LOCUS       NZ_CP141066            11137 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KPN7 plasmid p1, complete sequence.
ACCESSION   NZ_CP141066
VERSION     NZ_CP141066.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38415150
            Assembly: GCF_034661255.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11137)
  AUTHORS   Zhang,W.
  TITLE     Resistance and virulence analysis of hv-CRKP,KPN7
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11137)
  AUTHORS   Zhang,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-DEC-2023) Department of Clinical Laboratory, Shanghai
            University of Medicine and Health Sciences Affiliated Zhoupu
            Hospital, No.1500, Zhouyan Rd., PuDong New District, Shanghai
            201318, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: ABySS v. 2.0.2; Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 233.0x
            Sequencing Technology             :: PacBio; Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 04:00:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,106
            CDSs (total)                      :: 5,987
            Genes (coding)                    :: 5,786
            CDSs (with protein)               :: 5,786
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 8, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 8, 7 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 201
            CDSs (without protein)            :: 201
            Pseudo Genes (ambiguous residues) :: 0 of 201
            Pseudo Genes (frameshifted)       :: 69 of 201
            Pseudo Genes (incomplete)         :: 141 of 201
            Pseudo Genes (internal stop)      :: 24 of 201
            Pseudo Genes (multiple problems)  :: 33 of 201
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP141066.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11137
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KPN7"
                     /db_xref="taxon:573"
                     /plasmid="p1"
                     /geo_loc_name="China:Shanghai"
                     /collection_date="2023-09"
     gene            1..327
                     /locus_tag="SM752_RS27415"
                     /old_locus_tag="SM752_27410"
     CDS             1..327
                     /locus_tag="SM752_RS27415"
                     /old_locus_tag="SM752_27410"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            353..898
                     /locus_tag="SM752_RS27420"
                     /old_locus_tag="SM752_27415"
     CDS             353..898
                     /locus_tag="SM752_RS27420"
                     /old_locus_tag="SM752_27415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            964..1314
                     /locus_tag="SM752_RS27425"
                     /old_locus_tag="SM752_27420"
     CDS             964..1314
                     /locus_tag="SM752_RS27425"
                     /old_locus_tag="SM752_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(1384..1650)
                     /locus_tag="SM752_RS27430"
                     /old_locus_tag="SM752_27425"
     CDS             complement(1384..1650)
                     /locus_tag="SM752_RS27430"
                     /old_locus_tag="SM752_27425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1634..1858)
