LOCUS       NZ_CP139788            10677 bp    DNA     linear   CON 21-DEC-2023
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain FF29-002PS2 plasmid
            Col440I, complete sequence.
ACCESSION   NZ_CP139788
VERSION     NZ_CP139788.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38454646
            Assembly: GCF_034421615.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Manyahi,J.
  TITLE     Bla NDM5 Klebsiella pneumoniae Tanzania
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10677)
  AUTHORS   Manyahi,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) Clinical Science, University of Bergen,
            Haukelands veien 208, Bergen 5020, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. May-2022
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/21/2023 04:25:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,791
            CDSs (total)                      :: 5,666
            Genes (coding)                    :: 5,526
            CDSs (with protein)               :: 5,526
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 140
            CDSs (without protein)            :: 140
            Pseudo Genes (ambiguous residues) :: 0 of 140
            Pseudo Genes (frameshifted)       :: 59 of 140
            Pseudo Genes (incomplete)         :: 94 of 140
            Pseudo Genes (internal stop)      :: 14 of 140
            Pseudo Genes (multiple problems)  :: 23 of 140
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139788.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="FF29-002PS2"
                     /isolation_source="Pus"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="Col440I"
                     /geo_loc_name="Tanzania: Dar es Salaam"
                     /lat_lon="6.8165 S 39.2894 E"
                     /collection_date="2022-03-03"
                     /collected_by="Joel Manyahi"
                     /genotype="ST437"
     gene            144..620
                     /locus_tag="U0789_RS28740"
                     /old_locus_tag="U0789_28740"
     CDS             144..620
                     /locus_tag="U0789_RS28740"
                     /old_locus_tag="U0789_28740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(827..940)
                     /locus_tag="U0789_RS28745"
                     /old_locus_tag="U0789_28745"
     CDS             complement(827..940)
                     /locus_tag="U0789_RS28745"
                     /old_locus_tag="U0789_28745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            1356..1547
                     /locus_tag="U0789_RS28750"
                     /old_locus_tag="U0789_28750"
     CDS             1356..1547
                     /locus_tag="U0789_RS28750"
                     /old_locus_tag="U0789_28750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1547..1969
                     /locus_tag="U0789_RS28755"
                     /old_locus_tag="U0789_28755"
     CDS             1547..1969
                     /locus_tag="U0789_RS28755"
                     /old_locus_tag="U0789_28755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2833..3057
                     /locus_tag="U0789_RS28760"
                     /old_locus_tag="U0789_28760"
     CDS             2833..3057
                     /locus_tag="U0789_RS28760"
                     /old_locus_tag="U0789_28760"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            3059..3703
                     /locus_tag="U0789_RS28765"
                     /old_locus_tag="U0789_28765"
     CDS             3059..3703
                     /locus_tag="U0789_RS28765"
                     /old_locus_tag="U0789_28765"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            3703..4179
                     /locus_tag="U0789_RS28770"
