LOCUS       NZ_CP140296            12282 bp    DNA     circular CON 22-DEC-2023
DEFINITION  Klebsiella pneumoniae strain KP33_NE2_41.22_ST2096_OXA232 plasmid
            pKP33_OXA232, complete sequence.
ACCESSION   NZ_CP140296
VERSION     NZ_CP140296.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38792442
            Assembly: GCF_034479915.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12282)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     q20 nanopore sequence analysis of isolates of Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12282)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: FEB-2023
            Assembly Method :: flye v. 2.9.1-b1780; medaka v. 1.7.2
            Assembly Name                     :: rrp8_bc09
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 116x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/22/2023 14:26:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,675
            CDSs (total)                      :: 5,554
            Genes (coding)                    :: 5,375
            CDSs (with protein)               :: 5,375
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 179
            CDSs (without protein)            :: 179
            Pseudo Genes (ambiguous residues) :: 0 of 179
            Pseudo Genes (frameshifted)       :: 89 of 179
            Pseudo Genes (incomplete)         :: 92 of 179
            Pseudo Genes (internal stop)      :: 27 of 179
            Pseudo Genes (multiple problems)  :: 26 of 179
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP140296.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12282
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP33_NE2_41.22_ST2096_OXA232"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP33_OXA232"
                     /geo_loc_name="United Kingdom: England"
                     /collection_date="2022-10-08"
                     /genotype="ST2096"
     gene            complement(join(11726..12282,1..241))
                     /locus_tag="U3T26_RS28310"
                     /old_locus_tag="U3T26_28310"
     CDS             complement(join(11726..12282,1..241))
                     /locus_tag="U3T26_RS28310"
                     /old_locus_tag="U3T26_28310"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            585..866
                     /locus_tag="U3T26_RS28315"
                     /old_locus_tag="U3T26_28315"
     CDS             585..866
                     /locus_tag="U3T26_RS28315"
                     /old_locus_tag="U3T26_28315"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(1148..2602)
                     /locus_tag="U3T26_RS28320"
                     /old_locus_tag="U3T26_28320"
     CDS             complement(1148..2602)
                     /locus_tag="U3T26_RS28320"
                     /old_locus_tag="U3T26_28320"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2583..2921)
                     /locus_tag="U3T26_RS28325"
                     /old_locus_tag="U3T26_28325"
     CDS             complement(2583..2921)
                     /locus_tag="U3T26_RS28325"
                     /old_locus_tag="U3T26_28325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2903..3295)
                     /locus_tag="U3T26_RS28330"
