LOCUS       NZ_CP139500             5463 bp    DNA     circular CON 13-DEC-2023
DEFINITION  Klebsiella pneumoniae strain 20SC1-3AZ6BT plasmid p3, complete
            sequence.
ACCESSION   NZ_CP139500
VERSION     NZ_CP139500.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN37196594
            Assembly: GCF_034259285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Wang,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-SEP-2023) the College of Veterinary Medicine, China
            Agricultural University, Malianwa, Beijing 100193, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. .0.5.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/13/2023 00:33:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,724
            CDSs (total)                      :: 5,600
            Genes (coding)                    :: 5,435
            CDSs (with protein)               :: 5,435
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 67 of 165
            Pseudo Genes (incomplete)         :: 101 of 165
            Pseudo Genes (internal stop)      :: 36 of 165
            Pseudo Genes (multiple problems)  :: 30 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP139500.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="20SC1-3AZ6BT"
                     /isolation_source="swine"
                     /db_xref="taxon:573"
                     /plasmid="p3"
                     /geo_loc_name="China:Chengdu"
                     /collection_date="2020-10"
                     /collected_by="CAU"
     gene            complement(join(5160..5463,1..95))
                     /locus_tag="REV13_RS28600"
                     /old_locus_tag="REV13_28600"
     CDS             complement(join(5160..5463,1..95))
                     /locus_tag="REV13_RS28600"
                     /old_locus_tag="REV13_28600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001222916.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLLDNEAGLTEQERK
                     LLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTKTG
                     ELCYKKDRILQALKEIKYDLETSPNPDA"
                     /name="hypothetical protein"
     gene            272..1198
                     /locus_tag="REV13_RS28605"
                     /old_locus_tag="REV13_28605"
     CDS             272..1198
                     /locus_tag="REV13_RS28605"
                     /old_locus_tag="REV13_28605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_086356557.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIEGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     PTQQKEQLRELRKRWEVKHNEHMRKNGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGHDIMEWLDTPVKQHELVRRLQHDVARVHGFD
                     MRDRVSRQDRTEPERVKSPSRGFSMGF"
                     /name="MobA/MobL family protein"
     gene            1201..1734
                     /locus_tag="REV13_RS28610"
                     /old_locus_tag="REV13_28610"
     CDS             1201..1734
                     /locus_tag="REV13_RS28610"
                     /old_locus_tag="REV13_28610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000168774.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQNLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVILED
                     KLAEKPVTGKNRKN"
                     /name="hypothetical protein"
     gene            1955..3079
                     /locus_tag="REV13_RS28615"
                     /old_locus_tag="REV13_28615"
     CDS             1955..3079
                     /locus_tag="REV13_RS28615"
                     /old_locus_tag="REV13_28615"
                     /inference="COORDINATES: protein motif:HMM:NF013211.3"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA];
                     GO:0050829 - defense response to Gram-negative bacterium
                     [Evidence IEA]; GO:0031640 - killing of cells of another
                     organism [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="WP_021529456.1"
                     /translation="MSGGDGRGPGNTGLGHNGGKPSGNVNGSSGHGGPSSGGGYHYNPG
