LOCUS       NZ_CP139857             9544 bp    DNA     circular CON 21-DEC-2023
DEFINITION  Escherichia coli strain Nord122 plasmid pNord122_4, complete
            sequence.
ACCESSION   NZ_CP139857
VERSION     NZ_CP139857.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38611015
            Assembly: GCF_034422495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9544)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9544)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-OCT-2018
            Assembly Method                   :: Flye v. 2.9.1-b1780
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 87.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 06:10:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,407
            CDSs (total)                      :: 5,281
            Genes (coding)                    :: 5,020
            CDSs (with protein)               :: 5,020
            Genes (RNA)                       :: 126
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 261
            CDSs (without protein)            :: 261
            Pseudo Genes (ambiguous residues) :: 0 of 261
            Pseudo Genes (frameshifted)       :: 121 of 261
            Pseudo Genes (incomplete)         :: 147 of 261
            Pseudo Genes (internal stop)      :: 41 of 261
            Pseudo Genes (multiple problems)  :: 46 of 261
            Pseudo Genes (short protein)      :: 4 of 261
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139857.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Nord122"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pNord122_4"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            <1..573
                     /locus_tag="U0530_RS26990"
                     /old_locus_tag="U0530_26985"
                     /pseudo=""
     CDS             <1..573
                     /locus_tag="U0530_RS26990"
                     /old_locus_tag="U0530_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(777..1175)
                     /locus_tag="U0530_RS26995"
                     /old_locus_tag="U0530_26990"
     CDS             complement(777..1175)
                     /locus_tag="U0530_RS26995"
                     /old_locus_tag="U0530_26990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            1352..2278
                     /locus_tag="U0530_RS27000"
                     /old_locus_tag="U0530_26995"
     CDS             1352..2278
                     /locus_tag="U0530_RS27000"
                     /old_locus_tag="U0530_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            2281..2814
                     /locus_tag="U0530_RS27005"
                     /old_locus_tag="U0530_27000"
     CDS             2281..2814
                     /locus_tag="U0530_RS27005"
                     /old_locus_tag="U0530_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(2880..3089)
                     /locus_tag="U0530_RS27010"
                     /old_locus_tag="U0530_27005"
     CDS             complement(2880..3089)
                     /locus_tag="U0530_RS27010"
                     /old_locus_tag="U0530_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            4825..5739
                     /locus_tag="U0530_RS27015"
                     /old_locus_tag="U0530_27010"
     CDS             4825..5739
                     /locus_tag="U0530_RS27015"
