LOCUS       NZ_CP139815             3842 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Escherichia coli strain FLI21 plasmid pFLI21_6.
ACCESSION   NZ_CP139815
VERSION     NZ_CP139815.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38611544
            Assembly: GCF_034422715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-OCT-2020
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 476.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 05:08:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,128
            CDSs (total)                      :: 5,010
            Genes (coding)                    :: 4,649
            CDSs (with protein)               :: 4,649
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 156 of 361
            Pseudo Genes (incomplete)         :: 195 of 361
            Pseudo Genes (internal stop)      :: 44 of 361
            Pseudo Genes (multiple problems)  :: 35 of 361
            Pseudo Genes (short protein)      :: 4 of 361
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139815.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FLI21"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFLI21_6"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            complement(653..1366)
                     /locus_tag="U0532_RS25630"
                     /old_locus_tag="U0532_25630"
     CDS             complement(653..1366)
                     /locus_tag="U0532_RS25630"
                     /old_locus_tag="U0532_25630"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_135778405.1"
                     /translation="MADYIKSNILSQSYVHVEPEWLNSVSDSEKRKAIVEIHNKVSSFS
                     EERIRFFLDDDVHIEIKFEDGSIRAWVTTYGPVLLLIGSTITNYSSFRESIKHLVSDSQ
                     RVADIINAEVLFQSKSRSKKEVLRVESRKGVIGSIDRINNKIDAIEGRLKRGDCSPTLI
                     NEDLLDLNKLILELFSNIKDDDDAKLIAMALHDGIASLNIKKGRFKIKDSVDQYMYKSL
                     SDEKKLILKNLGSHK"
                     /name="hypothetical protein"
     gene            complement(1507..1827)
                     /locus_tag="U0532_RS25635"
                     /old_locus_tag="U0532_25635"
     CDS             complement(1507..1827)
                     /locus_tag="U0532_RS25635"
                     /old_locus_tag="U0532_25635"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_097344119.1"
                     /translation="MHTQHDVFIDAIHTKKKLRIRFYSEEDGGYIERVCAPMDYAAGKR
                     IHDGIPRYWVWDFESDKANHSLPLRSERIEYMRNEGEDFDPASFVTWTTAWVIGRNWES
                     YS"
                     /name="hypothetical protein"
     Typing          32..460
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.336"
                     /coverage="97.0"
                     /evalue="3.2599999999999995e-89"
     Typing          3112..3540
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.336"
                     /coverage="97.0"
                     /evalue="3.2599999999999995e-89"
ORIGIN
        1 taaagcgatg aagggcgatg gaagagagat aaaaaaggcc gcggcgttgc cggttttcca
       61 taggcttcgc cctcctgatg agcatcccag aaatcaggtg atttccggcg ctcaaatcag
      121 aagtggcgaa acccgacagg acttaaagat ccccactgtt tccggcaggt agctccctcg
      181 tgcgctctcc tgttcccacc ttacagatta cccacatcac tccgctgtta ttggccgcgt
      241 tttttcattc cacgcctgat gctgaggtct ggttaggtgg ttcgctccaa gctgggctgt
      301 atgcacgaac tccccgttca gcccgactac cacgtcttgt ccaggtaact gtactcttga
      361 atccaaccct gaaagacacg acaaatcgcc attggcggta gccaaaggta actggtttac
      421 ggagcggtaa agtacattca agtacacaca gagttcttga agtgctggcc agactacagc
      481 tacactggaa ggacagattt ggttactgcg ctccgttaaa gccagttacc tcggttaaaa
      541 aatctgccag gattttctaa ccttcgaaaa aaccacctgc cagggtggtt ttttcgttta
      601 ccgaaggtta cgctttcctc ttcgctaaag tcaataagct gagacaatgc agctacttat
      661 gcgaacctaa gtttttcaaa attaattttt tttcgtcgga gagagactta tacatatact
      721 gatcaacgga atcctttatt ttaaacctcc ccttctttat attaaggctt gcaatcccgt
