LOCUS       NZ_CP139802            10036 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Escherichia coli strain Nord8 plasmid pNORD8_4.
ACCESSION   NZ_CP139802
VERSION     NZ_CP139802.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38610607
            Assembly: GCF_034421795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10036)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-OCT-2018
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 04:42:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 168 of 324
            Pseudo Genes (incomplete)         :: 180 of 324
            Pseudo Genes (internal stop)      :: 45 of 324
            Pseudo Genes (multiple problems)  :: 60 of 324
            Pseudo Genes (short protein)      :: 3 of 324
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139802.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Nord8"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pNORD8_4"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            complement(<1..126)
                     /locus_tag="U0546_RS27465"
                     /old_locus_tag="U0546_27465"
                     /pseudo=""
     CDS             complement(<1..126)
                     /locus_tag="U0546_RS27465"
                     /old_locus_tag="U0546_27465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /name="mobilization protein"
     gene            301..699
                     /locus_tag="U0546_RS27470"
                     /old_locus_tag="U0546_27470"
     CDS             301..699
                     /locus_tag="U0546_RS27470"
                     /old_locus_tag="U0546_27470"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(903..1817)
                     /locus_tag="U0546_RS27475"
                     /old_locus_tag="U0546_27475"
     CDS             complement(903..1817)
                     /locus_tag="U0546_RS27475"
                     /old_locus_tag="U0546_27475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            3554..3763
                     /locus_tag="U0546_RS27480"
                     /old_locus_tag="U0546_27480"
     CDS             3554..3763
                     /locus_tag="U0546_RS27480"
                     /old_locus_tag="U0546_27480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(3829..4362)
                     /locus_tag="U0546_RS27485"
                     /old_locus_tag="U0546_27485"
     CDS             complement(3829..4362)
                     /locus_tag="U0546_RS27485"
                     /old_locus_tag="U0546_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(4365..5291)
                     /locus_tag="U0546_RS27490"
                     /old_locus_tag="U0546_27490"
     CDS             complement(4365..5291)
                     /locus_tag="U0546_RS27490"
                     /old_locus_tag="U0546_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_001331297.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTAPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            5466..5864
                     /locus_tag="U0546_RS27495"
                     /old_locus_tag="U0546_27495"