                     /old_locus_tag="U3T26_28330"
     CDS             complement(2903..3295)
                     /locus_tag="U3T26_RS28330"
                     /old_locus_tag="U3T26_28330"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(3703..4650)
                     /locus_tag="U3T26_RS28335"
                     /old_locus_tag="U3T26_28335"
     CDS             complement(3703..4650)
                     /locus_tag="U3T26_RS28335"
                     /old_locus_tag="U3T26_28335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            <5026..5178
                     /locus_tag="U3T26_RS28340"
                     /old_locus_tag="U3T26_28340"
                     /pseudo=""
     CDS             <5026..5178
                     /locus_tag="U3T26_RS28340"
                     /old_locus_tag="U3T26_28340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     gene            complement(5585..6382)
                     /locus_tag="U3T26_RS28345"
                     /old_locus_tag="U3T26_28345"
     CDS             complement(5585..6382)
                     /locus_tag="U3T26_RS28345"
                     /old_locus_tag="U3T26_28345"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011071128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /protein_id="WP_043907054.1"
                     /translation="MRVLALSAVFLVASIIGMPAVAKEWQENKSWNAHFTEHKSQGVVV
                     LWNENKQQGFTNNLKRANQAFLPASTFKIPNSLIALDLGVVKDEHQVFKWDGQTRDIAA
                     WNRDHDLITAMKYSVVPVYQEFARQIGEARMSKMLHAFDYGNEDISGNVDSFWLDGGIR
                     ISATQQIAFLRKLYHNKLHVSERSQRIVKQAMLTEANGDYIIRAKTGYSTSIEPKIGWW
                     VGWVELDDNVWFFAMNMDMPTSDGLGLRQAITKEVLKQEKIIP"
                     /name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
     gene            6726..7007
                     /locus_tag="U3T26_RS28350"
                     /old_locus_tag="U3T26_28350"
     CDS             6726..7007
                     /locus_tag="U3T26_RS28350"
                     /old_locus_tag="U3T26_28350"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_043907053.1"
                     /translation="MGFFNLSKALEEVSDAFGAKETAIASAKLLGKGVVNLAHFAATTG
                     LDSVVKQTSNKVLNSESSTEEQREQAKKSAAWADARIAARNKAENTED"
                     /name="hypothetical protein"
     gene            complement(7289..8743)
                     /locus_tag="U3T26_RS28355"
                     /old_locus_tag="U3T26_28355"
     CDS             complement(7289..8743)
                     /locus_tag="U3T26_RS28355"
                     /old_locus_tag="U3T26_28355"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_064996073.1"
                     /translation="MIIKFHNRGVGRGSGPVGYLLGRDGNREGATLDRGNPAVIEALID
                     SSPYAKKYTSGVLSFAEADLPRATKDKLMSDFEKALLPGLEGNQYAVLWVEHRDKGRLE
                     LNFVVPNIELQSGKRLQPYFHKADNPRLDAWRTVVNGELKLHDPDDPLNRQAVITPKDL
                     PRGHKEAAQAITDGLLRMAGAGEIQSRDDVRKALQGAGFSVVRETKNSLSIADPDGGRN
                     IRLKGVLYEQDFRHGEGLRAELEAAGQRYREASDRRIHEARESYLRGVEIKRGENERRY
                     CRAQPENGLAVAQNVDLDAGQHRPVLERNERDPLVSGGIDSGAASHSPSLGREGRQAKA
                     DPVREEIGAPLRADLRPQSNRLGEGYLLDRAEGVLNDGIGAGIALRVRGFAERIRAAAQ