                     /old_locus_tag="U0530_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            complement(5943..6341)
                     /locus_tag="U0530_RS27020"
                     /old_locus_tag="U0530_27015"
     CDS             complement(5943..6341)
                     /locus_tag="U0530_RS27020"
                     /old_locus_tag="U0530_27015"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            6518..7444
                     /locus_tag="U0530_RS27025"
                     /old_locus_tag="U0530_27020"
     CDS             6518..7444
                     /locus_tag="U0530_RS27025"
                     /old_locus_tag="U0530_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            7447..7980
                     /locus_tag="U0530_RS27030"
                     /old_locus_tag="U0530_27025"
     CDS             7447..7980
                     /locus_tag="U0530_RS27030"
                     /old_locus_tag="U0530_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(8046..8255)
                     /locus_tag="U0530_RS27035"
                     /old_locus_tag="U0530_27030"
     CDS             complement(8046..8255)
                     /locus_tag="U0530_RS27035"
                     /old_locus_tag="U0530_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     Typing          2..573
                     /name="rep_cluster_2131"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.137"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4826..5600
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          1353..2275
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6519..7441
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgtgtgaga aactttgtgc ggatgtgaac tacagcggcc tgatttgcaa aaatccgttc
       61 cacctggaat ggctggtgat ggagtggcgc gaggaagcct ataccctcga tgaactggct
      121 gattatctcg atttgagcgc ctcagagcgt cgtagcatcg ataaacatta cgggatgggg
      181 cgaaactgcc acctgttcga aatgacgcgc aaatgggctt acagggcgat tcgtcagggc
      241 tggccagcgt tctcacagtg gcttgaagcg gtgatccagc gtgtcgaaat gtacaatgca
      301 tcgcttcccg ttccgctttc actggctgaa tgtcgggcta ttggcaagag cattgcgaaa
      361 tacacgcaca ggaacttcac gccggaaact ttcgcacagt atgtggctga tacgcacacg
      421 ccagaaattc aggctgcacg cggtcgcaag ggcggttcta agtctaagcg cagcacagta
      481 gctacatctg cacgcacgct gaaaccgtgg gaggcattag ggatcagtcg cgcctggtat
      541 taccaactga aaaaacgagg tctcgtagag tagaccaaat aagcctatat cagataacag
      601 cgcctttctg gcgtcttttt gagcagcagg ttttttgccg tttttccccg ttcagccctg
      661 caatggcgcg gctttccgcg tgattgtggt ggtagtgctc gccgcagtct catgaccgag
      721 cgtagcgagc gaatgagcga ggaagcacca gggaatcgga acacggatgg aggcacttac
      781 gcgtctgggt ttggggatgt ctccagatcg tacttaagtt ccttaagtgc ttgtaatatc
      841 ctgtcttttt tatagcatag ctcgcctgtt tttgtgtcta ccagtcctgc ttcgatcagt
      901 agcccagccg cgagaaagag ccggtgattg cgttcccgtc gttgtttttt tccctcgatt
      961 cgtttttgga ccgcctgttt tcgttcgctc tcctgtgcct gtaagtcgtt tttttccgct
     1021 gttgccagta cgtggagaag tttgcgttcc tgttctgtga gtccggcttc gttgtcgatc
     1081 aggtcaataa tctgtctctg tcgtgcggtc tgttctccga gcttcctgat gaatccgatc
     1141 cgtttcttca gccattcgat gttgctgatg cgcatgttcg tgatgctccc tggtgttttt
     1201 ttgcggtgtg ttgtggtcgt gcagtatatc gcttgcggtc ggcgggtgca gggggtatct
     1261 tccgtgctaa ttcgtaaaat tagaaggaag cgcacttata tgtttctctc cttcgtcgag
     1321 aaacagtgcg ctcctgcaga gggctgaaac cgtggcaacc tatcacctca gcgttaaatt
     1381 tggtggcaag gggcaggctg caaaccacgc cgattacatc gagcgcaaag agaaataccg
