LOCUS       NZ_CP140479             8822 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain FF29-001BS plasmid
            Col440II, complete sequence.
ACCESSION   NZ_CP140479
VERSION     NZ_CP140479.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38451788
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8822)
  AUTHORS   Manyahi,J.
  TITLE     bla NDM5 Klebsiella pneumoniae Tanzania
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8822)
  AUTHORS   Manyahi,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2023) Clinical Science, University of Bergen,
            Haukelands veien 208, Bergen 5020, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 10:24:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 16
            CDSs (total)                      :: 16
            Genes (coding)                    :: 12
            CDSs (with protein)               :: 12
            Genes (RNA)                       :: 0
            tRNAs                             :: 0
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 2 of 4
            Pseudo Genes (incomplete)         :: 2 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140479.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8822
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FF29-001BS"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="Col440II"
                     /geo_loc_name="Tanzania: Dar es Salaam"
                     /lat_lon="6.8165 S 39.2894 E"
                     /collection_date="2022-03-03"
                     /collected_by="Joel Manyahi"
                     /genotype="ST437"
     gene            448..819
                     /locus_tag="SNM26_RS00005"
                     /old_locus_tag="SNM26_28720"
     CDS             448..819
                     /locus_tag="SNM26_RS00005"
                     /old_locus_tag="SNM26_28720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021314598.1"
                     /translation="MSKKNDLNLSESTLESLREYVDDFGMTVDDVVLKLINENKSLKES
                     LGQDFVLSGEKEAHHFKDYVIHKLPKGKILVTHNGLKASNMKKILIEAGRELGMDENEL
                     NPDFTTYEIGRFLFKHLHA"
                     /name="hypothetical protein"
     gene            complement(916..1341)
                     /locus_tag="SNM26_RS00010"
                     /old_locus_tag="SNM26_28725"
     CDS             complement(916..1341)
                     /locus_tag="SNM26_RS00010"
                     /old_locus_tag="SNM26_28725"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046181484.1"
                     /translation="MSFQQVLNEVKVISSNGDTSLAITRYDQQGLNRFLLRDLSHQTET
                     RSNYVNNLEQLCQQAKCNRVILNVNEISESFYMKLITFCNFTLLDAKQDMQHLNGFNYF
                     IVLNSFDENSGHTVWAPSYSPNCVTERDLDEYDPDRW"
                     /name="hypothetical protein"
     gene            complement(1354..1608)
                     /locus_tag="SNM26_RS00015"
                     /old_locus_tag="SNM26_28730"
     CDS             complement(1354..1608)
                     /locus_tag="SNM26_RS00015"
                     /old_locus_tag="SNM26_28730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101361146.1"
                     /translation="MNITINTPSVKTILDVQCDHCNFTGTIDYEAPRISKLTVGGKITF
                     DNALCPQCKTGEIFAPGGQYVRDDATGRMNRTGDANISL"
                     /name="hypothetical protein"
     gene            complement(1863..1996)