     CDS             5466..5864
                     /locus_tag="U0546_RS27495"
                     /old_locus_tag="U0546_27495"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222914.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKELKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            complement(6068..6982)
                     /locus_tag="U0546_RS27500"
                     /old_locus_tag="U0546_27500"
     CDS             complement(6068..6982)
                     /locus_tag="U0546_RS27500"
                     /old_locus_tag="U0546_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271348.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVATSARTL
                     KPWEALGISRAWYYQLKKRGLVE"
                     /name="replication initiation protein"
     gene            8719..8928
                     /locus_tag="U0546_RS27505"
                     /old_locus_tag="U0546_27505"
     CDS             8719..8928
                     /locus_tag="U0546_RS27505"
                     /old_locus_tag="U0546_27505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001096196.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTDE
                     HVPLYIRRLEYYRLLYRPKQTESY"
                     /name="hypothetical protein"
     gene            complement(8994..9527)
                     /locus_tag="U0546_RS27510"
                     /old_locus_tag="U0546_27510"
     CDS             complement(8994..9527)
                     /locus_tag="U0546_RS27510"
                     /old_locus_tag="U0546_27510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168770.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVTLED
                     KLVEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            complement(9530..>10036)
                     /locus_tag="U0546_RS27515"
                     /old_locus_tag="U0546_27515"
     CDS             complement(9530..>10036)
                     /locus_tag="U0546_RS27515"
                     /old_locus_tag="U0546_27515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_225385638.1"
                     /translation="YFRRYNARYPERGGAKKDSGSLTLTQQKEQLRELRKRWEVKHNEH
                     MRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRLDDAQRHDIMQKRSEPDYSRHLE
                     QARTVGHDIREWLDTPVKQHELVRRLQHDVAREHGFDMRDRVSRQNRTAPERVKSPSRG
                     FSMGF"
                     /name="MobA/MobL family protein"
     Typing          complement(1043..1817)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(6208..6982)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.778"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          complement(4369..5291)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.052"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(9534..10036)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.024"
                     /coverage="100.0"
                     /evalue="6.23e-103"
ORIGIN
        1 gtttccgtgt gcggaatatt ccaaatcctg acggtcgcgg tatttctctt tgcgctcgat
       61 gtaatcggcg tggtttgcag cctgcccctt gccaccaaat ttaacgctga ggtgataggt
