LOCUS       NZ_CP140424             3853 bp    DNA     circular CON 22-DEC-2023
DEFINITION  Acinetobacter baumannii strain 2023CK-00893 plasmid unnamed4,
            complete sequence.
ACCESSION   NZ_CP140424
VERSION     NZ_CP140424.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN36514289
            Assembly: GCF_034494405.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Pathogen: clinical or host-associated sample
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Hergert,J., Casey,R., Wagner,J., Young,E.L. and Oakeson,K.F.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-DEC-2023) Utah Public Health Laboratory, Utah Public
            Health Laboratory Infectious Disease submission group, 4431 S 2700
            W, Salt Lake City, UT 84129, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 70.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/22/2023 14:24:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,146
            CDSs (total)                      :: 4,049
            Genes (coding)                    :: 3,947
            CDSs (with protein)               :: 3,947
            Genes (RNA)                       :: 97
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 75
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 102
            CDSs (without protein)            :: 102
            Pseudo Genes (ambiguous residues) :: 0 of 102
            Pseudo Genes (frameshifted)       :: 45 of 102
            Pseudo Genes (incomplete)         :: 47 of 102
            Pseudo Genes (internal stop)      :: 23 of 102
            Pseudo Genes (multiple problems)  :: 10 of 102
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140424.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="2023CK-00893"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="unnamed4"
                     /geo_loc_name="USA"
                     /collection_date="2023-06-18"
     gene            complement(join(2405..3853,1..453))
                     /locus_tag="U2S75_RS20720"
                     /old_locus_tag="U2S75_20715"
     CDS             complement(join(2405..3853,1..453))
                     /locus_tag="U2S75_RS20720"
                     /old_locus_tag="U2S75_20715"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_322546972.1"
                     /translation="MIVQFFRYGNGLSKGPLDYLLGKDRDRDYAKVLQGDVSEVSGLID
                     TSPYAKKYTSGCLSFYEHDLSDQDKQKIMQDFEQALFPGMDQSQYRVLWIEHQDKLNEE
                     TGERRLELNFLIPNVEILTGQRLQPYYDKADRPRIDLFKKITNYEYQLHDADDPLYRQA
                     VTTAKNLPKSVNEIKETIDIEATRAVESGLITDRQSMKKWLSDLGLEITRETKKSLSIK
                     NPNGGENSRNISLKGVIYEQDFRLTETSQQLTIAASERYRREAEQRNQSDIQRYAEYSA
                     KRSTELEQQYRQHQNGYTAETERSNERDIAADRPDYSSNQEAAATRHDSPIARTTTADQ
                     TAKSELAAVERLEPRNSPSSQTKENPYYIEYSLDFTGMYNAYQQYLSGIRQQEQVQRHH
                     RDGEQSPVAKKPRGEPEYSEVWDREKMGLVRPDRPESAAVQQQYANSAGNQLNESRSTV
                     IADYRAATAAAQRATEAARASLAAHTGTEQNHRRTRELLQQIDNSLQHADRERKRDEKS
                     YQTATESEAIRDFITKLGEQLKTAITEPFRAISDWIKSKERDQRTIANDDLYRNREADS
                     TIDRTASKETRLSATVSRTIRGFDQTNIFKALDELDRRRELQLEQQRKNDRSYSPRF"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(450..896)