                     /locus_tag="SNM26_RS00020"
                     /old_locus_tag="SNM26_28735"
                     /pseudo=""
     CDS             complement(1863..1996)
                     /locus_tag="SNM26_RS00020"
                     /old_locus_tag="SNM26_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            2616..2957
                     /locus_tag="SNM26_RS00025"
                     /old_locus_tag="SNM26_28740"
     CDS             2616..2957
                     /locus_tag="SNM26_RS00025"
                     /old_locus_tag="SNM26_28740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3166..>3594
                     /locus_tag="SNM26_RS00030"
                     /old_locus_tag="SNM26_28745"
                     /pseudo=""
     CDS             3166..>3594
                     /locus_tag="SNM26_RS00030"
                     /old_locus_tag="SNM26_28745"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3635..4132
                     /locus_tag="SNM26_RS00035"
                     /old_locus_tag="SNM26_28750"
     CDS             3635..4132
                     /locus_tag="SNM26_RS00035"
                     /old_locus_tag="SNM26_28750"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            4139..4354
                     /locus_tag="SNM26_RS00040"
                     /old_locus_tag="SNM26_28755"
     CDS             4139..4354
                     /locus_tag="SNM26_RS00040"
                     /old_locus_tag="SNM26_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            4859..5230
                     /locus_tag="SNM26_RS00045"
                     /old_locus_tag="SNM26_28760"
     CDS             4859..5230
                     /locus_tag="SNM26_RS00045"
                     /old_locus_tag="SNM26_28760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021314598.1"
                     /translation="MSKKNDLNLSESTLESLREYVDDFGMTVDDVVLKLINENKSLKES
                     LGQDFVLSGEKEAHHFKDYVIHKLPKGKILVTHNGLKASNMKKILIEAGRELGMDENEL
                     NPDFTTYEIGRFLFKHLHA"
                     /name="hypothetical protein"
     gene            complement(5327..5752)
                     /locus_tag="SNM26_RS00050"
                     /old_locus_tag="SNM26_28765"
     CDS             complement(5327..5752)
                     /locus_tag="SNM26_RS00050"
                     /old_locus_tag="SNM26_28765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046181484.1"
                     /translation="MSFQQVLNEVKVISSNGDTSLAITRYDQQGLNRFLLRDLSHQTET
                     RSNYVNNLEQLCQQAKCNRVILNVNEISESFYMKLITFCNFTLLDAKQDMQHLNGFNYF
                     IVLNSFDENSGHTVWAPSYSPNCVTERDLDEYDPDRW"
                     /name="hypothetical protein"
     gene            complement(5765..6019)
                     /locus_tag="SNM26_RS00055"
                     /old_locus_tag="SNM26_28770"
     CDS             complement(5765..6019)
                     /locus_tag="SNM26_RS00055"
                     /old_locus_tag="SNM26_28770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101361146.1"
                     /translation="MNITINTPSVKTILDVQCDHCNFTGTIDYEAPRISKLTVGGKITF
                     DNALCPQCKTGEIFAPGGQYVRDDATGRMNRTGDANISL"
                     /name="hypothetical protein"
     gene            complement(6274..6407)
                     /locus_tag="SNM26_RS00060"
