LOCUS       NZ_CP140503             4261 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 2023CK-01337 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP140503
VERSION     NZ_CP140503.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38122037
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 20.0x
            Sequencing Technology :: Illumina MiSeq; Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 10:29:27
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5
            CDSs (total)                      :: 5
            Genes (coding)                    :: 4
            CDSs (with protein)               :: 4
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 1 of 1
            Pseudo Genes (incomplete)         :: 0 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140503.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="2023CK-01337"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2023-10-09"
     gene            complement(679..1101)
                     /locus_tag="U4W17_RS00005"
                     /old_locus_tag="U4W17_27905"
     CDS             complement(679..1101)
                     /locus_tag="U4W17_RS00005"
                     /old_locus_tag="U4W17_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_039817119.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSFTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGVNSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQFLEHKHEPSSPSDSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1101..1292)
                     /locus_tag="U4W17_RS00010"
                     /old_locus_tag="U4W17_27910"
     CDS             complement(1101..1292)
                     /locus_tag="U4W17_RS00010"
                     /old_locus_tag="U4W17_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_039817117.1"
                     /translation="MTKQQKTALNMAKFIQHQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1708..1850
                     /locus_tag="U4W17_RS00015"
                     /old_locus_tag="U4W17_27915"
                     /pseudo=""
     CDS             1708..1850
                     /locus_tag="U4W17_RS00015"
                     /old_locus_tag="U4W17_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2059..2262)
                     /locus_tag="U4W17_RS00020"
                     /old_locus_tag="U4W17_27920"
     CDS             complement(2059..2262)
                     /locus_tag="U4W17_RS00020"
                     /old_locus_tag="U4W17_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427298.1"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="WP_072040881.1"
                     /translation="MEKGFINFYDHVRGYGFIRRECGRDVFFKHDDFFDFESSVDVCKG
                     IFVNFEVIKADKGLRAVNIRIV"
                     /name="cold shock domain-containing protein"
     gene            complement(2267..3406)
                     /locus_tag="U4W17_RS00025"
                     /old_locus_tag="U4W17_27925"
     CDS             complement(2267..3406)
                     /locus_tag="U4W17_RS00025"
                     /old_locus_tag="U4W17_27925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761319.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="WP_072040880.1"
                     /translation="MNKPRFNGTPIASLDSLAAMLGIDRKRLDWMVLSVSRSYKQFTVE
                     TGKNKKERKIFEPKRSLKGIQKKINKEIFENIDYPHYLHGAITGRDYISNASVHTKKKT
                     IICLDITNFYPSISKRDVTLIFKNLMRFSPDVSIALTELVTLNNQVPQGGCCSSYIANL
                     LFFNSEYNLYNRLQSIGYSYTRLLDDITISSDEDISLEEKTKVIQLVYRMVSQHRLSIN
                     EDKTTIEHTKDSSSKLSVTGLWVKHGVPKLTKENRRYIRYLVYICQKQAAYERHTKEYH
                     DLWNRCSGKVAQMSRLGHVQANELRCVLSDILPIYDDYKIGKLKYVAKHYLSKFTHPLS
                     EEQIRKVDRMIYDFDIVGRTNEKLAKDYRSKLRALLPER"
                     /name="reverse transcriptase family protein"
     Typing          1414..1817
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.089"
                     /coverage="99.0"
                     /evalue="9.84e-174"
ORIGIN
        1 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt
       61 ttttacggca tgcatgagta tgcacgcaag tgcatagcgc gaaaattcac gccattcagc
      121 cagagtaagt cctgtttccg gcgttctttc catccgaaaa cggtgaacgg agacgcaatt
      181 tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca gcacgttcag
      241 gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa gtcgccggac gactcaccac
      301 acgattcacc ggcgactctt cactagagtc gtacccagag tcgccgcgtc taaaaaacaa
      361 ccaaacgggg agtcgtgtct agagtcgggt cgttattaac ttattgtttt tatagtcctg
      421 agtcgttacc gactctatga cgactcgccg gggactctat gacgagtcgt catagagtcg
      481 gatctatata aataaaattt aaaaaacgaa atttcaggaa gttaacccac tgatattaaa
