LOCUS       NZ_CP140623             8390 bp    DNA     circular CON 07-JAN-2024
DEFINITION  Shigella sonnei strain ST152 plasmid pSO87_sm005, complete sequence.
ACCESSION   NZ_CP140623
VERSION     NZ_CP140623.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN32329321
            Assembly: GCF_034554795.1
KEYWORDS    RefSeq.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Gaibani,P. and Amadesi,S.
  TITLE     Complete genome assembly and description of a Shigella sonnei
            clinical strain resistant to third- and fourth-generation
            cephalosporins isolated in Northern Italy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   Gaibani,P. and Amadesi,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-DEC-2022) Microbiology, Policlinico
            Sant'Orsola-Malpighi, Via Massarenti 9, Bologna, BO 40138, Italy
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 13-APR-2022
            Assembly Method                   :: Unicycler v. 0.5.0
            Assembly Name                     :: SO87
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 261.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 22:59:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,368
            CDSs (total)                      :: 5,245
            Genes (coding)                    :: 4,512
            CDSs (with protein)               :: 4,512
            Genes (RNA)                       :: 123
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 733
            CDSs (without protein)            :: 733
            Pseudo Genes (ambiguous residues) :: 0 of 733
            Pseudo Genes (frameshifted)       :: 204 of 733
            Pseudo Genes (incomplete)         :: 529 of 733
            Pseudo Genes (internal stop)      :: 125 of 733
            Pseudo Genes (multiple problems)  :: 119 of 733
            Pseudo Genes (short protein)      :: 3 of 733
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140623.1.
            Bacteria and source DNA available from Policlinico
            Sant'Orsola-Malpighi, Via Massarenti 9, 40138, Bologna (Italy).
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8390
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /strain="ST152"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /plasmid="pSO87_sm005"
                     /geo_loc_name="Italy: Bologna"
                     /lat_lon="44.4912 N 11.3615 E"
                     /collection_date="2021-11-30"
                     /collected_by="IRCCS Policlinico S. Orsola - Malpighi"
     gene            complement(224..373)
                     /locus_tag="O5Y90_RS26510"
                     /old_locus_tag="O5Y90_26505"
     CDS             complement(224..373)
                     /locus_tag="O5Y90_RS26510"
                     /old_locus_tag="O5Y90_26505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001138880.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            944..1102
                     /locus_tag="O5Y90_RS26515"
                     /old_locus_tag="O5Y90_26510"
     CDS             944..1102
                     /locus_tag="O5Y90_RS26515"
                     /old_locus_tag="O5Y90_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032287700.1"
                     /translation="MHEPPVQSDRCALSGNYQLESNPERHDKTPLAAAISNRSAREDRL
                     RGLEVEA"
                     /name="hypothetical protein"
     gene            1562..2377
                     /gene="sul2"
                     /locus_tag="O5Y90_RS26520"
                     /old_locus_tag="O5Y90_26515"
     ARG             1563..2374
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            2438..3241