                     /old_locus_tag="SNM26_28775"
                     /pseudo=""
     CDS             complement(6274..6407)
                     /locus_tag="SNM26_RS00060"
                     /old_locus_tag="SNM26_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            7027..7368
                     /locus_tag="SNM26_RS00065"
                     /old_locus_tag="SNM26_28780"
     CDS             7027..7368
                     /locus_tag="SNM26_RS00065"
                     /old_locus_tag="SNM26_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            7577..>8005
                     /locus_tag="SNM26_RS00070"
                     /old_locus_tag="SNM26_28785"
                     /pseudo=""
     CDS             7577..>8005
                     /locus_tag="SNM26_RS00070"
                     /old_locus_tag="SNM26_28785"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            8046..8543
                     /locus_tag="SNM26_RS00075"
                     /old_locus_tag="SNM26_28790"
     CDS             8046..8543
                     /locus_tag="SNM26_RS00075"
                     /old_locus_tag="SNM26_28790"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            8550..8765
                     /locus_tag="SNM26_RS00080"
                     /old_locus_tag="SNM26_28795"
     CDS             8550..8765
                     /locus_tag="SNM26_RS00080"
                     /old_locus_tag="SNM26_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          2347..2522
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="82.32"
                     /coverage="100.0"
                     /evalue="5.910000000000001e-33"
     Typing          6758..6933
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="82.32"
                     /coverage="100.0"
                     /evalue="5.910000000000001e-33"
     Typing          complement(1885..2401)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.962"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(6296..6812)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.962"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2948..4470
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7359..8821
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.59"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagatctggt accggaacgg caacgcacat tatctcatgg tgacagggag tatggagact
       61 gataaacgcc cgtctcaggt ggcctgagcg tgacggggat catggccatt ttctgcgatt
      121 tcagcacaca gagagtcttt tttaaccgaa atcaccggct atgcagtgat gtgaataagt
      181 gatgcatatg cgtaaaataa atcaggtgcg tttctggtgg ttttcggtgt acttgccacc
      241 accttttgcc cgtcagccat cagaaacgcc ctgagtgcct ccggcgcggt gcgggtaaga
      301 gatgttatgg taaatccagc ggtgtagctg ttctttttga accagcgatc agtgacttct
      361 atgttgcttg ttcaatacta aaaatatgtg tactatacta cttgtgttgt ttttttcttt
      421 tctgacaact agcatagtgg tgactacatg agtaaaaaaa acgatttaaa cctcagtgaa
      481 tcaacgttag aaagcctcag ggagtatgtc gatgacttcg gcatgactgt tgatgatgtt
      541 gttctgaagt taattaatga gaataagtca ttgaaggaat cacttggaca agactttgtg
      601 ttatctggcg aaaaggaagc gcaccacttt aaagattatg tgattcataa actgcccaaa
      661 ggaaaaattc tggttaccca taatgggctt aaggccagca acatgaagaa gatattgatt
      721 gaggcgggga gggagctagg catggatgaa aatgaactca atccagattt cacaacctat
      781 gagatagggc gttttttgtt taagcaccta catgcgtaac aatcctgcac gcctgaaggg
