LOCUS       NZ_CP139801             6606 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Escherichia coli strain Nord8 plasmid pNORD8_7.
ACCESSION   NZ_CP139801
VERSION     NZ_CP139801.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38610607
            Assembly: GCF_034421795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6606)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-OCT-2018
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 04:42:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 168 of 324
            Pseudo Genes (incomplete)         :: 180 of 324
            Pseudo Genes (internal stop)      :: 45 of 324
            Pseudo Genes (multiple problems)  :: 60 of 324
            Pseudo Genes (short protein)      :: 3 of 324
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139801.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Nord8"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pNORD8_7"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            complement(<1..846)
                     /gene="mobQ"
                     /locus_tag="U0546_RS27435"
                     /old_locus_tag="U0546_27435"
     CDS             complement(<1..846)
                     /gene="mobQ"
                     /locus_tag="U0546_RS27435"
                     /old_locus_tag="U0546_27435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_302296060.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     A"
                     /name="mobQ"
     gene            1025..1279
                     /gene="mobC"
                     /locus_tag="U0546_RS27440"
                     /old_locus_tag="U0546_27440"
     CDS             1025..1279
                     /gene="mobC"
                     /locus_tag="U0546_RS27440"
                     /old_locus_tag="U0546_27440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091745.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            1821..2534
                     /locus_tag="U0546_RS27445"
                     /old_locus_tag="U0546_27445"
                     /pseudo=""
     CDS             1821..2534
                     /locus_tag="U0546_RS27445"
                     /old_locus_tag="U0546_27445"
                     /inference="COORDINATES: protein motif:HMM:NF013235.3"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2793..4952)
                     /gene="mobQ"
                     /locus_tag="U0546_RS27450"
                     /old_locus_tag="U0546_27450"
     CDS             complement(2793..4952)
                     /gene="mobQ"
                     /locus_tag="U0546_RS27450"
                     /old_locus_tag="U0546_27450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_322419622.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQTAFEKQWQQWGASGLAALRVQL
                     LKREEELRRKEQEDTERSRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFERVDLMRDRSGYICDRSEIGQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKG
                     LRYAGYGVAVNHDADSHISPAPELMEFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRREEDARTHATERRHDRGWSQ"
                     /name="mobQ"
     gene            5131..5385
                     /gene="mobC"
                     /locus_tag="U0546_RS27455"
                     /old_locus_tag="U0546_27455"
     CDS             5131..5385