      781 catgtaatgc catagctata agcttggcat catcatcatc tttaatattt gaaaacaact
      841 ccagtataag tttattcaaa tccaatagat cttcatttat taaagttggc gagcaatcac
      901 ctcttttcag cctcccctct atggcatcta ttttgttatt aattctatct atagagccaa
      961 tgacaccctt gcgtgattca actcgcaaaa cctctttctt acttcttgat tttgactgaa
     1021 aaagcacttc cgcattaata atatctgcaa ctctctgaga gtcagaaacc aagtgcttga
     1081 tactttccct aaaactactg taattggtga tcgttgatcc aattaatagt aaaacaggcc
     1141 cgtacgttgt aacccatgct ctaatagatc catcttcaaa tttaatctct atgtgaacat
     1201 catcatcgag aaaaaaccta attctttctt ctgaaaaaga actgacttta ttatgaattt
     1261 caacgattgc tttacgcttt tcactatcgg acactgaatt cagccattca ggttctacgt
     1321 gaacatatga ctgactaaga atattacttt taatataatc cgccataact ccgtccttga
     1381 cattaaatta aaatctcagg aactatccag tctaaaaacc cataaaaatc atacttataa
     1441 atccgaaatt tttcatgcaa agaggccact tcttgacgcc tccctcctct tcagaaaaaa
     1501 gctaactcaa ctataactct cccagtttct gccaataacc catgcagtag tccaagttac
     1561 gaaagatgct gggtcgaaat cctcgccttc attgcgcata tactcaatac gctcacttct
     1621 taatggcaaa gaatggttag ctttatcact ctcaaaatcc caaacccagt aacgagggat
     1681 cccatcatgt atgcgctttc cggcagcata atccattgga gcacatactc gctcgatata
     1741 accgccatct tcttcagaat aaaatcggat tctcaatttt tttttagtat gtattgcatc
     1801 aataaaaaca tcatgctgag tgtgcatagt catttccttg tatggttttc acagaggtaa
     1861 aaccactaaa aaatacgcta aacatgtagt atttaccata acatcccttg cccgcaccgc
     1921 tgaaaacgcc tcagagccat tttgaggcga acgggccgtt tccggcacct ttcccagcac
     1981 caacccctga aaaacgcgcc tgatgatttc tgtgcacctg catgactatg cacgcaggtg
     2041 aatggcgcgc ggtttcaggc ggatatcgta accggtgggg gattttcggc agtaaacgtg
     2101 gcgaagttcg ttggttaatc tgtacctgat tcaccgctta aaacctcttc caaaacaact
     2161 tttggtctga ctaaaaccgg tcaaaacccg tgttgaacgg tatgcatcgg agaagagacg
     2221 tagcacttgt aaatgtgtac cgcaacgtca cgggtgcagt gggggggggg ggggggattt
     2281 tcggcagtaa acgtggcgaa gttcgttggt taatctgtac ctgattcacc gcttaaaacc
     2341 tcttccaaaa caacttttgg tctgactaaa accggtcaaa acccgtgttg aacggtatgc
     2401 atcggagaag agacgtagca cttggtaaat gtgtacctgc ttaacgcttc acgggtacgc
     2461 agtttactgt gtgcccgtga agcgttggcc aggccgtcag gcatcgcagg atttattgcc
     2521 aagggggcgg atttaacgtg gatcaatcag cacgcagtga tgaacgggat tcggaagggg
     2581 cacccttgcg gcgcggcgat gtatacatcg ctaacgtgcc ttggttattt atggccaaaa
     2641 ggaacaggta gcagtctgat agcaaaaaag tagcaaaaag atagcaaaag gagagcaacc
     2701 gaaagcctca aaggctcaag agatagcaaa aaaatagcaa aagggtagca aatcaagcga
     2761 tgtcgggaga aacctgacca agagtctttg acgtaccggc ggtactccaa agacacatct
     2821 tgtcccgctg tgttgtaaca ctggagaact ggctgtgagc acaaaacatt tcctgtccgc
     2881 tgctttaact tttgtaatcc aagaacgtca gatgacgtca ctttgcggcg ttcgccctat
     2941 cctgcatcgt attaaaaaat gcagctatga cgtgtacggt gccgaacata agctcggaga
     3001 tattccgctt cctcgctcac tgactcgcta cgctcggtcg ttcggctgcg gcgagcggtg
     3061 ggggcagtca tatcaaagga taaagcgatg aagggcgatg gaagagagat aaaaaaggcc
     3121 gcggcgttgc cggttttcca taggcttcgc cctcctgatg agcatcccag aaatcaggtg
     3181 atttccggcg ctcaaatcag aagtggcgaa acccgacagg acttaaagat ccccactgtt
     3241 tccggcaggt agctccctcg tgcgctctcc tgttcccacc ttacagatta cccacatcac
     3301 tccgctgtta ttggccgcgt tttttcattc cacgcctgat gctgaggtct ggttaggtgg
     3361 ttcgctccaa gctgggctgt atgcacgaac tccccgttca gcccgactac cacgtcttgt
     3421 ccaggtaact gtactcttga atccaaccct gaaagacacg acaaatcgcc attggcggta
     3481 gccaaaggta actggtttac ggagcggtaa agtacattca agtacacaca gagttcttga
     3541 agtgctggcc agactacagc tacactggaa ggacagattt ggttactgcg ctccgttaaa
     3601 gccagttacc tcggttaaaa aatctgccag gattttctaa ccttcgaaaa aaccacctgc
     3661 cagggtggtt ttttcgttta ccgaaggtta cgctttcctc ttcgctaaag tcaataagct
     3721 gagacaatgc agctacttat gcgaacctaa gtttttcaaa attaattttt tttcgtcgga
     3781 gagagactta tacatatact gatcaacgga atcctttatt ttaaacctcc ccttctttat
     3841 at
//