      841 caggaatcga acttagctgc ccggatgtta tggtaaattt aggtgctata cggatttttt
      901 attaggcgat ctgagttacc aacgatctgg gtcgtattca tctaaatcgc gctcagttac
      961 acaattgggg ctatacgatg gtgcccaaac tgtatgccca ctgttttcat caaaagaatt
     1021 aagaacaata aagtaattga agccattcaa atgttgcata tcttgtttgg cgtctaacag
     1081 agtgaaatta caaaaagtta ttaatttcat ataaaacgac tctgaaattt cattcacgtt
     1141 caaaataacc ctgttacatt ttgcctgctg gcataattgc tcaagattat ttacatagtt
     1201 actacgagtc tcggtctgat gagataagtc acgtaaaaga aaacggttca atccttgttg
     1261 gtcatatctg gtaatcgcta ggcttgtatc accattgctt gaaataacct ttacctcatt
     1321 caaaacttgc tgaaaactca tatgataagt tccttataga ctaatgttcg catcgcctgt
     1381 gcgattcatt cttccagtag catcatctct aacgtattga ccacctggag cgaaaatttc
     1441 acctgtttta cattgcgggc ataaagcatt gtcgaaggtt atttttccgc cgacagtaag
     1501 ctttgaaatt cgaggggctt cataatcaat agtgccagtg aagttacagt gatcacactg
     1561 tacatcaagg atggttttta ctgacggggt attgatagtt atattcattc tcaacaatcc
     1621 ttatcattac aagtggttgt gatgacttta tcagagaatg cttagctaat gaaaacgaag
     1681 gatcttcttg agatcgtgtt cctctcgtcg tagtcttcat tcctgtaaac gaaaaaaccg
     1741 ccttgctggg cggttttttc gaaggttctt ggagcagcaa ctctttgaac cgaggtaact
     1801 ggcttggagg agcgcagtca ccaaatctgt cctttcagtt tagccttaac cggcgcataa
     1861 cttcaagact caactcctct aaatccgtta cctgtggccg ttgccattgg cgctttgtat
     1921 gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa
     1981 cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca
     2041 ggcgtggaat gagaaaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca
     2101 ggaacaggag agcgcacgag ggagctacca gggggaaacg cctgggatct ttatagtcct
     2161 gtcgggtttc gccaccactg atttgagcat cagattctgt gatgctcgtc aggggggcgg
     2221 agcctatgga aaaacggcag cgccgcgtcc ttttctcttc cttgccttca cggcatatgt
     2281 ctctgcattt ctccgcgtgc cggttacgcc cgccgcagcc gaacggcagg agcgtcagcg
     2341 accgagtgag cgaggaggcg tcatatctcc tgcacaacca ttatctgtct tacgcccggc
     2401 agctgcatgt catacctgcc atatatagca ctttcgctga tacccatacc aatgccaaca
     2461 tagtaagcca gtatacactc cgctagcgct acgtgactgg ttcagggctg cgccccgaaa
     2521 cccgctaaaa gccactgacg cgcctgcggc ttgtccaacc ccgcgccgac cgggaaaggc
     2581 tttcccgtcc gtcacggcgt tatcaggagg cgattctggc agataaacgc aacaagatgc
     2641 tcacgatgtg ggtgacggaa gacgaacacc ggcggttgct tgagcggtgc gacggaaaac
     2701 aactggcggc gtggatgcgc cagacctgcc ttgatgagaa accgacacgc gccggcaggc
     2761 ttccctcgat ttcgcccgcg ctgctgcgtc agcttgctgg catgggcaat aacctgaacc
     2821 agatcgcccg gaaagtgaac gccggtggtg ccggtcatga ccgggtgcag atcgtggcag
     2881 cgctgatggc gatcgatgcc ggtctggaac ggttgcggca tgccgtactg gaaaaagggc
     2941 cggacgatga ttgttaaatt ccatccccgg ggtcgcggcg ggggcgccgg tcctgtggat
     3001 tatcttctgg gtaaagaccg gcagcgtgaa ggtgccagcg tcctgcaggg gaagccggaa
     3061 gaagtccggg agctgattga tgcgtcgccg tatgtgaaaa aatatacctc gggggtgctg
     3121 tcattcgcgg aggcggacct gccgccgggt cagcgtgaaa aactgatggc cagctttgag
     3181 cgggttctga tgcccggact cgataaagat cagtacagca ttctgtgggt ggaacacgca
     3241 gacaagggtc gtcttgagct gaattttctg atccccaata ccgaattact gacggggaaa
     3301 cgcctccagc cgtattacga tcgtgccgac cggccgcgca tcgatgcgtg gcagacagtg
     3361 gtcaacggca ggctggggct acatgatccg aacgacccgg agaaccggcg ggcgctggtc
     3421 acgccgtcag gactgccaga aacgaagcag gaagccgcag agacgattac acggggtttg
