LOCUS       NZ_CP139789             8821 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain FF29-002PS2 plasmid
            Col440II, complete sequence.
ACCESSION   NZ_CP139789
VERSION     NZ_CP139789.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38454646
            Assembly: GCF_034421615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8821)
  AUTHORS   Manyahi,J.
  TITLE     Bla NDM5 Klebsiella pneumoniae Tanzania
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8821)
  AUTHORS   Manyahi,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) Clinical Science, University of Bergen,
            Haukelands veien 208, Bergen 5020, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 04:25:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,791
            CDSs (total)                      :: 5,666
            Genes (coding)                    :: 5,526
            CDSs (with protein)               :: 5,526
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 59 of 140
            Pseudo Genes (incomplete)         :: 94 of 140
            Pseudo Genes (internal stop)      :: 14 of 140
            Pseudo Genes (multiple problems)  :: 23 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139789.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8821
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FF29-002PS2"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="Col440II"
                     /geo_loc_name="Tanzania: Dar es Salaam"
                     /lat_lon="6.8165 S 39.2894 E"
                     /collection_date="2022-03-03"
                     /collected_by="Joel Manyahi"
                     /genotype="ST437"
     gene            224..357
                     /locus_tag="U0789_RS28830"
                     /old_locus_tag="U0789_28830"
                     /pseudo=""
     CDS             224..357
                     /locus_tag="U0789_RS28830"
                     /old_locus_tag="U0789_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            612..866
                     /locus_tag="U0789_RS28835"
                     /old_locus_tag="U0789_28835"
     CDS             612..866
                     /locus_tag="U0789_RS28835"
                     /old_locus_tag="U0789_28835"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101361146.1"
                     /translation="MNITINTPSVKTILDVQCDHCNFTGTIDYEAPRISKLTVGGKITF
                     DNALCPQCKTGEIFAPGGQYVRDDATGRMNRTGDANISL"
                     /name="hypothetical protein"
     gene            879..1304
                     /locus_tag="U0789_RS28840"
                     /old_locus_tag="U0789_28840"
     CDS             879..1304
                     /locus_tag="U0789_RS28840"
                     /old_locus_tag="U0789_28840"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046181484.1"
                     /translation="MSFQQVLNEVKVISSNGDTSLAITRYDQQGLNRFLLRDLSHQTET
                     RSNYVNNLEQLCQQAKCNRVILNVNEISESFYMKLITFCNFTLLDAKQDMQHLNGFNYF
                     IVLNSFDENSGHTVWAPSYSPNCVTERDLDEYDPDRW"
                     /name="hypothetical protein"
     gene            complement(1401..1772)
                     /locus_tag="U0789_RS28845"
                     /old_locus_tag="U0789_28845"
     CDS             complement(1401..1772)
                     /locus_tag="U0789_RS28845"
                     /old_locus_tag="U0789_28845"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021314598.1"
                     /translation="MSKKNDLNLSESTLESLREYVDDFGMTVDDVVLKLINENKSLKES
                     LGQDFVLSGEKEAHHFKDYVIHKLPKGKILVTHNGLKASNMKKILIEAGRELGMDENEL