                     /gene="aph(3'')-Ib"
                     /locus_tag="O5Y90_RS26525"
                     /old_locus_tag="O5Y90_26520"
     ARG             2439..3238
                     /name="aph(3'')-Ib"
                     /gene="aph(3'')-Ib"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3'')-Ib"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="99.63"
                     /coverage="100.0"
     gene            3241..4077
                     /locus_tag="O5Y90_RS26530"
                     /old_locus_tag="O5Y90_26525"
     CDS             3241..4077
                     /locus_tag="O5Y90_RS26530"
                     /old_locus_tag="O5Y90_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="WP_000480968.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLKP
                     IEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATEIA
                     AELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMMSNA
                     SELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDPRRIA
                     QMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQTSY"
                     /name="aminoglycoside O-phosphotransferase APH(6)-Id"
     gene            4383..4625
                     /locus_tag="O5Y90_RS26535"
                     /old_locus_tag="O5Y90_26530"
     CDS             4383..4625
                     /locus_tag="O5Y90_RS26535"
                     /old_locus_tag="O5Y90_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="WP_000844627.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERGR
                     EAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
                     /name="transposase"
     gene            complement(4657..5307)
                     /gene="tetR(A)"
                     /locus_tag="O5Y90_RS26540"
                     /old_locus_tag="O5Y90_26535"
     CDS             complement(4657..5307)
                     /gene="tetR(A)"
                     /locus_tag="O5Y90_RS26540"
                     /old_locus_tag="O5Y90_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional repressor
                     TetR(A)"
                     /protein_id="WP_000164043.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
                     /name="tetR(A)"
     gene            5413..6612
                     /gene="tet(A)"
                     /locus_tag="O5Y90_RS26545"
                     /old_locus_tag="O5Y90_26540"
     ARG             5414..6609
                     /name="tet(A)"
                     /gene="tet(A)"
                     /gene_name="tetracycline efflux MFS transporter Tet(A)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(<6579..6767)
                     /locus_tag="O5Y90_RS26550"
                     /old_locus_tag="O5Y90_26545"
                     /pseudo=""
     CDS             complement(<6579..6767)
                     /locus_tag="O5Y90_RS26550"
                     /old_locus_tag="O5Y90_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /name="IS91 family transposase"
     gene            complement(6811..7200)
                     /locus_tag="O5Y90_RS26555"
                     /old_locus_tag="O5Y90_26550"
     CDS             complement(6811..7200)
                     /locus_tag="O5Y90_RS26555"
                     /old_locus_tag="O5Y90_26550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000102614.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            7418..7858
                     /locus_tag="O5Y90_RS26560"
                     /old_locus_tag="O5Y90_26555"
     CDS             7418..7858
                     /locus_tag="O5Y90_RS26560"
