LOCUS       NZ_CP139690            10046 bp    DNA     circular CON 08-MAY-2024
DEFINITION  Klebsiella pneumoniae strain P6 plasmid p6Co, complete sequence.
ACCESSION   NZ_CP139690
VERSION     NZ_CP139690.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38111789
            Assembly: GCF_034341085.2
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Tian,D.
  TITLE     The evolution and transmission of ST65 Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-APR-2024) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Jining, Shandong
            272000, China
  REMARK    Sequence update by submitter
REFERENCE   3  (bases 1 to 10046)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2024) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Jining, Shandong
            272000, China
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   4  (bases 1 to 10046)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Jining, Shandong
            272000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_034341085.2-RS_2024_05_07
            Annotation Date                   :: 05/07/2024 08:11:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,450
            CDSs (total)                      :: 5,330
            Genes (coding)                    :: 5,173
            CDSs (with protein)               :: 5,173
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 71 of 157
            Pseudo Genes (incomplete)         :: 88 of 157
            Pseudo Genes (internal stop)      :: 34 of 157
            Pseudo Genes (multiple problems)  :: 34 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139690.2.
            On May 8, 2024 this sequence version replaced NZ_CP139690.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="P6"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p6Co"
                     /geo_loc_name="China: Shandong"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2018-01-30"
                     /collected_by="Dongxing Tian"
     gene            1..1689
                     /locus_tag="UMY64_RS27200"
                     /old_locus_tag="UMY64_27255"
     CDS             1..1689
                     /locus_tag="UMY64_RS27200"
                     /old_locus_tag="UMY64_27255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_020956933.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGVKDYGHDYHPAPKTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKVY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            1699..1956
                     /locus_tag="UMY64_RS27205"
                     /old_locus_tag="UMY64_27260"
     CDS             1699..1956
                     /locus_tag="UMY64_RS27205"
                     /old_locus_tag="UMY64_27260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_041937905.1"
                     /translation="MGLKLNLTWFDKKTEEFIGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVEKPWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            2041..2190
                     /locus_tag="UMY64_RS27210"
                     /old_locus_tag="UMY64_27265"
     CDS             2041..2190
                     /locus_tag="UMY64_RS27210"
                     /old_locus_tag="UMY64_27265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_020956931.1"
                     /translation="MKKVKVVFLFILIFLGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            3674..5635
                     /locus_tag="UMY64_RS27215"
                     /old_locus_tag="UMY64_27270"
     CDS             3674..5635
                     /locus_tag="UMY64_RS27215"
                     /old_locus_tag="UMY64_27270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_020956928.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRVRRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGITWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGQQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            5635..6366
                     /gene="mobC"
                     /locus_tag="UMY64_RS27220"
                     /old_locus_tag="UMY64_27275"
     CDS             5635..6366
                     /gene="mobC"
                     /locus_tag="UMY64_RS27220"
                     /old_locus_tag="UMY64_27275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_020956927.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQISVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6373..6903
                     /locus_tag="UMY64_RS27225"
                     /old_locus_tag="UMY64_27330"
     CDS             6373..6903
                     /locus_tag="UMY64_RS27225"
                     /old_locus_tag="UMY64_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956926.1"
                     /translation="MALYMVFIEDVEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRTQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKSARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(6931..7110)
                     /locus_tag="UMY64_RS27230"
                     /old_locus_tag="UMY64_27290"
     CDS             complement(6931..7110)
                     /locus_tag="UMY64_RS27230"
                     /old_locus_tag="UMY64_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7179..7556
                     /locus_tag="UMY64_RS27235"
                     /old_locus_tag="UMY64_27295"
     CDS             7179..7556
                     /locus_tag="UMY64_RS27235"
                     /old_locus_tag="UMY64_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146445.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(7618..7917)
                     /locus_tag="UMY64_RS27240"
                     /old_locus_tag="UMY64_27300"
     CDS             complement(7618..7917)
                     /locus_tag="UMY64_RS27240"
                     /old_locus_tag="UMY64_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023389908.1"
                     /translation="MPYDVEWKQGAIEDVTMLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(7907..8170)
                     /locus_tag="UMY64_RS27245"
                     /old_locus_tag="UMY64_27305"
     CDS             complement(7907..8170)
                     /locus_tag="UMY64_RS27245"
                     /old_locus_tag="UMY64_27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146443.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESDPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            8944..9462
                     /locus_tag="UMY64_RS27250"
                     /old_locus_tag="UMY64_27335"
     CDS             8944..9462
                     /locus_tag="UMY64_RS27250"
