LOCUS       NZ_CP140508             3174 bp    DNA     circular CON 06-JAN-2024
DEFINITION  Escherichia coli strain 2023CK-01341 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP140508
VERSION     NZ_CP140508.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38251294
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
REFERENCE   3  (bases 1 to 3174)
  CONSRTM   Clinical and Environmental Microbiology Branch: Whole genome
            sequencing antimicrobial resistance pathogens in the healthcare
            setting
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2023) Division of Healthcare Quality Promotion, US
            Centers for Disease Control and Prevention, 1600 Clifton Rd,
            Atlanta, GA, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: Unicycler v. 0.5.0
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 20.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/06/2024 05:17:55
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 4
            CDSs (total)                 :: 4
            Genes (coding)               :: 4
            CDSs (with protein)          :: 4
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140508.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="2023CK-01341"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2023-10-18"
     gene            complement(join(3039..3174,1..293))
                     /locus_tag="U4W76_RS00005"
                     /old_locus_tag="U4W76_24530"
     CDS             complement(join(3039..3174,1..293))
                     /locus_tag="U4W76_RS00005"
                     /old_locus_tag="U4W76_24530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            585..1013
                     /locus_tag="U4W76_RS00010"
                     /old_locus_tag="U4W76_24540"
     CDS             585..1013
                     /locus_tag="U4W76_RS00010"
                     /old_locus_tag="U4W76_24540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     gene            complement(1849..2043)
                     /locus_tag="U4W76_RS00015"
                     /old_locus_tag="U4W76_24545"
     CDS             complement(1849..2043)
                     /locus_tag="U4W76_RS00015"
                     /old_locus_tag="U4W76_24545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001485273.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2327..2650
                     /gene="mobA"
                     /locus_tag="U4W76_RS00020"
                     /old_locus_tag="U4W76_24550"
     CDS             2327..2650
                     /gene="mobA"
                     /locus_tag="U4W76_RS00020"
                     /old_locus_tag="U4W76_24550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052584083.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQVIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     Typing          2079..2318
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(1289..1814)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.03"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          2328..2647
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="2.92e-61"
ORIGIN
        1 cggcgatcac tcttcgatgt tggtttcttg tccaagagat tagctttttc aagatcattg
       61 atagctctct gaacagtccg tacagaaacc cccatacgta tggctagact ttccattgac
      121 ggatgcggcc actcttgcaa actccaccag tgaacgatca ggttaagtag tgtgttaaag
      181 gccactgaag ttagcttttt ctcgttttgt ataaaaaaca atacggtagg cactgctgtc
      241 cagccaagag acaaaccgcc agctttccat ttattcttaa cggagtaagt cattgatttt
      301 cctaagcccc aaaatattta aagtatatat tatatggtat attcatatga atagggtgac
      361 actagcgcca ttattgtgca accaaaaaag actactctga aaacgaggaa aagatttttt
      421 cctgcctgaa ttagatacgg agttagcgat atgaaaaccg aacaacgtca tgatcttgtt
      481 aaagatattg aggtttttgg cgtatccttg tctctgttga tttccagagc gaatgagaag
      541 tctgttacaa tgccatctgg tctaagtcgg gagcagagaa gagcatgggc agcggagcag
      601 gcgcgcaaaa tccacaattg aatattgtct cattctctga gaccttcaac ctttattaca
      661 catccagata ttctgcaaaa acactcgata aaatcgatga tttcattgag cattttgaaa
      721 aatacaatct ctttggcgat cctttaaaag gatatccagc ttggactggc aaagtatcgc
      781 catcgtggaa agtgcctgat cattacgaaa acaaagaagc tattgagaag tatgctagag
      841 ctaacaaatt atggcatgct catttaggcg atccggtttt taaagatacg tttcatggga
      901 aatacaaggt ttctgactgg gttattcatt tccagcggct gacaccgaac catataaagc
      961 ttttagagct tggataccat gacccaatga agctacctca aaactttgaa tgatcgagcg
     1021 gcaggctaaa tgaaatcttg agattcattc agtctcgtcg taatctcact attgtaaaaa
     1081 cgaaaaaacc accctggcag gtggtttttc gaaggttagt taatcctggc agattctcta
     1141 accgtggtaa cagtcttgtg cgagacatgt caccaaatac tgtcctttca gtgtagcctc
     1201 agttaggccg ccacttcaag aactctcgtt acatctctcg cacatcctgc ttaccagtgg
     1261 ccgttgccag tggcgttaag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1321 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1381 cgacctacac cgaaccgaga taccaacagc gtgagctatg agaaagcgcc acgcttcccg
     1441 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1501 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1561 gacttgagcg tcggtttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1621 acggcgcggc ctttgtccct ggcttcggct ttttgctcac atgttctttc cggcgttatc
     1681 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgacaccg ctcgccgcag
     1741 tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagagcgcc ttatgtgaca
     1801 ttttctcctt acgctcctct atgccgttct gcatcctgtc cggatgcgtt atatcccggt
     1861 aagattttcc gcttcaaagc gtgtctgtat gctgttctga agttcttccg cgagttcgtg
     1921 cagcttctca cacatggcgg cctgttcgtc agcatcaagt gcgtccagtt tttcgagcag
     1981 cgtcaggctc tggcttttta tgaatcctgc catgttgagt acggtttgct gctgcttgtt
     2041 catctttctg ttttctccgt tctttctgtc atctgcgttg tgtgattata tcgcgtacca
     2101 cttttcgact gttttaccgt cggtattctg ctgtctgact ctttgacggg catttctgtc
     2161 agacaacact gtcactgcca aaaaactgcc gtgcctttgt cggtaattcg aacttgctga
     2221 caggacagga tgtgtaattg ttataccgcg catacatgca cgctattaca attaccctgg
     2281 tcagggcttt gccccgacac ccatgtcaga tacggagcca tgttttatga caaaacgaag
     2341 tggaagtaat acgcgcaggc gggctatcag tcgccctgtt cgtctgacgg cagaagaaga
     2401 ccaggtaatc agaaaaaggg ctgctgaatg cggcaagacc gtttccggtt ttttacgggc
     2461 ggcagctctc ggtaagaaag ttaactcact gactgatgat cgggtactga aagaagttat
     2521 gagactgggg gcgttacaga aaaaactctt tatcgacggc aagcgtgtcg gggacaggga
     2581 gtatgcggag gtgctgatcg ctattacgga gtatcaccgt gccctgttat ccaggcttat
     2641 ggcagattag cttcccggag agaaaactgt cgaaaactga cggtatgaac accgtaagct
     2701 cccaaagtga tcaccattcg ctttcatgca tagctatgca gcgagctgaa aacgatcctg
     2761 acgcatcctt cctgtttttc cggggtaaaa catctctttt tgcggtgtct gcgtcagaat
     2821 cgcgttcagc gcgtttcagt ggtgcgtaca attaagggat tatggtaaat atatgagcta
     2881 tgcgataact ttaactgtga agcgatgaac ccattacagg caaagccaat tactcctgac
     2941 agtggtttag ccagaagcag ggctaccaag accaatgcaa taagtaatat atcgttttgc
     3001 tatcgtgcat cgctcgcgct cagttccatt gtgctttttt aagctgtcgt ttttcttacg
     3061 gtatataccg gttttttatg gcgtggtttc ttaacttgtt cagctactga tggacccatt
     3121 gtatctaggt agtcaactag ctttgttaga tcataaatat tgcgaccacc atat
//