      121 tgccacggtt tcagccctct gcaggagcgc actgtttctc gacgaaggag agaaacatat
      181 aagtgcgctt ccttaatttt acgaattagc acggaagata ccccctgcac ccgccgaccg
      241 caagcgatat actgcacgac cacaacacac cgcaaaaaaa caccagggag catcacgaac
      301 atgcgcatca gcaacatcga atggctgaag aaacggatcg gattcatcag gaagctcgga
      361 gaacagaccg cacgacagag acagattatt gacctgatcg acaacgaagc cggactcaca
      421 gaacaggaac gcaaacttct ccacgtactg gcaacagcgg aaaaaaacga cttacaggca
      481 caggagagcg aacgaaaaca ggcggtccaa aaacgaatcg agggaaaaaa acaacgacgg
      541 gaacgcaatc accggctctt tctcgcggct gggctactga tcgaagcagg actggtagac
      601 acaaaaacag gcgagctatg ctataaaaaa gacaggatat tacaagcact taaggaactt
      661 aagtacgatc tggagacatc cccaaaccca gacgcgtaag tgcctccatc cgtgttccga
      721 ttccctggtg cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagca
      781 ctaccaccac aatcacgcgg aaagccgcgc cattgcaggg ctgaacgggg aaaaacggca
      841 aaaaacctgc tgctcaaaaa gacgccagaa aggcgctgtt atctgatata ggcttatttg
      901 gtctactcta cgagacctcg ttttttcagt tggtaatacc aggcgcgact gatccctaat
      961 gcctcccacg gtttcagcgt gcgtgcagat gtagctactg tgctgcgctt agacttagaa
     1021 ccgcccttgc gaccgcgtgc agcctgaatt tctggcgtgt gcgtatcagc cacatactgt
     1081 gcgaaagttt ccggcgtgaa gttcctgtgc gtgtatttcg caatgctctt gccaatagcc
     1141 cgacattcag ccagtgaaag cggaacggga agcgatgcat tgtacatttc gacacgctgg
     1201 atcaccgctt caagccactg tgagaacgct ggccagccct gacgaatcgc cctgtaagcc
     1261 catttgcgcg tcatttcgaa caggtggcag tttcgcccca tcccgtaatg tttatcgatg
     1321 ctacgacgct ctgaggcgct caaatcgaga taatcagcca gttcatcgag ggtataggct
     1381 tcctcgcgcc actccatcac cagccattcc aggtggaacg gatttttgca aatcaggccg
     1441 ctgtagttca catccgcaca aagtttctca cacagcgaac gttcaaccgc tgcggcatat
     1501 ttcagcgcct taaccgaggc atcaggcgcg gttctcacgg cgatgttgag tgcgtagagc
     1561 aggtgagcgt gtccgttcac aggattttta acggtgatgt tcggcgcggg agcgttccgg
     1621 tcgctccagt caatcgcagc cccctcgcgg tcaacgtcaa aaaccagcca gaactgcgca
     1681 tgaggctggt tctgctgtat gtaccgcgca agaagcgcac gccctttgcc ggagatgcga
     1741 agaccaaaag cgagatcatc cgtgtgatag ggacgatggg gtaaattttc gtcgaagtat
     1801 tgaagcgcgg cgctcatgtg ttacgattct ctccgggtta tgcgacctac acgccctctg
     1861 tatttcatgt tgaatcttgc cggatctgga catgatttca ggggcgtttg tttttcagtc
     1921 gcttacacta cacccaacgg caaatcctgt cgaaccttcc cgaaaacccg atccatttac
     1981 cataactccc gttgtacgcc ccccttaaaa cgccctcagt cgctttctgg cgcaaaaaac
     2041 gcaaaaagac aagaaaacac caggccaaac cagaaaaacg cgtcagaatc gttttcaggg
     2101 cgttgcataa ctatgcacga aagcgatgtg cgatcggttt cgtgcgttct gaacaccgaa
     2161 cggctcaaaa cgacaagttt tctcccctgg tgcgtggcgc tctgacgggg tttcctgttt
     2221 ccaaacttca catctcgcca ggacaaaaaa aaaccgacct ggggtcggtg gcaagaatca
     2281 gagtctatgg gaaatatttt agttatcgga tgctcaatat tcacaaacgc aaaaaatgcc
     2341 tcagtgatgc cctaaaacaa cctttccaga aggagtccgc gctttacgat gcacggaaga
     2401 gataaaaaac cccacaaaag tggggaacca agagaaacaa caattacagg ttgttggttg
     2461 tgttcgaaca acgatgaagt agctacgcta aataataatt accgtacagg ccctacatat
     2521 tcaacactaa atttaagatc ctgacgaagg atttataaat gcacgaaaac acatagacag
     2581 acaaattcac taaattcata cacaaaaaca agtgtagttt tctaagtaaa ttagattcga
