LOCUS       NZ_CP139668            10046 bp    DNA     circular CON 11-MAY-2024
DEFINITION  Escherichia coli strain TP10-1 plasmid p3, complete sequence.
ACCESSION   NZ_CP139668
VERSION     NZ_CP139668.2
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN38111794
            Assembly: GCF_034336645.2
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Tian,D.
  TITLE     The evolution and transmission of ST65 Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10046)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-APR-2024) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Jining, Shandong
            272000, China
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 10046)
  AUTHORS   Tian,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2023) Clinical Laboratory, Affiliated Hospital of
            Jining Medical University, No. 89, Guhuai Road, Jining, Shandong
            272000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 2016
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 205x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_034336645.2-RS_2024_05_11
            Annotation Date                   :: 05/11/2024 01:58:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,981
            CDSs (total)                      :: 4,862
            Genes (coding)                    :: 4,622
            CDSs (with protein)               :: 4,622
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 240
            CDSs (without protein)            :: 240
            Pseudo Genes (ambiguous residues) :: 0 of 240
            Pseudo Genes (frameshifted)       :: 75 of 240
            Pseudo Genes (incomplete)         :: 174 of 240
            Pseudo Genes (internal stop)      :: 46 of 240
            Pseudo Genes (multiple problems)  :: 54 of 240
            Pseudo Genes (short protein)      :: 4 of 240
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139668.2.
            On May 11, 2024 this sequence version replaced NZ_CP139668.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TP10-1"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p3"
                     /geo_loc_name="China: Shandong"
                     /lat_lon="31.23 N 121.45 E"
                     /collection_date="2023-02-25"
                     /collected_by="Dongxing Tian"
     gene            join(9794..10046,1..11)
                     /locus_tag="UMY61_RS24855"
                     /old_locus_tag="UMY61_24890"
     CDS             join(9794..10046,1..11)
                     /locus_tag="UMY61_RS24855"
                     /old_locus_tag="UMY61_24890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146443.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESDPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            1..300
                     /locus_tag="UMY61_RS24860"
                     /old_locus_tag="UMY61_24885"
     CDS             1..300
                     /locus_tag="UMY61_RS24860"
                     /old_locus_tag="UMY61_24885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_023389908.1"
                     /translation="MPYDVEWKQGAIEDVTMLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(362..739)
                     /locus_tag="UMY61_RS24865"
                     /old_locus_tag="UMY61_24880"
     CDS             complement(362..739)
                     /locus_tag="UMY61_RS24865"