                     GMAGKLRELGEHVREHQTRGRDQQGSSRAIDEAGERLNAASGRLEQAAGGLDRASQQVA
                     KEREHRLLKALDRGWERGDRSRGFER"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8724..9062)
                     /locus_tag="U3T26_RS28360"
                     /old_locus_tag="U3T26_28360"
     CDS             complement(8724..9062)
                     /locus_tag="U3T26_RS28360"
                     /old_locus_tag="U3T26_28360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_043907051.1"
                     /translation="MAGQPLKRLRSIKIRVSDAELERLRELCPKAQLAEWMREQCLGVA
                     QPQRRTPAPTVDPALLRQLAGMGNNLNQIARRVNSGEWGPLDRLRIIAELSAIGRELEE
                     LHHDHQIP"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(9044..9436)
                     /locus_tag="U3T26_RS28365"
                     /old_locus_tag="U3T26_28365"
     CDS             complement(9044..9436)
                     /locus_tag="U3T26_RS28365"
                     /old_locus_tag="U3T26_28365"
                     /inference="COORDINATES: protein motif:HMM:NF037437.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_043907050.1"
                     /translation="MSTTQIEKDSRRKAVNGVISSFQLEGEAITDEFKILLEDYINGKI
                     STDEVLAMVSDEPTTTEYDYEITPRTLGLGGGWNLRLLENGEEVGGGVFPLPEHCDFRD
                     EKALQTLLDSLYEDALAEASAWLASR"
                     /name="antitoxin VbhA family protein"
     gene            complement(9844..10791)
                     /locus_tag="U3T26_RS28370"
                     /old_locus_tag="U3T26_28370"
     CDS             complement(9844..10791)
                     /locus_tag="U3T26_RS28370"
                     /old_locus_tag="U3T26_28370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014293673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015060053.1"
                     /translation="MRKCAQLELFRERLPRKPYYSDELTTGLRIADVARALGARYIQPN
                     GPTHRHWIVFDVDHAAATLSWDDVGAPAPNITVTNKANGHAHLIYGLDTPIRTAPDGNA
                     APLRYAAAIEAALREKLGADMGYSGLICKNPLHEHWLVQVWEPRLYDLAWLSDYLDLSS
                     YNGRKSLPEYGLGRNCTLFEKTRLWAYKAIRQGWPDYPEWLAACVDRASGYNAQFEQPL
                     PANEVRHTAKSIAKWTHQHLSPAGFREEQARRGAKGGKVSKGGGRPSNSGKDKSDLLPE
                     VLRLKAQGYTNRDIADDLQISPSTVSVYLKRDRP"
                     /name="replication initiation protein"
     gene            <11167..11319
                     /locus_tag="U3T26_RS28375"
                     /old_locus_tag="U3T26_28375"
                     /pseudo=""
     CDS             <11167..11319
                     /locus_tag="U3T26_RS28375"
                     /old_locus_tag="U3T26_28375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015059985.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="EreA family erythromycin esterase"
                     /name="EreA family erythromycin esterase"
     ARG             complement(5589..6382)
                     /name="blaOXA-232"
                     /gene="blaOXA-232"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-232"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(11727..12280)
                     /name="OXA-232"
                     /gene="OXA-232"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="69.43"
     ARG             complement(3..241)