                     /old_locus_tag="U0789_28770"
     CDS             3703..4179
                     /locus_tag="U0789_RS28770"
                     /old_locus_tag="U0789_28770"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(4386..4499)
                     /locus_tag="U0789_RS28775"
                     /old_locus_tag="U0789_28775"
     CDS             complement(4386..4499)
                     /locus_tag="U0789_RS28775"
                     /old_locus_tag="U0789_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            4915..5106
                     /locus_tag="U0789_RS28780"
                     /old_locus_tag="U0789_28780"
     CDS             4915..5106
                     /locus_tag="U0789_RS28780"
                     /old_locus_tag="U0789_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5106..5528
                     /locus_tag="U0789_RS28785"
                     /old_locus_tag="U0789_28785"
     CDS             5106..5528
                     /locus_tag="U0789_RS28785"
                     /old_locus_tag="U0789_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            6392..6616
                     /locus_tag="U0789_RS28790"
                     /old_locus_tag="U0789_28790"
     CDS             6392..6616
                     /locus_tag="U0789_RS28790"
                     /old_locus_tag="U0789_28790"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            6618..7262
                     /locus_tag="U0789_RS28795"
                     /old_locus_tag="U0789_28795"
     CDS             6618..7262
                     /locus_tag="U0789_RS28795"
                     /old_locus_tag="U0789_28795"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_044816498.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNLIVKTNNEIIFSFVRKVSGVVKVTGRAYIGDSLDNSIAIKSLFKMMEW"
                     /name="hypothetical protein"
     gene            7262..7738
                     /locus_tag="U0789_RS28800"
                     /old_locus_tag="U0789_28800"
     CDS             7262..7738
                     /locus_tag="U0789_RS28800"
                     /old_locus_tag="U0789_28800"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_077861907.1"
                     /translation="MTVLSTLNNLKSIPCINAFTGRANNSYLWRYVMEDHKDEFGATTI
                     KCFYLREEISEDYVSIYHSNKDYYEGMVRDVHKRIPVGKTKPAIYLSFDAAEVIEQVNK
                     PDKCIDIKDEGYPHLGLWYLFDINDEFTLIEVTTTILELSSFFQRNEGGVVVPL"
                     /name="hypothetical protein"
     gene            complement(7945..8058)
                     /locus_tag="U0789_RS28805"
                     /old_locus_tag="U0789_28805"
     CDS             complement(7945..8058)
                     /locus_tag="U0789_RS28805"
                     /old_locus_tag="U0789_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071653267.1"
                     /translation="MHEPPVQSDCCAFSGNYQLESNPERHDEAPLAAATGN"
                     /name="replication initiation protein"
     gene            8474..8665
                     /locus_tag="U0789_RS28810"
                     /old_locus_tag="U0789_28810"
     CDS             8474..8665
                     /locus_tag="U0789_RS28810"
                     /old_locus_tag="U0789_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_044816495.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSGLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            8665..9087
                     /locus_tag="U0789_RS28815"
                     /old_locus_tag="U0789_28815"
     CDS             8665..9087
                     /locus_tag="U0789_RS28815"
                     /old_locus_tag="U0789_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_044816494.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKNTKVVD
                     TSELIRVFGSLYGGGDTVKHDVKGDAGATGVNISGLHDLQHKIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKLEPSPQVHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            9951..10175
                     /locus_tag="U0789_RS28820"
                     /old_locus_tag="U0789_28820"
     CDS             9951..10175
                     /locus_tag="U0789_RS28820"
                     /old_locus_tag="U0789_28820"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071653266.1"
                     /translation="MSNKDSLERKVTPRKNEPVFPMALRVGIAEDICFLELVDFPEKES
                     GRVFYSIALTKKHAKNLIDGLNKFVESGD"
                     /name="hypothetical protein"
     gene            10177..>10677
                     /locus_tag="U0789_RS28825"
                     /old_locus_tag="U0789_28825"
     CDS             10177..>10677
                     /locus_tag="U0789_RS28825"
                     /old_locus_tag="U0789_28825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_322426109.1"
                     /translation="MKYINHCDSEDYYITNDSIDLGVRYDVSDYNKLELNDLRDPDLFK
                     VLGRAFVVVENSVWESWNDDVKTLDCSGDGYESILKHYESLELSGEFLLFFKKIRSAIS
                     VYNREYEFCTGDLEPSLQAIVQLIRYAPELAAKKDAQIYFDKKSECFGLIYKQTKKSKG
                     TLNL"
                     /name="hypothetical protein"
     Typing          complement(828..1235)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
     Typing          complement(4387..4794)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
     Typing          complement(7946..8353)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.39"
                     /coverage="99.0"
                     /evalue="1.26e-177"
ORIGIN
        1 atagtgaaaa caaataacga gattattttt tcctttgtta gaaaagtttc tggagtggtt
       61 aaggttacag gtagggcata tattggcgat agccttgata actccattgc tatcaaatca
      121 ttatttaaaa tgatggagtg gtgatgactg tgttatcaac tttaaataac ctcaaatcaa
      181 tcccttgtat taatgcattt acgggcagag caaataattc atatttatgg cgttatgtta
      241 tggaagatca taaagatgag tttggcgcta caactattaa gtgtttttat cttagggagg
      301 aaatttcaga ggattatgtg tctatttatc attcaaataa agattattat gaaggaatgg
      361 taagggatgt acataaaaga atccctgtag ggaaaaccaa acctgccata tatctctcat
      421 ttgatgcagc ggaagtgatc gagcaagtta acaaaccaga taaatgtatt gatataaagg
      481 acgaaggtta ccctcacttg ggtttgtggt atttatttga tattaatgat gaatttactt
      541 tgattgaggt gactacaact attttggaat tatcctcttt ttttcaaaga aatgaaggtg
      601 gcgtggttgt tcctctttag cgaaaaccgt cttgacgtca tcaagtgtga cctagtactc
      661 ttcttgaaaa agaaaaaccg cctttgcggg gcggtttttc ttaggttcta agagtttgcg
      721 gctctatgaa ccaaggtaac tggctagtta gaggagcgca gtaaccaaaa tcgttctttc
      781 agtttagcct taactggcac ataacttcaa gactaactcc tctaaatcag ttaccagtgg
      841 ctgctgccag tggcgcttcg tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg
      901 aaaaggcgca gcagtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa
      961 ctgcctaccc ggaactgagt gtcaggcgtg gaatgagata aacgcggcca taacagcgga
     1021 atgacaccgg taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc accaggggga
     1081 aacgcctggt atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt
     1141 tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gccgctgtgc ggcctctcct
     1201 tctccccatc acgcgatcgc tcggttttct cgagctttta tacgcatgct cctgccgctc
     1261 gccgcagccg aacggcagga gcgtagcgac tgagtgagcg aggaagcgga aaagagaaca
     1321 tccctacact gagcactgac gctctgaggc ctcttatgac gaaacaacag aaaacagcgc
     1381 tgaacatggc taaattcatc cagaatcagt ctctgctcct gctggagaag cttaacgagc
     1441 ttgatcttga tgtagaggcc gacctttgtg agaaactcca cgatgatgcg gagcatcttt
     1501 tccgtacact gtcttcggga ctggatgaat tacaggatgg aaactgatgg ccagagtgtc
     1561 tattagtgaa gcggctcggc ttgttaaggt tagtcgcccc acaatttaca agatgattaa
     1621 cagtgggaaa ctaagttaca cctctgttgt aaagcatggg aaaaatacta aagttgttga