                     /old_locus_tag="UMY61_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004146445.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="hypothetical protein"
     gene            808..987
                     /locus_tag="UMY61_RS24870"
                     /old_locus_tag="UMY61_24875"
     CDS             808..987
                     /locus_tag="UMY61_RS24870"
                     /old_locus_tag="UMY61_24875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1015..1545)
                     /locus_tag="UMY61_RS24875"
                     /old_locus_tag="UMY61_24870"
     CDS             complement(1015..1545)
                     /locus_tag="UMY61_RS24875"
                     /old_locus_tag="UMY61_24870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956926.1"
                     /translation="MALYMVFIEDVEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRTQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKSARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(1552..2283)
                     /gene="mobC"
                     /locus_tag="UMY61_RS24880"
                     /old_locus_tag="UMY61_24865"
     CDS             complement(1552..2283)
                     /gene="mobC"
                     /locus_tag="UMY61_RS24880"
                     /old_locus_tag="UMY61_24865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_020956927.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQISVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(2283..4244)
                     /locus_tag="UMY61_RS24885"
                     /old_locus_tag="UMY61_24860"
     CDS             complement(2283..4244)
                     /locus_tag="UMY61_RS24885"
                     /old_locus_tag="UMY61_24860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_020956928.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRVRRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGITWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGQQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(5728..5877)
                     /locus_tag="UMY61_RS24890"
                     /old_locus_tag="UMY61_24855"
     CDS             complement(5728..5877)
                     /locus_tag="UMY61_RS24890"
                     /old_locus_tag="UMY61_24855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_020956931.1"
                     /translation="MKKVKVVFLFILIFLGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5962..6219)
                     /locus_tag="UMY61_RS24895"
                     /old_locus_tag="UMY61_24850"
     CDS             complement(5962..6219)
                     /locus_tag="UMY61_RS24895"
                     /old_locus_tag="UMY61_24850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cloacin immunity family protein"
                     /protein_id="WP_041937905.1"
                     /translation="MGLKLNLTWFDKKTEEFIGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGGFDVEKPWVPLLQPYFKNKIEVDKYWYQISFDYRDGNW"
                     /name="cloacin immunity family protein"
     gene            complement(6229..7917)
                     /locus_tag="UMY61_RS24900"
                     /old_locus_tag="UMY61_24845"
     CDS             complement(6229..7917)
                     /locus_tag="UMY61_RS24900"
                     /old_locus_tag="UMY61_24845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_020956933.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGVKDYGHDYHPAPKTEEIKGLGELKKAPKKTPKQGGGGRRDRWIGDKGRKVY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQIKGPDPKGRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(8502..9020)
