LOCUS       NZ_CP140477            15160 bp    DNA     circular CON 09-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain FF29-001BS plasmid
            Col440I, complete sequence.
ACCESSION   NZ_CP140477
VERSION     NZ_CP140477.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38451788
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 15160)
  AUTHORS   Manyahi,J.
  TITLE     bla NDM5 Klebsiella pneumoniae Tanzania
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15160)
  AUTHORS   Manyahi,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-NOV-2023) Clinical Science, University of Bergen,
            Haukelands veien 208, Bergen 5020, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method              :: SPAdes v. May-2022
            Genome Representation        :: Full
            Expected Final Version       :: Yes
            Genome Coverage              :: 12.0x
            Sequencing Technology        :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider          :: NCBI RefSeq
            Annotation Date              :: 01/08/2024 10:25:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision :: 6.6
            Features Annotated           :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                :: 18
            CDSs (total)                 :: 18
            Genes (coding)               :: 18
            CDSs (with protein)          :: 18
            Genes (RNA)                  :: 0
            tRNAs                        :: 0
            ncRNAs                       :: 0
            Pseudo Genes (total)         :: 0
            CDSs (without protein)       :: 0
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140477.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..15160
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FF29-001BS"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="Col440I"
                     /geo_loc_name="Tanzania: Dar es Salaam"
                     /lat_lon="6.8165 S 39.2894 E"
                     /collection_date="2022-03-03"
                     /collected_by="Joel Manyahi"
                     /genotype="ST437"
     gene            complement(join(15095..15160,1..126))
                     /locus_tag="SNM26_RS00005"
                     /old_locus_tag="SNM26_28530"
     CDS             complement(join(15095..15160,1..126))
                     /locus_tag="SNM26_RS00005"
                     /old_locus_tag="SNM26_28530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_055314556.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSLRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            536..652
                     /locus_tag="SNM26_RS00010"
                     /old_locus_tag="SNM26_28535"
     CDS             536..652
                     /locus_tag="SNM26_RS00010"
                     /old_locus_tag="SNM26_28535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_323811636.1"
                     /translation="MYARTPVQSDCCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1467..2303
                     /locus_tag="SNM26_RS00015"
                     /old_locus_tag="SNM26_28545"
     CDS             1467..2303
                     /locus_tag="SNM26_RS00015"
                     /old_locus_tag="SNM26_28545"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064106754.1"
                     /translation="MQKKYTIKLEQLRTDLDDYKKSRFVHSDKLMYDGINVNDRDRLIT
                     EIKNYIKDNDVINTETIINKLFPTKTPHLFISHKSEDAKEAITLANILHSKFGIESFID
                     SQVWHHINDIQKILDEEFSKKSENLYDYKKSNIVSSNIFSMLSSSLYRTIDDSDGFIYI
                     DRNDAHEDIGISKSNMVNIKTESPWIYLENIYTNSLRIKKHNRERIILEKSGLNNYAAD
                     SQVSIEERKEPKFIYSTNLVNAVKIKSLNNAFNVQYDKTKKHPLEGLDYFYNIILV"
                     /name="hypothetical protein"
     gene            complement(3274..3723)
                     /locus_tag="SNM26_RS00020"
                     /old_locus_tag="SNM26_28550"