                     /name="OXA-788"
                     /gene="OXA-788"
                     /gene_name="OXA beta-lactamase; OXA-48-like beta-lactamase"
                     /tool="rgi"
                     /drug_class="carbapenem; penam"
                     /identity="100.0"
                     /coverage="30.65"
     Typing          complement(3717..4638)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(9858..10779)
                     /name="rep_cluster_1195"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.974"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(1152..2602)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7293..8743)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.554"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggcgatcaa gctattggga attttaaagg tagatgcggg taaaaatgct tggttcgccc
       61 gtttaagatt attggtaaat ccttgctgct tattctcatt ccagagcaca actacgccct
      121 gtgatttatg ttcagtaaag tgagcattcc aacttttgtt ttcttgccat tcctttgcta
      181 ccgctggcat tccgataatc gatgccacca aaaacacagc cgataaggct aatacacgca
      241 taacgtcccc ttgcttaatg caatatatcc atacaaatta agtgctaagc ttatcattct
      301 ctgccctctc attccctagc aaactgctga ccaattgtgg caacaatcag gcacaagctt
      361 taactattac ctaaattcca cgtgtgtttt ttattagctt caaaaatcac tatttcacga
      421 agaatttaga ctgcttctca cacattgtaa cattatttac aaccaccttt caatcatttt
      481 tgataaatca ttgatttcat ctttgctgca atgatactta ataaactctg caagttatcc
      541 acagagcaac actcaatttt attgatgata ttcttagggg gagtatggga ttttttaacc
      601 tatctaaagc gcttgaggag gtgtctgatg cttttggggc taaagaaacc gcaatcgcta
      661 gtgccaagtt gttaggtaag ggagttgtta acctagctca ttttgcagca acaacaggtt
      721 tggattcggt ggtcaaacaa acgagcaaca aagttctcaa cagcgagtct tcaaccgaag
      781 aacaacgaga acaggctaaa aagtctgccg catgggcaga cgcgagaatc gccgctagaa
      841 ataaagcaga aaacaccgaa gactagctga acgcacattt cgacaatgat gtggactaca
      901 tcgttgatca taacttcacg gtgcgctagc ttccatagaa gccaactaat aacaaggagt
      961 tagcatgtct gattcagatt tcaaaaatag ccctgagtat gatgactgga ttgaatctgg
     1021 tggtagagat gaagattaca gttactacta tgaaaaatgg catagaagaa ctcaacgata
     1081 attcccgccg ataaaaggaa agtgatagca ctttcctttt tttataactg acggccgttt
     1141 tgttccgtca acgctcaaag cctcgcgatc tgtctccacg ctcccagcct cgatccagtg
     1201 ccttcaggag cctgtgttcc ctttcttttg caacttgctg actagcccgg tcaagccctc
     1261 cagctgcttg ctcaagccgt ccacttgctg cgttaagccg ttcacctgcc tcgtcaatcg
     1321 ctctgctgct gccctgctga tctcggcctc ttgtttggtg ctctcgtaca tgctcgccaa
     1381 gttcgcgtag ctttcctgcc atgccttgtg ctgctgctcg tatgcgctct gcaaagcctc
     1441 gaacgcgcag agcaattccc gctccaatcc cgtcatttag taccccttcg gcacgatcca
     1501 ataggtatcc ttcccccaaa cggttggatt ggggtcggag atctgcacgc aaaggtgctc
     1561 caatttcttc ccgcactggg tcagctttag cttgccgccc ttctctgcca aggcttggag
     1621 agtggcttgc cgctccgcta tcaattcccc ctgataccag aggatcccgc tcgttgcgct
     1681 caagaacagg acgatgctga ccagcatcca gatccacgtt ttgagcaaca gccagcccat
     1741 tttctggctg tgctcggcaa tagcggcgct cattttctcc tcgcttgatt tcaacgcccc
     1801 ggagatagct ttctcgtgcc tcgtgaattc ttcggtcaga cgcttctcga tagcgttggc
     1861 ctgctgcttc gagctctgct cgaagtcctt cgccatgtct aaaatcttgc tcatacagca
     1921 ccccctttaa tcggatgttc cgccccccgt ccgggtcggc aatgctcagg ctgtttttgg