                     /locus_tag="SM752_RS27435"
                     /old_locus_tag="SM752_27430"
     CDS             complement(1634..1858)
                     /locus_tag="SM752_RS27435"
                     /old_locus_tag="SM752_27430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(2525..2737)
                     /locus_tag="SM752_RS27440"
                     /old_locus_tag="SM752_27435"
     CDS             complement(2525..2737)
                     /locus_tag="SM752_RS27440"
                     /old_locus_tag="SM752_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2744..3253)
                     /locus_tag="SM752_RS27445"
                     /old_locus_tag="SM752_27440"
     CDS             complement(2744..3253)
                     /locus_tag="SM752_RS27445"
                     /old_locus_tag="SM752_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3288..3992)
                     /locus_tag="SM752_RS27450"
                     /old_locus_tag="SM752_27445"
                     /pseudo=""
     CDS             complement(<3288..3992)
                     /locus_tag="SM752_RS27450"
                     /old_locus_tag="SM752_27445"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3931..4275)
                     /locus_tag="SM752_RS27455"
                     /old_locus_tag="SM752_27450"
     CDS             complement(3931..4275)
                     /locus_tag="SM752_RS27455"
                     /old_locus_tag="SM752_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(4265..4441)
                     /locus_tag="SM752_RS27460"
                     /old_locus_tag="SM752_27455"
     CDS             complement(4265..4441)
                     /locus_tag="SM752_RS27460"
                     /old_locus_tag="SM752_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            5081..5239
                     /locus_tag="SM752_RS27465"
                     /old_locus_tag="SM752_27460"
     CDS             5081..5239
                     /locus_tag="SM752_RS27465"
                     /old_locus_tag="SM752_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            5894..6439
                     /locus_tag="SM752_RS27470"
                     /old_locus_tag="SM752_27465"
     CDS             5894..6439
                     /locus_tag="SM752_RS27470"
                     /old_locus_tag="SM752_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            6505..6855
                     /locus_tag="SM752_RS27475"
                     /old_locus_tag="SM752_27470"
     CDS             6505..6855
                     /locus_tag="SM752_RS27475"
                     /old_locus_tag="SM752_27470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(6925..7191)
                     /locus_tag="SM752_RS27480"
                     /old_locus_tag="SM752_27475"
     CDS             complement(6925..7191)
                     /locus_tag="SM752_RS27480"
                     /old_locus_tag="SM752_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7175..7399)
                     /locus_tag="SM752_RS27485"
                     /old_locus_tag="SM752_27480"
     CDS             complement(7175..7399)
                     /locus_tag="SM752_RS27485"