     2641 caaccaatta gtttatccac atcgcattcc actcttgcta caccaatcac atttcttgat
     2701 atgtaatagc agaaaaaata aaaccagaca ccaatagacc aaaaaacaaa cattcaacaa
     2761 gaaacatttt gaacaatatc acaaaactgt cacaaccatc atcagactat tgaaaaaaac
     2821 tggttgctgt tttataaaag cgactgtaac aacctccact ctatagttcc attagtttaa
     2881 agtgtcacac aaactgagca tccatttgtt ttccttactt ttgcccctgt attccaaggg
     2941 gtttttttat aacttaaaga ttagcacacc acacaaaaac aataaaaaaa aacgacaagt
     3001 tatacaataa aaccagtact atccatacag ctaattacat ataaatacta ttaaaccatc
     3061 tggacattaa aaataaactc ttataacata aaattaatgc ggttccggta ccatatgtca
     3121 gggataatgt gcaaatggag gtatcggaac tgcatttcag ttcattatta attaatgaca
     3181 aaaaaaacct ctgcgagcgc agagggggta aggaatgttc ccggtcataa tatgtcgata
     3241 gtgtttatat acatacagaa caagaacaac aaccagccaa ctaataaata ttttattttt
     3301 tagagctgaa accttaaagc tcacttccat tccatacatt gttataaacc ttatgttgaa
     3361 aactgaaagt aagtacggca gacttattaa gttgcgtgca gtcttttcac gttatacaaa
     3421 caagaacacc tgcgaagtta tttctcctta catatccccc tatgccaacg ggggattttt
     3481 tctggttcac taacgcataa ataatagtga caccctatca ttcacaaacc gtgataatat
     3541 aaggattatc atcatggcaa aattaggtga aaacacccca catattatcg atgctactgt
     3601 agattttatg gcatcaagcc aggcatttcg tgagtatctg aaaaaacttc cccctgataa
     3661 cacaataccg ccagctctta ctgatgagca cgtaccgtta tatatacgaa ggctggaata
     3721 ctatcgtctg ctttatcgac caaagcagac agaatcatat tgaaaaaaac ggtgaacggt
     3781 tcgcgaaatt ctttcgcggg tcgtgaggcg ttggacaagc gaagcgcgtc agtttttccg
     3841 gctcttcccc gtgacaggtt tctccaccag tttatcttcc agtgtcacaa ctttctgata
     3901 caccgccgct gtggcgttgt agttcctgtc gagtattgtt ttcattcccc atccgagcac
     3961 acagaacata accagaaaag ccactgacac cccacccagc catttccagc tatacccttt
     4021 aagggtgttg tattcctgca cgatattgct gtgaattgtc gcaatatccc tgttcagggc
     4081 atccgcgttt gccttcatgt ttgcgactga ctcttccagc cgctgaaaat cctggctgat
     4141 gcgctcacct atcagtcctg ccacgtccag cttctgcacc tcatttctga cttcatccct
     4201 gacctgtttt gccacaccgt cagaaatatc ccgatatccg cgaagagttt cccttgccac
     4261 agccagagaa tcagcaacag ctttctgttg ctccattgcg gtatgttgca gatgctccac
     4321 cgataacagg agcgtaagta atttttcttc tgtgctggcc atgttcagaa tcccatagaa
     4381 aatccacgtg acggtgattt aacccgttca ggtgcagttc ggttctgtct gctcactctg
     4441 tctctcatgt caaagccatg ctcccgcgca acatcatgtt gcagccttct gaccagttca
     4501 tgttgcttaa ccggagtgtc cagccattcc ctgatgtcat gcccgaccgt tcgcgcctgt
     4561 tcaagatggc gggaatagtc cggttcagac cgtttttgca tgatgtcatg acgctgggca
     4621 tcatcaagcc gtccggcagc ttcaaaaccc agatgcactt cgggacgtcg ttcaatgccc
     4681 tgttctttca gcgtccggca gtcaatgaag cccctttcaa agccatgctt tctcatatgt
     4741 tcgttgtgct tcacctccca gcgttttcgc aattcccgca actgctcttt ttgctgagtg
     4801 agagtcaggc ttccggaatc ctttttcgca cctccccgct cgggatacct ggcgttatag
     4861 cgccggaaat actgttccgg tgtacgttca atgccatcat tcacacgctg actcatcatg
     4921 atatgcgcat gaggctgttc gccgccgtca atgctcgctt tcggattgtg aatggcaaaa
     4981 ctccaggcgt gccgttcgcc agcttcctga cgtacaaaat cctgcaccag ttcaaggcgc
     5041 tgttccggtg tcagttcacg gggtaatgca atttcaagtt ccctataggt cgaaccgttc