     CDS             complement(3274..3723)
                     /locus_tag="SNM26_RS00020"
                     /old_locus_tag="SNM26_28550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_323811634.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKNPDPVMLQFSSPAIPGSS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3723..3914)
                     /locus_tag="SNM26_RS00025"
                     /old_locus_tag="SNM26_28555"
     CDS             complement(3723..3914)
                     /locus_tag="SNM26_RS00025"
                     /old_locus_tag="SNM26_28555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_055314556.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSLRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4324..4440
                     /locus_tag="SNM26_RS00030"
                     /old_locus_tag="SNM26_28560"
     CDS             4324..4440
                     /locus_tag="SNM26_RS00030"
                     /old_locus_tag="SNM26_28560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_323811636.1"
                     /translation="MYARTPVQSDCCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            5343..6089
                     /locus_tag="SNM26_RS00035"
                     /old_locus_tag="SNM26_28570"
     CDS             5343..6089
                     /locus_tag="SNM26_RS00035"
                     /old_locus_tag="SNM26_28570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_323811635.1"
                     /translation="MYDGINVNDRDRLITEIKNYIKDNDVINTETIINKLFPTKTPHLF
                     ISHKSEDAKEAITLANILHSKFGIESFIDSQVWHHINDIQKILDEEFSKKSENLYDYKK
                     SNIVSSNIFSMLSSSLYRTIDDSDGFIYIDRNDAHEDIGISKSNMVNIKTESPWIYLEN
                     IYTNSLRIKKHNRERIILEKSGLNNYAADSQVSIEERKEPKFIYSTNLVNAVKIKSLNN
                     AFNVQYDKTKKHPLEGLDYFYNIILV"
                     /name="hypothetical protein"
     gene            complement(7109..7510)
                     /locus_tag="SNM26_RS00040"
                     /old_locus_tag="SNM26_28575"
     CDS             complement(7109..7510)
                     /locus_tag="SNM26_RS00040"
                     /old_locus_tag="SNM26_28575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(7510..7701)
                     /locus_tag="SNM26_RS00045"
                     /old_locus_tag="SNM26_28580"
     CDS             complement(7510..7701)
                     /locus_tag="SNM26_RS00045"
                     /old_locus_tag="SNM26_28580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_055314556.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSLRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8113..8229
                     /locus_tag="SNM26_RS00050"
                     /old_locus_tag="SNM26_28585"
     CDS             8113..8229
                     /locus_tag="SNM26_RS00050"
                     /old_locus_tag="SNM26_28585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_323811636.1"
                     /translation="MYARTPVQSDCCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="replication initiation protein"
     gene            8564..9040
                     /locus_tag="SNM26_RS00055"
                     /old_locus_tag="SNM26_28590"
     CDS             8564..9040
                     /locus_tag="SNM26_RS00055"
                     /old_locus_tag="SNM26_28590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064106753.1"
                     /translation="MNDSKEAKIATLSNIQSIINRLADTSSKLKATYITIISAILTVFM
                     TYIIPKKEGIEGPFNPEDKLYSIIIFLVIFLAFLLIDSYYLRYERVMRKVYDSKARDIP
                     DDINSAINYFDISATYKKILKEGGVSYMPSKSQLLFYFIPFLVISITISMMVLR"
                     /name="hypothetical protein"
     gene            9045..9881
                     /locus_tag="SNM26_RS00060"
                     /old_locus_tag="SNM26_28595"
     CDS             9045..9881
                     /locus_tag="SNM26_RS00060"