                     /old_locus_tag="UMY64_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956924.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVAQSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     BGC             1..6689
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(2294..2872)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          5636..6363
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="100.0"
                     /evalue="9.930000000000001e-144"
ORIGIN
        1 atgagtggtg gagacggtcg aggtccgggt aattcaggtc tgggacataa tggtggtcag
       61 gccagtggga atgtgaacgg tacgtctggt aaaggtggcc cttcatcagg tggtggtacg
      121 gatccaaaca gcgggccggg ctggggtacg acgcatacgc cgaatggaga tattcataac
      181 tataatccgg gggagtttgg taacggcggg agtaaacccg gtggtaatgg tggtaacagc
      241 ggcaatcata gcggtagctc tggtggcgga cagtcttcgg ccactgcgat ggctttcggt
      301 ctgcctgccc tggctactcc gggggctgaa ggactggctt tatcagtttc cggcgatgcg
      361 ttgtcggccg ccgttgctga tgtgctggct gccctgaagg ggccgtttaa gtttggtctg
      421 tgggggattg cgatctacgg tgtgctgcct tctgagattg caaaagatga tccgaaaatg
      481 atgtcaaaaa ttatgacgtc attaccggcc gatgcggtga cggagactcc ggcaagtact
      541 ttaccactgg accaggcgac ggttcgtgtc agacaacggg ttgtggatgt ggtgaaggat
      601 gagcggcagc atattgcggt tgtcgcaggt cggccaatga gtgtccctgt ggtggatgcg
      661 aaaccgacaa aacgtccggg ggtattcagt gtgtcgattc cgggtctccc gtctctgcag
      721 gtgagcgtac cgaaaggtgt tccgacagcg aaagccccgc caaaaggcat tgttgctgaa
      781 aaaggtgatt cacgtccggc tggttttaca gccggtggta actcccgtga ggccgttatt
      841 cgtttcccga aagagacagg acagaagccg gtttatgtgt cggtgacaga tgttcttacc
      901 ccggcacagg taaaacagcg tcaggaggaa gaaaagcgtc gccagcaggc atgggacgcc
      961 gctcatccgg aagaggggct gaaaagagac tatgataaag cgaaagctga gctggatgcc
     1021 gaagataaaa atattgcgac cttaaacagc cgcattgcat cgacagagaa ggcgctcccc
     1081 ggtgcaaggg ctgctgttca ggaagccgat aaaaaggtaa aagaggcaga ggcaaataaa
     1141 gatgattttg tgacttataa cccgcctcat gaatatggct ccgggtggca ggatcaggtt
     1201 cgctatcttg ataaggatat tcagaatcag aatgagaaat taaaagcggc tcaggcatct
     1261 ttaaacgcaa tgaatgaatc cttatccaga gataaggctg cacttaccgg ggcgatggag
     1321 agccggaaac aaaaggagaa aaaagcgaag gatgcagaaa ataagttaaa tgaggaaaag
     1381 aacaagcctc gcaagggagt taaagattac ggtcatgatt atcatcccgc tccgaagacc
     1441 gaagaaatca aggggttggg ggaattaaaa aaagcaccta aaaaaacacc taaacaaggt
     1501 ggtggtggta gacgtgaccg ttggattggt gataaaggcc gtaaggttta tgaatgggac
     1561 tcccagcacg gtgagcttga agggtatcgt gccagtgatg gcgaacacct cggggcattt
     1621 gatccaaaaa cgggtaagca aattaaaggt ccggatccga aaggacgaaa cattaaaaaa
     1681 tatctttgag aggtaagtat ggggcttaaa ttaaatttaa cctggtttga taagaagact
     1741 gaggaattta ttggtgagga gtattctaaa gactttggtg atgatggttc tgtcattgaa
     1801 agccttggaa tgcctttaaa ggataatatt aacaatggcg ggtttgatgt tgagaaacca
     1861 tgggttccgt tgttgcagcc atattttaaa aataaaattg aagtcgataa gtactggtat
     1921 caaatttcat ttgattatcg ggacggtaac tggtaacaaa aaattatcaa ggatgatttg
     1981 aaatacagac ttccggtgtt cagggatgaa tgccggggtt tttaaataca gggaaggtat
     2041 atgaaaaagg taaaggttgt ttttcttttt atattgattt ttttaggctt tcttctggtc
     2101 gcatgtcagg caaactatat ccgggatgtt cagggtggaa cggtggcacc atcgtcctcc
     2161 tctgaactga cggggatcgc ggttcagtag aaaagatcaa aggatcttct tgagatcctt