     1441 cgaccgtcag gatttggaat attccgcaca cggaaacatg ccggaatggg ccagggataa
     1501 tccttcccat ttctggcagg cggctgacca gtttgaacgt gcgaacggtt cgacctatag
     1561 ggaacttgaa attgcattac cccgtgaact gacaccggaa cagcgccttg aactggtgca
     1621 ggattttgta cgtcaggaag ctggcgaacg gcacgcctgg agttttgcca ttcacaatcc
     1681 gaaagcgagc attgacggcg gcgaacagcc tcatgcgcat atcatgatga gtcagcgtgt
     1741 gaatgatggc attgaacgta caccggaaca gtatttccgg cgctataacg ccaggtatcc
     1801 cgagcgggga ggtgcgaaaa aggattccgg aagcctgact ctcactcagc aaaaagagca
     1861 gttgcgggaa ttgcgaaaac gctgggaggt gaagcacaac gaacatatga gaaagcatgg
     1921 ctttgaaagg ggcttcattg actgccggac gctgaaagaa cagggcattg aacgacgtcc
     1981 cgaagtgcat ctgggttttg aagctgccgg acggcttgat gatgcccagc gtcatgacat
     2041 catgcaaaaa cggtctgaac cggactattc ccgccatctt gaacaggcgc gaacggtcgg
     2101 gcatgacatc agggaatggc tggacactcc ggttaagcaa catgaactgg tcagaaggct
     2161 gcaacatgat gttgcgcggg agcatggctt tgacatgaga gacagagtga gcagacagaa
     2221 ccgaactgca cctgaacggg ttaaatcacc gtcacgtgga ttttctatgg gattctgaac
     2281 atggccagca cagaagaaaa attactcacg ctcctgttat cggtggagca tctgcaacat
     2341 accgcaatgg agcaacagaa agctgttgct gattctctgg ctgtggcaag ggaaactctt
     2401 cgcggatatc gggatatttc tgacggtgtg gcaaaacagg tcagggatga agtcagaaat
     2461 gaggtgcaga agctggacgt ggcaggactg ataggtgagc gcatcagcca ggattttcag
     2521 cggctggaag agtcagtcgc aaacatgaag gcaaacgcgg atgccctgaa cagggatatt
     2581 gcgacaattc acagcaatat cgtgcaggaa tacaacaccc ttaaagggta tagctggaaa
     2641 tggctgggtg gggtgtcagt ggcttttctg gttatgttct gtgtgctcgg atggggaatg
     2701 aaaacaatac tcgacaggaa ctacaacgcc acagcggcgg tgtatcagaa agttgtgaca
     2761 ctggaagata aactggtgga gaaacctgtc acggggaaga gccggaaaaa ctgacgcgct
     2821 tcgcttgtcc aacgcctcac gacccgcgaa aggatttcgc gaaccgttca ccgttttttt
     2881 caatatgatt ctgtctgctt tggtcgataa agcagacgat agtattccag ccttcgtata
     2941 tataacggta cgtgctcatc agtaagagct ggcggtattg tgttatcagg gggaagtttt
     3001 ttcagatact cacgaaatgc ctggcttgat gccataaaat ctacagtagc atcgataata
     3061 tgtggggtgt tttcacctaa ttttgccatg atgataatcc ttatattatc acggtttgtg
     3121 aatgataggg tgtcactatt atttatgcgt tagtgaacca gaaaaaatcc cccgttggca
     3181 tagggggata tgtaaggaga aataacttcg caggtgttct tgtttgtata acgtgaaaag
     3241 actgcacgca acttaataag tctgccgtac ttactttcag ttttcaacat aaggtttata
     3301 acaatgtatg gaatggaagt gagctttaag gtttcagctc taaaaaataa aatatttatt
     3361 agttggctgg ttgttgttct tgttctgtat gtatataaac actatcgaca tattatgacc
     3421 gggaacattc cttaccccct ctgcgctcgc agaggttttt tttgtcatta attaataatg
     3481 aactgaaatg cagttccgat acctccattt gcacattatc cctgacatat ggtaccggaa
     3541 ccgcattaat tttatgttat aagagtttat ttttaatgtc cagatggttt aatagtattt
     3601 atatgtaatt agctgtatgg atagtactgg ttttattgta taacttgtcg ttttttttta
     3661 ttgtttttgt gtggtgtgct aatctttaag ttataaaaaa accccttgga atacaggggc
     3721 aaaagtaagg aaaacaaatg gatgctcagt ttgtgtgaca ctttaaacta atggaactat
     3781 agagtggagg ttgttacagt cgcttttata aaacagcaac cagttttttt caatagtctg
     3841 atgatggttg tgacagtttt gtgatattgt tcaaaatgtt tcttgttgaa tgtttgtttt
     3901 ttggtctatt ggtgtctggt tttatttttt ctgctattac atatcaagaa atgtgattgg
     3961 tgtagcaaga gtggaatgcg atgtggataa actaattggt tgtcgaatct aatttactta
     4021 gaaaactaca cttgtttttg tgtatgaatt tagtgaattt gtctgtctat gtgttttcgt
     4081 gcatttataa atccttcgtc aggatcttaa atttagtgtt gaatatgtag ggcctgtacg