                     NPDFTTYEIGRFLFKHLHA"
                     /name="hypothetical protein"
     gene            complement(2277..2492)
                     /locus_tag="U0789_RS28850"
                     /old_locus_tag="U0789_28850"
     CDS             complement(2277..2492)
                     /locus_tag="U0789_RS28850"
                     /old_locus_tag="U0789_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2499..2996)
                     /locus_tag="U0789_RS28855"
                     /old_locus_tag="U0789_28855"
     CDS             complement(2499..2996)
                     /locus_tag="U0789_RS28855"
                     /old_locus_tag="U0789_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3037..3735)
                     /locus_tag="U0789_RS28860"
                     /old_locus_tag="U0789_28860"
                     /pseudo=""
     CDS             complement(<3037..3735)
                     /locus_tag="U0789_RS28860"
                     /old_locus_tag="U0789_28860"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3674..4015)
                     /locus_tag="U0789_RS28865"
                     /old_locus_tag="U0789_28865"
     CDS             complement(3674..4015)
                     /locus_tag="U0789_RS28865"
                     /old_locus_tag="U0789_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4635..4768
                     /locus_tag="U0789_RS28870"
                     /old_locus_tag="U0789_28870"
                     /pseudo=""
     CDS             4635..4768
                     /locus_tag="U0789_RS28870"
                     /old_locus_tag="U0789_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            5023..5277
                     /locus_tag="U0789_RS28875"
                     /old_locus_tag="U0789_28875"
     CDS             5023..5277
                     /locus_tag="U0789_RS28875"
                     /old_locus_tag="U0789_28875"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_101361146.1"
                     /translation="MNITINTPSVKTILDVQCDHCNFTGTIDYEAPRISKLTVGGKITF
                     DNALCPQCKTGEIFAPGGQYVRDDATGRMNRTGDANISL"
                     /name="hypothetical protein"
     gene            5290..5715
                     /locus_tag="U0789_RS28880"
                     /old_locus_tag="U0789_28880"
     CDS             5290..5715
                     /locus_tag="U0789_RS28880"
                     /old_locus_tag="U0789_28880"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_046181484.1"
                     /translation="MSFQQVLNEVKVISSNGDTSLAITRYDQQGLNRFLLRDLSHQTET
                     RSNYVNNLEQLCQQAKCNRVILNVNEISESFYMKLITFCNFTLLDAKQDMQHLNGFNYF
                     IVLNSFDENSGHTVWAPSYSPNCVTERDLDEYDPDRW"
                     /name="hypothetical protein"
     gene            complement(5812..6183)
                     /locus_tag="U0789_RS28885"
                     /old_locus_tag="U0789_28885"
     CDS             complement(5812..6183)
                     /locus_tag="U0789_RS28885"
                     /old_locus_tag="U0789_28885"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021314598.1"
                     /translation="MSKKNDLNLSESTLESLREYVDDFGMTVDDVVLKLINENKSLKES
                     LGQDFVLSGEKEAHHFKDYVIHKLPKGKILVTHNGLKASNMKKILIEAGRELGMDENEL
                     NPDFTTYEIGRFLFKHLHA"
                     /name="hypothetical protein"
     gene            complement(6688..6903)
                     /locus_tag="U0789_RS28890"
                     /old_locus_tag="U0789_28890"
     CDS             complement(6688..6903)