                     /locus_tag="U2S75_RS20725"
                     /old_locus_tag="U2S75_20720"
     CDS             complement(450..896)
                     /locus_tag="U2S75_RS20725"
                     /old_locus_tag="U2S75_20720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_322546973.1"
                     /translation="MERKDTWRPLCYADVFHDLVQGQAPETPKSKGKSMTAIKRNKVYK
                     VRFSDAEVQKIKDANILNIAKYLRDTSLAQIDVTQGNAKKKNQENHYTKLDRDFLLELS
                     RIGGNINQIAKAINTDIASSRPLDAARLLHLLIGIDQTLKELKK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1155..2117)
                     /locus_tag="U2S75_RS20730"
                     /old_locus_tag="U2S75_20725"
     CDS             complement(1155..2117)
                     /locus_tag="U2S75_RS20730"
                     /old_locus_tag="U2S75_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004918192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_322546974.1"
                     /translation="MLNKTQFSDDFYEKLPKKPYCSDDLGRGVIIRPKRTAIQKPYIQH
                     NPPCLVSSLVFDIDRQDAYFAWSDANLPTPTWIAKNRQNGHAHIGYMLLAPVCTTHRAK
                     QNVIQYLAKIEQAYSLALGADRGYTGLITKNPCHGTWENHTFGVQPYDLNYLADFVELQ
                     ELKTDLKEVSGLGRNCMMFDTVRFWAYEAIRAFISGSPDSWYAEVLKVAINANGAFLEP
                     LPHSEVKATAKSVARWVWRNHTAAHAKFIERQTAKGRKGGLSSDSSHGGKARSAQYSDI
                     RQEALKLHIMGLSVTKIANQLGISRPSVYAYLKSVKVQA"
                     /name="replication initiation protein"
     Typing          complement(2412..3853)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.739"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttgatattca tagtttgtaa tcttcttaaa cagatcaata cgaggacgat cagctttatc
       61 ataataaggc tgtaaacgtt gtcctgtaag gatttcaaca ttagggatta ggaagttaag
      121 ctctaatctt cgctcccctg tttcttcgtt taatttgtct tgatgctcaa tccacaagac
      181 acggtactgg ctctgatcca taccagggaa taaggcttgc tcaaaatcct gcatgatttt
      241 ttgtttatcc tggtcagata aatcatgctc ataaaaactt aggcatccgg atgtatattt
      301 ttttgcataa ggtgacgtat caattaatcc tgatacttcg gaaacatcgc cctgcaaaac
      361 ctttgcatag tcacgatcac ggtctttacc aagtaaataa tccagtggac ctttgctcaa
      421 accattgccg tatctaaaga actgaacaat cattttttca attcctttaa ggtctgatca
      481 atccctatta acaggtgcaa aagccttgca gcatcaaggg gacgactgct tgcaatatcg
      541 gtatttattg cctttgcaat ctgattgatg ttgcctccaa ttcgggatag ctcaagcaag
      601 aagtcacggt ctaattttgt gtagtggttt tcctgattct tttttttggc gttgccttga
      661 gttacatcaa tttgggcaag cgaagtatcc cttagatatt tggcaatatt taggatattg
      721 gcatctttga ttttctgtac ctctgcatca gaaaatcgga ctttgtaaac cttattacgt
      781 ttgatcgctg tcatgctttt gcctttgctt ttgggggttt cgggggcttg cccctgaaca
      841 aggtcatgga acacatctgc gtagcagagg ggtctccatg tatccttgcg ttccattacg
      901 atagcagacc cctagacgta attcaattaa gcaggtcatg gcaagactat ggacaatcgc
      961 aagcgaccgt aaaacctgat ctgtgcctaa acactatgca caactccgca taacgataaa
     1021 gccgttatac gttgagttgc acacaggttt agatcacagg gttttacttt gcccacagcc
     1081 gttactcatt cccttaaata ataattttta aaaatggaat gggagaaaaa caccctagag
     1141 ggctgatatc tgatttaggc ttgcactttt acacttttta gataggcata aacactaggt
     1201 cgagatattc ctagttgatt ggctattttc gtcacactaa gccccataat atgcagtttt
     1261 aaggcttcct gacgtatatc gctgtactgt gctgatctag ccttaccgcc atgactggaa
