LOCUS       NZ_CP139942             6079 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Acinetobacter pittii strain CARB128 plasmid pCARB128_3, complete
            sequence.
ACCESSION   NZ_CP139942
VERSION     NZ_CP139942.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38610277
            Assembly: GCF_034424115.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter pittii
  ORGANISM  Acinetobacter pittii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-OCT-2020
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 88.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 12:40:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,216
            CDSs (total)                      :: 4,120
            Genes (coding)                    :: 3,951
            CDSs (with protein)               :: 3,951
            Genes (RNA)                       :: 96
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 169
            CDSs (without protein)            :: 169
            Pseudo Genes (ambiguous residues) :: 0 of 169
            Pseudo Genes (frameshifted)       :: 90 of 169
            Pseudo Genes (incomplete)         :: 73 of 169
            Pseudo Genes (internal stop)      :: 25 of 169
            Pseudo Genes (multiple problems)  :: 16 of 169
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139942.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Acinetobacter pittii"
                     /mol_type="genomic DNA"
                     /strain="CARB128"
                     /host="Homo sapiens"
                     /db_xref="taxon:48296"
                     /plasmid="pCARB128_3"
                     /geo_loc_name="Germany"
                     /collection_date="2020-05-12"
     gene            32..>99
                     /locus_tag="U0544_RS00265"
                     /old_locus_tag="U0544_00265"
                     /pseudo=""
     CDS             32..>99
                     /locus_tag="U0544_RS00265"
                     /old_locus_tag="U0544_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            212..523
                     /locus_tag="U0544_RS00270"
                     /old_locus_tag="U0544_00270"
     CDS             212..523
                     /locus_tag="U0544_RS00270"
                     /old_locus_tag="U0544_00270"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227552063.1"
                     /translation="MKIPDDLKDQLTEYRNIPILQKNRLLHLKGGRINTATLFILLSKI
                     SKYKLLNGLKKIYIEEQSKVKILSTFKLLEEIKNLVDTNTAFKQNNGALPHPIERLDS"
                     /name="hypothetical protein"
     gene            614..1147
                     /gene="aadB"
                     /locus_tag="U0544_RS00275"
                     /old_locus_tag="U0544_00275"
     CDS             614..1147
                     /gene="aadB"
                     /locus_tag="U0544_RS00275"
                     /old_locus_tag="U0544_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /protein_id="WP_010883707.1"
                     /translation="MDTTQVALIHQILAAADERNLPLWIGGGWAIDARLGRVTRKHDDI
                     DLTFPGERRGELEAIVEMLGGRVTEELDYGFLAEIGDELLDCEPAWWADEAYEIAEAPQ
                     GSCPEAAEGVIAGRPVRCNSWEAIIWDYFYYADEVPPVDWPTKHIESYRFACTSLGAEK
                     VEVLRAAFRSRYAA"
                     /name="aadB"
     gene            complement(1306..1974)
                     /locus_tag="U0544_RS00280"
                     /old_locus_tag="U0544_00280"
     CDS             complement(1306..1974)
                     /locus_tag="U0544_RS00280"
                     /old_locus_tag="U0544_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="WP_000423610.1"
                     /translation="MELSNDAVLKIEQALSIPRLSKYENFYKDKGEPYEKSDVLMLYER