                     /old_locus_tag="SM752_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(8066..8278)
                     /locus_tag="SM752_RS27490"
                     /old_locus_tag="SM752_27485"
     CDS             complement(8066..8278)
                     /locus_tag="SM752_RS27490"
                     /old_locus_tag="SM752_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(8285..8794)
                     /locus_tag="SM752_RS27495"
                     /old_locus_tag="SM752_27490"
     CDS             complement(8285..8794)
                     /locus_tag="SM752_RS27495"
                     /old_locus_tag="SM752_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<8829..9533)
                     /locus_tag="SM752_RS27500"
                     /old_locus_tag="SM752_27495"
                     /pseudo=""
     CDS             complement(<8829..9533)
                     /locus_tag="SM752_RS27500"
                     /old_locus_tag="SM752_27495"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(9472..9816)
                     /locus_tag="SM752_RS27505"
                     /old_locus_tag="SM752_27500"
     CDS             complement(9472..9816)
                     /locus_tag="SM752_RS27505"
                     /old_locus_tag="SM752_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(9806..9982)
                     /locus_tag="SM752_RS27510"
                     /old_locus_tag="SM752_27505"
     CDS             complement(9806..9982)
                     /locus_tag="SM752_RS27510"
                     /old_locus_tag="SM752_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            10622..10780
                     /locus_tag="SM752_RS27515"
                     /old_locus_tag="SM752_27510"
     CDS             10622..10780
                     /locus_tag="SM752_RS27515"
                     /old_locus_tag="SM752_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     Typing          complement(4528..4604)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          complement(10069..10145)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          4793..5190
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          10334..10731
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(2410..3941)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7951..9482)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagaaa gaacaaatat catgctagat ataggattaa agcaatcctt acaacggctt
       61 tcgaataaaa ccaaaatgag tctaagtgat cttattaacc agagattatc aggttcactt
      121 tcagttgaag aacgcattgg cgaaaagcct gaatggatga gaaaccttga tccctatctc
      181 gccaatcact tttcaaatat tgagttaccg ctcccttcac cattaaaaga ataccaagaa
      241 aaaatggatg agatagattc aattattaat gacatgatgg ttctaaagga atatttcaga
      301 gctaagtttt acgacgaaat aaaataggca cgctatacgg aggcggagaa ttatgttttt
      361 taaacggtat ttaacggaga aggaaatata caaggcagca ctcccaattg ttaggcttca
      421 agggagagtt aacacttcat tacgtttttt atccaaatac tcagatgtaa acaaaaatgg
      481 attaattgca ttgcatctta atttattaca ttttatctca tacaacatac acggtgtaga
      541 gtctctgaaa atcactcaag caggcataaa caaagctgta caacaaagcc ctcacgcaga
      601 tgaaataaaa aatattgcgc aggcaatagg acgcaaagga cctgagtaca tcgaaggatt
      661 aattagaaac ccatggggga agaaaaatga taatccacta atatttataa tgagcattga