     4141 gtaattatta tttagcgtag ctacttcatc gttgttcgaa cacaaccaac aacctgtaat
     4201 tgttgtttct cttggttccc cacttttgtg gggtttttta tctcttccgt gcatcgtaaa
     4261 gcgcggactc cttctggaaa ggttgtttta gggcatcact gaggcatttt ttgcgtttgt
     4321 gaatattgag catccgataa ctaaaatatt tcccatagac tctgattctt gccaccgacc
     4381 ccaggtcggt tttttttgtc ctggcgagat gtgaagtttg gaaacaggaa accccgtcag
     4441 agcgccacgc accaggggag aaaacttgtc gttttgagcc gttcggtgtt cagaacgcac
     4501 gaaaccgatc gcacatcgct ttcgtgcata gttatgcaac gccatgaaaa cgattctgac
     4561 gcgtttttct ggtttggcct ggtgttttct tgtctttttg cgttttttgc gccagaaagc
     4621 gactgagggc gttttaaggg gggcgtacaa cgggagttat ggtaaatgga tcgggttttc
     4681 gggaaggttc gacaggattt gccgttgggt gtagtgtaag cgactgaaaa acaaacgccc
     4741 ctgaaatcat gtccagatcc ggcaagattc aacatgaaat acagagggcg tgtaggtcgc
     4801 ataacccgga gagaatcgta acacatgagc gccgcgcttc aatacttcga cgaaaattta
     4861 ccccatcgtc cctatcacac ggatgatctc gcttttggtc ttcgcatctc cggcaaaggg
     4921 cgtgcgcttc ttgcgcggta catacagcag aaccagcctc atgcgcagtt ctggctggtt
     4981 tttgacgttg accgcgaggg ggctgcgatt gactggagcg accggaacgc tcccgcgccg
     5041 aacatcaccg ttaaaaatcc tgtgaacgga cacgctcacc tgctctacgc actcaacatc
     5101 gccgtgagaa ccgcgcctga tgcctcggtt aaggcgctga aatatgccgc agcggttgaa
     5161 cgttcgctgt gtgagaaact ttgtgcggat gtgaactaca gcggcctgat ttgcaaaaat
     5221 ccgttccacc tggaatggct ggtgatggag tggcgcgagg aagcctatac cctcgatgaa
     5281 ctggctgatt atctcgattt gagcgcctca gagcgtcgta gcatcgataa acattacggg
     5341 atggggcgaa actgccacct gttcgaaatg acgcgcaaat gggcttacag ggcgattcgt
     5401 cagggctggc cagcgttctc acagtggctt gaagcggtga tccagcgtgt cgaaatgtac
     5461 aatgcatcgc ttcccgttcc gctttcactg gctgaatgtc gggctattgg caagagcatt
     5521 gcgaaataca cgcacaggaa cttcacgccg gaaactttcg cacagtatgt ggctgatacg
     5581 cacacgccag aaattcaggc tgcacgcggt cgcaagggcg gttctaagtc taagcgcagc
     5641 acagtagcta catctgcacg cacgctgaaa ccgtgggagg cattagggat cagtcgcgcc
     5701 tggtattacc aactgaaaaa acgaggtctc gtagagtaga ccaaataagc ctatatcaga
     5761 taacagcgcc tttctggcgt ctttttgagc agcaggtttt ttgccgtttt tccccgttca
     5821 gccctgcaat ggcgcggctt tccgcgtgat tgtggtggta gtgctcgccg cagtctcatg
     5881 accgagcgta gcgagcgaat gagcgaggaa gcaccaggga atcggaacac ggatggaggc
     5941 acttacgcgt ctgggtttgg ggatgtctcc agatcgtact taagttcctt aagtgcttgt
     6001 aatatcctgt cttttttata gcatagctcg cctgtttttg tgtctaccag tcctgcttcg
     6061 atcagtagcc cagccgcgag aaagagccgg tgattgcgtt cccgtcgttg tttttttccc
     6121 tcgattcgtt tttggaccgc ctgttttcgt tcgctctcct gtgcctgtaa gtcgtttttt
     6181 tccgctgttg ccagtacgtg gagaagtttg cgttcctgtt ctgtgagtcc ggcttcgttg
     6241 tcgatcaggt caataatctg tctctgtcgt gcggtctgtt ctccgagctt cctgatgaat
     6301 ccgatccgtt tcttcagcca ttcgatgttg ctgatgcgca tgttcgtgat gctccctggt
     6361 gtttttttgc ggtgtgttgt ggtcgtgcag tatatcgctt gcggtcggcg ggtgcagggg
     6421 gtatcttccg tgctaattcg taaaattaga aggaagcgca cttatatgtt tctctccttc
     6481 gtcgagaaac agtgcgctcc tgcagagggc tgaaaccgtg gcaacctatc acctcagcgt
     6541 taaatttggt ggcaaggggc aggctgcaaa ccacgccgat tacatcgagc gcaaagagaa
     6601 ataccgcgac cgtcaggatt tggaatattc cgcacacgga aacatgccgg aatgggccag
     6661 ggataatcct tcccatttct ggcaggcggc tgaccagttt gaacgtgcga acggttcgac
     6721 ctatagggaa cttgaaattg cattaccccg tgaactgaca ccggaacagc gccttgaact
     6781 ggtgcaggat tttgtacgtc aggaagctgg cgaacggcac gcctggagtt ttgccattca