      541 tacaaatatg caaaatgtta attttgcgta gtgtgtgggt atttttagtg gtgaggagcg
      601 ttcagagatc tgacgatttt tgggaaggtt caggaactcc ccgggatggc cggagaggaa
      661 aacggaagca tgacggggtc aggatttttt ccagaatttc caccacgggg aatcggatgg
      721 tgaagaaggt tcgtgcttgt gctccagaaa ctgcattgcc tgacgaaggg aatcaatatg
      781 ctcatctcga gccttaacag cccctttaag accatcgttt tcagcttcaa gcaaagcaat
      841 tctatgttgt aaatcatgta aaccaacgct gttaacacct gtagattctg cgtcactttt
      901 tacatcatat tttacagtat cgatgacacc atcgagagaa ccaaacactc taataagctc
      961 tgaagtatca ataactttaa tagccttccc gtgctttaca acagaggtga aacttaactt
     1021 cccactgtta atcatcttgt aaattgtggg gcgactaacc ttaacaagcc gtgccgcttc
     1081 actaatagac actctggcca tcagttacca tcctgtaatt catccagtct cgaggacagt
     1141 gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcagc ctcagcatca
     1201 agatcaagct cgttaagctt ctccagcagg agcagagact gatgctggat gaatttagcc
     1261 atgttcagcg cggtcttctg ttgtttcgtc ataagaggcc tcagagcgtc agtgctcagt
     1321 gtagggatgt tctcttttcc gcttcctcgc tcactcagtc gctatgctcc tgccgttcgg
     1381 ctgcggcgag cggcaggagc atgcgtataa aagctcgaga aaaccgagcg gtcgcgtgac
     1441 ggtgaaaagg aaaggccgct aagcggccgt ttttccatag gctccgcccc cctgacaagc
     1501 atcacagaaa ctgacgctca aatcagtggt ggcgaaaccc gacaggacta taaagatatc
     1561 aggcgtttcc ccctgatggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     1621 gtgtcattcc gctgttatgg ccgcgtttat ctcattccac gcctgacact cagttccggg
     1681 aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgactgctgc
     1741 gccttttcgg taactatcaa cttgagtcca acccgaaaag acacgactta tcaccactgg
     1801 cagcagccac tggtaacgca ttcgcagaac aaagaagtgt tgaagtgtga ggtaaactac
     1861 agctacactg ttagaacgaa ttcggtttct gtgctctgcg aaagccagtt accacggttc
     1921 agaaacttgc cggttaatga accttcgaaa aaaccacctt gccgggtggt tttttcgttt
     1981 acagggcaag agattacgac gagcgaggta ggatctcaag agatcatttc atcaaaaagg
     2041 ctcaaaaacc tctatatttt atacaattcg tatatttaca gccctaagcc ctttatctgc
     2101 ttttattact tcaaaattaa cgaaaatccc cttacataca tcaacactac tttcaaaatc
     2161 aaaaaaatca tcatgcttga aaaaaacgtc acgcccacac tcccttcgta taaaaccata
     2221 tcccctaaca tggtcgtaaa agttaataaa ccctttttcc atataatcac ctctcaggaa
     2281 gcaacgctct aagcttactt ctatagtctt ttgctagttt ttcattagtt cgaccaacta
     2341 tatcaaagtc atagatcatt ctatcaactt ttcttatttg ctcttcactc aaagggtgcg
     2401 taaacttact taaataatgc ttagccacat attttaactt acctatttta tagtcatcat
     2461 aaataggcaa tatatcagaa agaacgcacc ttaactcatt agcttgaaca tgcccaagcc
     2521 gagacatttg tgcaaccttc cctgaacatc tattccataa atcatgatac tctttcgtat
     2581 gccgctcata agcagcttgc ttttggcata tatacaccaa atatcttata tatcgtctat
     2641 tctcttttgt taatttagga acaccatgct tcacccacaa cccagtgacg ctaagcttcg
     2701 aagaggagtc cttggtgtgc tctattgtcg ttttatcttc atttatggat agcctatgct
     2761 gagaaaccat tctatagacg agttgaatta cttttgtttt ctcttctaat gaaatatcct
     2821 catcagagga tattgtaata tcatctaaca accttgtgta tgaataccca atagattgca
     2881 atctattata aagattatac tcactattaa agaacaaaag atttgcaatg taactagagc
     2941 aacaccctcc ttgcggaact tgattattca atgtcacaag ctcagttaaa gcaatagata
     3001 catcaggaga gaatcgcatt aaatttttaa atataagagt aacatcccgc ttactaatgc
     3061 ttggataaaa attagttata tctagacata ttattgtttt tttctttgtg tgcacggatg
     3121 catttgatat atagtcacgc ccagttatcg ccccatgcaa ataatgaggg taatctatat
     3181 tttcaaatat ttctttattt attttctttt gaataccctt taaagatctc ttgggctcaa
     3241 atatttttct ttctttttta ttttttcctg tttcaacagt aaactgcttg tatgacctag
     3301 atactgataa gaccatccag tccaatcttt tcctgtctat acctaacatc gcagccaaag
     3361 aatccaagga tgctataggt gttccattaa acctaggttt attcatgatg tttgagttaa
     3421 aagtcgatta gttaaagaat tttgatatat aatctaataa tttaacaaaa atcaagaaca
     3481 tacaacccca aaactgcggg gttttcagca cttccagaga gccaacctct ctttttttct
     3541 tcttctgtcc gtctccggaa caagatgaag agctcttggt tacgtaaatg ttgatgttga
     3601 aaataaacat agtactgctc ctagtggttg tgaaattttg ccagaccata ggaaagcaag
     3661 gaaaaattct attggctctt tgggactttt aactcacatt gccagacaat cgtcgccatt
     3721 tttgaagcga taatgagcgc tcaggggcgt tcttaccgtt aatgggcggc aaggccggtc
     3781 atgatctcat gtgagatcgc cttaaacaac aaggcatcgg tttccttgac acaacataaa
     3841 gcgtaaccat tttttaaaga gcgagagcca ggcgtggttc gcccgcttct ctgattatta
     3901 taaagttttt tatgatgctg tcatcaaaga aaacgaacaa gatgaaaatc cctcgtatag
     3961 agggcctgaa tcatcattac gtataaactt cagaataaac gagcagcaaa agccacgaca
     4021 acacccccta cactcccgtt aacattcgca atggttctca ggcgatcatc cttgagaggc
     4081 ttgccgtcaa aggtcgcaat agttcttaag cgatcatctt ttatagttac actcacaaat
     4141 actcaataat aagttaactt tctgaatttc ctaagattaa atgaaaatgg catacaaccc
     4201 catttaacat aaggccatga ttgtacgcac cgctaaaacg gcctcagggc gtttctgacg
     4261 g
//