                     /gene="mobC"
                     /locus_tag="U0546_RS27455"
                     /old_locus_tag="U0546_27455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091748.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKTEVTRQADVTRILPLINELEALPGQ"
                     /name="mobC"
     gene            complement(<5682..>6606)
                     /locus_tag="U0546_RS27460"
                     /old_locus_tag="U0546_27460"
                     /pseudo=""
     CDS             complement(<5682..>6606)
                     /locus_tag="U0546_RS27460"
                     /old_locus_tag="U0546_27460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029347.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /name="conjugal transfer protein"
     Typing          complement(489..847)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          848..1024
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          complement(4595..4953)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
     Typing          4954..5130
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          1822..2533
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.951"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2..933)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.678"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2797..5039)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.465"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5677..6533)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.951"
                     /coverage="100.0"
                     /evalue="5.83e-171"
ORIGIN
        1 cgcattactg tccttcggca acgcttcgat gatgtcacgt agtgtgtacg aagttgcgtt
       61 gtcaggaagc acgatattca gaaggtcagc cacacggcgg gcaggctctg tcagcccgct
      121 gtttctgagt tcggtacggg aaatctgtat ttccaggtta agtctgttaa tcagacgatt
      181 ctgttcgccg cgttctgttt cgataccgcg tgtttccatc gcacaggcag actttccgag
      241 gtgaatacca ggcgttttct caagcccctg tcgctcgtat gaacgatggt ctatctcttc
      301 ctgataaccg gcgttcgcca gtgccctgtt cgcatggtca gcccatgatg cacgccacgt
      361 ctcggcatgt gtccggtcgt tccagtccct gacctttcca ccgaatccag caggtccgac
      421 agctctcgtg gtcagcatga tgtgcgcatg gggatttgtt ttatccatgt gatggaacgc
      481 cacatcagca atcatgccct gactgacaaa gttttctctg acaaaggcga gaacggtttc
      541 ccgtgcggca tcctgcggta gttcgcgggg gatggcgagt tcaatttccc gcgcaagttg
      601 tgagttcttc cggcgttcgg ccttctcaac ggcattccag agttcggcac ggttcgtaca
      661 ccaggcaggg gctgtcgcgg gagtcagaat cacagcttca gccacaccgc ttttgcgggt
      721 gtagtcgtga gtcagtccgg tacgctcatc aagcatcagt gaaccggaac gataggcagc
      781 agaagcaacg gcgctgtagc cgctgtttct ggagatgatt ttcatgctga gatgataaat
      841 cgccatttct gccgtaatgc ctttttgtgg ttttctttca gccccgcagg gcgcacacaa
      901 tcgaaacgga gtggagattg tataagtgcg cacttcgtga ttttccgtgc attatgccag
      961 ctcagttttt aaactaccag tgacgcgcta aaatgcgatt tttcatcgcg cacggagtta
     1021 catcatgagc agaacactcg aacagaagat tgctgatgct gaggccagat tacagcgcct
     1081 gaaggcgaag agcaggagcc tggacacagc gcagaaggtc attgtgggtg cagcattgct
     1141 ggcaaaggtc agaaagccgg aggaggtgca gttgcgtgcc tggttactcc agttcctgaa
     1201 ggcagaggtg acacgacagg ctgatgtgac gcgcatactg ccgctgatta acgagctgga
     1261 agcgctgcca ggacagtgat tgtatcgtcc gctcgccgca gccccagcga ccgagcgtta