     1681 tacatcagag cttattaggg tttttggctc gctttatggt gggggcgaca ctgtaaaaca
     1741 tgatgtaaaa ggtgacgcag gcgctacagg ggttaacatc tctggtttac atgatttaca
     1801 gcataaaatt gctttgcttg aagctgaaaa cgatggtctt aaaggggctg ttaaagctcg
     1861 tgatgaacat atcgattctc ttcgccaggc tatgcagctt ctggaacaca agctcgaacc
     1921 ctctccacag gttcattctc cgtggtggaa attctggaag aaatcctgac cccgtcatgc
     1981 ttcagttttc ctctccggcc attccgggga gttcctgaac cttcccaaaa atcgccagat
     2041 cgctgaacgc tcctcaccac taaaaatatc cacaccccac gcaaaattaa aattttgcag
     2101 atttgtattt tatatcagta gtttacgttc tataaatttc gtttttttaa ttttatttat
     2161 acagatccga ctctatgacg actcgtcata gagtccccgg cgagtcgtca tagagtcggt
     2221 aacgactcag gactataaaa acaataagtt aacaacgacc cgactctaga cccgactccc
     2281 cgtttggttg ttttttagac gtgacgactc tgggtacgac tctagtgaag agtcgccggt
     2341 gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg attgccggcc gtcgacgaac
     2401 ccgattcctg aacgtgctga aaaccggttt tccggctgcg ccggcaacgc ttcacggcca
     2461 cgcaataaat tgcgtccccg ttcaccgttt tcggatggga agaacgccgg aaacaggact
     2521 taccctggct gaatagcgtg aattttcgcg ctatgcactt gcgtgcatac tcatgcatgc
     2581 cgtaaaaaca gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctattttac
     2641 cggtttcccg tcagaaacgc cctgaggccg ttttagcggt gcgtacaatt ttggtgttat
     2701 gttaaatcat ggccacaaaa ttccataaag tgaccaatgt aatgttaaat gagcattgtg
     2761 gatgaaagat gttgttttat ggtattattt tttatgttct aaacttaaat ttatttctat
     2821 cggggtatta taatgagcaa taaagattct ttagaaagaa aagtaacacc aagaaaaaat
     2881 gaacctgtat tcccgatggc tctaagagtt gggatagctg aagatatttg ttttttagag
     2941 ttagtggact ttcctgaaaa agaatcagga agggtttttt attccatagc tctaaccaaa
     3001 aaacatgcaa agaaccttat cgatggattg aataaattcg ttgagtcagg tgattagtat
     3061 gaaatacatt aaccactgcg atagtgaaga ttattatatt actaatgata gcattgatct
     3121 cggtgttcgt tatgatgttt ctgattataa taagttagag ctaaatgatt taagagatcc
     3181 ggatttgttt aaagttttgg gtcgagcatt tgttgttgtt gagaattctg tttgggagtc
     3241 ttggaatgat gatgttaaaa cattagattg ctctggtgac ggttatgagt caattttaaa
     3301 acattacgaa tcattagaat tatcaggtga attcttattg ttttttaaaa aaataagaag
     3361 tgccatttct gtttataata gagaatacga gttctgcact ggtgacctag aaccatcttt
     3421 gcaggctatt gttcagttga ttaggtatgc ccctgaactt gctgcaaaaa aagacgctca
     3481 gatttacttt gataaaaaat cagagtgttt tgggcttata tataaacaaa ctaagaaaag
     3541 caaaggcacg ctaaatttaa tagtgaaaac aaataacgag attatttttt cctttgttag
     3601 aaaagtttct ggagtggtta aggttacagg tagggcatat attggcgata gccttgataa
     3661 ctccattgct atcaaatcat tatttaaaat gatggagtgg tgatgactgt gttatcaact
     3721 ttaaataacc tcaaatcaat cccttgtatt aatgcattta cgggcagagc aaataattca
     3781 tatttatggc gttatgttat ggaagatcat aaagatgagt ttggcgctac aactattaag
     3841 tgtttttatc ttagggagga aatttcagag gattatgtgt ctatttatca ttcaaataaa
     3901 gattattatg aaggaatggt aagggatgta cataaaagaa tccctgtagg gaaaaccaaa
     3961 cctgccatat atctctcatt tgatgcagcg gaagtgatcg agcaagttaa caaaccagat
     4021 aaatgtattg atataaagga cgaaggttac cctcacttgg gtttgtggta tttatttgat
     4081 attaatgatg aatttacttt gattgaggtg actacaacta ttttggaatt atcctctttt
     4141 tttcaaagaa atgaaggtgg cgtggttgtt cctctttagc gaaaaccgtc ttgacgtcat
     4201 caagtgtgac ctagtactct tcttgaaaaa gaaaaaccgc ctttgcgggg cggtttttct
     4261 taggttctaa gagtttgcgg ctctatgaac caaggtaact ggctagttag aggagcgcag
     4321 taaccaaaat cgttctttca gtttagcctt aactggcaca taacttcaag actaactcct
     4381 ctaaatcagt taccagtggc tgctgccagt ggcgcttcgt cgtgtctttc cgggttggac
     4441 tcaagttgat agttaccgga aaaggcgcag cagtcggact gaacgggggg ttcgtgcata
     4501 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagataa
     4561 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     4621 gagggagcca ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     4681 ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat ggaaaaacgg