                     /old_locus_tag="SNM26_28595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064106754.1"
                     /translation="MQKKYTIKLEQLRTDLDDYKKSRFVHSDKLMYDGINVNDRDRLIT
                     EIKNYIKDNDVINTETIINKLFPTKTPHLFISHKSEDAKEAITLANILHSKFGIESFID
                     SQVWHHINDIQKILDEEFSKKSENLYDYKKSNIVSSNIFSMLSSSLYRTIDDSDGFIYI
                     DRNDAHEDIGISKSNMVNIKTESPWIYLENIYTNSLRIKKHNRERIILEKSGLNNYAAD
                     SQVSIEERKEPKFIYSTNLVNAVKIKSLNNAFNVQYDKTKKHPLEGLDYFYNIILV"
                     /name="hypothetical protein"
     gene            complement(10901..11302)
                     /locus_tag="SNM26_RS00065"
                     /old_locus_tag="SNM26_28600"
     CDS             complement(10901..11302)
                     /locus_tag="SNM26_RS00065"
                     /old_locus_tag="SNM26_28600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(11302..11493)
                     /locus_tag="SNM26_RS00070"
                     /old_locus_tag="SNM26_28605"
     CDS             complement(11302..11493)
                     /locus_tag="SNM26_RS00070"
                     /old_locus_tag="SNM26_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_055314556.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSLRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            11909..12022
                     /locus_tag="SNM26_RS00075"
                     /old_locus_tag="SNM26_28610"
     CDS             11909..12022
                     /locus_tag="SNM26_RS00075"
                     /old_locus_tag="SNM26_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045907702.1"
                     /translation="MHEPPVQSDCCALSGNYQLESNPERHDKAPLAAATGN"
                     /name="hypothetical protein"
     gene            12357..12833
                     /locus_tag="SNM26_RS00080"
                     /old_locus_tag="SNM26_28615"
     CDS             12357..12833
                     /locus_tag="SNM26_RS00080"
                     /old_locus_tag="SNM26_28615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064106753.1"
                     /translation="MNDSKEAKIATLSNIQSIINRLADTSSKLKATYITIISAILTVFM
                     TYIIPKKEGIEGPFNPEDKLYSIIIFLVIFLAFLLIDSYYLRYERVMRKVYDSKARDIP
                     DDINSAINYFDISATYKKILKEGGVSYMPSKSQLLFYFIPFLVISITISMMVLR"
                     /name="hypothetical protein"
     gene            12838..13674
                     /locus_tag="SNM26_RS00085"
                     /old_locus_tag="SNM26_28620"
     CDS             12838..13674
                     /locus_tag="SNM26_RS00085"
                     /old_locus_tag="SNM26_28620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064106754.1"
                     /translation="MQKKYTIKLEQLRTDLDDYKKSRFVHSDKLMYDGINVNDRDRLIT
                     EIKNYIKDNDVINTETIINKLFPTKTPHLFISHKSEDAKEAITLANILHSKFGIESFID
                     SQVWHHINDIQKILDEEFSKKSENLYDYKKSNIVSSNIFSMLSSSLYRTIDDSDGFIYI
                     DRNDAHEDIGISKSNMVNIKTESPWIYLENIYTNSLRIKKHNRERIILEKSGLNNYAAD
                     SQVSIEERKEPKFIYSTNLVNAVKIKSLNNAFNVQYDKTKKHPLEGLDYFYNIILV"
                     /name="hypothetical protein"
     gene            complement(14694..15095)
                     /locus_tag="SNM26_RS00090"
                     /old_locus_tag="SNM26_28625"
     CDS             complement(14694..15095)
                     /locus_tag="SNM26_RS00090"
                     /old_locus_tag="SNM26_28625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_009310048.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQPKGEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     Typing          248..652
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.421"
                     /coverage="99.0"
                     /evalue="3.5900000000000006e-163"
     Typing          4036..4440
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.421"
                     /coverage="99.0"
