LOCUS       NZ_CP140624             6987 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain ST152 plasmid pSO87_sm006, complete sequence.
ACCESSION   NZ_CP140624
VERSION     NZ_CP140624.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32329321
            Assembly: GCF_034554795.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Gaibani,P. and Amadesi,S.
  TITLE     Complete genome assembly and description of a Shigella sonnei
            clinical strain resistant to third- and fourth-generation
            cephalosporins isolated in Northern Italy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6987)
  AUTHORS   Gaibani,P. and Amadesi,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Microbiology, Policlinico
            Sant'Orsola-Malpighi, Via Massarenti 9, Bologna, BO 40138, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-APR-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: SO87
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 261.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 22:59:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,368
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 4,512
            CDSs (with protein)               :: 4,512
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 733
            CDSs (without protein)            :: 733
            Pseudo Genes (ambiguous residues) :: 0 of 733
            Pseudo Genes (frameshifted)       :: 204 of 733
            Pseudo Genes (incomplete)         :: 529 of 733
            Pseudo Genes (internal stop)      :: 125 of 733
            Pseudo Genes (multiple problems)  :: 119 of 733
            Pseudo Genes (short protein)      :: 3 of 733
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140624.1.
            Bacteria and source DNA available from Policlinico
            Sant'Orsola-Malpighi, Via Massarenti 9, 40138, Bologna (Italy).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="ST152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pSO87_sm006"
                     /geo_loc_name="Italy: Bologna"
                     /lat_lon="44.4912 N 11.3615 E"
                     /collection_date="2021-11-30"
                     /collected_by="IRCCS Policlinico S. Orsola - Malpighi"
     gene            join(6899..6987,1..145)
                     /locus_tag="O5Y90_RS26565"
                     /old_locus_tag="O5Y90_26560"
     CDS             join(6899..6987,1..145)
                     /locus_tag="O5Y90_RS26565"
                     /old_locus_tag="O5Y90_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137427.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLAGQVDHLSRLFITNSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(629..1051)
                     /locus_tag="O5Y90_RS26570"
                     /old_locus_tag="O5Y90_26565"
     CDS             complement(629..1051)
                     /locus_tag="O5Y90_RS26570"
                     /old_locus_tag="O5Y90_26565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001255518.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGFD
                     PNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRSTTA
                     FAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            1173..1451
                     /locus_tag="O5Y90_RS26575"
                     /old_locus_tag="O5Y90_26570"
     CDS             1173..1451
                     /locus_tag="O5Y90_RS26575"
                     /old_locus_tag="O5Y90_26570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021565938.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNARQ
                     KIFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
                     /name="hypothetical protein"
     gene            complement(1443..1733)
                     /locus_tag="O5Y90_RS26580"
                     /old_locus_tag="O5Y90_26575"
     CDS             complement(1443..1733)
                     /locus_tag="O5Y90_RS26580"
                     /old_locus_tag="O5Y90_26575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047032.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLAD
                     QRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
                     /name="hypothetical protein"
     gene            1973..3619
                     /gene="cka"
                     /locus_tag="O5Y90_RS26585"
                     /old_locus_tag="O5Y90_26580"
     CDS             1973..3619
                     /gene="cka"