                     /locus_tag="U0789_RS28890"
                     /old_locus_tag="U0789_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_015328239.1"
                     /translation="MTELEKQLLSALEQLQQDYSRRLDEWESAFEEWRKMSGLMQRENA
                     ALSERVSGLSRQVQSLSEQLRRLSTG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(6910..7407)
                     /locus_tag="U0789_RS28895"
                     /old_locus_tag="U0789_28895"
     CDS             complement(6910..7407)
                     /locus_tag="U0789_RS28895"
                     /old_locus_tag="U0789_28895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_020325392.1"
                     /translation="MSSLLTASRDFEEKSKAQQQLTEERLKAAFSKHENVVKSELNASA
                     KRINDAISAHEQGMNAAMQSNRLSVLRMVGRTWLTITMVSGLLFASLSGVLWYQGNRIA
                     ANLAEIRQQRDTLSKLQMQTWGVTYLENRNGRFLVLPEGMKAETGRTVDNKTRNAVKLV
                     RE"
                     /name="MbeB family mobilization protein"
     gene            complement(<7448..8146)
                     /locus_tag="U0789_RS28900"
                     /old_locus_tag="U0789_28900"
                     /pseudo=""
     CDS             complement(<7448..8146)
                     /locus_tag="U0789_RS28900"
                     /old_locus_tag="U0789_28900"
                     /inference="COORDINATES: protein motif:HMM:NF015397.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(8085..8426)
                     /locus_tag="U0789_RS28905"
                     /old_locus_tag="U0789_28905"
     CDS             complement(8085..8426)
                     /locus_tag="U0789_RS28905"
                     /old_locus_tag="U0789_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_071599364.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPTR
                     AGRLPSISPALLRQLAGMGNNLNQIARKVNAGGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGPDDDC"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(4110..4285)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="82.32"
                     /coverage="100.0"
                     /evalue="5.910000000000001e-33"
     Typing          complement(8521..8696)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="82.32"
                     /coverage="100.0"
                     /evalue="5.910000000000001e-33"
     Typing          2..325
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.801"
                     /coverage="83.0"
                     /evalue="1.81e-141"
     Typing          4231..4744
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.103"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          8642..8821
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.868"
                     /coverage="99.0"
                     /evalue="1.54e-33"
     Typing          complement(2162..3684)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3681..3994)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.3500000000000001e-58"
     Typing          complement(6573..8095)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.606"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8092..8405)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.3500000000000001e-58"
ORIGIN
        1 ccgccccctg acgagcatca cagaatctga tgctcaaatc agtggtggcg aaacccgaca
       61 ggactataaa gatcccaggc gtttccccct ggtagctccc tcgtgcgctc tcctgttcct
      121 gcctttcggt ttaccggtgt cattccgctg ttatggccgc gtttttctca ttccacgcct
      181 gacactcagt tccgggtagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
      241 tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggaaagaca
