LOCUS       NZ_CP139809             4800 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Escherichia coli strain FLI21 plasmid pFLI21_8.
ACCESSION   NZ_CP139809
VERSION     NZ_CP139809.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38611544
            Assembly: GCF_034422715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 02-OCT-2020
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 476.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 05:08:59
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,128
            CDSs (total)                      :: 5,010
            Genes (coding)                    :: 4,649
            CDSs (with protein)               :: 4,649
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 361
            CDSs (without protein)            :: 361
            Pseudo Genes (ambiguous residues) :: 0 of 361
            Pseudo Genes (frameshifted)       :: 156 of 361
            Pseudo Genes (incomplete)         :: 195 of 361
            Pseudo Genes (internal stop)      :: 44 of 361
            Pseudo Genes (multiple problems)  :: 35 of 361
            Pseudo Genes (short protein)      :: 4 of 361
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139809.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FLI21"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFLI21_8"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            complement(<1..164)
                     /locus_tag="U0532_RS23015"
                     /old_locus_tag="U0532_23015"
                     /pseudo=""
     CDS             complement(<1..164)
                     /locus_tag="U0532_RS23015"
                     /old_locus_tag="U0532_23015"
                     /inference="COORDINATES: protein motif:HMM:NF016769.3"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /name="relaxase"
     gene            complement(149..634)
                     /locus_tag="U0532_RS23020"
                     /old_locus_tag="U0532_23020"
     CDS             complement(149..634)
                     /locus_tag="U0532_RS23020"
                     /old_locus_tag="U0532_23020"
                     /inference="COORDINATES: protein motif:HMM:NF016710.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_006674366.1"
                     /translation="MSEILNLAKGFEEKSKQQAQDIEQSVKREFGRLEMSIGAELQSSS
                     QSISNAIREQNGRLTGMIRASVSTAMAWIFTSFALLIGILGGIIWFQGTIITSRLSEMD
                     SYSQQLASLKAKSGAGVTIYSDDSRRNTYLVVLPKQAHGVETYTSQAGNTVVKYTAK"
                     /name="MbeB family mobilization protein"
     gene            complement(<681..1103)
                     /locus_tag="U0532_RS23025"
                     /old_locus_tag="U0532_23025"
                     /pseudo=""
     CDS             complement(<681..1103)
                     /locus_tag="U0532_RS23025"
                     /old_locus_tag="U0532_23025"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1312..1638)
                     /gene="mobC"
                     /locus_tag="U0532_RS23030"
                     /old_locus_tag="U0532_23030"
     CDS             complement(1312..1638)
                     /gene="mobC"
                     /locus_tag="U0532_RS23030"