     4741 ccgctgtgcg gcctctcctt ctccccatca cgcgatcgct cggttttctc gagcttttat
     4801 acgcatgctc ctgccgctcg ccgcagccga acggcaggag cgtagcgact gagtgagcga
     4861 ggaagcggaa aagagaacat ccctacactg agcactgacg ctctgaggcc tcttatgacg
     4921 aaacaacaga aaacagcgct gaacatggct aaattcatcc agaatcagtc tctgctcctg
     4981 ctggagaagc ttaacgagct tgatcttgat gtagaggccg acctttgtga gaaactccac
     5041 gatgatgcgg agcatctttt ccgtacactg tcttcgggac tggatgaatt acaggatgga
     5101 aactgatggc cagagtgtct attagtgaag cggctcggct tgttaaggtt agtcgcccca
     5161 caatttacaa gatgattaac agtgggaaac taagttacac ctctgttgta aagcatggga
     5221 aaaatactaa agttgttgat acatcagagc ttattagggt ttttggctcg ctttatggtg
     5281 ggggcgacac tgtaaaacat gatgtaaaag gtgacgcagg cgctacaggg gttaacatct
     5341 ctggtttaca tgatttacag cataaaattg ctttgcttga agctgaaaac gatggtctta
     5401 aaggggctgt taaagctcgt gatgaacata tcgattctct tcgccaggct atgcagcttc
     5461 tggaacacaa gctcgaaccc tctccacagg ttcattctcc gtggtggaaa ttctggaaga
     5521 aatcctgacc ccgtcatgct tcagttttcc tctccggcca ttccggggag ttcctgaacc
     5581 ttcccaaaaa tcgccagatc gctgaacgct cctcaccact aaaaatatcc acaccccacg
     5641 caaaattaaa attttgcaga tttgtatttt atatcagtag tttacgttct ataaatttcg
     5701 tttttttaat tttatttata cagatccgac tctatgacga ctcgtcatag agtccccggc
     5761 gagtcgtcat agagtcggta acgactcagg actataaaaa caataagtta acaacgaccc
     5821 gactctagac ccgactcccc gtttggttgt tttttagacg tgacgactct gggtacgact
     5881 ctagtgaaga gtcgccggtg aatcgtgtgg tgagtcgtcc ggcgacttgc cataacgtga
     5941 ttgccggccg tcgacgaacc cgattcctga acgtgctgaa aaccggtttt ccggctgcgc
     6001 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     6061 gaacgccgga aacaggactt accctggctg aatagcgtga attttcgcgc tatgcacttg
     6121 cgtgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     6181 ggtttgttgc ctattttacc ggtttcccgt cagaaacgcc ctgaggccgt tttagcggtg
     6241 cgtacaattt tggtgttatg ttaaatcatg gccacaaaat tccataaagt gaccaatgta
     6301 atgttaaatg agcattgtgg atgaaagatg ttgttttatg gtattatttt ttatgttcta
     6361 aacttaaatt tatttctatc ggggtattat aatgagcaat aaagattctt tagaaagaaa
     6421 agtaacacca agaaaaaatg aacctgtatt cccgatggct ctaagagttg ggatagctga
     6481 agatatttgt tttttagagt tagtggactt tcctgaaaaa gaatcaggaa gggtttttta
     6541 ttccatagct ctaaccaaaa aacatgcaaa gaaccttatc gatggattga ataaattcgt
     6601 tgagtcaggt gattagtatg aaatacatta accactgcga tagtgaagat tattatatta
     6661 ctaatgatag cattgatctc ggtgttcgtt atgatgtttc tgattataat aagttagagc
     6721 taaatgattt aagagatccg gatttgttta aagttttggg tcgagcattt gttgttgttg
     6781 agaattctgt ttgggagtct tggaatgatg atgttaaaac attagattgc tctggtgacg
     6841 gttatgagtc aattttaaaa cattacgaat cattagaatt atcaggtgaa ttcttattgt
     6901 tttttaaaaa aataagaagt gccatttctg tttataatag agaatacgag ttctgcactg
     6961 gtgacctaga accatctttg caggctattg ttcagttgat taggtatgcc cctgaacttg
     7021 ctgcaaaaaa agacgctcag atttactttg ataaaaaatc agagtgtttt gggcttatat
     7081 ataaacaaac taagaaaagc aaaggcacgc taaatttaat agtgaaaaca aataacgaga
     7141 ttattttttc ctttgttaga aaagtttctg gagtggttaa ggttacaggt agggcatata
     7201 ttggcgatag ccttgataac tccattgcta tcaaatcatt atttaaaatg atggagtggt
     7261 gatgactgtg ttatcaactt taaataacct caaatcaatc ccttgtatta atgcatttac
     7321 gggcagagca aataattcat atttatggcg ttatgttatg gaagatcata aagatgagtt
     7381 tggcgctaca actattaagt gtttttatct tagggaggaa atttcagagg attatgtgtc
     7441 tatttatcat tcaaataaag attattatga aggaatggta agggatgtac ataaaagaat
     7501 ccctgtaggg aaaaccaaac ctgccatata tctctcattt gatgcagcgg aagtgatcga
     7561 gcaagttaac aaaccagata aatgtattga tataaaggac gaaggttacc ctcacttggg
     7621 tttgtggtat ttatttgata ttaatgatga atttactttg attgaggtga ctacaactat