     2221 tttttctgcg cgtaatcttt tgccctgtaa acgaaaaaac cacctgggga ggtggtttga
     2281 tcgaaggtta agtcagttgg ggaactgctt aaccgtggta actggctttc gcagagcaca
     2341 gcaaccaaat ctgtccttcc agtgtagccg gactttggtg cacacttcaa gagcaaccgc
     2401 gtgtttagct aaacaaatcc tctgcgaact cccagttacc aatggctgct gccagtggcg
     2461 ttttgcgtgc ttttccgggt tggactcaag tgaacagtta ccggataagg cgcagcagtc
     2521 gggctgaacg gggagttctt gcttacagcc cagcttggag cgaacgacct acaccgagcc
     2581 gagataccag tgtgtgagct atgagaaagc gccacacttc ccgcaaggga gaaaggcgga
     2641 caggtatccg gtaaacggca gggtcggaac aggagagcgc aagagggagc gacctaccgg
     2701 aaacggtggg gatctttaag tcctgtcggg tttcgcccgt actgtcagat tcatggttga
     2761 gcctcacggc tcccacagat gcaccgaaaa agcgtctgtt tatgtgaact ctggcaggag
     2821 ggcggagcct atggaaaaac gccaccggcg cggccctgcc gttttgcctc acatgttagt
     2881 cccctgctta tccacggaat ctgtgggtaa ctttgtatgt gtccgcagcg cccgccgcag
     2941 tctcacgccc ggagcgtagc gaccgagtga gcgaggaggc gcaatttccc cggcacctgc
     3001 aagacggtca ccggacgcgc tgatgtggga tattgtcccc tgattaagtc tttctattcc
     3061 ctgacgatca attatccgaa taggtgaacc atgtcggttt ttcgctgaac attttaagtt
     3121 aacctatctc tgttgctttt ttctccttgc ggagaaatgc aagtcatccc cgctggggcg
     3181 tggctgactt cagggggaaa tcccctgctt acgctggctg gacgccaccg acgcacggtc
     3241 tttccccctc tttctggctc tgtccggctg gacgccggtc acgccagaaa ttcacccggc
     3301 ccccgtcagg ctcacgcctg accacccaca aaaatccacg gtgctgaacg cacctgacga
     3361 agcagcaaag ctgctcctga ttttgcgggt ggtcagccgc tcgctatggc gaactctgac
     3421 gggggccgga acaccggaaa agaccgcact ggggtgccgc cttttcctct cacacgctgg
     3481 ggcgtgctcg ttgttcctcc ggggctgcgc cttttccgca tggggcggaa agaggctggc
     3541 ctccgcggtg aaagctgaac gcaatcacca cggacgtgac cgccgccgcc atgccttggg
     3601 gcatgtccgc ggcttgtgca gcaggggctg cacaacacgc cggtaaaccc gtcgtctgaa
     3661 ggagagagat tgcatgttta atacggattc gcttgcctgg cagtggcata tcccggagct
     3721 ggcccggcag atttacccgt gggtcagctg gctgtaccgg gagtattacg gtctggccgg
     3781 ggcttttctg gttatcggcc tgtttttcgg tctggctgaa ctctgggccg gacggatgcg
     3841 ttttgtgggg ctgagtcata aggagcggcg ggagtatgcc gatcgctata agcgcgataa
     3901 taaccggact gcgccatttt cggtgctggc ctcgctggat tcgcccgggc ggatggtcag
     3961 ggttcggcgg catgtctgga actacgcgta ctggctgacc ggcgctgccg gtctggccct
     4021 tctggggggg cttgttgtcg ccgtgtttat cgagagctgg ggagaaaccg tcctgcgtcc
     4081 ggacctctac gcgaacgcag attattacct gctgcagccg gctaacctgt tcgggattac
     4141 ctggtgcgcg ggcgggctgg ttgtcgggat tgtgttggcg cggctgctgg cgttcctgct
     4201 gcttgacggt cattttgcgg ccgctgacga ggcggtgaac gcgaaactga accaggagag
     4261 ccggcgcagc agccagcgga ccggtgaaat gaccgacgtc cgccacctgc atttcgggga
     4321 gccggtaccg gttaacgcgc tggccgattt ttccacggag caggcccgga aacagcaggc
     4381 ggtgtttctg ggtaaggatg agcagggcca gccggtgctg gtcccgcgtg atacctggcg
     4441 caagaccaac attcagatcc tcggcctgcc cgggagcggc aagagcgtga tggccaccaa
     4501 cgcgctcatt cgctgcgtac gcgatttcgg ggacgcggtg gtgtacttcg acccgaaagg
     4561 ggacgcgtgg gcaccgcacg tcttccgggc gcactgcccg gacttcaccc tgctggatct
     4621 gcggccgggg aaaccggcgc agctgaatct gttccgcgat ctcgaccagt acgccctgaa
     4681 gaatctgctg gttgccgggt ttaacctgag tgagaccggc gacgtggcgg accactaccg
     4741 catcagcgag cagaaagcgg cgaagctgat tgcggagcag ttcccgcagg gggcaaacat
     4801 tcagcaggtg ctggcggcgg catacgcgct gccggaggcc ctgaaaaagg acgtgaaggg