                     /old_locus_tag="U0532_23030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_006674374.1"
                     /translation="MEKRTKEIKIRLTETEHQLLLERCDRTHLAEWLRHLGLGEHTSRK
                     RRVPSVAPELLRQVSGIGNNLNQIARRLNQSDSLTPSERVSLLAVLNSLDRQLGDLLEQ
                     NRDR"
                     /name="mobC"
     gene            complement(1879..3024)
                     /locus_tag="U0532_RS23035"
                     /old_locus_tag="U0532_23035"
     CDS             complement(1879..3024)
                     /locus_tag="U0532_RS23035"
                     /old_locus_tag="U0532_23035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kiwa anti-phage protein KwaB-like
                     domain-containing protein"
                     /protein_id="WP_006674372.1"
                     /translation="MAEEKLDLSELHHDAQKASELSSYYSHFDTKVEDFDLHVYFNFRH
                     KIKGGDGKYRYEYRVAKIDDTTVLKESIKDNVITKTLNDLSSGGVAVSDIKQLSDRAST
                     ISFLEDAKSYKDEQMSFLTGIIFDKGFGGDGDIPVVNFEKFDKISKVDSIVFVLRKGDK
                     CITIYKKQYPIQNLTKQKNHYFSYSKDTLSKIDADMISIDGKASIVFVKGTAYIYDQDV
                     FEKFFGYKKWIKKVAGEFVSEMKQKFSSIVDFGKVTDRLEKESADADAFSRKLARIYNN
                     KDLRASFNTVTPEKISDFLVENEYFARLLKYKSAEEPLEVSTHDQQEAFILIVSEGVLR
                     SLITGFDYLSPAAKRKIATGQTAQKPSSVVTRIPSSASDAA"
                     /name="Kiwa anti-phage protein KwaB-like domain-containing
                     protein"
     gene            complement(3017..3673)
                     /gene="kwaA"
                     /locus_tag="U0532_RS23040"
                     /old_locus_tag="U0532_23040"
     CDS             complement(3017..3673)
                     /gene="kwaA"
                     /locus_tag="U0532_RS23040"
                     /old_locus_tag="U0532_23040"
                     /inference="COORDINATES: protein motif:HMM:NF041622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-phage protein KwaA"
                     /protein_id="WP_024140105.1"
                     /translation="MKIKMYVLSLFFLFIPIIFYFSGIDDVWNVFTKANGFCDGVSNRI
                     VCITGNMGNQLSLSVSRLCVCIFLVFLCGYTYWWYKRQLSFLDRGITSAEVVVVGCEKE
                     STEYLSFLSTYIMPLVFTDLTKPSNVFNFIFILVIVGFLHIKTRRIHSNPTLSLFGVSA
                     YKIDYIIRVDGQDSDITKGLIVLSKDEISVKSVIRIIKKEDYITFAKYIRNDSNG"
                     /name="kwaA"
     gene            complement(3663..4559)
                     /locus_tag="U0532_RS23045"
                     /old_locus_tag="U0532_23045"
     CDS             complement(3663..4559)
                     /locus_tag="U0532_RS23045"
                     /old_locus_tag="U0532_23045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006674370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_006674370.1"
                     /translation="MSNAALELFNERLPHKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRAGAAIDWSDRNAPAPTLTITNPENGHAHLLYALKTSIRTAPDGKMK
                     PLRYAAAVENALRKKLEADAGYSGLICKNPNHRHWKIAVWQPELYTLDWLADFLDLNAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYGQWMLACVERATAYNMQFSAPLD
                     EKEVISIAKSISKWTYSKFTQQSFDEYVKKTHSAECQSIRGRKSSGGGRPKIRSEEWVS
                     LGISRATWYRKHYKNEN"
                     /name="replication initiation protein"
