LOCUS       NZ_CP140514             4167 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 2023CK-01432 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP140514
VERSION     NZ_CP140514.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38646027
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 4167)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (06-DEC-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/06/2024 05:26:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6
            CDSs (total)                      :: 6
            Genes (coding)                    :: 5
            CDSs (with protein)               :: 5
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 1 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140514.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023CK-01432"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2023-10-13"
     gene            complement(240..872)
                     /locus_tag="U4X41_RS00005"
                     /old_locus_tag="U4X41_29170"
     CDS             complement(240..872)
                     /locus_tag="U4X41_RS00005"
                     /old_locus_tag="U4X41_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="WP_012579086.1"
                     /translation="MKDINNSKKIIFVEGDDDFHFLCNLLEIQGITDVFVEKINGKSKL
                     KQALRAFKNIDSFDEKESIFIILDADTSFSDTERSIIATLRELQISSPSSHNEIAMNGN
                     TKISFFIMPGKDKQGAIEDLIIEHASTRALYEYIIEFFEKIKNNKDDISASDKEYTYPT
                     NEKKAKVQVYLSCNRESDSRIGISMKNGIINTADNCFDEIKDFISKI"
                     /name="DUF3226 domain-containing protein"
     gene            complement(872..1987)
                     /locus_tag="U4X41_RS00010"
                     /old_locus_tag="U4X41_29175"
     CDS             complement(872..1987)
                     /locus_tag="U4X41_RS00010"
                     /old_locus_tag="U4X41_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019405083.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="WP_012579087.1"
                     /translation="MIKEISLKNFKCFNELYLKQLKTLNIIAGKNNYGKTSILDAIFCF
                     YDVKNPAVLLNIQAFRKEMAEINKNKPFWVSYFHDMDTSQKMSIVIRDERSEVTQTYET
                     ETNQRLESSLSLNVDTVLSNQNIPRQTIGSQATVNSLKINVTETPNAKNRVSNKLFSMQ
                     LKEDGVEIKSEIKNERDGINYKFKTATIITTSRKINKEATITNVSSLLTQKRKKDILEN
                     LKKIDDRIVDIAISAIGNNKEIYLDIGFSELNEISMLGEGISRALSFISSVLVQENSII
                     LIDEIENGIHYSVIKDIIKSLISSAKQNNNQIFATTHSQDVIRAINEIDSKNEDIAYIR
                     LGREKNSLKPTAVQFNMDDFSYSVENDWEVR"
                     /name="ATP-binding protein"
     gene            complement(2332..2445)
                     /locus_tag="U4X41_RS00015"
                     /old_locus_tag="U4X41_29180"
                     /pseudo=""
     CDS             complement(2332..2445)
                     /locus_tag="U4X41_RS00015"
                     /old_locus_tag="U4X41_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2861..3052
                     /locus_tag="U4X41_RS00020"
                     /old_locus_tag="U4X41_29185"
     CDS             2861..3052
                     /locus_tag="U4X41_RS00020"
                     /old_locus_tag="U4X41_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3052..3453
                     /locus_tag="U4X41_RS00025"
                     /old_locus_tag="U4X41_29190"
     CDS             3052..3453
                     /locus_tag="U4X41_RS00025"
                     /old_locus_tag="U4X41_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_012579088.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSGQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3799..4041)
                     /locus_tag="U4X41_RS00030"
                     /old_locus_tag="U4X41_29195"
     CDS             complement(3799..4041)
                     /locus_tag="U4X41_RS00030"
                     /old_locus_tag="U4X41_29195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_098963735.1"
                     /translation="MHSAEIHTIQPGEVLFPAFFSSENGERGRNLLRGREALPAQPENR
                     FSAPSGIGFVDGRQSRYGKSPDDSPHDSPAIRIVN"
                     /name="hypothetical protein"
     Typing          complement(2333..2740)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.399"
                     /coverage="99.0"
                     /evalue="5.92e-171"
ORIGIN
        1 acaattatga ttttatgtta aattgtttta agtgaataga gagaatataa cttaagggag
       61 catcttgtgc tgggtctttt acattagatc tgcacaatca ttgttttggc atctggtgat
      121 gtctttagga cgtttgagat tgctgataac taaagtgtat gatgctttct atacgttatc
      181 cggcgtttaa taacaccctg gtgataagcc agcccgtgaa gggctggctt gatttggatt
      241 tatatttttg aaataaaatc ttttatttca tcaaagcaat tgtctgctgt gtttattatt
      301 ccatttttca tggatattcc tattcttgag tcggattctc tattgcagct aagatagact