     7681 tttggaatta tcctcttttt ttcaaagaaa tgaaggtggc gtggttgttc ctctttagcg
     7741 aaaaccgtct tgacgtcatc aagtgtgacc tagtactctt cttgaaaaag aaaaaccgcc
     7801 tttgcggggc ggtttttctt aggttctaag agtttgcggc tctatgaacc aaggtaactg
     7861 gctagttaga ggagcgcagt aaccaaaatc gttctttcag tttagcctta actggcacat
     7921 aacttcaaga ctaactcctc taaatcagtt accagtggct gctgccagtg gcgcttcgtc
     7981 gtgtctttcc gggttggact caagttgata gttaccggaa aaggcgcagc agtcggactg
     8041 aacggggggt tcgtgcatac agtccagctt ggagcgaact gcctacccgg aactgagtgt
     8101 caggcgtgga atgagataaa cgcggccata acagcggaat gacaccggta aaccgaaagg
     8161 caggaacagg agagcgcacg agggagccac cagggggaaa cgcctggtat ctttatagtc
     8221 ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg tcaggggggc
     8281 ggagcctatg gaaaaacggc cgctgtgcgg cctctccttc tccccatcac gcgatcgctc
     8341 ggttttctcg agcttttata cgcatgctcc tgccgctcgc cgcagccgaa cggcaggagc
     8401 gtagcgactg agtgagcgag gaagcggaaa agagaacatc cctacactga gcactgacgc
     8461 tctgaggcct cttatgacga aacaacagaa aacagcgctg aacatggcta aattcatcca
     8521 gaatcagtct ctgctcctgc tggagaagct taacgagctt gatcttgatg tagaggccga
     8581 cctttgtgag aaactccacg atgatgcgga gcatcttttc cgtacactgt cttcgggact
     8641 ggatgaatta caggatggaa actgatggcc agagtgtcta ttagtgaagc ggctcggctt
     8701 gttaaggtta gtcgccccac aatttacaag atgattaaca gtgggaaact aagttacacc
     8761 tctgttgtaa agcatgggaa aaatactaaa gttgttgata catcagagct tattagggtt
     8821 tttggctcgc tttatggtgg gggcgacact gtaaaacatg atgtaaaagg tgacgcaggc
     8881 gctacagggg ttaacatctc tggtttacat gatttacagc ataaaattgc tttgcttgaa
     8941 gctgaaaacg atggtcttaa aggggctgtt aaagctcgtg atgaacatat cgattctctt
     9001 cgccaggcta tgcagcttct ggaacacaag ctcgaaccct ctccacaggt tcattctccg
     9061 tggtggaaat tctggaagaa atcctgaccc cgtcatgctt cagttttcct ctccggccat
     9121 tccggggagt tcctgaacct tcccaaaaat cgccagatcg ctgaacgctc ctcaccacta
     9181 aaaatatcca caccccacgc aaaattaaaa ttttgcagat ttgtatttta tatcagtagt
     9241 ttacgttcta taaatttcgt ttttttaatt ttatttatac agatccgact ctatgacgac
     9301 tcgtcataga gtccccggcg agtcgtcata gagtcggtaa cgactcagga ctataaaaac
     9361 aataagttaa caacgacccg actctagacc cgactccccg tttggttgtt ttttagacgt
     9421 gacgactctg ggtacgactc tagtgaagag tcgccggtga atcgtgtggt gagtcgtccg
     9481 gcgacttgcc ataacgtgat tgccggccgt cgacgaaccc gattcctgaa cgtgctgaaa
     9541 accggttttc cggctgcgcc ggcaacgctt cacggccacg caataaattg cgtccccgtt
     9601 caccgttttc ggatgggaag aacgccggaa acaggactta ccctggctga atagcgtgaa
     9661 ttttcgcgct atgcacttgc gtgcatactc atgcatgccg taaaaacaga gcctgcgcgt
     9721 ttctggcggg ttttcgggtg gtttgttgcc tattttaccg gtttcccgtc agaaacgccc
     9781 tgaggccgtt ttagcggtgc gtacaatttt ggtgttatgt taaatcatgg ccacaaaatt
     9841 ccataaagtg accaatgtaa tgttaaatga gcattgtgga tgaaagatgt tgttttatgg
     9901 tattattttt tatgttctaa acttaaattt atttctatcg gggtattata atgagcaata
     9961 aagattcttt agaaagaaaa gtaacaccaa gaaaaaatga acctgtattc ccgatggctc
    10021 taagagttgg gatagctgaa gatatttgtt ttttagagtt agtggacttt cctgaaaaag
    10081 aatcaggaag ggttttttat tccatagctc taaccaaaaa acatgcaaag aaccttatcg
    10141 atggattgaa taaattcgtt gagtcaggtg attagtatga aatacattaa ccactgcgat
    10201 agtgaagatt attatattac taatgatagc attgatctcg gtgttcgtta tgatgtttct
    10261 gattataata agttagagct aaatgattta agagatccgg atttgtttaa agttttgggt
    10321 cgagcatttg ttgttgttga gaattctgtt tgggagtctt ggaatgatga tgttaaaaca
    10381 ttagattgct ctggtgacgg ttatgagtca attttaaaac attacgaatc attagaatta
    10441 tcaggtgaat tcttattgtt ttttaaaaaa ataagaagtg ccatttctgt ttataataga
    10501 gaatacgagt tctgcactgg tgacctagaa ccatctttgc aggctattgt tcagttgatt
    10561 aggtatgccc ctgaacttgc tgcaaaaaaa gacgctcaga tttactttga taaaaaatca
    10621 gagtgttttg ggcttatata taaacaaact aagaaaagca aaggcacgct aaattta
//