     5101 gcacgttcaa actggtcagc cgcctgccag aaatgggaag gattatccct ggcccattcc
     5161 ggcatgtttc cgtgtgcgga atattccaaa tcctgacggt cgcggtattt ctctttgcgc
     5221 tcgatgtaat cggcgtggtt tgcagcctgc cccttgccac caaatttaac gctgaggtga
     5281 taggttgcca cggtttcagc cctctgcagg agcgcactgt ttctcgacga aggagagaaa
     5341 catataagtg cgcttcctta attttacgaa ttagcacgga agataccccc tgcacccgcc
     5401 gaccgcaagc gatatactgc acgaccacaa cacaccgcaa aaaaacacca gggagcatca
     5461 cgaacatgcg catcagcaac atcgaatggc tgaagaaacg gatcggattc atcaggaagc
     5521 tcggagaaca gaccgcacga cagagacaga ttattgacct gatcgacaac gaagccggac
     5581 tcacagaaca ggaacgcaaa cttctccacg tactggcaac agcggaaaaa aacgacttac
     5641 aggcacagga gagcgaacga aaacaggcgg tccaaaaacg aatcgaggga aaaaaacaac
     5701 gacgggaacg caatcaccgg ctctttctcg cggctgggct actgatcgaa gcaggactgg
     5761 tagacacaaa aacaggcgag ctatgctata aaaaagacag gatattacaa gcacttaagg
     5821 aacttaagta cgatctggag acatccccaa acccagacgc gtaagtgcct ccatccgtgt
     5881 tccgattccc tggtgcttcc tcgctcattc gctcgctacg ctcggtcatg agactgcggc
     5941 gagcactacc accacaatca cgcggaaagc cgcgccattg cagggctgaa cggggaaaaa
     6001 cggcaaaaaa cctgctgctc aaaaagacgc cagaaaggcg ctgttatctg atataggctt
     6061 atttggtcta ctctacgaga cctcgttttt tcagttggta ataccaggcg cgactgatcc
     6121 ctaatgcctc ccacggtttc agcgtgcgtg cagatgtagc tactgtgctg cgcttagact
     6181 tagaaccgcc cttgcgaccg cgtgcagcct gaatttctgg cgtgtgcgta tcagccacat
     6241 actgtgcgaa agtttccggc gtgaagttcc tgtgcgtgta tttcgcaatg ctcttgccaa
     6301 tagcccgaca ttcagccagt gaaagcggaa cgggaagcga tgcattgtac atttcgacac
     6361 gctggatcac cgcttcaagc cactgtgaga acgctggcca gccctgacga atcgccctgt
     6421 aagcccattt gcgcgtcatt tcgaacaggt ggcagtttcg ccccatcccg taatgtttat
     6481 cgatgctacg acgctctgag gcgctcaaat cgagataatc agccagttca tcgagggtat
     6541 aggcttcctc gcgccactcc atcaccagcc attccaggtg gaacggattt ttgcaaatca
     6601 ggccgctgta gttcacatcc gcacaaagtt tctcacacag cgaacgttca accgctgcgg
     6661 catatttcag cgccttaacc gaggcatcag gcgcggttct cacggcgatg ttgagtgcgt
     6721 agagcaggtg agcgtgtccg ttcacaggat ttttaacggt gatgttcggc gcgggagcgt
     6781 tccggtcgct ccagtcaatc gcagccccct cgcggtcaac gtcaaaaacc agccagaact
     6841 gcgcatgagg ctggttctgc tgtatgtacc gcgcaagaag cgcacgccct ttgccggaga
     6901 tgcgaagacc aaaagcgaga tcatccgtgt gatagggacg atggggtaaa ttttcgtcga
     6961 agtattgaag cgcggcgctc atgtgttacg attctctccg ggttatgcga cctacacgcc
     7021 ctctgtattt catgttgaat cttgccggat ctggacatga tttcaggggc gtttgttttt
     7081 cagtcgctta cactacaccc aacggcaaat cctgtcgaac cttcccgaaa acccgatcca
     7141 tttaccataa ctcccgttgt acgcccccct taaaacgccc tcagtcgctt tctggcgcaa
     7201 aaaacgcaaa aagacaagaa aacaccaggc caaaccagaa aaacgcgtca gaatcgtttt
     7261 cagggcgttg cataactatg cacgaaagcg atgtgcgatc ggtttcgtgc gttctgaaca
     7321 ccgaacggct caaaacgaca agttttctcc cctggtgcgt ggcgctctga cggggtttcc
     7381 tgtttccaaa cttcacatct cgccaggaca aaaaaaaacc gacctggggt cggtggcaag
     7441 aatcagagtc tatgggaaat attttagtta tcggatgctc aatattcaca aacgcaaaaa
     7501 atgcctcagt gatgccctaa aacaaccttt ccagaaggag tccgcgcttt acgatgcacg