      361 tgtaccttag cttttttttc gtttgttgga taagtgtact ctttgtcact cgctgagatg
      421 tcatctttat tatttttaat tttttcgaaa aattctatta tgtattcata taacgctcga
      481 gtgcttgcgt gttcaattat cagatcttca atggcacctt gcttgtcttt tcctggcatt
      541 ataaagaaag aaatttttgt attgccattc atagctatct cgttatgcga gctgggggag
      601 gagatttgta gttctcgcaa tgtggcaatt attgatcttt ctgtgtcaga aaatgatgtg
      661 tcagcatcta atataataaa aatactttct ttttcatcga aagaatcaat gtttttgaat
      721 gctctaagtg cttgttttaa ttttgatttt ccatttattt tttcgacaaa aacatcggta
      781 atgccttgta tttcaagtag gttgcataga aaatgaaaat catcatcacc ctcaacgaaa
      841 atgatctttt tgctattatt tatgtctttc attatcttac ctcccagtcg ttctcaactg
      901 agtaggagaa atcatccata ttgaattgta cggctgttgg ttttagggag ttcttttctc
      961 ttcctagtct aatataagcg atatcttcat ttttgctatc tatttcgttt attgctcgta
     1021 tcacatcttg actatgagtt gttgcaaata tttgattgtt gttttgtttt gcagatgaaa
     1081 ttaatgattt tattatgtct ttaattactg agtaatgtat tccgttctcg atttcatcaa
     1141 taagaataat tgagttttcc tgtaccagca ctgagctgat aaaagataat gctcgactga
     1201 taccctcgcc aagcattgat atttcattta gctcactaaa tccaatgtct aaatatattt
     1261 ccttattgtt acctatcgca gatattgcta tatctacaat tctgtcatct atttttttta
     1321 agttttctag tatgtctttt ttcctttttt gtgttagtag gctagataca tttgtaattg
     1381 ttgcctcttt gtttatcttt ctggatgttg ttattattgt tgctgtctta aatttataat
     1441 taatcccatc cctttcgttt tttatttcgg attttatttc tacgccatct tctttcaatt
     1501 gcatagagaa aagcttattg gaaaccctgt ttttcgcatt gggtgtctcg gtcacattta
     1561 tttttaaaga gtttactgtg gcctgagaac cgatagtttg acgaggtatg ttttggttag
     1621 atagtacggt gtcaacgttt agagacaaac ttgattctaa tctctgattt gtttctgttt
     1681 catatgtttg tgttacttca gagcgttcat ctcgaatgac tatggacatc ttttgtgatg
     1741 tgtccatatc atgaaagtaa ctaacccaaa atggcttgtt tttgtttatt tctgccattt
     1801 cttttctgaa tgcttgaatg tttaacaaaa cagccgggtt ttttacatca taaaaacaaa
     1861 aaatagcatc cagtatagat gttttcccgt agttgttttt cccagctatg atatttaaag
     1921 tctttaattg ctttagatat agctcattga agcatttgaa gttctttaaa cttatttcct
     1981 ttatcatgat agcctctgtt cgtgtttttg ttatatgcca tacgatttgt tagtacctta
     2041 ataaaatgta cagctttcat tgtgattagc tagactttat gtcaaagatt aactttgatt
     2101 gcttgtgttt taatcgattt tttttacttt cttcttgaga ttatctggca gcagccgtaa
     2161 gcttccggct gtgaacgaaa aaaaccgcct tggcgggcgg ttttttcgaa aggttcagta
     2221 gagttggcgc tctatgaacc gaggtaactg gcttaaaggg agcgcagtca ccaaaactgt
     2281 tcgtttagtt tagctttaac tggcgtataa cttcaagact aactccctaa atcagttacc
     2341 agtggctgct gccagtggcg ctttgtcgtg tctttccggg ttggactcaa gacgatagtt
     2401 accggattag gcgcagcagt cggactgaac ggggggttcg tgcatacagt ccagcttgga
     2461 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca
     2521 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccatcag
     2581 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
     2641 agattccgtg atgctcgtca ggggggcgga gcctatggaa aaacggccgc tgtgcggcct
     2701 ttccttcttt cctccatgcg attactcggt tttctcgagc gtttctacgc atgctcctgc
     2761 cgctcgccgc agccgaacgg caggagcgta gcgactgagt gagcgaggaa gcggaaaaga
     2821 ggagatccct acactgacca ctgacgctct gaggcctctt atgacgaaac aacagaaaac
     2881 agcgctgaac atggctaaat tcatccagaa tcagtctctg ctcctgctgg agaagcttaa
     2941 cgagcttgat ctcgatgtcg aggccgacct ttgtgagaaa ctccacgatg atgcggagca
     3001 tcttttccgt acactgtcct cgagactgga tgcattacag gatggtaact gatggccaga
     3061 gtgtctatta gtgaggctgc ccgactgacg ggtaagagta gaacaacact gcacaggtta
     3121 attaaagcag gtgacctgtc cacctgtagc ggtgagcgca atgcaaaaat ggtagatact
     3181 tcagagcttt tacgtgtctt tggccccttt gagcaaccga agggtgaaca ccattctgga
     3241 caggtctctg gacagcgtga tacagcctct gctgaccaga gtgaacaggt gatccgtcat
     3301 ctgaaacagg agattgaaca cctgaacacc ttacttttgt ccaaggattc gcacattgac
     3361 agcctgaaac aggccatgct ccttcttgaa aatcaccagg caaaattaac tgaatccccg
     3421 gcgccatggt ggaaattctg gaaaaaatcc tgaccccgtc atgcttcagt tttcctctcc
     3481 ggccatccca gggagttcct gaaccttccc aaaaatcgtc agatctctga acgctcctca
     3541 ccactaaaaa tacccacacc ccacgcaaaa ttaaaatttt gcagattcct tatttacaac
     3601 aatgggttaa tgaaaaataa atgtgcggtg atgtgttaca aagtgcagat ccaagtcgtg
     3661 gacgactcta tggcgaatcg ctgacgaatt gcccacgatt catatgtgaa tcacgcttta
     3721 ttatcatatt gttatgatga atcgactctt gattcaattc atcactttgg gtgttttttg
     3781 ctcatggtga ctcttgattc aattcacgat tcgaatcgcc ggtgaatcgt gtggtgagtc
     3841 gtccggcgac ttgccataac gtgattgccg gccgtcgacg aacccgattc ctgaaggtgc
     3901 tgaaaaccgg ttttccggct gcgccggcaa cgcttcacgg ccacgcaata aattgcgtcc
     3961 ccgttcaccg ttttcggagg agaagaacgc cggaaacagg acttcccctg gctgaatggt
     4021 gtgaatttcc gcgctatgca cttgcgcgca tactcatgca tgccgtaaaa acagagcctg
     4081 cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt taccggtttc ccgtcagaaa
     4141 cgtcctgagt ccgttttagc ggtgcgt
//