     Typing          1313..1505
                     /name="Col(Ye4449)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.443"
                     /coverage="100.0"
                     /evalue="8.63e-37"
     Typing          complement(4..1322)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.091"
                     /coverage="86.0"
                     /evalue="0.0"
ORIGIN
        1 agctggcctg ccatgcctgc agctgttgct cgtagcttgt ctgtaagctc tctaaggcgc
       61 tcagaagctg tttttccatc tcggtcatgg ttttggtcgt cctcggtgtt aaaaacgcgc
      121 tgaggcgttt cagggcgttc tgatggggtt atttcgccgt gtatttcact acagtgtttc
      181 cggcctgtga cgtgtaggtt tccacaccgt gtgcctgctt cggcagcacc accaggtagg
      241 tgttccggcg ggagtcgtcg ctgtaaatcg tcacacccgc gccacttttc gctttcaggc
      301 tcgccagctg ctggctgtag ctgtccattt cgctcaggcg tgaggtgatg atcgtgccct
      361 gaaaccagat tatcccgccc aggatgccga tcagcagggc gaagctggtg aatatccacg
      421 ccatcgccgt tgagacggag gctctgatca tgcccgtcag cctgccgttc tgctcgcgga
      481 tagcgttgct gatgctctgc gagcttgatt gcagttccgc gccgatagac atctccagcc
      541 tcccgaactc gcgcttcacg ctctgctcga tatcctgcgc ctgctgcttc gatttctcct
      601 cgaagccctt cgccaaattt aaaatctcgc tcataaatca ttcctttcag ccggatattt
      661 ctgccaccgt ccggatcggc aatgctgatg ctgcttttcg tttcccgcgc cacggtcagg
      721 ccgtagcttt ccagctgcgc caccacgtcc tgccggctgc ggatcacacc ctgctgcatc
      781 aggttcatca ggccggtggt gattttctct gccgccagct gtttgtcgcg gggcagatcg
      841 ctggcctgtg tcagcggtcg gcgacaggtc gggtcgttcg ggtcacgcag ccccagccgg
      901 tcattagtca gggtctgcca ggcgttgacg cgggggcggt cggcgcggtc aaagtagggc
      961 tgaaggcgtt tgccgctctg caattcgatg ttcgggatga caaaattcag ctcaagccgt
     1021 cccttgtcgc ggtgctccac ccacagacag gcgtactggt cacggtcgag gccggtcagc
     1081 agcgtgtgct cccattcatc catcaggcgc gatttctcgg catccgcaat gtccggctcc
     1141 tggaatgaca gcacaccgga agtgtaggcg cgggaaaagt cgcagccgtc gataagctcg
     1201 cgcacatatt cagggttgcc gcgcagcaca ctggcctgtt cgcgttgccg gtcttttccc
     1261 agcagataat ccaccggacc actgcctgaa cccgcaccgc gcgagtgaat tttaacgatc
     1321 acggttctgc tccagtaaat cgccaagctg ccgatcgaga ctgttcagca cggcaagcag
     1381 cgagacacgt tcagagggcg taagggaatc actctggttc agacggcggg cgatctggtt
     1441 caggttgtta ccaatcccgc tgacctgtcg cagcagttcg ggcgccacac tgggcacacg
     1501 gcgcttgcgt gaggtgtgct cgcccagacc aaggtgacgc agccactcag caagatgagt
     1561 ccggtcgcag cgctcaagca gtagctgatg ctcggtttcg gtgagccgga ttttgatctc
     1621 tttcgttcgc ttttccatag gctccgctaa gaaaaaattc gcaccaccgt gagaattttt
     1681 cgcagtggat gccgggggtt tcggggggcg gcgagccccc tgaaccagtc acagacggca
     1741 cctcgcagag gggacgctgt gtgtactggc ttatggtatg gtgtagtatg tcagattctg
     1801 tgctggctgc aaaaaaaaca gccctctcta agagagggct gttatctgat ataggttgtt
     1861 ttgtctcaag ctcgttcgtt aggcggcgtc agatgcgctg gagggtatcc tagttactac
     1921 agaagaaggt ttttgcgcgg tttggcctgt cgcaattttt cttttcgcgg cagggcttaa
     1981 ataatcaaaa ccagtaataa gcgagcgaag aacgccctca gaaactatta atataaatgc
     2041 ttcctgctga tcatgggttg atacttctaa aggttcttcc gctgatttgt actttagcaa
     2101 tctagcgaaa tactcatttt caactaaaaa atcactgatt ttttctggtg taacagtatt
     2161 aaaggaagct cttagatctt tattgttata tattcttgcc agtttccttg aaaaggcatc