     1981 tttcgcgtac cacgctgaaa cccgcaccct gcaaggcttt gcgcacatca tcgcggctct
     2041 ggatctcccc tgctccggcc atgcgtaaaa gcccgtctgt aatggcctga gcggcctcct
     2101 tgtgccccct cggcaaatcc tttggcgtga taacggcctg acggtttaac ggatcgtccg
     2161 ggtcgtgcaa tttcagctcc ccgtttacca cggttcgcca cgcatcgagg cgcggattgt
     2221 cggccttgtg gaagtagggt tgcagccgct tcccgctctg caattcgatg ttcgggacta
     2281 caaaattcag ctccagccgc cccttgtcgc ggtgctctac ccacaaaaca gcgtactggt
     2341 tgccctccag tccgggcagg agcgcctttt caaagtcgct catcagcttg tccttggtcg
     2401 ccctgggtaa atcagcctcg gcaaacgaca gaacgccaga ggtgtatttt ttggcgtagg
     2461 gactggaatc aatcagggct tcaatcaccg cagggttgcc ccggtcgagc gttgcgcctt
     2521 cccggttccc gtcacggccc aacaagtagc caacaggccc ggagccccgg cctaccccac
     2581 ggttatggaa tttgatgatc atggtgcagt tcctccagct cgcggcctat agcggacagc
     2641 tccgctatga tgcgcagccg atccaacggc ccccactccc cggaattaac gcgccgggcg
     2701 atctggttca ggttgttgcc cattccggcc aactgccgca agagcgccgg atctacggta
     2761 ggcgccggcg ttcggcgctg gggttgcgca acaccgagac attgctcccg catccactcc
     2821 gccagttggg ccttggggca gagctcccgc aggcgctcaa gctccgcatc tgagacccgg
     2881 atcttgatag agcggaggcg ctttaacggc tggccagcca tgcagaggcc tccgctaagg
     2941 cgtcctcata gaggctatcg agtagcgtct ggagcgcctt ttcatcccga aaatcgcagt
     3001 gctccggcaa ggggaaaaca cccccgccga cctcttcgcc gttctccagc aaacgcagat
     3061 tccagccccc gccaagaccg agagtgcggg gggtgatttc gtagtcgtat tccgttgttg
     3121 tgggttcgtc acttaccata gctaacacct catcggtgga aatttttcca ttgatataat
     3181 cttcaagtag aattttgaat tcatcggtta tggcctcccc ctctagttgg aaactagaaa
     3241 taacgccatt gactgccttc cttctcgaat ctttctctat ctgtgttgtt gacatagggc
     3301 ctcgtccatt ctccagagcc aagaagccac cgcgcagacc gtcccgaagg ggcggatcga
     3361 gcgggggcca cagggggttt cgggggggcg tagcacccct gaacaaggcc acagaggccc
     3421 gagaccgcga agcaggtaga ggggcctctg tgtagccttg cttagtacat tattcaacca
     3481 tgggcgggat actgcaagcc ctagagcaag atcaagggaa agagcggctc cgtggataac
     3541 tgctgccgcc cacacccccc gaaaaaacag gggggcgatg gacggacagg cgcagttgtc
     3601 cacctcgcca aagatccgcg ctaaaaggat tttttaaggc taaaagacaa gagcgcagga
     3661 acccccctaa agggggctat tatctgataa ggcttgtttc gatcacggac gatcacgctt
     3721 taggtaaacg ctcaccgtac tgggggagat ttgaagatcg tccgctatat cgcggttggt
     3781 gtacccctga gccttaagcc gcagaacctc cggcaataga tcggacttgt ccttaccgct
     3841 attgctcggc cttccccctc ccttggatac cttgccgccc ttcgcacctc tccgggcctg
     3901 ctcctctctg aaccctgcgg ggcttaggtg ctggtgcgtc catttggcaa tggacttggc
     3961 cgtgtgccgg acttcgtttg cggggagcgg ctgctcgaac tgggcgttat atcctgatgc
     4021 ccggtcaaca caagcggcca accattcggg gtaatccggc cagccctgcc ggatcgcttt
     4081 ataggcccac aggcgcgttt tctcgaacag ggtacagttt cgcccaagcc cgtactcagg
     4141 aagcgatttg cggccattgt aggagctcag atcgaggtaa tcggatagcc aggctaaatc
     4201 gtagagccgg ggttcccata cctgcaccag ccagtgctca tgcagggggt tcttgcagat
     4261 cagcccggag tagcccatat ccgcccctaa tttctcccgg agcgcggcct cgatggcggc
     4321 ggcataccgc agaggggctg catttccatc cggggcagtg cggatcggcg tatccagtcc
     4381 gtagatcaga tgcgcgtggc cgttggcctt gttcgtgacg gtaatgttag gggctggagc
     4441 gcctacgtca tcccaagata acgtagcggc tgcatggtct acgtcaaaaa cgatccagtg
     4501 gcggtgcgtt ggcccgttcg gctggatgta acgcgcacca agggcgcggg caacatccgc