                     /evalue="3.5900000000000006e-163"
     Typing          7823..8229
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.439"
                     /coverage="99.0"
                     /evalue="9.980000000000001e-164"
     Typing          11615..12022
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.154"
                     /coverage="99.0"
                     /evalue="2.76e-169"
ORIGIN
        1 gtggagtttc tcacaaaggt cggcctctac atcgaggtca agctcgttaa gcttctccag
       61 caggagcaga gactgattct ggatgaattt agccatgttc agcgctgttt tctgttgttt
      121 agtcataaga ggcctcagag cgtcagtgct cagtgtaagg gtgttctctt ttccgcttcc
      181 tcgctcactc agtcgctacg ctcctgccgt tcggctgcgg cgagcggcag gagcatgcgt
      241 agaaaagctc gagaaaaccg atcaatagcg tgatgaaaaa aaggaaaggc cgcgtggcgg
      301 ccctttttcc ataggcttcg cccccgacaa gcatcacaga aactgacgct caaatcagtg
      361 gtggcgaaac ccgacaggac tttaaagata ccaggcgttt ccccctgatg gctccctcgt
      421 gcgctctcct gttcctgcca ttcggtttgc cggtgtcatt ccgctgttat ggccgcgttt
      481 atctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
      541 tgcacgaacc cccgttcagt ccgactgctg cgccttgtcc ggtaactatc aacttgagtc
      601 caacccggaa agacacgaca aagcgccact ggcagcagcc actggtaact gatttagagg
      661 agttagtctt gaagttatgc gccagttgag gctaaactaa acgaacagtt ttggtgactg
      721 cgctcctcca agccagctac ctcggttcaa tcacctgcaa gtttttgaac cttcgaaaaa
      781 accgccctgc aaggcggttt tttccatttg cgagcctaag actacgactt caagacaagg
      841 atctcaagaa gatccttcct caactttaac actgctcggt ccaatcaaac accacattga
      901 ctcatactgt attaattact tctcgtattt ttttattacc tatggaataa tttagagata
      961 aacttatagc aaaaaaggag agtcttatga acgatagtaa agaagccaaa atcgcaacac
     1021 taagcaatat ccaatcaata attaacagac tagcagatac ctcttcaaag ctaaaagcaa
     1081 catacataac aatcatttca gcaattctta ctgtttttat gacatatata atacccaaaa
     1141 agaaggtata gaaggcccat tcaacccaga agacaaatta tatagcataa taatatttct
     1201 ggttattttt ttagcatttc ttctaataga ctcttactat cttagatatg aaagggttat
     1261 gcgaaaagtt tatgactcaa aggcgcgtga cattccagat gatataaaca gcgctataaa
     1321 ttactttgat attagcgcga cgtataaaaa aatcctaaaa gaaggtggcg ttagttacat
     1381 gccaagcaag tcacagcttt tattttattt tattcccttt cttgttatat ccataacaat
     1441 atccatgatg gtactgaggt gatgaaatgc aaaaaaaata cactataaaa cttgaacagt
     1501 tgcgtactga tttagatgat tacaaaaaaa gcagatttgt tcattcggac aaattaatgt
     1561 atgatggtat aaacgtcaac gatcgagaca ggttaataac cgaaataaaa aattacatca
     1621 aggataatga tgtaataaac acagaaacca tcattaataa actcttcccg acgaaaaccc
     1681 cgcacctttt catatcacac aaaagtgagg atgcgaaaga agcaataaca cttgcaaaca
     1741 tattacactc aaaatttggc atagaatcat ttattgactc tcaagtatgg catcacataa
     1801 acgacattca aaaaatactt gatgaagagt tttcaaaaaa aagcgaaaac ttatatgatt
     1861 acaaaaaaag caatatagta tccagcaata ttttctcaat gctttcatcc tcgctttaca
     1921 gaacgataga tgactcagat ggatttattt atatagatag aaatgacgca cacgaagaca
     1981 ttggcatatc gaaatcaaac atggttaata ttaaaacaga atcaccatgg atttatttgg
     2041 aaaacatata cacaaactca ttaagaataa aaaaacacaa tcgagaaaga atcattttag
     2101 aaaaatctgg gctgaacaac tacgcagcag attcacaagt atctatagaa gaaaggaaag
     2161 aaccaaaatt tatttactca acaaacctag taaatgcagt gaaaatcaaa agcctcaata
     2221 acgcatttaa cgttcaatat gacaagacaa aaaagcaccc cttagaaggc cttgattatt
     2281 tttacaatat catcttagta tagtattaac gcatagggtt atattttatt gccctatgcg
     2341 ttttcatctg taaaataata actccgcttt aataaagcag agaaagtcat cactccaaat
     2401 caaaaatcct tatggatatc tataacttca ctttctaact gagattcctg aatctgagga
     2461 ttatctaaaa gtcataatca caaataataa aaattacaga acaaatattc agaagtcagc
     2521 ctgtttcgcc actgagacca tttcaggcct tttgcgtctg tcatttaaca taaagccctg
     2581 attgtacgca ccgctaaaac ggcctcaggg cgtttctgac gggaaaccgg taaaacaggc
     2641 aacaaaccac ccgaaaaccc gccagaaacg cgcaggctct gtttttacgg catgcatgag
     2701 tatgcacgca agtgcatagc gcgaaaattc acgccattca gccagagtaa gtcctgtttc