     6841 caatccgaaa gcgagcattg acggcggcga acagcctcat gcgcatatca tgatgagtca
     6901 gcgtgtgaat gatggcattg aacgtacacc ggaacagtat ttccggcgct ataacgccag
     6961 gtatcccgag cggggaggtg cgaaaaagga ttccggaagc ctgactctca ctcagcaaaa
     7021 agagcagttg cgggaattgc gaaaacgctg ggaggtgaag cacaacgaac atatgagaaa
     7081 gcatggcttt gaaaggggct tcattgactg ccggacgctg aaagaacagg gcattgaacg
     7141 acgtcccgaa gtgcatctgg gttttgaagc tgccggacgg cttgatgatg cccagcgtca
     7201 tgacatcatg caaaaacggt ctgaaccgga ctattcccgc catcttgaac aggcgcgaac
     7261 ggtcgggcat gacatcaggg aatggctgga cactccggtt aagcaacatg aactggtcag
     7321 aaggctgcaa catgatgttg cgcgggagca tggctttgac atgagagaca gagtgagcag
     7381 acagaaccga actgcacctg aacgggttaa atcaccgtca cgtggatttt ctatgggatt
     7441 ctgaacatgg ccagcacaga agaaaaatta ctcacgctcc tgttatcggt ggagcatctg
     7501 caacataccg caatggagca acagaaagct gttgctgatt ctctggctgt ggcaagggaa
     7561 actcttcgcg gatatcggga tatttctgac ggtgtggcaa aacaggtcag ggatgaagtc
     7621 agaaatgagg tgcagaagct ggacgtggca ggactgatag gtgagcgcat cagccaggat
     7681 tttcagcggc tggaagagtc agtcgcaaac atgaaggcaa acgcggatgc cctgaacagg
     7741 gatattgcga caattcacag caatatcgtg caggaataca acacccttaa agggtatagc
     7801 tggaaatggc tgggtggggt gtcagtggct tttctggtta tgttctgtgt gctcggatgg
     7861 ggaatgaaaa caatactcga caggaactac aacgccacag cggcggtgta tcagaaagtt
     7921 gtgacactgg aagataaact ggtggagaaa cctgtcacgg ggaagagccg gaaaaactga
     7981 cgcgcttcgc ttgtccaacg cctcacgacc cgcgaaagga tttcgcgaac cgttcaccgt
     8041 ttttttcaat atgattctgt ctgctttggt cgataaagca gacgatagta ttccagcctt
     8101 cgtatatata acggtacgtg ctcatcagta agagctggcg gtattgtgtt atcaggggga
     8161 agttttttca gatactcacg aaatgcctgg cttgatgcca taaaatctac agtagcatcg
     8221 ataatatgtg gggtgttttc acctaatttt gccatgatga taatccttat attatcacgg
     8281 tttgtgaatg atagggtgtc actattattt atgcgttagt gaaccagaaa aaatcccccg
     8341 ttggcatagg gggatatgta aggagaaata acttcgcagg tgttcttgtt tgtataacgt
     8401 gaaaagactg cacgcaactt aataagtctg ccgtacttac tttcagtttt caacataagg
     8461 tttataacaa tgtatggaat ggaagtgagc tttaaggttt cagctctaaa aaataaaata
     8521 tttattagtt ggctggttgt tgttcttgtt ctgtatgtat ataaacacta tcgacatatt
     8581 atgaccggga acattcctta ccccctctgc gctcgcagag gttttttttg tcattaatta
     8641 ataatgaact gaaatgcagt tccgatacct ccatttgcac attatccctg acatatggta
     8701 ccggaaccgc attaatttta tgttataaga gtttattttt aatgtccaga tggtttaata
     8761 gtatttatat gtaattagct gtatggatag tactggtttt attgtataac ttgtcgtttt
     8821 tttttattgt ttttgtgtgg tgtgctaatc tttaagttat aaaaaaaccc cttggaatac
     8881 aggggcaaaa gtaaggaaaa caaatggatg ctcagtttgt gtgacacttt aaactaatgg
     8941 aactatagag tggaggttgt tacagtcgct tttataaaac agcaaccagt ttttttcaat
     9001 agtctgatga tggttgtgac agttttgtga tattgttcaa aatgtttctt gttgaatgtt
     9061 tgttttttgg tctattggtg tctggtttta ttttttctgc tattacatat caagaaatgt
     9121 gattggtgta gcaagagtgg aatgcgatgt ggataaacta attggttgtc gaatctaatt
     9181 tacttagaaa actacacttg tttttgtgta tgaatttagt gaatttgtct gtctatgtgt
     9241 tttcgtgcat ttataaatcc ttcgtcagga tcttaaattt agtgttgaat atgtagggcc
     9301 tgtacggtaa ttattattta gcgtagctac ttcatcgttg ttcgaacaca accaacaacc
     9361 tgtaattgtt gtttctcttg gttccccact tttgtggggt tttttatctc ttccgtgcat
     9421 cgtaaagcgc ggactccttc tggaaaggtt gttttagggc atcactgagg cattttttgc
     9481 gtttgtgaat attgagcatc cgataactaa aatatttccc atagactctg attcttgcca
     9541 ccga
//