                     /old_locus_tag="O5Y90_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_005119689.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     ARG             3242..4074
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          complement(409..582)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          506..1111
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcgtaaatca cccgccatgg ttgattattt ttcaacctca aaatggtcat acacctaacg
       61 cagccgcgat tttcatcgca actgcatgaa ttaaaagaaa aaaaaggaat atttcgtttt
      121 cacgagtaat acccgggtat taaaaatcca ctgtaattac atgaaaataa agaaataaaa
      181 ggtaataccg gagtattacc acgtacgcgg gcaagcgaaa aagctataac gcttcccggc
      241 cgacaggcga cccaaaatcc acaggggcat gcaggttctc ttcagtaacc tgcgccatta
      301 aatcaaaaag gtcatattcg ccgggaagct gcgtatcagt cgaatccagt tgttcatcat
      361 attcagatgg catggggtga tatccagtag ctgagaggtt gttagcgggt ttcggggcgc
      421 agccctgaac cagtcatgta gcgctagcgg agtgtatact ggcttactat gttgccacga
      481 ttgcgaaaat cagtgaagtg cttcatgtgg caggagaaaa aaggcagcac cggcgcgtca
      541 gcagaatatg cgatacagga gatattccgc ttcctcgctc actgactcgc tacgctcggt
      601 cgttcggctg cggcgagcgg taccggctta cggacgggga cggaaatttc ctggaagata
      661 ccaggaagac acttagcaaa gaagagataa ggccacggcg aagccgtttt tccataggct
      721 ccgcccccct gacaagcatc gcaaaatctg acgctcaaat cagtggtggc gaaacccgac
      781 aggactataa agatcccagg cgtttccccc tggtagctcc ctcgtgcgct ctcctgttcc
      841 tgcctttcgg tttaccggtg tcattccgct gttatggccg cggtttctca ttccacgcct
      901 gacactcagt tccgtgaagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
      961 tcagtccgac cgctgcgcct tgtccggtaa ctatcaactt gagtccaacc cggaaagaca
     1021 cgacaaaacg ccactggcag cagccattag taacaggagt gcacgagaag atagattaag
     1081 aggtcttgaa gtagaggcct gactgcggct acactgaaag aacagttttg gtaatggcgg
     1141 tctcgtgaac cctgttaccg ggttaagcag ttccccaact gacttaacct tcgaacaaac
     1201 cgcctcccca ggcggttttt tcgtttacag agcaagagat tacgattcat tgaaaaggat
     1261 ctcaagaaga tcctttgtag attactatca attaatcttc cgaacatgac accagtcact
     1321 gttgattacg gttaaaccgc tccgtaacag gtttccctcg tattggtgac ttgcagcacc
     1381 aaagaagcaa aaaagataaa tatatctact tattgagaga tgatatattt atttccaaaa
     1441 tgctacagat acatcatcat gaaacggatc accgcaaaca ggttactcgc ctggggattc
     1501 cctttcgacc cgagcatccg tatgagactc atgctcgatt attattatta tagaagcccc
     1561 catgaataaa tcgctcatca ttttcggcat cgtcaacata acctcggaca gtttctccga
     1621 tggaggccgg tatctggcgc cagacgcagc cattgcgcag gcgcgtaagc tgatggccga
     1681 gggggcagat gtgatcgacc tcggtccggc atccagcaat cccgacgccg cgcctgtttc
     1741 gtccgacaca gaaatcgcgc gtatcgcgcc ggtgctggac gcgctcaagg cagatggcat
     1801 tcccgtctcg ctcgacagtt atcaacccgc gacgcaagcc tatgccttgt cgcgtggtgt
     1861 ggcctatctc aatgatattc gcggttttcc agacgctgcg ttctatccgc aattggcgaa
     1921 atcatctgcc aaactcgtcg ttatgcattc ggtgcaagac gggcaggcag atcggcgcga
     1981 ggcacccgct ggcgacatca tggatcacat tgcggcgttc tttgacgcgc gcatcgcggc
     2041 gctgacgggt gccggtatca aacgcaaccg ccttgtcctt gatcccggca tggggttttt
     2101 tctgggggct gctcccgaaa cctcgctctc ggtgctggcg cggttcgatg aattgcggct
     2161 gcgcttcgat ttgccggtgc ttctgtctgt ttcgcgcaaa tcctttctgc gcgcgctcac
     2221 aggccgtggt ccgggggatg tcggggccgc gacactcgct gcagagcttg ccgccgccgc
     2281 aggtggagct gacttcatcc gcacacacga gccgcgcccc ttgcgcgacg ggctggcggt
     2341 attggcggcg ctgaaagaaa ccgcaagaat tcgttaactg cacattcggg atatttctct
     2401 atattcgcgc ttcatcagaa aactgaagga acctccattg aatcgaacta atattttttt
     2461 tggtgaatcg cattctgact ggttgcctgt cagaggcgga gaatctggtg attttgtttt
     2521 tcgacgtggt gacgggcatg ccttcgcgaa aatcgcacct gcttcccgcc gcggtgagct