      301 tacaaagcgc caatggcaac ggccacaggt aacggattta gaggagttga gtcttgaagt
      361 tatgcgccgg ttaaggctaa actgaaagga cagatttggt gactgcgctc ctccaagcca
      421 gttacctcgg ttcaaagagt tgctgctcca agaaccttcg aaaaaaccgc ccagcaaggc
      481 ggttttttcg tttacaggaa tgaagactac gacgagagga acacgatctc aagaagatcc
      541 ttcgttttca ttagctaagc attctctgat aaagtcatca caaccacttg taatgataag
      601 gattgttgag aatgaatata actatcaata ccccgtcagt aaaaaccatc cttgatgtac
      661 agtgtgatca ctgtaacttc actggcacta ttgattatga agcccctcga atttcaaagc
      721 ttactgtcgg cggaaaaata accttcgaca atgctttatg cccgcaatgt aaaacaggtg
      781 aaattttcgc tccaggtggt caatacgtta gagatgatgc tactggaaga atgaatcgca
      841 caggcgatgc gaacattagt ctataaggaa cttatcatat gagttttcag caagttttga
      901 atgaggtaaa ggttatttca agcaatggtg atacaagcct agcgattacc agatatgacc
      961 aacaaggatt gaaccgtttt cttttacgtg acttatctca tcagaccgag actcgtagta
     1021 actatgtaaa taatcttgag caattatgcc agcaggcaaa atgtaacagg gttattttga
     1081 acgtgaatga aatttcagag tcgttttata tgaaattaat aactttttgt aatttcactc
     1141 tgttagacgc caaacaagat atgcaacatt tgaatggctt caattacttt attgttctta
     1201 attcttttga tgaaaacagt gggcatacag tttgggcacc atcgtatagc cccaattgtg
     1261 taactgagcg cgatttagat gaatacgacc cagatcgttg gtaactcaga tcgcctaata
     1321 aaaaatccgt atagcaccta aatttaccat aacatccggg cagctaagtt cgattcctgc
     1381 ccttcaggcg tgcaggattg ttacgcatgt aggtgcttaa acaaaaaacg ccctatctca
     1441 taggttgtga aatctggatt gagttcattt tcatccatgc ctagctccct ccccgcctca
     1501 atcaatatct tcttcatgtt gctggcctta agcccattat gggtaaccag aatttttcct
     1561 ttgggcagtt tatgaatcac ataatcttta aagtggtgcg cttccttttc gccagataac
     1621 acaaagtctt gtccaagtga ttccttcaat gacttattct cattaattaa cttcagaaca
     1681 acatcatcaa cagtcatgcc gaagtcatcg acatactccc tgaggctttc taacgttgat
     1741 tcactgaggt ttaaatcgtt ttttttactc atgtagtcac cactatgcta gttgtcagaa
     1801 aagaaaaaaa caacacaagt agtatagtac acatattttt agtattgaac aagcaacata
     1861 gaagtcactg atcgctggtt caaaaagaac agctacaccg ctggatttac cataacatct
     1921 cttacccgca ccgcgccgga ggcactcagg gcgtttctga tggctgacgg gcaaaaggtg
     1981 gtggcaagta caccgaaaac caccagaaac gcacctgatt tattttacgc atatgcatca
     2041 cttattcaca tcactgcata gccggtgatt tcggttaaaa aagactctct gtgtgctgaa
     2101 atcgcagaaa atggccatga tccccgtcac gctcaggcca cctgagacgg gcgtttatca
     2161 gtctccatac tccctgtcac catgagataa tgtgcgttgc cgttccggta ccagatctgc
     2221 tgtcttctct ggttcctttt ccgttctttg cccgtatcgc tgttgctcaa tctcattcag
     2281 cctgtcgata accggcgcag ctgctcgctc aaactctgca cctgcctgct caagcccgac
     2341 acgcgctcgc tcagcgccgc gttctcccgt tgcataagac cagacatctt ccgccattcc
     2401 tcgaaggcgc tctcccactc gtccagcctt ctcgagtagt cctgctgtag ctgctctaac
     2461 gcgctcagca attgtttttc cagttccgtc attctgctct actcccgtac cagcttcacc
     2521 gcgttccgcg tcttgttgtc caccgtccgg cccgtttcgg ctttcatccc ctccggcagc
     2581 accagaaacc gtccgttgcg gttctccaga taggtcacgc cccacgtctg catctgcagt
     2641 ttcgacagcg tatctctctg ctgcctgatt tccgccaggt tcgccgctat ccggttcccc
     2701 tggtaccaca gcaccccgct caggctggcg aacagcagcc ctgacaccat ggtgatggtc
     2761 agccacgtcc gcccgaccat tcgcagcacg ctcagccggt tcgactgcat cgccgcgttc
     2821 atcccctgtt cgtgggcgct gatggcgtcg ttgattctct tcgcgcttgc gttcagttcc
     2881 gatttgacaa cgttctcgtg cttgctgaat gcggctttca gcctctcttc ggtaagctgc
     2941 tgctgtgctt tcgatttctc ctcgaagtcc ctcgaggccg ttaaaagact gctcataaat
     3001 ggctcccctc agtcggatat ttcgcccccc gtccgggtcg gcaatgctga tactgttttt
     3061 tgtggttctg accacctcaa aaccggccgc tgtgagcgcc cctgtgacgt catcacgcgt