                     /locus_tag="UMY61_RS24905"
                     /old_locus_tag="UMY61_24895"
     CDS             complement(8502..9020)
                     /locus_tag="UMY61_RS24905"
                     /old_locus_tag="UMY61_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_020956924.1"
                     /translation="MQKVTDLYPPEIARHKLQNHFAGNTVMLELIQKLNKTSLCTFAAL
                     CDGNVVTTSGYNITADLCVNRASAVAHSLKQKYLPVTARTISTKADVGGAVKQAAFCID
                     EDDLARLKSEPEKMMKECERNLNSQKRTNAQKEMSRLYKEFGEDGILALLRNVAQSNGT
                     PPPGAHPAS"
                     /name="hypothetical protein"
     BGC             1229..10046
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          5047..5625
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.454"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1556..2283)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="100.0"
                     /evalue="9.930000000000001e-144"
ORIGIN
        1 atgccgtatg atgtggaatg gaaacaggga gccatcgagg atgtaacgat gctgtttgac
       61 tatatcgcgg aaaattcatc cttgtgggat gcgcagaacg ttaccgatcg cgttctcact
      121 gcggcggaca aactggccga cttcccgcgc ctctatgaag tggactcacg ctatggcgag
      181 gacgttcgcc ggatcagtct gatgggacag catgtcctgt atgatgttga tgaccagacc
      241 aggaaagtcc gggttctggc cgtggttggc cagcgccaga atccccatac cgttcgctga
      301 taaccggtga cgggggcgca attcattgcg tggccgtcgc cggttggaca agcacagcgc
      361 gtcagcgttt tccccactgc caccaccggc gcggtgttcc ggccggtgtc tctgctgtct
      421 ccggtttgtg ttccagcagt ctcatggcct gcatttcctg ccgcagtccg gccacctcct
      481 ccctgagtgc ttttatctcc gcgatcaacg cgtcggaggc cgttttttca gtctcatccg
      541 gcagtctcaa cccgtcccgc tcgtgagtct caccctgtct taactcaccg tatacccgga
      601 taagctcgct ggtatccacc acccgaccac catcagcttc cgtacggtat gatacgcggc
      661 ctttcgccat atcccgatac agggaactcc gggctttccc cgtcagttcc gtcgcctgtc
      721 tcacagtcac ccacgccatt gtctcactcc gctatttttg agacagtata cccgacaggc
      781 gcacaccatg cccggaagga aagaatcatg acaaaacagg aaaaaactgc cctcaatatg
      841 gctaaattca ttaaggacca gtcgctgctg ttgctcgaga aactgaacga gctggatatg
      901 gataccccgg ccgacatgtg cgagcggctg cacgaggatg cggaaacact ctggcaggtg
      961 atgcgggata agctcgggga ggaataacag caccggcatg gccggtgctg gcgtttattt
     1021 tttgccgaac ggccaccacc ggcgggcgga tttgtcctcc ggcagttcag gcaccggcac
     1081 gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc
     1141 gctttccccg tgccgctcgc tgaccacctc aatcatcgcg ctaatctgct ccggtgagca
     1201 cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca ccttgcgata
     1261 gaggtaatca cacagcttca ttcctgcggg tttgtcccgt tcccattgcg gattttcacc
     1321 ggtgaaaaag tatgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga
     1381 tttcatcatc gccagatgct gggtccggtc gacaaaacag aaaacatacg ccccggtctg
     1441 ccaggcgagc accaccatat cttcaagatg ctcgctggtc ggcatgatgc agcgctcgcc
     1501 gtcccggtcc tccacatctt caataaatac catgtacagc gccataatcc ctcactccgt
     1561 tgtcataaat ttaaagcacg caaacagccg ttcacgatct agctccgccg ttttgcgggc
     1621 ctcgccggtc tgtggattca cgatttcatc caccgacagg atcgcccgct gcaccctgac
     1681 ctgcgtcgcc cggtccgggc agatgtacca gatttcatcg atgccgttcg cccggcggtt
     1741 aaacagatac ccggacacca cctcctgata gcgcctgcgg ctcttcacgg tgcgctccac
     1801 ttcaaacgcc accgttctgc cgtccagctc cgccagcgcg tccggcacct tcatcccttt