     3481 ctgatgttag cctcatcagg agagctgaaa acgcgtgatg acgtcacagg ggcgctcaca
     3541 gcggccggtt ttgaggtggt cagaaccaca aaaaacagta tcagcattgc cgacccggac
     3601 ggggggcgaa atatccgact gaggggagcc atttatgagc agtcttttaa cggcctcgag
     3661 ggacttcgag gagaaatcga aagcacagca gcagcttacc gaagagaggc tgaaagccgc
     3721 attcagcaag cacgagaacg ttgtcaaatc ggaactgaac gcaagcgcga agagaatcaa
     3781 cgacgccatc agcgcccacg aacaggggat gaacgcggcg atgcagtcga accggctgag
     3841 cgtgctgcga atggtcgggc ggacgtggct gaccatcacc atggtgtcag ggctgctgtt
     3901 cgccagcctg agcggggtgc tgtggtacca ggggaaccgg atagcggcga acctggcgga
     3961 aatcaggcag cagagagata cgctgtcgaa actgcagatg cagacgtggg gcgtgaccta
     4021 tctggagaac cgcaacggac ggtttctggt gctgccggag gggatgaaag ccgaaacggg
     4081 ccggacggtg gacaacaaga cgcggaacgc ggtgaagctg gtacgggagt agagcagaat
     4141 gacggaactg gaaaaacaat tgctgagcgc gttagagcag ctacagcagg actactcgag
     4201 aaggctggac gagtgggaga gcgccttcga ggaatggcgg aagatgtctg gtcttatgca
     4261 acgggagaac gcggcgctga gcgagcgcgt gtcgggcttg agcaggcagg tgcagagttt
     4321 gagcgagcag ctgcgccggt tatcgacagg ctgaatgaga ttgagcaaca gcgatacggg
     4381 caaagaacgg aaaaggaacc agagaagaca gcagatctgg taccggaacg gcaacgcaca
     4441 ttatctcatg gtgacaggga gtatggagac tgataaacgc ccgtctcagg tggcctgagc
     4501 gtgacgggga tcatggccat tttctgcgat ttcagcacac agagagtctt ttttaaccga
     4561 aatcaccggc tatgcagtga tgtgaataag tgatgcatat gcgtaaaata aatcaggtgc
     4621 gtttctggtg gttttcggtg tacttgccac caccttttgc ccgtcagcca tcagaaacgc
     4681 cctgagtgcc tccggcgcgg tgcgggtaag agatgttatg gtaaatccag cggtgtagct
     4741 gttctttttg aaccagcgat cagtgacttc tatgttgctt gttcaatact aaaaatatgt
     4801 gtactatact acttgtgttg tttttttctt ttctgacaac tagcatagtg gtgactacat
     4861 gagtaaaaaa aacgatttaa acctcagtga atcaacgtta gaaagcctca gggagtatgt
     4921 cgatgacttc ggcatgactg ttgatgatgt tgttctgaag ttaattaatg agaataagtc
     4981 attgaaggaa tcacttggac aagactttgt gttatctggc gaaaaggaag cgcaccactt
     5041 taaagattat gtgattcata aactgcccaa aggaaaaatt ctggttaccc ataatgggct
     5101 taaggccagc aacatgaaga agatattgat tgaggcgggg agggagctag gcatggatga
     5161 aaatgaactc aatccagatt tcacaaccta tgagataggg cgttttttgt ttaagcacct
     5221 acatgcgtaa caatcctgca cgcctgaagg gcaggaatcg aacttagctg cccggatgtt
     5281 atggtaaatt taggtgctat acggattttt tattaggcga tctgagttac caacgatctg
     5341 ggtcgtattc atctaaatcg cgctcagtta cacaattggg gctatacgat ggtgcccaaa
     5401 ctgtatgccc actgttttca tcaaaagaat taagaacaat aaagtaattg aagccattca
     5461 aatgttgcat atcttgtttg gcgtctaaca gagtgaaatt acaaaaagtt attaatttca
     5521 tataaaacga ctctgaaatt tcattcacgt tcaaaataac cctgttacat tttgcctgct
     5581 ggcataattg ctcaagatta tttacatagt tactacgagt ctcggtctga tgagataagt
     5641 cacgtaaaag aaaacggttc aatccttgtt ggtcatatct ggtaatcgct aggcttgtat
     5701 caccattgct tgaaataacc tttacctcat tcaaaacttg ctgaaaactc atatgataag
     5761 ttccttatag actaatgttc gcatcgcctg tgcgattcat tcttccagta gcatcatctc
     5821 taacgtattg accacctgga gcgaaaattt cacctgtttt acattgcggg cataaagcat
     5881 tgtcgaaggt tatttttccg ccgacagtaa gctttgaaat tcgaggggct tcataatcaa
     5941 tagtgccagt gaagttacag tgatcacact gtacatcaag gatggttttt actgacgggg
     6001 tattgatagt tatattcatt ctcaacaatc cttatcatta caagtggttg tgatgacttt
     6061 atcagagaat gcttagctaa tgaaaacgaa ggatcttctt gagatcgtgt tcctctcgtc
     6121 gtagtcttca ttcctgtaaa cgaaaaaacc gccttgctgg gcggtttttt cgaaggttct