     2761 cggcgttctt tccatccgaa aacggtgaac ggggacgcaa tttattgcgt ggccgtgaag
     2821 cgttgccggc gcagccggaa aaccggtttt cagcacgttc aggaatcggg ttcgtcgacg
     2881 gccggcaatc acgttatggc aagtcgccgg acgactcacc acacgattca ccggcgactc
     2941 ttcactagag tcgtacccag agtcgccgcg tctaaaaaac aaccaaacgg ggagtcgtgt
     3001 ctagagtcgg gtcgttatta acttattgtt tttatagtcc tgagtcgtta ccgactctat
     3061 gacgactcgc cggggactct atgacgactc gccggggact ctatgacgac tcgtcataga
     3121 gtcggatcta tataaataaa atttaaaaaa cgaaatttca ggaaagtaac ccactgatat
     3181 taaatacaaa tctgcaaaat gttaattttg cgtggggtgt gggtattttt agtggtgagg
     3241 agcgttcaga gatctgacaa tttttgggaa ggttcaggaa ctccccggga tggccggaga
     3301 ggaaaactga agcatgacgg ggtcaggatt tttccagaat ttccaccatg gcgccgggga
     3361 ttcagttaat tttgcctggt gattttcaag aaggagcatg gcctgtttca ggctgtcaat
     3421 gtgcgaatcc ttggacaaaa gtaaagtgtt caggtgttca atctcctgtt tcaaatgacg
     3481 aatcacctgt tcactctggt cagcagaggc tgtatcacgc tgttcagaga cctgtccaga
     3541 atggtgttca cccttcggtt gctcaaaggg accaaagaca cgcaaaagct ctgaagtatc
     3601 taccattttt gcattgcgtt caccgctaca ggtggacagg tcacctgctt taattaacct
     3661 gtgcagtgtt gttctactct tacccgtcag tcgggcagcc tcactaatag acactctggc
     3721 catcagttac catcctgtaa tgcatccagt ctcaaggaca gtgtacggaa aagatgctcc
     3781 gcatcatcgt ggagtttctc acaaaggtcg gcctctacat cgaggtcaag ctcgttaagc
     3841 ttctccagca ggagcagaga ctgattctgg atgaatttag ccatgttcag cgctgttttc
     3901 tgttgtttag tcataagagg cctcagagcg tcagtgctca gtgtaagggt gttctctttt
     3961 ccgcttcctc gctcactcag tcgctacgct cctgccgttc ggctgcggcg agcggcagga
     4021 gcatgcgtag aaaagctcga gaaaaccgat caatagcgtg atgaaaaaaa ggaaaggccg
     4081 cgtggcggcc ctttttccat aggcttcccc cctgacaagc atcacagaaa ctgacgctca
     4141 aatcagtggt ggcgaaaccc gacaggactt taaagatacc aggcgtttcc ccctgatggc
     4201 tccctcgtgc gctctcctgt tcctgccatt cggtttgccg gtgtcattcc gctgttatgg
     4261 ccgcgtttat ctcattccac gcctgacact cagttccggg taggcagttc gctccaagct
     4321 ggactgtatg cacgaacccc cgttcagtcc gactgctgcg ccttgtccgg taactatcaa
     4381 cttgagtcca acccggaaag acacgacaaa gcgccactgg cagcagccac tggtaactga
     4441 tttagaggag ttagtcttga agttatgcgc cagttgaggc taaactaaac gaacagtttt
     4501 ggtgactgcg ctcctccaag ccagctacct cggttcaatc acctgcaagt ttttgaacct
     4561 tcgaaaaaac cgccctgcaa ggcggttttt tccatttgcg agcctaagac tacgacttca
     4621 agacaaggat ctcaagaaga tccttcctca actttaacac tgctcggtcc aatcaaacac
     4681 cacattgact catactgtat taattacttc tcgtattttt ttattaccta tggaataatt
     4741 tagagataaa cttatagcaa aaaaggagag tcttatgaac gatagtaaag aagccaaaat
     4801 cgcaacacta agcaatatcc aatcaataat taacagacta gcagatacct cttcaaagct
     4861 aaaagcaaca tacataacaa tcatttcagc aattcttact gtttttatga catatataat
     4921 acccaaaaag aaggtataga aggcccattc aacccagaag acaaattata tagcataata
     4981 atatttctgg ttattttttt agcatttctt ctaatagact cttactatct tagatatgaa
     5041 agggttatgc gaaaagttta tgactcaaag gcgcgtgaca ttccagatga tataaacagc
     5101 gctataaatt actttgatat tagcgcgacg tataaaaaaa tcctaaaaga aggtggcgtt
     5161 agttacatgc caagcaagtc acagctttta ttttatttta ttccctttct tgttatatcc
     5221 ataacaatat ccatgatggt actgaggtga tgaaatgcaa aaaaaataca ctataaaact
     5281 tgaacagttg cgtactgatt tagatgatca aaaaaagcag atttgttcat tcggacaaat
     5341 taatgtatga tggtataaac gtcaacgatc gagacaggtt aataaccgaa ataaaaaatt
     5401 acatcaagga taatgatgta ataaacacag aaaccatcat taataaactc ttcccgacga
     5461 aaaccccgca ccttttcata tcacacaaaa gtgaggatgc gaaagaagca ataacacttg
     5521 caaacatatt acactcaaaa tttggcatag aatcatttat tgactctcaa gtatggcatc
     5581 acataaacga cattcaaaaa atacttgatg aagagttttc aaaaaaaagc gaaaacttat
     5641 atgattacaa aaaaagcaat atagtatcca gcaatatttt ctcaatgctt tcatcctcgc
     5701 tttacagaac gatagatgac tcagatggat ttatttatat agatagaaat gacgcacacg
     5761 aagacattgg catatcgaaa tcaaacatgg ttaatattaa aacagaatca ccatggattt
     5821 atttggaaaa catatacaca aactcattaa gaataaaaaa acacaatcga gaaagaatca