     1861 cagggcaata cggtgcaggt accgccagcc ggtggccccc cgggcctcca tggcgaggcg
     1921 cagggactgc acggccagcc ggtgcggcag ctgcgccgcc gacacccgcg acggctgaaa
     1981 cggaataatg tccgtcagcg gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt
     2041 cagcccccac acgctgatct gacggccgtc aaccgggtat ttcgcccgga ccagcagccc
     2101 tttcgccacc aggctgtcca ggaagcggta aaccggctgt accgccgcaa tccccagccg
     2161 tcgcgcaacc agctccgccg tggaacacag ctcttcccgc agccagcgca gggtcagccg
     2221 tgttttctcc gcgttccgcg cctgccgttt cgcgtggctg acgttgtatc gctccagtgc
     2281 catcagtaca gccccgcaaa atcatcatca cccggccgct gctgcccggc ctccgactgg
     2341 tactccgcca gcgggtccgt tttcgccagc accgggaagg ttttcagcgg aatttcccgg
     2401 cgatcaaccc gcagcgggct gctgaacgcc agccgcgcca cgcccagccc ggtcatcacc
     2461 gcgaacccgt caggcagatg ttgcagggtg ttcacgtcaa acagcggccg cgcctccttc
     2521 tggatgaagc tgtcgccgct gatgtgctcc acgttccccg cctcccggct ggcccggcgg
     2581 cgctccacgt ccacccggat ctccccggtc tggcccgccg cccactgcgc ggactcctgg
     2641 ctggccgcgc gatagaacca gcggatcggc gtgttatcca gtaccgtggt tttcacaaaa
     2701 tcagcgggca gatcctggcc gcactggccg aagtcgccca gcgactggtg cgcgagcagc
     2761 aggttgcagt cccggctgcg cagcgtcccc agcgcgttca gcacgtattt cgacagcaga
     2821 tatttaatct catcgagcat gatgctcgcg tgcggccacc tccggaactc atcccgcgca
     2881 atgatgattt gcgcgcagcg ggcaaacaac atcttctgca cccggatcac cgcctcatcg
     2941 tccatcgagc cgatgacgta caggcagccg ccgccgttga ttatccccgc cacgtcgatg
     3001 ccgctgtccg tctgcaggac gctcaggtcg gccacgttct ccagcttggt gatcagcccc
     3061 ttcacgtcct ttttcagggc ctccggcagc gcgtatgccg ccgccagcac ctgctgaatg
     3121 tttgccccct gcgggaactg ctccgcaatc agcttcgccg ctttctgctc gctgatgcgg
     3181 tagtggtccg ccacgtcgcc ggtctcactc aggttaaacc cggcaaccag cagattcttc
     3241 agggcgtact ggtcgagatc gcggaacaga ttcagctgcg ccggtttccc cggccgcaga
     3301 tccagcaggg tgaagtccgg gcagtgcgcc cggaagacgt gcggtgccca cgcgtcccct
     3361 ttcgggtcga agtacaccac cgcgtccccg aaatcgcgta cgcagcgaat gagcgcgttg
     3421 gtggccatca cgctcttgcc gctcccgggc aggccgagga tctgaatgtt ggtcttgcgc
     3481 caggtatcac gcgggaccag caccggctgg ccctgctcat ccttacccag aaacaccgcc
     3541 tgctgtttcc gggcctgctc cgtggaaaaa tcggccagcg cgttaaccgg taccggctcc
     3601 ccgaaatgca ggtggcggac gtcggtcatt tcaccggtcc gctggctgct gcgccggctc
     3661 tcctggttca gtttcgcgtt caccgcctcg tcagcggccg caaaatgacc gtcaagcagc
     3721 aggaacgcca gcagccgcgc caacacaatc ccgacaacca gcccgcccgc gcaccaggta
     3781 atcccgaaca ggttagccgg ctgcagcagg taataatctg cgttcgcgta gaggtccgga
     3841 cgcaggacgg tttctcccca gctctcgata aacacggcga caacaagccc ccccagaagg
     3901 gccagaccgg cagcgccggt cagccagtac gcgtagttcc agacatgccg ccgaaccctg
     3961 accatccgcc cgggcgaatc cagcgaggcc agcaccgaaa atggcgcagt ccggttatta
     4021 tcgcgcttat agcgatcggc atactcccgc cgctccttat gactcagccc cacaaaacgc
     4081 atccgtccgg cccagagttc agccagaccg aaaaacaggc cgataaccag aaaagccccg
     4141 gccagaccgt aatactcccg gtacagccag ctgacccacg ggtaaatctg ccgggccagc
     4201 tccgggatat gccactgcca ggcaagcgaa tccgtattaa acatgcaatc tctctccttc
     4261 agacgacggg tttaccggcg tgttgtgcag cccctgctgc acaagccgcg gacatgcccc
     4321 aaggcatggc ggcggcggtc acgtccgtgg tgattgcgtt cagctttcac cgcggaggcc
     4381 agcctctttc cgccccatgc ggaaaaggcg cagccccgga ggaacaacga gcacgcccca
     4441 gcgtgtgaga ggaaaaggcg gcaccccagt gcggtctttt ccggtgttcc ggcccccgtc
     4501 agagttcgcc atagcgagcg gctgaccacc cgcaaaatca ggagcagctt tgctgcttcg
     4561 tcaggtgcgt tcagcaccgt ggatttttgt gggtggtcag gcgtgagcct gacgggggcc