      721 agaaagtata aaagaaaccc ttaactcagg ttatatgtta tatagagtag ctcccataac
      781 tgagggtgaa gcagctgctt tcatggatgt tcttaaacaa ttattattat ttgaggataa
      841 ttttcgcctt tttggtagag acttggttta taaaatagaa aaattaaaaa tcagataaca
      901 aagtatggtg aacgaaaaat gaaagagagg atagtgaaat gtgtaatacc aagattctca
      961 actatgtatt ggttaattgc gttcctagca tttatattcc ccggtcgatc ttttgctgac
     1021 ggttacaccg cttactaccg atgtaatggt actcaggaag catctcttac tgttaaagat
     1081 aacaaactaa gtatggttgt aggtctttct aagtttaata ggcaactgga tacttataga
     1141 ttcaaagaaa agggaatcga agtagtaatg cagagagctg cttctgatga taactctgag
     1201 gccataatgt cttttattat gcctgacgtt aacaatatga ccttattagt taaaaataac
     1261 ggctacatta tcgttaagac aacctgctgg ttaactaaaa ccgacaaaca gtagaacacg
     1321 tagttagtcg agtatttacc ataatacctg ttgtcggcat caaaaaagcc cgcatagcgg
     1381 gcttcagtcg taaacttcgc ggcgatgtcc aatcgtggcg gcaaggataa caagctcgtc
     1441 atctcggatc tcacacagaa cgcgataatc tcccacccga tagcgccaga actcgcccag
     1501 ctcacctttg agcggcttcc ctgactggcg aggatcggca gcaactgcaa tgcgtaaact
     1561 cataaaatcc acaatccgtc gtgcgttctg tttgtccatc ttgcgtaacg atttgagcgc
     1621 ccgatcggaa tagttaatcg tccagaccaa gtgacttcct cacgtcttca gacgaatgca
     1681 ctgcttcatc accacggcga acgcgtgcag cagtttctgc tgaaagataa taatcctcca
     1741 ggtcttccag atgcgccagt attgcttccc gcgcataaaa cgtctttgtt ctgccggttt
     1801 gcttcgccag cgagtccaga cgggactcaa tctcatcaga taaccggata gcaagcatat
     1861 aaacctcccg aacaccattt gctatacatg tatatcaaca gtaccacaat caacctaaaa
     1921 gaacaaaaaa taatcttgtt agcccctctt gataatccgt aacgcggtca atatatctag
     1981 cttgagggct ttcctcttaa ctattgaaag gataacgatt atgggtctta agccgggtcc
     2041 gaaacgaatt gccgattcaa caggggaacc tgataaacgc cagcgtgaca ataaaaaaac
     2101 gccgggaaat actgacaaat tgaaacccag caaatcttca aaaaaataag tcagtagtta
     2161 aaagccctcc ggggcttttt tgtatctgcc gttcacgctg attaaacata acacccgttg
     2221 cccgcaccgc cccggacggg ttcgccgcga acggggtggc agtcaggtaa aaccggcaac
     2281 aaaccacccg ataaaccgcc ccgttcgcga cagataaatt ttacctggct gcatagccat
     2341 gcagccaggt gaatgacatc cggtttcagc gtaaatgccc gatgagaacc ctgttttcct
     2401 gcatttcagc ccagcgacgg accgtcatag cttcgctgcc gggaacgcgc ttccgtcagt
     2461 tccttttcgt gctgccgttg ctcgtgcgcc cgttctgcgg ccacggccat ctcatgacgc
     2521 tgcactaccg gctcaaacgc tgcagttgcc cgctcaagcg ttccacctgc tgactcaagc
     2581 gcgtcacgcg ctcgctcagc gccgcgttct cccgttgcat aagccctgac attttccgcc
     2641 attccccgaa ggcgctctcc cactcgtcca gccttttcga gtagtcctgc tgtagctgct
     2701 ctaatgcgct cagcaactgt ttttccagct cggtcatcgg cacttactcc ttcaccagtc
     2761 tcagcgcgtt ctgcttcgac cgcccgttcc cgaccgtcca gctctgcgtc ctgtctacgg
     2821 ccgtcccctt cggcagcacc agaaaacgtc cgttgctgtc ttccaggtac gtcacgcccc
     2881 aggtcttcgc gttcagcttc gacagcgctg cgttctgccg gtcgatttcg gccagatttg
     2941 acgctatcaa gctcccctga taccacaaca ccccgctcag gctggcgaac agcagtcctg
     3001 acaccatcgt gatggtcagc catgtccgcc cgaccatacg catcacgctc aggcggttcg
     3061 actgcatggc cgcgctcata ccctgctcgt gggcgcggat ggcggcgctg attctcttcc
     3121 cgctttcgct cagttccgct ctgacagact tctcgtgctc gctgaatgcg gctttcagca
     3181 tttcgccggt actctgctgc tgcgctttcg atttctgctc taagtccttc gccagcgtta
     3241 aaagactgtt catagatggc tcccttaagt cggatgtttc gccctccgtc cgggtcggca
     3301 atgctgatgc tgctctttgt ggtgcgcacg acctcaaaac ctgcgctttc cagcgcctca