     1321 tcactgctta aaccgccttt acgcccttta gcagtttgac gctcgataaa tttggcgtgt
     1381 gctgctgtat ggttacgcca tacccaacgt gccactgatt tagctgtcgc tttaacctct
     1441 gaatgtggca atggctctaa aaacgcacca tttgcgttta tagcgacttt tagcacttcc
     1501 gcataccaac tatcaggcga ccccgaaata aacgctctaa tcgcttcata cgcccaaaat
     1561 ctaacggtat cgaacatcat acagttacgc ccaagccctg aaacctcttt taagtcggtt
     1621 tttagttcct gaagctctac aaaatcggct aaataattga ggtcgtatgg ttgaacgcca
     1681 aaggtatgat tttcccatgt tccgtgacat ggatttttag tgattaagcc cgtatagcca
     1741 cgatctgcac ctaacgccaa gctatacgcc tgctcaatct tggcaaggta ctgtatgaca
     1801 ttttgctttg ctcgatgcgt ggtacacact ggagctaaaa gcatatagcc aatatgtgca
     1861 tgtccatttt ggcgattttt ggctatccaa gtcggtgtag gtaggtttgc atcagaccac
     1921 gcaaaataag cgtcttggcg atctatatcg aacaccaatg agctaacaag acaaggggga
     1981 ttatgctgga tgtatggctt ctggattgcg gtacgttttg ggcgaataat caccccacgc
     2041 cctaaatcat ccgagcaata cggttttttt gggagttttt cgtaaaaatc gtctgaaaat
     2101 tgcgttttat tgagcatgaa acacgtccaa gtgttgcaat agctcaaaac atcagtaaaa
     2161 tacaagtatc gaatttgcgt tttaccgatg ctttaagcgt ttgtttaaca aagagaaaaa
     2221 agtaggtttg cagacctgct tttttttcgc ctgatgaaaa tattacacct ttttgaatta
     2281 ctgtcaaatg actgattttc aaaacaaaat attgcgacaa attgtcgcaa atatgcgccc
     2341 tttggagatg cttttttttc gaggacagca ttgcaagggc aatgctccgc agacaaaaaa
     2401 agtatcaaaa tctaggtgaa taactcctgt catttttacg ttgttgctcc agttgcaact
     2461 ctctacgtct gtccaactca tctaaagcct tgaagatatt tgtttgatca aaccctcgaa
     2521 tcgttcgtga tactgttgca ctgagtctag tttcttttga agctgttcgg tcaatggtgc
     2581 tgtctgcttc tcgatttctg tatagatcat cgttggcaat agttcgttga tctcgttctt
     2641 tagatttaat ccagtcacta attgctctaa atggctctgt aatcgctgtt ttaagctgct
     2701 ctccaagttt tgtaataaag tctctgatag cttcggattc tgtagctgtc tgatatgatt
     2761 tttcatctct ttttctttct cggtcagcgt gttgaagtga gttatcaatt tgttgcagta
     2821 gctctctagt tcgtctatgg ttctgttctg tgcctgtatg agctgcaagg cttgctcttg
     2881 ctgcttctgt tgctctttga gctgctgctg ttgctgctcg ataatctgca ataactgtgc
     2941 ttcgagattc atttaattgg tttcctgcac tattcgcata ttgttgctgt actgctgcgc
     3001 tttcgggtcg atctggacgc accagtccca ttttttctct gtcccacact tctgaatatt
     3061 caggctcgcc ccttggcttt ttagccactg gactttgttc tccatctctg tgatgtcgct
     3121 gtacttgctc ttgctgacgt atcccagaca aatactgctg ataagcgtta tacatgccag
     3181 tgaaatcaag gctatattcg atgtaataag ggttttcttt agtttggctg cttgggctgt
     3241 ttcttggctc aagtcgttca actgctgcaa gttcgctttt agctgtttga tctgctgttg
     3301 ttgttcttgc aatagggctg tcatgccttg ttgctgctgc ttcttggttt gagctgtagt
     3361 ctggtcgatc tgctgcaata tctctttcgt tgcttcgctc tgtttctgct gtatatccgt
     3421 tttggtgctg tcgatactgc tgctcaagct ctgtgcttcg ctttgcactg tattccgcat
     3481 atctttgaat atctgactga tttcgctgtt cagcttctcg tctatatcgt tctgatgctg
     3541 cgatagtaag ttgttggctc gtttctgtaa gtctaaaatc ttgctcatag atcacacctt
     3601 taaggcttat atttcgggag ttctctccac catttggatt tttaatggat aggctttttt
     3661 tggtttctct ggtgatttca agaccaagat ccgatagcca ttttttcatg ctctgtcgat
     3721 ctgtaattaa tccactctca acggctctcg ttgcttcaat atcaatggtt tccttaattt
     3781 cattaacaga cttcggcaag ttttttgccg ttgtaacggc ttggcgatag agggggtcat
     3841 ctgcatcatg cag
//