     4561 aatccgtagg cccgttgtaa gctcgtcgga atagtagggt ttccggggca ggcgctcccg
     4621 aaacagctca agctgtgcgc actttctcaa gcaccaccgc gcttattggc ggcgtggggt
     4681 tgtttggcat gatccatgtg atagacttct cttgtgattc gtcacggccc ccgtttttcg
     4741 cagctccgcc aagatgcaag cgataacggg gggttgtctt ttctggacaa tacgttacag
     4801 caggcacatc ctagagagca acgttagttg catgtaacct ttcaccctgg aacatatttc
     4861 cacctccttc tgcttctgcg ctgttttgaa ttgcttgatc tacgcctcta actaggtcga
     4921 tccttgggat tgagtggcac cattgtcagt gcaagaatac tgatccatat aagtctctct
     4981 agtcggacaa caccaagtgt tgaagagatc aaaaatcaac ctatatcgga ggattcatgt
     5041 ctgacattga cagatgcccc catgaaagca aagcgaatgc ggtcccaaag cgcctctgta
     5101 gaaacgaatt tgagcgaggc atttgatgcc atcgtctgcg tcccaagcgc cggcaaggac
     5161 ggcctggttg acctatagta aaccggtaga tgaaaatgat ggagaaccaa tatcagcgcc
     5221 ttcttggact ttttcgtcaa acgctttagc agccaagaaa gcctacaatc cttgatgggc
     5281 taggcacttt atcgcagcgg cattcacctt tactgacatc gccactcctt tattcacacc
     5341 cccatgttga gtgaccatgg ccattgagat aacaaaaaag cctagcaaat tggctaggct
     5401 ttttacgttt aggaatgtga tgctaatgct caggctgaaa gttaacgcgc ggagcttaag
     5461 tcgattaggc ctgaccgatt aagtatgagc cattaactac aagcgcatcg agcatcagca
     5521 ttttgtccat atatctttac agcttatatg acggctattt cacacaaata cgcgctaacc
     5581 acttctaggg aataattttc tcctgtttga gcacttcttt tgtgatggct tggcgcagcc
     5641 ctaaaccatc cgatgtgggc atatccatat tcatcgcaaa aaaccacaca ttatcatcaa
     5701 gttcaaccca accaacccac cagccaatct taggttcgat actagtcgag tatcccgttt
     5761 tagcccgaat aatatagtcg ccattggctt cggtcagcat ggcttgtttc acgatgcgct
     5821 gactacgctc agaaacgtgc agcttgttgt gatacagctt gcgtaaaaaa gcgatttgct
     5881 gggtagccga aatgcgaata ccaccatcga gccaaaaact gtctacattg cccgagatat
     5941 cctcattgcc ataatcgaag gcgtgcagca ttttactcat acgtgcctca ccaatttggc
     6001 gggcaaattc ttgataaaca ggcacaactg agtacttcat cgcggtaatt aagtcatggt
     6061 cacgattcca agcggcgata tcacgcgtct gtccatccca cttaaagact tggtgttcat
     6121 ccttaaccac gcccaaatcg agggcgatca agctattggg aattttaaag gtagatgcgg
     6181 gtaaaaatgc ttggttcgcc cgtttaagat tattggtaaa tccttgctgc ttattctcat
     6241 tccagagcac aactacgccc tgtgatttat gttcagtaaa gtgagcattc caacttttgt
     6301 tttcttgcca ttcctttgct accgctggca ttccgataat cgatgccacc aaaaacacag
     6361 ccgataaggc taatacacgc ataacgtccc cttgcttaat gcaatatatc catacaaatt
     6421 aagtgctaag cttatcattc tctgccctct cattccctag caaactgctg accaattgtg
     6481 gcaacaatca ggcacaagct ttaactatta cctaaattcc acgtgtgttt tttattagct
     6541 tcaaaaatca ctatttcacg aagaatttag actgcttctc acacattgta acattattta
     6601 caaccacctt tcaatcattt ttgataaatc attgatttca tctttgctgc aatgatactt
     6661 aataaactct gcaagttatc cacagagcaa cactcaattt tattgatgat attcttaggg
     6721 ggagtatggg attttttaac ctatctaaag cgcttgagga ggtgtctgat gcttttgggg
     6781 ctaaagaaac cgcaatcgct agtgccaagt tgttaggtaa gggagttgtt aacctagctc
     6841 attttgcagc aacaacaggt ttggattcgg tggtcaaaca aacgagcaac aaagttctca
     6901 acagcgagtc ttcaaccgaa gaacaacgag aacaggctaa aaagtctgcc gcatgggcag
     6961 acgcgagaat cgccgctaga aataaagcag aaaacaccga agactagctg aacgcacatt
     7021 tcgacaatga tgtggactac atcgttgatc ataacttcac ggtgcgctag cttccataga
     7081 agccaactaa taacaaggag ttagcatgtc tgattcagat ttcaaaaata gccctgagta