     5881 ttttagaaaa atctgggctg aacaactacg cagcagattc acaagtatct atagaagaaa
     5941 ggaaagaacc aaaatttatt tactcaacaa acctagtaaa tgcagtgaaa atcaaaagcc
     6001 tcaataacgc atttaacgtt caatatgaca agacaaaaaa gcacccctta gaaggccttg
     6061 attattttta caatatcatc ttagtatagt attaacgcat agggttatat tttattgccc
     6121 tatgcgtttt catctgtaaa ataataactc cgctttaata aagcagagaa agtcatcact
     6181 ccaaatcaaa aatccttatg gatatctata acttcacttt ctaactgaga ttcctgaatc
     6241 tgaggattat ctaaaagtca taatcacaaa taataaaaat tacagaacaa atattcagaa
     6301 gtcagcctgt ttcgccactg agaccatttc aggccttttg cgtctgtcat ttaacataaa
     6361 gccctgattg tacgcaccgc taaaacggcc tcagggcgtt tctgacggga aaccggtaaa
     6421 acaggcaaca aaccacccga aaacccgcca gaaacgcgca ggctctgttt ttacggcatg
     6481 catgagtatg cacgcaagtg catagcgcga aaattcacgc cattcagcca gagtaagtcc
     6541 tgtttccggc gttctttcca tccgaaaacg gtgaacgggg acgcaattta ttgcgtggcc
     6601 gtgaagcgtt gccggcgcag ccggaaaacc ggttttcagc acgttcagga atcgggttcg
     6661 tcgacggccg gcaatcacgt tatggcaagt cgccggacga ctcaccacac gattcaccgg
     6721 cgactcttca ctagagtcgt acccagagtc gccgcgtcta aaaaacaacc aaacggggag
     6781 tcgtgtctag agtcgggtcg ttattaactt attgttttta tagtcctgag tcgttaccga
     6841 ctctatgacg actcgccggg gactctatga cgactcgccg gggactctat gacgactcgt
     6901 catagagtcg gatctatata aataaaattt aaaaaacgaa atttcaggaa agtaacccac
     6961 tgatattaaa tacaaatctg caaaatgtta attttgcgtg gggtgtgggt atttttagtg
     7021 gtgaggagcg ttcagagatc tgacaatttt tgggaaggtt caggaactcc ccgggatggc
     7081 cggagaggaa aactgaagca tgacggggtc aggatttttt ccagaatttc caccatggcg
     7141 ccggggattc agttaatttt gcctggtgat tttcaagaag gagcatggcc tgtttcaggc
     7201 tgtcaatgtg cgaatccttg gacaaaagta aagtgttcag gtgttcaatc tcctgtttca
     7261 aatgacgaat cacctgttca ctctggtcag cagaggctgt atcacgctgt tcagagacct
     7321 gtccagaatg gtgttcaccc ttcggttgct caaagggacc aaagacacgc aaaagctctg
     7381 aagtatctac catttttgca ttgcgttcac cgctacaggt ggacaggtca cctgctttaa
     7441 ttaacctgtg cagtgttgtt ctactcttac ccgtcagtcg ggcagcctca ctaatagaca
     7501 ctctggccat cagttaccat cctgtaatgc atccagtctc aaggacagtg tacggaaaag
     7561 atgctccgca tcatcgtgga gtttctcaca aaggtcggcc tctacatcga ggtcaagctc
     7621 gttaagcttc tccagcagga gcagagactg attctggatg aatttagcca tgttcagcgc
     7681 tgttttctgt tgtttagtca taagaggcct cagagcgtca gtgctcagtg taagggtgtt
     7741 ctcttttccg cttcctcgct cactcagtcg ctacgctcct gccgttcggc tgcggcgagc
     7801 ggcaggagca tgcgtagaaa agctcgagaa aaccgatcaa tagcgtgatg aaaaaaagga
     7861 aaggccgcgt ggcggccctt tttccatagg cttcgccccc ctgacaagca tcacagaaac
     7921 tgacgctcaa atcagtggtg gcgaaacccg acaggacttt aaagatacca ggcgttttcc
     7981 ccctgtggct ccctcgtgcg ctctcctgtt cctgccattc ggtttgccgg tgtcattccg
     8041 ctgttatggc cgcgtttatc tcattccacg cctgacactc agttccgggt aggcagttcg
     8101 ctccaagctg gactgtatgc acgaaccccc gttcagtccg actgctgcgc cttgtccggt
     8161 aactatcaac ttgagtccaa cccggaaaga cacgacaaag cgccactggc agcagccact
     8221 ggtaactgat ttagaggagt tagtcttgaa gttatgcgcc agttgaggct aaactaaacg
     8281 aacagttttg gtgactgcgc tcctccaagc cagctacctc ggttcaatca cctgcaagtt
     8341 tttgaacctt cgaaaaaacc gccctgcaag gcggtttttt ccatttgcga gcctaagact
     8401 acgacttcaa gacaaggatc tcaagaagat ccttcctcaa ctttaacact gctcggtcca
     8461 atcaaacacc acattgactc atactgtatt aattacttct cgtatttttt tattacctat
     8521 ggaataattt agagataaac ttatagcaaa aaaggagagt cttatgaacg atagtaaaga
     8581 agccaaaatc gcaacactaa gcaatatcca atcaataatt aacagactag cagatacctc
     8641 ttcaaagcta aaagcaacat acataacaat catttcagca attcttactg tttttatgac
     8701 atatataata cccaaaaaag aaggtataga aggcccattc aacccagaag acaaattata
     8761 tagcataata atatttctgg ttattttttt agcatttctt ctaatagact cttactatct
     8821 tagatatgaa agggttatgc gaaaagttta tgactcaaag gcgcgtgaca ttccagatga
     8881 tataaacagc gctataaatt actttgatat tagcgcgacg tataaaaaaa tcctaaaaga