                     KGPASTVGPDGEVHINITGGMEKDPPGKHHGNGGSGNGHGAGNHNSNSGGNGKTTSIPG
                     WSPDSGKPGRYLPTNKDKLIKGLIQSIDDHSGTYTIHYTGGAVATVTVPNGDVNKMNVH
                     YNIGSGPSSGNKLTRDTIKSFLEFKAENEANAVNDAISKVNDFYKELASKYGDKLEKEA
                     KALADEAKGKKIRNANEAIKAFDRYKDVLNKKFSVSDREAIAKALESLNKDQMAKQLKI
                     FGKAFGVVGEAIQWGGFISGLVKGFRTGDWNEAFISGEKIAVGKVASVMVTVAFSAMAV
                     NPIGILGFAVIMAVTSALITDERLKQLNSFINGI"
                     /name="colicin-like pore-forming protein"
     gene            3880..4899
                     /locus_tag="REV13_RS28620"
                     /old_locus_tag="REV13_28620"
     CDS             3880..4899
                     /locus_tag="REV13_RS28620"
                     /old_locus_tag="REV13_28620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001271364.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="replication initiation protein"
     Typing          3881..4655
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          273..1195
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aggtcaataa tctgtctctg tcgtgcggtc tgttctccga gcttcctgat gaatccgatc
       61 cgtttcttca gccattcgat gttgctgatg cgcatgttcg tggtgctccc tggtgttttt
      121 ttgcggtgtg ttgtggtcgt gcagtatatc gcttgcggtc ggcgggtgca gggggtatct
      181 tccgtgctaa ttcgtaaaat tagaaggaag cgcacttata tgtttctctc cttcgtcgag
      241 aaacagtgcg ctcctgcgga gggctgaaac cgtggcgacc tatcacctca gcgttaagtt
      301 tggtggcaag ggacaggctg caaaccatgc cgattacatc gagcgcaaag agaaataccg
      361 cgaccgtcaa gatttggaat attccgcaca cggaaacatg ccggaatggg cgcgagataa
      421 tccctcccat ttctggcagg cggctgacca gtttgaacgt gcaaacggtt cgacttatcg
      481 ggaactggag atagcgctgc cccgtgaact gacaccggaa cagcgccttg aactggtgca
      541 ggattttgta cgtcaggaag ctggcgaacg gcatgcatgg agttttgcca ttcacaatcc
      601 gaaagcgagc attgagggag gcgaacagcc tcatgcgcat atcatgatga gtcagcgtgt
      661 gaatgatggc attgaacgta caccggaaca gtatttccgg cgctataacg ccaggtatcc
      721 cgagcgggga ggtgcgaaaa aggattcagg aagcctgact cccactcagc aaaaagagca
      781 gttgcgggaa ttgcgaaaac gctgggaggt gaagcacaac gaacatatga gaaaaaatgg
      841 ctttgaaagg ggcttcattg actgtcggac gctgaaagaa cagggcattg aacgacgtcc
      901 cgaagtgcat ctgggttttg aagctgccgg acggcttgat gatgcccagc gtcatgacat
      961 catgcaaaaa cggtctgaac cggactattc ccgccatctt gaacaggcgc gaacggtcgg
     1021 gcatgacatc atggaatggc tggacactcc ggttaagcaa catgaactgg tcagaaggct
     1081 gcaacatgat gttgcgcggg tgcatggctt tgacatgaga gacagagtga gcagacagga
     1141 ccgaactgaa cctgaacggg ttaaatcacc gtcacgtgga ttttctatgg gattctgaac
     1201 atggccagca cagaagaaaa attactcacg ctcctgttat cggtggagca tttgcaacat
     1261 accgcaatgg agcaacagaa agctgttgct gattctctgg ctgtggcaag ggaaacactt
     1321 cgcggatatc gggatatttc tgacggtgtg gcaaaacagg tcagggatga agtcagaaat
     1381 gaggtgcaga atctggacgt agcagggctg ataggtgagc gcatcagcca ggattttcag
     1441 cggctggaag agtcagtcgc aaacatgaag gcaaacgcgg atgccctgaa cagggatatt
     1501 gcgacaattc acagcaatat cgtgcaggaa tacaacaccc ttaaagggta tagctggaaa
     1561 tggctgggtg gggtgtcagt ggcttttcta gttatgttct gtgtgctcgg atggggaatg
     1621 aaaacaatgc tcgacaggaa ctacaacgcc acagcggcgg tgtatcagaa agttgtgata
     1681 ctggaagata aactggcgga aaaacctgtc acgggtaaga accggaaaaa ctgacgcgct
     1741 tcgcttgtcc aacgcctcac ggacagcgaa aggatttcgc gttccgttca ccgttttcat
     1801 gagtgattcc tccgaactgc caaaaaatat ccctccctga cttgacagaa aaaataatgg
     1861 cgagtacttt ttgatctgta cataaaacca gtggttttat gtacagtatt aactatgtaa
     1921 ttaattgttt taacgattaa aagagggaat ttttatgagt ggtggagatg gtcgtggccc
     1981 tggtaataca gggcttggac ataacggtgg taaacccagt ggaaacgtga atggctcatc
     2041 tggacatgga ggaccatcat ctggcggtgg gtatcattac aatccgggaa aaggtcctgc
     2101 aagcactgta ggtcctgacg gggaggtgca tatcaacatt accggtggta tggaaaaaga
     2161 tcctccagga aaacatcacg gtaatggtgg ctcaggtaat ggtcacggtg ctggaaatca
     2221 taacagcaat tcaggcggca atggaaaaac aacgtctatt cctggatgga gccctgactc