     7141 tgatgactgg attgaatctg gtggtagaga tgaagattac agttactact atgaaaaatg
     7201 gcatagaaga actcaacgat aattcccgcc gataaaagga aagtgatagc actttccttt
     7261 ttttataact gacggccgtt ttgttccgtc aacgctcaaa gcctcgcgat ctgtctccac
     7321 gctcccagcc tcgatccagt gccttcagga gcctgtgttc cctttctttt gcaacttgct
     7381 gactagcccg gtcaagccct ccagctgctt gctcaagccg tccacttgct gcgttaagcc
     7441 gttcacctgc ctcgtcaatc gctctgctgc tgccctgctg atctcggcct cttgtttggt
     7501 gctctcgtac atgctcgcca agttcgcgta gctttcctgc catgccttgt gctgctgctc
     7561 gtatgcgctc tgcaaagcct cgaacgcgca gagcaattcc cgctccaatc ccgtcattta
     7621 gtaccccttc ggcacgatcc aataggtatc cttcccccaa acggttggat tggggtcgga
     7681 gatctgcacg caaaggtgct ccaatttctt cccgcactgg gtcagcttta gcttgccgcc
     7741 cttctctgcc aaggcttgga gagtggcttg ccgctccgct atcaattccc cctgatacca
     7801 gaggatcccg ctcgttgcgc tcaagaacag gacgatgctg accagcatcc agatccacgt
     7861 tttgagcaac agccagccca ttttctggct gtgctcggca atagcggcgc tcattttctc
     7921 ctcgcttgat ttcaacgccc cggagatagc tttctcgtgc ctcgtgaatt cttcggtcag
     7981 acgcttctcg atagcgttgg cctgctgctt cgagctctgc tcgaagtcct tcgccatgtc
     8041 taaaatcttg ctcatacagc acccccttta atcggatgtt ccgccccccg tccgggtcgg
     8101 caatgctcag gctgtttttg gtttcgcgta ccacgctgaa acccgcaccc tgcaaggctt
     8161 tgcgcacatc atcgcggctc tggatctccc ctgctccggc catgcgtaaa agcccgtctg
     8221 taatggcctg agcggcctcc ttgtgccccc tcggcaaatc ctttggcgtg ataacggcct
     8281 gacggtttaa cggatcgtcc gggtcgtgca atttcagctc cccgtttacc acggttcgcc
     8341 acgcatcgag gcgcggattg tcggccttgt ggaagtaggg ttgcagccgc ttcccgctct
     8401 gcaattcgat gttcgggact acaaaattca gctccagccg ccccttgtcg cggtgctcta
     8461 cccacaaaac agcgtactgg ttgccctcca gtccgggcag gagcgccttt tcaaagtcgc
     8521 tcatcagctt gtccttggtc gccctgggta aatcagcctc ggcaaacgac agaacgccag
     8581 aggtgtattt tttggcgtag ggactggaat caatcagggc ttcaatcacc gcagggttgc
     8641 cccggtcgag cgttgcgcct tcccggttcc cgtcacggcc caacaagtag ccaacaggcc
     8701 cggagccccg gcctacccca cggttatgga atttgatgat catggtgcag ttcctccagc
     8761 tcgcggccta tagcggacag ctccgctatg atgcgcagcc gatccaacgg cccccactcc
     8821 ccggaattaa cgcgccgggc gatctggttc aggttgttgc ccattccggc caactgccgc
     8881 aagagcgccg gatctacggt aggcgccggc gttcggcgct ggggttgcgc aacaccgaga
     8941 cattgctccc gcatccactc cgccagttgg gccttggggc agagctcccg caggcgctca
     9001 agctccgcat ctgagacccg gatcttgata gagcggaggc gctttaacgg ctggccagcc
     9061 atgcagaggc ctccgctaag gcgtcctcat agaggctatc gagtagcgtc tggagcgcct
     9121 tttcatcccg aaaatcgcag tgctccggca aggggaaaac acccccgccg acctcttcgc
     9181 cgttctccag caaacgcaga ttccagcccc cgccaagacc gagagtgcgg ggggtgattt
     9241 cgtagtcgta ttccgttgtt gtgggttcgt cacttaccat agctaacacc tcatcggtgg
     9301 aaatttttcc attgatataa tcttcaagta gaattttgaa ttcatcggtt atggcctccc
     9361 cctctagttg gaaactagaa ataacgccat tgactgcctt ccttctcgaa tctttctcta
     9421 tctgtgttgt tgacataggg cctcgtccat tctccagagc caagaagcca ccgcgcagac
     9481 cgtcccgaag gggcggatcg agcgggggcc acagggggtt tcgggggggc gtagcacccc
     9541 tgaacaaggc cacagaggcc cgagaccgcg aagcaggtag aggggcctct gtgtagcctt
     9601 gcttagtaca ttattcaacc atgggcggga tactgcaagc cctagagcaa gatcaaggga
     9661 aagagcggct ccgtggataa ctgctgccgc ccacaccccc cgaaaaaaca ggggggcgat