                     /locus_tag="O5Y90_RS26585"
                     /old_locus_tag="O5Y90_26580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="WP_162542215.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGHG
                     NNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNPFG
                     GVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINEKQK
                     QKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKLNSYK
                     NTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVAEAEKRLAEVKAELAKAESD
                     VQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAVENELKN
                     AVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQD
                     LAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDELSKNLTKIS
                     KSLKITSRVSFLYDVGSDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVP
                     LGFWGIAIVTGIVSSYIGDDELSKLNELLGI"
                     /name="cka"
     gene            complement(3616..3906)
                     /gene="cki"
                     /locus_tag="O5Y90_RS26590"
                     /old_locus_tag="O5Y90_26585"
     CDS             complement(3616..3906)
                     /gene="cki"
                     /locus_tag="O5Y90_RS26590"
                     /old_locus_tag="O5Y90_26585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="WP_011264161.1"
                     /translation="MHLKYYLHNLPESLIPWILILIFNDNDNTPLLFIFISSIHVLLYP
                     YSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
                     /name="cki"
     gene            3936..4067
                     /locus_tag="O5Y90_RS26595"
                     /old_locus_tag="O5Y90_26590"
     CDS             3936..4067
                     /locus_tag="O5Y90_RS26595"
                     /old_locus_tag="O5Y90_26590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_077521118.1"
                     /translation="MKIIIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4262..4405)
                     /locus_tag="O5Y90_RS26600"
                     /old_locus_tag="O5Y90_26595"
     CDS             complement(4262..4405)
                     /locus_tag="O5Y90_RS26600"
                     /old_locus_tag="O5Y90_26595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_152896351.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDTQ
                     NS"
                     /name="replication initiation protein"
     gene            complement(5072..5263)
                     /locus_tag="O5Y90_RS26605"
                     /old_locus_tag="O5Y90_26600"
     CDS             complement(5072..5263)
                     /locus_tag="O5Y90_RS26605"
                     /old_locus_tag="O5Y90_26600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165980.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5331..5654
                     /locus_tag="O5Y90_RS26610"
                     /old_locus_tag="O5Y90_26605"
     CDS             5331..5654
                     /locus_tag="O5Y90_RS26610"
                     /old_locus_tag="O5Y90_26605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001444404.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPTL
                     APPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQGER
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5863..>6297
                     /locus_tag="O5Y90_RS26615"
                     /old_locus_tag="O5Y90_26610"
                     /pseudo=""
     CDS             5863..>6297
                     /locus_tag="O5Y90_RS26615"
                     /old_locus_tag="O5Y90_26610"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            6332..6892
                     /locus_tag="O5Y90_RS26620"
                     /old_locus_tag="O5Y90_26615"
     CDS             6332..6892
                     /locus_tag="O5Y90_RS26620"
                     /old_locus_tag="O5Y90_26615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_058877072.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENMLNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYLEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="MbeB family mobilization protein"
     Typing          4901..4986
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="8.73e-38"
     Typing          complement(4334..4889)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.057"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5645..6987