     3361 gtgacgtcct gacgcgtttt aagctcccct gacgaggcga gggccagtaa gccccgcgta
     3421 atagcctctg cggcttcctg cttcgttttg ggtaacgaag atggcgtcac cagcgcgcgc
     3481 cggttctccg gtgcgttcgg gtcgtgcagc cccagtcgcc cgttcaccac ggtctgccac
     3541 gcatcgatgc gcggacggtc tgcccggtcg tagtacggct ggagacgcct gccggtcagc
     3601 agttccgtgt tcggtaccag aaaattcagt tccagccgcc ccttgtcccg gtgttcaacc
     3661 cacagcacgc tgtactggtc tttatcgagt ccgggcatca gaacccgctc gaagctcgcc
     3721 atcagctttt cacgctggcc gggcggtaaa tcctgttcag caaaagacag gacgccggag
     3781 gtgtactttt tggcgtaggg cgaggcgtcg ataagctccc ggacctcctc cggctttccc
     3841 tgcaggacga tggccccgtc gcgctggcgg tctttcccca gcagataatc caccggcccg
     3901 gcaccgccgc ctcgccctcg cggatgaaac ttaacgatca tcgtctgccc ccttttccag
     3961 cacggcatgc cgcagccgct cgagttcggc atcaatggcc atcagcgcgg ccaccacctg
     4021 aacccggtca gcgcctgacc actggccgcc gttaattttc cgggcgatct ggttgaggtt
     4081 gttccccatc ccggcaagct ggcgcagcaa aaccggatcg atggacggca gccgcaacga
     4141 ccgcgccggg cgcgtatcga gacaggtttc ccgcatccac accgccagtt gccttccatt
     4201 gcagcgctcc agcagctgct ggtactcccc atcggtcacc cggataccta aaaaacgggt
     4261 acgcttaacc gccatcctct cctccgatat ccgcaaaatg tgaccgggct gaacgctcaa
     4321 gctctgccat ctgctcccgg cggtaatctt cctcctgtgc ctgacgcatc agatttgcca
     4381 tcactgccgg atgccaggat ctttttttta cgacgagcgg cttcttcttc ggcttctgca
     4441 ttatccctcc ttcgcaaacg ccggacggca cggaaaagtt ttccgggtcg ctccggcgtt
     4501 ggacaagcgc cagcgcgtca gtggtgttag cgggtttcgg ggcgcagccc tgaaccagtc
     4561 acagcgttta cgctgtgtat actggcttac tatgttggca tgagagcacg attcatgaaa
     4621 gtgcctcatg tggcaggtgt gatatgtggc tcaggagcgt ccgcaatacc atgcacggac
     4681 agatgaaatg acgcctcctc gctcactcgg tagctgcgct cctgccgttc ggctgcggcg
     4741 ggcgtgaccg actcacggat aacgggataa agcaaaggcg ctcaaatcag aaagggccgg
     4801 agaaaaggat gcggcgttgc cgtttttcca taggctccgc ctcccctgac gagcatcaca
     4861 gaaatggacg ctcaagtcag aggaggacga aacccgacag gacttaaaga tgccggtgat
     4921 ttctctccgg cggctccctc gtgcgctctc ctgttccgac cctgccgttt accggatacc
     4981 tgttccgcct ttttcccttt cgggaagcgt ggcgctttct catagctcac gctgctggta
     5041 tctcggctcg gtttaggtcg ttcgctccaa gctgggctgt atgcacgaac cccccgttca
     5101 gcccgactgc tgcgcccttt ccggtaacta tcatcttgag tccaacccgg acggacacgc
     5161 aaaaacgcca ctggcagcag ccattggtaa ctggatatat acgagagaga tattgagagg
     5221 tcttgaagtg ggtgcctaac tgcggctaca ctgaaagaac agttttggta tctgcggtct
     5281 cgtataccca gttacccggt taagcagttc cccaactgac ttaaccttcg atcaaaccgc
     5341 ctccccaggc ggttttttcg tttacagtgc aagagattac gcgcagaaaa aaaggatctc
     5401 aagaagatcc tttaaatgat cgtcagttct gttatccccg gagctctatc catcccacgg
     5461 aaaaacaccc gccactgatg ccctcagcgc actgcacatc atgtacaaac cactccctcc
     5521 ctcctttttt attgcgtttc ttaatgcgca atggtagctt tgtgcgcaac aaaaaacgca
     5581 atagcaaggg ggggtaatga gagaaagaac aaatatcatg ctagatatag gattaaagca
     5641 atccttacaa cggctttcga ataaaaccaa aatgagtcta aggaaaagcc tgaatggatg
     5701 agaaaccttg atccctatct cgccaatcac ttttcaaata ttgagttacc gctcccttca
     5761 ccattaaaag aataccaaga aaaaatggat gagatagatt caattattaa tgacatgatg
     5821 gttctaaagg aatatttcag agctaagttt tacgacgaaa taaaataggc acgctatacg
     5881 gaggcggaga attatgtttt ttaaacggta tttaacggag aaggaaatat acaaggcagc