     4621 gggtgaattt ctggcgtgac cggcgtccag ccggacagag ccagaaagag ggggaaagac
     4681 cgtgcgtcgg tggcgtccag ccagcgtaag caggggattt ccccctgaag tcagccacgc
     4741 cccagcgggg atgacttgca tttctccgca aggagaaaaa agcaacagag ataggttaac
     4801 ttaaaatgtt cagcgaaaaa ccgacatggt tcacctattc ggataattga tcgtcaggga
     4861 atagaaagac ttaatcaggg gacaatatcc cacatcagcg cgtccggtga ccgtcttgca
     4921 ggtgccgggg aaattgcgcc tcctcgctca ctcggtcgct acgctccggg cgtgagactg
     4981 cggcgggcgc tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact
     5041 aacatgtgag gcaaaacggc agggccgcgc cggtggcgtt tttccatagg ctccgccctc
     5101 ctgccagagt tcacataaac agacgctttt tcggtgcatc tgtgggagcc gtgaggctca
     5161 accatgaatc tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg
     5221 gtaggtcgct ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgtcc
     5281 gcctttctcc cttgcgggaa gtgtggcgct ttctcatagc tcacacactg gtatctcggc
     5341 tcggtgtagg tcgttcgctc caagctgggc tgtaagcaag aactccccgt tcagcccgac
     5401 tgctgcgcct tatccggtaa ctgttcactt gagtccaacc cggaaaagca cgcaaaacgc
     5461 cactggcagc agccattggt aactgggagt tcgcagagga tttgtttagc taaacacgcg
     5521 gttgctcttg aagtgtgcac caaagtccgg ctacactgga aggacagatt tggttgctgt
     5581 gctctgcgaa agccagttac cacggttaag cagttcccca actgacttaa ccttcgatca
     5641 aaccacctcc ccaggtggtt ttttcgttta cagggcaaaa gattacgcgc agaaaaaaag
     5701 gatctcaaga agatcctttg atcttttcta ctgaaccgcg atccccgtca gttcagagga
     5761 ggacgatggt gccaccgttc caccctgaac atcccggata tagtttgcct gacatgcgac
     5821 cagaagaaag cctaaaaaaa tcaatataaa aagaaaaaca acctttacct ttttcatata
     5881 ccttccctgt atttaaaaac cccggcattc atccctgaac accggaagtc tgtatttcaa
     5941 atcatccttg ataatttttt gttaccagtt accgtcccga taatcaaatg aaatttgata
     6001 ccagtactta tcgacttcaa ttttattttt aaaatatggc tgcaacaacg gaacccatgg
     6061 tttctcaaca tcaaacccgc cattgttaat attatccttt aaaggcattc caaggctttc
     6121 aatgacagaa ccatcatcac caaagtcttt agaatactcc tcaccaataa attcctcagt
     6181 cttcttatca aaccaggtta aatttaattt aagccccata cttacctctc aaagatattt
     6241 tttaatgttt cgtcctttcg gatccggacc tttaatttgc ttacccgttt ttggatcaaa
     6301 tgccccgagg tgttcgccat cactggcacg atacccttca agctcaccgt gctgggagtc
     6361 ccattcataa accttacggc ctttatcacc aatccaacgg tcacgtctac caccaccacc
     6421 ttgtttaggt gtttttttag gtgctttttt taattccccc aaccccttga tttcttcggt
     6481 cttcggagcg ggatgataat catgaccgta atctttaact cccttgcgag gcttgttctt
     6541 ttcctcattt aacttatttt ctgcatcctt cgcttttttc tccttttgtt tccggctctc
     6601 catcgccccg gtaagtgcag ccttatctct ggataaggat tcattcattg cgtttaaaga
     6661 tgcctgagcc gcttttaatt tctcattctg attctgaata tccttatcaa gatagcgaac
     6721 ctgatcctgc cacccggagc catattcatg aggcgggtta taagtcacaa aatcatcttt
     6781 atttgcctct gcctctttta cctttttatc ggcttcctga acagcagccc ttgcaccggg
     6841 gagcgccttc tctgtcgatg caatgcggct gtttaaggtc gcaatatttt tatcttcggc
     6901 atccagctca gctttcgctt tatcatagtc tcttttcagc ccctcttccg gatgagcggc
     6961 gtcccatgcc tgctggcgac gcttttcttc ctcctgacgc tgttttacct gtgccggggt
     7021 aagaacatct gtcaccgaca cataaaccgg cttctgtcct gtctctttcg ggaaacgaat
     7081 aacggcctca cgggagttac caccggctgt aaaaccagcc ggacgtgaat cacctttttc
     7141 agcaacaatg ccttttggcg gggctttcgc tgtcggaaca cctttcggta cgctcacctg
     7201 cagagacggg agacccggaa tcgacacact gaataccccc ggacgttttg tcggtttcgc
     7261 atccaccaca gggacactca ttggccgacc tgcgacaacc gcaatatgct gccgctcatc