     2581 cgctggagag cgtgaccgcc tcatttggct caaaggtcga ggtgtggctt gccccgaggt
     2641 catcaactgg caggaggaac aggagggtgc atgcttggtg ataacggcaa ttccgggagt
     2701 accggcggct gatctgtctg gagcggattt gctcaaagcg tggccgtcaa tggggcagca
     2761 acttggcgct gttcacagcc tatcggttga tcaatgtcag tttgagcgca ggctgtcgcg
     2821 aatgttcgga cgcgccgttg atgtggtgtc ccgcaatgcc gtcaatcccg acttcttacc
     2881 ggacgaggac aagagtacgc cgcagctcga tcttttggct cgtgtcgaac gagagctacc
     2941 ggtgcggctc gaccaagagc gcaccgatat ggttgtttgc catggtgatc cctgcatgcc
     3001 gaacttcatg gtggacccta aaactcttca atgcacgggt ctgatcgacc ttgggcggct
     3061 cggaacagca gatcgctatg ccgatttggc actcatgatt gctaacgccg aagagaactg
     3121 ggcagcgcca gatgaagcag agcgcgcctt cgctgtccta ttcaatgtat tggggatcga
     3181 agcccccgac cgcgaacgcc ttgccttcta tctgcgattg gaccctctga cttggggttg
     3241 atgttcatgc cgcctgtttt tcctgctcat tggcacgttt cgcaacctgt tctcattgcg
     3301 gacacctttt ccagcctcgt ttggaaagtt tcattgccag acgggactcc tgcaatcgtc
     3361 aagggattga aacctataga agacattgct gatgaactgc gcggggccga ctatctggta
     3421 tggcgcaatg ggaggggagc agtccggttg ctcggtcgtg agaacaatct gatgttgctc
     3481 gaatatgccg gggagcgaat gctctctcac atcgttgccg agcacggcga ctaccaggcg
     3541 accgaaattg cagcggaact aatggcgaag ctgtatgccg catctgagga acccctgcct
     3601 tctgcccttc tcccgatccg ggatcgcttt gcagctttgt ttcagcgggc gcgcgatgat
     3661 caaaacgcag gttgtcaaac tgactacgtc cacgcggcga ttatagccga tcaaatgatg
     3721 agcaatgcct cggaactgcg tgggctacat ggcgatctgc atcatgaaaa catcatgttc
     3781 tccagtcgcg gctggctggt gatagatccc gtcggtctgg tcggtgaagt gggctttggc
     3841 gccgccaata tgttctacga tccggctgac agagacgacc tttgtctcga tcctagacgc
     3901 attgcacaga tggcggacgc attctctcgt gcgctggacg tcgatccgcg tcgcctgctc
     3961 gaccaggcgt acgcttatgg gtgcctttcc gcagcttgga acgcggatgg agaagaggag
     4021 caacgcgatc tagctatcgc ggccgcgatc aagcaggtgc gacagacgtc atactagata
     4081 tcaagcgact tctcctatcc cctgggaaca catcaatctc accggagaat atcgctggcc
     4141 aaagccttag cgtaggattc cgccccttcc cgcaaacgac ccctggaagc cccgtagcga
     4201 cgcggagagg ggcgagacaa gccaagggcg caggctcgat gcgcagcacg acatagccgg
     4261 ttctcgcaag gacgagaatt tccctgcggt gcccctcaag tgtcaatgaa agtttccaac
     4321 gcgagccatt cgcgagagcc ttgagtccac gctagatcta tctcatctgc gcaaggcaga
     4381 acgtgaagac ggccgccctg gacctcgccc gcgagcgcca ggcgcacgag gccggcgcgc
     4441 ggacccgcgc cacggcccac gagcggacgc cgcagcagga gcgccagaag gccgccagag
     4501 aggccgagcg cggccgtgag gcttggacgc tagggcaggg catgaaaaag cccgtagcgg
     4561 gctgctacgg gcgtctgacg cggtggaaag ggggagggga tgttgtctac atggctctgc
     4621 tgtagtgagt gggttgcgct ccggcagcgg tcctgatcaa tcgtcaccct ttctcggtcc
     4681 ttcaacgttc ctgacaacga gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc
     4741 ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag
     4801 cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc
     4861 ctgctcctca agcacggccc caacagtgaa gtagctgatt gtcatcagcg cattgacggc
     4921 gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat
     4981 ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag
     5041 cgcctgcctg aagctgcggg cattcccgat cagaaatgag cgccagtcgt cgtcggctct
     5101 cggcaccgaa tgcgtatgat tctccgccag catggcttcg gccagtgcgt cgagcagcgc
     5161 ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga acccccaacc gttccgccag
     5221 tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg cggcacggat
     5281 cactgtattc ggctgcaact ttgtcatgct tgacacttta tcactgataa acataatatg
     5341 tccaccaact tatcagtgat aaagaatccg cgcgttcaat cggaccagcg gaggctggtc
     5401 cggaggccag acgtgaaacc caacagaccc ctgatcgtaa ttctgagcac tgtcgcgctc
     5461 gacgctgtcg gcatcggcct gattatgccg gtgctgccgg gcctcctgcg cgatctggtt
