LOCUS       NZ_CP139814             1823 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Escherichia coli strain FLI21 plasmid pFLI21_5.
ACCESSION   NZ_CP139814
VERSION     NZ_CP139814.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38611544
            Assembly: GCF_034422715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-OCT-2020
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 476.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 05:08:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,128
            CDSs (total)                      :: 5,010
            Genes (coding)                    :: 4,649
            CDSs (with protein)               :: 4,649
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 156 of 361
            Pseudo Genes (incomplete)         :: 195 of 361
            Pseudo Genes (internal stop)      :: 44 of 361
            Pseudo Genes (multiple problems)  :: 35 of 361
            Pseudo Genes (short protein)      :: 4 of 361
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139814.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FLI21"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFLI21_5"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            complement(303..779)
                     /locus_tag="U0532_RS25625"
                     /old_locus_tag="U0532_25625"
     CDS             complement(303..779)
                     /locus_tag="U0532_RS25625"
                     /old_locus_tag="U0532_25625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          859..967
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(1170..1262)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.426"
                     /coverage="100.0"
                     /evalue="4.39e-26"
     Typing          1365..1625
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.893"
                     /coverage="100.0"
                     /evalue="8.5e-108"
ORIGIN
        1 ggctttcagg tcgccaggaa ccgcgccagc cccctgcaaa gcccatttgc acggtacagg
       61 cagccactcg gttggtaggt ggtcgtagac cgtcggtgtt acagcgatgg gggtctgggg
      121 ttctccccag acggtttccg gttgcgctca aggcgctgcc ggaaccgtgg cggctcggtc
      181 gttctggaga tcggggagca aaaacacgca agggattttg aggcggcaaa agagcaatgc
      241 aggatagaca atcatggtca gaacgttaac acgtagcccc tgattgtccg cctgccgctg
      301 gctcaatttg aaaacatggc catgattcgg cgctgaatat caccgtcttt gttgaccagt
      361 gttttagtga caaatgctgc atggtttttc gtagtgttgt atcccttcca gacttcatgt
      421 ggatcgaggg catacgcaca aaccttacca gcaacgggga ttctaactaa tgcatttttt
      481 gattctaagt aattgagcca acgaataatt gtgcttctgc taactccaag tttttcagct
      541 aggaattgct gatccgcaac gacagcgcca cacgtaggat cgatatgctc cgctaaaaat
      601 gagtacagct tcagtgctga tatgccttgg ctatccttca gcagctctcg aacgcgttcc
      661 caaccttttg gggatacctg tgtaaacctt gaattcttct ttgcttcttc gagatcctcg
      721 gtgtttttct ttgcctcatc tgaaagttgt ttcagtcgtt tttctgaatc aataaacatg
      781 attttttccc tctaaaaatc ccatgcacta acgttggttc aaatctaagc catcatgaca
      841 catctgtgca acaccttgtt gcgtgttgtc gcatccagga tgcgacaacg ttgtgtcacc
      901 ctggatgcga caggtaaaca aaaaacacac taaaaaaaca acaacttaca aatcggcgta
      961 aaagatctat tctaggcaag ggtgttgcgc gcaacgcaac gccctttggg ggtgtcgggg
     1021 cgaagccctg acccagtcac gtagcgatag cggagtgtat actggcttac tcctctgcaa
     1081 gcctcgccag ccgtgatttt tgccagattt cgtggttttc acgttcaaca gctcctgcac
     1141 gttttttgcc gtccgttcac cgttaacccc cgcagaaatc gggacaggat gtgcaaatgt
     1201 aataccgtca cccgcgacga tattccgttt gccatctggt cagggcttcg ccccgacacc
     1261 ccgtatcagt gcgtacgtgc tcaatgtggt ttccggttct cttccgcttc ctcgcccact
     1321 cgctcactaa cgttcggtcg ctggggctgc ggcgagcggt gccacctctc gcatgttgtc
     1381 ggtgattccg ctgttgttct ttcggggatg agtcgtcaga tagcccttca aacgcgctca
     1441 gaagctctca gacgcacgag gcggggttta aatgcccgtc agggcatgga aggcgcgaaa
     1501 aacgcttaca gagcgtttca ggaggtcagg cgcgccgata cccctagcca ggggctttca
     1561 ggtcgccagg aaccgcgcca gccccctgca aagcccattt gcacggtaca ggcagccact
     1621 cggttggtag gtggtcgtag accgtcggtg ttacagcgat gggggtctgg ggttctcccc
     1681 agacggtttc cggttgcgct caaggcgctg ccggaaccgt ggcggctcgg tcgttctgga
     1741 gatcggggag caaaaacacg caagggattt tgaggcggca aaagagcaat gcaggataga
     1801 caatcatggt cagaacgtta aca
//