     1321 gcgagcgagt gggcgaggaa gcggaagtga atccgtcgcc tgattgagca catacgcacg
     1381 gtttttcggg aggtgatatg cgctgaaccc gcatgaatac tggatttcca cagggttgga
     1441 gggggtgtat tcggaacttg caattctgct tttttgcaac acattgtttt tattggtttt
     1501 ttgttttcag caccccaagt tatccacagg ctcgtcgtta agtttttaga tcttgttttt
     1561 aagatcttgt ttttaatact tgttttagcg ccaaaattca ttaatagaaa tcaatatgtt
     1621 acaacgctat aggtgatagg aatttaaggt aaaggtgata gaatttaagg tgcttgggtg
     1681 gaggtaattt aaggtaaagg tggaggagat ttaaggtaat attcacagga aaatgggggg
     1741 atgaaaaaaa aattgctcac caatgcttaa aggtgatagg cactttttag tttcacctca
     1801 tttttggccg ggtggttaaa atgacatcaa accctctgat agcctacaag tcgaatgcgc
     1861 tggttgaggc cagctacaag ctaaccttac aagaacagcg tttcttgttg ctgtgtatca
     1921 gccgatttaa ctctggtgct gacgtagctt cacctgaatt gcaaaagaca atgaccatta
     1981 ccgctgcgga atattttgat tcattccctg atatggggcg gaaaaatgcc gaggttcagc
     2041 tacaggaagc gatcgatcgt ttgtgggaca ggtcgatcat tctcaaggat gacgagaagc
     2101 gtgaggagtt ccgctggatc cagtatcgag cacagtacgc gaagggagag ggcaaggcac
     2161 aaatcacttt ttctgacgcg gtaatgcctt atctgacaca gttgaagggg caattcaccc
     2221 gcgtcgttat taaaaatatt tctgatttat caagtagtta cagtataaga atatacgaaa
     2281 ttctccagca attccgcagt acgggggaac gtattatcgc ccttaatgat tttaaatcga
     2341 tcactgatag ggcttgatgg taaatataaa catttcagaa acgttaaata gggatactaa
     2401 taaaaccctg tgttgacgaa ctgaacaaaa aaagcgacct ggctgtaacg gttgagacga
     2461 tcaaaaaggg gcgcacggtt gtagccctgc attttcgctt caaggaagac aaacagatca
     2521 agatgacgat ctaaaatcga ttttaaggca cctaaaacgc tccctgctgc gttttttttg
     2581 tgttagtagt gcgaaggtac tattgaagtg aaaaattgcg tcaaaaacgc tctgatgcag
     2641 cgaaaaaaga acccccaatt tttcgggggc ggaaaaacaa aactatgctt gcgttatgtg
     2701 agtgtgcttc ttgcattcat ccctgtcgat gaacattgaa gaacatcgac gaacataaca
     2761 cgatgttaaa caaaacaaaa tattgttcac attcactgtg accaaccgcg atcatgtctc
     2821 ctctcggtgg cgtgtgttcg tgcatcttcc tcgcgccgcc attcgtccca ggtacgattg
     2881 aaacccagcg agcggaacag atgatccggc tgtttttctg gtgtgttgac ggcagcaaaa
     2941 agcggtgcct cacgggtttt cagatgaaac tccattagtt ccggcgcagg tgatatgtgg
     3001 ctgtctgcat cgtgatttac ggcaacacca taccctgcat accgtaaccc cttatcggtc
     3061 agtgcactga tgaccagctg gctgttatcg gctgagggaa cggcaaacgc gggatacagc
     3121 gtaccattaa gccggacaac accgcccaga gagtcatcca cgcccatgac cagcaaaagc
     3181 tgaagctggc gggtttcccg gtcgacagaa ctcattaccg gcatcagcgt cggtggcaga
     3241 cgtccctgcg taaaatccac cttccttgct gccagtgatg gcatggctgg ctggcggtct
     3301 tctccaagac agaaagactc cggccatgtc ttcggctggc ggagattgcg ggtatattgc
     3361 ggccatgtct ggcaaacctg cgtcagaagt tgctcaaagg tcgcagagga ataaacacgt
     3421 tgcagtggtg gatgttgacc gatttcgctt cggtcacaga tataaccact cctgtcacgc
     3481 attaaatcaa cacgctcaaa gattctggtg cggtatttct ccagctcgtt cccccagagc
     3541 gtatattctc cgttccggtt gtgaagcaga aggtaacagg tcatttcccc gttaccaggc
     3601 atgggggcct cgccataccc cgtaacgact gcgctcagta cccctgaatc cataacggca
     3661 ttgtcatttt tcctgagctg ttccgctcgt cgctccgcgc gactacgttc tgtatcctcc
     3721 tgctccttcc ggcgcaattc ctcttcacgt ttaagcaact ggactctgag agctgcaagg
     3781 ccggatgccc cccactgctg ccactgtttt tcaaatgccg tctgactcgc gggaatgtcc
     3841 tgtttctcca ccttccggta atgctgtcgc aatttttcca tttgctggct cagcgactgg
     3901 atttttccct gttcgctacg gtattccggt gccgcccatt gcatcagggg aatacgggaa