     3121 tttcagctct cctgatgagg ctaacatcag caaaccccgt gtaatcgtct ctgcggcttc
     3181 ctgcttcgtt tctggcagtc ctgacggcgt gaccagcgcc cgccggttct ccgggtcgtt
     3241 cggatcatgt agccccagcc tgccgttgac cactgtctgc cacgcatcga tgcgcggccg
     3301 gtcggcacga tcgtaatacg gctggaggcg tttccccgtc agtaattcgg tattggggat
     3361 cagaaaattc agctcaagac gacccttgtc tgcgtgttcc acccacagaa tgctgtactg
     3421 atctttatcg agtccgggca tcagaacccg ctcaaagctg gccatcagtt tttcacgctg
     3481 acccggcggc aggtccgcct ccgcgaatga cagcaccccc gaggtatatt ttttcacata
     3541 cggcgacgca tcaatcagct cccggacttc ttccggcttc ccctgcagga cgctggcacc
     3601 ttcacgctgc cggtctttac ccagaagata atccacagga ccggcgcccc cgccgcgacc
     3661 ccggggatgg aatttaacaa tcatcgtccg gccctttttc cagtacggca tgccgcaacc
     3721 gttccagacc ggcatcgatc gccatcagcg ctgccacgat ctgcacccgg tcatgaccgg
     3781 caccaccggc gttcactttc cgggcgatct ggttcaggtt attgcccatg ccagcaagct
     3841 gacgcagcag cgcgggcgaa atcgagggaa gcctgccggc gcgtgtcggt ttctcatcaa
     3901 ggcaggtctg gcgcatccac gccgccagtt gttttccgtc gcaccgctca agcaaccgcc
     3961 ggtgttcgtc ttccgtcacc cacatcgtga gcatcttgtt gcgtttatct gccagaatcg
     4021 cctcctgata acgccgtgac ggacgggaaa gcctttcccg gtcggcgcgg ggttggacaa
     4081 gccgcaggcg cgtcagtggc ttttagcggg tttcggggcg cagccctgaa ccagtcacgt
     4141 agcgctagcg gagtgtatac tggcttacta tgttggcatt ggtatgggta tcagcgaaag
     4201 tgctatatat ggcaggtatg acatgcagct gccgggcgta agacagataa tggttgtgca
     4261 ggagatatga cgcctcctcg ctcactcggt cgctgacgct cctgccgttc ggctgcggcg
     4321 ggcgtaaccg gcacgcggag aaatgcagag acatatgccg tgaaggcaag gaagagaaaa
     4381 ggacgcggcg ctgccgtttt tccataggct ccgcccccct gacgagcatc acagaatctg
     4441 atgctcaaat cagtggtggc gaaacccgac aggactataa agatcccagg cgtttccccc
     4501 tggtagctcc ctcgtgcgct ctcctgttcc tgcctttcgg tttaccggtg tcattccgct
     4561 gttatggccg cgtttttctc attccacgcc tgacactcag ttccgggtag gcagttcgct
     4621 ccaagctgga ctgtatgcac gaaccccccg ttcagtccga ccgctgcgcc ttatccggta
     4681 actatcgtct tgagtccaac ccggaaagac atacaaagcg ccaatggcaa cggccacagg
     4741 taacggattt agaggagttg agtcttgaag ttatgcgccg gttaaggcta aactgaaagg
     4801 acagatttgg tgactgcgct cctccaagcc agttacctcg gttcaaagag ttgctgctcc
     4861 aagaaccttc gaaaaaaccg cccagcaagg cggttttttc gtttacagga atgaagacta
     4921 cgacgagagg aacacgatct caagaagatc cttcgttttc attagctaag cattctctga
     4981 taaagtcatc acaaccactt gtaatgataa ggattgttga gaatgaatat aactatcaat
     5041 accccgtcag taaaaaccat ccttgatgta cagtgtgatc actgtaactt cactggcact
     5101 attgattatg aagcccctcg aatttcaaag cttactgtcg gcggaaaaat aaccttcgac
     5161 aatgctttat gcccgcaatg taaaacaggt gaaattttcg ctccaggtgg tcaatacgtt
     5221 agagatgatg ctactggaag aatgaatcgc acaggcgatg cgaacattag tctataagga
     5281 acttatcata tgagttttca gcaagttttg aatgaggtaa aggttatttc aagcaatggt
     5341 gatacaagcc tagcgattac cagatatgac caacaaggat tgaaccgttt tcttttacgt
     5401 gacttatctc atcagaccga gactcgtagt aactatgtaa ataatcttga gcaattatgc
     5461 cagcaggcaa aatgtaacag ggttattttg aacgtgaatg aaatttcaga gtcgttttat
     5521 atgaaattaa taactttttg taatttcact ctgttagacg ccaaacaaga tatgcaacat
     5581 ttgaatggct tcaattactt tattgttctt aattcttttg atgaaaacag tgggcataca
     5641 gtttgggcac catcgtatag ccccaattgt gtaactgagc gcgatttaga tgaatacgac
     5701 ccagatcgtt ggtaactcag atcgcctaat aaaaaatccg tatagcacct aaatttacca
     5761 taacatccgg gcagctaagt tcgattcctg cccttcaggc gtgcaggatt gttacgcatg
     5821 taggtgctta aacaaaaaac gccctatctc ataggttgtg aaatctggat tgagttcatt
     5881 ttcatccatg cctagctccc tccccgcctc aatcaatatc ttcttcatgt tgctggcctt
     5941 aagcccatta tgggtaacca gaatttttcc tttgggcagt ttatgaatca cataatcttt
