LOCUS       NZ_CP139799             5540 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Escherichia coli strain Nord8 plasmid pNORD8_5.
ACCESSION   NZ_CP139799
VERSION     NZ_CP139799.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38610607
            Assembly: GCF_034421795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5540)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5540)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-OCT-2018
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 04:42:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 168 of 324
            Pseudo Genes (incomplete)         :: 180 of 324
            Pseudo Genes (internal stop)      :: 45 of 324
            Pseudo Genes (multiple problems)  :: 60 of 324
            Pseudo Genes (short protein)      :: 3 of 324
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139799.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..5540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Nord8"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pNORD8_5"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            57..290
                     /locus_tag="U0546_RS26970"
                     /old_locus_tag="U0546_26970"
     CDS             57..290
                     /locus_tag="U0546_RS26970"
                     /old_locus_tag="U0546_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(837..1133)
                     /locus_tag="U0546_RS26975"
                     /old_locus_tag="U0546_26975"
     CDS             complement(837..1133)
                     /locus_tag="U0546_RS26975"
                     /old_locus_tag="U0546_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000047647.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLTQ
                     ADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKFLNGKKPEEGLNAWKGDLP"
                     /name="hypothetical protein"
     gene            1345..1542
                     /locus_tag="U0546_RS26980"
                     /old_locus_tag="U0546_26980"
     CDS             1345..1542
                     /locus_tag="U0546_RS26980"
                     /old_locus_tag="U0546_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001335786.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSVS
                     GLDVELIDVSCESEWEEHSH"
                     /name="hypothetical protein"
     gene            1560..1844
                     /locus_tag="U0546_RS26985"
                     /old_locus_tag="U0546_26985"
     CDS             1560..1844
                     /locus_tag="U0546_RS26985"
                     /old_locus_tag="U0546_26985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Z C-terminal domain-related protein"
                     /protein_id="WP_000625497.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIAF
                     IWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
                     /name="colicin Z C-terminal domain-related protein"
     gene            complement(1908..2297)
                     /locus_tag="U0546_RS26990"
                     /old_locus_tag="U0546_26990"
     CDS             complement(1908..2297)
                     /locus_tag="U0546_RS26990"
                     /old_locus_tag="U0546_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000477210.1"
                     /translation="MFKSSVKSITSSTILTLLIGCSSTETNKNIVYKVGGDSIILNKYC
                     ILKMYPSQSEKNKGVIIEVNTDSLCKKKLNEITSKNIGKPLISYFDGKVISQPAIIHSA
                     LNPTHYYQPVNDETTVMEIIKAYSK"
                     /name="hypothetical protein"
     gene            complement(2704..2817)
                     /locus_tag="U0546_RS26995"
                     /old_locus_tag="U0546_26995"
     CDS             complement(2704..2817)
                     /locus_tag="U0546_RS26995"
                     /old_locus_tag="U0546_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001427889.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGN"
                     /name="hypothetical protein"
     gene            complement(3485..3676)
                     /locus_tag="U0546_RS27000"
                     /old_locus_tag="U0546_27000"
     CDS             complement(3485..3676)
                     /locus_tag="U0546_RS27000"
                     /old_locus_tag="U0546_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3742..4065
                     /locus_tag="U0546_RS27005"
                     /old_locus_tag="U0546_27005"
     CDS             3742..4065
                     /locus_tag="U0546_RS27005"
                     /old_locus_tag="U0546_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000956005.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGTR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4055..4369