     9721 ggacggacag gcgcagttgt ccacctcgcc aaagatccgc gctaaaagga ttttttaagg
     9781 ctaaaagaca agagcgcagg aaccccccta aagggggcta ttatctgata aggcttgttt
     9841 cgatcacgga cgatcacgct ttaggtaaac gctcaccgta ctgggggaga tttgaagatc
     9901 gtccgctata tcgcggttgg tgtacccctg agccttaagc cgcagaacct ccggcaatag
     9961 atcggacttg tccttaccgc tattgctcgg ccttccccct cccttggata ccttgccgcc
    10021 cttcgcacct ctccgggcct gctcctctct gaaccctgcg gggcttaggt gctggtgcgt
    10081 ccatttggca atggacttgg ccgtgtgccg gacttcgttt gcggggagcg gctgctcgaa
    10141 ctgggcgtta tatcctgatg cccggtcaac acaagcggcc aaccattcgg ggtaatccgg
    10201 ccagccctgc cggatcgctt tataggccca caggcgcgtt ttctcgaaca gggtacagtt
    10261 tcgcccaagc ccgtactcag gaagcgattt gcggccattg taggagctca gatcgaggta
    10321 atcggatagc caggctaaat cgtagagccg gggttcccat acctgcacca gccagtgctc
    10381 atgcaggggg ttcttgcaga tcagcccgga gtagcccata tccgccccta atttctcccg
    10441 gagcgcggcc tcgatggcgg cggcataccg cagaggggct gcatttccat ccggggcagt
    10501 gcggatcggc gtatccagtc cgtagatcag atgcgcgtgg ccgttggcct tgttcgtgac
    10561 ggtaatgtta ggggctggag cgcctacgtc atcccaagat aacgtagcgg ctgcatggtc
    10621 tacgtcaaaa acgatccagt ggcggtgcgt tggcccgttc ggctggatgt aacgcgcacc
    10681 aagggcgcgg gcaacatccg caatccgtag gcccgttgta agctcgtcgg aatagtaggg
    10741 tttccggggc aggcgctccc gaaacagctc aagctgtgcg cactttctca agcaccaccg
    10801 cgcttattgg cggcgtgggg ttgtttggca tgatccatgt gatagacttc tcttgtgatt
    10861 cgtcacggcc cccgtttttc gcagctccgc caagatgcaa gcgataacgg ggggttgtct
    10921 tttctggaca atacgttaca gcaggcacat cctagagagc aacgttagtt gcatgtaacc
    10981 tttcaccctg gaacatattt ccacctcctt ctgcttctgc gctgttttga attgcttgat
    11041 ctacgcctct aactaggtcg atccttggga ttgagtggca ccattgtcag tgcaagaata
    11101 ctgatccata taagtctctc tagtcggaca acaccaagtg ttgaagagat caaaaatcaa
    11161 cctatatcgg aggattcatg tctgacattg acagatgccc ccatgaaagc aaagcgaatg
    11221 cggtcccaaa gcgcctctgt agaaacgaat ttgagcgagg catttgatgc catcgtctgc
    11281 gtcccaagcg ccggcaagga cggcctggtt gacctatagt aaaccggtag atgaaaatga
    11341 tggagaacca atatcagcgc cttcttggac tttttcgtca aacgctttag cagccaagaa
    11401 agcctacaat ccttgatggg ctaggcactt tatcgcagcg gcattcacct ttactgacat
    11461 cgccactcct ttattcacac ccccatgttg agtgaccatg gccattgaga taacaaaaaa
    11521 gcctagcaaa ttggctaggc tttttacgtt taggaatgtg atgctaatgc tcaggctgaa
    11581 agttaacgcg cggagcttaa gtcgattagg cctgaccgat taagtatgag ccattaacta
    11641 caagcgcatc gagcatcagc attttgtcca tatatcttta cagcttatat gacggctatt
    11701 tcacacaaat acgcgctaac cacttctagg gaataatttt ctcctgtttg agcacttctt
    11761 ttgtgatggc ttggcgcagc cctaaaccat ccgatgtggg catatccata ttcatcgcaa
    11821 aaaaccacac attatcatca agttcaaccc aaccaaccca ccagccaatc ttaggttcga
    11881 tactagtcga gtatcccgtt ttagcccgaa taatatagtc gccattggct tcggtcagca
    11941 tggcttgttt cacgatgcgc tgactacgct cagaaacgtg cagcttgttg tgatacagct
    12001 tgcgtaaaaa agcgatttgc tgggtagccg aaatgcgaat accaccatcg agccaaaaac
    12061 tgtctacatt gcccgagata tcctcattgc cataatcgaa ggcgtgcagc attttactca
    12121 tacgtgcctc accaatttgg cgggcaaatt cttgataaac aggcacaact gagtacttca
    12181 tcgcggtaat taagtcatgg tcacgattcc aagcggcgat atcacgcgtc tgtccatccc
    12241 acttaaagac ttggtgttca tccttaacca cgcccaaatc ga
//