     6001 aaagtggtgc gcttcctttt cgccagataa cacaaagtct tgtccaagtg attccttcaa
     6061 tgacttattc tcattaatta acttcagaac aacatcatca acagtcatgc cgaagtcatc
     6121 gacatactcc ctgaggcttt ctaacgttga ttcactgagg tttaaatcgt tttttttact
     6181 catgtagtca ccactatgct agttgtcaga aaagaaaaaa acaacacaag tagtatagta
     6241 cacatatttt tagtattgaa caagcaacat agaagtcact gatcgctggt tcaaaaagaa
     6301 cagctacacc gctggattta ccataacatc tcttacccgc accgcgccgg aggcactcag
     6361 ggcgtttctg atggctgacg ggcaaaaggt ggtggcaagt acaccgaaaa ccaccagaaa
     6421 cgcacctgat ttattttacg catatgcatc acttattcac atcactgcat agccggtgat
     6481 ttcggttaaa aaagactctc tgtgtgctga aatcgcagaa aatggccatg atccccgtca
     6541 cgctcaggcc acctgagacg ggcgtttatc agtctccata ctccctgtca ccatgagata
     6601 atgtgcgttg ccgttccggt accagatctg ctgtcttctc tggttccttt tccgttcttt
     6661 gcccgtatcg ctgttgctca atctcattca gcctgtcgat aaccggcgca gctgctcgct
     6721 caaactctgc acctgcctgc tcaagcccga cacgcgctcg ctcagcgccg cgttctcccg
     6781 ttgcataaga ccagacatct tccgccattc ctcgaaggcg ctctcccact cgtccagcct
     6841 tctcgagtag tcctgctgta gctgctctaa cgcgctcagc aattgttttt ccagttccgt
     6901 cattctgctc tactcccgta ccagcttcac cgcgttccgc gtcttgttgt ccaccgtccg
     6961 gcccgtttcg gctttcatcc cctccggcag caccagaaac cgtccgttgc ggttctccag
     7021 ataggtcacg ccccacgtct gcatctgcag tttcgacagc gtatctctct gctgcctgat
     7081 ttccgccagg ttcgccgcta tccggttccc ctggtaccac agcaccccgc tcaggctggc
     7141 gaacagcagc cctgacacca tggtgatggt cagccacgtc cgcccgacca ttcgcagcac
     7201 gctcagccgg ttcgactgca tcgccgcgtt catcccctgt tcgtgggcgc tgatggcgtc
     7261 gttgattctc ttcgcgcttg cgttcagttc cgatttgaca acgttctcgt gcttgctgaa
     7321 tgcggctttc agcctctctt cggtaagctg ctgctgtgct ttcgatttct cctcgaagtc
     7381 cctcgaggcc gttaaaagac tgctcataaa tggctcccct cagtcggata tttcgccccc
     7441 cgtccgggtc ggcaatgctg atactgtttt ttgtggttct gaccacctca aaaccggccg
     7501 ctgtgagcgc ccctgtgacg tcatcacgcg ttttcagctc tcctgatgag gctaacatca
     7561 gcaaaccccg tgtaatcgtc tctgcggctt cctgcttcgt ttctggcagt cctgacggcg
     7621 tgaccagcgc ccgccggttc tccgggtcgt tcggatcatg tagccccagc ctgccgttga
     7681 ccactgtctg ccacgcatcg atgcgcggcc ggtcggcacg atcgtaatac ggctggaggc
     7741 gtttccccgt cagtaattcg gtattgggga tcagaaaatt cagctcaaga cgacccttgt
     7801 ctgcgtgttc cacccacaga atgctgtact gatctttatc gagtccgggc atcagaaccc
     7861 gctcaaagct ggccatcagt ttttcacgct gacccggcgg caggtccgcc tccgcgaatg
     7921 acagcacccc cgaggtatat tttttcacat acggcgacgc atcaatcagc tcccggactt
     7981 cttccggctt cccctgcagg acgctggcac cttcacgctg ccggtcttta cccagaagat
     8041 aatccacagg accggcgccc ccgccgcgac cccggggatg gaatttaaca atcatcgtcc
     8101 ggcccttttt ccagtacggc atgccgcaac cgttccagac cggcatcgat cgccatcagc
     8161 gctgccacga tctgcacccg gtcatgaccg gcaccaccgg cgttcacttt ccgggcgatc
     8221 tggttcaggt tattgcccat gccagcaagc tgacgcagca gcgcgggcga aatcgaggga
     8281 agcctgccgg cgcgtgtcgg tttctcatca aggcaggtct ggcgcatcca cgccgccagt
     8341 tgttttccgt cgcaccgctc aagcaaccgc cggtgttcgt cttccgtcac ccacatcgtg
     8401 agcatcttgt tgcgtttatc tgccagaatc gcctcctgat aacgccgtga cggacgggaa
     8461 agcctttccc ggtcggcgcg gggttggaca agccgcaggc gcgtcagtgg cttttagcgg
     8521 gtttcggggc gcagccctga accagtcacg tagcgctagc ggagtgtata ctggcttact
     8581 atgttggcat tggtatgggt atcagcgaaa gtgctatata tggcaggtat gacatgcagc
     8641 tgccgggcgt aagacagata atggttgtgc aggagatatg acgcctcctc gctcactcgg
     8701 tcgctgacgc tcctgccgtt cggctgcggc gggcgtaacc ggcacgcgga gaaatgcaga
     8761 gacatatgcc gtgaaggcaa ggaagagaaa aggacgcggc gctgccgttt ttccataggc
     8821 t
//