     7561 gaagagataa aaaaccccac aaaagtgggg aaccaagaga aacaacaatt acaggttgtt
     7621 ggttgtgttc gaacaacgat gaagtagcta cgctaaataa taattaccgt acaggcccta
     7681 catattcaac actaaattta agatcctgac gaaggattta taaatgcacg aaaacacata
     7741 gacagacaaa ttcactaaat tcatacacaa aaacaagtgt agttttctaa gtaaattaga
     7801 ttcgacaacc aattagttta tccacatcgc attccactct tgctacacca atcacatttc
     7861 ttgatatgta atagcagaaa aaataaaacc agacaccaat agaccaaaaa acaaacattc
     7921 aacaagaaac attttgaaca atatcacaaa actgtcacaa ccatcatcag actattgaaa
     7981 aaaactggtt gctgttttat aaaagcgact gtaacaacct ccactctata gttccattag
     8041 tttaaagtgt cacacaaact gagcatccat ttgttttcct tacttttgcc cctgtattcc
     8101 aaggggtttt tttataactt aaagattagc acaccacaca aaaacaataa aaaaaaacga
     8161 caagttatac aataaaacca gtactatcca tacagctaat tacatataaa tactattaaa
     8221 ccatctggac attaaaaata aactcttata acataaaatt aatgcggttc cggtaccata
     8281 tgtcagggat aatgtgcaaa tggaggtatc ggaactgcat ttcagttcat tattaattaa
     8341 tgacaaaaaa aacctctgcg agcgcagagg gggtaaggaa tgttcccggt cataatatgt
     8401 cgatagtgtt tatatacata cagaacaaga acaacaacca gccaactaat aaatatttta
     8461 ttttttagag ctgaaacctt aaagctcact tccattccat acattgttat aaaccttatg
     8521 ttgaaaactg aaagtaagta cggcagactt attaagttgc gtgcagtctt ttcacgttat
     8581 acaaacaaga acacctgcga agttatttct ccttacatat ccccctatgc caacggggga
     8641 ttttttctgg ttcactaacg cataaataat agtgacaccc tatcattcac aaaccgtgat
     8701 aatataagga ttatcatcat ggcaaaatta ggtgaaaaca ccccacatat tatcgatgct
     8761 actgtagatt ttatggcatc aagccaggca tttcgtgagt atctgaaaaa acttccccct
     8821 gataacacaa taccgccagc tcttactgat gagcacgtac cgttatatat acgaaggctg
     8881 gaatactatc gtctgcttta tcgaccaaag cagacagaat catattgaaa aaaacggtga
     8941 acggttcgcg aaattctttc gcgggtcgtg aggcgttgga caagcgaagc gcgtcagttt
     9001 ttccggctct tccccgtgac aggtttctcc accagtttat cttccagtgt cacaactttc
     9061 tgatacaccg ccgctgtggc gttgtagttc ctgtcgagta ttgttttcat tccccatccg
     9121 agcacacaga acataaccag aaaagccact gacaccccac ccagccattt ccagctatac
     9181 cctttaaggg tgttgtattc ctgcacgata ttgctgtgaa ttgtcgcaat atccctgttc
     9241 agggcatccg cgtttgcctt catgtttgcg actgactctt ccagccgctg aaaatcctgg
     9301 ctgatgcgct cacctatcag tcctgccacg tccagcttct gcacctcatt tctgacttca
     9361 tccctgacct gttttgccac accgtcagaa atatcccgat atccgcgaag agtttccctt
     9421 gccacagcca gagaatcagc aacagctttc tgttgctcca ttgcggtatg ttgcagatgc
     9481 tccaccgata acaggagcgt aagtaatttt tcttctgtgc tggccatgtt cagaatccca
     9541 tagaaaatcc acgtgacggt gatttaaccc gttcaggtgc agttcggttc tgtctgctca
     9601 ctctgtctct catgtcaaag ccatgctccc gcgcaacatc atgttgcagc cttctgacca
     9661 gttcatgttg cttaaccgga gtgtccagcc attccctgat gtcatgcccg accgttcgcg
     9721 cctgttcaag atggcgggaa tagtccggtt cagaccgttt ttgcatgatg tcatgacgct
     9781 gggcatcatc aagccgtccg gcagcttcaa aacccagatg cacttcggga cgtcgttcaa
     9841 tgccctgttc tttcagcgtc cggcagtcaa tgaagcccct ttcaaagcca tgctttctca
     9901 tatgttcgtt gtgcttcacc tcccagcgtt ttcgcaattc ccgcaactgc tctttttgct
     9961 gagtgagagt caggcttccg gaatcctttt tcgcacctcc ccgctcggga tacctggcgt
    10021 tatagcgccg gaaata
//