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.777"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cacggatttg cagcatatgt tcgagctgac acgacaggag aacgggcaac tgagggaaca
       61 gtgcgacgac ttgagtcagc aggtgcagag attagccggg caggtggacc acttgagtcg
      121 gttatttatc acgaacagca gataagggat gaacaggaaa ggcaggcacg cctggaacgg
      181 gagcgtgcgc ggtcctggca tgaagagcag gaacgggaag cacgggtatg gcgtggcccg
      241 tcaataggga tgtgacggtg agttgttccg ggaggttgcc agatggtacc ggaacgcgtt
      301 aaaaacgcat ccgggcgcga ttctggcgtg tcgtttttgt tgctgtgttt tgcctctttg
      361 ccagatcgcg ctcattcctc agcctcatcc cgcgatgtct gcgtatgtca tcaactgaaa
      421 agatggaaaa aaaacagtgt catgaggttt acgcaggcaa aaccagcgtt attcacatgg
      481 ctgaatagct atgcatacgg ttaaaattta tcaggtgcga tcgcggcagc ttttcgggtg
      541 gtttgttgcc atttttacct gtcagcagcc gtgatcgcgc tgaacgcatt tcagcggtgc
      601 gtacaattaa gggattatgg taaatccctt actgtctgcc ctcgtatcca tcgagataaa
      661 ccgcagaaat ggtgtctgcc attagtttca tttttttcgg cgaaggcata tcgctgtttg
      721 tgtattttct gccagcaaag gctgttgtgc tccggagtga gtgaaaaaat ttttctgagt
      781 ggaattcttt tacacgttgc tgtgccgttt ctgctgatac tccggctgct ttgtctttca
      841 tccccatatc ccggaactgc tgaaagaacg ggagcatcat acgtatggtg tctgctttct
      901 gggtgtagaa gttaggatca aatccatcat ctgaagcata aacataatgc cgggtatgtc
      961 gttccaggct ggtctttggt gtgcatcctg taagagctat tgttgctgtt atcagaggta
     1021 tcagaagcag cctggcagcg gcagatctca taaacatctc cttgcgtttt taatcttgat
     1081 actcagggag ttatatctcc gaagagtatc tcttatcata cgtcggttta ctcccaatgt
     1141 gatttgatgg tcaatgagtc aggaataatc ccgtgaaaaa atgtgtgcca gtcctgcttt
     1201 ttagtgttct ttctgcttcg gttgccagtc atgcggacag caatgttttt caacaaaagg
     1261 ccgagaagac aaaaaacggc attgttgcgg atgttaatgc cagacagaaa atattcagta
     1321 acagactgaa gatacaactt cagatcgatg agttgagagc agagctggct ggcaccagag
     1381 atgcccaaaa aagagaggat ttgcgtaaaa aactggagtc tctggagcgg caaaagaaat
     1441 ccttacgctg atgggggcgt attttccccg gtttcttcca taaccttttt accgttcaga
     1501 tatttacggg cgaggcgaat gcgtcttgcc tggtataccg ccgagtaagc ctgggtggga
     1561 ttcacaccat tactgatttc actgaccaca tgacgctgat cagccagtgt cagcgtgtaa
     1621 acctgtttct ggatgaggtc acgggctttg tcatgctcat atccggcagc aacaagggtg
     1681 tcgagaagtc gtttgtctgt tttgttcagt ttatggcggg gtgatttgct cacatcgttc
     1741 tccgtttttt catgatccaa cagttttata tctcatcctg atttgctccg gtgttctgtt
     1801 tcgacgttct caatgcctgc ctccccatag tgtgatcctt attgcaaaaa tgaaaatcca
     1861 tgctcttgac atggacaata ctgagtagta ggttttactg tacataaaac cagtggttat
     1921 atgtacagta tattgttttt aatttattgt tttaattatc aaagaggaat ttatggctaa
     1981 agaactaagt ggatatggac caactgctgg tgagtctatg gggggaacag gggcgaatct
     2041 gaatcagcaa ggtggaaata ataatagtaa tagtggggtt cactggggtg gtggctctgg
     2101 gcatggtaat aatggtggtc agggaaattc aaattcatct ggttctacat caactgtgat
     2161 gaaaaccggt gagtcatatc tgactccgtg gggtgatgtt gtcattaata atgatggcct
     2221 tccggtgatg aatggtattg ttatgacaga agagaattca actctggttg ataatccatt
     2281 tggaggggtc tccagagttc ttaattcttt aattagtgat atgccatcac tttttgcaga
     2341 aagctcagga aataacaaca ataacactgc ttcagttaat actgcaccaa cgaatgctca
     2401 ggtcagtgat atggataaga gttcgaaggt ggtgggtaat gtaattaatg aaaaacaaaa
     2461 gcagaagaat aaaattgcta cacagattag tgaaaaacag aagaaaatcg aggagatgaa
     2521 aaaagtattc aagcatcatt cttatcatgg tatcactgat cttgagaggg atgttgatga
     2581 gttgcaaaaa aaatcaaatc agcttgatgc agatattagc aaacttaatt cttataaaaa
     2641 tacattgcaa agtaaaattg gtgatgtgaa taaacagaag gaagctgaag aaaaggctcg
     2701 tgaaaatgca gaggtcgctg aacatgaaac tcttaatgaa gagaaacagg cggtggcgga
     2761 agcagagaag cgtttagctg aggtgaaagc tgaactggcg aaggctgaaa gtgatgtaca
     2821 gagtaagcaa gctactgttt ccagagttgc aggagagctt gaaaacgctc agaagagtgt
     2881 tgacgtgaag gtcaccggat ttccgggatg gcgtgacgtt cagaaaaaac tacagcgtca
     2941 gcttgaagct aagcaggctg agtactccgc agttgaaaat gaacttaaga atgctgttag
     3001 tttcagggat ggtaaggccg ctgaggttaa agaggcagag cagaaattaa aagaagctca
     3061 ggatgctctt gaaaagagcc agataaaaga tgctgttgat actatggttg gattttatca
     3121 atatataacc gaacaatatg gagaaaaata cgcaaaaatt gctcaggatt tagcggaaaa
     3181 gtctaaagga aagaaaattc aaggtgttga tgaagctctg gcagcatttg aaaagtataa
     3241 gaatgtactg gataagaaat tcagtaaggt tgatcgggat gctattttta atgcgttaga
     3301 gtctgttaat tatgatgagt tatctaagaa tttaacgaag atatcaaagt cgcttaaaat
     3361 caccagtcga gtatcttttc tatatgatgt tggttctgat tttaaaaatg caatagaaac