     8941 aggtggcgtt agttacatgc caagcaagtc acagctttta ttttatttta ttccctttct
     9001 tgttatatcc ataacaatat ccatgatggt actgaggtga tgaaatgcaa aaaaaataca
     9061 ctataaaact tgaacagttg cgtactgatt tagatgatta caaaaaaagc agatttgttc
     9121 attcggacaa attaatgtat gatggtataa acgtcaacga tcgagacagg ttaataaccg
     9181 aaataaaaaa ttacatcaag gataatgatg taataaacac agaaaccatc attaataaac
     9241 tcttcccgac gaaaaccccg caccttttca tatcacacaa aagtgaggat gcgaaagaag
     9301 caataacact tgcaaacata ttacactcaa aatttggcat agaatcattt attgactctc
     9361 aagtatggca tcacataaac gacattcaaa aaatacttga tgaagagttt tcaaaaaaaa
     9421 gcgaaaactt atatgattac aaaaaaagca atatagtatc cagcaatatt ttctcaatgc
     9481 tttcatcctc gctttacaga acgatagatg actcagatgg atttatttat atagatagaa
     9541 atgacgcaca cgaagacatt ggcatatcga aatcaaacat ggttaatatt aaaacagaat
     9601 caccatggat ttatttggaa aacatataca caaactcatt aagaataaaa aaacacaatc
     9661 gagaaagaat cattttagaa aaatctgggc tgaacaacta cgcagcagat tcacaagtat
     9721 ctatagaaga aaggaaagaa ccaaaattta tttactcaac aaacctagta aatgcagtga
     9781 aaatcaaaag cctcaataac gcatttaacg ttcaatatga caagacaaaa aagcacccct
     9841 tagaaggcct tgattatttt tacaatatca tcttagtata gtattaacgc atagggttat
     9901 attttattgc cctatgcgtt ttcatctgta aaataataac tccgctttaa taaagcagag
     9961 aaagtcatca ctccaaatca aaaatcctta tggatatcta taacttcact ttctaactga
    10021 gattcctgaa tctgaggatt atctaaaagt cataatcaca aataataaaa attacagaac
    10081 aaatattcag aagtcagcct gtttcgccac tgagaccatt tcaggccttt tgcgtctgtc
    10141 atttaacata aagccctgat tgtacgcacc gctaaaacgg cctcagggcg tttctgacgg
    10201 gaaaccggta aaacaggcaa caaaccaccc gaaaacccgc cagaaacgcg caggctctgt
    10261 ttttacggca tgcatgagta tgcacgcaag tgcatagcgc gaaaattcac gccattcagc
    10321 cagagtaagt cctgtttccg gcgttctttc catccgaaaa cggtgaacgg ggacgcaatt
    10381 tattgcgtgg ccgtgaagcg ttgccggcgc agccggaaaa ccggttttca gcacgttcag
    10441 gaatcgggtt cgtcgacggc cggcaatcac gttatggcaa gtcgccggac gactcaccac
    10501 acgattcacc ggcgactctt cactagagtc gtacccagag tcgccgcgtc taaaaaacaa
    10561 ccaaacgggg agtcgtgtct agagtcgggt cgttattaac ttattgtttt tatagtcctg
    10621 agtcgttacc gactctatga cgactcgccg gggactctat gacgactcgc cggggactct
    10681 atgacgactc gtcatagagt cggatctata taaataaaat ttaaaaaacg aaatttcagg
    10741 aaagtaaccc actgatatta aatacaaatc tgcaaaatgt taattttgcg tggggtgtgg
    10801 gtatttttag tggtgaggag cgttcagaga tctgacaatt tttgggaagg ttcaggaact
    10861 ccccgggatg gccggagagg aaaactgaag catgacgggg tcaggatttt ttccagaatt
    10921 tccaccatgg cgccggggat tcagttaatt ttgcctggtg attttcaaga aggagcatgg
    10981 cctgtttcag gctgtcaatg tgcgaatcct tggacaaaag taaagtgttc aggtgttcaa
    11041 tctcctgttt caaatgacga atcacctgtt cactctggtc agcagaggct gtatcacgct
    11101 gttcagagac ctgtccagaa tggtgttcac ccttcggttg ctcaaaggga ccaaagacac
    11161 gcaaaagctc tgaagtatct accatttttg cattgcgttc accgctacag gtggacaggt
    11221 cacctgcttt aattaacctg tgcagtgttg ttctactctt acccgtcagt cgggcagcct
    11281 cactaataga cactctggcc atcagttacc atcctgtaat gcatccagtc tcaaggacag
    11341 tgtacggaaa agatgctccg catcatcgtg gagtttctca caaaggtcgg cctctacatc
    11401 gaggtcaagc tcgttaagct tctccagcag gagcagagac tgattctgga tgaatttagc
    11461 catgttcagc gctgttttct gttgtttagt cataagaggc ctcagagcgt cagtgctcag
    11521 tgtaagggtg ttctcttttc cgcttcctcg ctcactcagt cgctacgctc ctgccgttcg
    11581 gctgcggcga gcggcaggag catgcgtaga aaagctcgag aaaaccgatc aatagcgtga
    11641 tgaaaaaaag gaaaggccgc gtggcggccc tttttccata ggcttcgccc ccctgacaag
    11701 catcacagaa actgacgctc aaatcagtgg tggcgaaacc cgacaggact ttaaagatac
    11761 caggcgtttc cccctgatgg ctccctcgtg cgctctcctg ttcctgccat tcggtttgcc
    11821 ggtgtcattc cgctgttatg gccgcgttta tctcattcca cgcctgacac tcagttccgg
    11881 gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt ccgactgctg
    11941 cgccttgtcc ggtaactatc aacttgagtc caacccggaa agacacgaca aagcgccact
    12001 ggcagcagcc actggtaact gatttagagg agttagtctt gaagttatgc gccagttgag