                     /locus_tag="U0546_RS27010"
                     /old_locus_tag="U0546_27010"
     CDS             4055..4369
                     /locus_tag="U0546_RS27010"
                     /old_locus_tag="U0546_27010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_224446634.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELID
                     ATPFAKKYTSGVLSFAEKELPPGGREKVMASFERVLMPGLEKISTASCGWSTRTRDGLS
                     "
                     /name="hypothetical protein"
     gene            4336..>4704
                     /locus_tag="U0546_RS27015"
                     /old_locus_tag="U0546_27015"
                     /pseudo=""
     CDS             4336..>4704
                     /locus_tag="U0546_RS27015"
                     /old_locus_tag="U0546_27015"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4742..5260
                     /locus_tag="U0546_RS27020"
                     /old_locus_tag="U0546_27020"
     CDS             4742..5260
                     /locus_tag="U0546_RS27020"
                     /old_locus_tag="U0546_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_023144181.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSC
                     GTLWYLGGMIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPHQ
                     GKDWVQLME"
                     /name="MbeB family mobilization protein"
     gene            5267..5500
                     /locus_tag="U0546_RS27025"
                     /old_locus_tag="U0546_27025"
     CDS             5267..5500
                     /locus_tag="U0546_RS27025"
                     /old_locus_tag="U0546_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_001555310.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMKLSQQVQHLSERTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          3313..3400
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(2746..3302)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3..394
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="85.496"
                     /coverage="100.0"
                     /evalue="2.76e-40"
     Typing          3710..4062
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.153"
                     /coverage="100.0"
                     /evalue="3.72e-70"
     Typing          4313..5532
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.538"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cagaggtgat tccccatcag gggaaggact gggtacaact gatggagtga cacagagtga
       61 cggagctgga aagacatttg ctgaacgcat tagagcaact gcaacaggac tatatgcagc
      121 ggctgaacga atgggagagc gccttcgtgg aattgcagaa aatgttttcg cttacgcaac
      181 gggacaacgc gatgctgaac gagcgggtca tgaagttgag tcagcaggtg cagcacttga
      241 gcgagcggac agaacgcttg agccagttat acagcgagaa caggagataa gggacgaacg
      301 gctgatacag gaacgcgaac atgagttatc cctggagcgt gagcgccagc tggaaataca
      361 ggaacggaca ctggatggcc cttcgctggg atggtaatca gagacaggct aaaatgttgt
      421 cagcgcgttt ccggtgcatt ttttgggata atggttggtt cggggcaccg ggaacgcgct
      481 tcagtcataa accttgctga aaacacagga acgcgttaaa acgcatacgg gcgcgattct
      541 ggtgtgtctt tcatagtcgt gtttttgcct ctattgccag atcgcgctca ttcccctgaa
      601 cttctgtttt cctgctcaaa tccccgtctt cttcgcttcc gaaagtatga aaaaaatccg
      661 tttcagcggt ctctgactgg caattcctgc gtgattcacc tggctgcata ggtatgcatg
      721 tggttaaaat ttaccgggtg cgatcgcggc agttttccgg gtggtttgtt gccattttta
      781 cctgtctgct gccgtgatcg caccgaacgc gtttcggcgg tgcgtacaat aagtgattat
      841 ggtaaatcac ccttccaggc gttcagccct tcttccggtt tttttccgtt caggaattta
      901 cgggcgagac gaatgcgtct tgcctggtaa atcgcatcgt aggcctgtga ctgtcccaca
      961 ccgttactga tttcactgac cagatggcgc aggtcggcct gtgtcagtgt gtaaacgtgt
     1021 ttctggatga ggtcacgggc tgtccgctct tcgtatccgg cagcgataag tgcagccagc
     1081 aggcgtttgt ctgttttgct cagtcggtgg cgaccagatt ttgttttgct cacgttatct
     1141 cctctctgtt ttttctcctg atgttatccg gtgctgtttt tctgctgcga atcttccctt
     1201 cctgattttt ataagggtga ttcagatcac aaaaatgaaa attcatatca ttgacaggaa
     1261 aaataatgcg gcgtaattta tactgtatat aaaactagta tttatatata cagtgtattc
     1321 tgtgtaaata aagggaggga taaaatgaaa gaagaaaaag tattgcttca ccgtttctta
     1381 tttgtagtta ggaataagaa tggttgtgaa ctatcatgtt cggcagacct tatgggaaca
     1441 agggatgatg tttataagta tttctctgat tctgttagtg ggctagatgt tgagcttata
     1501 gatgtttctt gcgagagtga atgggaagaa cattcgcatt gaactaatca ggagattata
     1561 tgatttctca aaatgtttgg gctcctcctt tcttttttgg tccttgggtt gaacttgaaa
     1621 atcatactgg tgatatcgcg tacactgtct attttgatac attgtctaat gtacccggtc
     1681 catttattgc ttttatctgg tatgccccta atgggccaag aacagttaac gttattggtc
     1741 cagcatctta tcagatctct tgcaagggcg ctggtgttga taaaattaaa tttaaaagtg