     3421 tggtaactgg cgacctttgt ttgtcacgct ggagaaaagt gcggtcgatg taggtgtggc
     3481 aaaaattgtg gcattaatgt ttagttttat tgttggtgtg cctcttggtt tctgggggat
     3541 tgcaattgtc acaggtattg tttcttctta cataggggat gatgagttga gcaaacttaa
     3601 tgagttgtta gggatttaat ttctctttag agtgtaatat atgaaacttg gcaatcctac
     3661 tgggattgcc attaataaat aaaacaatgc agataacttg cataagtacc agggttcttt
     3721 ttttaacttt gtattttctt tgatatatct agatatggtt aatttagagt atggatatag
     3781 caatacatgt attgatgata taaatataaa taacaaagga gtgttatcat tgtcgttaaa
     3841 tattaaaata agaatccatg gtataagtga ttcaggtaaa ttatgtaggt agtattttaa
     3901 atgcataagt atttttactt ttgttggaaa gaaatatgaa aataataatt tttctaatta
     3961 caatattatt cttgtcagga tgtcaagcaa actatattcg tgatgtacaa ggaggaaccg
     4021 ttgcaccatc ttcgtcttct gaactgacgg ggatcgcggt tcagtagaaa agatcaaagg
     4081 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ctgtaaacga aaaaaccgcc
     4141 tggggaggcg gtttagtcga aggttaagtc agttggggac tgcttaaccg ggtaactggc
     4201 tttagtggag cacagatacc aaatgctgtc cttccagtgt agccgctata aggcttcaac
     4261 ttcaagagtt ctgcgtatct aaatccacta tccagttacc aatggctgct gccagtggcg
     4321 ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     4381 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4441 tgagatacca acagcgtgag ctatgagaaa gcgccacgct tcccaaaggg agaaaggcgg
     4501 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag ccaccagggg
     4561 gaaacgcctg gtatctttaa gtcctgtcgg gtttcgccac ctctgacttg agcgtcggtt
     4621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagctgcg tggccttttc
     4681 cgggttcttg ttttttgctc acatgtttct tttggtgtta tcccctgatt ctgtggataa
     4741 cagcatttcg tttttgagtg agtagatacc gctcgccgca gtcgaacgac cgagcgtagc
     4801 gagtcagtga gcgaggaagc ggaaaagcgg ctggacgtgc attttctcct tacgcatctg
     4861 tgcggcattt cacacccggt atggcgcact tttcatacca tccgcactga tgccgcatgg
     4921 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     4981 cgctaaaacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gctcacaggc
     5041 cagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tccccgaagc
     5101 gtgcgaggca gctgcggtaa agctcgtcag cgtggtcgtg aagcgcttcg cagatgtcag
     5161 cctggtcgtc ggcgtccagt tcattcagtt tttccagcag ggtcagcgtc tggcttctga
     5221 tgaagcgggc catgttaagg gcggtttttt cctgtttagt cacagttctg tttctccatg
     5281 tgtggggagt ctgtcggagg tttttaatga taccggtgaa acgcgaaagg atgctcacga
     5341 tacgggtcac ggatgaggaa catgcgcggc tgctggcacg ttgtgaggga aaacaacttg
     5401 cgtcatggat gcgtaaggtc tgcctggggg caccgccatc gaaaacgtca ggtcttccga
     5461 cactggcacc gccgctgttg cgccagttcg cttcggtcgg caataacctg aatcagatag
     5521 cccggaagat aaacagcgga cagtggtcgg ggcatgaccg ggttcatgtg gtggctgcac
     5581 tgatggccat cggacgtgaa ctgagtgagc tgagggatga ggtccgtaaa cagggagaac
     5641 gggatgatag ttaagtttca tgccagggga aaaggcggtg gcagtggtcc ggttgattac
     5701 ctgctgggcc gggagcgtaa ccgggaaggg gcgagagttc tgcggggtgc tcccgaagag
     5761 gtgcgggaac tcatcgatgc cacgccgttt gcgaaaaaat acacctcggg ggttctgtct
     5821 tttgcagagc agaccctgcc accgggagag cgtgaaaggg tgatggagag ctttgagcgg
     5881 gtactgatgc ccggtcttga aaaaaatcag tacagcatcc tgtgggtgga gcaccaggac
     5941 aaggggcgtc ttgagctgaa ttttgtcatc ccgaatatgg agctggcgag cggaaaacgg
     6001 ctccagccct attacgaccg agcggaccgt ccgcgaatca atgcctggca gacactggtg
     6061 aatcaccatt acgggctgca cgacccgaac gcaccggaga acaggcggac acttgtcacc
     6121 ccgaataatc tgccgaaagc gaaacaggag gccgcagaag cgattacgag gggactggag
     6181 gctctttacc atgccggagc gctaaaaacg cgtcaggatg tcactgaggc gcttacagcg
     6241 gcaggttttg aggttgtgag gacaaccagg agcagtatca gtatcgccga cccggagggt
     6301 gggcgtaatc tcaggctgaa aggggcgctg tatgagcaat cttttacaaa tggggacaga
     6361 cttcgagaag aaactgaaag agcgtgccgt ctctacagag aacatgctga acagcgagtt
     6421 caggaagctc gaagcgtctg tagacaggga actgaactta aacgggcaga aaatcagaga
     6481 cgctatcagc gcacacacag cagcggtgaa agaacaactg gagacgctga acagtgccgt
     6541 gaacgcgcag ttcagcgcca cagagaacga actggcccgg cggcaggaag agctgctctg
     6601 gaagctggtc aaagggcgga tactgtaccc gtcgctgacg gcgctttgcg tcacgggcgg
     6661 gatttttctg gcgtcatggg ggctgattca gtggcaggaa agccggattg cggcgaacat
     6721 cctggctatc agggaccagg aagagacgct ggcgaaactc cgggaaaaaa catggggtgt
     6781 gacgtatctc gagggcagga acgggaaatt tctggtgttg ccgtccgggg tgaaggggga
     6841 aaacaactgg acggtggaga agaagaacgc agtaagactg gtcagggagt aagtgagtat
     6901 gacagagctg gaaatgcact tactgaacgc cttagagcaa ttgcagcagg actatacgca
     6961 gcggctgaaa gactgggaga gcgcctt
//
