LOCUS       NZ_CP139798             2613 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Escherichia coli strain Nord8 plasmid pNORD8_8.
ACCESSION   NZ_CP139798
VERSION     NZ_CP139798.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38610607
            Assembly: GCF_034421795.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Braun,S.D.
  TITLE     DRESI Project
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Braun,S.D.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2023) Optical Molecular Diagnostics and System
            Technology, Leibniz Institute of Photonic Technology, Albert
            Einstein Strasse 9, Jena 07745, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 23-OCT-2018
            Assembly Method                   :: Flye v. 2.8.1-b1684
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 152.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 04:42:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,503
            CDSs (total)                      :: 5,386
            Genes (coding)                    :: 5,062
            CDSs (with protein)               :: 5,062
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 324
            CDSs (without protein)            :: 324
            Pseudo Genes (ambiguous residues) :: 0 of 324
            Pseudo Genes (frameshifted)       :: 168 of 324
            Pseudo Genes (incomplete)         :: 180 of 324
            Pseudo Genes (internal stop)      :: 45 of 324
            Pseudo Genes (multiple problems)  :: 60 of 324
            Pseudo Genes (short protein)      :: 3 of 324
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139798.1.
            Bacteria and source DNA available from Leibniz-IPHT Jena.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Nord8"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pNORD8_8"
                     /geo_loc_name="Germany"
                     /collection_date="2017-05-12"
     gene            complement(<1..173)
                     /locus_tag="U0546_RS26955"
                     /old_locus_tag="U0546_26955"
                     /pseudo=""
     CDS             complement(<1..173)
                     /locus_tag="U0546_RS26955"
                     /old_locus_tag="U0546_26955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(945..1481)
                     /locus_tag="U0546_RS26960"
                     /old_locus_tag="U0546_26960"
     CDS             complement(945..1481)
                     /locus_tag="U0546_RS26960"
                     /old_locus_tag="U0546_26960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_014342224.1"
                     /translation="MQTQNVVTDKEREIRKRDLEDKDRKNASERRQEQKNQNFTQVYPL
                     GWKRLRELFRKNPGAAELYSMLAENIDGSCGAVVADQTHLASLLGVGRQTISRYVKWLE
                     EQGVLVKIPVAGKVCAYALNPHEVWKGYDNAKPYAAFLTKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(2252..>2613)
                     /locus_tag="U0546_RS26965"
                     /old_locus_tag="U0546_26965"
     CDS             complement(2252..>2613)
                     /locus_tag="U0546_RS26965"
                     /old_locus_tag="U0546_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_322419616.1"
                     /translation="AAELYSMLAENIDGSCGAVVADQTHLASLLGVGRQTISRYVKWLE
                     EQGVLVKIPVAGKVCAYALNPHEVWKGYDNAKPYAAFLTKTLVNKDGDIQRRIMAMFHG
                     NDARQGMEGDTPEAE"
                     /name="helix-turn-helix domain-containing protein"
     Typing          complement(2..173)