                     NLIISNKFFYLLNYFEVVLRNAVVQAIEISFRCNETNSWHENEAFIRSLSRRGRYSPKS
                     MFDSAKEKFPDSPSKMIPELKFVFWQKMLMANYEERIWQGCFNSIFPNATVENRQIFYD
                     WTDQLRELRNRIAHHEPIIFNRNLENDLEVLTKFIYCRCINTYDFIEQSALDLKEYLDF
                     "
                     /name="Abi family protein"
     gene            complement(2107..2592)
                     /locus_tag="U0544_RS00285"
                     /old_locus_tag="U0544_00285"
     CDS             complement(2107..2592)
                     /locus_tag="U0544_RS00285"
                     /old_locus_tag="U0544_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005200081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_051064109.1"
                     /translation="MNNNCVVVLTARGKTRILKEGGSQSWRLNAHNAGKCDYLVCVQNR
                     KQSWGEPEADHHTAFLVGKISSISQSQEEDADGRWIINISEYADISIPNMWDGNRNPVS
                     YSSLEEMNIDLNELKFTKLNEDNSSKTLDKISIPEAKRLLANYFDVPQENISITINM"
                     /name="hypothetical protein"
     gene            complement(2701..4515)
                     /locus_tag="U0544_RS00290"
                     /old_locus_tag="U0544_00290"
     CDS             complement(2701..4515)
                     /locus_tag="U0544_RS00290"
                     /old_locus_tag="U0544_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_000634474.1"
                     /translation="MIVDFFRHGSGLSKGCLDYLLGEDREREHAQVLNGDVELTAQLID
                     SSPFAKKYTSGCLSFYEHDLSDQDKQKIMQNFEECLFPGLDKDQYQILWVQHQDKINQD
                     TGETRLELNFVIPNVELSTGKRLQPFYAPVDLDRVDLFKQITNTEHSLYDPDDPEHRQL
                     FLNKKNLPKDIKDFKAQLHQRVYRAVANGDVADRQELVQWLESNQINVTRQVKNSISIE
                     NPYEGAKRPIRLEGEIYEQGFRATGEYRQEVQQRIEEYRGTTSERYRTNVTDYQRQLEH
                     KSQYHCDRYPTVGRENSPEHSKQRPDGREAVKPVSNLAAIEIEPFNAIKRANTEPRTTS
                     PESSGTEKTYHFEYGTDFSSSYFAYSNFLAWSRHQKQVQRDADAKYRDQREANEGRPFE
                     PIGRQFDDQHMQATRQQSTAPMYSDQQERRRMAERLHDSNGVLNDDRIRNTIIENHRRT
                     TATINATTSAIAEATASFRHNANQNYPSLVSTIKDNQERSAKLTASTEIISDNAEAISR
                     ARTKYSKLYRADQWQSPRDEPYHSQQRRNSGENLNHTAFCLVRSSQSAAEFARNIRDIE
                     KNLVQMNERKQEIEKPRPKQDRGMDFGM"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4505..4912)
                     /locus_tag="U0544_RS00295"
                     /old_locus_tag="U0544_00295"
     CDS             complement(4505..4912)
                     /locus_tag="U0544_RS00295"
                     /old_locus_tag="U0544_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000016535.1"
                     /translation="MSGQEQENKQSKAVRERTFKVRLSESEYQLLDSNNPYGSIAKLLR
                     QAAVEKATKIHNSKAEDDQKIKATKVVESSYSKTERALILELGRIGNNVNQIAKAVNTD
                     IAGVGTFDKVKLLHLLISIDQQLRELRPDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     ARG             615..1144
                     /name="ant(2'')-Ia"
                     /gene="ant(2'')-Ia"
                     /gene_name="aminoglycoside nucleotidyltransferase
                     ANT(2'')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.31"
                     /coverage="100.0"
     ARG             603..1147
                     /name="ANT(2'')-Ia"
                     /gene="ANT(2'')-Ia"
                     /gene_name="ANT(2'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="97.74"
                     /coverage="102.26"
     Typing          complement(2705..4515)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaattgtt ttatctgatt tttcaggagt aatcttaaat aagaataggt ttacgaatgt
       61 tcacttcaaa aagtgcgtat tttatgctgc tatttttaga gattgtcgct ttaaaaattg
      121 cacatttgaa aaatgcattt ttattaatac aaatacagaa agcctaaatc actcgatatt