     7321 cttcaccaca tccacaaccc gttgtctgac acgaaccgtc gcctggtcca gtggtaaagt
     7381 acttgccgga gtctccgtca ccgcatcggc cggtaatgac gtcataattt ttgacatcat
     7441 tttcggatca tcttttgcaa tctcagaagg cagcacaccg tagatcgcaa tcccccacag
     7501 accaaactta aacggcccct tcagggcagc cagcacatca gcaacggcgg ccgacaacgc
     7561 atcgccggaa actgataaag ccagtccttc agcccccgga gtagccaggg caggcagacc
     7621 gaaagccatc gcagtggccg aagactgtcc gccaccagag ctaccgctat gattgccgct
     7681 gttaccacca ttaccaccgg gtttactccc gccgttacca aactcccccg gattatagtt
     7741 atgaatatct ccattcggcg tatgcgtcgt accccagccc ggcccgctgt ttggatccgt
     7801 accaccacct gatgaagggc cacctttacc agacgtaccg ttcacattcc cactggcctg
     7861 accaccatta tgtcccagac ctgaattacc cggacctcga ccgtctccac cactcatatc
     7921 gttttcctct ttgacattta aaacaataaa ttaaaaccaa tatactgtat atatacacag
     7981 tataattgtt accttactcc tccagttttc aggtgtcaat atatgagatt agttctcatc
     8041 ttcaggcaca aaaaagcgcc cggagccgcc tgtaagcaga tcgcgtcttt tcccgaaaat
     8101 ttctcatggc aacaaatttt attatttgag atattcatca cacttacttt tatattggta
     8161 tagatgtacc tgaaagcgaa atggcatgag gagagtacct gcggttaaac tgttcaggaa
     8221 tcggagacaa cccggaggcc accatgacgc acctgaagaa aagcgcctgg cggacgcctt
     8281 tgtcgctcag gcactggcca tgggcctgaa aggggttcga ggtccaaccg tacgaaaatg
     8341 tacggtaaga gtgaaattct cgtccgaaag aaggaaaagc aaaactggta acgccaccgc
     8401 cggtagaagg gaactatctg caccgcgatt agctgacaga tcaagagctg gcggcatcca
     8461 gaaaacctta aaaataaagc cccttagggg ctttactggt atcaacttgc agggtgtgcg
     8521 cctggcggcg gtgtaccgtt cgactgggca acattgcgta acagcgccag tatgccgtct
     8581 tcaccaaact ccttgtacaa ccggctcatt tccttctgag cattggtgcg cttctggctg
     8641 ttcaggttac gttcgcactc tttcatcatc ttttccggct ctgatttcag ccttgcaaga
     8701 tcgtcctcat cgatacagaa agccgcctgc tttaccgcac cacctacatc agcctttgtc
     8761 gaaatcgttc tggccgtaac tggcagatat ttttgcttaa gcgaatgggc cacggctgac
     8821 gcacggttaa cacatagatc ggccgtgatg ttatagccgg aagtggtcac tacgttgccg
     8881 tcacagagcg ccgcaaaagt acacaggctg gttttattta gtttctggat taactccagc
     8941 ataacggtat taccggcgaa gtgattctgg agcttatggc gagcaatttc aggaggatac
     9001 agatcagtta ctttttgcat ttatagcacc tgtttacaga ggataaataa caggtgacgg
     9061 tgtccggatg agcattcgca catcaaaaaa ctgtgaaaaa tgcctggcaa gtcggtacca
     9121 agttactaca taatgcagca ccacttgaca aacgccctaa tcgggcgttt gttgatcagg
     9181 taagattcag ttttcacgaa ctgagtcacc ggcagctatc agaatcctta gtttctcagg
     9241 ccgaagatct gatagctgcc tcgtttttca ctgacaacct cacgatcatc agaatccctt
     9301 attctacctt attccataag cgcacacata gcttttttgc tgatctttcg cgcttgattt
     9361 acaaggaaat tacctgtctg gtcgatgaaa ttagcaacag cgctatcaga tgacgctaat
     9421 gttaaactca tgtttaaaca tacagcgaaa agttgacgca aaaatcgtca gctgaatgcc
     9481 tcataaccag ccatttacca taaaacccgt tgcccgcacc gcccgagaag ccctcagagc
     9541 gtttccggcg gctacgggat aaaacaggca acaaatcacc cggaaaaccg ccggaaacgc
     9601 gcccggtaaa ctttatccta atgcatgcct atgcacgcaa ctgcatagca tatgttttga
     9661 cgtaaaatgg atgccgtaag cccttttttt acagactttc tgcttcatca gcctgactga
     9721 cacgaccata ttctttcaga ccgccccctg ctaaacttct tcatatcacc gcattaatcc
     9781 gggagcctcc gcaatgagca acactacaca ctacgaaaac gccaacttcc tgcgggaact
     9841 ggctgaaaac ctgccccgca tactgccgga aagtgacccg gacaaagctg cgttgttgca
     9901 acgcctggct aacgaagaac tggcccaggc agagtatgaa gatcaggttc gcgccaaagt
     9961 aaccgccgcc cgtgctgaca cgcgtccggg catgaccacg gaacaactcc gccagcgcct
    10021 gcatggccgt tatcaggaac tgcgcg
//