     2221 tgcatcagca ctttcttttt caagcctgtc ggtaactttt ccaaagtcta ctatcgatga
     2281 aaatttttgc ttcatttcag aaacaaattc gcccgcaact ttttttatcc acttcttgta
     2341 tccgaaaaat ttctcaaaaa catcctgatc atagatatat gcagtacctt taacaaaaac
     2401 aatagaggct tttccatcaa ttgatatcat gtcagcatct atttttgata acgtatcttt
     2461 gctatatgag aagtaatgat ttttctgttt ggtcaggttc tgtataggat actgtttttt
     2521 gtatattgtt atacatttat cacctttcct taagacaaaa acaattgagt caacctttga
     2581 tattttatca aacttctcaa aattgacgac aggaatatcc ccatcacctc caaatccctt
     2641 atcaaaaata atacctgtca aaaaagacat ctgttcatct ttgtatgact ttgcatcttc
     2701 taaaaatgat atagtgctag ccctatcact aagctgcttt atatcagata ctgcaactcc
     2761 accgctactt agatcattaa gggtttttgt tatcacgtta tctttgattg attctttcag
     2821 aacagtagta tcatctattt ttgcaacccg atattcgtaa cggtactttc catcaccgcc
     2881 ctttatctta tgcctaaagt taaaatatac atgaaggtca aaatcttcta cctttgtatc
     2941 gaagtgggag taataggatg agagctcaga ggccttctgc gcgtcatgat ggagctcgct
     3001 taaatccagt ttttcttcag ccatttgaat cgttccttat gtattttgcg aatgtaatgt
     3061 agtcttcttt ttttatgatt cgaattacac ttttcacaga tatctcatcc ttcgacagga
     3121 caatcaatcc ttttgtgata tcagaatcct gaccatcaac cctgattata tagtcaattt
     3181 tgtaagcaga aacaccaaac aatgaaagcg ttgggttgct atgaatcctt cttgttttta
     3241 tatgaagaaa tccgacaata actagaataa aaatgaaatt gaaaacattt gaaggttttg
     3301 ttaagtctgt aaaaaccagg ggcattatgt aagttgacaa aaatgacaga tactcagttg
     3361 actctttttc gcatccaaca acaaccactt cggcagaggt aatcccccta tccaaaaatg
     3421 aaagctgtct cttataccac cagtaagtat agccacacaa aaagaccaaa aaaatacaaa
     3481 cgcataacct tgacacggat agagatagct ggttgcccat attacctgtt atacagacaa
     3541 tacgattact gacgccatca caaaaaccat tagctttggt aaaaacgttc cagacatcat
     3601 caataccaga aaaataaaaa attattggta taaacaaaaa aaacaaagac aagacataca
     3661 tcttaatttt catttttata gtgcttccta taccacgtag cgcgactaat acctagagag
     3721 acccactcct cagatcttat ttttggccgc cctccgccag agctcttgcg ccctcttata
     3781 ctctgacatt cagctgagtg tgttttctta acatattcat cgaaagattg ctgtgtgaat
     3841 ttagaatatg tccatttaga tatactcttc gctatactta tgacctcctt ttcatctaat
     3901 ggtgctgaaa actgcatgtt gtaagctgta gctctttcaa cacaagctag catccattgt
     3961 ccatactcag gccagccctg acgaatagca cgataagccc attttcgggt tttgtcgaac
     4021 aaggtgcagt tacggcctaa tccgtagtcg gcaacgattt ctttatcgtt cgcagcgttc
     4081 aggtcaagga aatcggccag ccagtccagt gtgtagagtt ccggctgcca cacggcaatt
     4141 ttccagtgcc tgtggttcgg atttttgcag attaaccctg aataccccgc gtcagcctct
     4201 aattttttac gtagcgcgtt ctccacggcg gcagcgtagc gcagcggctt catttttccg
     4261 tccggcgctg tgcgtatcga ggtttttagc gcgtacagta gatgcgcgtg tccgttttcg
     4321 gggttggtga tggtcagtgt gggcgcggga gcattgcggt cactccagtc aatagcggca
     4381 ccggcgcgat caacatcgaa tcccagccag aacatggcat gaggctgatt gaactggatg
     4441 tatttcgcca gaatggcgcg ttccttgccg gcaatgcgta caccaaagtg caaatcgtcc
     4501 gaaaagtacg gcttgtgcgg caagcgttcg ttgaatagtt cgagtgcggc gttactcaaa
     4561 gcgccctgcg cttatggcgg gctgttgtct ggcacagttc atgtgctaat atcctttcaa
     4621 ggttttgtac ctggccctgg tagtcatgct catccgccaa gatagctcat gattgccggg
     4681 gctttgtttt ttctgccgtt aaacttatac cttaaaacaa cacgttgtca ctaatcacta
     4741 aagagaaacg ggtttccaac agaaatctct tacgataatc acgttgttac cgtagataat
//