     1801 tcagtgggac agggcagatt attcatgtat acactacctt ataataagag ctgaataaat
     1861 ataatcctgc ctaagctata ccttaagcag gattatatct attaatatta ttttgaatat
     1921 gcttttatga tttccatcac tgttgtttca tcgttcacag gttgataata atgggttgga
     1981 tttagtgcag aatgaatgat tgcaggttgg gatattactt ttccatcaaa atatgaaatc
     2041 aggggtttcc ctatattttt actagttatt tcgtttagtt ttttcttaca taaactatct
     2101 gtgtttactt ctattatgac gcctttgttt ttttctgatt gggaagggta cattttaagt
     2161 atgcaatatt tattcagaat gatgctgtca ccaccaactt tgtaaacaat gtttttgttt
     2221 gtttcagtac ttgaacatcc tataagtaag gtcagtatag tactcgatgt tattgacttt
     2281 actgatgatt tgaacatatt aactccattt tattttgtta aaattccaat ctataaatac
     2341 atatacacgt aaaaataaac aatgcgaacg ttttcactta tattatcccc atgatgtgat
     2401 tgaatggtgg ttccatataa gagagggcaa ttatactatc tttctctcct gaactgatgg
     2461 gattgcagtt tagagaaaaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2521 taatctgctg cctgtaaacg aaaaaaccac cctggcaggt ggtttttcga aggttagcta
     2581 atcctggcag attacctaac cgggtagctg gcttaagtgg agcacagata ccaaaagatg
     2641 tcctttcagt gtagccgcta taaggcttca acttcaagag ttctgcgtat ctaaatccac
     2701 tttttagtta ccaatggctg ctgccagtgg cgttaagtcg tgtcttaccg ggttggactc
     2761 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     2821 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     2881 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaaacg gtaaggtcgg
     2941 aacaggagag cgcacgaggg agcttccggg gggaaacgcc tggtatcttt aagtcctgtc
     3001 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3061 ctatggaaaa acgccagcgg cgcggccttc ttcctgttcc tggtcttttg ctcacatgtt
     3121 ttttccggtg ttatcccctg attctgtgga taacagcatt tcgtttttga gtgagtagat
     3181 accgctcgcc gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaaaag
     3241 cgcctggacg tgcattttct ccttacgcat ctgtgcggca tttcacaccc ggtatggcgc
     3301 acgtttcata caatccgcac tgatgccgca tggttaagcc agtatacact ccgctatcgc
     3361 tacgtgactg gttcagggct gcgccccgaa acccgctaaa acaccctgac gcgccctggc
     3421 gggcttgtct gctcccggca tccgctcaca ggcaagctgt gaccgtctcc gggagctgca
     3481 tatgtcagag gttttcactg tcatccccga agcgcgcgag gcagctgcgg taaagctcat
     3541 cggcgtggtc gtgaagcgat tcacagatat ctgcctgttc atccgcgtcc agttcgttga
     3601 gcttctccag cagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt
     3661 tttcctgttt agtcactgat gcctccgtgt aaggggggat ttctgttcat gggggtaatg
     3721 ataccgatga aacgcgagag gatgctcacg atacgggtta ctgatgatga acatgcccgg
     3781 ttactggaac gctgtgaggg taaacaactg gcggtatgga tgcggcgggt ctgcctgggt
     3841 gagccggttg cccgttccgg gaggctgccg acactggcac cgccgttact gcgtcagctg
     3901 gcagccatcg ggaataacct gaaccagaca gcccgtaagg tgaacagcgg acagtggtct
     3961 tccggcgacc gggttcaggt ggtggccgca ctgatggcca tcggggatga gctgcgccgg
     4021 ttgcgtctgg ccgtcaggga acaggggacg cgggatgata gttaagtttc atgcccgggg
     4081 aaaaggtggt ggcagtggtc cggttgatta cctgctggga agggagcgta accgggaagg
     4141 ggcaacggtg cttcagggta atccggaaga agtacgggaa ctcatcgatg ccacgccatt
     4201 tgcgaagaaa tacacgtccg gtgtgctgtc gttcgcggag aaggagctgc caccgggagg
     4261 acgtgaaaag gtgatggcga gctttgagcg tgtactgatg cccggtctcg aaaaaatcag
     4321 tacagcatcc tgtgggtgga gcaccaggac aagggacggc ttgagctgaa ttttgtcatt
     4381 ccgaacatgg agctacagac cggaaaacgc ctccagccgt actacgaccg ggcagacagg
     4441 ccgagaattg atgcctggca gacgctggtg aatcaccatt acgggctgca tgaccccaac
     4501 gccccggaga accgccggat actgactctc tctgataacc tgccggaaac gaaacaggcg
     4561 cttgcagaga gcgtcacacg cgggatagac gctctttatc atgtcgggga gataaaaggc
     4621 cgtcaggatg tgattcaggc gctcacagag gcagggcttg aagtggtcag ggtgacgcga
     4681 agcagtatca gcattgcaga cccgaacggt gggaagaata tcaggctgaa aggagcattt
     4741 tatgagcaat cttttacaga cgggcgcgga gttcgagaaa aagctgaaag agagagccga
     4801 atctacagag acaatactga acgacgagtt caggaagctc gaaaaatctg taagcaaggc
     4861 tgtgacatca aacgagacga aaatcaaaga cgctatagcc ctgttcacag cttcgacaga
     4921 ggaatcactg aaaaagcacc gggaaggggt gaaagaggcg atgatgcagc acaggaagga
     4981 cgtgttaaag ctggcaggga atacgggcat gatgttactg gggatggtca ttttcctgtt
     5041 taccgtgagt tgcgggacgc tctggtatct tggcgggatg atacaggcga acctggaaga
     5101 tatcaggaag caggaagaga cattgcagaa actgaacgcg aagacatggg gcgtggagtt
     5161 cgtacaggac gggaacagga aatttctggt ccttccgtac gggaaatcag cagaggtgat
     5221 tccccatcag gggaaggact gggtacaact gatggagtga cacagagtga cggagctgga
     5281 aagacatttg ctgaacgcat tagagcaact gcaacaggac tatatgcagc ggctgaacga
     5341 atgggagagc gccttcgtgg aattgcagaa aatgttttcg cttacgcaac gggacaacgc
     5401 gatgctgaac gagcgggtca tgaagttgag tcagcaggtg cagcacttga gcgagcggac
     5461 agaacgcttg agccagttat acagcgagaa caggagataa gggacgaacg gctgatacag
     5521 gaacgcgaac atgagttatc
//