                     /name="Col(KPHS6)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="3.38e-85"
     Typing          complement(1305..1481)
                     /name="Col(KPHS6)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
ORIGIN
        1 ggattcttcc tgaaaagctc tctgagacgc ttccagccta aagggtatac ttgggtgaag
       61 ttttggtttt tttgctcctg tcgacgctct gaggcgtttt tacggtcttt atcttcaaga
      121 tctctttttc tgatttcccg ttctttgtcc gtcacaacat tttgagtttg catagactat
      181 ctctcctgcg acaccatgtc gtttgaaagt aacatacagt tgtcattttt ttgtgtcaac
      241 atgtcgcatt tgcacgacat gttgggtata tctgtcgcat cctatagatg cgacaacgtt
      301 gtcgccccta ggatgcgaca acacatttat aaacccataa taaaaacaac aacatacaat
      361 tttttccttc ttatctaaaa agatctcaca gcgagctgcg agatctccag gtagcaccct
      421 cctttcagtc gggcaaaaaa cggtgaaaat ttaaccattg gcaaccgaaa aatcgtgggg
      481 gccccttcag gggttccgat ttttttgttg ccaacgcacc gatcaaaaaa aattggggaa
      541 ggtggttgtg ttcacgcagt gagcgcaacc acctgccaga cgggttttag cgccgcaggc
      601 cgcacatgtt tctgagccaa cggcgaggaa acatataagt gcgcatctct ctaatttcac
      661 gaatttgaga gatgagatca gtctggataa tcggagtact ccgcctctag ccgtcctggt
      721 gaaccagtcc ggcaagaccc acgaaatcac ccgtaaaacg caataaaacg cgttttgacg
      781 cgttttttga cgttctacgg gctcacgtgg gctttgggcg gaaaaatggc ttacgtgcgt
      841 tctgagagcg ttttagggct ttgcagggtg gagtggtgaa gcagctcctt gtggaacctg
      901 taccgccctt gcgggcgggc ttcggcgatt tggcgaacgg gaatctattc ggcttctggc
      961 gtgtcgcctt ccatgccctg acgggcatcg tttccgtgga acatggccat gattcggcgc
     1021 tgaatatcgc cgtctttgtt gactagggtt ttcgtcaaaa atgcggcata cggtttggca
     1081 ttatcgtagc ctttccagac ttcatgcggg ttgagagcat aagcgcatac ctttccagct
     1141 actgggattt ttactaaaac accttgctct tctagccact taacgtacct agatattgtt
     1201 tgtctaccaa caccaagcaa cgatgctaaa tgggtttgat cggcaacaac cgcaccacaa
     1261 gagccatcaa tgttttctgc aagcattgaa taaagctctg ctgcccccgg attcttcctg
     1321 aaaagctctc tgagacgctt ccagcctaaa gggtatactt gggtgaagtt ttggtttttt
     1381 tgctcctgtc gacgctctga ggcgttttta cggtctttat cttcaagatc tctttttctg
     1441 atttcccgtt ctttgtccgt cacaacattt tgagtttgca tagactatct ctcctgcgac
     1501 accatgtcgt ttgaaagtaa catacagttg tcattttttt gtgtcaacat gtcgcatttg
     1561 cacgacatgt tgggtatatc tgtcgcatcc tatagatgcg acaacgttgt cgcccctagg
     1621 atgcgacaac acatttataa acccataata aaaacaacaa catacaattt tttccttctt
     1681 atctaaaaag atctcacagc gagctgcgag atctccaggt agcaccctcc tttcagtcgg
     1741 gcaaaaaacg gtgaaaattt aaccattggc aaccgaaaaa tcgtgggggc cccttcaggg
     1801 gttccgattt ttttgttgcc aacgcaccga tcaaaaaaat tggggaaggt ggttgtgttc
     1861 acgcagtgag cgcaaccacc tgccagacgg gttttagcgc cgcaggccgc acatgtttct
     1921 gagccaacgg cgaggaaaca tataagtgcg catctctcta atttcacgaa tttgagagat
     1981 gagatcagtc tggataatcg gagtactccg cctctagccg tcctggtgaa ccagtccggc
     2041 aagacccacg aaatcacccg taaaacgcaa taaaacgcgt tttgacgcgt tttttgacgt
     2101 tctacgggct cacgtgggct ttgggcggaa aaatggctta cgtgcgttct gagagcgttt
     2161 tagggctttg cagggtggag tggtgaagca gctccttgtg gaacctgtac cgcccttgcg
     2221 ggcgggcttc ggcgatttgg cgaacgggaa tctattcggc ttctggcgtg tcgccttcca
     2281 tgccctgacg ggcatcgttt ccgtggaaca tggccatgat tcggcgctga atatcgccgt
     2341 ctttgttgac tagggttttc gtcaaaaatg cggcatacgg tttggcatta tcgtagcctt
     2401 tccagacttc atgcgggttg agagcataag cgcatacctt tccagctact gggattttta
     2461 ctaaaacacc ttgctcttct agccacttaa cgtacctaga tattgtttgt ctaccaacac
     2521 caagcaacga tgctaaatgg gtttgatcgg caacaaccgc accacaagag ccatcaatgt
     2581 tttctgcaag cattgaataa agctctgctg ccc
//