     3961 aaaccggacg aaaacggcga tgattttttc aggctggcag catggctcat cgcttccttt
     4021 cgttcggtgt tcagctcttc ccagcgtctg cgcgtcgcct gcatatctgc catcatcgac
     4081 atggcattac tggtgagttt ccatgccgca ttactgtcct tcggcaacgc ttcgatgatg
     4141 tcacgtagtg tgtacgaagt tgcgttgtca ggaagcacga tattcagaag gtcagccaca
     4201 cggcgggcag gctctgtcag cccgctgttc ctgagttcag tgcgggaaat ctgtatttcc
     4261 aggttaagcc tgttaatcag acgattctgt tcgccgcgtt ctgtttcgat accgcgtgtt
     4321 tccatcgcac aggcagactt tccgaggtga ataccaggcg ttttctcaag cccctgtcgc
     4381 tcgtatgaac gatggtctat ctcttcctga taaccggcgt tcgccagtgc tctgtttgca
     4441 tggtcagccc atgatgcacg ccacgtctcg gcatgtgtcc ggtcgttcca gtccctgacc
     4501 tttccgccga acccagcagg tccgacagcc ctcgtggtca gcatgatgtg cgcatgggga
     4561 tttgttttat ccatgtgatg gaacgccaca tcagcaatca tgccctgact gacaaaattt
     4621 tctctgacaa aggcgagaac ggtttcccgt gcggcatcct gcggcagttc acgggggata
     4681 gcgagttcaa tttcccgcgc aagttgtgag ttcttccggc gttcggcctt ctcaacggca
     4741 ttccagagtt cggcacggtt cgtacaccaa gcaggggctg tcgcgggggt cagaatcaca
     4801 gcttcagcca caccgctttt gcgggtgtag tcgtgagtca gtccggtacg ctcatcaagc
     4861 atcagtgaac cggaacgata ggcagcggaa gctacggcgc tgtagccgct gcttctggag
     4921 ataattttca tgctgagatg ataaatcgcc atttctgccg taatgccttt ttgtagtttt
     4981 ctttcagccc cgcagggcgc acacaatcga aacggagtgg agattgtata agtgcgcact
     5041 tcgtgatttg tcgtgcatta tgccagctca gtttttaaac taccagtggc gcgctaaaat
     5101 gcgatttttc atcgcgcacg gagttacatc atgagcagaa cgctcgaaca gaagattgct
     5161 gatgctgagg cgagattaca gcgcctgaag gcgaagagcc ggagcctgga tacagcgcag
     5221 aaggttgttg tgggtgcagc attgctggca aaggtcagaa agccggagga ggtgcagttg
     5281 cgtgcctggt tactccagtt cctgaagaca gaggtgacac gacaggctga tgtgacgcgc
     5341 atactgccgc tgattaacga gctggaggcg ctgccaggac agtgattgta tcgtccgctc
     5401 gccgcagccc cagcgaccga gcgttagcga gcgagtgggc gaggaagcgg aagtgaatcc
     5461 gtcgccaggt tgagcacata cgcacggttt ttcggcaggt gatatgcgct gaacccgcat
     5521 gaatactgga tttccacagg gttggagggg gagtattcgg aacttgcaat tctgctttct
     5581 tgcaacacat tgtttttatt ggttttttgt ttttcaccaa ccctgcatac cgttaacccc
     5641 ttagatcttg tttttaagat ttgtttttaa tattgtttta gggtcagtgc actgatgacc
     5701 agctggctgt tatcggctga gggaacggca aacgcgggat acagcgtacc attaagccgg
     5761 acaacaccgc ccagagagtc atccacgccc atgaccagca aaagctgaag ctggcgggtt
     5821 tcccggtcga cagaactcat taccggcatc agcgtcgcta gcagacgtcc ctgcgtaaaa
     5881 tccaccttcc ttgctgccag tgatggcatg gctggctggc ggtcttctcc aagacagaaa
     5941 gactccggcc atgtcttcgg ctggcggaga ttgcgggtat attgcggcca tgtctggcaa
     6001 acctgcgtca gaagttgctc aaaggtcgca gaggaataaa cacgttgcag tggtggatgt
     6061 tgaccgattt cgcttcggtc acagatataa ccactcctgt cacgcattaa atcaacacgc
     6121 tcaaagattc tggtgcggta tttctccagc tcgttccccc agagcgtgta ttctccgttc
     6181 cggttgtgaa gcagaaggta acaggtcatt tccccgttac caggcatggg ggcctcgcca
     6241 tactccgtaa cgactgcgct cagtacccct gaatccataa cggcattgtc atttttcctg
     6301 agctgttccg ctcgtcgctc cgcgcgacta cgttctgtat cctcctgctc cttccggcgc
     6361 aattcctctt cacgtttaag caactggact ctgagagctg caaggccgga tgccccccac
     6421 tgctgccact gtttttcaaa tgccgtctga ctcgcgggaa tgtcctgttt ctccaccttc
     6481 cggtaatgct gtcgcaattt ttccatttgc tggctcagcg actggatttt ccctgttcgc
     6541 tacggtattc cggtgccgcc cattgcttac gggaaaaacc ggacgaaaac ggccgggaaa
     6601 tgcccg
//