    12061 gctaaactaa acgaacagtt ttggtgactg cgctcctcca agccagctac ctcggttcaa
    12121 tcacctgcaa gtttttgaac cttcgaaaaa accgccctgc aaggcggttt tttccatttg
    12181 cgagcctaag actacgactt caagacaagg atctcaagaa gatccttcct caactttaac
    12241 actgctcggt ccaatcaaac accacattga ctcatactgt attaattact tctcgtattt
    12301 ttttattacc tatggaataa tttagagata aacttatagc aaaaaaggag agtcttatga
    12361 acgatagtaa agaagccaaa atcgcaacac taagcaatat ccaatcaata attaacagac
    12421 tagcagatac ctcttcaaag ctaaaagcaa catacataac aatcatttca gcaattctta
    12481 ctgtttttat gacatatata atacccaaaa aagaaggtat agaaggccca ttcaacccag
    12541 aagacaaatt atatagcata ataatatttc tggttatttt tttagcattt cttctaatag
    12601 actcttacta tcttagatat gaaagggtta tgcgaaaagt ttatgactca aaggcgcgtg
    12661 acattccaga tgatataaac agcgctataa attactttga tattagcgcg acgtataaaa
    12721 aaatcctaaa agaaggtggc gttagttaca tgccaagcaa gtcacagctt ttattttatt
    12781 ttattccctt tcttgttata tccataacaa tatccatgat ggtactgagg tgatgaaatg
    12841 caaaaaaaat acactataaa acttgaacag ttgcgtactg atttagatga ttacaaaaaa
    12901 agcagatttg ttcattcgga caaattaatg tatgatggta taaacgtcaa cgatcgagac
    12961 aggttaataa ccgaaataaa aaattacatc aaggataatg atgtaataaa cacagaaacc
    13021 atcattaata aactcttccc gacgaaaacc ccgcaccttt tcatatcaca caaaagtgag
    13081 gatgcgaaag aagcaataac acttgcaaac atattacact caaaatttgg catagaatca
    13141 tttattgact ctcaagtatg gcatcacata aacgacattc aaaaaatact tgatgaagag
    13201 ttttcaaaaa aaagcgaaaa cttatatgat tacaaaaaaa gcaatatagt atccagcaat
    13261 attttctcaa tgctttcatc ctcgctttac agaacgatag atgactcaga tggatttatt
    13321 tatatagata gaaatgacgc acacgaagac attggcatat cgaaatcaaa catggttaat
    13381 attaaaacag aatcaccatg gatttatttg gaaaacatat acacaaactc attaagaata
    13441 aaaaaacaca atcgagaaag aatcatttta gaaaaatctg ggctgaacaa ctacgcagca
    13501 gattcacaag tatctataga agaaaggaaa gaaccaaaat ttatttactc aacaaaccta
    13561 gtaaatgcag tgaaaatcaa aagcctcaat aacgcattta acgttcaata tgacaagaca
    13621 aaaaagcacc ccttagaagg ccttgattat ttttacaata tcatcttagt atagtattaa
    13681 cgcatagggt tatattttat tgccctatgc gttttcatct gtaaaataat aactccgctt
    13741 taataaagca gagaaagtca tcactccaaa tcaaaaatcc ttatggatat ctataacttc
    13801 actttctaac tgagattcct gaatctgagg attatctaaa agtcataatc acaaataata
    13861 aaaattacag aacaaatatt cagaagtcag cctgtttcgc cactgagacc atttcaggcc
    13921 ttttgcgtct gtcatttaac ataaagccct gattgtacgc accgctaaaa cggcctcagg
    13981 gcgtttctga cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac
    14041 gcgcaggctc tgtttttacg gcatgcatga gtatgcacgc aagtgcatag cgcgaaaatt
    14101 cacgccattc agccagagta agtcctgttt ccggcgttct ttccatccga aaacggtgaa
    14161 cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaaccggttt
    14221 tcagcacgtt caggaatcgg gttcgtcgac ggccggcaat cacgttatgg caagtcgccg
    14281 gacgactcac cacacgattc accggcgact cttcactaga gtcgtaccca gagtcgccgc
    14341 gtctaaaaaa caaccaaacg gggagtcgtg tctagagtcg ggtcgttatt aacttattgt
    14401 ttttatagtc ctgagtcgtt accgactcta tgacgactcg ccggggactc tatgacgact
    14461 cgccggggac tctatgacga ctcgtcatag agtcggatct atataaataa aatttaaaaa
    14521 acgaaatttc aggaaagtaa cccactgata ttaaatacaa atctgcaaaa tgttaatttt
    14581 gcgtggggtg tgggtatttt tagtggtgag gagcgttcag agatctgaca atttttggga
    14641 aggttcagga actccccggg atggccggag aggaaaactg aagcatgacg gggtcaggat
    14701 tttttccaga atttccacca tggcgccggg gattcagtta attttgcctg gtgattttca
    14761 agaaggagca tggcctgttt caggctgtca atgtgcgaat ccttggacaa aagtaaagtg
    14821 ttcaggtgtt caatctcctg tttcaaatga cgaatcacct gttcactctg gtcagcagag
    14881 gctgtatcac gctgttcaga gacctgtcca gaatggtgtt cacccttcgg ttgctcaaag
    14941 ggaccaaaga cacgcaaaag ctctgaagta tctaccattt ttgcattgcg ttcaccgcta
    15001 caggtggaca ggtcacctgc tttaattaac ctgtgcagtg ttgttctact cttacccgtc
    15061 agtcgggcag cctcactaat agacactctg gccatcagtt accatcctgt aatgcatcca
    15121 gtctcaagga cagtgtacgg aaaagatgct ccgcatcatc
//