      181 agactgttct aaaaaatata atttttacga aatgaaaatt cctgatgact taaaagatca
      241 actaacagaa tatagaaata ttccaatact gcaaaaaaac cgtttacttc atctaaaagg
      301 gggaagaatc aataccgcaa ccttattcat tctcctatcc aaaatatcca aatacaagct
      361 gctaaatggt cttaaaaaaa tttatattga ggaacaaagt aaagtaaaaa tacttagcac
      421 tttcaaactc cttgaagaaa taaagaacct agttgacact aacaccgcat tcaaacagaa
      481 taatggtgct ctgccccatc caatcgaaag gttggatagt taagacaatc actgggagct
      541 cactattgga tgtaacaatt caattaatag tctaattact gacattctgg gagggcttac
      601 tatgttaggc cgcatggaca caacgcaggt cgcattgata caccaaattc tagctgcggc
      661 agatgagcga aatctgccgc tctggatcgg tgggggctgg gcgatcgatg cacggctagg
      721 gcgtgtaaca cgcaagcacg atgatattga tctgactttt cccggcgaga ggcgcggcga
      781 gctcgaggca atagttgaaa tgctcggcgg gcgcgtcacg gaggagttgg actatggatt
      841 cttagcggag atcggggatg agttacttga ctgcgaacct gcttggtggg cagacgaagc
      901 gtatgaaatc gcggaggctc cgcagggctc gtgcccagag gcggctgagg gtgtcatcgc
      961 cgggcggcca gtccgttgta acagctggga ggcgatcatc tgggattact tttactatgc
     1021 cgatgaagta ccaccagtgg actggcctac aaagcacata gagtcctaca ggttcgcatg
     1081 cacctcactc ggggcggaaa aggttgaggt cttgcgtgcc gctttcaggt cgcgatatgc
     1141 ggcctaacaa ttcgtccaag ccgacgccgc tttgcggcgc ggcttaactc aggtgttagg
     1201 aaacactgct accgttatcg gatttactgc tttactacta accttgttaa ttcaacattt
     1261 gcaaaatagt ctgacctttc gattgtttgg ggtgaaattc ttatcttaaa aatctaaata
     1321 ttcttttaga tctaatgcag attgctcgat aaaatcgtat gtattaatac aacgacagta
     1381 aataaatttt gttaaaacct ctaaatcatt ttctagatta cggttaaaaa taatcggttc
     1441 atgatgagca atcctgtttc ttaattcacg taattgatct gtccaatcgt aaaaaatttg
     1501 cctgttttca actgttgcat taggaaaaat agaattaaaa catccctgcc aaatacgttc
     1561 ttcataatta gccataagca tcttttgcca gaatacaaat ttaagctcag ggatcatttt
     1621 actaggcgaa tctgggaact tctctttagc actatcaaac atacttttag ggctatacct
     1681 ccctctacgg ctaaggcttc gaataaaagc ttcattttca tgccaactat ttgtttcatt
     1741 acacctaaaa gatatttcta ttgcctgaac aacagcattt ctcagaacaa cttcaaaata
     1801 atttaataag taaaaaaact tattggaaat tattaaatta cgttcataaa gcatcaatac
     1861 atcagatttt tcatatggtt cacctttatc tttatagaaa ttttcatact tagataagcg
     1921 tggtatagat aaagcctgct ctatcttaag cactgcatca tttgacaatt ccaccgtata
     1981 taccctaata tatagttgta agtttgagag actgcggatc taagatcttc cctcccaagc
     2041 gctaagattc aaaaaagcct ttcattaacg tgaaaggctt ttttcttttt ttggtcaatt
     2101 aaaagcttac atattgattg taattgaaat attttcttga gggacatcaa aataattagc
     2161 taatagtctc ttagcttcag gaatagatat tttatccagt gttttagaag aattatcttc
     2221 atttaatttg gtaaatttca gctcattcaa atcgatattc atctcttcta atgaactata
     2281 agaaactgga ttccgattac catcccacat attgggaata gatatatcag catattcgga
     2341 gatatttatt atccatctac catctgcatc ttcttcttga gattggctga tactagatat
     2401 tttgccaact aaaaatgcag tgtgatgatc agcctctggt tctccccaag attgtttcct
     2461 attttgcaca caaactagat aatcgcactt tcctgcatta tgagcattta gtctccaaga
     2521 ctgactacca ccttctttta aaattctagt ctttccacga gcagttagta ctactacaca
     2581 gttattattc ataacaccac caaaatatat aaacaagcct atactacatt caatatagct
     2641 aaatttaaac attagtacaa gctaatacta agaaataaac accaacatac tagaaaataa
     2701 ttacatccca aaatccatac ctcgatcttg cttaggtcga ggtttttcta tctcttgttt
     2761 acgctcattc atctggacga gatttttttc aatatctcgg atgtttcgag caaattctgc
     2821 tgcgctttgg ctacttctga cgagacagaa tgctgtgtgg ttgagattct ctccagaatt
     2881 tcttcgttgt tggctgtgat aaggctcatc tctcggactt tgccattgat ctgctcggta
     2941 tagcttgctg tacttcgttc tagctcgtga aattgcttct gcattgtcgc taatgatttc
     3001 tgtgcttgct gtaagctttg ctgatcgctc ttgattgtct ttgattgtgc tgacaaggct
     3061 tggataattt tggtttgcat tgtgtctaaa gcttgctgtt gcttctgcaa ttgcacttgt