     4861 gctgatcacc aagctggaga acgtggccga cctgagcgtc ctgcagacgg acagcggcat
     4921 cgacgtggcg gggataatca acggcggcgg ctgcctgtac gtcatcggct cgatggacga
     4981 tgaggcggtg atccgggtgc agaagatgtt gtttgcccgc tgcgcgcaaa tcatcattgc
     5041 gcgggatgag ttccggaggt ggccgcacgc gagcatcatg ctcgatgaga ttaaatatct
     5101 gctgtcgaaa tacgtgctga acgcgctggg gacgctgcgc agccgggact gcaacctgct
     5161 gctcgcgcac cagtcgctgg gcgacttcgg ccagtgcggc caggatctgc ccgctgattt
     5221 tgtgaaaacc acggtactgg ataacacgcc gatccgctgg ttctatcgcg cggccagcca
     5281 ggagtccgcg cagtgggcgg cgggccagac cggggagatc cgggtggacg tggagcgccg
     5341 ccgggccagc cgggaggcgg ggaacgtgga gcacatcagc ggcgacagct tcatccagaa
     5401 ggaggcgcgg ccgctgtttg acgtgaacac cctgcaacat ctgcctgacg ggttcgcggt
     5461 gatgaccggg ctgggcgtgg cgcggctggc gttcagcagc ccgctgcggg ttgatcgccg
     5521 ggaaattccg ctgaaaacct tcccggtgct ggcgaaaacg gacccgctgg cggagtacca
     5581 gtcggaggcc gggcagcagc ggccgggtga tgatgatttt gcggggctgt actgatggca
     5641 ctggagcgat acaacgtcag ccacgcgaaa cggcaggcgc ggaacgcgga gaaaacacgg
     5701 ctgaccctgc gctggctgcg ggaagagctg tgttccacgg cggagctggt tgcgcgacgg
     5761 ctggggattg cggcggtaca gccggtttac cgcttcctgg acagcctggt ggcgaaaggg
     5821 ctgctggtcc gggcgaaata cccggttgac ggccgtcaga tcagcgtgtg ggggctgacg
     5881 ccgcacgggg tggcgttctc gttcgacgag gacgagccgc tgacggacat tattccgttt
     5941 cagccgtcgc gggtgtcggc ggcgcagctg ccgcaccggc tggccgtgca gtccctgcgc
     6001 ctcgccatgg aggcccgggg ggccaccggc tggcggtacc tgcaccgtat tgccctgaaa
     6061 gggatgaagg tgccggacgc gctggcggag ctggacggca gaacggtggc gtttgaagtg
     6121 gagcgcaccg tgaagagccg caggcgctat caggaggtgg tgtccgggta tctgtttaac
     6181 cgccgggcga acggcatcga tgaaatctgg tacatctgcc cggaccgggc gacgcaggtc
     6241 agggtgcagc gggcgatcct gtcggtggat gaaatcgtga atccacagac cggcgaggcc
     6301 cgcaaaacgg cggagctaga tcgtgaacgg ctgtttgcgt gctttaaatt tatgacaacg
     6361 gagtgaggga ttatggcgct gtacatggta tttattgaag atgtggagga ccgggacggc
     6421 gagcgctgca tcatgccgac cagcgagcat cttgaagata tggtggtgct cgcctggcag
     6481 accggggcgt atgttttctg ttttgtcgac cggacccagc atctggcgat gatgaaatcc
     6541 atcgggatgt atgacgagcg gctcaacagc gacgaaaaag ccacatactt tttcaccggt
     6601 gaaaatccgc aatgggaacg ggacaaaccc gcaggaatga agctgtgtga ttacctctat
     6661 cgcaaggtgg agcggttcgc catcgagaac ggtgtgcagg tggttgatgc cgggaagtgc
     6721 tcaccggagc agattagcgc gatgattgag gtggtcagcg agcggcacgg ggaaagcgcg
     6781 gtgaacgaga agctgaaagc ggagctgatg cgggaatata acagccgcta ttttaacgtg
     6841 ccggtgcctg aactgccgga ggacaaatcc gcccgccggt ggtggccgtt cggcaaaaaa
     6901 taaacgccag caccggccat gccggtgctg ttattcctcc ccgagcttat cccgcatcac
     6961 ctgccagagt gtttccgcat cctcgtgcag ccgctcgcac atgtcggccg gggtatccat
     7021 atccagctcg ttcagtttct cgagcaacag cagcgactgg tccttaatga atttagccat
     7081 attgagggca gttttttcct gttttgtcat gattctttcc ttccgggcat ggtgtgcgcc
     7141 tgtcgggtat actgtctcaa aaatagcgga gtgagacaat ggcgtgggtg actgtgagac
     7201 aggcgacgga actgacgggg aaagcccgga gttccctgta tcgggatatg gcgaaaggcc
     7261 gcgtatcata ccgtacggaa gctgatggtg gtcgggtggt ggataccagc gagcttatcc
     7321 gggtatacgg tgagttaaga cagggtgaga ctcacgagcg ggacgggttg agactgccgg
     7381 atgagactga aaaaacggcc tccgacgcgt tgatcgcgga gataaaagca ctcagggagg