     5941 actcccaatt gttaggcttc aagggagagt taacacttca ttacgttttt tatccaaata
     6001 ctcagatgta aacaaaaatg gattaattgc attgcatctt aatttattac attttatctc
     6061 atacaacata cacggtgtag agtctctgaa aatcactcaa gcaggcataa acaaagctgt
     6121 acaacaaagc cctcacgcag atgaaataaa aaatattgcg caggcaatag gacgcaaagg
     6181 acctgagtac atcgaaggat taattagaaa cccatggggg aagaaaaatg ataatccact
     6241 aatatttata atgagcattg aagaaagtat aaaagaaacc cttaactcag gttatatgtt
     6301 atatagagta gctcccataa ctgagggtga agcagctgct ttcatggatg ttcttaaaca
     6361 attattatta tttgaggata attttcgcct ttttggtaga gacttggttt ataaaataga
     6421 aaaattaaaa atcagataac aaagtatggt gaacgaaaaa tgaaagagag gatagtgaaa
     6481 tgtgtaatac caagattctc aactatgtat tggttaattg cgttcctagc atttatattc
     6541 cccggtcgat cttttgctga cggttacacc gcttactacc gatgtaatgg tactcaggaa
     6601 gcatctctta ctgttaaaga taacaaacta agtatggttg taggtctttc taagtttaat
     6661 aggcaactgg atacttatag attcaaagaa aagggaatcg aagtagtaat gcagagagct
     6721 gcttctgatg ataactctga ggccataatg tcttttatta tgcctgacgt taacaatatg
     6781 accttattag ttaaaaataa cggctacatt atcgttaaga caacctgctg gttaactaaa
     6841 accgacaaac agtagaacac gtagttagtc gagtatttac cataatacct gttgtcggca
     6901 tcaaaaaagc ccgcatagcg ggcttcagtc gtaaacttcg cggcgatgtc caatcgtggc
     6961 ggcaaggata acaagctcgt catctcggat ctcacacaga acgcgataat ctcccacccg
     7021 atagcgccag aactcgccca gctcaccttt gagcggcttc cctgactggc gaggatcggc
     7081 agcaactgca atgcgtaaac tcataaaatc cacaatccgt cgtgcgttct gtttgtccat
     7141 cttgcgtaac gatttgagcg cccgatcgga atagttaatc gtccagacca agtgacttcc
     7201 tcacgtcttc agacgaatgc actgcttcat caccacggcg aacgcgtgca gcagtttctg
     7261 ctgaaagata ataatcctcc aggtcttcca gatgcgccag tattgcttcc cgcgcataaa
     7321 acgtctttgt tctgccggtt tgcttcgcca gcgagtccag acgggactca atctcatcag
     7381 ataaccggat agcaagcata taaacctccc gaacaccatt tgctatacat gtatatcaac
     7441 agtaccacaa tcaacctaaa agaacaaaaa ataatcttgt tagcccctct tgataatccg
     7501 taacgcggtc aatatatcta gcttgagggc tttcctctta actattgaaa ggataacgat
     7561 tatgggtctt aagccgggtc cgaaacgaat tgccgattca acaggggaac ctgataaacg
     7621 ccagcgtgac aataaaaaaa cgccgggaaa tactgacaaa ttgaaaccca gcaaatcttc
     7681 aaaaaaataa gtcagtagtt aaaagccctc cggggctttt ttgtatctgc cgttcacgct
     7741 gattaaacat aacacccgtt gcccgcaccg ccccggacgg gttcgccgcg aacggggtgg
     7801 cagtcaggta aaaccggcaa caaaccaccc gataaaccgc cccgttcgcg acagataaat
     7861 tttacctggc tgcatagcca tgcagccagg tgaatgacat ccggtttcag cgtaaatgcc
     7921 cgatgagaac cctgttttcc tgcatttcag cccagcgacg gaccgtcata gcttcgctgc
     7981 cgggaacgcg cttccgtcag ttccttttcg tgctgccgtt gctcgtgcgc ccgttctgcg
     8041 gccacggcca tctcatgacg ctgcactacc ggctcaaacg ctgcagttgc ccgctcaagc
     8101 gttccacctg ctgactcaag cgcgtcacgc gctcgctcag cgccgcgttc tcccgttgca
     8161 taagccctga cattttccgc cattccccga aggcgctctc ccactcgtcc agccttttcg
     8221 agtagtcctg ctgtagctgc tctaatgcgc tcagcaactg tttttccagc tcggtcatcg
     8281 gcacttactc cttcaccagt ctcagcgcgt tctgcttcga ccgcccgttc ccgaccgtcc
     8341 agctctgcgt cctgtctacg gccgtcccct tcggcagcac cagaaaacgt ccgttgctgt
     8401 cttccaggta cgtcacgccc caggtcttcg cgttcagctt cgacagcgct gcgttctgcc
     8461 ggtcgatttc ggccagattt gacgctatca agctcccctg ataccacaac accccgctca