     3121 tgttgcatta attgttgctg ttgttcttcg atgattctca ataattgtgt ttctaattcg
     3181 gtcatcgttt aataccccat tggaatcatg tagccgttct gccattcgcc tgcgttcttg
     3241 ttgatctgaa tacataggcg ctgtgctttg ctgtctggtc gcttgcatgt gctgatcgtc
     3301 aaactgcctc ccgatcggtt caaatggtcg accctcattt gcttctcgtt gatcacggta
     3361 tttagcgtct gcatctcgct gtacttgttt ttggtgacga gaccaagcga gaaaattgct
     3421 ataagcaaaa taactgctac tgaaatcagt cccatattca aagtggtaag ttttttctgt
     3481 accgcttgat tctgggcttg ttgttcttgg ctctgtattt gctcgtttaa ttgcgttaaa
     3541 tggctcaatt tcgattgcag caaggtttga tacaggctta actgcttctc gaccgtctgg
     3601 gcgttgtttt gaatgctctg ggctattttc tcgtccgact gttggatagc ggtcgcagtg
     3661 atattggctc ttatgttcaa gctgtctctg atagtctgtg acgtttgttc gataccgctc
     3721 agaagttgtt cctctgtatt cttcaattcg ttgttgtact tcttgtctat attcgccagt
     3781 agctctaaag ccttgctcat aaatctcgcc ctctaaacgg atgggacgtt ttgcgccctc
     3841 atatgggttt tcaatggata tggagttttt tacttgtcga gtgacattga tttgattcga
     3901 ttcaagccat tgcaccaatt cttgccgatc ggcaacatca ccattggcaa ccgctcggta
     3961 aacacgttga tgtaactgcg ctttaaaatc cttaatatct ttgggtaggt tctttttatt
     4021 taaaaatagc tgtcgatgct caggatcatc ggggtcatac agtgaatgtt cggtattagt
     4081 gatctgctta aatagatcga ctcgatctaa atcgactggc gcatagaatg gctgtaggcg
     4141 tttgcctgtt gaaagctcaa cattaggaat cacaaaatta agctcaagcc ttgtctcacc
     4201 tgtgtcctga ttaattttgt cttggtgctg tacccataaa atttgatatt ggtctttatc
     4261 tagtcccggg aataaacatt cctcaaaatt ttgcatgatt ttctgtttat cctggtcaga
     4321 taaatcatgc tcataaaagg acaaacaacc actggtatat tttttggcaa aaggactgct
     4381 atcaatgagt tgtgctgtca gttctacatc accattgagc acttgagcat gttcacgctc
     4441 tcgatcttca cctaacaaat aatcgagaca gccttttgat aaacctgaac catgtctaaa
     4501 gaaatcaacg atcatctgga cgtaactccc gaagctgctg atctatggaa atcaacaaat
     4561 gaagtaactt cactttgtca aaagtaccta cccctgcaat atcggtattc actgctttag
     4621 caatctgatt cacattgttg ccaatccgac caagctctaa aattaatgct cgctctgtct
     4681 tgctatacga agattcaaca acctttgttg ctttaatttt ttggtcatct tctgctttgg
     4741 aattatgaat tttagtcgct ttctctactg ctgcttgtct aagcaacttt gcaatcgaac
     4801 cataaggatt attggaatcg agcaattgat attctgattc tgaaagtctg actttaaagg
     4861 ttcgttctcg aactgctttg ctctgtttgt tttcttgttc ctgtcctgac atttcaatct
     4921 accctgcttt ggcttttgta agctttaaat tggaaaatct ttgatttttc tgtttaaagc
     4981 ttttcggggg tatcgggggt gaagcccctg atcaaggtca cagactatca ctgtttaacg
     5041 aagttaaata ggggtagctg tgtatccttg cttatttaaa aatggacgag tgaaattcaa
     5101 taaaaactaa tcaaatgaca cggtatataa actctagtag gtattggcat gaatcttgta
     5161 aaaacaaatg cgctcaactc tttctcaccg tccccacatc cctcgctttg ctcgggctga
     5221 tggtgaacgt tgagagagtt tcggcaatac tttccctaag aggctttgat ttaaaaaaat
     5281 caaatatcct cttaagtatt gccgattaac ctgtaattgg gctgacggag ccacgcatcg
     5341 gtcgtatccg cacatggttt tgctgaccat gcagacaagg actaaaggtt ttgcaccttc
     5401 tcccacgcgc tccctgtcga agtatggtgg tatctctgcg aggattactc caacatgcaa
     5461 cttaaccaac ctatacaact acttccaacg ctccgcctat gtgagatccc ctgtaagcgg
     5521 tttagccttg cgactatcgc tacgatgcac cagtgacact tattccctcc acatgcaacg
     5581 catatcttcc ttaaaaaagg cgcacagaag tagataaagc tcgaccgtta ggtgcttatt
     5641 tgatgattta aactgctgaa ttaacgaagg acttcaaatt gaggcttgtc aaactgttct
     5701 aattctttaa aatagatagt ggtactagcc tttaaggtga atatcagtat gagttgcaag
     5761 ctctgccgat ctttcgttaa ttgatgttgc aagcatcaaa taactgtacc catcgaattt
     5821 aaaactaatg gacagatttc gcaagagacc tgtccattat tttttttgat tacacctaag
     5881 aattaagctg aaactcctat tctttggact attaatgtca aaacttccta gatttcataa
     5941 caagcacatc gaaaaacgta agtattcaaa tagcctccta gccaaatcta tttcttctaa
     6001 agtacgtttc tctaataaaa cattttttaa tgttaatttg agaggatccc gcctacgatc
     6061 atgttcattc aaaaatata
//