     6181 tggagcagca actctttgaa ccgaggtaac tggcttggag gagcgcagtc accaaatctg
     6241 tcctttcagt ttagccttaa ccggcgcata acttcaagac tcaactcctc taaatccgtt
     6301 acctgtggcc gttgccattg gcgctttgta tgtctttccg ggttggactc aagacgatag
     6361 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     6421 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagaaaaac gcggccataa
     6481 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagctacc
     6541 agggggaaac gcctgggatc tttatagtcc tgtcgggttt cgccaccact gatttgagca
     6601 tcagattctg tgatgctcgt caggggggcg gagcctatgg aaaaacggca gcgccgcgtc
     6661 cttttctctt ccttgccttc acggcatatg tctctgcatt tctccgcgtg ccggttacgc
     6721 ccgccgcagc cgaacggcag gagcgtcagc gaccgagtga gcgaggaggc gtcatatctc
     6781 ctgcacaacc attatctgtc ttacgcccgg cagctgcatg tcatacctgc catatatagc
     6841 actttcgctg atacccatac caatgccaac atagtaagcc agtatacact ccgctagcgc
     6901 tacgtgactg gttcagggct gcgccccgaa acccgctaaa agccactgac gcgcctgcgg
     6961 cttgtccaac cccgcgccga ccgggaaagg ctttcccgtc cgtcacggcg ttatcaggag
     7021 gcgattctgg cagataaacg caacaagatg ctcacgatgt gggtgacgga agacgaacac
     7081 cggcggttgc ttgagcggtg cgacggaaaa caactggcgg cgtggatgcg ccagacctgc
     7141 cttgatgaga aaccgacacg cgccggcagg cttccctcga tttcgcccgc gctgctgcgt
     7201 cagcttgctg gcatgggcaa taacctgaac cagatcgccc ggaaagtgaa cgccggtggt
     7261 gccggtcatg accgggtgca gatcgtggca gcgctgatgg cgatcgatgc cggtctggaa
     7321 cggttgcggc atgccgtact ggaaaaaggg ccggacgatg attgttaaat tccatccccg
     7381 gggtcgcggc gggggcgccg gtcctgtgga ttatcttctg ggtaaagacc ggcagcgtga
     7441 aggtgccagc gtcctgcagg ggaagccgga agaagtccgg gagctgattg atgcgtcgcc
     7501 gtatgtgaaa aaatatacct cgggggtgct gtcattcgcg gaggcggacc tgccgccggg
     7561 tcagcgtgaa aaactgatgg ccagctttga gcgggttctg atgcccggac tcgataaaga
     7621 tcagtacagc attctgtggg tggaacacgc agacaagggt cgtcttgagc tgaattttct
     7681 gatccccaat accgaattac tgacggggaa acgcctccag ccgtattacg atcgtgccga
     7741 ccggccgcgc atcgatgcgt ggcagacagt ggtcaacggc aggctggggc tacatgatcc
     7801 gaacgacccg gagaaccggc gggcgctggt cacgccgtca ggactgccag aaacgaagca
     7861 ggaagccgca gagacgatta cacggggttt gctgatgtta gcctcatcag gagagctgaa
     7921 aacgcgtgat gacgtcacag gggcgctcac agcggccggt tttgaggtgg tcagaaccac
     7981 aaaaaacagt atcagcattg ccgacccgga cggggggcga aatatccgac tgaggggagc
     8041 catttatgag cagtctttta acggcctcga gggacttcga ggagaaatcg aaagcacagc
     8101 agcagcttac cgaagagagg ctgaaagccg cattcagcaa gcacgagaac gttgtcaaat
     8161 cggaactgaa cgcaagcgcg aagagaatca acgacgccat cagcgcccac gaacagggga
     8221 tgaacgcggc gatgcagtcg aaccggctga gcgtgctgcg aatggtcggg cggacgtggc
     8281 tgaccatcac catggtgtca gggctgctgt tcgccagcct gagcggggtg ctgtggtacc
     8341 aggggaaccg gatagcggcg aacctggcgg aaatcaggca gcagagagat acgctgtcga
     8401 aactgcagat gcagacgtgg ggcgtgacct atctggagaa ccgcaacgga cggtttctgg
     8461 tgctgccgga ggggatgaaa gccgaaacgg gccggacggt ggacaacaag acgcggaacg
     8521 cggtgaagct ggtacgggag tagagcagaa tgacggaact ggaaaaacaa ttgctgagcg
     8581 cgttagagca gctacagcag gactactcga gaaggctgga cgagtgggag agcgccttcg
     8641 aggaatggcg gaagatgtct ggtcttatgc aacgggagaa cgcggcgctg agcgagcgcg
     8701 tgtcgggctt gagcaggcag gtgcagagtt tgagcgagca gctgcgccgg ttatcgacag
     8761 gctgaatgag attgagcaac agcgatacgg gcaaagaacg gaaaaggaac cagagaagac
     8821 ag
//