     2281 tgggaaacct ggcagatatt taccgacaaa taaagataag ttaattaagg gacttattca
     2341 gtcaatagat gaccattctg gaacgtacac tatccattat actggtggtg cagtagctac
     2401 agtaactgta ccaaacggtg atgtgaataa aatgaatgtt cactataata tcggtagtgg
     2461 gccgtcatca ggtaataaac tgactcgtga tactataaaa agctttttag aattcaaggc
     2521 agaaaatgaa gctaatgctg taaatgatgc aataagcaaa gttaatgact tttataaaga
     2581 gttagcatca aaatacggcg ataagttgga gaaagaagcc aaggcactag ccgatgaagc
     2641 taaagggaaa aaaattcgca atgctaatga ggcgattaaa gcctttgata ggtacaagga
     2701 tgtgcttaat aagaaattca gcgtatccga ccgagaagcg attgcaaaag cactggagtc
     2761 tcttaataaa gatcaaatgg ctaaacagtt aaaaattttt ggtaaggcct ttggtgtcgt
     2821 aggtgaggct attcagtggg ggggatttat cagtggctta gttaaaggat tcagaactgg
     2881 tgactggaat gaggctttta taagcgggga aaaaatcgca gtcggcaagg tagcatctgt
     2941 tatggttaca gtggcattta gtgctatggc agtaaatcct attggtattt tagggtttgc
     3001 agtaattatg gcagtgacta gtgccttaat tacagatgaa aggctgaaac aactaaacag
     3061 ctttattaac ggcatttaat aaacaaaagg tcgtaccgtt tgatatggta tgacctttat
     3121 tttgtttttc tatcgctttt tgttggttat aaagtaatag actgtaaaaa tcaatccaaa
     3181 tggaggtagt gataatataa tgcatattat aaaaccccag ttaacgaagt taaaagttgt
     3241 ttttgttagt aagggtgctt gcttatttat tagtaataga caaaaagggt atgtggacga
     3301 tccaaaaaga gatataagta taaatataaa taattctata cttgttgggc ttagattcaa
     3361 acttgcgatg ataaaagata aaatgtaaaa ggataaggca aggataaaat tccttaaata
     3421 gaattccata tctatttttt tcatttataa aatcctcgtt agttaaatgt ttataatatt
     3481 gccttacttt atccataaat ctagaagtag ttatcacaaa agttactttt ttgactcgtt
     3541 cgcccttttg aacggtcgaa actgtttgcg aatcgctttc gtgcatagtt atgcaacgcc
     3601 ctgaaagtga ttctgacgca tttttctggt ttggcctggt gtttccttgt ctttttgcgt
     3661 tttttgcgcc agaaagcgac tgagggcgtt ttaaggggta cgtacaacgg gagttatggt
     3721 aaatggatcg gttttgcggg aaggctcgac aggttttgtt gttgggggta gtgtaagcga
     3781 ctgaaaaaca aacgcccctg aaattcgtgt ctctccgcca agattgtcac gagaatacag
     3841 gggcgaggtc ggtcgataac ctggaaggaa tcgtaacaca tgagcgccgc gcttcaatac
     3901 ttcgaagaaa atttacccca ccgcccctat cacacggatg atctcgcttt cggccttcgc
     3961 atctccggca aagggcgtgc gcttcttgcg cggtacatcc agcagaacca gcctcatgcg
     4021 cagttctggc tggtttttga cgttgaccgc gagggggctg cgattgactg gagcgaccgg
     4081 aacgcccccg cgccgaacat caccgttaaa aatcctgtga acggacacgc tcacctgctc
     4141 tacgcactca acatcgccgt gagaaccgcg cctgatgcct cggttaaggc gctgaaatat
     4201 gccgcagcgg ttgaacgttc gctgtgtgag aaactttgtg ctgatgtgaa ctacagcggc
     4261 ctgatttgca aaaatccgtt ccacctggaa tggcaggtga tggagtggcg cgaggacgcc
     4321 tataccctcg atgaactggc agattatctc gatttgagcg cctcagagcg tcgtagcatc
     4381 gataaacatt acgggatggg gcgaaactgc cacctgttcg aaatgacgcg caaatgggct
     4441 tacagggcga ttcgtcaggg ctggccagcg ttctcacagt ggcttgaagc ggtgatccag
     4501 cgtgtcgaaa tgtacaatgc atcgcttccc gttccgcttt cactggctga atgtcgggct
     4561 attggcaaga gcattgcgaa atacacgcac aggaacttca cgccggaaac tttcgcacag
     4621 tatgtggctg atacgcacac gccagaaatt caggcagcac gcggtcgcaa gggcgggaag
     4681 gccaattcgt cagaaaatca gtcggacaaa gggaaaaaaa gcgccgctgt tcggtggact
     4741 gctaacgacg acaaacgtcg tagggcgctt gatatgtata tcttgggagc gtcaacggaa
     4801 gatattgcag tggcggttgg tgttagcagc cggactattc gacgctggat ggataattct
     4861 ggagaatggc taaccaagaa acaaataatt aaatgttagt taacaaagaa gcaacactac
     4921 cccccgtttt ttgttaatgg gatgttaaat tcgcggacat gaacaagcct atatcagata
     4981 acagcgcctt tcggcgtctt tttgagcagc gggctttccg gcggttttct cctctcagcc
     5041 cagcaatggt gcggcttacc gcatgattga ggtggtcgtg ctcgccgcag tctcatgacc
     5101 gagcgtagcg agcgaatgag cgaggaagca ccagggaacc ggaacacgga tggaggcact
     5161 tacgcgtctg ggtttgggga tgtctccaga tcgtacttaa tttccttaag tgcctgtaat
     5221 atcctgtctt ttttatagca tagctcgcct gtttttgtgt ctaccagtcc tgcttcgatc
     5281 agtagcccag ccgcgagaaa gagtcggtga ttgcgttctc gtcgttgttt ttttccctcg
     5341 attcgttttt ggaccgcctg ttttcgttcg ctctcctgtg cctgtaagtc gtttttttcc
     5401 gctgttgcca gtacgtggag gagtttgcgt tcctgttctg tgagtcctgc ttcgttgtcg
     5461 agc
//