     7441 aggtggccgg actgcggcag gaaatgcagg ccatgagact gctggaacac aaaccggaga
     7501 cagcagagac accggccgga acaccgcgcc ggtggtggca gtggggaaaa cgctgacgcg
     7561 ctgtgcttgt ccaaccggcg acggccacgc aatgaattgc gcccccgtca ccggttatca
     7621 gcgaacggta tggggattct ggcgctggcc aaccacggcc agaacccgga ctttcctggt
     7681 ctggtcatca acatcataca ggacatgctg tcccatcaga ctgatccggc gaacgtcctc
     7741 gccatagcgt gagtccactt catagaggcg cgggaagtcg gccagtttgt ccgccgcagt
     7801 gagaacgcga tcggtaacgt tctgcgcatc ccacaaggat gaattttccg cgatatagtc
     7861 aaacagcatc gttacatcct cgatggctcc ctgtttccat tccacatcat acggcatcgc
     7921 gcagttcctg ataacggcca tgcaggcgct ggcggagttg ttccgtggtc atgcccggac
     7981 gcgtgtcagc acgggcggcg gttactttgg cgcgaacctg atcttcatac tctgcctggg
     8041 ccagttcttc gttagccagg cgttgcaaca acgcagcttt gtccgggtca ctttccggca
     8101 gtatgcgggg caggttttca gccagttccc gcaggaagtt ggcgttttcg tagtgtgtag
     8161 tgttgctcat tgcggaggct cccggattaa tgcggtgata tgaagaagtt tagcaggggg
     8221 cggtctgaaa gaatatggtc gtgtcagtca ggctgatgaa gcagaaagtc tgtaaaaaaa
     8281 gggcttacgg catccatttt acgtcaaaac atatgctatg cagttgcgtg cataggcatg
     8341 cattaggata aagtttaccg ggcgcgtttc cggcggtttt ccgggtgatt tgttgcctgt
     8401 tttatcccgt agccgccgga aacgctctga gggcttctcg ggcggtgcgg gcaacgggtt
     8461 ttatggtaaa tggctggtta tgaggcattc agctgacgat ttttgcgtca acttttcgct
     8521 gtatgtttaa acatgagttt aacattagcg tcatctgata gcgctgttgc taatttcatc
     8581 gaccagacag gtaatttcct tgtaaatcaa gcgcgaaaga tcagcaaaaa agctatgtgt
     8641 gcgcttatgg aataaggtag aataagggat tctgatgatc gtgaggttgt cagtgaaaaa
     8701 cgaggcagct atcagatctt cggcctgaga aactaaggat tctgatagct gccggtgact
     8761 cagttcgtga aaactgaatc ttacctgatc aacaaacgcc cgattagggc gtttgtcaag
     8821 tggtgctgca ttatgtagta acttggtacc gacttgccag gcatttttca cagttttttg
     8881 atgtgcgaat gctcatccgg acaccgtcac ctgttattta tcctctgtaa acaggtgcta
     8941 taaatgcaaa aagtaactga tctgtatcct cctgaaattg ctcgccataa gctccagaat
     9001 cacttcgccg gtaataccgt tatgctggag ttaatccaga aactaaataa aaccagcctg
     9061 tgtacttttg cggcgctctg tgacggcaac gtagtgacca cttccggcta taacatcacg
     9121 gccgatctat gtgttaaccg tgcgtcagcc gtggcccatt cgcttaagca aaaatatctg
     9181 ccagttacgg ccagaacgat ttcgacaaag gctgatgtag gtggtgcggt aaagcaggcg
     9241 gctttctgta tcgatgagga cgatcttgca aggctgaaat cagagccgga aaagatgatg
     9301 aaagagtgcg aacgtaacct gaacagccag aagcgcacca atgctcagaa ggaaatgagc
     9361 cggttgtaca aggagtttgg tgaagacggc atactggcgc tgttacgcaa tgttgcccag
     9421 tcgaacggta caccgccgcc aggcgcacac cctgcaagtt gataccagta aagcccctaa
     9481 ggggctttat ttttaaggtt ttctggatgc cgccagctct tgatctgtca gctaatcgcg
     9541 gtgcagatag ttcccttcta ccggcggtgg cgttaccagt tttgcttttc cttctttcgg
     9601 acgagaattt cactcttacc gtacattttc gtacggttgg acctcgaacc cctttcaggc
     9661 ccatggccag tgcctgagcg acaaaggcgt ccgccaggcg cttttcttca ggtgcgtcat
     9721 ggtggcctcc gggttgtctc cgattcctga acagtttaac cgcaggtact ctcctcatgc
     9781 catttcgctt tcaggtacat ctataccaat ataaaagtaa gtgtgatgaa tatctcaaat
     9841 aataaaattt gttgccatga gaaattttcg ggaaaagacg cgatctgctt acaggcggct
     9901 ccgggcgctt ttttgtgcct gaagatgaga actaatctca tatattgaca cctgaaaact
     9961 ggaggagtaa ggtaacaatt atactgtgta tatatacagt atattggttt taatttattg
    10021 ttttaaatgt caaagaggaa aacgat
//