     5521 cactcgaacg acgtcaccgc ccactatggc attctgctgg cgctgtatgc gttgatgcaa
     5581 tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg gccggtcttg
     5641 ctcgtctcgc tggccggcgc tgctgtcgac tacgccatca tggcgacggc gcctttcctt
     5701 tgggttctct atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc
     5761 ggcgcttata ttgccgatat cactgatggc gatgagcgcg cgcggcactt cggcttcatg
     5821 agcgcctgtt tcgggttcgg gatggtcgcg ggacctgtgc tcggtgggct gatgggcggt
     5881 ttctcccccc acgctccgtt cttcgccgcg gcagccttga acggcctcaa tttcctgacg
     5941 ggctgtttcc ttttgccgga gtcgcacaaa ggcgaacgcc ggccgttacg ccgggaggct
     6001 ctcaacccgc tcgcttcgtt ccggtgggcc cggggcatga ccgtcgtcgc cgccctgatg
     6061 gcggtcttct tcatcatgca acttgtcgga caggtgccgg ccgcgctttg ggtcattttc
     6121 ggcgaggatc gctttcactg ggacgcgacc acgatcggca tttcgcttgc cgcatttggc
     6181 attctgcatt cactcgccca ggcaatgatc accggccctg tagccgcccg gctcggcgaa
     6241 aggcgggcac tcatgctcgg aatgattgcc gacggcacag gctacatcct gcttgccttc
     6301 gcgacacggg gatggatggc gttcccgatc atggtcctgc ttgcttcggg tggcatcgga
     6361 atgccggcgc tgcaagcaat gttgtccagg caggtggatg aggaacgtca ggggcagctg
     6421 caaggctcac tggcggcgct caccagcctg acctcgatcg tcggacccct cctcttcacg
     6481 gcgatctatg cggcttctat aacaacgtgg aacgggtggg catggattgc aggcgctgcc
     6541 ctctacttgc tctgcctgcc ggcgctgcgt cgcgggcttt ggagaaattc ttcaaattcc
     6601 cgttgcacat agcccggcaa ttcctttccc tgctctgcca taagcgcagc gaatgccggg
     6661 taatactcgt caacgatctg atagagaagg gtttgctcgg gtcggtggct ctggtaacga
     6721 ccagtatccc gatcccggct ggccgtcctg gccgccacat gaggcatgtt ccgcgtcctt
     6781 gcaatactgt gtttacatac agtctatcgc ttagcggaaa gttcttttac cctcagccga
     6841 aatgcctgcc gttgctagac attgccagcc agtgcccgtc actcccactc ttacattgtt
     6901 cgcagtatct aggtacatgt tattgcgtgc accttcatga tgtataatgt tattaatgac
     6961 agcagaaaga tcgacagaat taatattctt cgtacctggg acagttatcg ggtgtcggcc
     7021 agtgatgaga ccaaaggatg cagtataaga tgaaccacta ctattatcat ggccaccatt
     7081 accaccaccg ttaaaactac ctcccgaact tccagagccg ctatgaccat cattgtaggg
     7141 gtcatttggt cccatgtgac caaaaccacc gacagtaccg ctatttaact ctgtactcat
     7201 aaaattatcc tctttgactt ttaaaaacag caaaaactgt atttataaca atgtgtttat
     7261 atacagtatt cgaaatacta cgcccgtcaa aaccacaccg tcaacaggtt ttttgtgtat
     7321 ttttcacgcc tcacgggcag cctcagacct tttgtgttaa aacagtaata tgttctgctg
     7381 ttacaatacg gtacacacag gagacactgt cacttttatg gcatggcaca cagttcaaga
     7441 gacactgaaa ttaacaggaa agtcgcgctc gcaattatat cgtgacatgg ctaaaggcct
     7501 tgtcagctat cgcacaggaa aaaatgatcg ccgggagttc gagaccagtg aactgattcg
     7561 ggcctatggc gagctgcgca cttctgagac accaaaaagg cacaaggagg gacactccaa
     7621 atctgtcgtt agccaggcgg aaaatgctca gctagacgtc attcagcaga aactggacgc
     7681 tctgcagcag actgttaccc taatgcttga agacaagatg gcgcgggaag cagaaaaccg
     7741 cctgcgaaat gaggagaggg aacaactaca ggccgaaatt agccgtctgg ctgacgcgct
     7801 ggcacaggaa aaaaagcggg gattctggtc gcggctgttt cgctccggtt cagagtaaca
     7861 gtgcaggtga ctctgattta ccataacatc tgttatcagc accgcctgag aggccctcag
     7921 ggcgtttctg acgggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa
     7981 cgcgcaggct ctgtttttac ggcatgcatg agtatgcgca caagtgcata gcgcggaaat
     8041 tcacaccatt cagccggggt aagtcctgtt tccggcgttc ttcccatccg aaaacggtga
     8101 acggggacgc aatttattgc gtggccgtga agcgttgccg gcgcagccgg aaaacccgct
     8161 gtcagcacgt tcaggaaggg ggtgatgtac aaatggcaca gaggatgaaa gtgacgtatg
     8221 agctgaagga atgatgtaaa agtgatgtat aagtgatgta aaagtgatgt ataagtgatg
     8281 tatgaactga aggagcgatg taattctgta gtcaggaaga ataacccgtt catgcaggca
     8341 tgaacgatac agtgaggaac gctttttttg tcacgatctc cccttaacct
//