     8521 ggctggcgaa cagcagtcct gacaccatcg tgatggtcag ccatgtccgc ccgaccatac
     8581 gcatcacgct caggcggttc gactgcatgg ccgcgctcat accctgctcg tgggcgcgga
     8641 tggcggcgct gattctcttc ccgctttcgc tcagttccgc tctgacagac ttctcgtgct
     8701 cgctgaatgc ggctttcagc atttcgccgg tactctgctg ctgcgctttc gatttctgct
     8761 ctaagtcctt cgccagcgtt aaaagactgt tcatagatgg ctcccttaag tcggatgttt
     8821 cgccctccgt ccgggtcggc aatgctgatg ctgctctttg tggtgcgcac gacctcaaaa
     8881 cctgcgcttt ccagcgcctc agtgacgtcc tgacgcgttt taagctcccc tgacgaggcg
     8941 agggccagta agccccgcgt aatagcctct gcggcttcct gcttcgtttt gggtaacgaa
     9001 gatggcgtca ccagcgcgcg ccggttctcc ggtgcgttcg ggtcgtgcag ccccagtcgc
     9061 ccgttcacca cggtctgcca cgcatcgatg cgcggacggt ctgcccggtc gtagtacggc
     9121 tggagacgcc tgccggtcag cagttccgtg ttcggtacca gaaaattcag ttccagccgc
     9181 cccttgtccc ggtgttcaac ccacagcacg ctgtactggt ctttatcgag tccgggcatc
     9241 agaacccgct cgaagctcgc catcagcttt tcacgctggc cgggcggtaa atcctgttca
     9301 gcaaaagaca ggacgccgga ggtgtacttt ttggcgtagg gcgaggcgtc gataagctcc
     9361 cggacctcct ccggctttcc ctgcaggacg atggccccgt cgcgctggcg gtctttcccc
     9421 agcagataat ccaccggccc ggcaccgccg cctcgccctc gcggatgaaa cttaacgatc
     9481 atcgtctgcc cccttttcca gcacggcatg ccgcagccgc tcgagttcgg catcaatggc
     9541 catcagcgcg gccaccacct gaacccggtc agcgcctgac cactggccgc cgttaatttt
     9601 ccgggcgatc tggttgaggt tgttccccat cccggcaagc tggcgcagca aaaccggatc
     9661 gatggacggc agccgcaacg accgcgccgg gcgcgtatcg agacaggttt cccgcatcca
     9721 caccgccagt tgccttccat tgcagcgctc cagcagctgc tggtactccc catcggtcac
     9781 ccggatacct aaaaaacggg tacgcttaac cgccatcctc tcctccgata tccgcaaaat
     9841 gtgaccgggc tgaacgctca agctctgcca tctgctcccg gcggtaatct tcctcctgtg
     9901 cctgacgcat cagatttgcc atcactgccg gatgccagga tctttttttt acgacgagcg
     9961 gcttcttctt cggcttctgc attatccctc cttcgcaaac gccggacggc acggaaaagt
    10021 tttccgggtc gctccggcgt tggacaagcg ccagcgcgtc agtggtgtta gcgggtttcg
    10081 gggcgcagcc ctgaaccagt cacagcgttt acgctgtgta tactggctta ctatgttggc
    10141 atgagagcac gattcatgaa agtgcctcat gtggcaggtg tgatatgtgg ctcaggagcg
    10201 tccgcaatac catgcacgga cagatgaaat gacgcctcct cgctcactcg gtagctgcgc
    10261 tcctgccgtt cggctgcggc gggcgtgacc gactcacgga taacgggata aagcaaaggc
    10321 gctcaaatca gaaagggccg gagaaaagga tgcggcgttg ccgtttttcc ataggctccg
    10381 cctcccctga cgagcatcac agaaatggac gctcaagtca gaggaggacg aaacccgaca
    10441 ggacttaaag atgccggtga tttctctccg gcggctccct cgtgcgctct cctgttccga
    10501 ccctgccgtt taccggatac ctgttccgcc tttttccctt tcgggaagcg tggcgctttc
    10561 tcatagctca cgctgctggt atctcggctc ggtttaggtc gttcgctcca agctgggctg
    10621 tatgcacgaa ccccccgttc agcccgactg ctgcgccctt tccggtaact atcatcttga
    10681 gtccaacccg gacggacacg caaaaacgcc actggcagca gccattggta actggatata
    10741 tacgagagag atattgagag gtcttgaagt gggtgcctaa ctgcggctac actgaaagaa
    10801 cagttttggt atctgcggtc tcgtataccc agttacccgg ttaagcagtt ccccaactga
    10861 cttaaccttc gatcaaaccg cctccccagg cggttttttc gtttacagtg caagagatta
    10921 cgcgcagaaa aaaaggatct caagaagatc ctttaaatga tcgtcagttc tgttatcccc
    10981 ggagctctat ccatcccacg gaaaaacacc cgccactgat gccctcagcg cactgcacat
    11041 catgtacaaa ccactccctc cctccttttt tattgcgttt cttaatgcgc aatggtagct
    11101 ttgtgcgcaa caaaaaacgc aatagcaagg gggggta
//
