggcagctct cggtaagaaa gttaactcac tgactgatga ccgggtgctg
     7741 aaagaagtta tgcgactggg ggcgttgcag aaaaaactct ttatcgacgg caagcgtgtc
     7801 ggggacagag agtatgcgga ggtgctgatc gctattacgg agtatcaccg tgccctgtta
     7861 tccaggctta tggcagatta acttcccgga gagaaactgt cgaaaacaga cggtatgaac
     7921 gccgtaagcc cccaaaccga tcgccattca ctttcatgca tagctatgca gtgagctgaa
     7981 agcgatcctg acgcattttt ccggtttacc ccggggaaaa catctctttt tgcggtgtct
     8041 gcgtcagaat cgcgttcagc gcgttttggc ggtgcgcgta atgagacgtt atggtaaatg
     8101 tcttctggct tgatattata ttggaatgcc ttttttcaaa gcaaatgatg tggctttgga
     8161 tagaaggttt acgttgatct tatcaaagtt ttttttaaag aacgaagccg agagctcaga
     8221 taaatcatta tattcatcag ttttcgtaac tttgtttaat gtgtaacttg aaaa
//
